AlfalfaGEDB | MS.gene014626

M. sativa cultivar XinJiangDaYe / MS.gene014626

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014626.t1	XP_003613438.2	91.2	57	5	0	18	74	28	84	1.40E-19	105.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014626.t1	O04539	80.9	47	9	0	27	73	8	54	4.4e-14	78.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014626.t1	G7K6W4	91.2	57	5	0	18	74	28	84	9.9e-20	105.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene014626.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014626.t1	MTR_5g036670	72.000	75	20	1	1	74	10	84	3.41e-29	108
MS.gene014626.t1	MTR_8g062700	58.571	70	28	1	6	74	14	83	9.16e-20	81.6
MS.gene014626.t1	MTR_8g062700	58.571	70	28	1	6	74	14	83	9.16e-20	81.6
MS.gene014626.t1	MTR_6g008970	70.833	48	14	0	26	73	56	103	1.70e-16	72.4
MS.gene014626.t1	MTR_6g008995	63.830	47	17	0	27	73	31	77	6.60e-13	62.0
MS.gene014626.t1	MTR_1g105955	60.417	48	19	0	26	73	9	56	9.72e-12	57.4
MS.gene014626.t1	MTR_1g104940	61.905	42	16	0	33	74	14	55	1.43e-11	55.8
MS.gene014626.t1	MTR_8g062350	49.020	51	26	0	23	73	4	54	2.57e-11	56.6
MS.gene014626.t1	MTR_1g105960	60.000	40	16	0	35	74	3	42	6.46e-11	53.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene014626.t1	AT1G70060	79.167	48	10	0	26	73	7	54	5.27e-19	79.3
MS.gene014626.t1	AT1G70060	79.167	48	10	0	26	73	7	54	5.27e-19	79.3
MS.gene014626.t1	AT1G24190	72.917	48	13	0	26	73	7	54	3.48e-17	74.3
MS.gene014626.t1	AT1G24190	72.917	48	13	0	26	73	7	54	3.84e-17	73.9
MS.gene014626.t1	AT1G24190	72.917	48	13	0	26	73	7	54	3.92e-17	73.9
MS.gene014626.t1	AT3G01320	68.000	50	16	0	24	73	48	97	6.70e-17	73.6
MS.gene014626.t1	AT3G01320	68.000	50	16	0	24	73	48	97	6.83e-17	73.6
MS.gene014626.t1	AT1G59890	56.522	69	26	1	9	73	17	85	1.48e-15	69.7
MS.gene014626.t1	AT1G59890	56.522	69	26	1	9	73	17	85	1.50e-15	69.7
MS.gene014626.t1	AT1G59890	56.522	69	26	1	9	73	17	85	1.53e-15	69.7
MS.gene014626.t1	AT1G59890	56.522	69	26	1	9	73	17	85	1.67e-15	69.3
MS.gene014626.t1	AT5G15020	65.957	47	16	0	27	73	46	92	1.02e-14	67.4
MS.gene014626.t1	AT5G15020	65.957	47	16	0	27	73	46	92	1.04e-14	67.4
MS.gene014626.t1	AT1G10450	70.455	44	13	0	31	74	31	74	1.78e-14	66.6
MS.gene014626.t1	AT1G10450	70.455	44	13	0	31	74	31	74	1.78e-14	66.6
MS.gene014626.t1	AT1G10450	70.455	44	13	0	31	74	82	125	1.82e-14	66.6
MS.gene014626.t1	AT5G15025	62.222	45	17	0	29	73	43	87	1.51e-13	60.8
MS.gene014626.t1	AT1G24200	52.083	48	23	0	26	73	7	54	1.51e-11	57.0

Find 12 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAATCAAACCTGAGATTGT+TGG	0.415082	3.1:+88171204	None:intergenic
ATCTCGATTCGGCGGTTGCC+CGG	0.418989	3.1:+88171056	None:intergenic
TAAGATCAAGTTTCAAGGTC+CGG	0.429714	3.1:-88171075	MS.gene014626:intron
TTCTCTAAGATCAAGTTTCA+AGG	0.445375	3.1:-88171080	MS.gene014626:intron
ATTAACATGCCAACAATCTC+AGG	0.465297	3.1:-88171213	MS.gene014626:intron
AAGATCAAGTTTCAAGGTCC+GGG	0.505302	3.1:-88171074	MS.gene014626:CDS
AGACAAGAAGGAAAAGTATG+AGG	0.539440	3.1:-88170971	MS.gene014626:CDS
AGCATGTCTCAAGGCGGTCA+AGG	0.604253	3.1:-88171004	MS.gene014626:CDS
GGGCAACCGCCGAATCGAGA+TGG	0.613865	3.1:-88171054	MS.gene014626:CDS
TGATGTTGTAGCATGTCTCA+AGG	0.614364	3.1:-88171013	MS.gene014626:CDS
ATTTGTAGAGTTGATACTGA+TGG	0.633224	3.1:-88170822	MS.gene014626:intron
TGTTGTAGCATGTCTCAAGG+CGG	0.787458	3.1:-88171010	MS.gene014626:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTGTTTTTGTATATATATT+GGG	-	chr3.1:88171140-88171159	MS.gene014626:intron	15.0%
!!	ATATATATACAAAAACAACG+TGG	+	chr3.1:88171139-88171158	None:intergenic	20.0%
!!!	AATTGATTTGTTTATCCTTT+TGG	-	chr3.1:88171189-88171208	MS.gene014626:intron	20.0%
!!!	CGTTGTTTTTGTATATATAT+TGG	-	chr3.1:88171139-88171158	MS.gene014626:intron	20.0%
!	TTTGTATATATATTGGGAGA+GGG	-	chr3.1:88171146-88171165	MS.gene014626:intron	25.0%
!!	TTTTGTATATATATTGGGAG+AGG	-	chr3.1:88171145-88171164	MS.gene014626:intron	25.0%
!!!	TGAAAGATTTTAAAGCACAA+AGG	-	chr3.1:88171104-88171123	MS.gene014626:intron	25.0%
	TCATTTCTTTATTGCTCCTT+CGG	+	chr3.1:88170881-88170900	None:intergenic	30.0%
	TTAATCAAACCTGAGATTGT+TGG	+	chr3.1:88170836-88170855	None:intergenic	30.0%
	TTGAGTGTGATCTTATCATA+TGG	+	chr3.1:88170920-88170939	None:intergenic	30.0%
!!	ATTTGTAGAGTTGATACTGA+TGG	-	chr3.1:88171215-88171234	MS.gene014626:CDS	30.0%
!!	TTCTCTAAGATCAAGTTTCA+AGG	-	chr3.1:88170957-88170976	MS.gene014626:CDS	30.0%
!!!	GGATATTTTTCAAGACAAGA+AGG	-	chr3.1:88171054-88171073	MS.gene014626:CDS	30.0%
	AACTCTACAAATGCACCAAA+AGG	+	chr3.1:88171207-88171226	None:intergenic	35.0%
	ATTAACATGCCAACAATCTC+AGG	-	chr3.1:88170824-88170843	MS.gene014626:intron	35.0%
	TAAGATCAAGTTTCAAGGTC+CGG	-	chr3.1:88170962-88170981	MS.gene014626:CDS	35.0%
!	AAAGTATGAGGACTTTTTGG+AGG	-	chr3.1:88171078-88171097	MS.gene014626:intron	35.0%
!	AGACAAGAAGGAAAAGTATG+AGG	-	chr3.1:88171066-88171085	MS.gene014626:CDS	35.0%
!	AGTTTTTGACCATCTCGATT+CGG	+	chr3.1:88170995-88171014	None:intergenic	35.0%
!!	GGAAAAGTATGAGGACTTTT+TGG	-	chr3.1:88171075-88171094	MS.gene014626:intron	35.0%
	AAGATCAAGTTTCAAGGTCC+GGG	-	chr3.1:88170963-88170982	MS.gene014626:CDS	40.0%
	GATCACACTCAACATGATTG+TGG	-	chr3.1:88170928-88170947	MS.gene014626:intron	40.0%
	TGATGTTGTAGCATGTCTCA+AGG	-	chr3.1:88171024-88171043	MS.gene014626:CDS	40.0%
	TGTTGTAGCATGTCTCAAGG+CGG	-	chr3.1:88171027-88171046	MS.gene014626:CDS	45.0%
	TTTATTGCTCCTTCGGCTCT+AGG	+	chr3.1:88170874-88170893	None:intergenic	45.0%
!!!	TTTTGACCATCTCGATTCGG+CGG	+	chr3.1:88170992-88171011	None:intergenic	45.0%
	TACTTAGTGCCTAGAGCCGA+AGG	-	chr3.1:88170862-88170881	MS.gene014626:intron	50.0%
	AGCATGTCTCAAGGCGGTCA+AGG	-	chr3.1:88171033-88171052	MS.gene014626:CDS	55.0%
!!	ATCTCGATTCGGCGGTTGCC+CGG	+	chr3.1:88170984-88171003	None:intergenic	60.0%
	GGGCAACCGCCGAATCGAGA+TGG	-	chr3.1:88170983-88171002	MS.gene014626:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	88170809	88171250	88170809	ID=MS.gene014626
chr3.1	mRNA	88170809	88171250	88170809	ID=MS.gene014626.t1;Parent=MS.gene014626
chr3.1	exon	88171214	88171250	88171214	ID=MS.gene014626.t1.exon1;Parent=MS.gene014626.t1
chr3.1	CDS	88171214	88171250	88171214	ID=cds.MS.gene014626.t1;Parent=MS.gene014626.t1
chr3.1	exon	88170934	88171093	88170934	ID=MS.gene014626.t1.exon2;Parent=MS.gene014626.t1
chr3.1	CDS	88170934	88171093	88170934	ID=cds.MS.gene014626.t1;Parent=MS.gene014626.t1
chr3.1	exon	88170809	88170836	88170809	ID=MS.gene014626.t1.exon3;Parent=MS.gene014626.t1
chr3.1	CDS	88170809	88170836	88170809	ID=cds.MS.gene014626.t1;Parent=MS.gene014626.t1

Gene Sequence

>MS.gene014626

ATGAATTGTGAAGACATTAACATGCCAACAATCTCAGGTTTGATTAAGATAAATACTTAGTGCCTAGAGCCGAAGGAGCAATAAAGAAATGAAAACATAATTCAGAAACCATATGATAAGATCACACTCAACATGATTGTGGTGTATATTCTCTAAGATCAAGTTTCAAGGTCCGGGCAACCGCCGAATCGAGATGGTCAAAAACTGACTACTAATGATGTTGTAGCATGTCTCAAGGCGGTCAAGGATATTTTTCAAGACAAGAAGGAAAAGTATGAGGACTTTTTGGAGGTTATGAAAGATTTTAAAGCACAAAGGTATTTTGTACCACGTTGTTTTTGTATATATATTGGGAGAGGGATTATGAATTTGTTAGTTGTAATTGATTTGTTTATCCTTTTGGTGCATTTGTAGAGTTGATACTGATGGTGTCATTGAATGA

Protein sequence

>MS.gene014626.t1

MNCEDINMPTISDQVSRSGQPPNRDGQKLTTNDVVACLKAVKDIFQDKKEKYEDFLEVMKDFKAQRVDTDGVIE