Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014681.t1 | KEH35976.1 | 80.1 | 166 | 33 | 0 | 25 | 190 | 10 | 175 | 1.60E-64 | 255.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014681.t1 | Q9FKK2 | 27.5 | 160 | 111 | 1 | 11 | 165 | 1 | 160 | 1.9e-13 | 77.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014681.t1 | A0A072V2S9 | 80.1 | 166 | 33 | 0 | 25 | 190 | 10 | 175 | 1.2e-64 | 255.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014681.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014681.t1 | MTR_3g109930 | 80.120 | 166 | 33 | 0 | 25 | 190 | 10 | 175 | 3.90e-92 | 266 |
| MS.gene014681.t1 | MTR_8g022970 | 70.270 | 185 | 53 | 2 | 1 | 183 | 1 | 185 | 8.42e-85 | 248 |
| MS.gene014681.t1 | MTR_3g437790 | 44.444 | 171 | 88 | 5 | 13 | 179 | 11 | 178 | 2.46e-38 | 130 |
| MS.gene014681.t1 | MTR_2g045020 | 46.296 | 162 | 74 | 5 | 14 | 171 | 19 | 171 | 3.55e-30 | 109 |
| MS.gene014681.t1 | MTR_2g105290 | 38.125 | 160 | 91 | 5 | 13 | 169 | 8 | 162 | 8.20e-24 | 92.8 |
| MS.gene014681.t1 | MTR_7g028448 | 36.567 | 134 | 75 | 2 | 35 | 168 | 30 | 153 | 2.96e-21 | 85.9 |
| MS.gene014681.t1 | MTR_4g036915 | 36.567 | 134 | 75 | 2 | 35 | 168 | 30 | 153 | 2.96e-21 | 85.9 |
| MS.gene014681.t1 | MTR_1g012570 | 34.545 | 165 | 95 | 5 | 13 | 170 | 5 | 163 | 5.10e-19 | 81.6 |
| MS.gene014681.t1 | MTR_5g053390 | 35.256 | 156 | 96 | 3 | 18 | 170 | 10 | 163 | 2.33e-18 | 80.1 |
| MS.gene014681.t1 | MTR_8g086290 | 31.214 | 173 | 97 | 4 | 7 | 169 | 2 | 162 | 1.10e-17 | 77.0 |
| MS.gene014681.t1 | MTR_1g106070 | 32.727 | 165 | 98 | 4 | 13 | 170 | 5 | 163 | 2.28e-17 | 76.3 |
| MS.gene014681.t1 | MTR_2g030740 | 33.533 | 167 | 94 | 6 | 13 | 170 | 5 | 163 | 8.10e-17 | 75.9 |
| MS.gene014681.t1 | MTR_3g052920 | 33.951 | 162 | 91 | 4 | 13 | 165 | 5 | 159 | 3.52e-16 | 73.9 |
| MS.gene014681.t1 | MTR_5g055100 | 32.275 | 189 | 92 | 7 | 18 | 189 | 10 | 179 | 6.68e-16 | 73.6 |
| MS.gene014681.t1 | MTR_1g105910 | 30.128 | 156 | 105 | 2 | 13 | 165 | 5 | 159 | 1.19e-15 | 72.8 |
| MS.gene014681.t1 | MTR_8g043650 | 29.936 | 157 | 104 | 3 | 13 | 165 | 5 | 159 | 1.21e-15 | 72.8 |
| MS.gene014681.t1 | MTR_1g105920 | 30.189 | 159 | 101 | 3 | 13 | 165 | 5 | 159 | 3.70e-15 | 71.2 |
| MS.gene014681.t1 | MTR_1g108580 | 28.962 | 183 | 126 | 2 | 10 | 189 | 2 | 183 | 5.97e-15 | 70.9 |
| MS.gene014681.t1 | MTR_1g108510 | 29.444 | 180 | 113 | 5 | 18 | 189 | 10 | 183 | 7.03e-15 | 70.5 |
| MS.gene014681.t1 | MTR_3g030770 | 28.492 | 179 | 116 | 3 | 18 | 189 | 10 | 183 | 9.56e-15 | 70.1 |
| MS.gene014681.t1 | MTR_2g093190 | 28.977 | 176 | 119 | 3 | 18 | 189 | 10 | 183 | 2.10e-14 | 69.3 |
| MS.gene014681.t1 | MTR_4g127140 | 31.944 | 144 | 88 | 2 | 35 | 169 | 30 | 172 | 2.89e-14 | 68.2 |
| MS.gene014681.t1 | MTR_8g079502 | 33.628 | 113 | 68 | 2 | 18 | 126 | 3 | 112 | 3.05e-14 | 67.8 |
| MS.gene014681.t1 | MTR_2g085280 | 31.410 | 156 | 93 | 4 | 18 | 165 | 10 | 159 | 4.08e-14 | 68.6 |
| MS.gene014681.t1 | MTR_5g045560 | 31.677 | 161 | 105 | 3 | 18 | 175 | 10 | 168 | 4.12e-14 | 68.6 |
| MS.gene014681.t1 | MTR_2g085250 | 31.410 | 156 | 93 | 4 | 18 | 165 | 10 | 159 | 4.51e-14 | 68.6 |
| MS.gene014681.t1 | MTR_3g080940 | 27.528 | 178 | 117 | 5 | 18 | 189 | 10 | 181 | 2.33e-13 | 65.9 |
| MS.gene014681.t1 | MTR_1g047550 | 34.906 | 106 | 68 | 1 | 18 | 122 | 3 | 108 | 2.67e-13 | 65.5 |
| MS.gene014681.t1 | MTR_1g105905 | 29.927 | 137 | 90 | 3 | 32 | 164 | 7 | 141 | 1.10e-12 | 64.3 |
| MS.gene014681.t1 | MTR_4g131030 | 36.842 | 95 | 59 | 1 | 18 | 111 | 3 | 97 | 2.40e-11 | 60.8 |
| MS.gene014681.t1 | MTR_1g075600 | 29.747 | 158 | 93 | 4 | 18 | 165 | 10 | 159 | 4.15e-11 | 60.5 |
| MS.gene014681.t1 | MTR_3g052870 | 37.333 | 75 | 47 | 0 | 13 | 87 | 5 | 79 | 8.85e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014681.t1 | AT5G60440 | 27.500 | 160 | 111 | 1 | 11 | 165 | 1 | 160 | 3.06e-17 | 78.2 |
| MS.gene014681.t1 | AT2G24840 | 27.215 | 158 | 112 | 2 | 18 | 172 | 64 | 221 | 3.36e-15 | 72.0 |
| MS.gene014681.t1 | AT1G65360 | 28.931 | 159 | 106 | 2 | 11 | 165 | 1 | 156 | 3.78e-15 | 71.2 |
| MS.gene014681.t1 | AT1G01530 | 29.348 | 184 | 116 | 4 | 11 | 185 | 1 | 179 | 5.02e-15 | 71.2 |
| MS.gene014681.t1 | AT2G34440 | 28.655 | 171 | 108 | 4 | 18 | 183 | 3 | 164 | 7.17e-14 | 66.6 |
| MS.gene014681.t1 | AT3G66656 | 40.299 | 67 | 40 | 0 | 18 | 84 | 3 | 69 | 4.84e-12 | 62.0 |
| MS.gene014681.t1 | AT4G36590 | 27.168 | 173 | 112 | 4 | 11 | 172 | 1 | 170 | 5.43e-11 | 60.1 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAGCTATCGACGCTGAAAA+AGG | 0.238437 | 3.1:-87313427 | MS.gene014681:CDS |
| AAGTGTTTAATGACGATGTT+AGG | 0.253299 | 3.1:+87313466 | None:intergenic |
| ATAGTTCATGTCTTCCTTCT+TGG | 0.278512 | 3.1:-87313294 | MS.gene014681:CDS |
| ATTGAATAGTTATATAATAT+TGG | 0.279123 | 3.1:+87313135 | None:intergenic |
| CTCTGCTTTCTTGATTTCAA+TGG | 0.286601 | 3.1:+87313636 | None:intergenic |
| TGTCTCAAGTTGAAGCCTTA+AGG | 0.358087 | 3.1:+87313378 | None:intergenic |
| TTCTATTGATGATATGACTT+TGG | 0.396231 | 3.1:-87313263 | MS.gene014681:CDS |
| GCTTAATTTAGTTGCTTCCT+TGG | 0.408024 | 3.1:-87313200 | MS.gene014681:CDS |
| TAATGACGATGTTAGGGTTA+GGG | 0.414307 | 3.1:+87313473 | None:intergenic |
| AGGACACAATTGAAACCTTA+AGG | 0.422757 | 3.1:-87313393 | MS.gene014681:CDS |
| TTAATGACGATGTTAGGGTT+AGG | 0.425753 | 3.1:+87313472 | None:intergenic |
| GTTCATGTCTTCCTTCTTGG+TGG | 0.441280 | 3.1:-87313291 | MS.gene014681:CDS |
| AATTTAGTTGCTTCCTTGGA+AGG | 0.461652 | 3.1:-87313196 | MS.gene014681:CDS |
| TTCATGTCTTCCTTCTTGGT+GGG | 0.465660 | 3.1:-87313290 | MS.gene014681:CDS |
| AGTGTTTAATGACGATGTTA+GGG | 0.483491 | 3.1:+87313467 | None:intergenic |
| TTTCCATCTGAAGAAGCCAT+TGG | 0.494665 | 3.1:+87313697 | None:intergenic |
| TATAATATTGGAAGTTGAAG+AGG | 0.495356 | 3.1:+87313147 | None:intergenic |
| GTGAATCTCTTTCCCTTCCA+AGG | 0.502904 | 3.1:+87313183 | None:intergenic |
| GATCCAATGGCTTCTTCAGA+TGG | 0.507351 | 3.1:-87313700 | None:intergenic |
| ATTTAGTTGCTTCCTTGGAA+GGG | 0.542159 | 3.1:-87313195 | MS.gene014681:CDS |
| ACTTTCTCAAAGCTCAAACT+AGG | 0.549629 | 3.1:-87313592 | MS.gene014681:CDS |
| TCAAGAACGACTGAAAGAAG+AGG | 0.586071 | 3.1:-87313353 | MS.gene014681:CDS |
| ATAGAATGATCCCACCAAGA+AGG | 0.601023 | 3.1:+87313280 | None:intergenic |
| GAAGAGGAGAACAATTTACA+AGG | 0.654527 | 3.1:-87313337 | MS.gene014681:CDS |
| GCATAGAATAGATAATTCTG+TGG | 0.670141 | 3.1:+87313555 | None:intergenic |
| GGAAGGGAAAGAGATTCACA+TGG | 0.692812 | 3.1:-87313179 | MS.gene014681:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TATAATATTGGAAGTTGAAG+AGG | + | chr3.1:87313694-87313713 | None:intergenic | 25.0% |
| !! | TTCTATTGATGATATGACTT+TGG | - | chr3.1:87313575-87313594 | MS.gene014681:CDS | 25.0% |
| !!! | ATCGTCATTAAACACTTTTT+CGG | - | chr3.1:87313378-87313397 | MS.gene014681:CDS | 25.0% |
| AAGTGTTTAATGACGATGTT+AGG | + | chr3.1:87313375-87313394 | None:intergenic | 30.0% | |
| AGTGTTTAATGACGATGTTA+GGG | + | chr3.1:87313374-87313393 | None:intergenic | 30.0% | |
| GCATAGAATAGATAATTCTG+TGG | + | chr3.1:87313286-87313305 | None:intergenic | 30.0% | |
| !!! | CATTAAACACTTTTTCGGTA+AGG | - | chr3.1:87313383-87313402 | MS.gene014681:CDS | 30.0% |
| AATTTAGTTGCTTCCTTGGA+AGG | - | chr3.1:87313642-87313661 | MS.gene014681:CDS | 35.0% | |
| ACTTTCTCAAAGCTCAAACT+AGG | - | chr3.1:87313246-87313265 | MS.gene014681:CDS | 35.0% | |
| AGGACACAATTGAAACCTTA+AGG | - | chr3.1:87313445-87313464 | MS.gene014681:CDS | 35.0% | |
| ATAGTTCATGTCTTCCTTCT+TGG | - | chr3.1:87313544-87313563 | MS.gene014681:CDS | 35.0% | |
| ATTTAGTTGCTTCCTTGGAA+GGG | - | chr3.1:87313643-87313662 | MS.gene014681:CDS | 35.0% | |
| CTCTGCTTTCTTGATTTCAA+TGG | + | chr3.1:87313205-87313224 | None:intergenic | 35.0% | |
| GAAGAGGAGAACAATTTACA+AGG | - | chr3.1:87313501-87313520 | MS.gene014681:CDS | 35.0% | |
| GACAAAAAACTCTATGCATG+TGG | - | chr3.1:87313339-87313358 | MS.gene014681:CDS | 35.0% | |
| GCTTAATTTAGTTGCTTCCT+TGG | - | chr3.1:87313638-87313657 | MS.gene014681:CDS | 35.0% | |
| TAATGACGATGTTAGGGTTA+GGG | + | chr3.1:87313368-87313387 | None:intergenic | 35.0% | |
| TTAATGACGATGTTAGGGTT+AGG | + | chr3.1:87313369-87313388 | None:intergenic | 35.0% | |
| ATAGAATGATCCCACCAAGA+AGG | + | chr3.1:87313561-87313580 | None:intergenic | 40.0% | |
| TCAAGAACGACTGAAAGAAG+AGG | - | chr3.1:87313485-87313504 | MS.gene014681:CDS | 40.0% | |
| TGAAAAAGGGAAACAGGATG+AGG | - | chr3.1:87313425-87313444 | MS.gene014681:CDS | 40.0% | |
| TTCATGTCTTCCTTCTTGGT+GGG | - | chr3.1:87313548-87313567 | MS.gene014681:CDS | 40.0% | |
| !! | TGTCTCAAGTTGAAGCCTTA+AGG | + | chr3.1:87313463-87313482 | None:intergenic | 40.0% |
| CAGCTATCGACGCTGAAAAA+GGG | - | chr3.1:87313412-87313431 | MS.gene014681:CDS | 45.0% | |
| GGAAGGGAAAGAGATTCACA+TGG | - | chr3.1:87313659-87313678 | MS.gene014681:CDS | 45.0% | |
| GTGAATCTCTTTCCCTTCCA+AGG | + | chr3.1:87313658-87313677 | None:intergenic | 45.0% | |
| GTTCATGTCTTCCTTCTTGG+TGG | - | chr3.1:87313547-87313566 | MS.gene014681:CDS | 45.0% | |
| TCAGCTATCGACGCTGAAAA+AGG | - | chr3.1:87313411-87313430 | MS.gene014681:CDS | 45.0% | |
| CGACGCTGAAAAAGGGAAAC+AGG | - | chr3.1:87313419-87313438 | MS.gene014681:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 87313144 | 87313716 | 87313144 | ID=MS.gene014681 |
| chr3.1 | mRNA | 87313144 | 87313716 | 87313144 | ID=MS.gene014681.t1;Parent=MS.gene014681 |
| chr3.1 | exon | 87313144 | 87313716 | 87313144 | ID=MS.gene014681.t1.exon1;Parent=MS.gene014681.t1 |
| chr3.1 | CDS | 87313144 | 87313716 | 87313144 | ID=cds.MS.gene014681.t1;Parent=MS.gene014681.t1 |
| Gene Sequence |
| Protein sequence |
