AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01482

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01482.t1 MTR_2g014680 94.498 309 15 2 1 308 1 308 0.0 608
MS.gene01482.t1 MTR_4g088245 61.656 326 88 9 1 302 1 313 2.74e-135 386
MS.gene01482.t1 MTR_8g069160 69.027 226 49 2 1 208 1 223 8.69e-114 329
MS.gene01482.t1 MTR_4g078875 58.803 284 102 7 1 279 1 274 1.62e-108 317
MS.gene01482.t1 MTR_2g090735 57.877 292 110 6 1 290 1 281 6.14e-106 310
MS.gene01482.t1 MTR_8g102240 61.572 229 74 5 8 226 3 227 1.32e-88 273
MS.gene01482.t1 MTR_8g102240 61.572 229 74 5 8 226 3 227 1.35e-88 273
MS.gene01482.t1 MTR_5g053430 58.247 194 78 2 27 220 34 224 5.94e-73 231
MS.gene01482.t1 MTR_5g053430 58.247 194 78 2 27 220 34 224 8.76e-73 231
MS.gene01482.t1 MTR_1g093680 50.633 237 111 4 2 235 7 240 3.97e-72 230
MS.gene01482.t1 MTR_7g033320 56.216 185 74 3 31 212 35 215 1.04e-66 217
MS.gene01482.t1 MTR_1g093670 47.291 203 86 3 32 233 7 189 3.04e-50 170
MS.gene01482.t1 MTR_4g078895 60.000 65 24 1 30 94 16 78 1.26e-19 82.0
MS.gene01482.t1 MTR_2g014700 68.889 45 14 0 118 162 44 88 4.41e-16 72.8
MS.gene01482.t1 MTR_6g084430 32.317 164 92 6 53 213 5 152 4.80e-15 74.7
MS.gene01482.t1 MTR_7g011130 30.457 197 120 4 36 231 2 182 1.20e-14 73.9
MS.gene01482.t1 MTR_3g070030 30.337 178 100 5 41 210 7 168 2.05e-14 73.2
MS.gene01482.t1 MTR_7g085260 31.111 180 93 9 46 212 10 171 3.81e-13 69.7
MS.gene01482.t1 MTR_3g109340 31.461 178 100 7 38 210 8 168 4.42e-13 69.3
MS.gene01482.t1 MTR_3g109340 31.285 179 100 7 38 210 8 169 5.46e-13 68.9
MS.gene01482.t1 MTR_6g032770 34.104 173 77 11 53 210 18 168 6.55e-13 68.9
MS.gene01482.t1 MTR_3g022390 83.333 36 6 0 193 228 38 73 6.83e-13 63.5
MS.gene01482.t1 MTR_3g435150 33.803 142 77 5 53 193 16 141 7.63e-13 68.2
MS.gene01482.t1 MTR_7g005280 33.333 147 70 8 53 193 15 139 2.83e-12 65.9
MS.gene01482.t1 MTR_1g069805 27.700 213 121 9 52 253 9 199 2.14e-11 63.5
MS.gene01482.t1 MTR_8g099750 32.000 150 79 8 48 193 9 139 2.36e-11 62.8
MS.gene01482.t1 MTR_8g023880 33.974 156 74 8 52 192 6 147 2.36e-11 63.5
MS.gene01482.t1 MTR_2g078700 32.738 168 86 9 53 210 23 173 2.40e-11 64.3
MS.gene01482.t1 MTR_3g093040 29.208 202 107 10 50 244 105 277 3.27e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01482.t1 AT4G28500 64.378 233 68 3 1 222 1 229 5.20e-107 314
MS.gene01482.t1 AT1G28470 58.246 285 79 7 1 249 1 281 9.85e-107 313
MS.gene01482.t1 AT4G29230 62.332 223 69 3 6 217 2 220 1.00e-91 280
MS.gene01482.t1 AT4G29230 62.332 223 69 3 6 217 2 220 3.81e-91 280
MS.gene01482.t1 AT4G29230 61.435 223 70 3 6 217 2 219 5.25e-90 277
MS.gene01482.t1 AT5G56620 59.091 220 75 5 9 217 3 218 4.29e-82 253
MS.gene01482.t1 AT5G56620 59.091 220 75 5 9 217 3 218 4.43e-82 254
MS.gene01482.t1 AT5G56620 59.091 220 75 5 9 217 3 218 5.48e-82 254
MS.gene01482.t1 AT1G25580 57.068 191 73 4 27 217 34 215 1.46e-69 223
MS.gene01482.t1 AT5G14490 50.500 200 94 4 30 228 34 229 1.56e-56 184
MS.gene01482.t1 AT5G14490 50.500 200 94 4 30 228 34 229 4.19e-56 185
MS.gene01482.t1 AT5G14490 50.500 200 94 4 30 228 37 232 4.34e-56 185
MS.gene01482.t1 AT5G14490 50.500 200 94 4 30 228 37 232 6.27e-56 185
MS.gene01482.t1 AT3G01600 49.462 186 90 3 30 214 34 216 1.20e-50 171
MS.gene01482.t1 AT3G04060 32.609 184 98 7 41 215 8 174 4.73e-19 86.7
MS.gene01482.t1 AT5G18270 33.514 185 94 10 41 215 10 175 2.82e-16 78.6
MS.gene01482.t1 AT5G18270 33.514 185 94 10 41 215 10 175 4.34e-16 78.2
MS.gene01482.t1 AT3G18400 33.803 142 77 5 53 193 5 130 7.98e-15 73.9
MS.gene01482.t1 AT1G33060 30.645 186 94 9 40 212 11 174 9.17e-14 72.0
MS.gene01482.t1 AT1G33060 30.645 186 94 9 40 212 11 174 9.28e-14 72.0
MS.gene01482.t1 AT4G35580 31.551 187 93 10 46 219 2 166 4.41e-13 70.1
MS.gene01482.t1 AT4G35580 31.551 187 93 10 46 219 2 166 5.70e-13 69.7
MS.gene01482.t1 AT4G35580 31.551 187 93 10 46 219 2 166 5.79e-13 69.7
MS.gene01482.t1 AT2G24430 29.586 169 97 6 52 214 15 167 2.60e-12 66.6
MS.gene01482.t1 AT2G24430 29.586 169 97 6 52 214 15 167 2.60e-12 66.6
MS.gene01482.t1 AT1G01720 30.464 151 83 6 47 194 1 132 2.12e-11 63.9
MS.gene01482.t1 AT1G76420 31.492 181 98 10 52 224 21 183 2.21e-11 64.3
MS.gene01482.t1 AT3G29035 32.867 143 77 5 53 193 24 149 2.83e-11 63.9
MS.gene01482.t1 AT2G33480 29.412 187 98 10 41 221 7 165 5.13e-11 62.4
MS.gene01482.t1 AT2G33480 29.101 189 98 10 41 221 7 167 6.37e-11 62.4

Find 58 sgRNAs with CRISPR-Local

Find 156 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAATCTCTTACATTGTTAA+TGG 0.179782 2.2:+68335753 None:intergenic
GAAAACCAGAGAAGACTAAT+TGG 0.273159 2.2:-68335879 MS.gene01482:CDS
GAAACAAGATGGCACAAAAC+TGG 0.293534 2.2:-68335985 MS.gene01482:CDS
TCTAGTACTTCTACATCATT+TGG 0.314489 2.2:+68337953 None:intergenic
CCAACGCTTCAAGGAGATAA+TGG 0.355602 2.2:-68337866 MS.gene01482:CDS
GAAGGGATTTAAGAAGATAT+TGG 0.357820 2.2:-68335929 MS.gene01482:CDS
TGTAGAAGTACTAGAGCATT+TGG 0.390413 2.2:-68337945 MS.gene01482:CDS
ATCACCCAATTAGTCTTCTC+TGG 0.399788 2.2:+68335874 None:intergenic
TCACTTTGAACAGCCATGTT+TGG 0.400365 2.2:+68335574 None:intergenic
AACTATTATTCGCTTATCAA+TGG 0.414728 2.2:-68335548 MS.gene01482:CDS
GGGTGGAGTTAATGAATTGC+CGG 0.417191 2.2:-68338002 MS.gene01482:CDS
CACATTAAGAGCTACATTGT+TGG 0.427511 2.2:+68335678 None:intergenic
TGTTCCTGTTGTGTATGCTT+TGG 0.432715 2.2:+68336047 None:intergenic
ATATGCATGTTGGTCATAGC+AGG 0.451891 2.2:-68335621 MS.gene01482:CDS
CTAGTACTTCTACATCATTT+GGG 0.458512 2.2:+68337954 None:intergenic
GAGTTCATACCAACGCTTCA+AGG 0.459148 2.2:-68337875 MS.gene01482:CDS
CCATTATCTCCTTGAAGCGT+TGG 0.461132 2.2:+68337866 None:intergenic
GGGTCAAATTTCACTCCAGC+TGG 0.465906 2.2:+68337974 None:intergenic
CTTCTTCATTCATACCAAGA+TGG 0.471328 2.2:+68335842 None:intergenic
CCTGTTGTGTATGCTTTGGA+AGG 0.474842 2.2:+68336051 None:intergenic
TACACCAACTATTGCAAAGA+AGG 0.482059 2.2:-68335901 MS.gene01482:CDS
TGCTATGACCAACATGCATA+TGG 0.484398 2.2:+68335623 None:intergenic
CCTTCCAAAGCATACACAAC+AGG 0.484981 2.2:-68336051 MS.gene01482:CDS
TGCAAACAAAGTAAGAGTAC+TGG 0.494119 2.2:-68335521 MS.gene01482:CDS
GAATTGCCGGGGTTACCAGC+TGG 0.498475 2.2:-68337989 MS.gene01482:CDS
GCCGCATGAGGGGCATTCAA+TGG 0.498697 2.2:+68338276 None:intergenic
GGTGGAGTTAATGAATTGCC+GGG 0.501114 2.2:-68338001 MS.gene01482:CDS
TTCAATATTATGGCCGCATG+AGG 0.506854 2.2:+68338264 None:intergenic
AAAACCAGAGAAGACTAATT+GGG 0.508959 2.2:-68335878 MS.gene01482:CDS
AAAATAATCAACAAAGGAAG+TGG 0.515719 2.2:-68335724 MS.gene01482:CDS
TCAATATTATGGCCGCATGA+GGG 0.518397 2.2:+68338265 None:intergenic
GTTGCAGGAGTTAGCAAAGA+TGG 0.520564 2.2:-68336099 MS.gene01482:intron
ATGGAATTACCATGTTGTCT+AGG 0.524147 2.2:+68335772 None:intergenic
AACATGGCTGTTCAAAGTGA+TGG 0.524451 2.2:-68335571 MS.gene01482:CDS
ACACACCCAGCACAATTACC+AGG 0.525873 2.2:-68337833 MS.gene01482:intron
ATTAAGAGCTACATTGTTGG+AGG 0.531511 2.2:+68335681 None:intergenic
GTGATGCATCAATACCATCT+TGG 0.536511 2.2:-68335856 MS.gene01482:CDS
AAGAAGGAATTGAAACAAGA+TGG 0.540463 2.2:-68335996 MS.gene01482:CDS
ATCAACAAAGGAAGTGGCCA+TGG 0.542226 2.2:-68335718 MS.gene01482:CDS
ATGTTTGGTATAATTAGTTG+TGG 0.542896 2.2:+68335589 None:intergenic
ATGAATGAAGAAGAAAGAGA+TGG 0.549873 2.2:-68335832 MS.gene01482:CDS
AAACAAGATGGCACAAAACT+GGG 0.551473 2.2:-68335984 MS.gene01482:CDS
TTCACTCCAGCTGGTAACCC+CGG 0.559582 2.2:+68337983 None:intergenic
AAGGTTCACACTGATGAAGA+AGG 0.564034 2.2:-68336012 MS.gene01482:CDS
CAATATTATGGCCGCATGAG+GGG 0.567135 2.2:+68338266 None:intergenic
AGGAACAAGGAAAAGAAGAA+AGG 0.568088 2.2:-68336031 MS.gene01482:CDS
CAACAATGTAGCTCTTAATG+TGG 0.583204 2.2:-68335677 MS.gene01482:CDS
GGTATGAACTCATCAATAAG+TGG 0.587548 2.2:+68337887 None:intergenic
CAGACACAGCCTAGACAACA+TGG 0.594185 2.2:-68335781 MS.gene01482:CDS
AATTATGACCATATGCATGT+TGG 0.599378 2.2:-68335631 MS.gene01482:CDS
TCCATTGAATGCCCCTCATG+CGG 0.616642 2.2:-68338277 MS.gene01482:CDS
AGAAGTACTAGAGCATTTGG+AGG 0.620573 2.2:-68337942 MS.gene01482:CDS
ACAACTAATTATACCAAACA+TGG 0.621621 2.2:-68335587 MS.gene01482:CDS
AAGCATACACAACAGGAACA+AGG 0.635238 2.2:-68336044 MS.gene01482:CDS
AGGCTGCACTGTTATTACCA+TGG 0.642479 2.2:+68335701 None:intergenic
AGAGATTAAGAACTATGGCA+TGG 0.652933 2.2:-68338375 None:intergenic
TATGCATGTTGGTCATAGCA+GGG 0.654809 2.2:-68335620 MS.gene01482:CDS
GTGGAGTTAATGAATTGCCG+GGG 0.680068 2.2:-68338000 MS.gene01482:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTATAATTTTTTTTTTTT+TGG - chr2.2:68336215-68336234 MS.gene01482:intron 0.0%
!! TTAAATTAAATATAAAAACC+TGG + chr2.2:68336059-68336078 None:intergenic 10.0%
!!! TATAGATTAGTTAAAAAATA+TGG - chr2.2:68336424-68336443 MS.gene01482:intron 10.0%
!!! TGAAATAGATTTTAATTATA+AGG - chr2.2:68336545-68336564 MS.gene01482:intron 10.0%
!! AAAGAAAATTATTTGTCAAT+TGG - chr2.2:68336649-68336668 MS.gene01482:intron 15.0%
!! AAGAAAATTATTTGTCAATT+GGG - chr2.2:68336650-68336669 MS.gene01482:intron 15.0%
!! ATTAAAATGATTTAGAGATT+TGG - chr2.2:68336098-68336117 MS.gene01482:intron 15.0%
!! TAAACTAAAGGAATTAATAT+CGG + chr2.2:68335812-68335831 None:intergenic 15.0%
!! TAATATTGAAGTAAAAAATC+AGG - chr2.2:68335620-68335639 MS.gene01482:CDS 15.0%
!! TATGAAAAAATAATCAACAA+AGG - chr2.2:68338141-68338160 MS.gene01482:intron 15.0%
!! TTAATATTACATTGAGAAAA+AGG - chr2.2:68337683-68337702 MS.gene01482:intron 15.0%
!!! AATAGATTTTAATTATAAGG+CGG - chr2.2:68336548-68336567 MS.gene01482:intron 15.0%
!!! AATTAATTAATTTGATGCAT+GGG + chr2.2:68336187-68336206 None:intergenic 15.0%
!!! GATTTTTTACTTCAATATTA+TGG + chr2.2:68335620-68335639 None:intergenic 15.0%
!!! TAATTAATTAATTTGATGCA+TGG + chr2.2:68336188-68336207 None:intergenic 15.0%
!! AAGTGAATAAGAATTTGTAA+AGG + chr2.2:68337482-68337501 None:intergenic 20.0%
!! ACACAAAAAAAAAAGGTAAT+TGG + chr2.2:68337126-68337145 None:intergenic 20.0%
!! AGAAAATTATTTGTCAATTG+GGG - chr2.2:68336651-68336670 MS.gene01482:intron 20.0%
!! CACACAAATTAATAAACTAA+AGG + chr2.2:68335824-68335843 None:intergenic 20.0%
!! GTGATACATTATTAATCAAA+AGG + chr2.2:68337198-68337217 None:intergenic 20.0%
!!! AAAAATTGTTCCTTAAGTTT+TGG - chr2.2:68337096-68337115 MS.gene01482:intron 20.0%
!!! ATTAATTAATTTGATGCATG+GGG + chr2.2:68336186-68336205 None:intergenic 20.0%
!!! ATTACCTTTTTTTTTTGTGT+TGG - chr2.2:68337126-68337145 MS.gene01482:intron 20.0%
!!! ATTTTCATAAGTTAGTAGAA+AGG + chr2.2:68336693-68336712 None:intergenic 20.0%
!!! GAAATATATTGACTTGAAAA+CGG - chr2.2:68337614-68337633 MS.gene01482:intron 20.0%
!!! TAATTTGTTACTGTAACATT+AGG - chr2.2:68337045-68337064 MS.gene01482:intron 20.0%
!!! TGATTATTTTTTGTGAATTG+TGG - chr2.2:68336757-68336776 MS.gene01482:intron 20.0%
!!! TTACCTTTTTTTTTTGTGTT+GGG - chr2.2:68337127-68337146 MS.gene01482:intron 20.0%
!!! TTGATGATTTTTGATACATA+GGG - chr2.2:68335849-68335868 MS.gene01482:CDS 20.0%
!!! TTTGATGATTTTTGATACAT+AGG - chr2.2:68335848-68335867 MS.gene01482:CDS 20.0%
!!! TTTTCATAAGTTAGTAGAAA+GGG + chr2.2:68336692-68336711 None:intergenic 20.0%
! AAAATAATCAACAAAGGAAG+TGG - chr2.2:68338147-68338166 MS.gene01482:intron 25.0%
! AACTATTATTCGCTTATCAA+TGG - chr2.2:68338323-68338342 MS.gene01482:CDS 25.0%
! AATTTGTAAAGGAACTCATA+TGG + chr2.2:68337471-68337490 None:intergenic 25.0%
! ACAACTAATTATACCAAACA+TGG - chr2.2:68338284-68338303 MS.gene01482:CDS 25.0%
! ATGTTTGGTATAATTAGTTG+TGG + chr2.2:68338285-68338304 None:intergenic 25.0%
! CATTGCATGTGTTAATTATT+GGG - chr2.2:68336135-68336154 MS.gene01482:intron 25.0%
! GTACCAAAAGAAATAGAATT+GGG + chr2.2:68337220-68337239 None:intergenic 25.0%
! GTTACAGTAACAAATTACTT+AGG + chr2.2:68337042-68337061 None:intergenic 25.0%
! TGAATCTCTTACATTGTTAA+TGG + chr2.2:68338121-68338140 None:intergenic 25.0%
! TGAATTACTAATAAGAGAGA+AGG + chr2.2:68337366-68337385 None:intergenic 25.0%
! TGTACCAAAAGAAATAGAAT+TGG + chr2.2:68337221-68337240 None:intergenic 25.0%
! TTTGATCTTAAATCTTGCTA+AGG - chr2.2:68335679-68335698 MS.gene01482:CDS 25.0%
!!! AATTGGTTTTCCAAAACTTA+AGG + chr2.2:68337109-68337128 None:intergenic 25.0%
!!! ACTAGAGCAATTTTTTCTAA+TGG - chr2.2:68337913-68337932 MS.gene01482:CDS 25.0%
!!! GACACTTATTTTAAAAGAGA+CGG - chr2.2:68337712-68337731 MS.gene01482:intron 25.0%
!!! TCAAATTGCTATTGATGTTT+TGG + chr2.2:68337012-68337031 None:intergenic 25.0%
AAAAATCAGGTACTAATCAG+TGG - chr2.2:68335633-68335652 MS.gene01482:CDS 30.0%
AAAACCAGAGAAGACTAATT+GGG - chr2.2:68337993-68338012 MS.gene01482:CDS 30.0%
AAACCAATGAATGCTTGAAT+TGG - chr2.2:68336877-68336896 MS.gene01482:intron 30.0%
AAGAAGGAATTGAAACAAGA+TGG - chr2.2:68337875-68337894 MS.gene01482:CDS 30.0%
AATTATGACCATATGCATGT+TGG - chr2.2:68338240-68338259 MS.gene01482:intron 30.0%
ATAGTGCACATTGCAAAAAA+TGG - chr2.2:68336465-68336484 MS.gene01482:intron 30.0%
ATATGTGTGTGTGTGTATAT+TGG - chr2.2:68337292-68337311 MS.gene01482:intron 30.0%
ATGAATGAAGAAGAAAGAGA+TGG - chr2.2:68338039-68338058 MS.gene01482:intron 30.0%
ATTGTTACCTAAAATGTTGC+AGG - chr2.2:68337757-68337776 MS.gene01482:intron 30.0%
CATCCCAACACAAAAAAAAA+AGG + chr2.2:68337133-68337152 None:intergenic 30.0%
CTAGTACTTCTACATCATTT+GGG + chr2.2:68335920-68335939 None:intergenic 30.0%
GAAGGGATTTAAGAAGATAT+TGG - chr2.2:68337942-68337961 MS.gene01482:CDS 30.0%
GCATTGCATGTGTTAATTAT+TGG - chr2.2:68336134-68336153 MS.gene01482:intron 30.0%
TAACAGTCAAAATTGTGCAT+TGG + chr2.2:68336303-68336322 None:intergenic 30.0%
TCTAGTACTTCTACATCATT+TGG + chr2.2:68335921-68335940 None:intergenic 30.0%
TGGTGGAAATAGAGTAATTA+AGG + chr2.2:68337403-68337422 None:intergenic 30.0%
TGTGAATTGTGGAAAAAACA+TGG - chr2.2:68336768-68336787 MS.gene01482:intron 30.0%
TGTGTGTATATTGGTGAATT+GGG - chr2.2:68337301-68337320 MS.gene01482:intron 30.0%
! ATGATTTTTGATACATAGGG+TGG - chr2.2:68335852-68335871 MS.gene01482:CDS 30.0%
! TTTTCCTTCTTTGCAATAGT+TGG + chr2.2:68337977-68337996 None:intergenic 30.0%
!! CTTGGACATGTATTTTGAAA+CGG + chr2.2:68336262-68336281 None:intergenic 30.0%
!! TCACCCAATTCTATTTCTTT+TGG - chr2.2:68337214-68337233 MS.gene01482:intron 30.0%
!!! GACATGTATTTTGAAACGAA+GGG - chr2.2:68336487-68336506 MS.gene01482:intron 30.0%
!!! TTTTCTAATGGTGTTGTGAA+GGG - chr2.2:68337925-68337944 MS.gene01482:CDS 30.0%
!!! TTTTTCTAATGGTGTTGTGA+AGG - chr2.2:68337924-68337943 MS.gene01482:CDS 30.0%
AAAAACCTGGTAATTGTGCT+GGG + chr2.2:68336046-68336065 None:intergenic 35.0%
AAACAAGATGGCACAAAACT+GGG - chr2.2:68337887-68337906 MS.gene01482:CDS 35.0%
AACACATGCAATGCATCAAA+CGG + chr2.2:68336128-68336147 None:intergenic 35.0%
AGGAACAAGGAAAAGAAGAA+AGG - chr2.2:68337840-68337859 MS.gene01482:CDS 35.0%
ATGGAATTACCATGTTGTCT+AGG + chr2.2:68338102-68338121 None:intergenic 35.0%
ATTAAGAGCTACATTGTTGG+AGG + chr2.2:68338193-68338212 None:intergenic 35.0%
CAACAATGTAGCTCTTAATG+TGG - chr2.2:68338194-68338213 MS.gene01482:intron 35.0%
CACATTAAGAGCTACATTGT+TGG + chr2.2:68338196-68338215 None:intergenic 35.0%
CTTCTTCATTCATACCAAGA+TGG + chr2.2:68338032-68338051 None:intergenic 35.0%
GAAAACCAGAGAAGACTAAT+TGG - chr2.2:68337992-68338011 MS.gene01482:CDS 35.0%
GCATAACACTATGATGAACA+TGG + chr2.2:68337423-68337442 None:intergenic 35.0%
GGTATGAACTCATCAATAAG+TGG + chr2.2:68335987-68336006 None:intergenic 35.0%
GTGTGTGTATATTGGTGAAT+TGG - chr2.2:68337300-68337319 MS.gene01482:intron 35.0%
GTTCATCATAGTGTTATGCA+TGG - chr2.2:68337424-68337443 MS.gene01482:intron 35.0%
TAAAAACCTGGTAATTGTGC+TGG + chr2.2:68336047-68336066 None:intergenic 35.0%
TAACACTATGATGAACATGG+TGG + chr2.2:68337420-68337439 None:intergenic 35.0%
TACACCAACTATTGCAAAGA+AGG - chr2.2:68337970-68337989 MS.gene01482:CDS 35.0%
TGGAAGGTCTATGAAAAAAG+TGG + chr2.2:68337807-68337826 None:intergenic 35.0%
TTGCCAATTCAAGCATTCAT+TGG + chr2.2:68336883-68336902 None:intergenic 35.0%
TTGTCGTAGACAAAAATGTG+CGG - chr2.2:68337590-68337609 MS.gene01482:intron 35.0%
TTTCTTTGCTTGCAATTGCA+AGG + chr2.2:68336636-68336655 None:intergenic 35.0%
! CTCAAGCTCTGGTAATTAAA+AGG - chr2.2:68335707-68335726 MS.gene01482:CDS 35.0%
! TAGAGAGCATAAATGTTCTG+TGG + chr2.2:68336585-68336604 None:intergenic 35.0%
! TCCTAGAGTGTGTAATTTTC+AGG - chr2.2:68336813-68336832 MS.gene01482:intron 35.0%
! TGCAAACAAAGTAAGAGTAC+TGG - chr2.2:68338350-68338369 MS.gene01482:CDS 35.0%
!! AAAATTACACACTCTAGGAC+TGG + chr2.2:68336812-68336831 None:intergenic 35.0%
!! TGTAGAAGTACTAGAGCATT+TGG - chr2.2:68335926-68335945 MS.gene01482:CDS 35.0%
!!! GACATGTATTTTGAAACGGA+GGG + chr2.2:68336258-68336277 None:intergenic 35.0%
!!! GGACATGTATTTTGAAACGA+AGG - chr2.2:68336486-68336505 MS.gene01482:intron 35.0%
AACATGGCTGTTCAAAGTGA+TGG - chr2.2:68338300-68338319 MS.gene01482:CDS 40.0%
AAGCATACACAACAGGAACA+AGG - chr2.2:68337827-68337846 MS.gene01482:intron 40.0%
AAGGTTCACACTGATGAAGA+AGG - chr2.2:68337859-68337878 MS.gene01482:CDS 40.0%
ATATGCATGTTGGTCATAGC+AGG - chr2.2:68338250-68338269 MS.gene01482:intron 40.0%
ATGACCACCAAACTCTCAAT+AGG - chr2.2:68336907-68336926 MS.gene01482:intron 40.0%
GAAAAAGCCCCCATCAAAAT+AGG + chr2.2:68336938-68336957 None:intergenic 40.0%
GAAACAAGATGGCACAAAAC+TGG - chr2.2:68337886-68337905 MS.gene01482:CDS 40.0%
GCAATTGCAAGGACTAAGTA+AGG + chr2.2:68336625-68336644 None:intergenic 40.0%
GCCTGAAAATTACACACTCT+AGG + chr2.2:68336817-68336836 None:intergenic 40.0%
GTGATGCATCAATACCATCT+TGG - chr2.2:68338015-68338034 MS.gene01482:intron 40.0%
TAATTACCAGAGCTTGAGAC+TGG + chr2.2:68335705-68335724 None:intergenic 40.0%
TATGCATGTTGGTCATAGCA+GGG - chr2.2:68338251-68338270 MS.gene01482:intron 40.0%
TCAATATTATGGCCGCATGA+GGG + chr2.2:68335609-68335628 None:intergenic 40.0%
TCACTTTGAACAGCCATGTT+TGG + chr2.2:68338300-68338319 None:intergenic 40.0%
TGACCACCAAACTCTCAATA+GGG - chr2.2:68336908-68336927 MS.gene01482:intron 40.0%
TGCTATGACCAACATGCATA+TGG + chr2.2:68338251-68338270 None:intergenic 40.0%
TGTAACACACTTCAACGAGT+TGG + chr2.2:68336855-68336874 None:intergenic 40.0%
TGTTCCTGTTGTGTATGCTT+TGG + chr2.2:68337827-68337846 None:intergenic 40.0%
TGTTGGGATGTTTACACTCT+TGG - chr2.2:68337143-68337162 MS.gene01482:intron 40.0%
TTCAATATTATGGCCGCATG+AGG + chr2.2:68335610-68335629 None:intergenic 40.0%
! GCTAACTCCTGCAACATTTT+AGG + chr2.2:68337767-68337786 None:intergenic 40.0%
!! AGAAGTACTAGAGCATTTGG+AGG - chr2.2:68335929-68335948 MS.gene01482:CDS 40.0%
!! ATCACCCAATTAGTCTTCTC+TGG + chr2.2:68338000-68338019 None:intergenic 40.0%
!!! ATAGGGGCATCCTATTTTGA+TGG - chr2.2:68336925-68336944 MS.gene01482:intron 40.0%
!!! GGACATGTATTTTGAAACGG+AGG + chr2.2:68336259-68336278 None:intergenic 40.0%
!!! TAGGGGCATCCTATTTTGAT+GGG - chr2.2:68336926-68336945 MS.gene01482:intron 40.0%
AGACACGATCTTGTTCTGCA+CGG - chr2.2:68336158-68336177 MS.gene01482:intron 45.0%
AGGCTGCACTGTTATTACCA+TGG + chr2.2:68338173-68338192 None:intergenic 45.0%
ATCAACAAAGGAAGTGGCCA+TGG - chr2.2:68338153-68338172 MS.gene01482:intron 45.0%
CAATATTATGGCCGCATGAG+GGG + chr2.2:68335608-68335627 None:intergenic 45.0%
CATGTGTGAAGTGGTTGACT+TGG + chr2.2:68336280-68336299 None:intergenic 45.0%
CCAACGCTTCAAGGAGATAA+TGG - chr2.2:68336005-68336024 MS.gene01482:CDS 45.0%
CCATTATCTCCTTGAAGCGT+TGG + chr2.2:68336008-68336027 None:intergenic 45.0%
CCTTCCAAAGCATACACAAC+AGG - chr2.2:68337820-68337839 MS.gene01482:intron 45.0%
GACCACCAAACTCTCAATAG+GGG - chr2.2:68336909-68336928 MS.gene01482:intron 45.0%
GAGTTCATACCAACGCTTCA+AGG - chr2.2:68335996-68336015 MS.gene01482:CDS 45.0%
GGGTGGAGTTAATGAATTGC+CGG - chr2.2:68335869-68335888 MS.gene01482:CDS 45.0%
GGTGGAGTTAATGAATTGCC+GGG - chr2.2:68335870-68335889 MS.gene01482:CDS 45.0%
GTGGAGTTAATGAATTGCCG+GGG - chr2.2:68335871-68335890 MS.gene01482:CDS 45.0%
GTTGCAGGAGTTAGCAAAGA+TGG - chr2.2:68337772-68337791 MS.gene01482:intron 45.0%
TTGTCGAATCTCTACGACTC+TGG - chr2.2:68335748-68335767 MS.gene01482:CDS 45.0%
! CCTGTTGTGTATGCTTTGGA+AGG + chr2.2:68337823-68337842 None:intergenic 45.0%
!!! AGGGGCATCCTATTTTGATG+GGG - chr2.2:68336927-68336946 MS.gene01482:intron 45.0%
ACACACCCAGCACAATTACC+AGG - chr2.2:68336038-68336057 MS.gene01482:CDS 50.0%
CAGACACAGCCTAGACAACA+TGG - chr2.2:68338090-68338109 MS.gene01482:intron 50.0%
GGATGCCCCTATTGAGAGTT+TGG + chr2.2:68336917-68336936 None:intergenic 50.0%
GGGTCAAATTTCACTCCAGC+TGG + chr2.2:68335900-68335919 None:intergenic 50.0%
TCCATTGAATGCCCCTCATG+CGG - chr2.2:68335594-68335613 MS.gene01482:CDS 50.0%
TGCCCCTATTGAGAGTTTGG+TGG + chr2.2:68336914-68336933 None:intergenic 50.0%
! GTGCATTGGCATGTGTGAAG+TGG + chr2.2:68336289-68336308 None:intergenic 50.0%
!!! GGGGCATCCTATTTTGATGG+GGG - chr2.2:68336928-68336947 MS.gene01482:intron 50.0%
CTAAGGCCAGTCTCAAGCTC+TGG - chr2.2:68335696-68335715 MS.gene01482:CDS 55.0%
TTCACTCCAGCTGGTAACCC+CGG + chr2.2:68335891-68335910 None:intergenic 55.0%
GAATTGCCGGGGTTACCAGC+TGG - chr2.2:68335882-68335901 MS.gene01482:CDS 60.0%
!! GCCGCATGAGGGGCATTCAA+TGG + chr2.2:68335598-68335617 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 68335510 68338383 68335510 ID=MS.gene01482
chr2.2 mRNA 68335510 68338383 68335510 ID=MS.gene01482.t1;Parent=MS.gene01482
chr2.2 exon 68338252 68338383 68338252 ID=MS.gene01482.t1.exon1;Parent=MS.gene01482.t1
chr2.2 CDS 68338252 68338383 68338252 ID=cds.MS.gene01482.t1;Parent=MS.gene01482.t1
chr2.2 exon 68337834 68338023 68337834 ID=MS.gene01482.t1.exon2;Parent=MS.gene01482.t1
chr2.2 CDS 68337834 68338023 68337834 ID=cds.MS.gene01482.t1;Parent=MS.gene01482.t1
chr2.2 exon 68335510 68336114 68335510 ID=MS.gene01482.t1.exon3;Parent=MS.gene01482.t1
chr2.2 CDS 68335510 68336114 68335510 ID=cds.MS.gene01482.t1;Parent=MS.gene01482.t1
Gene Sequence

>MS.gene01482

ATGGCATGGTGCAATGATAAAGATAACAACAACTCTGATGTCACTGATAACATAAAGCTCAATGAAGAAATTGATCAAAATCAATCCATTGAATGCCCCTCATGCGGCCATAATATTGAAGTAAAAAATCAGGTACTAATCAGTGGTTTTATTTGTTTTACTTATTTAGTTTGATCTTAAATCTTGCTAAGGCCAGTCTCAAGCTCTGGTAATTAAAAGGACTCAACAACATAAAACTTTGTCGAATCTCTACGACTCTGGTTAAATTTGATGTTAGTTTTATTTTGTTTGTTTATGCACCGATATTAATTCCTTTAGTTTATTAATTTGTGTGTAAATTTGATGATTTTTGATACATAGGGTGGAGTTAATGAATTGCCGGGGTTACCAGCTGGAGTGAAATTTGACCCAAATGATGTAGAAGTACTAGAGCATTTGGAGGCAAAAGTGATGTCTGATGTTCGCAAGATTCATCCACTTATTGATGAGTTCATACCAACGCTTCAAGGAGATAATGGAATTTGTTATACACACCCAGCACAATTACCAGGTTTTTATATTTAATTTAATGTATTTGATTTTTGTTTAATTAAAATGATTTAGAGATTTGGAATACCGTTTGATGCATTGCATGTGTTAATTATTGGGAGACACGATCTTGTTCTGCACGGCACCCCATGCATCAAATTAATTAATTAATTAATGTTTTATAATTTTTTTTTTTTTGGTGAATTAATGTTTTACTCCCTCCGTTTCAAAATACATGTCCAAGTCAACCACTTCACACATGCCAATGCACAATTTTGACTGTTAATATCTTTAATTATCTAAAGTAAAAAATTATAAAAATTTAATATTTTGAAAATATTTGTCGAGACAAATCAAACAATATCTTACATGCTAATATTTGTATTTATAGATTAGTTAAAAAATATGGTCAAAGTAAGTCAAGTGAATAGTGCACATTGCAAAAAATGGACATGTATTTTGAAACGAAGGGAGTATAATTTAAAGATTATATAGTTTTTTTTTAATTGAAATAGATTTTAATTATAAGGCGGTTTGAGCATGACCACAGAACATTTATGCTCTCTACATTTATAGCAGTAGTTCCTTACTTAGTCCTTGCAATTGCAAGCAAAGAAAATTATTTGTCAATTGGGGTTGAGTTGAGTCTAGCCCTTTCTACTAACTTATGAAAATTAAATAAATTAATGTTCAGATCTTAATCATTTAGCGTTTGAACATGATTATTTTTTGTGAATTGTGGAAAAAACATGGTAAAAAGAAAGTTAATGTCCAGTCCTAGAGTGTGTAATTTTCAGGCATCTCTTTCATATAACCAACTCGTTGAAGTGTGTTACAAGAAACCAATGAATGCTTGAATTGGCAATGCAATGACCACCAAACTCTCAATAGGGGCATCCTATTTTGATGGGGGCTTTTTCAACGTCTTACTGTATGATATAACTAATAAGCAGATTTTTTTAGATTTAACTCCAAAACATCAATAGCAATTTGAAATTGAGCCTAAGTAATTTGTTACTGTAACATTAGGTTAATTGTTAATGAAGTTAAAGACAATTAAAAATTGTTCCTTAAGTTTTGGAAAACCAATTACCTTTTTTTTTTGTGTTGGGATGTTTACACTCTTGGTTTGACACAGATTGAGTCTAATAAAAACTCCTTTTGATTAATAATGTATCACCCAATTCTATTTCTTTTGGTACATTGCAAAGAACAAACAAATAGCAATTTATCAGTATATTGATATATAAATATATATGTGTGTGTGTGTATATTGGTGAATTGGGTATATTAGTAATTAAGAGACAAATAAATTTTGCTTTTTTCCTTCTCTCTTATTAGTAATTCATTTTTAGTACTATTCCTTAATTACTCTATTTCCACCATGTTCATCATAGTGTTATGCATGGTTCACTGATTCTCATATTTAACCATATGAGTTCCTTTACAAATTCTTATTCACTTCAAAAATAATTGTCGTTTTTTTTTGACACAATAATTGTCGTTAATGTCATAGTTTAAATAAATAAAAGAATATTGCATTTTTACAAAATTGTCGTAGACAAAAATGTGCGGAGAAATATATTGACTTGAAAACGGTGTAGACAATATTAATTTAGAAATAGATTAGAAAAATAATATGTAATTAATATTACATTGAGAAAAAGGTAAAACGACACTTATTTTAAAAGAGACGGAATATGCAAGAAGTTGATATAAATTGTTACCTAAAATGTTGCAGGAGTTAGCAAAGATGGTCAGATTCGCCACTTTTTTCATAGACCTTCCAAAGCATACACAACAGGAACAAGGAAAAGAAGAAAGGTTCACACTGATGAAGAAGGAATTGAAACAAGATGGCACAAAACTGGGAAAACTAGAGCAATTTTTTCTAATGGTGTTGTGAAGGGATTTAAGAAGATATTGGTACTCTACACCAACTATTGCAAAGAAGGAAAACCAGAGAAGACTAATTGGGTGATGCATCAATACCATCTTGGTATGAATGAAGAAGAAAGAGATGGAGAATTTGTTGCTTCAAAGATCTTCTATCAGACACAGCCTAGACAACATGGTAATTCCATTAACAATGTAAGAGATTCATATGAAAAAATAATCAACAAAGGAAGTGGCCATGGTAATAACAGTGCAGCCTCCAACAATGTAGCTCTTAATGTGGAATGCTACAATGCTTGTTATACAAATTATGACCATATGCATGTTGGTCATAGCAGGGAAAGTACTCCACAACTAATTATACCAAACATGGCTGTTCAAAGTGATGGAACTATTATTCGCTTATCAATGGATCATGCAAACAAAGTAAGAGTACTGGAGAGAAAGTAA
Protein sequence

>MS.gene01482.t1

MAWCNDKDNNNSDVTDNIKLNEEIDQNQSIECPSCGHNIEVKNQGGVNELPGLPAGVKFDPNDVEVLEHLEAKVMSDVRKIHPLIDEFIPTLQGDNGICYTHPAQLPGVSKDGQIRHFFHRPSKAYTTGTRKRRKVHTDEEGIETRWHKTGKTRAIFSNGVVKGFKKILVLYTNYCKEGKPEKTNWVMHQYHLGMNEEERDGEFVASKIFYQTQPRQHGNSINNVRDSYEKIINKGSGHGNNSAASNNVALNVECYNACYTNYDHMHVGHSRESTPQLIIPNMAVQSDGTIIRLSMDHANKVRVLERK