Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014888.t1 | XP_003603313.1 | 86.8 | 235 | 24 | 3 | 1 | 235 | 1 | 228 | 1.30E-106 | 396 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014888.t1 | Q39262 | 36.6 | 246 | 107 | 13 | 29 | 252 | 17 | 235 | 6.9e-19 | 95.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014888.t1 | G7J9W4 | 86.8 | 235 | 24 | 3 | 1 | 235 | 1 | 228 | 9.6e-107 | 396.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene014888.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014888.t1 | MTR_3g106230 | 85.532 | 235 | 27 | 3 | 1 | 235 | 1 | 228 | 2.59e-140 | 393 |
| MS.gene014888.t1 | MTR_1g013130 | 48.438 | 256 | 107 | 11 | 5 | 252 | 24 | 262 | 2.70e-60 | 191 |
| MS.gene014888.t1 | MTR_2g104900 | 38.583 | 254 | 117 | 15 | 12 | 252 | 20 | 247 | 4.17e-32 | 118 |
| MS.gene014888.t1 | MTR_4g006575 | 37.037 | 189 | 79 | 6 | 75 | 252 | 65 | 224 | 2.07e-25 | 100 |
| MS.gene014888.t1 | MTR_4g006600 | 37.037 | 189 | 79 | 6 | 75 | 252 | 65 | 224 | 2.07e-25 | 100 |
| MS.gene014888.t1 | MTR_1g033340 | 44.068 | 118 | 42 | 6 | 54 | 165 | 69 | 168 | 7.60e-19 | 83.2 |
| MS.gene014888.t1 | MTR_5g077370 | 50.538 | 93 | 26 | 3 | 76 | 167 | 86 | 159 | 4.64e-18 | 80.9 |
| MS.gene014888.t1 | MTR_3g065020 | 48.913 | 92 | 26 | 4 | 77 | 167 | 86 | 157 | 3.33e-17 | 78.6 |
| MS.gene014888.t1 | MTR_1g107055 | 41.584 | 101 | 29 | 4 | 71 | 166 | 57 | 132 | 2.34e-14 | 69.7 |
| MS.gene014888.t1 | MTR_4g128340 | 73.684 | 38 | 10 | 0 | 75 | 112 | 43 | 80 | 7.33e-14 | 67.8 |
| MS.gene014888.t1 | MTR_3g011970 | 41.837 | 98 | 48 | 4 | 69 | 166 | 90 | 178 | 1.49e-13 | 69.3 |
| MS.gene014888.t1 | MTR_8g466760 | 35.075 | 134 | 65 | 6 | 51 | 184 | 75 | 186 | 2.09e-12 | 65.9 |
| MS.gene014888.t1 | MTR_3g009410 | 35.398 | 113 | 52 | 4 | 74 | 176 | 95 | 196 | 2.88e-11 | 62.8 |
| MS.gene014888.t1 | MTR_3g011990 | 36.364 | 110 | 50 | 4 | 71 | 176 | 92 | 185 | 5.15e-11 | 62.0 |
| MS.gene014888.t1 | MTR_5g008280 | 34.454 | 119 | 62 | 4 | 77 | 195 | 46 | 148 | 5.93e-11 | 62.0 |
| MS.gene014888.t1 | MTR_7g022980 | 35.088 | 114 | 56 | 5 | 75 | 184 | 64 | 163 | 7.32e-11 | 61.2 |
| MS.gene014888.t1 | MTR_7g086860 | 61.538 | 39 | 15 | 0 | 77 | 115 | 30 | 68 | 9.94e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene014888.t1 | AT5G25160 | 38.496 | 226 | 91 | 12 | 48 | 252 | 37 | 235 | 4.93e-28 | 107 |
| MS.gene014888.t1 | AT5G10970 | 36.652 | 221 | 97 | 10 | 48 | 252 | 79 | 272 | 4.63e-26 | 103 |
| MS.gene014888.t1 | AT1G80730 | 49.485 | 97 | 46 | 3 | 71 | 166 | 60 | 154 | 6.55e-22 | 90.9 |
| MS.gene014888.t1 | AT1G66140 | 46.739 | 92 | 37 | 3 | 77 | 161 | 83 | 169 | 6.51e-16 | 75.5 |
| MS.gene014888.t1 | AT1G24625 | 76.316 | 38 | 9 | 0 | 74 | 111 | 54 | 91 | 3.43e-15 | 72.4 |
| MS.gene014888.t1 | AT5G57520 | 65.909 | 44 | 15 | 0 | 75 | 118 | 48 | 91 | 1.84e-14 | 69.3 |
| MS.gene014888.t1 | AT5G14010 | 73.529 | 34 | 9 | 0 | 77 | 110 | 36 | 69 | 2.02e-12 | 63.9 |
| MS.gene014888.t1 | AT1G67030 | 44.944 | 89 | 44 | 3 | 44 | 132 | 7 | 90 | 6.47e-11 | 60.5 |
| MS.gene014888.t1 | AT3G58070 | 52.459 | 61 | 24 | 1 | 56 | 116 | 73 | 128 | 7.33e-11 | 61.2 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAATTGAAAGCAATTATTA+TGG | 0.211258 | 3.3:+87184921 | MS.gene014888:CDS |
| TATGAGACCATTTGCAATTA+AGG | 0.217801 | 3.3:+87184848 | MS.gene014888:CDS |
| TCACAACCGTTATGCAAATA+TGG | 0.314680 | 3.3:+87184788 | MS.gene014888:CDS |
| TAGATTCGGAAAGGTTAAAA+AGG | 0.319744 | 3.3:+87185049 | MS.gene014888:CDS |
| GCTACTGATGCAAACTTTCC+AGG | 0.343879 | 3.3:+87184995 | MS.gene014888:CDS |
| CCTAACGATGATTGTGATCT+TGG | 0.347148 | 3.3:+87184498 | MS.gene014888:CDS |
| TTCCAGATTGTTGATTCATA+AGG | 0.348814 | 3.3:-87184965 | None:intergenic |
| TCCAGATTGTTGATTCATAA+GGG | 0.359823 | 3.3:-87184964 | None:intergenic |
| ATTGCAGAATCCACATGAAT+TGG | 0.369569 | 3.3:+87184407 | MS.gene014888:CDS |
| GAATTGAAAGCAATTATTAT+GGG | 0.373275 | 3.3:+87184922 | MS.gene014888:CDS |
| AGAATTATCTATGTCTAAAC+AGG | 0.387114 | 3.3:-87184547 | None:intergenic |
| ATGCAAACTTTCCAGGAAAC+AGG | 0.389355 | 3.3:+87185002 | MS.gene014888:CDS |
| GTGATGGTAATCCTGTTTCC+TGG | 0.396510 | 3.3:-87185013 | None:intergenic |
| TTGGATGAAAATTTATGAAA+AGG | 0.404264 | 3.3:-87184891 | None:intergenic |
| CAAAGTAGTGTTGGTAGATT+CGG | 0.411274 | 3.3:+87185035 | MS.gene014888:CDS |
| AGCAATTATTATGGGTATCA+TGG | 0.430203 | 3.3:+87184930 | MS.gene014888:CDS |
| CCCCTTATGAATCAACAATC+TGG | 0.432563 | 3.3:+87184963 | MS.gene014888:CDS |
| GACATGCTCAATTCAGGAAC+TGG | 0.435799 | 3.3:+87185071 | MS.gene014888:CDS |
| AGAACGATGTTCTCAGCAAC+AGG | 0.459446 | 3.3:+87184732 | MS.gene014888:CDS |
| TTCTTCAGTTCACAAGCACT+TGG | 0.470251 | 3.3:+87184657 | MS.gene014888:CDS |
| CTAACGATGATTGTGATCTT+GGG | 0.483992 | 3.3:+87184499 | MS.gene014888:CDS |
| GCTTTCAATTCCAAAGCCAT+TGG | 0.496119 | 3.3:-87184910 | None:intergenic |
| TCTTCAGTTCACAAGCACTT+GGG | 0.508835 | 3.3:+87184658 | MS.gene014888:CDS |
| CTTCAGTTCACAAGCACTTG+GGG | 0.514360 | 3.3:+87184659 | MS.gene014888:CDS |
| GAGTGTGCCTTAATTGCAAA+TGG | 0.520506 | 3.3:-87184855 | None:intergenic |
| TGCAATTAAGGCACACTCGA+TGG | 0.523345 | 3.3:+87184860 | MS.gene014888:CDS |
| CCAAGATCACAATCATCGTT+AGG | 0.527605 | 3.3:-87184498 | None:intergenic |
| GGAAGAAGAAAAGGAAGCAA+AGG | 0.530366 | 3.3:+87184449 | MS.gene014888:CDS |
| ATGAATCAACAATCTGGAAG+AGG | 0.552126 | 3.3:+87184969 | MS.gene014888:CDS |
| ATGATATTTATGAAGAAATG+AGG | 0.554191 | 3.3:-87184763 | None:intergenic |
| GAGATGCCATATTTGCATAA+CGG | 0.557005 | 3.3:-87184794 | None:intergenic |
| ATTATTATGGGTATCATGGA+TGG | 0.573211 | 3.3:+87184934 | MS.gene014888:CDS |
| TTATGTTTCTCCAATTCATG+TGG | 0.577273 | 3.3:-87184417 | None:intergenic |
| CCAGATTGTTGATTCATAAG+GGG | 0.580761 | 3.3:-87184963 | None:intergenic |
| TAGTGTTGGTAGATTCGGAA+AGG | 0.588233 | 3.3:+87185040 | MS.gene014888:CDS |
| CTCGAAGAGTAATCAACAAG+AGG | 0.595525 | 3.3:+87185100 | MS.gene014888:CDS |
| CTACCAACACTACTTTGTGA+TGG | 0.596720 | 3.3:-87185029 | None:intergenic |
| TTACCATCACAAAGTAGTGT+TGG | 0.606864 | 3.3:+87185026 | MS.gene014888:CDS |
| TCATCAGAAAACTTCACTTG+TGG | 0.611490 | 3.3:+87184579 | MS.gene014888:CDS |
| AGAGCTTTAACGATAAGTCA+AGG | 0.617739 | 3.3:-87185130 | None:intergenic |
| AAAATGCTCACAAGAGAGAG+AGG | 0.643637 | 3.3:+87184688 | MS.gene014888:CDS |
| TTCAGTTCACAAGCACTTGG+GGG | 0.644584 | 3.3:+87184660 | MS.gene014888:CDS |
| ATATTTATGAAGAAATGAGG+TGG | 0.662714 | 3.3:-87184760 | None:intergenic |
| TTACTATTAGCACAGAAGAG+TGG | 0.684083 | 3.3:-87184822 | None:intergenic |
| ACGATGTTCTCAGCAACAGG+AGG | 0.689396 | 3.3:+87184735 | MS.gene014888:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGATATTTATGAAGAAATG+AGG | - | chr3.3:87184766-87184785 | None:intergenic | 20.0% |
| !! | GAATTGAAAGCAATTATTAT+GGG | + | chr3.3:87184922-87184941 | MS.gene014888:CDS | 20.0% |
| !! | TTGGATGAAAATTTATGAAA+AGG | - | chr3.3:87184894-87184913 | None:intergenic | 20.0% |
| !!! | TTTCATAAATTTTCATCCAA+TGG | + | chr3.3:87184894-87184913 | MS.gene014888:CDS | 20.0% |
| ! | AGAATTATCTATGTCTAAAC+AGG | - | chr3.3:87184550-87184569 | None:intergenic | 25.0% |
| ! | ATATTTATGAAGAAATGAGG+TGG | - | chr3.3:87184763-87184782 | None:intergenic | 25.0% |
| ! | GGAATTGAAAGCAATTATTA+TGG | + | chr3.3:87184921-87184940 | MS.gene014888:CDS | 25.0% |
| AGCAATTATTATGGGTATCA+TGG | + | chr3.3:87184930-87184949 | MS.gene014888:CDS | 30.0% | |
| ATTATTATGGGTATCATGGA+TGG | + | chr3.3:87184934-87184953 | MS.gene014888:CDS | 30.0% | |
| TAGATTCGGAAAGGTTAAAA+AGG | + | chr3.3:87185049-87185068 | MS.gene014888:CDS | 30.0% | |
| TATGAGACCATTTGCAATTA+AGG | + | chr3.3:87184848-87184867 | MS.gene014888:CDS | 30.0% | |
| TTATGTTTCTCCAATTCATG+TGG | - | chr3.3:87184420-87184439 | None:intergenic | 30.0% | |
| ! | AAATTTTCATCCAATGGCTT+TGG | + | chr3.3:87184900-87184919 | MS.gene014888:CDS | 30.0% |
| ! | GGAGAAACATAAAACTTTTG+AGG | + | chr3.3:87184428-87184447 | MS.gene014888:CDS | 30.0% |
| !! | AACTTTTGAGGAAGAAGAAA+AGG | + | chr3.3:87184440-87184459 | MS.gene014888:CDS | 30.0% |
| !! | TCCAGATTGTTGATTCATAA+GGG | - | chr3.3:87184967-87184986 | None:intergenic | 30.0% |
| !! | TTCCAGATTGTTGATTCATA+AGG | - | chr3.3:87184968-87184987 | None:intergenic | 30.0% |
| AAAAAGGACATGCTCAATTC+AGG | + | chr3.3:87185065-87185084 | MS.gene014888:CDS | 35.0% | |
| ATGAATCAACAATCTGGAAG+AGG | + | chr3.3:87184969-87184988 | MS.gene014888:CDS | 35.0% | |
| ATTGCAGAATCCACATGAAT+TGG | + | chr3.3:87184407-87184426 | MS.gene014888:CDS | 35.0% | |
| CTAACGATGATTGTGATCTT+GGG | + | chr3.3:87184499-87184518 | MS.gene014888:CDS | 35.0% | |
| GAGATGCCATATTTGCATAA+CGG | - | chr3.3:87184797-87184816 | None:intergenic | 35.0% | |
| TAGTTGCAAGTGAAAAATCG+TGG | - | chr3.3:87184624-87184643 | None:intergenic | 35.0% | |
| TCACAACCGTTATGCAAATA+TGG | + | chr3.3:87184788-87184807 | MS.gene014888:CDS | 35.0% | |
| TCATCAGAAAACTTCACTTG+TGG | + | chr3.3:87184579-87184598 | MS.gene014888:CDS | 35.0% | |
| TTACCATCACAAAGTAGTGT+TGG | + | chr3.3:87185026-87185045 | MS.gene014888:CDS | 35.0% | |
| TTACTATTAGCACAGAAGAG+TGG | - | chr3.3:87184825-87184844 | None:intergenic | 35.0% | |
| ! | AGAGCTTTAACGATAAGTCA+AGG | - | chr3.3:87185133-87185152 | None:intergenic | 35.0% |
| !! | CAAAGTAGTGTTGGTAGATT+CGG | + | chr3.3:87185035-87185054 | MS.gene014888:CDS | 35.0% |
| !! | CCAGATTGTTGATTCATAAG+GGG | - | chr3.3:87184966-87184985 | None:intergenic | 35.0% |
| AAAATGCTCACAAGAGAGAG+AGG | + | chr3.3:87184688-87184707 | MS.gene014888:CDS | 40.0% | |
| ATGCAAACTTTCCAGGAAAC+AGG | + | chr3.3:87185002-87185021 | MS.gene014888:CDS | 40.0% | |
| CCAAGATCACAATCATCGTT+AGG | - | chr3.3:87184501-87184520 | None:intergenic | 40.0% | |
| CCCCTTATGAATCAACAATC+TGG | + | chr3.3:87184963-87184982 | MS.gene014888:CDS | 40.0% | |
| CCTAACGATGATTGTGATCT+TGG | + | chr3.3:87184498-87184517 | MS.gene014888:CDS | 40.0% | |
| CTACCAACACTACTTTGTGA+TGG | - | chr3.3:87185032-87185051 | None:intergenic | 40.0% | |
| CTCGAAGAGTAATCAACAAG+AGG | + | chr3.3:87185100-87185119 | MS.gene014888:CDS | 40.0% | |
| GAGAGGTCAATTGCAAAAAG+AGG | + | chr3.3:87184705-87184724 | MS.gene014888:CDS | 40.0% | |
| GAGTGTGCCTTAATTGCAAA+TGG | - | chr3.3:87184858-87184877 | None:intergenic | 40.0% | |
| GCTTTCAATTCCAAAGCCAT+TGG | - | chr3.3:87184913-87184932 | None:intergenic | 40.0% | |
| GGAAGAAGAAAAGGAAGCAA+AGG | + | chr3.3:87184449-87184468 | MS.gene014888:CDS | 40.0% | |
| GGTCAATTGCAAAAAGAGGA+CGG | + | chr3.3:87184709-87184728 | MS.gene014888:CDS | 40.0% | |
| TCTTCAGTTCACAAGCACTT+GGG | + | chr3.3:87184658-87184677 | MS.gene014888:CDS | 40.0% | |
| TTCTTCAGTTCACAAGCACT+TGG | + | chr3.3:87184657-87184676 | MS.gene014888:CDS | 40.0% | |
| !! | TAGTGTTGGTAGATTCGGAA+AGG | + | chr3.3:87185040-87185059 | MS.gene014888:CDS | 40.0% |
| AGAACGATGTTCTCAGCAAC+AGG | + | chr3.3:87184732-87184751 | MS.gene014888:CDS | 45.0% | |
| CTTCAGTTCACAAGCACTTG+GGG | + | chr3.3:87184659-87184678 | MS.gene014888:CDS | 45.0% | |
| GACATGCTCAATTCAGGAAC+TGG | + | chr3.3:87185071-87185090 | MS.gene014888:CDS | 45.0% | |
| GCTACTGATGCAAACTTTCC+AGG | + | chr3.3:87184995-87185014 | MS.gene014888:CDS | 45.0% | |
| GTGATGGTAATCCTGTTTCC+TGG | - | chr3.3:87185016-87185035 | None:intergenic | 45.0% | |
| TGCAATTAAGGCACACTCGA+TGG | + | chr3.3:87184860-87184879 | MS.gene014888:CDS | 45.0% | |
| TTCAGTTCACAAGCACTTGG+GGG | + | chr3.3:87184660-87184679 | MS.gene014888:CDS | 45.0% | |
| ACGATGTTCTCAGCAACAGG+AGG | + | chr3.3:87184735-87184754 | MS.gene014888:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 87184396 | 87185154 | 87184396 | ID=MS.gene014888 |
| chr3.3 | mRNA | 87184396 | 87185154 | 87184396 | ID=MS.gene014888.t1;Parent=MS.gene014888 |
| chr3.3 | exon | 87184396 | 87185154 | 87184396 | ID=MS.gene014888.t1.exon1;Parent=MS.gene014888.t1 |
| chr3.3 | CDS | 87184396 | 87185154 | 87184396 | ID=cds.MS.gene014888.t1;Parent=MS.gene014888.t1 |
| Gene Sequence |
| Protein sequence |
