AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015023


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015023.t1 MTR_3g104370 92.169 166 13 0 1 166 65 230 9.41e-111 315
MS.gene015023.t1 MTR_4g100630 54.082 98 41 2 18 114 86 180 6.06e-29 108
MS.gene015023.t1 MTR_6g092540 58.621 87 32 2 24 109 94 177 7.86e-27 102
MS.gene015023.t1 MTR_8g101650 61.333 75 26 1 24 98 102 173 4.78e-26 100
MS.gene015023.t1 MTR_2g100930 66.176 68 20 1 24 91 106 170 3.13e-25 99.8
MS.gene015023.t1 MTR_5g069710 49.091 110 43 3 24 133 129 225 4.26e-25 98.6
MS.gene015023.t1 MTR_7g067080 60.759 79 27 1 11 85 120 198 5.27e-24 95.9
MS.gene015023.t1 MTR_3g462790 67.742 62 20 0 24 85 144 205 1.83e-23 94.4
MS.gene015023.t1 MTR_4g107230 71.698 53 15 0 21 73 78 130 2.15e-23 93.6
MS.gene015023.t1 MTR_5g081860 60.759 79 25 3 6 80 102 178 2.59e-23 93.2
MS.gene015023.t1 MTR_5g037080 72.222 54 15 0 21 74 96 149 3.15e-23 93.6
MS.gene015023.t1 MTR_1g111830 69.841 63 17 1 24 86 121 181 5.16e-23 92.0
MS.gene015023.t1 MTR_4g111975 57.746 71 27 1 24 94 135 202 1.19e-22 92.0
MS.gene015023.t1 MTR_5g088010 50.000 72 36 0 21 92 54 125 1.35e-20 85.5
MS.gene015023.t1 MTR_1g048660 65.385 52 18 0 22 73 7 58 1.08e-19 79.3
MS.gene015023.t1 MTR_2g090305 59.322 59 24 0 27 85 28 86 1.58e-19 81.6
MS.gene015023.t1 MTR_5g027570 51.389 72 34 1 21 92 86 156 3.82e-19 81.3
MS.gene015023.t1 MTR_0036s0260 59.322 59 24 0 27 85 28 86 4.47e-19 80.5
MS.gene015023.t1 MTR_5g088060 54.688 64 29 0 29 92 274 337 8.26e-19 82.8
MS.gene015023.t1 MTR_5g488160 54.688 64 29 0 29 92 274 337 8.26e-19 82.8
MS.gene015023.t1 MTR_8g063600 51.948 77 36 1 18 94 97 172 3.61e-18 79.7
MS.gene015023.t1 MTR_5g027550 59.259 54 22 0 21 74 71 124 6.38e-17 75.5
MS.gene015023.t1 MTR_4g015130 62.745 51 19 0 35 85 1 51 4.51e-16 69.7
MS.gene015023.t1 MTR_8g063870 50.000 68 32 1 29 94 94 161 5.80e-15 70.9
MS.gene015023.t1 MTR_1g083180 45.205 73 39 1 21 93 55 126 5.53e-14 66.6
MS.gene015023.t1 MTR_3g070110 48.571 70 32 1 29 94 97 166 8.88e-13 63.9
MS.gene015023.t1 MTR_5g075790 64.583 48 16 1 29 76 115 161 1.25e-12 63.9
MS.gene015023.t1 MTR_5g075760 64.583 48 16 1 29 76 107 153 1.44e-12 63.5
MS.gene015023.t1 MTR_1g033620 54.902 51 20 1 42 92 20 67 6.24e-12 62.0
MS.gene015023.t1 MTR_7g110040 70.732 41 11 1 40 80 11 50 8.92e-12 57.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015023.t1 AT5G56840 47.794 136 67 2 24 159 88 219 4.57e-28 104
MS.gene015023.t1 AT5G47390 54.255 94 31 2 18 111 87 168 4.38e-27 104
MS.gene015023.t1 AT5G58900 54.545 88 39 1 24 111 138 224 3.45e-24 95.9
MS.gene015023.t1 AT5G61620 52.222 90 40 1 8 97 89 175 4.28e-24 96.3
MS.gene015023.t1 AT2G38090 67.742 62 20 0 24 85 138 199 6.48e-24 95.5
MS.gene015023.t1 AT1G70000 74.074 54 14 0 21 74 92 145 8.46e-24 94.4
MS.gene015023.t1 AT1G70000 74.074 54 14 0 21 74 92 145 8.46e-24 94.4
MS.gene015023.t1 AT5G01200 66.129 62 21 0 24 85 143 204 1.01e-23 94.4
MS.gene015023.t1 AT1G49010 60.563 71 25 2 24 94 131 198 1.15e-22 92.4
MS.gene015023.t1 AT3G16350 72.549 51 14 0 24 74 134 184 1.87e-22 92.8
MS.gene015023.t1 AT3G11280 66.129 62 21 0 24 85 124 185 1.21e-21 88.6
MS.gene015023.t1 AT3G11280 66.129 62 21 0 24 85 124 185 1.21e-21 88.6
MS.gene015023.t1 AT5G04760 65.079 63 20 1 24 86 96 156 1.43e-21 87.4
MS.gene015023.t1 AT5G08520 57.746 71 27 2 24 94 115 182 1.51e-21 89.0
MS.gene015023.t1 AT1G74840 70.000 50 15 0 24 73 93 142 3.29e-21 87.0
MS.gene015023.t1 AT1G74840 70.000 50 15 0 24 73 93 142 4.62e-21 87.0
MS.gene015023.t1 AT5G05790 64.516 62 22 0 24 85 128 189 5.46e-21 87.0
MS.gene015023.t1 AT5G05790 64.516 62 22 0 24 85 128 189 5.46e-21 87.0
MS.gene015023.t1 AT1G19000 70.000 50 15 0 24 73 99 148 9.49e-21 86.7
MS.gene015023.t1 AT1G19000 70.000 50 15 0 24 73 99 148 9.49e-21 86.7
MS.gene015023.t1 AT3G10580 55.072 69 26 1 24 92 92 155 2.48e-20 85.1
MS.gene015023.t1 AT3G10580 55.072 69 26 1 24 92 92 155 3.94e-20 85.1
MS.gene015023.t1 AT4G09450 51.220 82 37 2 24 103 88 168 8.70e-19 79.7
MS.gene015023.t1 AT5G23650 62.000 50 19 0 24 73 117 166 2.67e-14 69.7
MS.gene015023.t1 AT3G10590 53.448 58 27 0 28 85 112 169 8.01e-13 63.9

Find 39 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGAGTCAAATTTATTTC+TGG 0.179125 3.2:-86202845 None:intergenic
TCCTCCTTGCCTACCATGTT+TGG 0.292024 3.2:+86202127 None:intergenic
CTTAGAGGACTTGAGAAGAT+TGG 0.328823 3.2:+86202558 MS.gene015023:CDS
AGAAGATTGGGAAGGGTAAC+TGG 0.333260 3.2:+86202571 MS.gene015023:CDS
CTTATGCAGCTGATATCATT+TGG 0.348494 3.2:-86202863 None:intergenic
TTTGTGCATGACTAGCTACC+TGG 0.362976 3.2:-86202635 None:intergenic
AATTTCTACTTCCACTTAGT+TGG 0.375503 3.2:-86202886 None:intergenic
CTCAACATTCAATACTGAAA+TGG 0.409371 3.2:+86202933 MS.gene015023:CDS
TTAGAGGACTTGAGAAGATT+GGG 0.415766 3.2:+86202559 MS.gene015023:CDS
TCCGTTGATGTTGAAGAGTT+AGG 0.422454 3.2:-86202956 None:intergenic
GCAAGTTTGAGTTCTAAATC+AGG 0.432705 3.2:-86203001 None:intergenic
GAGCACATGATATTTCTTAG+AGG 0.438938 3.2:+86202543 MS.gene015023:CDS
TGATATATGCTCGTTTCTTA+CGG 0.451802 3.2:-86202173 None:intergenic
CATGCCTCTGTTAGCTCCAA+TGG 0.472733 3.2:-86203034 None:intergenic
GGACTTGAGAAGATTGGGAA+GGG 0.480087 3.2:+86202564 MS.gene015023:CDS
CTCTGTTAGCTCCAATGGCA+TGG 0.483024 3.2:-86203029 None:intergenic
AGGACTTGAGAAGATTGGGA+AGG 0.495301 3.2:+86202563 MS.gene015023:CDS
TTACATAATGGAGGCTAAGA+TGG 0.500282 3.2:-86202707 None:intergenic
ACTTGCAACTCCCATGCCAT+TGG 0.501098 3.2:+86203018 MS.gene015023:CDS
GTTGAGGATGAGAAAGCCAC+TGG 0.522676 3.2:-86202916 None:intergenic
AAATCAGGTGCTGCACTATG+AGG 0.523156 3.2:-86202986 None:intergenic
TCTGTTAGCTCCAATGGCAT+GGG 0.536107 3.2:-86203028 None:intergenic
TGATCAAGCTATTCTGAGAC+TGG 0.538315 3.2:-86202671 None:intergenic
ACACTACTGGTTAGCTTAAG+GGG 0.538405 3.2:-86203059 None:intergenic
TTGTGCATGACTAGCTACCT+GGG 0.547274 3.2:-86202634 None:intergenic
TTAATTTACTTACATAATGG+AGG 0.564586 3.2:-86202716 None:intergenic
ATGTGCTCCTCCTCGGTCCA+CGG 0.572063 3.2:-86202528 None:intergenic
GCCTAACTCTTCAACATCAA+CGG 0.576928 3.2:+86202955 MS.gene015023:CDS
CATTTCAGTATTGAATGTTG+AGG 0.583001 3.2:-86202932 None:intergenic
AGCTGCATAAGCCAACTAAG+TGG 0.584599 3.2:+86202875 MS.gene015023:CDS
AAATATCATGTGCTCCTCCT+CGG 0.585344 3.2:-86202535 None:intergenic
TTGAGGATGAGAAAGCCACT+GGG 0.600136 3.2:-86202915 None:intergenic
ATCAGGTATGCCGTGGACCG+AGG 0.606863 3.2:+86202518 MS.gene015023:intron
GCATGACTAGCTACCTGGGT+TGG 0.613754 3.2:-86202630 None:intergenic
ATTGGGAAGGGTAACTGGAG+AGG 0.616689 3.2:+86202576 MS.gene015023:CDS
AGGTATGCCGTGGACCGAGG+AGG 0.665092 3.2:+86202521 MS.gene015023:intron
TCAAGCTATTCTGAGACTGG+CGG 0.679211 3.2:-86202668 None:intergenic
CATGCCATTGGAGCTAACAG+AGG 0.690851 3.2:+86203030 MS.gene015023:CDS
TGAGGATGAGAAAGCCACTG+GGG 0.761973 3.2:-86202914 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTATTCTATATTTAATT+TGG + chr3.2:86202804-86202823 MS.gene015023:intron 10.0%
!!! ATTTTAATTTACTTACATAA+TGG - chr3.2:86202722-86202741 None:intergenic 10.0%
!!! AATTAAAGAAGTTTAAAGTA+AGG - chr3.2:86202778-86202797 None:intergenic 15.0%
!!! ATAATTATAACCTTTTTTAC+TGG - chr3.2:86202209-86202228 None:intergenic 15.0%
!!! TAATTATAACCTTTTTTACT+GGG - chr3.2:86202208-86202227 None:intergenic 15.0%
!! TTAATTTACTTACATAATGG+AGG - chr3.2:86202719-86202738 None:intergenic 20.0%
!!! TATTCTATATTTAATTTGGC+AGG + chr3.2:86202808-86202827 MS.gene015023:intron 20.0%
! TCCAACTAATGAGAAAATTA+AGG + chr3.2:86202323-86202342 MS.gene015023:intron 25.0%
! TGAATCCAAAATTCAAAATC+TGG + chr3.2:86202283-86202302 MS.gene015023:intron 25.0%
!! TCCTTAATTTTCTCATTAGT+TGG - chr3.2:86202327-86202346 None:intergenic 25.0%
!! TTTGGAGTCAAATTTATTTC+TGG - chr3.2:86202848-86202867 None:intergenic 25.0%
!!! ATGTACATTTTTTTGTGATC+AGG + chr3.2:86202501-86202520 MS.gene015023:intron 25.0%
!!! TGCACGTAACTATTTTTATA+TGG - chr3.2:86202751-86202770 None:intergenic 25.0%
AATTTCTACTTCCACTTAGT+TGG - chr3.2:86202889-86202908 None:intergenic 30.0%
CTCAACATTCAATACTGAAA+TGG + chr3.2:86202933-86202952 MS.gene015023:CDS 30.0%
TAGAATTGTGAATTGTTGCT+TGG + chr3.2:86202462-86202481 MS.gene015023:intron 30.0%
TGATATATGCTCGTTTCTTA+CGG - chr3.2:86202176-86202195 None:intergenic 30.0%
! CATTTCAGTATTGAATGTTG+AGG - chr3.2:86202935-86202954 None:intergenic 30.0%
!! AGTTTTGTGAGGGTTAAATT+AGG + chr3.2:86202259-86202278 MS.gene015023:intron 30.0%
!! CTATATTTAATTTGGCAGGT+GGG + chr3.2:86202812-86202831 MS.gene015023:intron 30.0%
!! TCTATATTTAATTTGGCAGG+TGG + chr3.2:86202811-86202830 MS.gene015023:intron 30.0%
ACAATCAGACCCAGTAAAAA+AGG + chr3.2:86202196-86202215 MS.gene015023:CDS 35.0%
CTTATGCAGCTGATATCATT+TGG - chr3.2:86202866-86202885 None:intergenic 35.0%
GAGCACATGATATTTCTTAG+AGG + chr3.2:86202543-86202562 MS.gene015023:CDS 35.0%
TTAGAGGACTTGAGAAGATT+GGG + chr3.2:86202559-86202578 MS.gene015023:CDS 35.0%
! CTTGGCCAGATTTTGAATTT+TGG - chr3.2:86202291-86202310 None:intergenic 35.0%
! TTACATAATGGAGGCTAAGA+TGG - chr3.2:86202710-86202729 None:intergenic 35.0%
!! CTTACAGATCAGTTTTGTGA+GGG + chr3.2:86202249-86202268 MS.gene015023:intron 35.0%
!! GCAAGTTTGAGTTCTAAATC+AGG - chr3.2:86203004-86203023 None:intergenic 35.0%
AAATATCATGTGCTCCTCCT+CGG - chr3.2:86202538-86202557 None:intergenic 40.0%
AACAACAAAAACACCAACCC+AGG + chr3.2:86202617-86202636 MS.gene015023:CDS 40.0%
CCTCACAAAACTGATCTGTA+AGG - chr3.2:86202251-86202270 None:intergenic 40.0%
CTTAGAGGACTTGAGAAGAT+TGG + chr3.2:86202558-86202577 MS.gene015023:CDS 40.0%
GCCTAACTCTTCAACATCAA+CGG + chr3.2:86202955-86202974 MS.gene015023:CDS 40.0%
TCCGTTGATGTTGAAGAGTT+AGG - chr3.2:86202959-86202978 None:intergenic 40.0%
! GTAGAAATTTTCTTCCCCAG+TGG + chr3.2:86202900-86202919 MS.gene015023:CDS 40.0%
!! CCTTACAGATCAGTTTTGTG+AGG + chr3.2:86202248-86202267 MS.gene015023:intron 40.0%
!! TGATCAAGCTATTCTGAGAC+TGG - chr3.2:86202674-86202693 None:intergenic 40.0%
AGAAGATTGGGAAGGGTAAC+TGG + chr3.2:86202571-86202590 MS.gene015023:CDS 45.0%
AGCTGCATAAGCCAACTAAG+TGG + chr3.2:86202875-86202894 MS.gene015023:CDS 45.0%
AGGACTTGAGAAGATTGGGA+AGG + chr3.2:86202563-86202582 MS.gene015023:CDS 45.0%
GGACTTGAGAAGATTGGGAA+GGG + chr3.2:86202564-86202583 MS.gene015023:CDS 45.0%
TCTGTTAGCTCCAATGGCAT+GGG - chr3.2:86203031-86203050 None:intergenic 45.0%
TTGAGGATGAGAAAGCCACT+GGG - chr3.2:86202918-86202937 None:intergenic 45.0%
! AAATCAGGTGCTGCACTATG+AGG - chr3.2:86202989-86203008 None:intergenic 45.0%
! TTGTGCATGACTAGCTACCT+GGG - chr3.2:86202637-86202656 None:intergenic 45.0%
! TTTGTGCATGACTAGCTACC+TGG - chr3.2:86202638-86202657 None:intergenic 45.0%
! TTTTGTGATCAGGTATGCCG+TGG + chr3.2:86202511-86202530 MS.gene015023:intron 45.0%
!! TCAAGCTATTCTGAGACTGG+CGG - chr3.2:86202671-86202690 None:intergenic 45.0%
ACTTGCAACTCCCATGCCAT+TGG + chr3.2:86203018-86203037 MS.gene015023:CDS 50.0%
ATTGGGAAGGGTAACTGGAG+AGG + chr3.2:86202576-86202595 MS.gene015023:CDS 50.0%
CATGCCATTGGAGCTAACAG+AGG + chr3.2:86203030-86203049 MS.gene015023:CDS 50.0%
CATGCCTCTGTTAGCTCCAA+TGG - chr3.2:86203037-86203056 None:intergenic 50.0%
CTCTGTTAGCTCCAATGGCA+TGG - chr3.2:86203032-86203051 None:intergenic 50.0%
GTTGAGGATGAGAAAGCCAC+TGG - chr3.2:86202919-86202938 None:intergenic 50.0%
TGAGGATGAGAAAGCCACTG+GGG - chr3.2:86202917-86202936 None:intergenic 50.0%
! GTTGGAGCACTCACTTCACT+TGG - chr3.2:86202309-86202328 None:intergenic 50.0%
! GCATGACTAGCTACCTGGGT+TGG - chr3.2:86202633-86202652 None:intergenic 55.0%
ATCAGGTATGCCGTGGACCG+AGG + chr3.2:86202518-86202537 MS.gene015023:intron 60.0%
ATGTGCTCCTCCTCGGTCCA+CGG - chr3.2:86202531-86202550 None:intergenic 60.0%
AGGTATGCCGTGGACCGAGG+AGG + chr3.2:86202521-86202540 MS.gene015023:intron 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 86202142 86203066 86202142 ID=MS.gene015023
chr3.2 mRNA 86202142 86203066 86202142 ID=MS.gene015023.t1;Parent=MS.gene015023
chr3.2 exon 86202142 86202217 86202142 ID=MS.gene015023.t1.exon1;Parent=MS.gene015023.t1
chr3.2 CDS 86202142 86202217 86202142 ID=cds.MS.gene015023.t1;Parent=MS.gene015023.t1
chr3.2 exon 86202523 86202725 86202523 ID=MS.gene015023.t1.exon2;Parent=MS.gene015023.t1
chr3.2 CDS 86202523 86202725 86202523 ID=cds.MS.gene015023.t1;Parent=MS.gene015023.t1
chr3.2 exon 86202830 86203066 86202830 ID=MS.gene015023.t1.exon3;Parent=MS.gene015023.t1
chr3.2 CDS 86202830 86203066 86202830 ID=cds.MS.gene015023.t1;Parent=MS.gene015023.t1
Gene Sequence

>MS.gene015023

ATGTTTGGTTCAAATGAAAATTCAGATAGTTCCGTAAGAAACGAGCATATATCAACAATCAGACCCAGTAAAAAAGGTTATAATTATACTATTGTCTCATGTCTCACCTTACAGATCAGTTTTGTGAGGGTTAAATTAGGTTGAATCCAAAATTCAAAATCTGGCCAAGTGAAGTGAGTGCTCCAACTAATGAGAAAATTAAGGATTGACATTTCAACTAATTATGATTTAATTTGAACTATAATTTTAACAATTGATTTATTGATACTACTCACTTTACTCTAGTTATATGAATTTTTTTCTTTACATTTGCTATACTCTAGAATTGTGAATTGTTGCTTGGAAAAGAAATTAACATAATGTACATTTTTTTGTGATCAGGTATGCCGTGGACCGAGGAGGAGCACATGATATTTCTTAGAGGACTTGAGAAGATTGGGAAGGGTAACTGGAGAGGTATATCTAGAGATTTTGTAACAACAAAAACACCAACCCAGGTAGCTAGTCATGCACAAAAACATTTTCTCCGCCAGTCTCAGAATAGCTTGATCAATAGAAGAAAACACCATCTTAGCCTCCATTATGTAAGTAAATTAAAATATCTCTCCATATAAAAATAGTTACGTGCACAAACCTTACTTTAAACTTCTTTAATTTTCACTATCTTATTCTATATTTAATTTGGCAGGTGGGATGTGAAAAACCAGAAATAAATTTGACTCCAAATGATATCAGCTGCATAAGCCAACTAAGTGGAAGTAGAAATTTTCTTCCCCAGTGGCTTTCTCATCCTCAACATTCAATACTGAAATGGCCTAACTCTTCAACATCAACGGATTGTACTCCTCATAGTGCAGCACCTGATTTAGAACTCAAACTTGCAACTCCCATGCCATTGGAGCTAACAGAGGCATGCTCCCCTTAA

Protein sequence

>MS.gene015023.t1

MFGSNENSDSSVRNEHISTIRPSKKGMPWTEEEHMIFLRGLEKIGKGNWRGISRDFVTTKTPTQVASHAQKHFLRQSQNSLINRRKHHLSLHYVGCEKPEINLTPNDISCISQLSGSRNFLPQWLSHPQHSILKWPNSSTSTDCTPHSAAPDLELKLATPMPLELTEACSP