AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015078

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015078.t1 MTR_3g103110 63.164 809 138 8 1 658 1 800 0.0 947
MS.gene015078.t1 MTR_7g109640 71.160 638 138 3 44 658 182 796 0.0 873
MS.gene015078.t1 MTR_7g109640 66.250 80 24 1 22 101 74 150 7.33e-19 91.7
MS.gene015078.t1 MTR_3g103120 65.954 608 134 3 51 658 307 841 0.0 761
MS.gene015078.t1 MTR_3g103120 34.783 253 94 7 1 190 1 245 7.37e-21 98.2
MS.gene015078.t1 MTR_5g024390 72.955 440 101 5 223 658 3 428 0.0 665
MS.gene015078.t1 MTR_5g024500 72.500 440 103 5 223 658 3 428 0.0 659
MS.gene015078.t1 MTR_5g071340 40.826 436 225 8 222 651 678 1086 1.41e-105 346
MS.gene015078.t1 MTR_3g464550 41.149 435 221 9 222 649 602 1008 2.14e-104 342
MS.gene015078.t1 MTR_3g103160 40.100 601 189 16 57 651 3 438 3.73e-92 293
MS.gene015078.t1 MTR_2g078040 35.412 449 257 9 206 649 746 1166 3.27e-79 275
MS.gene015078.t1 MTR_1g103830 32.715 431 268 4 220 649 603 1012 2.00e-67 241
MS.gene015078.t1 MTR_1g072130 32.045 440 265 8 220 651 809 1222 3.27e-65 234
MS.gene015078.t1 MTR_7g095140 31.579 323 193 5 338 655 8 307 3.51e-49 176
MS.gene015078.t1 MTR_1g068930 26.013 469 250 15 224 649 441 855 6.69e-43 166
MS.gene015078.t1 MTR_1g068930 26.013 469 250 15 224 649 478 892 7.85e-43 166
MS.gene015078.t1 MTR_7g102610 25.263 475 237 14 249 656 527 950 9.31e-38 150
MS.gene015078.t1 MTR_4g099530 25.339 442 263 14 222 649 110 498 8.53e-34 136
MS.gene015078.t1 MTR_4g057290 26.744 430 206 12 257 621 126 511 1.22e-31 130
MS.gene015078.t1 MTR_4g056990 24.506 506 267 15 212 658 179 628 1.32e-30 127
MS.gene015078.t1 MTR_1g062330 24.837 459 241 12 257 651 386 804 1.69e-30 128
MS.gene015078.t1 MTR_4g050090 24.031 516 267 15 212 652 179 644 3.94e-28 120
MS.gene015078.t1 MTR_6g061000 58.140 86 27 2 310 395 25 101 3.11e-23 95.1
MS.gene015078.t1 MTR_2g084425 29.630 189 112 6 469 653 847 1018 3.17e-18 89.7
MS.gene015078.t1 MTR_3g109988 32.086 187 98 7 441 620 191 355 1.22e-14 76.3
MS.gene015078.t1 MTR_3g110005 32.086 187 98 7 441 620 191 355 1.22e-14 76.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015078.t1 AT5G58610 40.181 443 237 7 211 649 359 777 1.36e-101 330
MS.gene015078.t1 AT5G58610 40.181 443 237 7 211 649 590 1008 1.28e-99 328
MS.gene015078.t1 AT5G58610 40.181 443 237 7 211 649 590 1008 1.28e-99 328
MS.gene015078.t1 AT5G58610 40.181 443 237 7 211 649 590 1008 1.71e-99 328
MS.gene015078.t1 AT5G58610 40.181 443 237 7 211 649 581 999 3.07e-99 328
MS.gene015078.t1 AT5G58610 40.181 443 237 7 211 649 581 999 4.72e-99 327
MS.gene015078.t1 AT5G58610 38.095 441 223 7 211 649 581 973 7.02e-88 296
MS.gene015078.t1 AT5G58610 38.095 441 223 7 211 649 581 973 1.12e-87 296
MS.gene015078.t1 AT5G58610 39.642 391 208 7 211 597 590 956 2.76e-84 285
MS.gene015078.t1 AT5G58610 39.642 391 208 7 211 597 581 947 4.99e-84 284
MS.gene015078.t1 AT5G36740 32.054 443 260 8 215 649 540 949 6.93e-70 247
MS.gene015078.t1 AT5G36740 32.054 443 260 8 215 649 540 949 6.93e-70 247
MS.gene015078.t1 AT5G58610 38.244 353 190 7 211 559 581 909 9.98e-69 241
MS.gene015078.t1 AT1G05380 32.494 437 260 5 220 649 517 925 2.95e-68 242
MS.gene015078.t1 AT1G05380 32.494 437 260 5 220 649 517 925 2.95e-68 242
MS.gene015078.t1 AT1G05380 32.494 437 260 5 220 649 517 925 2.95e-68 242
MS.gene015078.t1 AT5G36740 31.473 448 266 8 215 649 387 806 1.34e-67 239
MS.gene015078.t1 AT3G14980 31.364 440 266 8 222 655 575 984 1.81e-64 231
MS.gene015078.t1 AT3G14980 31.364 440 266 8 222 655 620 1029 1.94e-64 231
MS.gene015078.t1 AT3G14980 31.364 440 266 8 222 655 620 1029 1.94e-64 231
MS.gene015078.t1 AT3G14980 31.364 440 266 8 222 655 620 1029 1.94e-64 231
MS.gene015078.t1 AT3G14980 31.364 440 266 8 222 655 620 1029 3.14e-64 230
MS.gene015078.t1 AT5G36670 29.847 459 263 10 215 649 540 963 4.76e-61 221
MS.gene015078.t1 AT5G36670 29.847 459 263 10 215 649 540 963 4.76e-61 221
MS.gene015078.t1 AT4G14920 30.155 451 283 6 208 652 562 986 1.00e-60 220
MS.gene015078.t1 AT4G14920 30.155 451 283 6 208 652 562 986 1.00e-60 220
MS.gene015078.t1 AT4G14920 30.155 451 283 6 208 652 562 986 1.09e-60 220
MS.gene015078.t1 AT5G36670 29.310 464 269 10 215 649 387 820 1.22e-58 214
MS.gene015078.t1 AT5G36740 31.429 385 223 8 215 591 540 891 1.76e-58 212
MS.gene015078.t1 AT5G36740 31.429 385 223 8 215 591 540 891 1.76e-58 212
MS.gene015078.t1 AT5G36670 31.429 385 223 8 215 591 540 891 1.76e-58 212
MS.gene015078.t1 AT5G36670 29.310 464 269 10 215 649 540 973 2.69e-58 213
MS.gene015078.t1 AT5G63900 36.570 309 169 8 346 651 258 542 4.89e-58 205
MS.gene015078.t1 AT3G53680 28.859 447 222 14 224 624 411 807 8.20e-44 169
MS.gene015078.t1 AT3G53680 28.859 447 222 14 224 624 409 805 9.35e-44 169
MS.gene015078.t1 AT3G14980 30.145 345 205 8 222 560 620 934 4.58e-42 164
MS.gene015078.t1 AT2G37520 26.025 488 239 15 224 651 392 817 5.61e-42 163
MS.gene015078.t1 AT2G27980 26.389 504 263 14 217 652 597 1060 2.73e-38 152
MS.gene015078.t1 AT2G36720 25.272 459 239 13 257 653 557 973 9.81e-38 151
MS.gene015078.t1 AT2G36720 25.272 459 239 13 257 653 557 973 9.81e-38 151

Find 118 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACATTGTTGTTTGCATTT+TGG 0.154123 3.2:-85543852 None:intergenic
GTTGCAACTATAAGAATTTA+TGG 0.208645 3.2:+85544335 MS.gene015078:CDS
AAGGCCATATAGGTCTATTT+AGG 0.215735 3.2:-85544616 None:intergenic
CCAACTATTGATGCTTTATC+TGG 0.251503 3.2:+85544203 MS.gene015078:CDS
AACACCATCATTATTTGCTT+TGG 0.281757 3.2:-85543688 None:intergenic
TTCTGTCTGAAGAAGCTATT+AGG 0.283860 3.2:+85544029 MS.gene015078:CDS
AGGTTGCAAATAAAATAAAT+TGG 0.285418 3.2:+85544063 MS.gene015078:CDS
TAGGTCTATTTAGGGATTTA+AGG 0.304954 3.2:-85544607 None:intergenic
GTTTGTTCTATTTGTGGTTT+GGG 0.311387 3.2:+85543708 MS.gene015078:CDS
GCTATTAGGGAAACCAATTA+AGG 0.314529 3.2:+85544043 MS.gene015078:CDS
AAAGGTATTCACTTGTTTCT+TGG 0.322354 3.2:+85543328 MS.gene015078:CDS
TGTGTAAAGGCTACAAGATT+TGG 0.323605 3.2:+85543936 MS.gene015078:CDS
GTTGATAAAGAATCAAGTTT+TGG 0.324712 3.2:+85543350 MS.gene015078:CDS
AGGCCATATAGGTCTATTTA+GGG 0.326281 3.2:-85544615 None:intergenic
AATCCCTAAATAGACCTATA+TGG 0.346948 3.2:+85544612 MS.gene015078:CDS
TGTTTGTTCTATTTGTGGTT+TGG 0.372212 3.2:+85543707 MS.gene015078:CDS
GAAAATGGGTAAAGTCATTT+CGG 0.376245 3.2:+85544313 MS.gene015078:CDS
TATGGCCTTGTATTGCTTGA+TGG 0.376913 3.2:+85544630 MS.gene015078:CDS
CTTAGGTCTCGATCAAGTTC+CGG 0.377173 3.2:+85543782 MS.gene015078:CDS
TTAGGTTCTGGGCAGTTCTC+AGG 0.378707 3.2:-85542712 None:intergenic
CGTGTTAATTTCACTATCAC+TGG 0.385095 3.2:+85543462 MS.gene015078:CDS
CCCTACTTCAGAACTTGCTT+CGG 0.386240 3.2:-85543652 None:intergenic
CCAGATAAAGCATCAATAGT+TGG 0.388520 3.2:-85544203 None:intergenic
AGTAACAATGTTGTCAAAAG+AGG 0.388998 3.2:+85543402 MS.gene015078:CDS
TCAGGTTCTATGTTGAAATC+AGG 0.401611 3.2:-85542694 None:intergenic
AGTATTAAAGATGAGTATAA+TGG 0.402129 3.2:+85543245 MS.gene015078:CDS
GATGGTGTTTGTTCTATTTG+TGG 0.402818 3.2:+85543702 MS.gene015078:CDS
CAAATTGACACTTGTCTTCA+CGG 0.405716 3.2:-85544544 None:intergenic
TTCTTCAATGCAACTCTCGC+AGG 0.405765 3.2:-85542767 None:intergenic
CAATTTCTTCGTCTCTGTTA+CGG 0.406401 3.2:+85542836 MS.gene015078:CDS
ACCATCCACTACTATCTTGT+TGG 0.407099 3.2:+85543518 MS.gene015078:CDS
TGCTATTGCTGCTGCTTCCT+CGG 0.411590 3.2:-85542947 None:intergenic
GCATTCCACCTCGTTTGCTT+AGG 0.414402 3.2:+85543765 MS.gene015078:CDS
GAGAAGAATTCAGAGGTTTA+CGG 0.421103 3.2:+85543267 MS.gene015078:CDS
GCTGCTTCCTCGGATTCTCT+TGG 0.429778 3.2:-85542937 None:intergenic
CCCACCGAATTAGTAACTTC+TGG 0.432390 3.2:-85543147 None:intergenic
TCTGTCTGAAGAAGCTATTA+GGG 0.432712 3.2:+85544030 MS.gene015078:CDS
TTTCACTATCACTGGATTTG+AGG 0.433458 3.2:+85543470 MS.gene015078:CDS
ATCAAGTTCCGGATGGTGAT+TGG 0.433575 3.2:+85543793 MS.gene015078:CDS
CATAAACGCTTGAATTTCCA+TGG 0.434704 3.2:+85544269 MS.gene015078:CDS
TATAAGAATTTATGGTCAAA+AGG 0.441015 3.2:+85544343 MS.gene015078:CDS
TCAGAGTTCGCATGATGAAA+AGG 0.443317 3.2:+85543578 MS.gene015078:CDS
CAGATAAAGCATCAATAGTT+GGG 0.446006 3.2:-85544202 None:intergenic
TGTTGCTTCTGCTGTTTCCT+CGG 0.450642 3.2:-85542881 None:intergenic
CAGAGTTCGCATGATGAAAA+GGG 0.457917 3.2:+85543579 MS.gene015078:CDS
GCTACTGGTGCTGCAGTCGA+AGG 0.458616 3.2:-85542811 None:intergenic
TGAACCAGAAGTTACTAATT+CGG 0.467506 3.2:+85543143 MS.gene015078:CDS
CAAGCAATACAAGGCCATAT+AGG 0.468400 3.2:-85544626 None:intergenic
CTGCAGTCGAAGGGTGTTTG+AGG 0.471352 3.2:-85542801 None:intergenic
GACGAAGAAATTGCTGCTAC+TGG 0.475004 3.2:-85542826 None:intergenic
GACACGTGGACAAAAGCGTT+TGG 0.477202 3.2:+85544506 MS.gene015078:CDS
GAGAAACAGTTGACACGCTT+AGG 0.478300 3.2:+85544443 MS.gene015078:CDS
AGGCTCATGCTGGTTGTACA+AGG 0.491245 3.2:+85543490 MS.gene015078:CDS
AAATTGACACTTGTCTTCAC+GGG 0.497364 3.2:-85544543 None:intergenic
GAGAGGGAAGGTATCAAAGA+GGG 0.502506 3.2:+85543293 MS.gene015078:CDS
TACTACGGTGTCAACATCAA+TGG 0.502626 3.2:+85543095 MS.gene015078:CDS
TTGTCTTCGATATTGTTCCT+TGG 0.503462 3.2:-85544390 None:intergenic
ATCATCAGAAGTTGATTCAT+CGG 0.513368 3.2:-85542983 None:intergenic
GGAAGACGGAAGGTCATTGT+TGG 0.514903 3.2:+85543539 MS.gene015078:CDS
ACTGGATTTGAGGCTCATGC+TGG 0.514944 3.2:+85543480 MS.gene015078:CDS
TATCAAAGAGGGAAGGTACA+AGG 0.524975 3.2:+85543304 MS.gene015078:CDS
CCACGTGTCTATAGCATCCT+CGG 0.528623 3.2:-85544492 None:intergenic
CCGAAGCAAGTTCTGAAGTA+GGG 0.528703 3.2:+85543652 MS.gene015078:CDS
TCTTCAATGCAACTCTCGCA+GGG 0.531694 3.2:-85542766 None:intergenic
GTTACTAATTCGGTGGGTAG+TGG 0.540215 3.2:+85543153 MS.gene015078:CDS
TGCAGTCGAAGGGTGTTTGA+GGG 0.545311 3.2:-85542800 None:intergenic
TCCGAAGCAAGTTCTGAAGT+AGG 0.548155 3.2:+85543651 MS.gene015078:CDS
ACGCTTAGGAGTTGAAAGCT+TGG 0.553379 3.2:+85544457 MS.gene015078:CDS
CAGACGATTGTGGTAGAAGA+GGG 0.555231 3.2:+85543220 MS.gene015078:CDS
CTACTGGTGCTGCAGTCGAA+GGG 0.556866 3.2:-85542810 None:intergenic
TGCGAGAGTTGCATTGAAGA+AGG 0.557070 3.2:+85542769 MS.gene015078:CDS
AACTACCATCAAGCAATACA+AGG 0.564139 3.2:-85544635 None:intergenic
GGACAAAACCAATCACCATC+CGG 0.566171 3.2:-85543801 None:intergenic
CAGAGGTTTACGGTAAGAGA+GGG 0.567218 3.2:+85543277 MS.gene015078:CDS
TCGGTGGGTAGTGGTAAAAG+AGG 0.571961 3.2:+85543162 MS.gene015078:CDS
AAATCACATTATAGAAACCA+TGG 0.572412 3.2:-85544286 None:intergenic
GGTAGCCACGAAAACTATCT+CGG 0.575564 3.2:-85544369 None:intergenic
TATAATGGAGAAGAATTCAG+AGG 0.575599 3.2:+85543260 MS.gene015078:CDS
TCAGACGATTGTGGTAGAAG+AGG 0.577488 3.2:+85543219 MS.gene015078:CDS
TCAGAGGTTTACGGTAAGAG+AGG 0.577597 3.2:+85543276 MS.gene015078:CDS
GTAACAATGTTGTCAAAAGA+GGG 0.581494 3.2:+85543403 MS.gene015078:CDS
TGTTCTATTTGTGGTTTGGG+AGG 0.585455 3.2:+85543711 MS.gene015078:CDS
ACTACTATCTTGTTGGAAGA+CGG 0.590421 3.2:+85543525 MS.gene015078:CDS
AAAGCGACCAAGAGAATCCG+AGG 0.592053 3.2:+85542930 MS.gene015078:CDS
AGAGAGGGAAGGTATCAAAG+AGG 0.595325 3.2:+85543292 MS.gene015078:CDS
GTTTCCAAAGCAAATAATGA+TGG 0.599744 3.2:+85543684 MS.gene015078:CDS
ACCAGAAGTTACTAATTCGG+TGG 0.600989 3.2:+85543146 MS.gene015078:CDS
AAAGAGATTGAGAGAATCCG+AGG 0.612293 3.2:+85542864 MS.gene015078:CDS
GAGAAGAATTCAGACGATTG+TGG 0.613172 3.2:+85543210 MS.gene015078:CDS
GTCATTGTTGGAATGTCAGA+GGG 0.614589 3.2:+85543551 MS.gene015078:CDS
AGAGGGAAGGTACAAGGGAA+AGG 0.618121 3.2:+85543310 MS.gene015078:CDS
GTCTTCCAACAAGATAGTAG+TGG 0.619064 3.2:-85543523 None:intergenic
GGTCATTGTTGGAATGTCAG+AGG 0.622266 3.2:+85543550 MS.gene015078:CDS
AAAGAAGAGAAAGTTGAATG+TGG 0.626130 3.2:+85544151 MS.gene015078:CDS
CCAGAAGTTACTAATTCGGT+GGG 0.626761 3.2:+85543147 MS.gene015078:CDS
TTCTGAATCAACTTCTGACG+AGG 0.630454 3.2:+85543029 MS.gene015078:CDS
TCCAACAAGATAGTAGTGGA+TGG 0.630549 3.2:-85543519 None:intergenic
CAAACGAGGTGGAATGCAGA+AGG 0.631169 3.2:-85543759 None:intergenic
GAGAGTTGCATTGAAGAAGG+AGG 0.635933 3.2:+85542772 MS.gene015078:CDS
GGGTAGTGGTAAAAGAGGAA+AGG 0.636646 3.2:+85543167 MS.gene015078:CDS
AGAGTTGCATTGAAGAAGGA+GGG 0.637310 3.2:+85542773 MS.gene015078:CDS
ATCAAAGAGGGAAGGTACAA+GGG 0.637578 3.2:+85543305 MS.gene015078:CDS
CTATCTTGTTGGAAGACGGA+AGG 0.639850 3.2:+85543529 MS.gene015078:CDS
GGGAAGGTATCAAAGAGGGA+AGG 0.651040 3.2:+85543297 MS.gene015078:CDS
AGAGTTCGCATGATGAAAAG+GGG 0.652963 3.2:+85543580 MS.gene015078:CDS
TCTATGTTGAAATCAGGAGG+TGG 0.653673 3.2:-85542688 None:intergenic
GGTTCTATGTTGAAATCAGG+AGG 0.654128 3.2:-85542691 None:intergenic
GGTTTACGGTAAGAGAGGGA+AGG 0.658054 3.2:+85543281 MS.gene015078:CDS
AAGATTTGGATCTAGTCACA+TGG 0.660288 3.2:+85543950 MS.gene015078:CDS
AATTTGAGTAGTGATGATGA+TGG 0.661624 3.2:+85544098 MS.gene015078:CDS
AAGGAACAATATCGAAGACA+AGG 0.663403 3.2:+85544392 MS.gene015078:CDS
CCGAGGATGCTATAGACACG+TGG 0.685477 3.2:+85544492 MS.gene015078:CDS
TTGAGTAGTGATGATGATGG+CGG 0.685672 3.2:+85544101 MS.gene015078:CDS
GGTCTCGATCAAGTTCCGGA+TGG 0.685979 3.2:+85543786 MS.gene015078:CDS
GATCGAGACCTAAGCAAACG+AGG 0.703690 3.2:-85543773 None:intergenic
CGAGACCTAAGCAAACGAGG+TGG 0.726299 3.2:-85543770 None:intergenic
CGTGTCTATAGCATCCTCGG+AGG 0.738184 3.2:-85544489 None:intergenic
TGAAAAGGGGAATCATTGTG+TGG 0.749018 3.2:+85543593 MS.gene015078:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGGTTGCAAATAAAATAAAT+TGG + chr3.2:85544063-85544082 MS.gene015078:CDS 20.0%
!! AGTATTAAAGATGAGTATAA+TGG + chr3.2:85543245-85543264 MS.gene015078:CDS 20.0%
!! TATAAGAATTTATGGTCAAA+AGG + chr3.2:85544343-85544362 MS.gene015078:CDS 20.0%
!!! AATGTGATTTTAGAGAAAAT+GGG + chr3.2:85544299-85544318 MS.gene015078:CDS 20.0%
!!! ATTTTATTTGCAACCTTAAT+TGG - chr3.2:85544059-85544078 None:intergenic 20.0%
!!! TAATGTGATTTTAGAGAAAA+TGG + chr3.2:85544298-85544317 MS.gene015078:CDS 20.0%
! AAATCACATTATAGAAACCA+TGG - chr3.2:85544289-85544308 None:intergenic 25.0%
! CAGTTCGATTAAAAAATCTA+CGG + chr3.2:85543071-85543090 MS.gene015078:CDS 25.0%
! GTTGCAACTATAAGAATTTA+TGG + chr3.2:85544335-85544354 MS.gene015078:CDS 25.0%
! TAAAAAATCTACGGTTACTA+CGG + chr3.2:85543080-85543099 MS.gene015078:CDS 25.0%
!! TCTAAGAGAAATGTAGTTTT+TGG - chr3.2:85542743-85542762 None:intergenic 25.0%
!!! GGTTTTAAAAATGAGTACAA+TGG + chr3.2:85543188-85543207 MS.gene015078:CDS 25.0%
!!! GTTGATAAAGAATCAAGTTT+TGG + chr3.2:85543350-85543369 MS.gene015078:CDS 25.0%
AAAGAAGAGAAAGTTGAATG+TGG + chr3.2:85544151-85544170 MS.gene015078:CDS 30.0%
AACACCATCATTATTTGCTT+TGG - chr3.2:85543691-85543710 None:intergenic 30.0%
AATCCCTAAATAGACCTATA+TGG + chr3.2:85544612-85544631 MS.gene015078:CDS 30.0%
AGTAACAATGTTGTCAAAAG+AGG + chr3.2:85543402-85543421 MS.gene015078:CDS 30.0%
GAAAATGGGTAAAGTCATTT+CGG + chr3.2:85544313-85544332 MS.gene015078:CDS 30.0%
GTAACAATGTTGTCAAAAGA+GGG + chr3.2:85543403-85543422 MS.gene015078:CDS 30.0%
GTTTCCAAAGCAAATAATGA+TGG + chr3.2:85543684-85543703 MS.gene015078:CDS 30.0%
GTTTGTTCTATTTGTGGTTT+GGG + chr3.2:85543708-85543727 MS.gene015078:CDS 30.0%
TAGGTCTATTTAGGGATTTA+AGG - chr3.2:85544610-85544629 None:intergenic 30.0%
TGAACCAGAAGTTACTAATT+CGG + chr3.2:85543143-85543162 MS.gene015078:CDS 30.0%
TGTTTGTTCTATTTGTGGTT+TGG + chr3.2:85543707-85543726 MS.gene015078:CDS 30.0%
! AATTTGAGTAGTGATGATGA+TGG + chr3.2:85544098-85544117 MS.gene015078:CDS 30.0%
! ATCATCAGAAGTTGATTCAT+CGG - chr3.2:85542986-85543005 None:intergenic 30.0%
! CAGATAAAGCATCAATAGTT+GGG - chr3.2:85544205-85544224 None:intergenic 30.0%
! CATATTTTGCAACAACAAGT+TGG - chr3.2:85543825-85543844 None:intergenic 30.0%
! TATAATGGAGAAGAATTCAG+AGG + chr3.2:85543260-85543279 MS.gene015078:CDS 30.0%
! TGTGAGCAAAATTTTCACTT+TGG + chr3.2:85543912-85543931 MS.gene015078:CDS 30.0%
! TTTTCACTTTGGATGTGTAA+AGG + chr3.2:85543923-85543942 MS.gene015078:CDS 30.0%
!! AAAGGTATTCACTTGTTTCT+TGG + chr3.2:85543328-85543347 MS.gene015078:CDS 30.0%
!!! AAAGATGTCGTTTTTAGTAG+AGG + chr3.2:85544239-85544258 MS.gene015078:CDS 30.0%
!!! AAGAATCAAGTTTTGGTTTC+TGG + chr3.2:85543357-85543376 MS.gene015078:CDS 30.0%
!!! AGAATCAAGTTTTGGTTTCT+GGG + chr3.2:85543358-85543377 MS.gene015078:CDS 30.0%
!!! AGAGGGAGTTTATTTTATGA+TGG + chr3.2:85543420-85543439 MS.gene015078:CDS 30.0%
!!! ATGTAGTTTTTGGCATTGTT+AGG - chr3.2:85542733-85542752 None:intergenic 30.0%
!!! CACATTGTTGTTTGCATTTT+GGG - chr3.2:85543854-85543873 None:intergenic 30.0%
AAATTGACACTTGTCTTCAC+GGG - chr3.2:85544546-85544565 None:intergenic 35.0%
AAGATTTGGATCTAGTCACA+TGG + chr3.2:85543950-85543969 MS.gene015078:CDS 35.0%
AAGGAACAATATCGAAGACA+AGG + chr3.2:85544392-85544411 MS.gene015078:CDS 35.0%
AAGGCCATATAGGTCTATTT+AGG - chr3.2:85544619-85544638 None:intergenic 35.0%
ACTACTATCTTGTTGGAAGA+CGG + chr3.2:85543525-85543544 MS.gene015078:CDS 35.0%
AGGCCATATAGGTCTATTTA+GGG - chr3.2:85544618-85544637 None:intergenic 35.0%
CAAATTGACACTTGTCTTCA+CGG - chr3.2:85544547-85544566 None:intergenic 35.0%
CAATTTCTTCGTCTCTGTTA+CGG + chr3.2:85542836-85542855 MS.gene015078:CDS 35.0%
CATAAACGCTTGAATTTCCA+TGG + chr3.2:85544269-85544288 MS.gene015078:CDS 35.0%
CCAACTATTGATGCTTTATC+TGG + chr3.2:85544203-85544222 MS.gene015078:CDS 35.0%
CGTGTTAATTTCACTATCAC+TGG + chr3.2:85543462-85543481 MS.gene015078:CDS 35.0%
GAGAAGAATTCAGAGGTTTA+CGG + chr3.2:85543267-85543286 MS.gene015078:CDS 35.0%
GATGGTGTTTGTTCTATTTG+TGG + chr3.2:85543702-85543721 MS.gene015078:CDS 35.0%
GCAATGTTAAAAACAGGAAC+TGG + chr3.2:85543976-85543995 MS.gene015078:CDS 35.0%
GCTATTAGGGAAACCAATTA+AGG + chr3.2:85544043-85544062 MS.gene015078:CDS 35.0%
TCAGGTTCTATGTTGAAATC+AGG - chr3.2:85542697-85542716 None:intergenic 35.0%
TCTGTCTGAAGAAGCTATTA+GGG + chr3.2:85544030-85544049 MS.gene015078:CDS 35.0%
TGGAGAGCAATGTTAAAAAC+AGG + chr3.2:85543970-85543989 MS.gene015078:CDS 35.0%
TGTGTAAAGGCTACAAGATT+TGG + chr3.2:85543936-85543955 MS.gene015078:CDS 35.0%
TTCTGTCTGAAGAAGCTATT+AGG + chr3.2:85544029-85544048 MS.gene015078:CDS 35.0%
TTGTCTTCGATATTGTTCCT+TGG - chr3.2:85544393-85544412 None:intergenic 35.0%
TTTCACTATCACTGGATTTG+AGG + chr3.2:85543470-85543489 MS.gene015078:CDS 35.0%
! CCAGATAAAGCATCAATAGT+TGG - chr3.2:85544206-85544225 None:intergenic 35.0%
! TTTTGGCATTGTTAGGTTCT+GGG - chr3.2:85542726-85542745 None:intergenic 35.0%
!! TTTTTGGCATTGTTAGGTTC+TGG - chr3.2:85542727-85542746 None:intergenic 35.0%
!!! GCACATTGTTGTTTGCATTT+TGG - chr3.2:85543855-85543874 None:intergenic 35.0%
AAAGAGATTGAGAGAATCCG+AGG + chr3.2:85542864-85542883 MS.gene015078:CDS 40.0%
ACCAGAAGTTACTAATTCGG+TGG + chr3.2:85543146-85543165 MS.gene015078:CDS 40.0%
ACCATCCACTACTATCTTGT+TGG + chr3.2:85543518-85543537 MS.gene015078:CDS 40.0%
AGAGTTCGCATGATGAAAAG+GGG + chr3.2:85543580-85543599 MS.gene015078:CDS 40.0%
AGAGTTGCATTGAAGAAGGA+GGG + chr3.2:85542773-85542792 MS.gene015078:CDS 40.0%
CAAGCAATACAAGGCCATAT+AGG - chr3.2:85544629-85544648 None:intergenic 40.0%
CAGAGTTCGCATGATGAAAA+GGG + chr3.2:85543579-85543598 MS.gene015078:CDS 40.0%
CCAGAAGTTACTAATTCGGT+GGG + chr3.2:85543147-85543166 MS.gene015078:CDS 40.0%
GAGAAGAATTCAGACGATTG+TGG + chr3.2:85543210-85543229 MS.gene015078:CDS 40.0%
GGTTCTATGTTGAAATCAGG+AGG - chr3.2:85542694-85542713 None:intergenic 40.0%
GTCATTGTTGGAATGTCAGA+GGG + chr3.2:85543551-85543570 MS.gene015078:CDS 40.0%
GTCTTCCAACAAGATAGTAG+TGG - chr3.2:85543526-85543545 None:intergenic 40.0%
TCAGAGTTCGCATGATGAAA+AGG + chr3.2:85543578-85543597 MS.gene015078:CDS 40.0%
TCTATGTTGAAATCAGGAGG+TGG - chr3.2:85542691-85542710 None:intergenic 40.0%
TGAAAAGGGGAATCATTGTG+TGG + chr3.2:85543593-85543612 MS.gene015078:CDS 40.0%
TTCTGAATCAACTTCTGACG+AGG + chr3.2:85543029-85543048 MS.gene015078:CDS 40.0%
! ATCAAAGAGGGAAGGTACAA+GGG + chr3.2:85543305-85543324 MS.gene015078:CDS 40.0%
! TACTACGGTGTCAACATCAA+TGG + chr3.2:85543095-85543114 MS.gene015078:CDS 40.0%
! TATCAAAGAGGGAAGGTACA+AGG + chr3.2:85543304-85543323 MS.gene015078:CDS 40.0%
! TCCAACAAGATAGTAGTGGA+TGG - chr3.2:85543522-85543541 None:intergenic 40.0%
! TGTTCTATTTGTGGTTTGGG+AGG + chr3.2:85543711-85543730 MS.gene015078:CDS 40.0%
!! TGGTTTTGCAGTGTAGTTTG+CGG + chr3.2:85543996-85544015 MS.gene015078:CDS 40.0%
!! TTGAGTAGTGATGATGATGG+CGG + chr3.2:85544101-85544120 MS.gene015078:CDS 40.0%
!!! CAAAAGCGTTTGGTTTTGCT+AGG + chr3.2:85544516-85544535 MS.gene015078:CDS 40.0%
ACGCTTAGGAGTTGAAAGCT+TGG + chr3.2:85544457-85544476 MS.gene015078:CDS 45.0%
ATCAAGTTCCGGATGGTGAT+TGG + chr3.2:85543793-85543812 MS.gene015078:CDS 45.0%
CAGACGATTGTGGTAGAAGA+GGG + chr3.2:85543220-85543239 MS.gene015078:CDS 45.0%
CAGAGGTTTACGGTAAGAGA+GGG + chr3.2:85543277-85543296 MS.gene015078:CDS 45.0%
CCCACCGAATTAGTAACTTC+TGG - chr3.2:85543150-85543169 None:intergenic 45.0%
CTATCTTGTTGGAAGACGGA+AGG + chr3.2:85543529-85543548 MS.gene015078:CDS 45.0%
CTTAGGTCTCGATCAAGTTC+CGG + chr3.2:85543782-85543801 MS.gene015078:CDS 45.0%
GACGAAGAAATTGCTGCTAC+TGG - chr3.2:85542829-85542848 None:intergenic 45.0%
GAGAAACAGTTGACACGCTT+AGG + chr3.2:85544443-85544462 MS.gene015078:CDS 45.0%
GAGAGTTGCATTGAAGAAGG+AGG + chr3.2:85542772-85542791 MS.gene015078:CDS 45.0%
GGACAAAACCAATCACCATC+CGG - chr3.2:85543804-85543823 None:intergenic 45.0%
GGTAGCCACGAAAACTATCT+CGG - chr3.2:85544372-85544391 None:intergenic 45.0%
GGTCATTGTTGGAATGTCAG+AGG + chr3.2:85543550-85543569 MS.gene015078:CDS 45.0%
TCAGACGATTGTGGTAGAAG+AGG + chr3.2:85543219-85543238 MS.gene015078:CDS 45.0%
TCAGAGGTTTACGGTAAGAG+AGG + chr3.2:85543276-85543295 MS.gene015078:CDS 45.0%
TCTTCAATGCAACTCTCGCA+GGG - chr3.2:85542769-85542788 None:intergenic 45.0%
TGCGAGAGTTGCATTGAAGA+AGG + chr3.2:85542769-85542788 MS.gene015078:CDS 45.0%
TTCTTCAATGCAACTCTCGC+AGG - chr3.2:85542770-85542789 None:intergenic 45.0%
! CCCTACTTCAGAACTTGCTT+CGG - chr3.2:85543655-85543674 None:intergenic 45.0%
! GATAGTTTTCGTGGCTACCA+AGG + chr3.2:85544373-85544392 MS.gene015078:CDS 45.0%
! GGGTAGTGGTAAAAGAGGAA+AGG + chr3.2:85543167-85543186 MS.gene015078:CDS 45.0%
! GTTACTAATTCGGTGGGTAG+TGG + chr3.2:85543153-85543172 MS.gene015078:CDS 45.0%
!! AGAGAGGGAAGGTATCAAAG+AGG + chr3.2:85543292-85543311 MS.gene015078:CDS 45.0%
!! CCGAAGCAAGTTCTGAAGTA+GGG + chr3.2:85543652-85543671 MS.gene015078:CDS 45.0%
!! GAGAGGGAAGGTATCAAAGA+GGG + chr3.2:85543293-85543312 MS.gene015078:CDS 45.0%
!! GGGACTACTGTGTTTTGTTG+TGG + chr3.2:85543378-85543397 MS.gene015078:CDS 45.0%
!! GGTTTTGCAGTGTAGTTTGC+GGG + chr3.2:85543997-85544016 MS.gene015078:CDS 45.0%
!! TCCGAAGCAAGTTCTGAAGT+AGG + chr3.2:85543651-85543670 MS.gene015078:CDS 45.0%
!! TGTTGCTTCTGCTGTTTCCT+CGG - chr3.2:85542884-85542903 None:intergenic 45.0%
AAAGCGACCAAGAGAATCCG+AGG + chr3.2:85542930-85542949 MS.gene015078:CDS 50.0%
CAAACGAGGTGGAATGCAGA+AGG - chr3.2:85543762-85543781 None:intergenic 50.0%
CCACGTGTCTATAGCATCCT+CGG - chr3.2:85544495-85544514 None:intergenic 50.0%
GATCGAGACCTAAGCAAACG+AGG - chr3.2:85543776-85543795 None:intergenic 50.0%
GCATTCCACCTCGTTTGCTT+AGG + chr3.2:85543765-85543784 MS.gene015078:CDS 50.0%
GGAAGACGGAAGGTCATTGT+TGG + chr3.2:85543539-85543558 MS.gene015078:CDS 50.0%
GGTTTACGGTAAGAGAGGGA+AGG + chr3.2:85543281-85543300 MS.gene015078:CDS 50.0%
! ACTGGATTTGAGGCTCATGC+TGG + chr3.2:85543480-85543499 MS.gene015078:CDS 50.0%
! AGAGGGAAGGTACAAGGGAA+AGG + chr3.2:85543310-85543329 MS.gene015078:CDS 50.0%
! AGGCTCATGCTGGTTGTACA+AGG + chr3.2:85543490-85543509 MS.gene015078:CDS 50.0%
! GGTTGCCGAGATAGTTTTCG+TGG + chr3.2:85544364-85544383 MS.gene015078:CDS 50.0%
! TCGGTGGGTAGTGGTAAAAG+AGG + chr3.2:85543162-85543181 MS.gene015078:CDS 50.0%
! TGCTATTGCTGCTGCTTCCT+CGG - chr3.2:85542950-85542969 None:intergenic 50.0%
!! GACACGTGGACAAAAGCGTT+TGG + chr3.2:85544506-85544525 MS.gene015078:CDS 50.0%
!! GGGAAGGTATCAAAGAGGGA+AGG + chr3.2:85543297-85543316 MS.gene015078:CDS 50.0%
!! TGCAGTCGAAGGGTGTTTGA+GGG - chr3.2:85542803-85542822 None:intergenic 50.0%
!! TTAGGTTCTGGGCAGTTCTC+AGG - chr3.2:85542715-85542734 None:intergenic 50.0%
!!! CTTGGTTTTGCATTCCTCCG+AGG + chr3.2:85544475-85544494 MS.gene015078:CDS 50.0%
CCGAGGATGCTATAGACACG+TGG + chr3.2:85544492-85544511 MS.gene015078:CDS 55.0%
CGAGACCTAAGCAAACGAGG+TGG - chr3.2:85543773-85543792 None:intergenic 55.0%
CGTGTCTATAGCATCCTCGG+AGG - chr3.2:85544492-85544511 None:intergenic 55.0%
GCTGCTTCCTCGGATTCTCT+TGG - chr3.2:85542940-85542959 None:intergenic 55.0%
GGTCTCGATCAAGTTCCGGA+TGG + chr3.2:85543786-85543805 MS.gene015078:CDS 55.0%
!! CTACTGGTGCTGCAGTCGAA+GGG - chr3.2:85542813-85542832 None:intergenic 55.0%
!! CTGCAGTCGAAGGGTGTTTG+AGG - chr3.2:85542804-85542823 None:intergenic 55.0%
!! GCTACTGGTGCTGCAGTCGA+AGG - chr3.2:85542814-85542833 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 85542673 85544649 85542673 ID=MS.gene015078
chr3.2 mRNA 85542673 85544649 85542673 ID=MS.gene015078.t1;Parent=MS.gene015078
chr3.2 exon 85542673 85544649 85542673 ID=MS.gene015078.t1.exon1;Parent=MS.gene015078.t1
chr3.2 CDS 85542673 85544649 85542673 ID=cds.MS.gene015078.t1;Parent=MS.gene015078.t1
Gene Sequence

>MS.gene015078

ATGGATTCATGTTCACCACCTCCTGATTTCAACATAGAACCTGAGAACTGCCCAGAACCTAACAATGCCAAAAACTACATTTCTCTTAGAAAACCCTGCGAGAGTTGCATTGAAGAAGGAGGGTGTTCCCTCAAACACCCTTCGACTGCAGCACCAGTAGCAGCAATTTCTTCGTCTCTGTTACGGTTGAAAAAGAGATTGAGAGAATCCGAGGAAACAGCAGAAGCAACAATTTCTTTGTCTCTATCACAGTCGAAAAAGCGACCAAGAGAATCCGAGGAAGCAGCAGCAATAGCAATTTCTTCAGAATCCGATGAATCAACTTCTGATGATGATTCTGAAGTCTCTAGTTGCAGTTCTGAATCAACTTCTGACGAGGATTTTGAAGTCTCGAGTTTCAGTTCGATTAAAAAATCTACGGTTACTACGGTGTCAACATCAATGGAAAATGAGAAGCGTGTGACTCGATCTGAACCAGAAGTTACTAATTCGGTGGGTAGTGGTAAAAGAGGAAAGGTTTTAAAAATGAGTACAATGGAGAAGAATTCAGACGATTGTGGTAGAAGAGGGAAAGTATTAAAGATGAGTATAATGGAGAAGAATTCAGAGGTTTACGGTAAGAGAGGGAAGGTATCAAAGAGGGAAGGTACAAGGGAAAGGTATTCACTTGTTTCTTGGTTGATAAAGAATCAAGTTTTGGTTTCTGGGACTACTGTGTTTTGTTGTGGTAGTAACAATGTTGTCAAAAGAGGGAGTTTATTTTATGATGGCATAGTTTGTGATTGTTGTCGTGTTAATTTCACTATCACTGGATTTGAGGCTCATGCTGGTTGTACAAGGCATAGACCATCCACTACTATCTTGTTGGAAGACGGAAGGTCATTGTTGGAATGTCAGAGGGAAGCTCAGAGTTCGCATGATGAAAAGGGGAATCATTGTGTGGCTGAAGCAGATTCTGAAGCACAAAATCATTGTGTTTCCGAAGCAAGTTCTGAAGTAGGGAATCACTGTGTTTCCAAAGCAAATAATGATGGTGTTTGTTCTATTTGTGGTTTGGGAGGTGATATAGTACTATGTGATCGATGTCCTTCTGCATTCCACCTCGTTTGCTTAGGTCTCGATCAAGTTCCGGATGGTGATTGGTTTTGTCCAACTTGTTGTTGCAAAATATGTTATCACCCAAAATGCAAACAACAATGTGCAGATGAAAATGATAACAATATTCTTGTTTGTGTTCAATGTGAGCAAAATTTTCACTTTGGATGTGTAAAGGCTACAAGATTTGGATCTAGTCACATGGAGAGCAATGTTAAAAACAGGAACTGGTTTTGCAGTGTAGTTTGCGGGAAGATGTTCTTCTGTCTGAAGAAGCTATTAGGGAAACCAATTAAGGTTGCAAATAAAATAAATTGGACACTGTTGAAGAATTTGAGTAGTGATGATGATGGCGGAGATTTTACTTCAAATGAGCTTAGTCAAAAGAAGAGAAAGTTGAATGTGGCTCTTGATGTATTGTATGAGAGTTTTAACCCAACTATTGATGCTTTATCTGGAAGAGAAATGATTAAAGATGTCGTTTTTAGTAGAGGCTCAAAACATAAACGCTTGAATTTCCATGGTTTCTATAATGTGATTTTAGAGAAAATGGGTAAAGTCATTTCGGTTGCAACTATAAGAATTTATGGTCAAAAGGTTGCCGAGATAGTTTTCGTGGCTACCAAGGAACAATATCGAAGACAAGGAATGTGTCGTTTGTTGATGCACGAGCTTGAGAAACAGTTGACACGCTTAGGAGTTGAAAGCTTGGTTTTGCATTCCTCCGAGGATGCTATAGACACGTGGACAAAAGCGTTTGGTTTTGCTAGGATAACCCGTGAAGACAAGTGTCAATTTGTTGATAAAACTTTCTTAGAATTTCAGAACACTATCATGTGCCTTAAATCCCTAAATAGACCTATATGGCCTTGTATTGCTTGA
Protein sequence

>MS.gene015078.t1

MDSCSPPPDFNIEPENCPEPNNAKNYISLRKPCESCIEEGGCSLKHPSTAAPVAAISSSLLRLKKRLRESEETAEATISLSLSQSKKRPRESEEAAAIAISSESDESTSDDDSEVSSCSSESTSDEDFEVSSFSSIKKSTVTTVSTSMENEKRVTRSEPEVTNSVGSGKRGKVLKMSTMEKNSDDCGRRGKVLKMSIMEKNSEVYGKRGKVSKREGTRERYSLVSWLIKNQVLVSGTTVFCCGSNNVVKRGSLFYDGIVCDCCRVNFTITGFEAHAGCTRHRPSTTILLEDGRSLLECQREAQSSHDEKGNHCVAEADSEAQNHCVSEASSEVGNHCVSKANNDGVCSICGLGGDIVLCDRCPSAFHLVCLGLDQVPDGDWFCPTCCCKICYHPKCKQQCADENDNNILVCVQCEQNFHFGCVKATRFGSSHMESNVKNRNWFCSVVCGKMFFCLKKLLGKPIKVANKINWTLLKNLSSDDDGGDFTSNELSQKKRKLNVALDVLYESFNPTIDALSGREMIKDVVFSRGSKHKRLNFHGFYNVILEKMGKVISVATIRIYGQKVAEIVFVATKEQYRRQGMCRLLMHELEKQLTRLGVESLVLHSSEDAIDTWTKAFGFARITREDKCQFVDKTFLEFQNTIMCLKSLNRPIWPCIA