AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015289

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015289.t1 MTR_5g014890 97.059 272 5 3 1 271 1 270 0.0 528
MS.gene015289.t1 MTR_4g097600 64.360 289 67 9 1 271 1 271 3.95e-98 288
MS.gene015289.t1 MTR_2g061030 70.548 146 40 2 94 237 169 313 2.14e-64 205
MS.gene015289.t1 MTR_1g054285 72.388 134 36 1 104 237 135 267 2.34e-63 201
MS.gene015289.t1 MTR_1g017090 57.868 197 73 6 73 266 83 272 3.32e-63 200
MS.gene015289.t1 MTR_5g013010 59.067 193 66 5 47 237 69 250 1.41e-62 199
MS.gene015289.t1 MTR_7g093430 70.455 132 39 0 106 237 142 273 1.54e-62 199
MS.gene015289.t1 MTR_4g100550 64.242 165 48 5 74 237 80 234 4.68e-61 194
MS.gene015289.t1 MTR_8g006705 61.765 170 55 3 74 235 91 258 6.87e-60 192
MS.gene015289.t1 MTR_3g103590 57.222 180 67 4 93 266 117 292 1.77e-50 168
MS.gene015289.t1 MTR_4g084750 58.955 134 49 1 123 250 64 197 8.04e-48 158
MS.gene015289.t1 MTR_2g038625 52.349 149 67 2 91 238 52 197 1.19e-40 140
MS.gene015289.t1 MTR_2g038580 52.349 149 67 2 91 238 52 197 1.19e-40 140
MS.gene015289.t1 MTR_4g126900 64.655 116 33 3 126 235 90 203 1.20e-37 132
MS.gene015289.t1 MTR_5g013010 53.846 130 48 4 47 175 69 187 9.52e-28 106
MS.gene015289.t1 MTR_5g039000 38.411 151 79 2 73 215 24 168 9.58e-20 86.7
MS.gene015289.t1 MTR_7g010020 45.833 96 52 0 115 210 71 166 1.32e-18 83.6
MS.gene015289.t1 MTR_8g468210 42.500 120 62 1 96 215 56 168 1.45e-18 83.6
MS.gene015289.t1 MTR_6g011610 45.794 107 51 1 111 210 83 189 1.96e-18 83.2
MS.gene015289.t1 MTR_6g007647 50.000 88 44 0 124 211 33 120 2.60e-18 82.4
MS.gene015289.t1 MTR_6g007647 48.913 92 47 0 124 215 67 158 3.97e-18 82.4
MS.gene015289.t1 MTR_6g011610 45.794 107 51 1 111 210 64 170 4.70e-18 81.6
MS.gene015289.t1 MTR_5g038280 40.559 143 72 4 78 211 43 181 1.12e-17 81.6
MS.gene015289.t1 MTR_7g103340 40.187 107 63 1 105 210 43 149 2.15e-16 76.6
MS.gene015289.t1 MTR_8g469430 46.809 94 43 1 124 210 89 182 2.53e-16 77.4
MS.gene015289.t1 MTR_1g061660 43.478 92 52 0 121 212 56 147 3.12e-16 76.3
MS.gene015289.t1 MTR_3g080100 47.368 95 43 1 124 211 20 114 3.90e-16 75.5
MS.gene015289.t1 MTR_3g080100 47.368 95 43 1 124 211 59 153 5.38e-16 75.9
MS.gene015289.t1 MTR_4g107650 45.833 96 52 0 116 211 87 182 6.73e-16 76.6
MS.gene015289.t1 MTR_3g086790 42.718 103 52 1 116 211 52 154 1.55e-15 75.5
MS.gene015289.t1 MTR_8g089895 46.392 97 45 1 122 211 52 148 2.06e-15 75.1
MS.gene015289.t1 MTR_8g026960 35.878 131 72 3 128 251 38 163 1.15e-12 66.2
MS.gene015289.t1 MTR_3g092150 43.617 94 52 1 127 220 41 133 6.68e-12 64.3
MS.gene015289.t1 MTR_5g019680 44.000 100 51 1 119 218 48 142 2.69e-11 62.0
MS.gene015289.t1 MTR_5g019650 44.086 93 52 0 119 211 48 140 4.23e-11 61.6
MS.gene015289.t1 MTR_5g029320 32.500 120 81 0 101 220 69 188 5.83e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015289.t1 AT4G37790 60.690 290 83 9 1 271 1 278 5.62e-93 276
MS.gene015289.t1 AT2G22800 57.093 289 91 8 1 271 1 274 1.10e-83 252
MS.gene015289.t1 AT5G06710 68.707 147 42 2 96 240 162 306 2.43e-65 207
MS.gene015289.t1 AT3G60390 59.091 176 58 3 74 235 99 274 8.19e-60 192
MS.gene015289.t1 AT4G16780 66.901 142 45 2 96 236 102 242 1.41e-59 191
MS.gene015289.t1 AT2G44910 54.822 197 76 4 49 236 84 276 2.69e-57 186
MS.gene015289.t1 AT5G47370 51.659 211 73 8 74 271 72 266 8.42e-56 181
MS.gene015289.t1 AT4G17460 55.747 174 66 5 74 237 77 249 3.08e-52 172
MS.gene015289.t1 AT2G01430 55.556 126 54 1 115 240 130 253 7.14e-41 142
MS.gene015289.t1 AT1G70920 51.852 135 54 2 116 246 27 154 2.22e-36 127
MS.gene015289.t1 AT1G70920 53.846 130 49 2 116 241 57 179 2.47e-36 129
MS.gene015289.t1 AT5G06710 70.732 82 20 2 96 175 162 241 4.29e-29 111
MS.gene015289.t1 AT2G01430 57.692 78 31 1 115 192 130 205 1.50e-21 90.1
MS.gene015289.t1 AT1G26960 41.593 113 66 0 116 228 63 175 1.86e-18 82.8
MS.gene015289.t1 AT1G69780 45.833 96 52 0 116 211 77 172 5.95e-18 82.0
MS.gene015289.t1 AT5G15150 42.500 120 62 2 98 213 92 208 9.71e-18 81.6
MS.gene015289.t1 AT3G01220 40.984 122 64 2 96 213 63 180 4.77e-17 79.3
MS.gene015289.t1 AT3G01470 47.312 93 42 1 124 209 68 160 8.58e-17 78.6
MS.gene015289.t1 AT5G65310 43.000 100 50 1 119 211 67 166 2.59e-15 74.7
MS.gene015289.t1 AT5G65310 43.000 100 50 1 119 211 49 148 2.87e-15 74.3
MS.gene015289.t1 AT4G40060 43.299 97 51 1 122 214 57 153 3.85e-15 74.3
MS.gene015289.t1 AT2G22430 44.792 96 49 1 122 213 60 155 2.13e-14 72.4
MS.gene015289.t1 AT5G03790 32.653 147 96 2 124 267 77 223 4.13e-14 70.5
MS.gene015289.t1 AT1G27045 46.875 96 44 1 124 212 68 163 4.88e-14 70.5
MS.gene015289.t1 AT1G27045 50.000 88 44 0 124 211 68 155 5.58e-14 69.7
MS.gene015289.t1 AT2G46680 42.424 99 53 1 121 215 29 127 7.41e-14 70.1
MS.gene015289.t1 AT2G46680 40.625 96 57 0 121 216 29 124 7.90e-14 70.1
MS.gene015289.t1 AT1G27045 57.576 66 28 0 124 189 25 90 9.34e-14 68.6
MS.gene015289.t1 AT1G27045 57.576 66 28 0 124 189 25 90 9.34e-14 68.6
MS.gene015289.t1 AT1G27045 57.576 66 28 0 124 189 17 82 1.19e-13 67.8
MS.gene015289.t1 AT2G18550 44.000 100 55 1 112 210 48 147 3.73e-13 67.4
MS.gene015289.t1 AT2G36610 42.857 98 48 2 123 212 70 167 4.64e-13 66.6
MS.gene015289.t1 AT5G03790 37.931 116 64 2 124 231 77 192 1.23e-11 62.8
MS.gene015289.t1 AT4G36740 43.678 87 49 0 124 210 55 141 1.23e-11 63.2
MS.gene015289.t1 AT4G36740 43.678 87 49 0 124 210 56 142 1.25e-11 63.2
MS.gene015289.t1 AT3G61890 42.353 85 49 0 127 211 33 117 2.68e-11 62.4

Find 65 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTACCTGGCTCTCCGATTC+TGG 0.197577 5.4:+8600201 None:intergenic
GATTCTCTTCACCTTGTTCT+AGG 0.242496 5.4:-8600811 MS.gene015289:CDS
AAACATTAACAGACGAAAAT+AGG 0.283618 5.4:-8599387 MS.gene015289:CDS
GGATTGTAGAAATGAGTATT+TGG 0.305343 5.4:+8599199 None:intergenic
AAGCAATAGTGATTGTTCTT+TGG 0.306534 5.4:+8600447 None:intergenic
TGGCTCGTTGGAGGTGGAAT+AGG 0.345900 5.4:+8600726 None:intergenic
CGAAAATAGGAGGCTCAAAA+AGG 0.353329 5.4:-8599374 MS.gene015289:CDS
TAGCGAGACAGTTGAATCTA+AGG 0.358273 5.4:-8600247 MS.gene015289:CDS
GTGGTGGTAGTTATTTCCTT+TGG 0.358754 5.4:+8600763 None:intergenic
ATAGGGATTCATGGTGGTGG+TGG 0.418056 5.4:+8600744 None:intergenic
AAGTTCCTCACCATAACCTT+TGG 0.418493 5.4:+8600645 None:intergenic
GGGATTCATGGTGGTGGTGG+TGG 0.429866 5.4:+8600747 None:intergenic
AAACAAGCTCCCAAAGGTTA+TGG 0.433046 5.4:-8600655 MS.gene015289:CDS
AAGAAGAAGTAGAAGAAGAG+AGG 0.435022 5.4:-8600534 MS.gene015289:CDS
GGCTCGTTGGAGGTGGAATA+GGG 0.435231 5.4:+8600727 None:intergenic
GGCCACGACAAGTTGAAGTA+TGG 0.447393 5.4:-8600226 MS.gene015289:CDS
GTTAATTCATCTTTCTCAAG+TGG 0.450640 5.4:-8600592 MS.gene015289:CDS
TCCTTAACGTTAGGACTCTC+TGG 0.452116 5.4:-8600703 MS.gene015289:CDS
ACCAGAGAGTCCTAACGTTA+AGG 0.454957 5.4:+8600702 None:intergenic
TTGAAGTATGGTTCCAGAAT+CGG 0.456231 5.4:-8600214 MS.gene015289:CDS
CGCGTCGCCGACGGTGGTGG+TGG 0.459139 5.4:-8599253 MS.gene015289:CDS
AGTTCCTCACCATAACCTTT+GGG 0.463230 5.4:+8600646 None:intergenic
CGCAAGAAGGGCAGATGGAG+AGG 0.463288 5.4:+8599287 None:intergenic
GGCCGCGTCGCCGACGGTGG+TGG 0.477523 5.4:-8599256 MS.gene015289:CDS
GGTTCCAGAATCGGAGAGCC+AGG 0.479376 5.4:-8600205 MS.gene015289:intron
TAAGGATGGCTCGTTGGAGG+TGG 0.501094 5.4:+8600720 None:intergenic
CAAATGCTAGAAAGAAACTT+AGG 0.503490 5.4:-8600474 MS.gene015289:CDS
GGAGGTGGAATAGGGATTCA+TGG 0.513429 5.4:+8600735 None:intergenic
CGTTAAGGATGGCTCGTTGG+AGG 0.516004 5.4:+8600717 None:intergenic
AGAAGAAGTAGAAGAAGAGA+GGG 0.516839 5.4:-8600533 MS.gene015289:CDS
CGGCCGAGTCTTTCGCAAGA+AGG 0.519322 5.4:+8599274 None:intergenic
CACCACCACCGTCGGCGACG+CGG 0.531823 5.4:+8599254 None:intergenic
AACGAGCCATCCTTAACGTT+AGG 0.533980 5.4:-8600712 MS.gene015289:CDS
GGCCGAGTCTTTCGCAAGAA+GGG 0.542654 5.4:+8599275 None:intergenic
GAGAGTGCTGCAAGTGAAGA+GGG 0.544213 5.4:-8600569 MS.gene015289:CDS
GAGAGGGTAGCTGCAGGCAT+AGG 0.545391 5.4:+8599304 None:intergenic
CAGATGGAGAGGGTAGCTGC+AGG 0.545980 5.4:+8599298 None:intergenic
TTAATTCATCTTTCTCAAGT+GGG 0.546761 5.4:-8600591 MS.gene015289:CDS
TTTGTTTAACATGCTGCGGA+AGG 0.548799 5.4:+8599166 None:intergenic
AGACTCGGCCGCGTCGCCGA+CGG 0.550522 5.4:-8599262 MS.gene015289:CDS
GATGAATTAACAACACTATG+AGG 0.550657 5.4:+8600604 None:intergenic
CTCGGCCGCGTCGCCGACGG+TGG 0.550736 5.4:-8599259 MS.gene015289:CDS
GAATACTTCTACTACTCCAA+AGG 0.557339 5.4:-8600779 MS.gene015289:CDS
GGAGAGTGCTGCAAGTGAAG+AGG 0.575107 5.4:-8600570 MS.gene015289:CDS
GAGCTTGTTTGTGATTTGTA+CGG 0.578033 5.4:+8600668 None:intergenic
GGAATAGGGATTCATGGTGG+TGG 0.578972 5.4:+8600741 None:intergenic
GAAATGAGTATTTGGAGCCA+TGG 0.579663 5.4:+8599207 None:intergenic
GCAAGAAGGGCAGATGGAGA+GGG 0.582206 5.4:+8599288 None:intergenic
ATTAGAACCACCACCACCGT+CGG 0.589643 5.4:+8599246 None:intergenic
TCTTTCGCAAGAAGGGCAGA+TGG 0.591761 5.4:+8599282 None:intergenic
GCTGCGGAAGGATTGTTGAA+AGG 0.595707 5.4:+8599178 None:intergenic
TGCCCTTCTTGCGAAAGACT+CGG 0.599355 5.4:-8599277 MS.gene015289:CDS
AGTATTTGGAGCCATGGTGA+AGG 0.599655 5.4:+8599213 None:intergenic
AACCATACTTCAACTTGTCG+TGG 0.610138 5.4:+8600224 None:intergenic
GAGAGTCCTAACGTTAAGGA+TGG 0.616116 5.4:+8600706 None:intergenic
TAACGTTAAGGATGGCTCGT+TGG 0.618624 5.4:+8600714 None:intergenic
CATTAACAGACGAAAATAGG+AGG 0.634319 5.4:-8599384 MS.gene015289:CDS
TAAAATCACTGCCTTCACCA+TGG 0.649046 5.4:-8599224 MS.gene015289:CDS
AAAGCTGAAACAAACAGAGG+TGG 0.651647 5.4:-8599437 MS.gene015289:CDS
AGCTCCCAAAGGTTATGGTG+AGG 0.658770 5.4:-8600650 MS.gene015289:CDS
AATCACAAACAAGCTCCCAA+AGG 0.673939 5.4:-8600661 MS.gene015289:CDS
GGTGGAATAGGGATTCATGG+TGG 0.677474 5.4:+8600738 None:intergenic
TCAAAGATAAACCTAGAACA+AGG 0.685669 5.4:+8600800 None:intergenic
GCAGGCATAGGCATGTACAA+AGG 0.695698 5.4:+8599316 None:intergenic
AACAAAGCTGAAACAAACAG+AGG 0.748355 5.4:-8599440 MS.gene015289:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATTATATTAATATTCTGT+TGG - chr5.4:8599704-8599723 MS.gene015289:intron 10.0%
!! CTAATAAAAAATAGTTAATT+TGG + chr5.4:8600350-8600369 None:intergenic 10.0%
!! TCTTAAAAATCTTATATTTA+AGG - chr5.4:8600034-8600053 MS.gene015289:intron 10.0%
!!! ATTTATTTTCTTATCTTATA+AGG - chr5.4:8600208-8600227 MS.gene015289:CDS 10.0%
!!! TTTATTTTCTTATCTTATAA+GGG - chr5.4:8600209-8600228 MS.gene015289:CDS 10.0%
!! AAAATTGCTCTTATAATTAT+TGG + chr5.4:8600287-8600306 None:intergenic 15.0%
!!! AAAAGTCTTATATTAAAGTA+AGG - chr5.4:8600402-8600421 MS.gene015289:intron 15.0%
!!! TGAGTTTTGTAAAAAAAAAA+AGG + chr5.4:8600116-8600135 None:intergenic 15.0%
!! CCTATATGTAAAATAAATAG+AGG + chr5.4:8600181-8600200 None:intergenic 20.0%
!! TAAAAGAGAGAGAAAAAATT+AGG - chr5.4:8600237-8600256 MS.gene015289:CDS 20.0%
!!! AATATAAGACTTTTTTTGAG+AGG + chr5.4:8600396-8600415 None:intergenic 20.0%
!!! CCTCTATTTATTTTACATAT+AGG - chr5.4:8600178-8600197 MS.gene015289:intron 20.0%
!!! GATATTTTATGAATATCATG+TGG - chr5.4:8599991-8600010 MS.gene015289:intron 20.0%
!!! TAATTTTCATGACTCTATAA+TGG + chr5.4:8599865-8599884 None:intergenic 20.0%
! AAACATTAACAGACGAAAAT+AGG - chr5.4:8600616-8600635 MS.gene015289:CDS 25.0%
! GAGATGAGAAAAATAATTAC+AGG + chr5.4:8599615-8599634 None:intergenic 25.0%
! GTCTTATATTAAAGTAAGGA+GGG - chr5.4:8600406-8600425 MS.gene015289:intron 25.0%
! TTAATTCATCTTTCTCAAGT+GGG - chr5.4:8599412-8599431 MS.gene015289:CDS 25.0%
!! AGTCTTATATTAAAGTAAGG+AGG - chr5.4:8600405-8600424 MS.gene015289:intron 25.0%
AAGCAATAGTGATTGTTCTT+TGG + chr5.4:8599559-8599578 None:intergenic 30.0%
AATAAATGTTGGTGCATGTT+TGG - chr5.4:8600500-8600519 MS.gene015289:CDS 30.0%
CAAATGCTAGAAAGAAACTT+AGG - chr5.4:8599529-8599548 MS.gene015289:intron 30.0%
CAGACTAAGCTAATAAATGT+TGG - chr5.4:8600489-8600508 MS.gene015289:CDS 30.0%
GATGAATTAACAACACTATG+AGG + chr5.4:8599402-8599421 None:intergenic 30.0%
GGATTGTAGAAATGAGTATT+TGG + chr5.4:8600807-8600826 None:intergenic 30.0%
GTTAATTCATCTTTCTCAAG+TGG - chr5.4:8599411-8599430 MS.gene015289:CDS 30.0%
TCAAAGATAAACCTAGAACA+AGG + chr5.4:8599206-8599225 None:intergenic 30.0%
AACAAAGCTGAAACAAACAG+AGG - chr5.4:8600563-8600582 MS.gene015289:CDS 35.0%
AACCACAAATCCAAAAGCAA+AGG + chr5.4:8600452-8600471 None:intergenic 35.0%
AAGAAGAAGTAGAAGAAGAG+AGG - chr5.4:8599469-8599488 MS.gene015289:intron 35.0%
ACAAATCCAAAAGCAAAGGA+AGG + chr5.4:8600448-8600467 None:intergenic 35.0%
AGAAGAAGTAGAAGAAGAGA+GGG - chr5.4:8599470-8599489 MS.gene015289:intron 35.0%
CATTAACAGACGAAAATAGG+AGG - chr5.4:8600619-8600638 MS.gene015289:CDS 35.0%
CTGTCTAGCTACTAAATTTG+TGG + chr5.4:8599940-8599959 None:intergenic 35.0%
GAATACTTCTACTACTCCAA+AGG - chr5.4:8599224-8599243 MS.gene015289:CDS 35.0%
GGATTTGTGGTTGTTAAGTA+TGG - chr5.4:8600460-8600479 MS.gene015289:CDS 35.0%
TTGAAGTATGGTTCCAGAAT+CGG - chr5.4:8599789-8599808 MS.gene015289:intron 35.0%
! AAAAAGCCTTCCTTTGCTTT+TGG - chr5.4:8600439-8600458 MS.gene015289:CDS 35.0%
! GACTCTATAATGGTGATGTA+CGG + chr5.4:8599855-8599874 None:intergenic 35.0%
! GAGCTTGTTTGTGATTTGTA+CGG + chr5.4:8599338-8599357 None:intergenic 35.0%
!! TTCCTTTGCTTTTGGATTTG+TGG - chr5.4:8600447-8600466 MS.gene015289:CDS 35.0%
AAACAAGCTCCCAAAGGTTA+TGG - chr5.4:8599348-8599367 MS.gene015289:CDS 40.0%
AAAGCTGAAACAAACAGAGG+TGG - chr5.4:8600566-8600585 MS.gene015289:CDS 40.0%
AACCATACTTCAACTTGTCG+TGG + chr5.4:8599782-8599801 None:intergenic 40.0%
AAGTTCCTCACCATAACCTT+TGG + chr5.4:8599361-8599380 None:intergenic 40.0%
AATCACAAACAAGCTCCCAA+AGG - chr5.4:8599342-8599361 MS.gene015289:CDS 40.0%
AGTTCCTCACCATAACCTTT+GGG + chr5.4:8599360-8599379 None:intergenic 40.0%
CGAAAATAGGAGGCTCAAAA+AGG - chr5.4:8600629-8600648 MS.gene015289:CDS 40.0%
GATTCTCTTCACCTTGTTCT+AGG - chr5.4:8599192-8599211 MS.gene015289:CDS 40.0%
TAAAATCACTGCCTTCACCA+TGG - chr5.4:8600779-8600798 MS.gene015289:CDS 40.0%
TAGCGAGACAGTTGAATCTA+AGG - chr5.4:8599756-8599775 MS.gene015289:intron 40.0%
! GAAATGAGTATTTGGAGCCA+TGG + chr5.4:8600799-8600818 None:intergenic 40.0%
! GTGGTGGTAGTTATTTCCTT+TGG + chr5.4:8599243-8599262 None:intergenic 40.0%
AACGAGCCATCCTTAACGTT+AGG - chr5.4:8599291-8599310 MS.gene015289:CDS 45.0%
ACCAGAGAGTCCTAACGTTA+AGG + chr5.4:8599304-8599323 None:intergenic 45.0%
GAGAGTCCTAACGTTAAGGA+TGG + chr5.4:8599300-8599319 None:intergenic 45.0%
TAACGTTAAGGATGGCTCGT+TGG + chr5.4:8599292-8599311 None:intergenic 45.0%
TCCTTAACGTTAGGACTCTC+TGG - chr5.4:8599300-8599319 MS.gene015289:CDS 45.0%
! AGTATTTGGAGCCATGGTGA+AGG + chr5.4:8600793-8600812 None:intergenic 45.0%
!!! AACTTATAATAAATAAATTT+TGG - chr5.4:8600526-8600545 MS.gene015289:CDS 5.0%
AGCTCCCAAAGGTTATGGTG+AGG - chr5.4:8599353-8599372 MS.gene015289:CDS 50.0%
AGTTTATGTCCGTCCGTACC+TGG + chr5.4:8599819-8599838 None:intergenic 50.0%
ATTAGAACCACCACCACCGT+CGG + chr5.4:8600760-8600779 None:intergenic 50.0%
GCAGGCATAGGCATGTACAA+AGG + chr5.4:8600690-8600709 None:intergenic 50.0%
GCTGCGGAAGGATTGTTGAA+AGG + chr5.4:8600828-8600847 None:intergenic 50.0%
GGAATAGGGATTCATGGTGG+TGG + chr5.4:8599265-8599284 None:intergenic 50.0%
GGAGGTGGAATAGGGATTCA+TGG + chr5.4:8599271-8599290 None:intergenic 50.0%
GGTGGAATAGGGATTCATGG+TGG + chr5.4:8599268-8599287 None:intergenic 50.0%
TCTTTCGCAAGAAGGGCAGA+TGG + chr5.4:8600724-8600743 None:intergenic 50.0%
TGCCCTTCTTGCGAAAGACT+CGG - chr5.4:8600726-8600745 MS.gene015289:CDS 50.0%
! GGCCACGACAAGTTGAAGTA+TGG - chr5.4:8599777-8599796 MS.gene015289:intron 50.0%
!! ATAGGGATTCATGGTGGTGG+TGG + chr5.4:8599262-8599281 None:intergenic 50.0%
!! GAGAGTGCTGCAAGTGAAGA+GGG - chr5.4:8599434-8599453 MS.gene015289:CDS 50.0%
CAGAATCGGAGAGCCAGGTA+CGG - chr5.4:8599803-8599822 MS.gene015289:intron 55.0%
CGTTAAGGATGGCTCGTTGG+AGG + chr5.4:8599289-8599308 None:intergenic 55.0%
GCAAGAAGGGCAGATGGAGA+GGG + chr5.4:8600718-8600737 None:intergenic 55.0%
GGCCGAGTCTTTCGCAAGAA+GGG + chr5.4:8600731-8600750 None:intergenic 55.0%
GGCTCGTTGGAGGTGGAATA+GGG + chr5.4:8599279-8599298 None:intergenic 55.0%
TAAGGATGGCTCGTTGGAGG+TGG + chr5.4:8599286-8599305 None:intergenic 55.0%
TGGCTCGTTGGAGGTGGAAT+AGG + chr5.4:8599280-8599299 None:intergenic 55.0%
!! GGAGAGTGCTGCAAGTGAAG+AGG - chr5.4:8599433-8599452 MS.gene015289:CDS 55.0%
ATCGGAGAGCCAGGTACGGA+CGG - chr5.4:8599807-8599826 MS.gene015289:intron 60.0%
CAGATGGAGAGGGTAGCTGC+AGG + chr5.4:8600708-8600727 None:intergenic 60.0%
CGCAAGAAGGGCAGATGGAG+AGG + chr5.4:8600719-8600738 None:intergenic 60.0%
CGGCCGAGTCTTTCGCAAGA+AGG + chr5.4:8600732-8600751 None:intergenic 60.0%
GAGAGGGTAGCTGCAGGCAT+AGG + chr5.4:8600702-8600721 None:intergenic 60.0%
GGTTCCAGAATCGGAGAGCC+AGG - chr5.4:8599798-8599817 MS.gene015289:intron 60.0%
! CGTACCTGGCTCTCCGATTC+TGG + chr5.4:8599805-8599824 None:intergenic 60.0%
!! GGGATTCATGGTGGTGGTGG+TGG + chr5.4:8599259-8599278 None:intergenic 60.0%
AGACTCGGCCGCGTCGCCGA+CGG - chr5.4:8600741-8600760 MS.gene015289:CDS 75.0%
CACCACCACCGTCGGCGACG+CGG + chr5.4:8600752-8600771 None:intergenic 75.0%
!! CGCGTCGCCGACGGTGGTGG+TGG - chr5.4:8600750-8600769 MS.gene015289:CDS 80.0%
! CTCGGCCGCGTCGCCGACGG+TGG - chr5.4:8600744-8600763 MS.gene015289:CDS 85.0%
! GGCCGCGTCGCCGACGGTGG+TGG - chr5.4:8600747-8600766 MS.gene015289:CDS 85.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 8599171 8600854 8599171 ID=MS.gene015289
chr5.4 mRNA 8599171 8600854 8599171 ID=MS.gene015289.t1;Parent=MS.gene015289
chr5.4 exon 8600411 8600854 8600411 ID=MS.gene015289.t1.exon1;Parent=MS.gene015289.t1
chr5.4 CDS 8600411 8600854 8600411 ID=cds.MS.gene015289.t1;Parent=MS.gene015289.t1
chr5.4 exon 8600206 8600285 8600206 ID=MS.gene015289.t1.exon2;Parent=MS.gene015289.t1
chr5.4 CDS 8600206 8600285 8600206 ID=cds.MS.gene015289.t1;Parent=MS.gene015289.t1
chr5.4 exon 8599171 8599462 8599171 ID=MS.gene015289.t1.exon3;Parent=MS.gene015289.t1
chr5.4 CDS 8599171 8599462 8599171 ID=cds.MS.gene015289.t1;Parent=MS.gene015289.t1
Gene Sequence

>MS.gene015289

ATGGGTCTTAATAATGATCAAGATTCTCTTCACCTTGTTCTAGGTTTATCTTTGAATACTTCTACTACTCCAAAGGAAATAACTACCACCACCACCACCATGAATCCCTATTCCACCTCCAACGAGCCATCCTTAACGTTAGGACTCTCTGGTGAGAGTTATAACCGTACAAATCACAAACAAGCTCCCAAAGGTTATGGTGAGGAACTTTGTAGACAAACTTCATCACCTCATAGTGTTGTTAATTCATCTTTCTCAAGTGGGAGAGTGCTGCAAGTGAAGAGGGAGAGAGATGAAGAAGAAGAAGTAGAAGAAGAGAGGGTTTCTTCAAGAGTTAGTGATGAAGATGAAGATGCTACAAATGCTAGAAAGAAACTTAGGCTTACCAAAGAACAATCACTATTGCTTGAAGAAAGCTTCAAACTACATAGCACTCTCAATCCTGTAATTATTTTTCTCATCTCTTCTAAATTCTAATTAGTTAGTGAAAATTAAATTAACTAATTAAATAATTAGAGTAATGATTAATAATAATATTATATTAATATTCTGTTGGATTTGATTTTCAGAAACAAAAACAAGCTTTAGCGAGACAGTTGAATCTAAGGCCACGACAAGTTGAAGTATGGTTCCAGAATCGGAGAGCCAGGTACGGACGGACATAAACTTTTTCATTATATACCGTACATCACCATTATAGAGTCATGAAAATTAATATTGAGATATATTTAAATAAATTGATACAAAAAAATTATGTAATTATCTACCACAAATTTAGTAGCTAGACAGAGTTTCTGCATTTTTTTTGTTGCTATTTCTAGATATTTTATGAATATCATGTGGTATATTGTATGTATAAATCTTCTTAAAAATCTTATATTTAAGGATAATATTTTTGATAAAAAAATTAATAGTAATTTTTTTTAACATCTATTAATTTGTCCTTTTTTTTTTTACAAAACTCATTTTGCATTATTTATTATATTAATTATTTTTTTACTAAATCACCTCTATTTATTTTACATATAGGAAATATAATTTATTTTCTTATCTTATAAGGGTTATATAAAAGAGAGAGAAAAAATTAGGATAATAATATATACAACTATGTTGCCAATAATTATAAGAGCAATTTTAAAAATCGATACAGATTATTCACAAATTTATTATTATTAACCAAATTAACTATTTTTTATTAGTTAAATAATTTTTTTTATTAAATCCTCTCAAAAAAAGTCTTATATTAAAGTAAGGAGGGAATATAATTTAAAAAGCCTTCCTTTGCTTTTGGATTTGTGGTTGTTAAGTATGGATTTGACAGACTAAGCTAATAAATGTTGGTGCATGTTTGGTGTAACTTATAATAAATAAATTTTGGTTTGAATTTTACAGAACAAAGCTGAAACAAACAGAGGTGGATTGCGAGTTCTTGAAGAAATGTTGTGAAACATTAACAGACGAAAATAGGAGGCTCAAAAAGGAGTTGCAAGAGTTGAAATCATTGAAAGTAGCACAACCTTTGTACATGCCTATGCCTGCAGCTACCCTCTCCATCTGCCCTTCTTGCGAAAGACTCGGCCGCGTCGCCGACGGTGGTGGTGGTTCTAATAAAATCACTGCCTTCACCATGGCTCCAAATACTCATTTCTACAATCCTTTCAACAATCCTTCCGCAGCATGTTAA
Protein sequence

>MS.gene015289.t1

MGLNNDQDSLHLVLGLSLNTSTTPKEITTTTTTMNPYSTSNEPSLTLGLSGESYNRTNHKQAPKGYGEELCRQTSSPHSVVNSSFSSGRVLQVKRERDEEEEVEEERVSSRVSDEDEDATNARKKLRLTKEQSLLLEESFKLHSTLNPKQKQALARQLNLRPRQVEVWFQNRRARTKLKQTEVDCEFLKKCCETLTDENRRLKKELQELKSLKVAQPLYMPMPAATLSICPSCERLGRVADGGGGSNKITAFTMAPNTHFYNPFNNPSAAC