AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015300

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015300.t1 MTR_5g014640 94.817 328 15 1 1 328 1 326 0.0 615
MS.gene015300.t1 MTR_4g097940 47.458 354 126 15 1 324 1 324 4.37e-83 254
MS.gene015300.t1 MTR_4g097920 44.633 354 136 15 1 324 1 324 3.31e-77 239
MS.gene015300.t1 MTR_4g097920 44.248 339 130 14 15 324 1 309 4.05e-72 226
MS.gene015300.t1 MTR_2g010450 35.328 351 191 11 2 323 5 348 2.78e-53 179
MS.gene015300.t1 MTR_4g092700 34.161 322 184 10 15 324 24 329 2.22e-50 171
MS.gene015300.t1 MTR_5g014600 40.336 238 121 6 104 325 102 334 1.94e-47 163
MS.gene015300.t1 MTR_5g014520 38.403 263 142 4 63 323 83 327 2.77e-46 160
MS.gene015300.t1 MTR_4g009110 38.060 268 135 7 68 325 103 349 1.41e-45 158
MS.gene015300.t1 MTR_2g104550 36.434 258 138 6 75 325 73 311 9.57e-44 152
MS.gene015300.t1 MTR_4g009540 40.265 226 116 4 116 325 108 330 1.14e-43 153
MS.gene015300.t1 MTR_0250s0040 42.614 176 100 1 151 325 85 260 8.73e-43 149
MS.gene015300.t1 MTR_4g067010 41.436 181 100 2 147 325 140 316 7.62e-42 148
MS.gene015300.t1 MTR_2g104560 41.081 185 100 5 145 325 45 224 1.40e-41 144
MS.gene015300.t1 MTR_8g009020 39.683 189 102 3 147 325 136 322 9.38e-41 145
MS.gene015300.t1 MTR_0011s0210 43.353 173 95 2 152 322 181 352 7.25e-40 144
MS.gene015300.t1 MTR_0011s0260 43.023 172 97 1 152 322 181 352 1.02e-39 143
MS.gene015300.t1 MTR_5g014560 36.948 249 142 7 82 325 65 303 2.06e-39 141
MS.gene015300.t1 MTR_4g066460 41.262 206 104 4 126 325 130 324 5.38e-39 140
MS.gene015300.t1 MTR_0246s0020 40.704 199 102 6 138 325 181 374 7.91e-39 141
MS.gene015300.t1 MTR_4g009540 39.806 206 105 4 116 305 108 310 1.04e-38 139
MS.gene015300.t1 MTR_6g047570 40.805 174 102 1 149 321 154 327 3.80e-38 139
MS.gene015300.t1 MTR_7g080780 40.223 179 99 1 147 325 158 328 1.03e-37 137
MS.gene015300.t1 MTR_7g080780 40.223 179 99 1 147 325 145 315 1.04e-37 137
MS.gene015300.t1 MTR_2g104490 34.661 251 138 6 87 324 69 306 3.28e-37 135
MS.gene015300.t1 MTR_0246s0020 43.017 179 86 6 138 305 181 354 2.27e-36 134
MS.gene015300.t1 MTR_4g066380 38.308 201 116 4 126 325 128 321 6.39e-36 132
MS.gene015300.t1 MTR_8g069740 34.978 223 124 6 88 304 58 265 4.25e-34 126
MS.gene015300.t1 MTR_2g104490 34.599 237 129 6 87 310 69 292 5.23e-34 126
MS.gene015300.t1 MTR_2g104650 41.398 186 102 3 147 327 87 270 7.42e-34 125
MS.gene015300.t1 MTR_0246s0050 35.263 190 114 3 138 325 134 316 1.57e-33 126
MS.gene015300.t1 MTR_4g098035 35.263 190 114 3 138 325 155 337 2.72e-33 125
MS.gene015300.t1 MTR_4g097950 36.538 208 114 3 133 325 45 249 4.52e-33 123
MS.gene015300.t1 MTR_2g104590 34.184 196 102 4 137 327 52 225 5.98e-32 119
MS.gene015300.t1 MTR_8g009010 36.932 176 75 2 147 321 127 267 7.20e-32 121
MS.gene015300.t1 MTR_2g104500 38.286 175 98 3 154 325 104 271 4.18e-31 118
MS.gene015300.t1 MTR_2g104530 34.545 220 121 4 111 325 72 273 7.52e-31 118
MS.gene015300.t1 MTR_4g009420 36.424 151 79 1 177 327 65 198 1.39e-21 90.9
MS.gene015300.t1 MTR_6g047550 63.636 66 24 0 149 214 168 233 3.11e-21 91.7
MS.gene015300.t1 MTR_7g451650 34.437 151 90 1 177 327 57 198 2.91e-20 87.4
MS.gene015300.t1 MTR_4g009420 38.462 130 63 1 177 306 65 177 3.34e-20 87.4
MS.gene015300.t1 MTR_8g067280 36.196 163 70 5 147 284 451 604 3.36e-20 91.7
MS.gene015300.t1 MTR_5g014550 36.220 127 78 2 184 308 54 179 1.33e-19 85.5
MS.gene015300.t1 MTR_4g009510 31.707 164 101 3 165 327 53 206 1.41e-19 85.9
MS.gene015300.t1 MTR_7g451650 36.154 130 74 1 177 306 57 177 2.73e-19 84.7
MS.gene015300.t1 MTR_1g072320 32.787 183 105 6 155 324 471 648 3.81e-18 85.5
MS.gene015300.t1 MTR_5g030430 34.810 158 87 2 147 288 482 639 1.16e-17 84.3
MS.gene015300.t1 MTR_7g050350 32.394 142 87 1 183 324 1 133 1.26e-17 78.6
MS.gene015300.t1 MTR_7g451650 32.394 142 87 1 183 324 1 133 1.26e-17 78.6
MS.gene015300.t1 MTR_7g117670 37.705 122 51 3 155 266 403 509 1.34e-17 84.0
MS.gene015300.t1 MTR_7g083900 30.481 187 110 4 154 326 286 466 1.23e-16 80.9
MS.gene015300.t1 MTR_8g027495 37.391 115 53 2 155 263 459 560 1.50e-16 80.9
MS.gene015300.t1 MTR_8g024790 30.636 173 115 2 155 322 292 464 2.31e-16 80.1
MS.gene015300.t1 MTR_7g083900 29.947 187 112 4 154 326 286 467 6.53e-16 78.6
MS.gene015300.t1 MTR_8g024770 36.029 136 71 4 155 278 280 411 2.62e-15 76.6
MS.gene015300.t1 MTR_3g030700 28.638 213 128 7 130 324 166 372 4.94e-15 75.5
MS.gene015300.t1 MTR_8g064700 25.146 171 122 2 138 302 228 398 1.05e-14 74.7
MS.gene015300.t1 MTR_1g112680 32.836 134 81 3 86 218 256 381 4.17e-13 70.1
MS.gene015300.t1 MTR_3g498695 28.409 176 106 5 154 316 298 466 4.78e-13 70.1
MS.gene015300.t1 MTR_7g096350 34.146 123 54 3 145 263 342 441 6.01e-13 69.7
MS.gene015300.t1 MTR_3g498695 29.560 159 97 4 154 299 298 454 1.09e-12 68.9
MS.gene015300.t1 MTR_4g057270 31.206 141 82 4 156 287 151 285 1.87e-12 67.4
MS.gene015300.t1 MTR_5g030770 26.596 282 170 9 68 325 47 315 2.10e-12 67.4
MS.gene015300.t1 MTR_8g098275 28.313 166 83 4 155 287 402 564 2.86e-12 67.8
MS.gene015300.t1 MTR_8g098275 28.313 166 83 4 155 287 309 471 2.98e-12 67.8
MS.gene015300.t1 MTR_8g098275 28.313 166 83 4 155 287 245 407 3.15e-12 67.4
MS.gene015300.t1 MTR_3g111290 29.379 177 106 4 150 325 169 327 9.25e-12 65.5
MS.gene015300.t1 MTR_2g081040 28.342 187 109 5 156 324 179 358 9.45e-12 65.5
MS.gene015300.t1 MTR_2g084280 30.714 140 84 4 156 289 135 267 1.96e-11 64.3
MS.gene015300.t1 MTR_3g104100 26.738 187 112 5 156 324 197 376 4.07e-11 63.9
MS.gene015300.t1 MTR_2g038040 24.352 193 130 5 132 309 29 220 4.15e-11 62.8
MS.gene015300.t1 MTR_5g030770 26.596 282 164 9 68 325 47 309 8.69e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015300.t1 AT4G37850 35.988 339 186 13 2 324 1 324 6.05e-49 167
MS.gene015300.t1 AT4G37850 35.988 339 186 13 2 324 1 324 6.05e-49 167
MS.gene015300.t1 AT2G22760 44.324 185 98 2 146 325 111 295 4.52e-47 161
MS.gene015300.t1 AT2G22760 45.732 164 85 1 146 305 111 274 1.14e-43 152
MS.gene015300.t1 AT2G22750 33.819 343 153 14 2 306 5 311 6.31e-42 148
MS.gene015300.t1 AT2G22750 33.819 343 153 14 2 306 1 307 7.98e-42 148
MS.gene015300.t1 AT2G22750 33.819 343 153 14 2 306 5 311 9.30e-42 148
MS.gene015300.t1 AT2G22750 45.763 177 69 4 146 306 118 283 4.34e-39 140
MS.gene015300.t1 AT2G22750 45.763 177 69 4 146 306 118 283 5.94e-39 140
MS.gene015300.t1 AT2G22770 40.284 211 108 3 132 324 108 318 2.27e-38 139
MS.gene015300.t1 AT4G17880 29.675 246 127 8 73 289 324 552 2.05e-20 92.4
MS.gene015300.t1 AT5G46760 34.356 163 80 4 145 289 402 555 3.27e-19 89.0
MS.gene015300.t1 AT1G32640 35.099 151 92 3 145 289 439 589 5.66e-17 82.0
MS.gene015300.t1 AT1G01260 33.529 170 82 5 150 313 429 573 3.52e-16 79.7
MS.gene015300.t1 AT1G01260 33.529 170 82 5 150 313 429 573 3.52e-16 79.7
MS.gene015300.t1 AT1G01260 33.529 170 82 5 150 313 429 573 3.52e-16 79.7
MS.gene015300.t1 AT2G46510 26.667 180 111 3 145 320 382 544 3.59e-16 79.7
MS.gene015300.t1 AT5G57150 26.344 186 117 3 132 302 32 212 1.59e-15 75.1
MS.gene015300.t1 AT5G57150 26.344 186 117 3 132 302 33 213 1.99e-15 74.7
MS.gene015300.t1 AT5G57150 26.344 186 117 3 132 302 33 213 2.20e-15 74.3
MS.gene015300.t1 AT5G57150 26.344 186 117 3 132 302 33 213 2.91e-15 75.1
MS.gene015300.t1 AT1G12860 29.444 180 115 4 154 326 267 441 3.75e-15 76.3
MS.gene015300.t1 AT5G57150 26.344 186 117 3 132 302 32 212 3.78e-15 74.3
MS.gene015300.t1 AT1G12860 31.373 153 98 3 154 299 267 419 8.01e-15 75.1
MS.gene015300.t1 AT4G09820 34.884 129 63 2 155 282 364 472 1.59e-14 74.7
MS.gene015300.t1 AT2G16910 28.070 171 97 5 155 299 315 485 8.33e-14 72.4
MS.gene015300.t1 AT1G63650 29.082 196 101 7 155 324 406 589 1.72e-13 71.6
MS.gene015300.t1 AT1G63650 29.082 196 101 7 155 324 406 589 1.72e-13 71.6
MS.gene015300.t1 AT1G63650 29.082 196 101 7 155 324 406 589 1.72e-13 71.6
MS.gene015300.t1 AT4G29930 30.137 146 89 3 154 296 54 189 1.95e-13 69.3
MS.gene015300.t1 AT4G29930 30.822 146 89 3 155 296 55 192 2.48e-13 68.9
MS.gene015300.t1 AT4G29930 30.345 145 88 3 155 296 55 189 2.94e-13 68.6
MS.gene015300.t1 AT3G26744 29.143 175 108 4 154 317 307 476 1.52e-12 68.6
MS.gene015300.t1 AT3G26744 29.143 175 108 4 154 317 307 476 1.52e-12 68.6
MS.gene015300.t1 AT3G26744 29.143 175 108 4 154 317 307 476 1.52e-12 68.6
MS.gene015300.t1 AT5G65640 28.889 180 112 5 150 325 174 341 3.24e-12 67.0
MS.gene015300.t1 AT3G26744 30.573 157 98 3 154 299 307 463 4.44e-12 67.0
MS.gene015300.t1 AT4G00870 29.927 137 92 1 155 287 250 386 5.30e-12 66.6
MS.gene015300.t1 AT4G16430 45.333 75 37 1 145 215 307 381 5.49e-12 66.6
MS.gene015300.t1 AT3G61950 30.597 134 88 3 155 284 129 261 1.03e-11 65.1
MS.gene015300.t1 AT3G61950 30.597 134 88 3 155 284 180 312 1.52e-11 65.1
MS.gene015300.t1 AT4G29930 26.667 165 99 3 154 296 54 218 1.55e-11 64.3
MS.gene015300.t1 AT4G29930 26.667 165 99 3 154 296 54 218 1.55e-11 64.3
MS.gene015300.t1 AT3G61950 30.827 133 89 2 155 284 129 261 1.85e-11 64.3
MS.gene015300.t1 AT4G29930 26.829 164 98 3 155 296 55 218 1.93e-11 63.9
MS.gene015300.t1 AT5G41315 28.788 198 104 8 155 324 442 630 2.08e-11 65.5
MS.gene015300.t1 AT5G41315 28.788 198 104 8 155 324 447 635 2.15e-11 65.1
MS.gene015300.t1 AT5G41315 28.788 198 104 8 155 324 450 638 2.17e-11 65.1
MS.gene015300.t1 AT3G61950 30.597 134 88 3 155 284 180 312 2.28e-11 64.7
MS.gene015300.t1 AT2G46810 28.729 181 118 4 150 322 118 295 9.86e-11 62.4

Find 62 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTTCAACTGGTTAATTT+TGG 0.110602 5.4:-8453916 None:intergenic
GATACTCTTACTTCGATTTC+AGG 0.236620 5.4:-8453102 None:intergenic
GTTTCAACTGGGAAACAATT+TGG 0.250112 5.4:-8451887 None:intergenic
TACTTTGGACAGTGAGATTA+AGG 0.260165 5.4:-8453203 None:intergenic
TTCAGGAAGTGGTTGGTTAT+TGG 0.276191 5.4:-8453085 None:intergenic
GCTCTTTCAGCCATTGTTCC+TGG 0.288584 5.4:+8452256 MS.gene015300:CDS
CCAAAGTTGTCGAATAATAT+TGG 0.304765 5.4:-8451836 None:intergenic
TCGAGCTTGAGCAGTGTTCC+TGG 0.329696 5.4:-8452179 None:intergenic
CATTGTTCCTGGCCTCAAAA+AGG 0.384959 5.4:+8452267 MS.gene015300:CDS
TCATCAAGATGAGTTTCAAC+TGG 0.389714 5.4:-8451899 None:intergenic
CTTCCAGCTTTGGTTTCTCA+AGG 0.393979 5.4:+8452103 MS.gene015300:CDS
AATGAGTTGAGGTGATGAAT+AGG 0.399012 5.4:-8451969 None:intergenic
TAAAATTGCTTAGAAGCAAT+TGG 0.399867 5.4:-8452016 None:intergenic
GTTGTTTCCAAAGGGCAAAA+AGG 0.400410 5.4:-8453229 None:intergenic
CCAATATTATTCGACAACTT+TGG 0.407380 5.4:+8451836 MS.gene015300:CDS
AGTAGTATGCTTACAAGTTC+AGG 0.414356 5.4:-8451555 None:intergenic
AGAAGCAATTGGTGTTGAAC+TGG 0.425456 5.4:-8452005 None:intergenic
TGAGGTGATGAATAGGAAGA+AGG 0.434612 5.4:-8451962 None:intergenic
ATGGACAAAGCTTCTGTGTT+GGG 0.439391 5.4:+8452913 MS.gene015300:CDS
TGTGACAGCAAAGGATCTCC+TGG 0.446189 5.4:+8453868 MS.gene015300:CDS
CAAGAGAATCCATCGGGTAT+TGG 0.448559 5.4:-8451808 None:intergenic
TGAAAGAGCGATGAAACTTC+GGG 0.456547 5.4:-8452242 None:intergenic
GAGGTGATGAATAGGAAGAA+GGG 0.466954 5.4:-8451961 None:intergenic
GGACGTGCCGCTACGGTGCT+TGG 0.467945 5.4:+8453168 MS.gene015300:CDS
ATCAACAATGTTGTTTCCAA+AGG 0.469768 5.4:-8453238 None:intergenic
TCAACAATGTTGTTTCCAAA+GGG 0.475298 5.4:-8453237 None:intergenic
CTGAAAGAGCGATGAAACTT+CGG 0.488970 5.4:-8452243 None:intergenic
AAGCCTGTCGTATGCTTCCC+AGG 0.497417 5.4:-8453886 None:intergenic
CTTCGATTTCAGGAAGTGGT+TGG 0.508259 5.4:-8453092 None:intergenic
TAAAACCTAAAGTCGAGAAA+AGG 0.508413 5.4:+8452059 MS.gene015300:CDS
TGTTCCTGGTCTGATTCACT+TGG 0.510038 5.4:-8452165 None:intergenic
CTTACTTCGATTTCAGGAAG+TGG 0.529255 5.4:-8453096 None:intergenic
TTTATTTACCTTAGCAACAA+TGG 0.529541 5.4:-8453265 None:intergenic
GAACCTTGAGAAACCAAAGC+TGG 0.540922 5.4:-8452106 None:intergenic
TGATGTAACCATTGTTGCTA+AGG 0.545162 5.4:+8453257 MS.gene015300:CDS
ACAAGTTCAGGCACATAGGT+GGG 0.548457 5.4:-8451543 None:intergenic
ACAAGCACGTTTGCAGACAC+TGG 0.553923 5.4:+8452963 MS.gene015300:CDS
ACACAGTGGACGTGCCGCTA+CGG 0.562208 5.4:+8453161 MS.gene015300:CDS
AATTCTCTTGCAGATGAACA+AGG 0.572870 5.4:+8453838 MS.gene015300:intron
CAAGTTCAGGCACATAGGTG+GGG 0.574321 5.4:-8451542 None:intergenic
CTTCTTTAACCAATACCCGA+TGG 0.574424 5.4:+8451799 MS.gene015300:CDS
AACTGGGAAACAATTTGGTG+TGG 0.582222 5.4:-8451882 None:intergenic
TCAAGCTCGAGAACATGTAA+TGG 0.585662 5.4:+8452192 MS.gene015300:CDS
TACAAGTTCAGGCACATAGG+TGG 0.586428 5.4:-8451544 None:intergenic
GAGATGCAAGAGAATCCATC+GGG 0.590902 5.4:-8451814 None:intergenic
GCTTACAAGTTCAGGCACAT+AGG 0.592149 5.4:-8451547 None:intergenic
CTCATCTTGATGATCAAACA+AGG 0.597867 5.4:+8451909 MS.gene015300:CDS
CATCAAGATGAGTTTCAACT+GGG 0.598938 5.4:-8451898 None:intergenic
TCTCCTGGGAAGCATACGAC+AGG 0.600421 5.4:+8453883 MS.gene015300:CDS
GATGGACAAAGCTTCTGTGT+TGG 0.607222 5.4:+8452912 MS.gene015300:intron
GTGACAGCAAAGGATCTCCT+GGG 0.607386 5.4:+8453869 MS.gene015300:CDS
AGATCTCTTCACTTGAACTG+TGG 0.609751 5.4:-8453019 None:intergenic
GGAAAACTGTGTGACAGCAA+AGG 0.624039 5.4:+8453859 MS.gene015300:CDS
GGAGATGCAAGAGAATCCAT+CGG 0.627162 5.4:-8451815 None:intergenic
TGTTCAAAAGAAATGAGTTG+AGG 0.643051 5.4:-8451980 None:intergenic
AAGTTCAGGCACATAGGTGG+GGG 0.650925 5.4:-8451541 None:intergenic
AATGGCCGAAAGAAAACGAA+GGG 0.657033 5.4:+8452210 MS.gene015300:CDS
TAATTGGCCAAGCACCGTAG+CGG 0.678187 5.4:-8453175 None:intergenic
AGCACGTTTGCAGACACTGG+AGG 0.686426 5.4:+8452966 MS.gene015300:CDS
ATAACCAAGTGAATCAGACC+AGG 0.687464 5.4:+8452161 MS.gene015300:CDS
TAATGGCCGAAAGAAAACGA+AGG 0.695210 5.4:+8452209 MS.gene015300:CDS
ATTCAATGTGACAAACACAG+TGG 0.762327 5.4:+8453147 MS.gene015300:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAAATTAACATTTCTA+AGG + chr5.4:8453349-8453368 MS.gene015300:intron 10.0%
!! AATTAATGTATTCAATATTA+TGG + chr5.4:8452881-8452900 MS.gene015300:intron 10.0%
!!! AAAAAATCATAAATTTTAGA+CGG + chr5.4:8453592-8453611 MS.gene015300:intron 10.0%
!!! TCAATAATTTATTTTTCTTT+TGG + chr5.4:8452465-8452484 MS.gene015300:intron 10.0%
!! TACAATATAAATATTGCAAT+TGG - chr5.4:8452844-8452863 None:intergenic 15.0%
!! TATAGGAAAATATTACATAT+AGG + chr5.4:8453312-8453331 MS.gene015300:intron 15.0%
!! TTTATGAATTATGATAATCT+AGG - chr5.4:8453814-8453833 None:intergenic 15.0%
!!! AAAATTTGAAATGAAAAATG+TGG + chr5.4:8453615-8453634 MS.gene015300:intron 15.0%
!!! AAGTTTATGAATTTTAAGAT+AGG - chr5.4:8451588-8451607 None:intergenic 15.0%
!!! TTGTTTTTATTTCATTTTCT+AGG + chr5.4:8452667-8452686 MS.gene015300:intron 15.0%
!! ACATTAATGATCCATATTAA+AGG + chr5.4:8452375-8452394 MS.gene015300:intron 20.0%
!! CCACAAAAAAAAAAAAAAAG+AGG - chr5.4:8453643-8453662 None:intergenic 20.0%
!!! CCTCTTTTTTTTTTTTTTTG+TGG + chr5.4:8453640-8453659 MS.gene015300:intron 20.0%
!!! CTTTTTTTTTTTTTTTGTGG+TGG + chr5.4:8453643-8453662 MS.gene015300:intron 20.0%
!!! TATTTTCTTATGATCAGATT+AGG - chr5.4:8452530-8452549 None:intergenic 20.0%
!!! TCTTTAATTACTTTTGAACA+GGG + chr5.4:8451761-8451780 MS.gene015300:intron 20.0%
! ATTATAACATGTTTGTGAGT+TGG - chr5.4:8452306-8452325 None:intergenic 25.0%
! GCATACTTGAACATAATAAA+CGG + chr5.4:8453510-8453529 MS.gene015300:intron 25.0%
! GTAGTATGTTGATATAGAAA+TGG - chr5.4:8452344-8452363 None:intergenic 25.0%
! TAAAATTGCTTAGAAGCAAT+TGG - chr5.4:8452019-8452038 None:intergenic 25.0%
! TATTGACGAAAAACATGAAA+TGG - chr5.4:8452451-8452470 None:intergenic 25.0%
! TGATATAGAAATGGTTTGAA+TGG - chr5.4:8452335-8452354 None:intergenic 25.0%
! TTGGTAAGTTCAACTTAATT+AGG - chr5.4:8452825-8452844 None:intergenic 25.0%
! TTTATTTACCTTAGCAACAA+TGG - chr5.4:8453268-8453287 None:intergenic 25.0%
!! TGAGATTAAGGTTTTCTAAT+TGG - chr5.4:8453194-8453213 None:intergenic 25.0%
!! TTAGTCTTCGAGAAAAAAAT+AGG + chr5.4:8452560-8452579 MS.gene015300:intron 25.0%
!!! CTCTTTAATTACTTTTGAAC+AGG + chr5.4:8451760-8451779 MS.gene015300:intron 25.0%
!!! TAACATTTCTAAGGTTGTTT+AGG + chr5.4:8453358-8453377 MS.gene015300:intron 25.0%
AAAACCAGACAATTATGACA+TGG + chr5.4:8453392-8453411 MS.gene015300:intron 30.0%
AAATGGTCACTAATATTGCT+AGG - chr5.4:8452434-8452453 None:intergenic 30.0%
AAGAGTAGACATCAAAAAGT+TGG + chr5.4:8452606-8452625 MS.gene015300:intron 30.0%
AATATTATGGTATGTGCAGA+TGG + chr5.4:8452894-8452913 MS.gene015300:intron 30.0%
AATGGTCACTAATATTGCTA+GGG - chr5.4:8452433-8452452 None:intergenic 30.0%
AGAGTAGACATCAAAAAGTT+GGG + chr5.4:8452607-8452626 MS.gene015300:intron 30.0%
AGTAATGTTCTACTTACACT+AGG + chr5.4:8452738-8452757 MS.gene015300:intron 30.0%
ATCCATATTAAAGGCTGAAA+TGG + chr5.4:8452384-8452403 MS.gene015300:intron 30.0%
CCAAAGTTGTCGAATAATAT+TGG - chr5.4:8451839-8451858 None:intergenic 30.0%
CCAATATTATTCGACAACTT+TGG + chr5.4:8451836-8451855 MS.gene015300:CDS 30.0%
TAAAACCTAAAGTCGAGAAA+AGG + chr5.4:8452059-8452078 MS.gene015300:CDS 30.0%
TGTTCAAAAGAAATGAGTTG+AGG - chr5.4:8451983-8452002 None:intergenic 30.0%
! GATAAGATCATACCTTTTTG+AGG - chr5.4:8452282-8452301 None:intergenic 30.0%
! GTTTTACATCGGAATAATCA+AGG - chr5.4:8452045-8452064 None:intergenic 30.0%
!! AATTACTTTTGAACAGGGAA+TGG + chr5.4:8451766-8451785 MS.gene015300:intron 30.0%
!! ATCAACAATGTTGTTTCCAA+AGG - chr5.4:8453241-8453260 None:intergenic 30.0%
!! TCAACAATGTTGTTTCCAAA+GGG - chr5.4:8453240-8453259 None:intergenic 30.0%
!! TTATTTTTCTGGTCGAACTT+TGG + chr5.4:8451693-8451712 MS.gene015300:intron 30.0%
!!! TATCGATGTTTTGATCTTCT+TGG - chr5.4:8451939-8451958 None:intergenic 30.0%
!!! TCATTTTCTAGGATCAAATC+AGG + chr5.4:8452678-8452697 MS.gene015300:intron 30.0%
!!! TTGACCAAGCTTTATTTTTC+TGG + chr5.4:8451682-8451701 MS.gene015300:intron 30.0%
AATTCTCTTGCAGATGAACA+AGG + chr5.4:8453838-8453857 MS.gene015300:intron 35.0%
AGTAGTATGCTTACAAGTTC+AGG - chr5.4:8451558-8451577 None:intergenic 35.0%
ATTCAATGTGACAAACACAG+TGG + chr5.4:8453147-8453166 MS.gene015300:CDS 35.0%
CACCATTTCAGCCTTTAATA+TGG - chr5.4:8452389-8452408 None:intergenic 35.0%
CATCAAGATGAGTTTCAACT+GGG - chr5.4:8451901-8451920 None:intergenic 35.0%
GAGTAGACATCAAAAAGTTG+GGG + chr5.4:8452608-8452627 MS.gene015300:intron 35.0%
GATACTCTTACTTCGATTTC+AGG - chr5.4:8453105-8453124 None:intergenic 35.0%
GCTGAAGACAACAAAAAAAC+TGG + chr5.4:8452994-8453013 MS.gene015300:CDS 35.0%
GTTTCAACTGGGAAACAATT+TGG - chr5.4:8451890-8451909 None:intergenic 35.0%
TACTTTGGACAGTGAGATTA+AGG - chr5.4:8453206-8453225 None:intergenic 35.0%
TCAGCAACGAAAATTAGTGA+CGG - chr5.4:8453730-8453749 None:intergenic 35.0%
TCATCAAGATGAGTTTCAAC+TGG - chr5.4:8451902-8451921 None:intergenic 35.0%
TCGACCAGAAAAATAAAGCT+TGG - chr5.4:8451689-8451708 None:intergenic 35.0%
TGATGTAACCATTGTTGCTA+AGG + chr5.4:8453257-8453276 MS.gene015300:CDS 35.0%
! AATGAGTTGAGGTGATGAAT+AGG - chr5.4:8451972-8451991 None:intergenic 35.0%
! CAAAGATTTTCTTCCAGCTT+TGG + chr5.4:8452093-8452112 MS.gene015300:CDS 35.0%
! CTCATCTTGATGATCAAACA+AGG + chr5.4:8451909-8451928 MS.gene015300:CDS 35.0%
! CTTTTCCCTTCGTTTTCTTT+CGG - chr5.4:8452218-8452237 None:intergenic 35.0%
! TTACACCTTTTCTCGACTTT+AGG - chr5.4:8452067-8452086 None:intergenic 35.0%
!! CTCGACTTTAGGTTTTACAT+CGG - chr5.4:8452056-8452075 None:intergenic 35.0%
!!! GATGTTTTGATCTTCTTGGT+TGG - chr5.4:8451935-8451954 None:intergenic 35.0%
AATGGCCGAAAGAAAACGAA+GGG + chr5.4:8452210-8452229 MS.gene015300:CDS 40.0%
ACGACCATGTCATAATTGTC+TGG - chr5.4:8453399-8453418 None:intergenic 40.0%
AGATCTCTTCACTTGAACTG+TGG - chr5.4:8453022-8453041 None:intergenic 40.0%
ATAACCAAGTGAATCAGACC+AGG + chr5.4:8452161-8452180 MS.gene015300:CDS 40.0%
ATGGACAAAGCTTCTGTGTT+GGG + chr5.4:8452913-8452932 MS.gene015300:CDS 40.0%
CAGCAACGAAAATTAGTGAC+GGG - chr5.4:8453729-8453748 None:intergenic 40.0%
CTGAAAGAGCGATGAAACTT+CGG - chr5.4:8452246-8452265 None:intergenic 40.0%
CTTACTTCGATTTCAGGAAG+TGG - chr5.4:8453099-8453118 None:intergenic 40.0%
CTTCTTTAACCAATACCCGA+TGG + chr5.4:8451799-8451818 MS.gene015300:CDS 40.0%
GAGGTGATGAATAGGAAGAA+GGG - chr5.4:8451964-8451983 None:intergenic 40.0%
GTGTGATCTCTCTAATTCTC+TGG + chr5.4:8452406-8452425 MS.gene015300:intron 40.0%
TAATGGCCGAAAGAAAACGA+AGG + chr5.4:8452209-8452228 MS.gene015300:CDS 40.0%
TCAAGCTCGAGAACATGTAA+TGG + chr5.4:8452192-8452211 MS.gene015300:CDS 40.0%
TGAAAGAGCGATGAAACTTC+GGG - chr5.4:8452245-8452264 None:intergenic 40.0%
TGAGGTGATGAATAGGAAGA+AGG - chr5.4:8451965-8451984 None:intergenic 40.0%
TTAGTGAGATGTTCCAACCT+CGG - chr5.4:8453669-8453688 None:intergenic 40.0%
! AACTGGGAAACAATTTGGTG+TGG - chr5.4:8451885-8451904 None:intergenic 40.0%
! AGTAGTACCTTTTTGCCCTT+TGG + chr5.4:8453222-8453241 MS.gene015300:CDS 40.0%
! GTTGTTTCCAAAGGGCAAAA+AGG - chr5.4:8453232-8453251 None:intergenic 40.0%
! TACTTTTGAACAGGGAATGG+AGG + chr5.4:8451769-8451788 MS.gene015300:intron 40.0%
! TTCAGGAAGTGGTTGGTTAT+TGG - chr5.4:8453088-8453107 None:intergenic 40.0%
!! AGAAGCAATTGGTGTTGAAC+TGG - chr5.4:8452008-8452027 None:intergenic 40.0%
!! AGGGCAAAAAGGTACTACTT+TGG - chr5.4:8453221-8453240 None:intergenic 40.0%
!!! TTTTTTTTTTGTGGTGGCCG+AGG + chr5.4:8453649-8453668 MS.gene015300:intron 40.0%
ACAAGTTCAGGCACATAGGT+GGG - chr5.4:8451546-8451565 None:intergenic 45.0%
CAAGAGAATCCATCGGGTAT+TGG - chr5.4:8451811-8451830 None:intergenic 45.0%
CATTGTTCCTGGCCTCAAAA+AGG + chr5.4:8452267-8452286 MS.gene015300:CDS 45.0%
CTTCGATTTCAGGAAGTGGT+TGG - chr5.4:8453095-8453114 None:intergenic 45.0%
GAACCTTGAGAAACCAAAGC+TGG - chr5.4:8452109-8452128 None:intergenic 45.0%
GAGATGCAAGAGAATCCATC+GGG - chr5.4:8451817-8451836 None:intergenic 45.0%
GATGGACAAAGCTTCTGTGT+TGG + chr5.4:8452912-8452931 MS.gene015300:intron 45.0%
GCTTACAAGTTCAGGCACAT+AGG - chr5.4:8451550-8451569 None:intergenic 45.0%
GGAAAACTGTGTGACAGCAA+AGG + chr5.4:8453859-8453878 MS.gene015300:CDS 45.0%
GGAGATGCAAGAGAATCCAT+CGG - chr5.4:8451818-8451837 None:intergenic 45.0%
TACAAGTTCAGGCACATAGG+TGG - chr5.4:8451547-8451566 None:intergenic 45.0%
TGTTCCTGGTCTGATTCACT+TGG - chr5.4:8452168-8452187 None:intergenic 45.0%
! CTTCCAGCTTTGGTTTCTCA+AGG + chr5.4:8452103-8452122 MS.gene015300:CDS 45.0%
!!! CTTTTTGAGGCCAGGAACAA+TGG - chr5.4:8452269-8452288 None:intergenic 45.0%
!!! GATCATACCTTTTTGAGGCC+AGG - chr5.4:8452277-8452296 None:intergenic 45.0%
!! TTTAATAATGTATTATATAT+AGG + chr5.4:8453295-8453314 MS.gene015300:intron 5.0%
AAGTTCAGGCACATAGGTGG+GGG - chr5.4:8451544-8451563 None:intergenic 50.0%
ACAAGCACGTTTGCAGACAC+TGG + chr5.4:8452963-8452982 MS.gene015300:CDS 50.0%
CAAGTTCAGGCACATAGGTG+GGG - chr5.4:8451545-8451564 None:intergenic 50.0%
GCTCTTTCAGCCATTGTTCC+TGG + chr5.4:8452256-8452275 MS.gene015300:CDS 50.0%
GTGACAGCAAAGGATCTCCT+GGG + chr5.4:8453869-8453888 MS.gene015300:CDS 50.0%
TGTGACAGCAAAGGATCTCC+TGG + chr5.4:8453868-8453887 MS.gene015300:CDS 50.0%
! TAATTGGCCAAGCACCGTAG+CGG - chr5.4:8453178-8453197 None:intergenic 50.0%
!!! TTTTTTGTGGTGGCCGAGGT+TGG + chr5.4:8453653-8453672 MS.gene015300:intron 50.0%
AAGCCTGTCGTATGCTTCCC+AGG - chr5.4:8453889-8453908 None:intergenic 55.0%
AGCACGTTTGCAGACACTGG+AGG + chr5.4:8452966-8452985 MS.gene015300:CDS 55.0%
TCGAGCTTGAGCAGTGTTCC+TGG - chr5.4:8452182-8452201 None:intergenic 55.0%
! TCTCCTGGGAAGCATACGAC+AGG + chr5.4:8453883-8453902 MS.gene015300:CDS 55.0%
! ACACAGTGGACGTGCCGCTA+CGG + chr5.4:8453161-8453180 MS.gene015300:CDS 60.0%
! GGACGTGCCGCTACGGTGCT+TGG + chr5.4:8453168-8453187 MS.gene015300:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 8451525 8453925 8451525 ID=MS.gene015300
chr5.4 mRNA 8451525 8453925 8451525 ID=MS.gene015300.t1;Parent=MS.gene015300
chr5.4 exon 8451525 8451563 8451525 ID=MS.gene015300.t1.exon1;Parent=MS.gene015300.t1
chr5.4 CDS 8451525 8451563 8451525 ID=cds.MS.gene015300.t1;Parent=MS.gene015300.t1
chr5.4 exon 8451782 8452288 8451782 ID=MS.gene015300.t1.exon2;Parent=MS.gene015300.t1
chr5.4 CDS 8451782 8452288 8451782 ID=cds.MS.gene015300.t1;Parent=MS.gene015300.t1
chr5.4 exon 8452913 8453278 8452913 ID=MS.gene015300.t1.exon3;Parent=MS.gene015300.t1
chr5.4 CDS 8452913 8453278 8452913 ID=cds.MS.gene015300.t1;Parent=MS.gene015300.t1
chr5.4 exon 8453851 8453925 8453851 ID=MS.gene015300.t1.exon4;Parent=MS.gene015300.t1
chr5.4 CDS 8453851 8453925 8453851 ID=cds.MS.gene015300.t1;Parent=MS.gene015300.t1
Gene Sequence

>MS.gene015300

ATGATGCAAATTATGTCCCCCACCTATGTGCCTGAACTTGTAAGCATACTACTATCATCACCTATCTTAAAATTCATAAACTTTAAGTAATCACAATTATAGCTGAACTTGAAGTATTGAAGTGATTCATCTTAAAATCTTAGCTCTCGAATAATTTTTGACCAAGCTTTATTTTTCTGGTCGAACTTTGGATTTGAAGAAATGAAGTTGCATGTGTATAAAAGAAGAAAGCTTACTCTTTAATTACTTTTGAACAGGGAATGGAGGATCTCAACTTCTTTAACCAATACCCGATGGATTCTCTTGCATCTCCAATATTATTCGACAACTTTGGCTTTCACTTTGATGACAACGAAACCACACCAAATTGTTTCCCAGTTGAAACTCATCTTGATGATCAAACAAGGCCAACCAAGAAGATCAAAACATCGATAAACCCTTCTTCCTATTCATCACCTCAACTCATTTCTTTTGAACACTCCAGTTCAACACCAATTGCTTCTAAGCAATTTTATAACCTTGATTATTCCGATGTAAAACCTAAAGTCGAGAAAAGGTGTAATGATAACAAAGATTTTCTTCCAGCTTTGGTTTCTCAAGGTTCTTATGAAGATCAAAAAATATTTTCAAATTATGATAACCAAGTGAATCAGACCAGGAACACTGCTCAAGCTCGAGAACATGTAATGGCCGAAAGAAAACGAAGGGAAAAGCTTACCCGAAGTTTCATCGCTCTTTCAGCCATTGTTCCTGGCCTCAAAAAGGTATGATCTTATCTCCAACTCACAAACATGTTATAATGTAACTCCATTCAAACCATTTCTATATCAACATACTACTAGAAAAAGAAACATTAATGATCCATATTAAAGGCTGAAATGGTGTGATCTCTCTAATTCTCTGGTCCCTAGCAATATTAGTGACCATTTCATGTTTTTCGTCAATAATTTATTTTTCTTTTGGAATGAAGACTTGTTTTAATTTCTGAGAAAAACAGTATAACCTAATCTGATCATAAGAAAATAAAATCAATTTTTAGTCTTCGAGAAAAAAATAGGACAGCTTTTACTACTCAGACACAAAGAGTAGACATCAAAAAGTTGGGGATTAAATTGTTCTAGTTTCTTTTCTCAGAAACTAAATTGTTTTTATTTCATTTTCTAGGATCAAATCAGGTATCTAATTTTTTTATAATTTAAATTTTATAATTGTGAGTAATGTTCTACTTACACTAGGTGCAACTAATTTCAGTAGTACTAAATTACTGATTAGTGAATAAACACACATGCAAGCTTAACCTAATTAAGTTGAACTTACCAATTGCAATATTTATATTGTACTAGAATTGAATGAATAAATTAATGTATTCAATATTATGGTATGTGCAGATGGACAAAGCTTCTGTGTTGGGAGATGCTATCAAGTACATGAAACAACTACAAGCACGTTTGCAGACACTGGAGGAACAAGCTGAAGACAACAAAAAAACTGGATCCACAGTTCAAGTGAAGAGATCTATTATCTTCACTAATGATGACGATGATGATGATGATAGCAACTCCAATAACCAACCACTTCCTGAAATCGAAGTAAGAGTATCAAGCAAAGATGTACTCATCAAGATTCAATGTGACAAACACAGTGGACGTGCCGCTACGGTGCTTGGCCAATTAGAAAACCTTAATCTCACTGTCCAAAGTAGTACCTTTTTGCCCTTTGGAAACAACATTGTTGATGTAACCATTGTTGCTAAGGTAAATAAATTAATAATTTAATAATGTATTATATATAGGAAAATATTACATATAGGACATGTTTGAATTGAAAATAAATTAACATTTCTAAGGTTGTTTAGGAGAGCTTATGAAAAACCAGACAATTATGACATGGTCGTAAATTATTTCAACTTATTTTCATAAACTATTATAGAAAATTTATGAATACAATTTAACTTTATTTTATCTTTTGTTATAGAAATATGAATTGCATACTTGAACATAATAAACGGACTATGTTATAAGCATTTAATGATGCAAGTTTGTATGTGCGTGTCTATATACACACACTAAAAAATCATAAATTTTAGACGGAAAATTTGAAATGAAAAATGTGGAACCTCTTTTTTTTTTTTTTTGTGGTGGCCGAGGTTGGAACATCTCACTAATTTTGTGACTAAAGAATTAGATAGACATTTCAACTTTCCCGTCACTAATTTTCGTTGCTGATTTTTTTTTTTTTAGTGCACATAATTGCATTTTAAATAAATTAATTTATTGAATTGTTAAACCTAGATTATCATAATTCATAAATATTAATTCTCTTGCAGATGAACAAGGAAAACTGTGTGACAGCAAAGGATCTCCTGGGAAGCATACGACAGGCTTTAATCATCCAAAATTAA
Protein sequence

>MS.gene015300.t1

MMQIMSPTYVPELGMEDLNFFNQYPMDSLASPILFDNFGFHFDDNETTPNCFPVETHLDDQTRPTKKIKTSINPSSYSSPQLISFEHSSSTPIASKQFYNLDYSDVKPKVEKRCNDNKDFLPALVSQGSYEDQKIFSNYDNQVNQTRNTAQAREHVMAERKRREKLTRSFIALSAIVPGLKKMDKASVLGDAIKYMKQLQARLQTLEEQAEDNKKTGSTVQVKRSIIFTNDDDDDDDSNSNNQPLPEIEVRVSSKDVLIKIQCDKHSGRAATVLGQLENLNLTVQSSTFLPFGNNIVDVTIVAKMNKENCVTAKDLLGSIRQALIIQN