AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015301

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015301.t1 MTR_5g014600 86.930 329 21 5 1 322 1 314 0.0 553
MS.gene015301.t1 MTR_4g097950 53.086 243 81 7 88 322 12 229 7.03e-63 200
MS.gene015301.t1 MTR_2g010450 41.577 279 123 9 62 322 74 330 3.01e-50 171
MS.gene015301.t1 MTR_4g092700 44.309 246 95 8 86 322 98 310 7.35e-49 166
MS.gene015301.t1 MTR_2g104550 41.200 250 115 8 75 322 72 291 1.31e-46 160
MS.gene015301.t1 MTR_5g014520 41.264 269 111 7 61 322 81 309 2.58e-46 160
MS.gene015301.t1 MTR_5g014560 46.073 191 90 2 132 322 106 283 6.83e-44 152
MS.gene015301.t1 MTR_4g097940 39.850 266 103 11 64 322 90 305 1.08e-43 152
MS.gene015301.t1 MTR_0246s0020 35.694 353 180 12 6 322 13 354 3.26e-43 152
MS.gene015301.t1 MTR_4g097920 40.304 263 106 9 64 322 90 305 3.52e-43 151
MS.gene015301.t1 MTR_4g097920 40.304 263 106 9 64 322 75 290 3.53e-43 151
MS.gene015301.t1 MTR_8g009020 39.407 236 120 7 89 322 88 302 6.77e-43 150
MS.gene015301.t1 MTR_0246s0020 35.411 353 181 12 6 322 13 354 1.54e-42 151
MS.gene015301.t1 MTR_2g104490 41.525 236 112 7 87 322 78 287 2.50e-42 148
MS.gene015301.t1 MTR_2g104490 41.102 236 113 7 87 322 78 287 5.88e-42 148
MS.gene015301.t1 MTR_4g009540 39.431 246 118 5 80 322 93 310 1.33e-41 147
MS.gene015301.t1 MTR_5g014640 36.396 283 132 10 59 322 50 303 2.13e-41 147
MS.gene015301.t1 MTR_4g009540 39.024 246 119 5 80 322 93 310 8.36e-41 145
MS.gene015301.t1 MTR_4g009110 39.113 248 120 7 80 322 108 329 9.00e-41 145
MS.gene015301.t1 MTR_6g047570 33.333 348 161 13 2 322 8 311 3.69e-39 141
MS.gene015301.t1 MTR_0011s0260 44.767 172 80 2 152 322 178 335 5.82e-39 141
MS.gene015301.t1 MTR_0011s0210 44.444 171 82 2 152 322 178 335 1.46e-38 140
MS.gene015301.t1 MTR_0250s0040 37.727 220 120 5 104 322 37 240 3.96e-38 136
MS.gene015301.t1 MTR_0246s0050 39.594 197 93 4 130 322 122 296 2.56e-35 130
MS.gene015301.t1 MTR_4g098035 39.594 197 93 4 130 322 143 317 3.66e-35 130
MS.gene015301.t1 MTR_4g066460 41.117 197 95 4 126 322 129 304 1.93e-34 128
MS.gene015301.t1 MTR_2g104560 41.176 170 79 4 156 322 53 204 5.88e-33 122
MS.gene015301.t1 MTR_4g067010 39.459 185 93 4 141 322 128 296 1.13e-32 124
MS.gene015301.t1 MTR_7g080780 35.169 236 107 5 88 322 105 295 1.22e-32 123
MS.gene015301.t1 MTR_7g080780 35.169 236 107 5 88 322 118 308 1.36e-32 124
MS.gene015301.t1 MTR_8g069740 40.449 178 92 4 145 321 101 265 2.33e-31 119
MS.gene015301.t1 MTR_4g066380 33.233 331 164 12 7 322 13 301 5.35e-31 119
MS.gene015301.t1 MTR_2g104500 36.898 187 89 3 136 322 94 251 1.19e-30 117
MS.gene015301.t1 MTR_8g009010 38.095 168 54 2 156 322 133 251 8.56e-29 113
MS.gene015301.t1 MTR_2g104530 36.970 165 87 1 158 322 106 253 7.47e-28 109
MS.gene015301.t1 MTR_5g014550 43.478 138 57 2 187 320 54 174 4.32e-26 103
MS.gene015301.t1 MTR_2g104650 40.120 167 87 3 157 322 94 248 4.80e-26 105
MS.gene015301.t1 MTR_2g104590 37.640 178 79 5 145 322 58 203 1.31e-24 100
MS.gene015301.t1 MTR_6g047550 32.549 255 118 8 2 224 8 240 3.92e-24 99.8
MS.gene015301.t1 MTR_7g451650 39.286 140 62 2 183 322 60 176 2.00e-20 87.4
MS.gene015301.t1 MTR_7g451650 38.571 140 63 2 183 322 60 176 9.32e-20 85.9
MS.gene015301.t1 MTR_4g009510 37.179 156 73 5 168 322 53 184 7.04e-19 84.0
MS.gene015301.t1 MTR_4g009420 37.143 140 57 2 183 322 68 176 1.13e-18 83.2
MS.gene015301.t1 MTR_4g009420 34.839 155 69 3 168 322 54 176 1.85e-18 82.4
MS.gene015301.t1 MTR_7g050350 37.226 137 63 2 186 322 1 114 3.06e-18 80.1
MS.gene015301.t1 MTR_7g451650 37.226 137 63 2 186 322 1 114 3.06e-18 80.1
MS.gene015301.t1 MTR_7g117670 31.757 148 80 3 157 304 402 528 2.57e-17 82.8
MS.gene015301.t1 MTR_8g027495 27.027 148 87 1 157 304 458 584 1.33e-16 80.9
MS.gene015301.t1 MTR_7g096350 58.065 62 25 1 157 217 355 416 2.45e-16 79.7
MS.gene015301.t1 MTR_1g072320 28.966 145 98 2 157 301 470 609 2.03e-15 77.4
MS.gene015301.t1 MTR_3g498695 29.379 177 112 5 143 316 288 454 1.03e-13 72.0
MS.gene015301.t1 MTR_8g067280 45.833 72 39 0 139 210 444 515 1.04e-13 72.0
MS.gene015301.t1 MTR_3g498695 29.379 177 112 5 143 316 288 454 1.13e-13 71.6
MS.gene015301.t1 MTR_8g024790 28.409 176 106 6 123 295 262 420 1.24e-13 71.6
MS.gene015301.t1 MTR_7g113830 23.196 194 142 2 130 316 90 283 1.87e-13 70.5
MS.gene015301.t1 MTR_5g030430 27.094 203 127 5 123 314 463 655 4.06e-13 70.5
MS.gene015301.t1 MTR_8g098275 22.705 207 128 3 117 303 366 560 5.94e-12 66.6
MS.gene015301.t1 MTR_8g098275 22.705 207 128 3 117 303 273 467 8.18e-12 66.2
MS.gene015301.t1 MTR_1g112680 27.957 186 114 6 150 315 310 495 9.88e-12 65.9
MS.gene015301.t1 MTR_7g110810 44.118 68 38 0 142 209 197 264 1.08e-11 65.5
MS.gene015301.t1 MTR_8g098275 23.116 199 121 3 125 303 217 403 1.09e-11 65.9
MS.gene015301.t1 MTR_8g064700 23.280 189 124 3 132 319 230 398 1.31e-11 65.5
MS.gene015301.t1 MTR_4g057270 27.972 143 85 5 159 298 151 278 1.78e-11 64.7
MS.gene015301.t1 MTR_8g024770 35.366 82 50 1 134 215 259 337 2.01e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015301.t1 AT4G37850 39.877 326 154 14 9 320 6 303 4.28e-48 164
MS.gene015301.t1 AT4G37850 39.877 326 154 14 9 320 6 303 4.28e-48 164
MS.gene015301.t1 AT2G22750 51.366 183 77 4 142 321 108 281 4.70e-42 148
MS.gene015301.t1 AT2G22750 51.366 183 77 4 142 321 108 281 4.77e-42 148
MS.gene015301.t1 AT2G22750 51.366 183 77 4 142 321 136 309 1.17e-41 147
MS.gene015301.t1 AT2G22750 51.366 183 77 4 142 321 136 309 1.20e-41 147
MS.gene015301.t1 AT2G22750 51.366 183 77 4 142 321 132 305 1.32e-41 147
MS.gene015301.t1 AT2G22760 45.562 169 80 3 155 322 117 274 4.85e-41 144
MS.gene015301.t1 AT2G22760 44.970 169 81 3 155 322 117 274 1.81e-40 143
MS.gene015301.t1 AT2G22770 43.195 169 94 1 156 322 131 299 7.61e-35 129
MS.gene015301.t1 AT2G46510 47.297 74 39 0 139 212 377 450 1.41e-16 80.9
MS.gene015301.t1 AT1G32640 31.088 193 108 6 139 321 434 611 2.35e-16 80.1
MS.gene015301.t1 AT4G09820 30.464 151 72 2 153 303 359 476 2.37e-14 73.9
MS.gene015301.t1 AT1G01260 39.241 79 48 0 137 215 413 491 3.68e-14 73.6
MS.gene015301.t1 AT1G01260 39.241 79 48 0 137 215 413 491 3.68e-14 73.6
MS.gene015301.t1 AT1G01260 39.241 79 48 0 137 215 413 491 3.68e-14 73.6
MS.gene015301.t1 AT1G12860 30.178 169 102 6 158 322 268 424 4.61e-14 72.8
MS.gene015301.t1 AT1G12860 29.448 163 100 5 158 316 268 419 7.45e-14 72.4
MS.gene015301.t1 AT4G16430 40.476 84 46 1 131 214 298 377 1.32e-13 71.6
MS.gene015301.t1 AT5G46760 30.263 152 83 4 157 301 415 550 2.28e-13 71.2
MS.gene015301.t1 AT4G17880 27.485 171 107 4 131 301 394 547 1.07e-12 68.9
MS.gene015301.t1 AT2G16910 27.811 169 87 4 158 304 315 470 1.99e-12 68.2
MS.gene015301.t1 AT3G26744 28.070 171 107 5 158 322 308 468 2.35e-12 67.8
MS.gene015301.t1 AT5G57150 29.730 185 112 5 144 318 41 217 2.59e-12 65.9
MS.gene015301.t1 AT3G26744 27.108 166 104 5 158 316 308 463 3.11e-12 67.4
MS.gene015301.t1 AT3G26744 27.108 166 104 5 158 316 308 463 3.11e-12 67.4
MS.gene015301.t1 AT3G26744 27.108 166 104 5 158 316 308 463 3.11e-12 67.4
MS.gene015301.t1 AT5G57150 29.032 186 114 5 144 319 41 218 3.18e-12 65.5
MS.gene015301.t1 AT5G41315 26.144 153 103 3 157 299 446 598 5.92e-12 67.0
MS.gene015301.t1 AT5G41315 26.144 153 103 3 157 299 441 593 6.00e-12 67.0
MS.gene015301.t1 AT5G41315 26.144 153 103 3 157 299 449 601 6.20e-12 66.6
MS.gene015301.t1 AT5G57150 28.649 185 114 5 144 318 40 216 2.12e-11 63.2
MS.gene015301.t1 AT5G57150 28.962 183 112 5 144 316 41 215 2.64e-11 63.5
MS.gene015301.t1 AT3G61950 29.932 147 88 2 157 301 128 261 3.89e-11 63.2
MS.gene015301.t1 AT1G63650 26.174 149 103 3 157 299 405 552 4.68e-11 63.9
MS.gene015301.t1 AT1G63650 26.174 149 103 3 157 299 405 552 4.68e-11 63.9
MS.gene015301.t1 AT1G63650 26.174 149 103 3 157 299 405 552 4.68e-11 63.9
MS.gene015301.t1 AT3G61950 29.932 147 88 2 157 301 128 261 5.36e-11 63.2
MS.gene015301.t1 AT3G61950 29.932 147 88 2 157 301 179 312 5.74e-11 63.2
MS.gene015301.t1 AT3G61950 29.932 147 88 2 157 301 179 312 6.67e-11 63.2
MS.gene015301.t1 AT5G57150 29.121 182 111 5 144 315 40 213 6.77e-11 62.0

Find 50 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATGTGATCTTGAGCATTTC+TGG 0.145443 5.4:-8427331 None:intergenic
AGTAGTTGTGCATTGCTATT+TGG 0.235575 5.4:+8427879 MS.gene015301:CDS
ATGGATAAGGCTTCTGTATT+AGG 0.248877 5.4:+8427528 MS.gene015301:CDS
TTCAACATTGGTTCTAGTTT+TGG 0.266677 5.4:-8427174 None:intergenic
CTCTCTTGAATATTGCGTTT+TGG 0.303171 5.4:-8427288 None:intergenic
TCAGACACCTCATCAAATTC+TGG 0.305371 5.4:+8427681 MS.gene015301:CDS
TGGATAAGGCTTCTGTATTA+GGG 0.314098 5.4:+8427529 MS.gene015301:CDS
ACTCCTTCCAGACTTGAAAA+AGG 0.315242 5.4:+8427410 MS.gene015301:CDS
TTGGTCTTAAGTGTCTTTGT+AGG 0.346951 5.4:-8427066 None:intergenic
GACACTCTTGCCTCAACTTC+AGG 0.353101 5.4:-8427759 None:intergenic
CAGATTCTACGTTGTTCCTT+TGG 0.354402 5.4:-8427611 None:intergenic
GGGAAATCTTTAACCTCAAA+AGG 0.376975 5.4:+8427240 MS.gene015301:CDS
GACAAGTTGGTTGTGTGATT+TGG 0.403546 5.4:+8426802 MS.gene015301:CDS
GATGAAGATTCTCTATCTCT+TGG 0.405403 5.4:-8427845 None:intergenic
CAAAGCAAAGAATGTAAGAA+TGG 0.443123 5.4:-8427134 None:intergenic
GATTCAACTTTGAAACCAAA+AGG 0.445350 5.4:+8427201 MS.gene015301:CDS
GGAATTGCTCATAGTGTTTG+AGG 0.462284 5.4:-8427016 None:intergenic
GTACCCTATGTTTGAAGGGT+TGG 0.464961 5.4:-8427085 None:intergenic
ACTTCAGGCAGTGACCTACT+TGG 0.469914 5.4:-8427744 None:intergenic
GAAGGGTACCCTATGTTTGA+AGG 0.486283 5.4:-8427090 None:intergenic
CATTTGGATGTAGATGGATA+AGG 0.492545 5.4:+8427515 MS.gene015301:intron
AAAGATTGTTGCAGTGACAT+AGG 0.493042 5.4:-8426964 None:intergenic
AAGACCAACCCTTCAAACAT+AGG 0.497958 5.4:+8427081 MS.gene015301:CDS
AAGATTGTTGCAGTGACATA+GGG 0.498647 5.4:-8426963 None:intergenic
ATATCATACCTCAGCAATGA+TGG 0.498759 5.4:-8427922 None:intergenic
TCACTGCAACAATCTTTCTC+TGG 0.504710 5.4:+8426970 MS.gene015301:CDS
AAAGCAAAGAATGTAAGAAT+GGG 0.507449 5.4:-8427133 None:intergenic
GTTGAATCAATGTTCAACAT+TGG 0.511485 5.4:-8427186 None:intergenic
ACCTCATCAAATTCTGGCTA+TGG 0.514888 5.4:+8427687 MS.gene015301:CDS
TTTCTTGAAGCTGTTTCACA+TGG 0.520049 5.4:-8427563 None:intergenic
AACTTTGAAACCAAAAGGCA+AGG 0.533663 5.4:+8427206 MS.gene015301:CDS
CACTCTTATGTTTCTCACAA+TGG 0.534111 5.4:-8427806 None:intergenic
AACACTATGAGCAATTCCTC+AGG 0.557225 5.4:+8427021 MS.gene015301:CDS
GGAAATCTTTAACCTCAAAA+GGG 0.559810 5.4:+8427241 MS.gene015301:CDS
TGACATCAAACGATGAGTCA+TGG 0.561998 5.4:+8426780 MS.gene015301:CDS
AGACCAACCCTTCAAACATA+GGG 0.575956 5.4:+8427082 MS.gene015301:CDS
AAGGGTACCCTATGTTTGAA+GGG 0.581486 5.4:-8427089 None:intergenic
ACGATGAGTCATGGACAAGT+TGG 0.581739 5.4:+8426789 MS.gene015301:CDS
TAGGTCACTGCCTGAAGTTG+AGG 0.613765 5.4:+8427749 MS.gene015301:CDS
CTGCAACAATCTTTCTCTGG+TGG 0.629704 5.4:+8426973 MS.gene015301:CDS
TTGAGGTTGTGTGAGAGCAA+TGG 0.632047 5.4:-8426999 None:intergenic
AAGGAACAACGTAGAATCTG+TGG 0.639673 5.4:+8427614 MS.gene015301:CDS
GAGATGTTAACTGCTTTGAG+AGG 0.640656 5.4:+8427043 MS.gene015301:CDS
TATTGGAGGAAAAGAACCAA+AGG 0.643739 5.4:+8427595 MS.gene015301:CDS
ACCATAGCCAGAATTTGATG+AGG 0.651808 5.4:-8427688 None:intergenic
GGATAAGGCTTCTGTATTAG+GGG 0.656232 5.4:+8427530 MS.gene015301:CDS
TGACATAACCATCATTGCTG+AGG 0.668356 5.4:+8427914 MS.gene015301:CDS
CTCACACCTCAAAACCAAGT+AGG 0.668534 5.4:+8427730 MS.gene015301:CDS
AGATTGTTGCAGTGACATAG+GGG 0.673309 5.4:-8426962 None:intergenic
AAAGCAGTTAACATCTCCTG+AGG 0.687587 5.4:-8427037 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAATGATACTAATTAA+TGG + chr5.4:8427480-8427499 MS.gene015301:intron 10.0%
!!! AATTTTGTTTGCACTTTTTT+TGG + chr5.4:8426856-8426875 MS.gene015301:intron 20.0%
! AAAGCAAAGAATGTAAGAAT+GGG - chr5.4:8427136-8427155 None:intergenic 25.0%
! ATGGTTACTCATTAATCATT+TGG + chr5.4:8427499-8427518 MS.gene015301:intron 25.0%
! ATTAATCATTTGGATGTAGA+TGG + chr5.4:8427509-8427528 MS.gene015301:intron 25.0%
! TCAAGAAAAAGTGAAACTAT+TGG + chr5.4:8427578-8427597 MS.gene015301:CDS 25.0%
! TCCTTAGAAAACCAAAAAAA+GGG + chr5.4:8427264-8427283 MS.gene015301:CDS 25.0%
!!! AAATCTTTTTTTGGTAAGGA+AGG - chr5.4:8427111-8427130 None:intergenic 25.0%
!!! AATCTTTTTTTGGTAAGGAA+GGG - chr5.4:8427110-8427129 None:intergenic 25.0%
!!! GAATGGGAAAAATCTTTTTT+TGG - chr5.4:8427120-8427139 None:intergenic 25.0%
!!! GGAAAAATCTTTTTTTGGTA+AGG - chr5.4:8427115-8427134 None:intergenic 25.0%
!!! TTGATGATTTTGTAATCTTC+TGG - chr5.4:8426928-8426947 None:intergenic 25.0%
AAAGTGCAAACAAAATTGTG+TGG - chr5.4:8426853-8426872 None:intergenic 30.0%
AGAAAAAGTGAAACTATTGG+AGG + chr5.4:8427581-8427600 MS.gene015301:CDS 30.0%
CAAAGCAAAGAATGTAAGAA+TGG - chr5.4:8427137-8427156 None:intergenic 30.0%
GGAAATCTTTAACCTCAAAA+GGG + chr5.4:8427241-8427260 MS.gene015301:CDS 30.0%
GTCCTTAGAAAACCAAAAAA+AGG + chr5.4:8427263-8427282 MS.gene015301:CDS 30.0%
GTTGAATCAATGTTCAACAT+TGG - chr5.4:8427189-8427208 None:intergenic 30.0%
! AAAGGCAAGGTTTTTAATCA+CGG + chr5.4:8427219-8427238 MS.gene015301:CDS 30.0%
! TGATTAAAAACCTTGCCTTT+TGG - chr5.4:8427219-8427238 None:intergenic 30.0%
!! GATTCAACTTTGAAACCAAA+AGG + chr5.4:8427201-8427220 MS.gene015301:CDS 30.0%
!!! AATTGAACCTTTTTCAAGTC+TGG - chr5.4:8427420-8427439 None:intergenic 30.0%
!!! TCCCTTTTTTTGGTTTTCTA+AGG - chr5.4:8427268-8427287 None:intergenic 30.0%
!!! TTCAACATTGGTTCTAGTTT+TGG - chr5.4:8427177-8427196 None:intergenic 30.0%
AAAGATTGTTGCAGTGACAT+AGG - chr5.4:8426967-8426986 None:intergenic 35.0%
AAGATTGTTGCAGTGACATA+GGG - chr5.4:8426966-8426985 None:intergenic 35.0%
ATGGATAAGGCTTCTGTATT+AGG + chr5.4:8427528-8427547 MS.gene015301:CDS 35.0%
CACTCTTATGTTTCTCACAA+TGG - chr5.4:8427809-8427828 None:intergenic 35.0%
CATTTGGATGTAGATGGATA+AGG + chr5.4:8427515-8427534 MS.gene015301:intron 35.0%
GATGAAGATTCTCTATCTCT+TGG - chr5.4:8427848-8427867 None:intergenic 35.0%
GGGAAATCTTTAACCTCAAA+AGG + chr5.4:8427240-8427259 MS.gene015301:CDS 35.0%
TATGTGATCTTGAGCATTTC+TGG - chr5.4:8427334-8427353 None:intergenic 35.0%
TATTGGAGGAAAAGAACCAA+AGG + chr5.4:8427595-8427614 MS.gene015301:CDS 35.0%
TGGATAAGGCTTCTGTATTA+GGG + chr5.4:8427529-8427548 MS.gene015301:CDS 35.0%
! AAGGCAAGGTTTTTAATCAC+GGG + chr5.4:8427220-8427239 MS.gene015301:CDS 35.0%
! AGTAGTTGTGCATTGCTATT+TGG + chr5.4:8427879-8427898 MS.gene015301:CDS 35.0%
! CTCTCTTGAATATTGCGTTT+TGG - chr5.4:8427291-8427310 None:intergenic 35.0%
! GATGGTTATGTCAAGTTTTG+TGG - chr5.4:8427907-8427926 None:intergenic 35.0%
! TTTCTTGAAGCTGTTTCACA+TGG - chr5.4:8427566-8427585 None:intergenic 35.0%
!! AACTTTGAAACCAAAAGGCA+AGG + chr5.4:8427206-8427225 MS.gene015301:CDS 35.0%
!! TTGGTCTTAAGTGTCTTTGT+AGG - chr5.4:8427069-8427088 None:intergenic 35.0%
AAACTCTTGTGACTTTCCAC+AGG + chr5.4:8426900-8426919 MS.gene015301:intron 40.0%
AAAGCAGTTAACATCTCCTG+AGG - chr5.4:8427040-8427059 None:intergenic 40.0%
AACACTATGAGCAATTCCTC+AGG + chr5.4:8427021-8427040 MS.gene015301:CDS 40.0%
AAGACCAACCCTTCAAACAT+AGG + chr5.4:8427081-8427100 MS.gene015301:CDS 40.0%
AAGGAACAACGTAGAATCTG+TGG + chr5.4:8427614-8427633 MS.gene015301:CDS 40.0%
ACCATAGCCAGAATTTGATG+AGG - chr5.4:8427691-8427710 None:intergenic 40.0%
ACTCCTTCCAGACTTGAAAA+AGG + chr5.4:8427410-8427429 MS.gene015301:CDS 40.0%
AGACCAACCCTTCAAACATA+GGG + chr5.4:8427082-8427101 MS.gene015301:CDS 40.0%
AGATTGTTGCAGTGACATAG+GGG - chr5.4:8426965-8426984 None:intergenic 40.0%
CAGATTCTACGTTGTTCCTT+TGG - chr5.4:8427614-8427633 None:intergenic 40.0%
GGAATTGCTCATAGTGTTTG+AGG - chr5.4:8427019-8427038 None:intergenic 40.0%
GGATAAGGCTTCTGTATTAG+GGG + chr5.4:8427530-8427549 MS.gene015301:CDS 40.0%
TCACTGCAACAATCTTTCTC+TGG + chr5.4:8426970-8426989 MS.gene015301:CDS 40.0%
TCAGACACCTCATCAAATTC+TGG + chr5.4:8427681-8427700 MS.gene015301:CDS 40.0%
TGACATAACCATCATTGCTG+AGG + chr5.4:8427914-8427933 MS.gene015301:CDS 40.0%
TGACATCAAACGATGAGTCA+TGG + chr5.4:8426780-8426799 MS.gene015301:CDS 40.0%
! ACCTCATCAAATTCTGGCTA+TGG + chr5.4:8427687-8427706 MS.gene015301:CDS 40.0%
! GAGATGTTAACTGCTTTGAG+AGG + chr5.4:8427043-8427062 MS.gene015301:CDS 40.0%
!! AAGGGTACCCTATGTTTGAA+GGG - chr5.4:8427092-8427111 None:intergenic 40.0%
!! GACAAGTTGGTTGTGTGATT+TGG + chr5.4:8426802-8426821 MS.gene015301:CDS 40.0%
!! GTTTTCTAAGGACCCTTTTG+AGG - chr5.4:8427256-8427275 None:intergenic 40.0%
!! TGCGTTTTGGTCCCTTTTTT+TGG - chr5.4:8427278-8427297 None:intergenic 40.0%
!! TTGTAATCTTCTGGTTCCTG+TGG - chr5.4:8426919-8426938 None:intergenic 40.0%
!!! GAACCTTTTTCAAGTCTGGA+AGG - chr5.4:8427416-8427435 None:intergenic 40.0%
ACGATGAGTCATGGACAAGT+TGG + chr5.4:8426789-8426808 MS.gene015301:CDS 45.0%
CTCACACCTCAAAACCAAGT+AGG + chr5.4:8427730-8427749 MS.gene015301:CDS 45.0%
CTGCAACAATCTTTCTCTGG+TGG + chr5.4:8426973-8426992 MS.gene015301:CDS 45.0%
GTACCCTATGTTTGAAGGGT+TGG - chr5.4:8427088-8427107 None:intergenic 45.0%
TTGAGGTTGTGTGAGAGCAA+TGG - chr5.4:8427002-8427021 None:intergenic 45.0%
!! GAAGGGTACCCTATGTTTGA+AGG - chr5.4:8427093-8427112 None:intergenic 45.0%
!!! CAGTGACCTACTTGGTTTTG+AGG - chr5.4:8427739-8427758 None:intergenic 45.0%
ACTTCAGGCAGTGACCTACT+TGG - chr5.4:8427747-8427766 None:intergenic 50.0%
GACACTCTTGCCTCAACTTC+AGG - chr5.4:8427762-8427781 None:intergenic 50.0%
TAGGTCACTGCCTGAAGTTG+AGG + chr5.4:8427749-8427768 MS.gene015301:CDS 50.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 8426779 8427941 8426779 ID=MS.gene015301
chr5.4 mRNA 8426779 8427941 8426779 ID=MS.gene015301.t1;Parent=MS.gene015301
chr5.4 exon 8426779 8426823 8426779 ID=MS.gene015301.t1.exon1;Parent=MS.gene015301.t1
chr5.4 CDS 8426779 8426823 8426779 ID=cds.MS.gene015301.t1;Parent=MS.gene015301.t1
chr5.4 exon 8426922 8427431 8426922 ID=MS.gene015301.t1.exon2;Parent=MS.gene015301.t1
chr5.4 CDS 8426922 8427431 8426922 ID=cds.MS.gene015301.t1;Parent=MS.gene015301.t1
chr5.4 exon 8427528 8427941 8427528 ID=MS.gene015301.t1.exon3;Parent=MS.gene015301.t1
chr5.4 CDS 8427528 8427941 8427528 ID=cds.MS.gene015301.t1;Parent=MS.gene015301.t1
Gene Sequence

>MS.gene015301

ATGACATCAAACGATGAGTCATGGACAAGTTGGTTGTGTGATTTGGTAAATAAAATTTTGATTTTATTCAACCACACAATTTTGTTTGCACTTTTTTTGGATCTTTTCATTTATTTTACATAAACTCTTGTGACTTTCCACAGGAACCAGAAGATTACAAAATCATCAATGACATTAACATAGCCCCTATGTCACTGCAACAATCTTTCTCTGGTGGTAGCCATTGCTCTCACACAACCTCAAACACTATGAGCAATTCCTCAGGAGATGTTAACTGCTTTGAGAGGCCTACAAAGACACTTAAGACCAACCCTTCAAACATAGGGTACCCTTCCTTACCAAAAAAAGATTTTTCCCATTCTTACATTCTTTGCTTTGACAATGAGAATCAAGAACCAAAACTAGAACCAATGTTGAACATTGATTCAACTTTGAAACCAAAAGGCAAGGTTTTTAATCACGGGAAATCTTTAACCTCAAAAGGGTCCTTAGAAAACCAAAAAAAGGGACCAAAACGCAATATTCAAGAGAGCAAGAAGACTGATTCAGCTGCCAGAAATGCTCAAGATCACATACTTGCTGAGAGAAAGAGAAGAGAAAAAATTAGTCAAAAGTTCATAGCTCTTTCAGCACTCCTTCCAGACTTGAAAAAGGTTCAATTTATTTTTACTCAAATTAATTTTACTCTTTATTTGTGAAACAAATTAATGATACTAATTAATGGTTACTCATTAATCATTTGGATGTAGATGGATAAGGCTTCTGTATTAGGGGATGCTATAAACCATGTGAAACAGCTTCAAGAAAAAGTGAAACTATTGGAGGAAAAGAACCAAAGGAACAACGTAGAATCTGTGGTATATGTTGAGAAGACAAAGTCATGTTCTTCAGATGAAGATGTTTCAGACACCTCATCAAATTCTGGCTATGGTAATTGTTGTCACACTCACACTCACACCTCAAAACCAAGTAGGTCACTGCCTGAAGTTGAGGCAAGAGTGTCAGAGAAAAACGTGCTGATTCGTGTCCATTGTGAGAAACATAAGAGTGTATTGATGAACATAATCCAAGAGATAGAGAATCTTCATCTATCAGTTACTAGTAGTTGTGCATTGCTATTTGGAACCACAAAACTTGACATAACCATCATTGCTGAGGTATGA
Protein sequence

>MS.gene015301.t1

MTSNDESWTSWLCDLEPEDYKIINDINIAPMSLQQSFSGGSHCSHTTSNTMSNSSGDVNCFERPTKTLKTNPSNIGYPSLPKKDFSHSYILCFDNENQEPKLEPMLNIDSTLKPKGKVFNHGKSLTSKGSLENQKKGPKRNIQESKKTDSAARNAQDHILAERKRREKISQKFIALSALLPDLKKMDKASVLGDAINHVKQLQEKVKLLEEKNQRNNVESVVYVEKTKSCSSDEDVSDTSSNSGYGNCCHTHTHTSKPSRSLPEVEARVSEKNVLIRVHCEKHKSVLMNIIQEIENLHLSVTSSCALLFGTTKLDITIIAEV