Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015369.t1 | XP_003611394.3 | 94.3 | 175 | 10 | 0 | 1 | 175 | 1 | 175 | 8.90E-89 | 336.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015369.t1 | Q84R94 | 42.0 | 162 | 76 | 4 | 17 | 161 | 54 | 214 | 1.5e-20 | 100.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015369.t1 | A0A396HMK7 | 94.3 | 175 | 10 | 0 | 1 | 175 | 1 | 175 | 6.4e-89 | 336.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene015369.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015369.t1 | MTR_5g013530 | 87.079 | 178 | 17 | 2 | 1 | 177 | 1 | 173 | 7.39e-113 | 318 |
| MS.gene015369.t1 | MTR_5g013520 | 93.151 | 146 | 10 | 0 | 30 | 175 | 1 | 146 | 8.81e-101 | 286 |
| MS.gene015369.t1 | MTR_5g013530 | 86.000 | 150 | 15 | 2 | 29 | 177 | 7 | 151 | 3.43e-90 | 260 |
| MS.gene015369.t1 | MTR_5g013515 | 72.464 | 138 | 30 | 3 | 31 | 161 | 1 | 137 | 8.25e-68 | 203 |
| MS.gene015369.t1 | MTR_2g042900 | 44.785 | 163 | 43 | 6 | 25 | 156 | 99 | 245 | 7.47e-34 | 120 |
| MS.gene015369.t1 | MTR_2g042900 | 34.132 | 167 | 40 | 5 | 25 | 130 | 99 | 256 | 7.35e-19 | 81.3 |
| MS.gene015369.t1 | MTR_4g124960 | 34.146 | 164 | 68 | 6 | 5 | 162 | 73 | 202 | 4.02e-18 | 78.2 |
| MS.gene015369.t1 | MTR_4g124960 | 33.824 | 136 | 51 | 5 | 5 | 135 | 73 | 174 | 1.43e-14 | 68.2 |
| MS.gene015369.t1 | MTR_8g021380 | 31.538 | 130 | 79 | 3 | 19 | 138 | 79 | 208 | 9.17e-14 | 67.4 |
| MS.gene015369.t1 | MTR_4g124950 | 34.058 | 138 | 65 | 5 | 5 | 141 | 7 | 119 | 4.38e-13 | 63.2 |
| MS.gene015369.t1 | MTR_2g044910 | 26.724 | 116 | 70 | 1 | 40 | 140 | 11 | 126 | 1.28e-11 | 60.1 |
| MS.gene015369.t1 | MTR_2g044910 | 26.724 | 116 | 70 | 1 | 40 | 140 | 54 | 169 | 2.00e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015369.t1 | AT1G19180 | 39.850 | 133 | 69 | 4 | 37 | 159 | 53 | 184 | 5.56e-25 | 95.9 |
| MS.gene015369.t1 | AT1G19180 | 39.850 | 133 | 69 | 4 | 37 | 159 | 119 | 250 | 1.65e-24 | 96.3 |
| MS.gene015369.t1 | AT1G19180 | 39.850 | 133 | 69 | 4 | 37 | 159 | 119 | 250 | 1.65e-24 | 96.3 |
| MS.gene015369.t1 | AT1G74950 | 39.855 | 138 | 64 | 4 | 37 | 157 | 112 | 247 | 1.16e-23 | 94.0 |
| MS.gene015369.t1 | AT1G74950 | 40.909 | 110 | 49 | 2 | 37 | 130 | 112 | 221 | 1.03e-18 | 80.5 |
| MS.gene015369.t1 | AT5G20900 | 32.168 | 143 | 69 | 5 | 24 | 141 | 28 | 167 | 3.75e-15 | 70.1 |
| MS.gene015369.t1 | AT1G17380 | 35.246 | 122 | 65 | 2 | 33 | 140 | 58 | 179 | 3.07e-14 | 68.9 |
| MS.gene015369.t1 | AT1G17380 | 35.246 | 122 | 65 | 2 | 33 | 140 | 87 | 208 | 5.99e-14 | 68.2 |
Find 39 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTACAAGAGATTCCTATTT+TGG | 0.046444 | 5.4:+7383696 | MS.gene015369:CDS |
| CCATCTATACCTGTTATTTA+TGG | 0.164541 | 5.4:+7384096 | MS.gene015369:CDS |
| CTTGGATTGCTTGTTTGATA+TGG | 0.217861 | 5.4:-7384713 | None:intergenic |
| ATTGGTTTATTAATGCTTCT+TGG | 0.268448 | 5.4:-7384731 | None:intergenic |
| AGCTTCACTTCATCGGTTCT+TGG | 0.292747 | 5.4:+7384246 | MS.gene015369:CDS |
| CATGTCATGGATTCATCAAT+TGG | 0.320863 | 5.4:-7384749 | None:intergenic |
| CCAAAATAGGAATCTCTTGT+AGG | 0.331403 | 5.4:-7383696 | None:intergenic |
| ATCCATGACATGGCTCTCTT+TGG | 0.358156 | 5.4:+7384760 | MS.gene015369:CDS |
| TACCTGGAGTTTCCCAAAAT+AGG | 0.395618 | 5.4:-7383709 | None:intergenic |
| GGAAGTTGTTGAAAATTCAT+AGG | 0.405668 | 5.4:-7383664 | None:intergenic |
| GGAAAAGTCCATGATGTCTT+TGG | 0.411671 | 5.4:-7383977 | None:intergenic |
| AGTGAAGCTTTCCTCGTCAT+TGG | 0.416178 | 5.4:-7384232 | None:intergenic |
| ATGTCTTTGGCTTTATCAGC+TGG | 0.417227 | 5.4:-7383964 | None:intergenic |
| TGAAGTACTAGTAATTCCTT+TGG | 0.431697 | 5.4:-7384001 | None:intergenic |
| CAAATGACAATCTTCTATGA+TGG | 0.453411 | 5.4:+7383916 | MS.gene015369:CDS |
| TTCATCGGTTCTTGGAGAAA+AGG | 0.463618 | 5.4:+7384254 | MS.gene015369:CDS |
| CCATAAATAACAGGTATAGA+TGG | 0.477854 | 5.4:-7384096 | None:intergenic |
| GCTTGTTTGATATGGTGCCC+TGG | 0.478945 | 5.4:-7384705 | None:intergenic |
| TCGTAGCCAGAATTGCAGCC+AGG | 0.485781 | 5.4:+7384687 | MS.gene015369:intron |
| AGTACTAGTAATTCCTTTGG+TGG | 0.499055 | 5.4:-7383998 | None:intergenic |
| AATGAAGGCTAATGTTAAGA+AGG | 0.511094 | 5.4:+7383876 | MS.gene015369:CDS |
| CTACACTCAGATTTGTGTTG+TGG | 0.517732 | 5.4:-7384794 | None:intergenic |
| CGAGGAAAGCTTCACTTCAT+CGG | 0.519369 | 5.4:+7384239 | MS.gene015369:CDS |
| AGTAAGCATAGTTGTTGTTG+TGG | 0.527440 | 5.4:-7384029 | None:intergenic |
| AAATGACAATCTTCTATGAT+GGG | 0.538308 | 5.4:+7383917 | MS.gene015369:CDS |
| GATTTGTGTTGTGGTGATTG+TGG | 0.542480 | 5.4:-7384785 | None:intergenic |
| ATCTCTTGTAGGAAGAGATG+AGG | 0.564072 | 5.4:-7383685 | None:intergenic |
| TCTTGGAGAAAAGGAAGGAT+AGG | 0.567398 | 5.4:+7384263 | MS.gene015369:CDS |
| CGTAGCCAGAATTGCAGCCA+GGG | 0.577260 | 5.4:+7384688 | MS.gene015369:intron |
| GGCTAATGTTAAGAAGGAAG+AGG | 0.580916 | 5.4:+7383882 | MS.gene015369:CDS |
| TGCCAAAGAGAGCCATGTCA+TGG | 0.583954 | 5.4:-7384762 | None:intergenic |
| TGATAAAGCCAAAGACATCA+TGG | 0.585378 | 5.4:+7383969 | MS.gene015369:CDS |
| TCGGTTCTTGGAGAAAAGGA+AGG | 0.585705 | 5.4:+7384258 | MS.gene015369:CDS |
| TTAAAGTAACACATGGGTGG+AGG | 0.612419 | 5.4:-7384822 | None:intergenic |
| CAATTGATGAATCCATGACA+TGG | 0.622850 | 5.4:+7384750 | MS.gene015369:CDS |
| AAAACAGTTAAAGTAACACA+TGG | 0.639241 | 5.4:-7384829 | None:intergenic |
| CAGTTAAAGTAACACATGGG+TGG | 0.664883 | 5.4:-7384825 | None:intergenic |
| ATTCAGATTTACCAATGACG+AGG | 0.687022 | 5.4:+7384221 | MS.gene015369:intron |
| AAACAGTTAAAGTAACACAT+GGG | 0.695262 | 5.4:-7384828 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAATTAAATGTAAGTT+TGG | + | chr5.4:7384368-7384387 | MS.gene015369:intron | 10.0% |
| !!! | AAAATTGATTTTGAATAAAT+TGG | - | chr5.4:7384612-7384631 | None:intergenic | 10.0% |
| !!! | TATTTATGGTAAATTTTTTT+TGG | + | chr5.4:7384110-7384129 | MS.gene015369:intron | 10.0% |
| !! | AAAAATTTACCATAAATAAC+AGG | - | chr5.4:7384108-7384127 | None:intergenic | 15.0% |
| !!! | GTTAATAAACTTCACTTTTT+GGG | + | chr5.4:7384318-7384337 | MS.gene015369:intron | 20.0% |
| !!! | TGTTAATAAACTTCACTTTT+TGG | + | chr5.4:7384317-7384336 | MS.gene015369:intron | 20.0% |
| !!! | TTAATAAACTTCACTTTTTG+GGG | + | chr5.4:7384319-7384338 | MS.gene015369:intron | 20.0% |
| ! | AAACATGTCAAAATCAATCT+TGG | - | chr5.4:7384411-7384430 | None:intergenic | 25.0% |
| ! | AAATGACAATCTTCTATGAT+GGG | + | chr5.4:7383917-7383936 | MS.gene015369:CDS | 25.0% |
| ! | AATGTACAGTGATTTATGTT+TGG | - | chr5.4:7384552-7384571 | None:intergenic | 25.0% |
| ! | CATACTAGTAGTATATAACA+TGG | + | chr5.4:7383808-7383827 | MS.gene015369:intron | 25.0% |
| !! | ATTGGTTTATTAATGCTTCT+TGG | - | chr5.4:7384734-7384753 | None:intergenic | 25.0% |
| !! | TGGATATTTTATGTTTGAGA+GGG | + | chr5.4:7384130-7384149 | MS.gene015369:intron | 25.0% |
| !! | TTGGATATTTTATGTTTGAG+AGG | + | chr5.4:7384129-7384148 | MS.gene015369:intron | 25.0% |
| !!! | GTTTTATTACAGTGTAATGA+AGG | + | chr5.4:7383861-7383880 | MS.gene015369:intron | 25.0% |
| AAAAAGAACATGTATGTACC+TGG | - | chr5.4:7383728-7383747 | None:intergenic | 30.0% | |
| AATCTTGGATTATCCAAAAG+TGG | - | chr5.4:7384396-7384415 | None:intergenic | 30.0% | |
| AATGAAGGCTAATGTTAAGA+AGG | + | chr5.4:7383876-7383895 | MS.gene015369:CDS | 30.0% | |
| ACATTCAACTCAATCACTAA+AGG | + | chr5.4:7384567-7384586 | MS.gene015369:intron | 30.0% | |
| CAAAATCAATTCAACTGATG+CGG | - | chr5.4:7384438-7384457 | None:intergenic | 30.0% | |
| CAAATGACAATCTTCTATGA+TGG | + | chr5.4:7383916-7383935 | MS.gene015369:CDS | 30.0% | |
| CCATAAATAACAGGTATAGA+TGG | - | chr5.4:7384099-7384118 | None:intergenic | 30.0% | |
| GGAAGTTGTTGAAAATTCAT+AGG | - | chr5.4:7383667-7383686 | None:intergenic | 30.0% | |
| ! | CCATCTATACCTGTTATTTA+TGG | + | chr5.4:7384096-7384115 | MS.gene015369:CDS | 30.0% |
| ! | CTACAAGAGATTCCTATTTT+GGG | + | chr5.4:7383697-7383716 | MS.gene015369:CDS | 30.0% |
| ! | TGAAGTACTAGTAATTCCTT+TGG | - | chr5.4:7384004-7384023 | None:intergenic | 30.0% |
| AGTACTAGTAATTCCTTTGG+TGG | - | chr5.4:7384001-7384020 | None:intergenic | 35.0% | |
| ATTCAGATTTACCAATGACG+AGG | + | chr5.4:7384221-7384240 | MS.gene015369:intron | 35.0% | |
| CAATTGATGAATCCATGACA+TGG | + | chr5.4:7384750-7384769 | MS.gene015369:CDS | 35.0% | |
| CATGTCATGGATTCATCAAT+TGG | - | chr5.4:7384752-7384771 | None:intergenic | 35.0% | |
| CCAAAATAGGAATCTCTTGT+AGG | - | chr5.4:7383699-7383718 | None:intergenic | 35.0% | |
| CTTGGATTGCTTGTTTGATA+TGG | - | chr5.4:7384716-7384735 | None:intergenic | 35.0% | |
| TGATAAAGCCAAAGACATCA+TGG | + | chr5.4:7383969-7383988 | MS.gene015369:CDS | 35.0% | |
| ! | AGTAAGCATAGTTGTTGTTG+TGG | - | chr5.4:7384032-7384051 | None:intergenic | 35.0% |
| ! | CCTACAAGAGATTCCTATTT+TGG | + | chr5.4:7383696-7383715 | MS.gene015369:CDS | 35.0% |
| ! | GTTTCAAGTAGAATCCATTC+TGG | - | chr5.4:7384464-7384483 | None:intergenic | 35.0% |
| !! | AATTGATTTTGCCTCCAGAA+TGG | + | chr5.4:7384447-7384466 | MS.gene015369:intron | 35.0% |
| !! | TGTAAGTTTGGTTCCACTTT+TGG | + | chr5.4:7384380-7384399 | MS.gene015369:intron | 35.0% |
| ATCTCTTGTAGGAAGAGATG+AGG | - | chr5.4:7383688-7383707 | None:intergenic | 40.0% | |
| CTACACTCAGATTTGTGTTG+TGG | - | chr5.4:7384797-7384816 | None:intergenic | 40.0% | |
| GATTTGTGTTGTGGTGATTG+TGG | - | chr5.4:7384788-7384807 | None:intergenic | 40.0% | |
| GGAAAAGTCCATGATGTCTT+TGG | - | chr5.4:7383980-7383999 | None:intergenic | 40.0% | |
| GGCTAATGTTAAGAAGGAAG+AGG | + | chr5.4:7383882-7383901 | MS.gene015369:CDS | 40.0% | |
| TACCTGGAGTTTCCCAAAAT+AGG | - | chr5.4:7383712-7383731 | None:intergenic | 40.0% | |
| TCAAGTAGAATCCATTCTGG+AGG | - | chr5.4:7384461-7384480 | None:intergenic | 40.0% | |
| TCTTGGAGAAAAGGAAGGAT+AGG | + | chr5.4:7384263-7384282 | MS.gene015369:CDS | 40.0% | |
| TTAAAGTAACACATGGGTGG+AGG | - | chr5.4:7384825-7384844 | None:intergenic | 40.0% | |
| TTGATTGAGAGAGATCTTGC+TGG | - | chr5.4:7384293-7384312 | None:intergenic | 40.0% | |
| ! | ATCATGGACTTTTCCACCAA+AGG | + | chr5.4:7383985-7384004 | MS.gene015369:CDS | 40.0% |
| !! | ATGTCTTTGGCTTTATCAGC+TGG | - | chr5.4:7383967-7383986 | None:intergenic | 40.0% |
| !! | TTCATCGGTTCTTGGAGAAA+AGG | + | chr5.4:7384254-7384273 | MS.gene015369:CDS | 40.0% |
| !! | TTCCTATTTTGGGAAACTCC+AGG | + | chr5.4:7383707-7383726 | MS.gene015369:CDS | 40.0% |
| AGTGAAGCTTTCCTCGTCAT+TGG | - | chr5.4:7384235-7384254 | None:intergenic | 45.0% | |
| ATCCATGACATGGCTCTCTT+TGG | + | chr5.4:7384760-7384779 | MS.gene015369:CDS | 45.0% | |
| CGAGGAAAGCTTCACTTCAT+CGG | + | chr5.4:7384239-7384258 | MS.gene015369:CDS | 45.0% | |
| TCGGTTCTTGGAGAAAAGGA+AGG | + | chr5.4:7384258-7384277 | MS.gene015369:CDS | 45.0% | |
| !! | AGCTTCACTTCATCGGTTCT+TGG | + | chr5.4:7384246-7384265 | MS.gene015369:CDS | 45.0% |
| GCTTGTTTGATATGGTGCCC+TGG | - | chr5.4:7384708-7384727 | None:intergenic | 50.0% | |
| TGCCAAAGAGAGCCATGTCA+TGG | - | chr5.4:7384765-7384784 | None:intergenic | 50.0% | |
| CGTAGCCAGAATTGCAGCCA+GGG | + | chr5.4:7384688-7384707 | MS.gene015369:intron | 55.0% | |
| TCGTAGCCAGAATTGCAGCC+AGG | + | chr5.4:7384687-7384706 | MS.gene015369:intron | 55.0% | |
| TGGTGCCCTGGCTGCAATTC+TGG | - | chr5.4:7384696-7384715 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 7383643 | 7384844 | 7383643 | ID=MS.gene015369 |
| chr5.4 | mRNA | 7383643 | 7384844 | 7383643 | ID=MS.gene015369.t1;Parent=MS.gene015369 |
| chr5.4 | exon | 7383643 | 7383728 | 7383643 | ID=MS.gene015369.t1.exon1;Parent=MS.gene015369.t1 |
| chr5.4 | CDS | 7383643 | 7383728 | 7383643 | ID=cds.MS.gene015369.t1;Parent=MS.gene015369.t1 |
| chr5.4 | exon | 7383873 | 7384117 | 7383873 | ID=MS.gene015369.t1.exon2;Parent=MS.gene015369.t1 |
| chr5.4 | CDS | 7383873 | 7384117 | 7383873 | ID=cds.MS.gene015369.t1;Parent=MS.gene015369.t1 |
| chr5.4 | exon | 7384227 | 7384284 | 7384227 | ID=MS.gene015369.t1.exon3;Parent=MS.gene015369.t1 |
| chr5.4 | CDS | 7384227 | 7384284 | 7384227 | ID=cds.MS.gene015369.t1;Parent=MS.gene015369.t1 |
| chr5.4 | exon | 7384697 | 7384844 | 7384697 | ID=MS.gene015369.t1.exon4;Parent=MS.gene015369.t1 |
| chr5.4 | CDS | 7384697 | 7384844 | 7384697 | ID=cds.MS.gene015369.t1;Parent=MS.gene015369.t1 |
| Gene Sequence |
| Protein sequence |
