Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015370.t1 | XP_024639117.1 | 83 | 188 | 24 | 3 | 1 | 184 | 1 | 184 | 4.00E-76 | 294.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015370.t1 | Q84R94 | 39.4 | 155 | 73 | 3 | 30 | 168 | 65 | 214 | 4.5e-20 | 99.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015370.t1 | A0A396HMK7 | 75.8 | 182 | 34 | 5 | 1 | 181 | 1 | 173 | 3.0e-65 | 257.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene015370.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015370.t1 | MTR_5g013515 | 91.241 | 137 | 11 | 1 | 33 | 168 | 1 | 137 | 9.68e-91 | 261 |
| MS.gene015370.t1 | MTR_5g013530 | 72.581 | 186 | 35 | 6 | 1 | 184 | 1 | 172 | 1.47e-86 | 252 |
| MS.gene015370.t1 | MTR_5g013520 | 76.974 | 152 | 27 | 3 | 32 | 182 | 1 | 145 | 1.53e-80 | 236 |
| MS.gene015370.t1 | MTR_5g013530 | 73.718 | 156 | 27 | 4 | 31 | 184 | 7 | 150 | 6.61e-74 | 219 |
| MS.gene015370.t1 | MTR_2g042900 | 46.012 | 163 | 46 | 6 | 27 | 163 | 99 | 245 | 2.56e-36 | 127 |
| MS.gene015370.t1 | MTR_2g042900 | 35.928 | 167 | 42 | 5 | 27 | 137 | 99 | 256 | 3.70e-22 | 90.1 |
| MS.gene015370.t1 | MTR_4g124960 | 35.878 | 131 | 60 | 3 | 40 | 169 | 95 | 202 | 2.60e-19 | 81.6 |
| MS.gene015370.t1 | MTR_4g124960 | 37.864 | 103 | 41 | 2 | 40 | 142 | 95 | 174 | 1.02e-16 | 74.3 |
| MS.gene015370.t1 | MTR_8g021380 | 29.221 | 154 | 91 | 3 | 29 | 176 | 88 | 229 | 5.69e-14 | 68.2 |
| MS.gene015370.t1 | MTR_4g124360 | 34.513 | 113 | 64 | 2 | 42 | 144 | 3 | 115 | 1.66e-13 | 64.7 |
| MS.gene015370.t1 | MTR_4g124360 | 34.513 | 113 | 64 | 2 | 42 | 144 | 41 | 153 | 2.86e-13 | 65.1 |
| MS.gene015370.t1 | MTR_2g044910 | 31.356 | 118 | 69 | 3 | 46 | 152 | 15 | 131 | 6.71e-13 | 63.9 |
| MS.gene015370.t1 | MTR_2g044910 | 29.054 | 148 | 84 | 4 | 25 | 152 | 28 | 174 | 1.09e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015370.t1 | AT1G19180 | 44.118 | 136 | 64 | 5 | 39 | 166 | 53 | 184 | 1.11e-26 | 100 |
| MS.gene015370.t1 | AT1G19180 | 44.118 | 136 | 64 | 5 | 39 | 166 | 119 | 250 | 4.67e-26 | 100 |
| MS.gene015370.t1 | AT1G19180 | 44.118 | 136 | 64 | 5 | 39 | 166 | 119 | 250 | 4.67e-26 | 100 |
| MS.gene015370.t1 | AT1G74950 | 39.130 | 138 | 70 | 4 | 39 | 164 | 112 | 247 | 1.75e-23 | 93.6 |
| MS.gene015370.t1 | AT1G74950 | 39.091 | 110 | 56 | 2 | 39 | 137 | 112 | 221 | 8.39e-18 | 78.2 |
| MS.gene015370.t1 | AT5G20900 | 36.719 | 128 | 69 | 5 | 32 | 148 | 41 | 167 | 8.30e-14 | 66.6 |
| MS.gene015370.t1 | AT1G72450 | 38.393 | 112 | 54 | 3 | 45 | 146 | 105 | 211 | 2.03e-13 | 67.0 |
| MS.gene015370.t1 | AT1G17380 | 30.000 | 150 | 86 | 3 | 11 | 146 | 63 | 207 | 2.13e-13 | 67.0 |
| MS.gene015370.t1 | AT1G17380 | 32.540 | 126 | 66 | 3 | 35 | 146 | 58 | 178 | 2.39e-13 | 66.6 |
| MS.gene015370.t1 | AT1G70700 | 35.577 | 104 | 62 | 3 | 46 | 145 | 119 | 221 | 1.38e-11 | 61.6 |
Find 36 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTACAAGAGGTTTCAAATTT+AGG | 0.093614 | 5.4:+7351784 | MS.gene015370:CDS |
| TCATCCTCACCTGTTATTTA+TGG | 0.150258 | 5.4:+7352139 | MS.gene015370:CDS |
| CATGACATGAATTCATTAAT+TGG | 0.189235 | 5.4:-7353410 | None:intergenic |
| ATTCATTAATTGGCTTATTA+AGG | 0.221545 | 5.4:-7353400 | None:intergenic |
| AGCTTCACTTCATCGGTTCT+TGG | 0.292747 | 5.4:+7352290 | MS.gene015370:CDS |
| GTTGGGTTACTTGTTTGATA+TGG | 0.313532 | 5.4:-7353374 | None:intergenic |
| TTTCAAATTTAGGAAATTCC+AGG | 0.358166 | 5.4:+7351794 | MS.gene015370:CDS |
| ATTGGCTTATTAAGGTTAGT+TGG | 0.360045 | 5.4:-7353392 | None:intergenic |
| TTGAGAGTTAGTAGTTCCTT+TGG | 0.375053 | 5.4:-7352029 | None:intergenic |
| TTGGCTTATTAAGGTTAGTT+GGG | 0.404409 | 5.4:-7353391 | None:intergenic |
| AGTGAAGCTTTCCTAGTCAT+TGG | 0.425048 | 5.4:-7352276 | None:intergenic |
| GAATTTCTAGCAAGAAATGA+TGG | 0.446507 | 5.4:-7352094 | None:intergenic |
| TGGAGCTACATTCTGATTTG+TGG | 0.458315 | 5.4:-7353463 | None:intergenic |
| ATTGTCATTTGTGCACATCT+AGG | 0.463250 | 5.4:-7351932 | None:intergenic |
| TTCATCGGTTCTTGGAGAAA+AGG | 0.463618 | 5.4:+7352298 | MS.gene015370:CDS |
| CATTCTGATTTGTGGTCTTG+TGG | 0.464643 | 5.4:-7353455 | None:intergenic |
| TGATACTTGAAGAGAATTGT+TGG | 0.494345 | 5.4:-7352119 | None:intergenic |
| AAGTTAAAGAAACACAGAAC+TGG | 0.498460 | 5.4:-7353483 | None:intergenic |
| AGAGTTAGTAGTTCCTTTGG+AGG | 0.509053 | 5.4:-7352026 | None:intergenic |
| TTGTGGTCTTGTGGTGATTG+TGG | 0.515403 | 5.4:-7353446 | None:intergenic |
| ATTCATGTCATGGCTCTCCT+TGG | 0.527756 | 5.4:+7353421 | MS.gene015370:CDS |
| CTAGGAAAGCTTCACTTCAT+CGG | 0.529148 | 5.4:+7352283 | MS.gene015370:CDS |
| GAAAATTCAACATAGGTGTA+CGG | 0.542214 | 5.4:-7351738 | None:intergenic |
| TGAAAGCATAGTTATTGTTG+TGG | 0.546555 | 5.4:-7352054 | None:intergenic |
| AATGAAGGCTAATATTAAGA+AGG | 0.546955 | 5.4:+7351904 | MS.gene015370:CDS |
| TCTTGGAGAAAAGGAAGGAT+AGG | 0.567398 | 5.4:+7352307 | MS.gene015370:CDS |
| ACATGTTGAAAATTCAACAT+AGG | 0.573945 | 5.4:-7351745 | None:intergenic |
| TTGTGGTGATTGTGGTGCCA+AGG | 0.574286 | 5.4:-7353438 | None:intergenic |
| TCCTCATCTCTCTCTACAAG+AGG | 0.582418 | 5.4:+7351771 | MS.gene015370:CDS |
| TCGGTTCTTGGAGAAAAGGA+AGG | 0.585705 | 5.4:+7352302 | MS.gene015370:CDS |
| CAATTAATGAATTCATGTCA+TGG | 0.607012 | 5.4:+7353411 | MS.gene015370:CDS |
| TTTACCATAAATAACAGGTG+AGG | 0.612964 | 5.4:-7352143 | None:intergenic |
| ATACAGATTTACCAATGACT+AGG | 0.627962 | 5.4:+7352265 | MS.gene015370:intron |
| AATTTCTAGCAAGAAATGAT+GGG | 0.637681 | 5.4:-7352093 | None:intergenic |
| ACCTCTTGTAGAGAGAGATG+AGG | 0.644344 | 5.4:-7351772 | None:intergenic |
| TTCTAGCAAGAAATGATGGG+TGG | 0.652275 | 5.4:-7352090 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGGTAAAAAAAATAATAAT+TGG | + | chr5.4:7352158-7352177 | MS.gene015370:intron | 10.0% |
| !!! | AAAAAAATCTAATTTTTTTC+GGG | + | chr5.4:7352687-7352706 | MS.gene015370:intron | 10.0% |
| !!! | AATATGAATTTTTATGTTTT+TGG | - | chr5.4:7352651-7352670 | None:intergenic | 10.0% |
| !! | ATTATCAAATAAAAGTTCAT+CGG | - | chr5.4:7352916-7352935 | None:intergenic | 15.0% |
| !! | CAAAAATATCAGTAAAAATA+TGG | - | chr5.4:7353323-7353342 | None:intergenic | 15.0% |
| !!! | AAATGACAATTTTTTATGAT+GGG | + | chr5.4:7351945-7351964 | MS.gene015370:CDS | 15.0% |
| !!! | AAGTTTTTCATCATTTATTT+TGG | + | chr5.4:7352479-7352498 | MS.gene015370:intron | 15.0% |
| !!! | AATTTGACAAAAATTTTAGA+AGG | - | chr5.4:7352448-7352467 | None:intergenic | 15.0% |
| !!! | ATCTTTTTTTTTTTTTTTGC+TGG | + | chr5.4:7352998-7353017 | MS.gene015370:intron | 15.0% |
| !!! | ATTAATTCCTATTTCATTTT+TGG | - | chr5.4:7352873-7352892 | None:intergenic | 15.0% |
| !!! | TATTTGATAATTGATAGATT+AGG | + | chr5.4:7352925-7352944 | MS.gene015370:intron | 15.0% |
| !!! | TTAATTCCTATTTCATTTTT+GGG | - | chr5.4:7352872-7352891 | None:intergenic | 15.0% |
| !!! | TTTTTTTTACCATAAATAAC+AGG | - | chr5.4:7352151-7352170 | None:intergenic | 15.0% |
| !! | CAAAAATCGTAATAGAAAAA+AGG | - | chr5.4:7353296-7353315 | None:intergenic | 20.0% |
| !! | GAGTTGAATAAAAGTAATTA+AGG | - | chr5.4:7352506-7352525 | None:intergenic | 20.0% |
| !!! | AATGACATTTTTTCAGAAAT+AGG | - | chr5.4:7352734-7352753 | None:intergenic | 20.0% |
| !!! | ATTCATTAATTGGCTTATTA+AGG | - | chr5.4:7353403-7353422 | None:intergenic | 20.0% |
| !!! | CAAATGACAATTTTTTATGA+TGG | + | chr5.4:7351944-7351963 | MS.gene015370:CDS | 20.0% |
| !!! | TTATTTTTACAGTGTAATGA+AGG | + | chr5.4:7351889-7351908 | MS.gene015370:intron | 20.0% |
| !!! | TTTTGGGCTTAATATATTTT+TGG | - | chr5.4:7352856-7352875 | None:intergenic | 20.0% |
| ! | AAAAAGGTTATCAATTTGAC+AGG | - | chr5.4:7353280-7353299 | None:intergenic | 25.0% |
| ! | AATGAAGGCTAATATTAAGA+AGG | + | chr5.4:7351904-7351923 | MS.gene015370:CDS | 25.0% |
| ! | ACATGTTGAAAATTCAACAT+AGG | - | chr5.4:7351748-7351767 | None:intergenic | 25.0% |
| ! | ATTAAGCCCAAAAATGAAAT+AGG | + | chr5.4:7352863-7352882 | MS.gene015370:intron | 25.0% |
| ! | ATTATCTGACTTTCAATCAA+TGG | - | chr5.4:7353165-7353184 | None:intergenic | 25.0% |
| ! | ATTGAAATCAACAATTCCTT+TGG | + | chr5.4:7353183-7353202 | MS.gene015370:intron | 25.0% |
| ! | CAATTAATGAATTCATGTCA+TGG | + | chr5.4:7353411-7353430 | MS.gene015370:CDS | 25.0% |
| ! | CATGACATGAATTCATTAAT+TGG | - | chr5.4:7353413-7353432 | None:intergenic | 25.0% |
| ! | CCTACTAAAAAAAATGTTGA+AGG | - | chr5.4:7351854-7351873 | None:intergenic | 25.0% |
| ! | TAATTGGATATCTATTTGAG+TGG | + | chr5.4:7352174-7352193 | MS.gene015370:intron | 25.0% |
| ! | TACAATCAAACAGTAATTAG+TGG | - | chr5.4:7352374-7352393 | None:intergenic | 25.0% |
| ! | TTTCAAATTTAGGAAATTCC+AGG | + | chr5.4:7351794-7351813 | MS.gene015370:CDS | 25.0% |
| !! | AGATTCAATACTTTTGCAAA+AGG | - | chr5.4:7353040-7353059 | None:intergenic | 25.0% |
| !! | GTTAATTCTGAAAACTTAAG+AGG | + | chr5.4:7352952-7352971 | MS.gene015370:intron | 25.0% |
| !! | TATGAGTTGACTAAAAAGTA+AGG | + | chr5.4:7352773-7352792 | MS.gene015370:intron | 25.0% |
| !! | TCTTATTTGTTTGAAAGATC+TGG | - | chr5.4:7352341-7352360 | None:intergenic | 25.0% |
| !!! | AATTTCTAGCAAGAAATGAT+GGG | - | chr5.4:7352096-7352115 | None:intergenic | 25.0% |
| !!! | CCTTCAACATTTTTTTTAGT+AGG | + | chr5.4:7351851-7351870 | MS.gene015370:intron | 25.0% |
| !!! | CTTGATTTTGATTTGTGTAA+TGG | + | chr5.4:7352202-7352221 | MS.gene015370:intron | 25.0% |
| ATACAGATTTACCAATGACT+AGG | + | chr5.4:7352265-7352284 | MS.gene015370:intron | 30.0% | |
| ATTTCGGAACAAGAAGTAAA+GGG | - | chr5.4:7353248-7353267 | None:intergenic | 30.0% | |
| CTACAAGAGGTTTCAAATTT+AGG | + | chr5.4:7351784-7351803 | MS.gene015370:CDS | 30.0% | |
| GAAAATTCAACATAGGTGTA+CGG | - | chr5.4:7351741-7351760 | None:intergenic | 30.0% | |
| TATTTCGGAACAAGAAGTAA+AGG | - | chr5.4:7353249-7353268 | None:intergenic | 30.0% | |
| TGAAAAAGTCAAGGACATTA+TGG | + | chr5.4:7351997-7352016 | MS.gene015370:CDS | 30.0% | |
| TGATACTTGAAGAGAATTGT+TGG | - | chr5.4:7352122-7352141 | None:intergenic | 30.0% | |
| TTTACCATAAATAACAGGTG+AGG | - | chr5.4:7352146-7352165 | None:intergenic | 30.0% | |
| ! | ATTGGCTTATTAAGGTTAGT+TGG | - | chr5.4:7353395-7353414 | None:intergenic | 30.0% |
| ! | TGAAAGCATAGTTATTGTTG+TGG | - | chr5.4:7352057-7352076 | None:intergenic | 30.0% |
| ! | TTCAATACTTTTGCAAAAGG+AGG | - | chr5.4:7353037-7353056 | None:intergenic | 30.0% |
| ! | TTGGCTTATTAAGGTTAGTT+GGG | - | chr5.4:7353394-7353413 | None:intergenic | 30.0% |
| !! | GAATTTCTAGCAAGAAATGA+TGG | - | chr5.4:7352097-7352116 | None:intergenic | 30.0% |
| ATTTGTTTGAAAGATCTGGC+TGG | - | chr5.4:7352337-7352356 | None:intergenic | 35.0% | |
| GTTGGGTTACTTGTTTGATA+TGG | - | chr5.4:7353377-7353396 | None:intergenic | 35.0% | |
| TATTCCAGCTGAAAAAGTCA+AGG | + | chr5.4:7351988-7352007 | MS.gene015370:CDS | 35.0% | |
| TCAACAATTCCTTTGGACAT+AGG | + | chr5.4:7353190-7353209 | MS.gene015370:intron | 35.0% | |
| TGACAGGACTCAAACTATTT+CGG | - | chr5.4:7353264-7353283 | None:intergenic | 35.0% | |
| TTGAGAGTTAGTAGTTCCTT+TGG | - | chr5.4:7352032-7352051 | None:intergenic | 35.0% | |
| ! | ATTATGGTCTTTTCCTCCAA+AGG | + | chr5.4:7352013-7352032 | MS.gene015370:CDS | 35.0% |
| ! | ATTGTCATTTGTGCACATCT+AGG | - | chr5.4:7351935-7351954 | None:intergenic | 35.0% |
| ! | TCATCCTCACCTGTTATTTA+TGG | + | chr5.4:7352139-7352158 | MS.gene015370:CDS | 35.0% |
| AGAGTTAGTAGTTCCTTTGG+AGG | - | chr5.4:7352029-7352048 | None:intergenic | 40.0% | |
| AGTGAAGCTTTCCTAGTCAT+TGG | - | chr5.4:7352279-7352298 | None:intergenic | 40.0% | |
| CATGCATTTCCTATGTCCAA+AGG | - | chr5.4:7353202-7353221 | None:intergenic | 40.0% | |
| CTAGGAAAGCTTCACTTCAT+CGG | + | chr5.4:7352283-7352302 | MS.gene015370:CDS | 40.0% | |
| GCCATCAATTTGTGATCAGT+CGG | + | chr5.4:7353062-7353081 | MS.gene015370:intron | 40.0% | |
| GTAGCAAAAGAACATGTACC+TGG | - | chr5.4:7351815-7351834 | None:intergenic | 40.0% | |
| TCTTGGAGAAAAGGAAGGAT+AGG | + | chr5.4:7352307-7352326 | MS.gene015370:CDS | 40.0% | |
| ! | CATTCTGATTTGTGGTCTTG+TGG | - | chr5.4:7353458-7353477 | None:intergenic | 40.0% |
| ! | TGGAGCTACATTCTGATTTG+TGG | - | chr5.4:7353466-7353485 | None:intergenic | 40.0% |
| ! | TTCTAGCAAGAAATGATGGG+TGG | - | chr5.4:7352093-7352112 | None:intergenic | 40.0% |
| !! | TTCATCGGTTCTTGGAGAAA+AGG | + | chr5.4:7352298-7352317 | MS.gene015370:CDS | 40.0% |
| !!! | ATGTCCTTGACTTTTTCAGC+TGG | - | chr5.4:7351995-7352014 | None:intergenic | 40.0% |
| ACCTCTTGTAGAGAGAGATG+AGG | - | chr5.4:7351775-7351794 | None:intergenic | 45.0% | |
| ATTCATGTCATGGCTCTCCT+TGG | + | chr5.4:7353421-7353440 | MS.gene015370:CDS | 45.0% | |
| GCCGACTGATCACAAATTGA+TGG | - | chr5.4:7353066-7353085 | None:intergenic | 45.0% | |
| TCCTCATCTCTCTCTACAAG+AGG | + | chr5.4:7351771-7351790 | MS.gene015370:CDS | 45.0% | |
| TCGGTTCTTGGAGAAAAGGA+AGG | + | chr5.4:7352302-7352321 | MS.gene015370:CDS | 45.0% | |
| !! | AGCTTCACTTCATCGGTTCT+TGG | + | chr5.4:7352290-7352309 | MS.gene015370:CDS | 45.0% |
| !! | TTGTGGTCTTGTGGTGATTG+TGG | - | chr5.4:7353449-7353468 | None:intergenic | 45.0% |
| !!! | TAAAAAAATCTAATTTTTTT+CGG | + | chr5.4:7352686-7352705 | MS.gene015370:intron | 5.0% |
| ! | TTGTGGTGATTGTGGTGCCA+AGG | - | chr5.4:7353441-7353460 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 7351724 | 7353502 | 7351724 | ID=MS.gene015370 |
| chr5.4 | mRNA | 7351724 | 7353502 | 7351724 | ID=MS.gene015370.t1;Parent=MS.gene015370 |
| chr5.4 | exon | 7351724 | 7351815 | 7351724 | ID=MS.gene015370.t1.exon1;Parent=MS.gene015370.t1 |
| chr5.4 | CDS | 7351724 | 7351815 | 7351724 | ID=cds.MS.gene015370.t1;Parent=MS.gene015370.t1 |
| chr5.4 | exon | 7351901 | 7352160 | 7351901 | ID=MS.gene015370.t1.exon2;Parent=MS.gene015370.t1 |
| chr5.4 | CDS | 7351901 | 7352160 | 7351901 | ID=cds.MS.gene015370.t1;Parent=MS.gene015370.t1 |
| chr5.4 | exon | 7352271 | 7352328 | 7352271 | ID=MS.gene015370.t1.exon3;Parent=MS.gene015370.t1 |
| chr5.4 | CDS | 7352271 | 7352328 | 7352271 | ID=cds.MS.gene015370.t1;Parent=MS.gene015370.t1 |
| chr5.4 | exon | 7353358 | 7353502 | 7353358 | ID=MS.gene015370.t1.exon4;Parent=MS.gene015370.t1 |
| chr5.4 | CDS | 7353358 | 7353502 | 7353358 | ID=cds.MS.gene015370.t1;Parent=MS.gene015370.t1 |
| Gene Sequence |
| Protein sequence |
