AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01559

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01559.t1 MTR_2g016030 88.820 161 17 1 1 160 1 161 3.81e-103 292
MS.gene01559.t1 MTR_6g012450 69.136 81 24 1 19 99 110 189 2.85e-36 130
MS.gene01559.t1 MTR_4g082060 64.948 97 29 3 33 127 110 203 3.29e-35 127
MS.gene01559.t1 MTR_8g044220 62.791 86 31 1 25 109 96 181 4.41e-35 127
MS.gene01559.t1 MTR_7g010950 69.737 76 22 1 24 99 114 188 1.22e-34 126
MS.gene01559.t1 MTR_7g086780 69.863 73 21 1 28 99 80 152 1.80e-32 119
MS.gene01559.t1 MTR_5g041380 56.989 93 38 1 18 108 97 189 9.34e-32 117
MS.gene01559.t1 MTR_6g027460 70.423 71 20 1 30 99 79 149 4.95e-31 115
MS.gene01559.t1 MTR_6g027450 69.014 71 21 1 30 99 72 142 1.50e-30 112
MS.gene01559.t1 MTR_3g435480 62.667 75 27 1 25 99 116 189 3.32e-30 114
MS.gene01559.t1 MTR_5g041530 69.014 71 19 1 32 99 123 193 4.19e-30 112
MS.gene01559.t1 MTR_5g041400 61.039 77 30 0 32 108 105 181 1.13e-29 111
MS.gene01559.t1 MTR_5g041420 55.422 83 32 1 17 99 114 191 1.21e-29 110
MS.gene01559.t1 MTR_2g096740 57.895 76 32 0 24 99 40 115 2.37e-28 106
MS.gene01559.t1 MTR_2g096740 57.895 76 32 0 24 99 55 130 2.69e-28 106
MS.gene01559.t1 MTR_4g022370 71.930 57 16 0 43 99 79 135 4.79e-26 101
MS.gene01559.t1 MTR_8g015840 57.746 71 24 1 34 98 2 72 1.19e-25 97.8
MS.gene01559.t1 MTR_8g068210 45.545 101 52 2 28 127 13 111 1.50e-25 99.8
MS.gene01559.t1 MTR_8g479350 47.000 100 42 2 48 138 70 167 1.78e-25 99.8
MS.gene01559.t1 MTR_8g079060 69.231 52 16 0 46 97 29 80 9.85e-25 95.5
MS.gene01559.t1 MTR_8g027295 54.878 82 33 2 36 113 12 93 1.00e-24 96.7
MS.gene01559.t1 MTR_4g063780 72.727 55 15 0 43 97 72 126 1.23e-24 97.4
MS.gene01559.t1 MTR_7g024670 78.000 50 11 0 48 97 79 128 1.53e-24 97.8
MS.gene01559.t1 MTR_4g109980 69.811 53 16 0 44 96 38 90 1.99e-24 96.7
MS.gene01559.t1 MTR_2g059540 73.469 49 13 0 46 94 31 79 3.26e-24 93.6
MS.gene01559.t1 MTR_7g059400 70.175 57 17 0 43 99 73 129 3.64e-24 96.3
MS.gene01559.t1 MTR_2g093220 57.971 69 29 0 31 99 25 93 4.73e-24 95.1
MS.gene01559.t1 MTR_3g091820 63.158 57 21 0 43 99 40 96 5.48e-24 95.1
MS.gene01559.t1 MTR_4g088580 54.878 82 28 2 48 120 57 138 1.01e-23 95.5
MS.gene01559.t1 MTR_5g031440 68.519 54 17 0 43 96 40 93 2.18e-23 94.0
MS.gene01559.t1 MTR_1g077600 61.818 55 21 0 43 97 40 94 3.43e-23 92.4
MS.gene01559.t1 MTR_1g077600 61.818 55 21 0 43 97 40 94 3.82e-23 92.8
MS.gene01559.t1 MTR_4g089095 67.308 52 17 0 48 99 52 103 4.20e-23 92.8
MS.gene01559.t1 MTR_4g089095 67.308 52 17 0 48 99 18 69 4.85e-23 92.0
MS.gene01559.t1 MTR_2g014170 71.429 49 14 0 46 94 45 93 1.71e-22 91.3
MS.gene01559.t1 MTR_4g461080 52.703 74 35 0 24 97 7 80 2.35e-22 90.5
MS.gene01559.t1 MTR_2g013370 62.963 54 20 0 46 99 46 99 2.60e-22 90.1
MS.gene01559.t1 MTR_1g056810 62.903 62 22 1 39 99 4 65 3.33e-22 90.1
MS.gene01559.t1 MTR_3g090430 63.793 58 21 0 42 99 47 104 3.63e-22 90.9
MS.gene01559.t1 MTR_2g014060 48.052 77 40 0 22 98 1 77 4.63e-22 90.5
MS.gene01559.t1 MTR_3g077750 66.667 51 17 0 46 96 61 111 1.52e-21 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01559.t1 AT1G29160 61.404 114 24 1 8 101 4 117 1.26e-45 147
MS.gene01559.t1 AT2G34140 61.818 110 26 1 8 101 4 113 5.36e-44 143
MS.gene01559.t1 AT3G47500 58.252 103 43 0 20 122 84 186 2.54e-35 127
MS.gene01559.t1 AT5G62430 59.000 100 29 2 12 99 8 107 7.92e-35 123
MS.gene01559.t1 AT5G39660 80.952 63 12 0 40 102 132 194 3.95e-34 124
MS.gene01559.t1 AT5G39660 80.952 63 12 0 40 102 132 194 3.95e-34 124
MS.gene01559.t1 AT5G39660 80.952 63 12 0 40 102 132 194 3.95e-34 124
MS.gene01559.t1 AT1G26790 55.340 103 39 1 23 125 88 183 1.00e-33 122
MS.gene01559.t1 AT1G69570 59.341 91 30 1 34 124 120 203 2.46e-32 119
MS.gene01559.t1 AT2G28810 74.545 55 14 0 43 97 74 128 6.06e-26 100
MS.gene01559.t1 AT5G60850 72.222 54 15 0 46 99 51 104 7.80e-26 100
MS.gene01559.t1 AT2G28810 74.545 55 14 0 43 97 91 145 9.92e-26 100
MS.gene01559.t1 AT1G51700 69.811 53 16 0 46 98 33 85 1.11e-25 97.4
MS.gene01559.t1 AT3G21270 75.000 52 13 0 46 97 29 80 2.19e-25 96.7
MS.gene01559.t1 AT2G37590 70.175 57 17 0 43 99 86 142 3.06e-25 99.0
MS.gene01559.t1 AT3G55370 70.175 57 17 0 43 99 73 129 3.11e-25 99.0
MS.gene01559.t1 AT3G55370 70.175 57 17 0 43 99 73 129 3.28e-25 99.4
MS.gene01559.t1 AT3G55370 70.175 57 17 0 43 99 118 174 4.47e-25 99.0
MS.gene01559.t1 AT3G50410 69.811 53 16 0 46 98 30 82 5.21e-25 97.1
MS.gene01559.t1 AT4G38000 58.824 68 27 1 43 109 38 105 5.92e-25 96.7
MS.gene01559.t1 AT1G07640 53.750 80 37 0 18 97 49 128 7.51e-25 97.8
MS.gene01559.t1 AT1G07640 53.750 80 37 0 18 97 57 136 8.53e-25 97.8
MS.gene01559.t1 AT1G07640 78.000 50 11 0 48 97 23 72 1.10e-24 96.7
MS.gene01559.t1 AT5G65590 66.667 57 19 0 43 99 38 94 3.34e-24 95.9
MS.gene01559.t1 AT5G02460 70.175 57 17 0 43 99 92 148 5.92e-24 96.3
MS.gene01559.t1 AT5G66940 53.425 73 34 0 46 118 32 104 1.54e-23 92.4
MS.gene01559.t1 AT3G45610 52.778 72 34 0 28 99 22 93 1.97e-23 92.8
MS.gene01559.t1 AT5G60200 64.912 57 20 0 43 99 52 108 2.92e-23 92.4
MS.gene01559.t1 AT1G47655 64.815 54 19 0 41 94 24 77 3.66e-23 91.3
MS.gene01559.t1 AT2G28510 73.077 52 14 0 48 99 50 101 3.86e-23 92.8
MS.gene01559.t1 AT4G24060 61.404 57 22 0 43 99 50 106 5.53e-23 93.2
MS.gene01559.t1 AT1G21340 50.000 94 38 2 48 138 40 127 8.19e-23 91.3
MS.gene01559.t1 AT1G28310 66.667 54 18 0 46 99 27 80 9.28e-23 92.0
MS.gene01559.t1 AT3G52440 64.407 59 20 1 44 101 41 99 9.87e-23 91.3
MS.gene01559.t1 AT3G52440 46.000 100 48 3 44 137 22 121 1.01e-22 90.9
MS.gene01559.t1 AT3G61850 56.250 64 28 0 36 99 52 115 1.12e-22 91.3
MS.gene01559.t1 AT3G61850 56.250 64 28 0 36 99 52 115 1.12e-22 91.3
MS.gene01559.t1 AT1G64620 65.385 52 18 0 43 94 46 97 1.24e-22 92.4
MS.gene01559.t1 AT3G61850 56.250 64 28 0 36 99 64 127 1.25e-22 91.7
MS.gene01559.t1 AT1G28310 66.667 54 18 0 46 99 51 104 1.34e-22 92.0
MS.gene01559.t1 AT3G61850 56.250 64 28 0 36 99 52 115 1.86e-22 91.3
MS.gene01559.t1 AT3G61850 56.250 64 28 0 36 99 64 127 1.90e-22 91.3
MS.gene01559.t1 AT4G00940 69.231 52 16 0 48 99 70 121 4.05e-22 90.1
MS.gene01559.t1 AT4G00940 69.231 52 16 0 48 99 70 121 4.05e-22 90.1
MS.gene01559.t1 AT4G00940 69.231 52 16 0 48 99 70 121 4.05e-22 90.1
MS.gene01559.t1 AT5G62940 69.388 49 15 0 48 96 75 123 1.19e-21 89.7
MS.gene01559.t1 AT4G21050 57.143 63 27 0 48 110 26 88 5.06e-21 85.5
MS.gene01559.t1 AT2G46590 68.750 48 15 0 46 93 68 115 6.14e-21 87.8
MS.gene01559.t1 AT2G46590 68.750 48 15 0 46 93 80 127 7.08e-21 87.8
MS.gene01559.t1 AT4G21040 61.667 60 19 1 48 103 27 86 8.63e-20 82.8
MS.gene01559.t1 AT4G21080 55.882 68 26 1 48 111 27 94 3.55e-19 81.6
MS.gene01559.t1 AT4G21030 44.118 68 38 0 48 115 23 90 4.85e-14 67.0

Find 46 sgRNAs with CRISPR-Local

Find 50 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACAAGGGATTAAGCTATT+TGG 0.296201 2.2:+67528044 MS.gene01559:CDS
GGTCTTCCTCCGCCGCTTGC+CGG 0.325640 2.2:-67528444 None:intergenic
GCTTCATAGACACAACTTTC+CGG 0.327262 2.2:-67528341 None:intergenic
GTTCTAACCCTAAGTAGTTC+TGG 0.342013 2.2:-67528382 None:intergenic
GTAAGAGCTGTCAAAGATAC+TGG 0.350596 2.2:+67528234 MS.gene01559:CDS
ATGTCCAAGATGCAAGAGTA+TGG 0.357770 2.2:+67528157 MS.gene01559:CDS
GATGATGGCCAGAACTACTT+AGG 0.413991 2.2:+67528374 MS.gene01559:CDS
GTTTCCATACTCTTGCATCT+TGG 0.414049 2.2:-67528161 None:intergenic
CTCCGACGACCTGCTCCAAC+CGG 0.429962 2.2:-67528284 None:intergenic
TGGGGCCCTCCGTAACGTTC+CGG 0.432988 2.2:+67528265 MS.gene01559:CDS
ATCCTCAAGACTTGGCGGCT+TGG 0.460966 2.2:-67528309 None:intergenic
CGTAACGTTCCGGTTGGAGC+AGG 0.491556 2.2:+67528275 MS.gene01559:CDS
CAAAGATACTGGACGGCTGG+TGG 0.496439 2.2:+67528245 MS.gene01559:CDS
AAAAGAAAGTGAAGATCAAA+CGG 0.511866 2.2:+67528106 MS.gene01559:CDS
CTGCTCCAACCGGAACGTTA+CGG 0.518351 2.2:-67528274 None:intergenic
GCCCTCCGTAACGTTCCGGT+TGG 0.526190 2.2:+67528269 MS.gene01559:CDS
AAAGATACTGGACGGCTGGT+GGG 0.536772 2.2:+67528246 MS.gene01559:CDS
TTGCATGAGGAATTAAAAGA+AGG 0.537402 2.2:+67528080 MS.gene01559:CDS
TCTTGAGGATAGCGGTTCAC+CGG 0.541063 2.2:+67528322 MS.gene01559:CDS
ATGATGGCCAGAACTACTTA+GGG 0.541176 2.2:+67528375 MS.gene01559:CDS
TTCCGGTTGGAGCAGGTCGT+CGG 0.541484 2.2:+67528282 MS.gene01559:CDS
TGTCAAAGATACTGGACGGC+TGG 0.542444 2.2:+67528242 MS.gene01559:CDS
CGGTTGGAGCAGGTCGTCGG+AGG 0.543759 2.2:+67528285 MS.gene01559:CDS
AGAAAAGGTGAAGAGATACA+AGG 0.544735 2.2:+67528029 MS.gene01559:CDS
ATACTCTTGCATCTTGGACA+TGG 0.546078 2.2:-67528155 None:intergenic
GGCCAAGCCGCCAAGTCTTG+AGG 0.555303 2.2:+67528307 MS.gene01559:CDS
AAGCGGCGGAGGAAGACCTC+AGG 0.572311 2.2:+67528449 MS.gene01559:CDS
GAAGCTGCTAATTCTGATGA+TGG 0.575725 2.2:+67528359 MS.gene01559:CDS
AAAATGGCTGAAGTGAGAAA+AGG 0.579551 2.2:+67528014 None:intergenic
AAGATACTGGACGGCTGGTG+GGG 0.581078 2.2:+67528247 MS.gene01559:CDS
CCGCTATCCTCAAGACTTGG+CGG 0.597183 2.2:-67528314 None:intergenic
GAACCGCTATCCTCAAGACT+TGG 0.599554 2.2:-67528317 None:intergenic
GAAAAGGTGAAGAGATACAA+GGG 0.603099 2.2:+67528030 MS.gene01559:CDS
GGTTGGAGCAGGTCGTCGGA+GGG 0.610139 2.2:+67528286 MS.gene01559:CDS
CACAACAATAAAATTGCATG+AGG 0.620119 2.2:+67528067 MS.gene01559:CDS
CGGCGGAGGAAGACCTCAGG+TGG 0.630380 2.2:+67528452 MS.gene01559:CDS
TGCTAATGAATAACCACCTG+AGG 0.631649 2.2:-67528465 None:intergenic
GAGCTGTCAAAGATACTGGA+CGG 0.644495 2.2:+67528238 MS.gene01559:CDS
TCCAACCGGAACGTTACGGA+GGG 0.647718 2.2:-67528270 None:intergenic
TCCCGAAAATCACTCTGAGG+AGG 0.648141 2.2:-67528413 None:intergenic
CTCCAACCGGAACGTTACGG+AGG 0.649811 2.2:-67528271 None:intergenic
ACTTCCCGAAAATCACTCTG+AGG 0.686816 2.2:-67528416 None:intergenic
GTAAAGAACAAGAAATCACA+AGG 0.691544 2.2:-67528492 None:intergenic
AGAAAGTGAAGATCAAACGG+TGG 0.696077 2.2:+67528109 MS.gene01559:CDS
CCGCCAAGTCTTGAGGATAG+CGG 0.704408 2.2:+67528314 MS.gene01559:CDS
AAGAACAAGAAATCACAAGG+AGG 0.761754 2.2:-67528489 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAAGTGAAGATCAAA+CGG + chr2.2:67528106-67528125 MS.gene01559:CDS 25.0%
ATACAAGGGATTAAGCTATT+TGG + chr2.2:67528044-67528063 MS.gene01559:CDS 30.0%
CACAACAATAAAATTGCATG+AGG + chr2.2:67528067-67528086 MS.gene01559:CDS 30.0%
TTGCATGAGGAATTAAAAGA+AGG + chr2.2:67528080-67528099 MS.gene01559:CDS 30.0%
AGAAAAGGTGAAGAGATACA+AGG + chr2.2:67528029-67528048 MS.gene01559:CDS 35.0%
CAGCTCTTACAAAAATGTCT+TGG - chr2.2:67528224-67528243 None:intergenic 35.0%
GAAAAGGTGAAGAGATACAA+GGG + chr2.2:67528030-67528049 MS.gene01559:CDS 35.0%
!!! GATTTTCGGGAAGTATTTTC+CGG + chr2.2:67528425-67528444 MS.gene01559:CDS 35.0%
AGAAAGTGAAGATCAAACGG+TGG + chr2.2:67528109-67528128 MS.gene01559:CDS 40.0%
ATACTCTTGCATCTTGGACA+TGG - chr2.2:67528158-67528177 None:intergenic 40.0%
ATGATGGCCAGAACTACTTA+GGG + chr2.2:67528375-67528394 MS.gene01559:CDS 40.0%
ATGTCCAAGATGCAAGAGTA+TGG + chr2.2:67528157-67528176 MS.gene01559:CDS 40.0%
GAAGCTGCTAATTCTGATGA+TGG + chr2.2:67528359-67528378 MS.gene01559:CDS 40.0%
GCTTCATAGACACAACTTTC+CGG - chr2.2:67528344-67528363 None:intergenic 40.0%
GTAAGAGCTGTCAAAGATAC+TGG + chr2.2:67528234-67528253 MS.gene01559:CDS 40.0%
GTTCTAACCCTAAGTAGTTC+TGG - chr2.2:67528385-67528404 None:intergenic 40.0%
GTTTCCATACTCTTGCATCT+TGG - chr2.2:67528164-67528183 None:intergenic 40.0%
TGCTAATGAATAACCACCTG+AGG - chr2.2:67528468-67528487 None:intergenic 40.0%
ACTTCCCGAAAATCACTCTG+AGG - chr2.2:67528419-67528438 None:intergenic 45.0%
GAGCTGTCAAAGATACTGGA+CGG + chr2.2:67528238-67528257 MS.gene01559:CDS 45.0%
! GATGATGGCCAGAACTACTT+AGG + chr2.2:67528374-67528393 MS.gene01559:CDS 45.0%
! TGCCTCCTCAGAGTGATTTT+CGG + chr2.2:67528411-67528430 MS.gene01559:CDS 45.0%
AAAGATACTGGACGGCTGGT+GGG + chr2.2:67528246-67528265 MS.gene01559:CDS 50.0%
GAACCGCTATCCTCAAGACT+TGG - chr2.2:67528320-67528339 None:intergenic 50.0%
TGTCAAAGATACTGGACGGC+TGG + chr2.2:67528242-67528261 MS.gene01559:CDS 50.0%
! AAGTATTTTCCGGCAAGCGG+CGG + chr2.2:67528435-67528454 MS.gene01559:CDS 50.0%
! GCCTCCTCAGAGTGATTTTC+GGG + chr2.2:67528412-67528431 MS.gene01559:CDS 50.0%
! TCCCGAAAATCACTCTGAGG+AGG - chr2.2:67528416-67528435 None:intergenic 50.0%
! TCTTGAGGATAGCGGTTCAC+CGG + chr2.2:67528322-67528341 MS.gene01559:CDS 50.0%
!!! GGGAAGTATTTTCCGGCAAG+CGG + chr2.2:67528432-67528451 MS.gene01559:CDS 50.0%
AAGATACTGGACGGCTGGTG+GGG + chr2.2:67528247-67528266 MS.gene01559:CDS 55.0%
ATCCTCAAGACTTGGCGGCT+TGG - chr2.2:67528312-67528331 None:intergenic 55.0%
CAAAGATACTGGACGGCTGG+TGG + chr2.2:67528245-67528264 MS.gene01559:CDS 55.0%
CCGCCAAGTCTTGAGGATAG+CGG + chr2.2:67528314-67528333 MS.gene01559:CDS 55.0%
CCGCTATCCTCAAGACTTGG+CGG - chr2.2:67528317-67528336 None:intergenic 55.0%
CTGCTCCAACCGGAACGTTA+CGG - chr2.2:67528277-67528296 None:intergenic 55.0%
TCCAACCGGAACGTTACGGA+GGG - chr2.2:67528273-67528292 None:intergenic 55.0%
CGTAACGTTCCGGTTGGAGC+AGG + chr2.2:67528275-67528294 MS.gene01559:CDS 60.0%
CTCCAACCGGAACGTTACGG+AGG - chr2.2:67528274-67528293 None:intergenic 60.0%
TTCCGGTTGGAGCAGGTCGT+CGG + chr2.2:67528282-67528301 MS.gene01559:CDS 60.0%
! TATTTTCCGGCAAGCGGCGG+AGG + chr2.2:67528438-67528457 MS.gene01559:CDS 60.0%
AAGCGGCGGAGGAAGACCTC+AGG + chr2.2:67528449-67528468 MS.gene01559:CDS 65.0%
CTCCGACGACCTGCTCCAAC+CGG - chr2.2:67528287-67528306 None:intergenic 65.0%
GCCCTCCGTAACGTTCCGGT+TGG + chr2.2:67528269-67528288 MS.gene01559:CDS 65.0%
GGCCAAGCCGCCAAGTCTTG+AGG + chr2.2:67528307-67528326 MS.gene01559:CDS 65.0%
GGTTGGAGCAGGTCGTCGGA+GGG + chr2.2:67528286-67528305 MS.gene01559:CDS 65.0%
TGGGGCCCTCCGTAACGTTC+CGG + chr2.2:67528265-67528284 MS.gene01559:CDS 65.0%
CGGCGGAGGAAGACCTCAGG+TGG + chr2.2:67528452-67528471 MS.gene01559:CDS 70.0%
CGGTTGGAGCAGGTCGTCGG+AGG + chr2.2:67528285-67528304 MS.gene01559:CDS 70.0%
GGTCTTCCTCCGCCGCTTGC+CGG - chr2.2:67528447-67528466 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 67528017 67528499 67528017 ID=MS.gene01559
chr2.2 mRNA 67528017 67528499 67528017 ID=MS.gene01559.t1;Parent=MS.gene01559
chr2.2 exon 67528017 67528499 67528017 ID=MS.gene01559.t1.exon1;Parent=MS.gene01559.t1
chr2.2 CDS 67528017 67528499 67528017 ID=cds.MS.gene01559.t1;Parent=MS.gene01559.t1
Gene Sequence

>MS.gene01559

ATGGCTGAAGTGAGAAAAGGTGAAGAGATACAAGGGATTAAGCTATTTGGCACAACAATAAAATTGCATGAGGAATTAAAAGAAGGTCAAAAAGAAAGTGAAGATCAAACGGTGGAGAAGAAGATAGAAAAGATCATACCATGTCCAAGATGCAAGAGTATGGAAACTAAATTTTGTTATTTCAATAATTATAACGTTAATCAACCAAGACATTTTTGTAAGAGCTGTCAAAGATACTGGACGGCTGGTGGGGCCCTCCGTAACGTTCCGGTTGGAGCAGGTCGTCGGAGGGCCAAGCCGCCAAGTCTTGAGGATAGCGGTTCACCGGAAAGTTGTGTCTATGAAGCTGCTAATTCTGATGATGGCCAGAACTACTTAGGGTTAGAACAATTTATGCCTCCTCAGAGTGATTTTCGGGAAGTATTTTCCGGCAAGCGGCGGAGGAAGACCTCAGGTGGTTATTCATTAGCATCCTCCTTGTGA
Protein sequence

>MS.gene01559.t1

MAEVRKGEEIQGIKLFGTTIKLHEELKEGQKESEDQTVEKKIEKIIPCPRCKSMETKFCYFNNYNVNQPRHFCKSCQRYWTAGGALRNVPVGAGRRRAKPPSLEDSGSPESCVYEAANSDDGQNYLGLEQFMPPQSDFREVFSGKRRRKTSGGYSLASSL