AlfalfaGEDB | MS.gene01562

M. sativa cultivar XinJiangDaYe / MS.gene01562

KEGG: -
GO: -
PFAM: PF13837.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01562.t1	KEH36594.1	74.7	312	11	1	1	244	1	312	1.70E-101	379

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01562.t1	Q9LJG8	49.6	125	58	3	20	142	81	202	5.2e-27	122.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01562.t1	A0A072V3I8	74.7	312	11	1	1	244	1	312	1.2e-101	379.0

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene01562.t1	TF	Trihelix

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01562.t1	MTR_2g016140	94.624	186	10	0	1	186	1	186	1.78e-132	377
MS.gene01562.t1	MTR_2g016140	96.875	64	2	0	182	245	250	313	1.23e-35	129
MS.gene01562.t1	MTR_2g016140	94.624	186	10	0	1	186	1	186	1.83e-132	376
MS.gene01562.t1	MTR_2g016140	98.413	63	1	0	182	244	250	312	1.47e-35	129
MS.gene01562.t1	MTR_4g079960	50.171	293	87	7	11	249	19	306	7.66e-78	238
MS.gene01562.t1	MTR_4g079960	50.519	289	84	7	11	245	19	302	1.14e-77	237
MS.gene01562.t1	MTR_4g079960	50.699	286	82	7	19	250	27	307	1.86e-77	237
MS.gene01562.t1	MTR_8g022290	40.794	277	109	6	19	241	64	339	4.38e-49	166
MS.gene01562.t1	MTR_2g092960	44.776	134	70	3	11	144	34	163	8.98e-29	114
MS.gene01562.t1	MTR_6g486270	28.384	229	139	7	23	236	130	348	4.31e-26	104

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene01562.t1	AT3G58630	42.384	302	99	7	3	237	2	295	4.02e-63	200
MS.gene01562.t1	AT3G58630	42.333	300	102	6	3	237	2	295	6.85e-63	199
MS.gene01562.t1	AT5G05550	43.388	242	110	6	19	248	20	246	6.10e-50	164
MS.gene01562.t1	AT5G05550	44.635	233	102	6	19	239	20	237	8.27e-50	164
MS.gene01562.t1	AT5G05550	44.635	233	102	6	19	239	20	237	1.40e-49	163
MS.gene01562.t1	AT3G11100	41.880	234	112	5	19	239	18	240	5.86e-47	156
MS.gene01562.t1	AT3G14180	50.000	126	58	3	19	142	80	202	2.56e-35	130
MS.gene01562.t1	AT3G14180	46.970	66	33	1	184	247	356	421	2.36e-12	66.6
MS.gene01562.t1	AT3G24490	30.317	221	139	4	23	236	90	302	1.44e-27	108
MS.gene01562.t1	AT1G54060	51.020	98	44	2	19	116	90	183	1.18e-21	92.8

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Find 64 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAATCGTGGTGAAAATC+CGG	0.442007	2.2:-67452205	None:intergenic
AACTTCTTAGCACTGAAATT+AGG	0.324747	2.2:-67452550	None:intergenic
AATTGCGGTACATTCCACCG+CGG	0.821763	2.2:+67452675	MS.gene01562:CDS
AATTTCCGATGAGGAATCAA+CGG	0.616061	2.2:-67452622	None:intergenic
ACAAAGGATGCAGTTTGCTA+AGG	0.543201	2.2:+67453029	MS.gene01562:CDS
ACAATCCTCGCGAACGGGGA+CGG	0.571999	2.2:-67452245	None:intergenic
ACAGAAACTGAGGCAGATGG+TGG	0.614677	2.2:+67452996	MS.gene01562:CDS
ACCAACAATCCTCGCGAACG+GGG	0.660501	2.2:-67452249	None:intergenic
ACCCCATGCATCGACGAGAG+TGG	0.606737	2.2:-67452287	None:intergenic
ACGCACCGTCCCCGTTCGCG+AGG	0.609985	2.2:+67452240	MS.gene01562:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACATCTAACCGTTCAAAAAA+AGG	-	chr2.2:67452520-67452539	None:intergenic	30.0%
AGTATAAGGTTGAAAAAGCT+AGG	+	chr2.2:67452458-67452477	MS.gene01562:CDS	30.0%
ATCAAATCACAAGCTAAAAG+GGG	-	chr2.2:67453189-67453208	None:intergenic	30.0%
GAAGTTATCAATTTCTTCAC+CGG	+	chr2.2:67452567-67452586	MS.gene01562:CDS	30.0%
GTATAAGGTTGAAAAAGCTA+GGG	+	chr2.2:67452459-67452478	MS.gene01562:CDS	30.0%
AAAGAATCGTGGTGAAAATC+CGG	-	chr2.2:67452208-67452227	None:intergenic	35.0%
AATTTCCGATGAGGAATCAA+CGG	-	chr2.2:67452625-67452644	None:intergenic	35.0%
GAATTAGCAAATTTCCGATG+AGG	-	chr2.2:67452634-67452653	None:intergenic	35.0%
GTAATCTTCGTCAGAAAGAT+TGG	+	chr2.2:67452335-67452354	MS.gene01562:CDS	35.0%
TTATTCATCACCTGCAATTG+CGG	+	chr2.2:67452660-67452679	MS.gene01562:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	67452202	67453215	67452202	ID=MS.gene01562
chr2.2	mRNA	67452202	67453215	67452202	ID=MS.gene01562.t1;Parent=MS.gene01562
chr2.2	exon	67452202	67452750	67452202	ID=MS.gene01562.t1.exon1;Parent=MS.gene01562.t1
chr2.2	CDS	67452202	67452750	67452202	ID=cds.MS.gene01562.t1;Parent=MS.gene01562.t1
chr2.2	exon	67452955	67453138	67452955	ID=MS.gene01562.t1.exon2;Parent=MS.gene01562.t1
chr2.2	CDS	67452955	67453138	67452955	ID=cds.MS.gene01562.t1;Parent=MS.gene01562.t1
chr2.2	exon	67453196	67453215	67453196	ID=MS.gene01562.t1.exon3;Parent=MS.gene01562.t1
chr2.2	CDS	67453196	67453215	67453196	ID=cds.MS.gene01562.t1;Parent=MS.gene01562.t1

Gene Sequence

>MS.gene01562

ATGCCGGATTTTCACCACGATTCTTTCACACCACCGTCACGCACCGTCCCCGTTCGCGAGGATTGTTGGTCAGAAGAAGCTTCTTCCACTCTCGTCGATGCATGGGGTCGTCGGTATCTTGAACTCAACCGTGGTAATCTTCGTCAGAAAGATTGGCAAGATGTTGCTGATGCTGTTAACGCTCTTCATGGTCATACCAAGAAAACTCATCGCACAGATGTTCAGTGTAAGAATCGAATCGATACGATTAAGAAAAAGTATAAGGTTGAAAAAGCTAGGGTTACGGAATCTAATGGTGTTTTGAATTCTAATTGGCCTTTTTTTGAACGGTTAGATGTTTTGATTGGACCTAATTTCAGTGCTAAGAAGTTATCAATTTCTTCACCGGTGGCGGTGACACCGTCGCCTCCGGTGGCTCTTCCGTTGATTCCTCATCGGAAATTTGCTAATTCTGGTGGTTATTCATCACCTGCAATTGCGGTACATTCCACCGCGGTGGCGTTACCTCAGAAGAGGAATTATTCTGCCGTGGCTGCGGCTACTGCTACGGTGATGGATGATGGGTATTTTAGGAGGAATTATTCTGCGGTAGCTGCAGCCGCTGCTGCTGCGGAAGCTGATGAGGATGATGATGAGGATGATGAGGTGGAGGAAGTTGAGGAGGAAGAGGTGGAAGTAGAGGAAGAGGAAGGGAGGGGGAGTGAAGTGGATGAAGGGGATAAAGGGAGAGAAGGGATGAAGAGGTTGGCGAAGGCGATAGAGAGTTTTGGTGAAATGTATGAGAGAGTGGAAGAACAGAAACTGAGGCAGATGGTGGATTTGGAGAAACAAAGGATGCAGTTTGCTAAGGATCTTGAAGTGCAGAGGATGCAGATGTTTATGGATACTCAAGTTCAGCTGGAGAGAATTAAGCGTGGAAAACCATCTGGGTCTAATGGTGAGATATAAAGTAAAGTAATTTAAGCTTTTTAATTGTTTTTGTTTCCCCTTTTAGCTTGTGATTTGATAATTTAA

Protein sequence

>MS.gene01562.t1

MPDFHHDSFTPPSRTVPVREDCWSEEASSTLVDAWGRRYLELNRGNLRQKDWQDVADAVNALHGHTKKTHRTDVQCKNRIDTIKKKYKVEKARVTESNGVLNSNWPFFERLDVLIGPNFSAKKLSISSPVAVTPSPPVALPLIPHRKFANSGGYSSPAIAVHSTAVALPQKRNYSAVAAATATAIESFGEMYERVEEQKLRQMVDLEKQRMQFAKDLEVQRMQMFMDTQVQLERIKRGKPSGSNACDLII