AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015660

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015660.t1 MTR_8g105200 97.308 483 12 1 1 483 1 482 0.0 959
MS.gene015660.t1 MTR_5g010630 92.233 103 5 2 198 300 5 104 1.74e-60 193
MS.gene015660.t1 MTR_2g067520 88.406 69 8 0 198 266 5 73 1.76e-37 132
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015660.t1 AT1G24610 65.962 473 156 4 11 480 4 474 0.0 626
MS.gene015660.t1 AT1G14030 24.945 453 281 19 20 459 33 439 4.81e-14 74.7

Find 111 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGATTCATCATCCATTAATT+TGG 0.191527 4.4:-2132064 None:intergenic
CAAATATGATGGTGCTCTTT+TGG 0.205619 4.4:+2130787 MS.gene015660:CDS
CTCGATTCCTCAAATGGTTT+AGG 0.230593 4.4:+2129173 MS.gene015660:CDS
ACACTTTGCCACCAAATTAA+TGG 0.235094 4.4:+2132053 MS.gene015660:CDS
CTATAATTACAGACTTCTTT+TGG 0.248268 4.4:-2132140 None:intergenic
CATCCATAGCAAAGTAATAA+AGG 0.272419 4.4:-2130692 None:intergenic
TCGATTCCTCAAATGGTTTA+GGG 0.287954 4.4:+2129174 MS.gene015660:CDS
TCCTGAACAATTCTAGCATT+TGG 0.291593 4.4:-2130439 None:intergenic
TTATTGAAGCAGGGTGCTTC+AGG 0.306570 4.4:+2132087 MS.gene015660:CDS
CCACCAAACGGATGCTTATC+TGG 0.313373 4.4:-2130265 None:intergenic
CTCAAATGGTTTAGGGTTAG+TGG 0.331608 4.4:+2129181 MS.gene015660:CDS
TCTTCCCCAGGAAAGAAAAT+AGG 0.342405 4.4:-2129615 None:intergenic
TGAATTGTGGGCAATGAAAC+TGG 0.345575 4.4:+2129509 MS.gene015660:CDS
GAGATGAAGCATCTACTTCT+TGG 0.347456 4.4:-2130288 None:intergenic
TCCAAATGCTAGAATTGTTC+AGG 0.369501 4.4:+2130438 MS.gene015660:CDS
CAATTAACCTCCCATGTTCC+CGG 0.370584 4.4:+2129305 MS.gene015660:CDS
GGAGCAGGTGTAGAGAAATT+TGG 0.377711 4.4:-2130842 None:intergenic
GAAGTAGATGCTTCATCTCT+TGG 0.384991 4.4:+2130292 MS.gene015660:CDS
CATGGTAACAAGCAATCAAA+TGG 0.388478 4.4:+2130355 MS.gene015660:CDS
CACCCACATCTTTGTGCTAC+CGG 0.389753 4.4:-2130470 None:intergenic
AGTACTCCTTGCAAGTGATA+TGG 0.393467 4.4:+2131909 MS.gene015660:CDS
CTTCATGAAAGGGCAAAACT+TGG 0.399181 4.4:+2129543 MS.gene015660:CDS
GCTCAAAATCAAGAAGAAAT+CGG 0.402032 4.4:-2130210 None:intergenic
GCAATTAGGTCAGTACCAAT+TGG 0.411464 4.4:-2129218 None:intergenic
GTGGAAGATACCTTGAGATC+TGG 0.412921 4.4:-2130911 None:intergenic
TTGGTTAATATGGTTATAGT+TGG 0.414989 4.4:+2128975 None:intergenic
CACTAACCCTAAACCATTTG+AGG 0.419235 4.4:-2129180 None:intergenic
ATCCACACGATCTCATCAAA+TGG 0.420118 4.4:+2129102 MS.gene015660:CDS
GACCCTTTATTACTTTGCTA+TGG 0.431078 4.4:+2130689 MS.gene015660:CDS
AATAATAGAAGGTCGTTTGT+TGG 0.431982 4.4:+2131876 MS.gene015660:CDS
GCACAACTCGATTCCTCAAA+TGG 0.432361 4.4:+2129167 MS.gene015660:CDS
TTAGATGCTGAGGCTCCTCT+TGG 0.432705 4.4:+2131970 MS.gene015660:CDS
ATCCATAGCAAAGTAATAAA+GGG 0.432856 4.4:-2130691 None:intergenic
TGATTACACATGTCAATTAG+AGG 0.440828 4.4:-2130406 None:intergenic
GATGAAGATGCGCATGTTAG+TGG 0.442848 4.4:-2129053 None:intergenic
GTTAATATGGTTATAGTTGG+TGG 0.443140 4.4:+2128978 None:intergenic
AAGTGTACCTGGTGAAGAAT+AGG 0.446363 4.4:-2129660 None:intergenic
TTATAGTTGGTGGTGGTAGT+TGG 0.452028 4.4:+2128988 MS.gene015660:CDS
TTCATCATCCATTAATTTGG+TGG 0.452682 4.4:-2132061 None:intergenic
TTGAGCTCGATACAGTCATA+AGG 0.453734 4.4:-2130767 None:intergenic
TTCATGTTACAGGTGAGCTT+AGG 0.456412 4.4:+2131844 MS.gene015660:intron
ACCGTCTCCTTTGATGAAAG+AGG 0.457569 4.4:-2132195 None:intergenic
TAGGAAGAGTGTAAGTTTGT+GGG 0.460771 4.4:-2129596 None:intergenic
GATGCTGCGAGCATGGCAGC+TGG 0.474563 4.4:+2130809 MS.gene015660:CDS
TGAGCTCGATACAGTCATAA+GGG 0.476129 4.4:-2130766 None:intergenic
TGATGAATCTTTATTGAAGC+AGG 0.476763 4.4:+2132077 MS.gene015660:CDS
TGTGTTGAGGAAGAGCAATT+AGG 0.483022 4.4:-2129232 None:intergenic
ATGTTACAGGTGAGCTTAGG+TGG 0.487383 4.4:+2131847 MS.gene015660:intron
ATAGGAAGAGTGTAAGTTTG+TGG 0.491387 4.4:-2129597 None:intergenic
CAATTAGGTCAGTACCAATT+GGG 0.491707 4.4:-2129217 None:intergenic
TTTGAAGCTTCTTCATGAAA+GGG 0.494538 4.4:+2129533 MS.gene015660:CDS
GTTTGAAGCTTCTTCATGAA+AGG 0.494844 4.4:+2129532 MS.gene015660:CDS
CTCTACACCTGCTCCATGGC+AGG 0.495127 4.4:+2130850 MS.gene015660:CDS
TGGTTTAGGGTTAGTGGCTA+AGG 0.496899 4.4:+2129187 MS.gene015660:CDS
ACACCAGATAAGCATCCGTT+TGG 0.499859 4.4:+2130262 MS.gene015660:CDS
GCTAAGGAAGATATCCCAAT+TGG 0.505887 4.4:+2129203 MS.gene015660:CDS
TGTGGGAGATTACTGATGTA+TGG 0.507516 4.4:-2129579 None:intergenic
GCATTTGGCAGATGAATTGT+GGG 0.511625 4.4:+2129497 MS.gene015660:intron
CCAGATAAGCATCCGTTTGG+TGG 0.512174 4.4:+2130265 MS.gene015660:CDS
TCAAGAGCATTCAAGTTACA+TGG 0.514609 4.4:+2130337 MS.gene015660:CDS
CTATCTCAGCTAAATCTAGC+TGG 0.514942 4.4:+2130881 MS.gene015660:CDS
GGTGGGCAAGAAATAATAGA+AGG 0.517366 4.4:+2131865 MS.gene015660:CDS
TAGATGCTTCATCTCTTGGA+TGG 0.521095 4.4:+2130296 MS.gene015660:CDS
ATAAGTTCCTGCCATGGAGC+AGG 0.528390 4.4:-2130857 None:intergenic
AATGACACACAGAGCAATCA+AGG 0.529045 4.4:-2132022 None:intergenic
AATATGGTTATAGTTGGTGG+TGG 0.531556 4.4:+2128981 None:intergenic
TCAATTAGAGGGAGCATCAT+GGG 0.532994 4.4:-2130394 None:intergenic
TCCACACGATCTCATCAAAT+GGG 0.536822 4.4:+2129103 MS.gene015660:CDS
TGGTGGTGGTAGTTGGAAAA+CGG 0.543989 4.4:+2128995 MS.gene015660:CDS
GTCAATTAGAGGGAGCATCA+TGG 0.544285 4.4:-2130395 None:intergenic
ATGGTAACAAGCAATCAAAT+GGG 0.547291 4.4:+2130356 MS.gene015660:CDS
GAGGCTTTACCTTCCTTGAG+AGG 0.548939 4.4:-2132176 None:intergenic
TCACAAATCCATAATCAAGA+AGG 0.549483 4.4:-2130733 None:intergenic
TCTACTTCTTGGCCACCAAA+CGG 0.549674 4.4:-2130277 None:intergenic
ATCAAATGGGTAACAAGAGA+AGG 0.550397 4.4:+2129116 MS.gene015660:CDS
TGTGGCTGAGAAAGCAATCA+AGG 0.555448 4.4:+2130661 MS.gene015660:CDS
AGCATTTGGCAGATGAATTG+TGG 0.556575 4.4:+2129496 MS.gene015660:intron
GGTAAAGCCTCTTTCATCAA+AGG 0.561143 4.4:+2132188 MS.gene015660:CDS
CTATGCTCCTATTCTTCACC+AGG 0.562752 4.4:+2129653 MS.gene015660:CDS
AAGCCGGTAGCACAAAGATG+TGG 0.568257 4.4:+2130467 MS.gene015660:CDS
TGGAGAAACTCCAGATCTCA+AGG 0.573409 4.4:+2130901 MS.gene015660:CDS
AAGGAGACGGTCACTACTGA+AGG 0.581558 4.4:+2132207 MS.gene015660:CDS
GCCTCTTTCATCAAAGGAGA+CGG 0.582197 4.4:+2132194 MS.gene015660:CDS
GATGAATCTTTATTGAAGCA+GGG 0.583312 4.4:+2132078 MS.gene015660:CDS
CACTTGCCATATCACTTGCA+AGG 0.586602 4.4:-2131915 None:intergenic
CATGGGAGGTTAATTGAAGG+AGG 0.592569 4.4:-2129298 None:intergenic
AAATTCTTGATGTCTTCCCC+AGG 0.592666 4.4:-2129627 None:intergenic
TTGATGAGATCGTGTGGATG+AGG 0.593115 4.4:-2129098 None:intergenic
AAAGTCAAGCATGCATTAGT+CGG 0.594145 4.4:+2130235 MS.gene015660:CDS
GCTCTGTGTGTCATTGCACT+AGG 0.596790 4.4:+2132030 MS.gene015660:CDS
GAACATGGGAGGTTAATTGA+AGG 0.597020 4.4:-2129301 None:intergenic
GATTACACATGTCAATTAGA+GGG 0.597579 4.4:-2130405 None:intergenic
ACTTAAGTCTTTAGATGCTG+AGG 0.601367 4.4:+2131960 MS.gene015660:CDS
AAGTGCAGAGGAATGTGTTG+AGG 0.602415 4.4:-2129245 None:intergenic
TGTTACAGGTGAGCTTAGGT+GGG 0.606576 4.4:+2131848 MS.gene015660:intron
CTAAAGACTTAAGTGTGTTG+AGG 0.608231 4.4:-2131951 None:intergenic
AAATGGGTAACAAGAGAAGG+TGG 0.609234 4.4:+2129119 MS.gene015660:CDS
AGATGCTTCATCTCTTGGAT+GGG 0.610544 4.4:+2130297 MS.gene015660:CDS
ATTTCTCTACACCTGCTCCA+TGG 0.612721 4.4:+2130846 MS.gene015660:CDS
TAGCACAAAGATGTGGGTGA+AGG 0.614670 4.4:+2130474 MS.gene015660:CDS
AGCCGGTAGCACAAAGATGT+GGG 0.619848 4.4:+2130468 MS.gene015660:CDS
ATTGTTCAGGAACAAGAAGC+CGG 0.621234 4.4:+2130451 MS.gene015660:CDS
GATAGAATAAGTTCCTGCCA+TGG 0.632180 4.4:-2130863 None:intergenic
TCATCATCGTTGAAGTGCAG+AGG 0.633506 4.4:-2129257 None:intergenic
GAATTGTGGGCAATGAAACT+GGG 0.634097 4.4:+2129510 MS.gene015660:CDS
TGTATCGAGCTCAAATATGA+TGG 0.639565 4.4:+2130776 MS.gene015660:CDS
AGGTCATTTGCAACACCAAG+AGG 0.646673 4.4:-2131985 None:intergenic
TTACTTACCGGGAACATGGG+AGG 0.654333 4.4:-2129312 None:intergenic
ACCCATTTGATGAGATCGTG+TGG 0.664082 4.4:-2129104 None:intergenic
TCAAGGTACGAAATACAGCA+AGG 0.687972 4.4:-2132005 None:intergenic
GTTGTGCTATCTTCACAGCA+CGG 0.695139 4.4:-2129151 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATTTATTAACCATTCAA+TGG - chr4.4:2131112-2131131 None:intergenic 15.0%
!! TTAAAAATGAAAACTACTAT+TGG + chr4.4:2130966-2130985 MS.gene015660:intron 15.0%
!!! AATGTTTTTATAGCAAATAT+TGG + chr4.4:2131653-2131672 MS.gene015660:intron 15.0%
!!! TTCAAATATACATGATTTTA+AGG - chr4.4:2131277-2131296 None:intergenic 15.0%
!! AAAAATAGTATAATGCTTTG+TGG + chr4.4:2129725-2129744 MS.gene015660:intron 20.0%
!! AACATAATGCGTAAAAATAA+TGG - chr4.4:2131581-2131600 None:intergenic 20.0%
!! AGAAATTCAATATTTACCTT+AGG + chr4.4:2130637-2130656 MS.gene015660:intron 20.0%
!! TAAATCAAATTATGACCTTA+TGG - chr4.4:2131819-2131838 None:intergenic 20.0%
!! TTTAGATCGTTAAGTATATA+TGG - chr4.4:2131432-2131451 None:intergenic 20.0%
!!! AATTTTTTAGTAAAAACCAG+TGG + chr4.4:2129797-2129816 MS.gene015660:intron 20.0%
!!! ATTTACTTTTTTCATGTTAC+AGG + chr4.4:2131834-2131853 MS.gene015660:intron 20.0%
!!! GTCATTATACATAATTTTTC+AGG - chr4.4:2131360-2131379 None:intergenic 20.0%
! AAATTGCTCATTTCAAAAGT+GGG - chr4.4:2130046-2130065 None:intergenic 25.0%
! ACCAAAACTTTCATATCAAT+TGG + chr4.4:2131775-2131794 MS.gene015660:intron 25.0%
! AGATTCATCATCCATTAATT+TGG - chr4.4:2132067-2132086 None:intergenic 25.0%
! ATAACTAAATCGCGATAATT+TGG - chr4.4:2130015-2130034 None:intergenic 25.0%
! ATCCATAGCAAAGTAATAAA+GGG - chr4.4:2130694-2130713 None:intergenic 25.0%
! ATGTATATTTGAATCCAGTT+TGG + chr4.4:2131284-2131303 MS.gene015660:intron 25.0%
! CAGTTTGGATGATTAATATA+TGG + chr4.4:2131299-2131318 MS.gene015660:intron 25.0%
! CATATATTAATCATCCAAAC+TGG - chr4.4:2131301-2131320 None:intergenic 25.0%
! GAGATTAATGACATTCAAAA+TGG - chr4.4:2131187-2131206 None:intergenic 25.0%
! TAAAAACCAGTGGTAAAATT+CGG + chr4.4:2129807-2129826 MS.gene015660:intron 25.0%
! TAATTAATAGACACTGAGAT+AGG - chr4.4:2129968-2129987 None:intergenic 25.0%
! TTCATAGTTATTCTATACGA+AGG - chr4.4:2131623-2131642 None:intergenic 25.0%
!! AATACAGCTATAGAATTTTG+TGG - chr4.4:2130933-2130952 None:intergenic 25.0%
!! ACCAATTGATATGAAAGTTT+TGG - chr4.4:2131779-2131798 None:intergenic 25.0%
!! ATATCTCCAATTGTAGTTTT+GGG + chr4.4:2130079-2130098 MS.gene015660:intron 25.0%
!! CTATAATTACAGACTTCTTT+TGG - chr4.4:2132143-2132162 None:intergenic 25.0%
!! TATATCTCCAATTGTAGTTT+TGG + chr4.4:2130078-2130097 MS.gene015660:intron 25.0%
!!! ACTTTTGAAATGAGCAATTT+GGG + chr4.4:2130046-2130065 MS.gene015660:intron 25.0%
!!! ATATGAAAGTTTTGGTAAGT+TGG - chr4.4:2131771-2131790 None:intergenic 25.0%
!!! TAATTATTTTGAATGCCTTG+CGG + chr4.4:2130124-2130143 MS.gene015660:intron 25.0%
!!! TATGAAAGTTTTGGTAAGTT+GGG - chr4.4:2131770-2131789 None:intergenic 25.0%
!!! TGCGTAAAAATAATGGATTA+TGG - chr4.4:2131574-2131593 None:intergenic 25.0%
AATAATAGAAGGTCGTTTGT+TGG + chr4.4:2131876-2131895 MS.gene015660:CDS 30.0%
AATTGCTCATTTCAAAAGTG+GGG - chr4.4:2130045-2130064 None:intergenic 30.0%
ACAATTGGAGATATACTATG+CGG - chr4.4:2130073-2130092 None:intergenic 30.0%
ACATTCAAAATGGTCGTTAA+CGG - chr4.4:2131177-2131196 None:intergenic 30.0%
ATATATGTGTTTGTGTGTGT+TGG + chr4.4:2131330-2131349 MS.gene015660:intron 30.0%
ATGGTAACAAGCAATCAAAT+GGG + chr4.4:2130356-2130375 MS.gene015660:CDS 30.0%
ATGTAATGAAAAACCTCTCA+AGG + chr4.4:2132163-2132182 MS.gene015660:CDS 30.0%
CAAATTGCTCATTTCAAAAG+TGG - chr4.4:2130047-2130066 None:intergenic 30.0%
CATCCATAGCAAAGTAATAA+AGG - chr4.4:2130695-2130714 None:intergenic 30.0%
CTCAGTGTCTATTAATTATG+TGG + chr4.4:2129970-2129989 MS.gene015660:intron 30.0%
GATGAATCTTTATTGAAGCA+GGG + chr4.4:2132078-2132097 MS.gene015660:CDS 30.0%
GATTACACATGTCAATTAGA+GGG - chr4.4:2130408-2130427 None:intergenic 30.0%
GCTCAAAATCAAGAAGAAAT+CGG - chr4.4:2130213-2130232 None:intergenic 30.0%
GTATGTTTGAACACAAATTG+TGG + chr4.4:2131220-2131239 MS.gene015660:intron 30.0%
GTTGAATAAATTGGACAATG+TGG + chr4.4:2129472-2129491 MS.gene015660:intron 30.0%
TCACAAATCCATAATCAAGA+AGG - chr4.4:2130736-2130755 None:intergenic 30.0%
TCGGTGTAAATAATGTTAGT+AGG + chr4.4:2129826-2129845 MS.gene015660:intron 30.0%
TGAATAAGTTAAGAGGATTC+AGG - chr4.4:2131721-2131740 None:intergenic 30.0%
TGATGAATCTTTATTGAAGC+AGG + chr4.4:2132077-2132096 MS.gene015660:CDS 30.0%
TGATTACACATGTCAATTAG+AGG - chr4.4:2130409-2130428 None:intergenic 30.0%
! GATCTTTTCCTTCTTGATTA+TGG + chr4.4:2130725-2130744 MS.gene015660:CDS 30.0%
! TCAATATTTACCTTAGGTTG+TGG + chr4.4:2130643-2130662 MS.gene015660:intron 30.0%
! TTCATCATCCATTAATTTGG+TGG - chr4.4:2132064-2132083 None:intergenic 30.0%
! TTTGAAGCTTCTTCATGAAA+GGG + chr4.4:2129533-2129552 MS.gene015660:CDS 30.0%
!! TATCTCCAATTGTAGTTTTG+GGG + chr4.4:2130080-2130099 MS.gene015660:intron 30.0%
!! TTTCTTTCTAGAGTCTTGTT+TGG - chr4.4:2129420-2129439 None:intergenic 30.0%
!!! CACTTTTGAAATGAGCAATT+TGG + chr4.4:2130045-2130064 MS.gene015660:intron 30.0%
AAAGTCAAGCATGCATTAGT+CGG + chr4.4:2130235-2130254 MS.gene015660:CDS 35.0%
AAATTCATGAATCACGAGAC+AGG + chr4.4:2130174-2130193 MS.gene015660:intron 35.0%
AACAGTAAGTAAAGTGTACC+TGG - chr4.4:2129674-2129693 None:intergenic 35.0%
AATGAAAAACCTCTCAAGGA+AGG + chr4.4:2132167-2132186 MS.gene015660:CDS 35.0%
AATTCATGAGCTAATTGAGC+TGG - chr4.4:2129343-2129362 None:intergenic 35.0%
ACACTTTGCCACCAAATTAA+TGG + chr4.4:2132053-2132072 MS.gene015660:CDS 35.0%
ACTTAAGTCTTTAGATGCTG+AGG + chr4.4:2131960-2131979 MS.gene015660:CDS 35.0%
ATCAAATGGGTAACAAGAGA+AGG + chr4.4:2129116-2129135 MS.gene015660:CDS 35.0%
ATGTTAGCACAAGAACCATA+AGG + chr4.4:2131801-2131820 MS.gene015660:intron 35.0%
ATTGCTCATTTCAAAAGTGG+GGG - chr4.4:2130044-2130063 None:intergenic 35.0%
CAAGGGTTGAATAAGTTAAG+AGG - chr4.4:2131728-2131747 None:intergenic 35.0%
CAATTAGGTCAGTACCAATT+GGG - chr4.4:2129220-2129239 None:intergenic 35.0%
CATGGTAACAAGCAATCAAA+TGG + chr4.4:2130355-2130374 MS.gene015660:CDS 35.0%
CTAAAGACTTAAGTGTGTTG+AGG - chr4.4:2131954-2131973 None:intergenic 35.0%
CTCTTAACTTATTCAACCCT+TGG + chr4.4:2131726-2131745 MS.gene015660:intron 35.0%
GACCCTTTATTACTTTGCTA+TGG + chr4.4:2130689-2130708 MS.gene015660:CDS 35.0%
GGTTAATTACGTCCATTGAA+TGG + chr4.4:2131097-2131116 MS.gene015660:intron 35.0%
GTCGTCGATGTTGAATAAAT+TGG + chr4.4:2129463-2129482 MS.gene015660:intron 35.0%
TCCAAATGCTAGAATTGTTC+AGG + chr4.4:2130438-2130457 MS.gene015660:CDS 35.0%
TCCTGAACAATTCTAGCATT+TGG - chr4.4:2130442-2130461 None:intergenic 35.0%
TCGATTCCTCAAATGGTTTA+GGG + chr4.4:2129174-2129193 MS.gene015660:CDS 35.0%
TGTATCGAGCTCAAATATGA+TGG + chr4.4:2130776-2130795 MS.gene015660:CDS 35.0%
! AATTTTACTTACCGGGAACA+TGG - chr4.4:2129319-2129338 None:intergenic 35.0%
! ACTCTTCCTATTTTCTTTCC+TGG + chr4.4:2129609-2129628 MS.gene015660:CDS 35.0%
! AGCTGGTAATTTTACTTACC+GGG - chr4.4:2129326-2129345 None:intergenic 35.0%
! ATAGGAAGAGTGTAAGTTTG+TGG - chr4.4:2129600-2129619 None:intergenic 35.0%
! ATTTTACTTACCGGGAACAT+GGG - chr4.4:2129318-2129337 None:intergenic 35.0%
! CAAAACTTGGATCTTTTTGG+TGG + chr4.4:2129556-2129575 MS.gene015660:CDS 35.0%
! CTCTTCCTATTTTCTTTCCT+GGG + chr4.4:2129610-2129629 MS.gene015660:CDS 35.0%
! CTTTTGGATTCTGAACTGAA+TGG - chr4.4:2132127-2132146 None:intergenic 35.0%
! GAGCTGGTAATTTTACTTAC+CGG - chr4.4:2129327-2129346 None:intergenic 35.0%
! GTTTGAAGCTTCTTCATGAA+AGG + chr4.4:2129532-2129551 MS.gene015660:CDS 35.0%
! TAGGAAGAGTGTAAGTTTGT+GGG - chr4.4:2129599-2129618 None:intergenic 35.0%
! TCTTCCTATTTTCTTTCCTG+GGG + chr4.4:2129611-2129630 MS.gene015660:CDS 35.0%
! TGTTTTCCCTCTGTTCATTA+AGG + chr4.4:2131597-2131616 MS.gene015660:intron 35.0%
! TTTACACCGAATTTTACCAC+TGG - chr4.4:2129816-2129835 None:intergenic 35.0%
!! CAAATATGATGGTGCTCTTT+TGG + chr4.4:2130787-2130806 MS.gene015660:CDS 35.0%
!! TCAAGAGCATTCAAGTTACA+TGG + chr4.4:2130337-2130356 MS.gene015660:CDS 35.0%
AAATGGGTAACAAGAGAAGG+TGG + chr4.4:2129119-2129138 MS.gene015660:CDS 40.0%
AAATTCTTGATGTCTTCCCC+AGG - chr4.4:2129630-2129649 None:intergenic 40.0%
AAGTGTACCTGGTGAAGAAT+AGG - chr4.4:2129663-2129682 None:intergenic 40.0%
AATGACACACAGAGCAATCA+AGG - chr4.4:2132025-2132044 None:intergenic 40.0%
AGATAGGCAATTGTAGCTTC+TGG - chr4.4:2129952-2129971 None:intergenic 40.0%
AGATGCTTCATCTCTTGGAT+GGG + chr4.4:2130297-2130316 MS.gene015660:CDS 40.0%
AGCATTTGGCAGATGAATTG+TGG + chr4.4:2129496-2129515 MS.gene015660:intron 40.0%
AGTACTCCTTGCAAGTGATA+TGG + chr4.4:2131909-2131928 MS.gene015660:CDS 40.0%
ATCCACACGATCTCATCAAA+TGG + chr4.4:2129102-2129121 MS.gene015660:CDS 40.0%
ATTGTTCAGGAACAAGAAGC+CGG + chr4.4:2130451-2130470 MS.gene015660:CDS 40.0%
CACTAACCCTAAACCATTTG+AGG - chr4.4:2129183-2129202 None:intergenic 40.0%
CTATCTCAGCTAAATCTAGC+TGG + chr4.4:2130881-2130900 MS.gene015660:CDS 40.0%
CTCGATTCCTCAAATGGTTT+AGG + chr4.4:2129173-2129192 MS.gene015660:CDS 40.0%
CTTCATGAAAGGGCAAAACT+TGG + chr4.4:2129543-2129562 MS.gene015660:CDS 40.0%
GAACATGGGAGGTTAATTGA+AGG - chr4.4:2129304-2129323 None:intergenic 40.0%
GAAGTAGATGCTTCATCTCT+TGG + chr4.4:2130292-2130311 MS.gene015660:CDS 40.0%
GAATTGTGGGCAATGAAACT+GGG + chr4.4:2129510-2129529 MS.gene015660:CDS 40.0%
GATAGAATAAGTTCCTGCCA+TGG - chr4.4:2130866-2130885 None:intergenic 40.0%
GCAATTAGGTCAGTACCAAT+TGG - chr4.4:2129221-2129240 None:intergenic 40.0%
GCATTTGGCAGATGAATTGT+GGG + chr4.4:2129497-2129516 MS.gene015660:intron 40.0%
GCTAAGGAAGATATCCCAAT+TGG + chr4.4:2129203-2129222 MS.gene015660:CDS 40.0%
GCTTACTGTACTTTGCAGAA+AGG + chr4.4:2131512-2131531 MS.gene015660:intron 40.0%
GGTAAAGCCTCTTTCATCAA+AGG + chr4.4:2132188-2132207 MS.gene015660:CDS 40.0%
GGTGGGCAAGAAATAATAGA+AGG + chr4.4:2131865-2131884 MS.gene015660:CDS 40.0%
GTTAGCGCTGTTAAAGTATG+TGG + chr4.4:2131076-2131095 MS.gene015660:intron 40.0%
TAGATGCTTCATCTCTTGGA+TGG + chr4.4:2130296-2130315 MS.gene015660:CDS 40.0%
TAGTATTGTCTCTTTCACCG+AGG + chr4.4:2131689-2131708 MS.gene015660:intron 40.0%
TCAAGGTACGAAATACAGCA+AGG - chr4.4:2132008-2132027 None:intergenic 40.0%
TCCACACGATCTCATCAAAT+GGG + chr4.4:2129103-2129122 MS.gene015660:CDS 40.0%
TCTTCCCCAGGAAAGAAAAT+AGG - chr4.4:2129618-2129637 None:intergenic 40.0%
TGAATTGTGGGCAATGAAAC+TGG + chr4.4:2129509-2129528 MS.gene015660:CDS 40.0%
TGACACCCCAAAACTACAAT+TGG - chr4.4:2130088-2130107 None:intergenic 40.0%
TGAGCTCGATACAGTCATAA+GGG - chr4.4:2130769-2130788 None:intergenic 40.0%
TGTGGGAGATTACTGATGTA+TGG - chr4.4:2129582-2129601 None:intergenic 40.0%
TGTGTTGAGGAAGAGCAATT+AGG - chr4.4:2129235-2129254 None:intergenic 40.0%
TTCATGTTACAGGTGAGCTT+AGG + chr4.4:2131844-2131863 MS.gene015660:intron 40.0%
TTGAGCTCGATACAGTCATA+AGG - chr4.4:2130770-2130789 None:intergenic 40.0%
TTGGACAATGTGGTAGCATT+TGG + chr4.4:2129482-2129501 MS.gene015660:intron 40.0%
! CTCAAATGGTTTAGGGTTAG+TGG + chr4.4:2129181-2129200 MS.gene015660:CDS 40.0%
! GAGATGAAGCATCTACTTCT+TGG - chr4.4:2130291-2130310 None:intergenic 40.0%
! GGGCAAAACTTGGATCTTTT+TGG + chr4.4:2129553-2129572 MS.gene015660:CDS 40.0%
!! TCAATTAGAGGGAGCATCAT+GGG - chr4.4:2130397-2130416 None:intergenic 40.0%
!! TTATAGTTGGTGGTGGTAGT+TGG + chr4.4:2128988-2129007 MS.gene015660:CDS 40.0%
AAGTGCAGAGGAATGTGTTG+AGG - chr4.4:2129248-2129267 None:intergenic 45.0%
ACACCAGATAAGCATCCGTT+TGG + chr4.4:2130262-2130281 MS.gene015660:CDS 45.0%
ACATTGCTCTTGTGAGGACT+GGG - chr4.4:2129866-2129885 None:intergenic 45.0%
ACCCATTTGATGAGATCGTG+TGG - chr4.4:2129107-2129126 None:intergenic 45.0%
ACCGTCTCCTTTGATGAAAG+AGG - chr4.4:2132198-2132217 None:intergenic 45.0%
ACGAAGGCCTTAATGAACAG+AGG - chr4.4:2131607-2131626 None:intergenic 45.0%
AGGTCATTTGCAACACCAAG+AGG - chr4.4:2131988-2132007 None:intergenic 45.0%
AGTTGCACATTGCTCTTGTG+AGG - chr4.4:2129872-2129891 None:intergenic 45.0%
ATATGCACTGCAGTGAAGAG+AGG - chr4.4:2129751-2129770 None:intergenic 45.0%
ATGTTACAGGTGAGCTTAGG+TGG + chr4.4:2131847-2131866 MS.gene015660:intron 45.0%
ATTTCTCTACACCTGCTCCA+TGG + chr4.4:2130846-2130865 MS.gene015660:CDS 45.0%
CAATTAACCTCCCATGTTCC+CGG + chr4.4:2129305-2129324 MS.gene015660:CDS 45.0%
CACTTGCCATATCACTTGCA+AGG - chr4.4:2131918-2131937 None:intergenic 45.0%
CATGGGAGGTTAATTGAAGG+AGG - chr4.4:2129301-2129320 None:intergenic 45.0%
CGAAGGCCTTAATGAACAGA+GGG - chr4.4:2131606-2131625 None:intergenic 45.0%
CTATGCTCCTATTCTTCACC+AGG + chr4.4:2129653-2129672 MS.gene015660:CDS 45.0%
GATGAAGATGCGCATGTTAG+TGG - chr4.4:2129056-2129075 None:intergenic 45.0%
GCAATTGTAGCTTCTGGTGT+TGG - chr4.4:2129946-2129965 None:intergenic 45.0%
GCACAACTCGATTCCTCAAA+TGG + chr4.4:2129167-2129186 MS.gene015660:CDS 45.0%
GCCTCTTTCATCAAAGGAGA+CGG + chr4.4:2132194-2132213 MS.gene015660:CDS 45.0%
GGAGCAGGTGTAGAGAAATT+TGG - chr4.4:2130845-2130864 None:intergenic 45.0%
GTGGAAGATACCTTGAGATC+TGG - chr4.4:2130914-2130933 None:intergenic 45.0%
GTTGTGCTATCTTCACAGCA+CGG - chr4.4:2129154-2129173 None:intergenic 45.0%
TAGCACAAAGATGTGGGTGA+AGG + chr4.4:2130474-2130493 MS.gene015660:CDS 45.0%
TCATCATCGTTGAAGTGCAG+AGG - chr4.4:2129260-2129279 None:intergenic 45.0%
TCTACTTCTTGGCCACCAAA+CGG - chr4.4:2130280-2130299 None:intergenic 45.0%
TGGAGAAACTCCAGATCTCA+AGG + chr4.4:2130901-2130920 MS.gene015660:CDS 45.0%
TGTGGCTGAGAAAGCAATCA+AGG + chr4.4:2130661-2130680 MS.gene015660:CDS 45.0%
TGTTACAGGTGAGCTTAGGT+GGG + chr4.4:2131848-2131867 MS.gene015660:intron 45.0%
TTGATGAGATCGTGTGGATG+AGG - chr4.4:2129101-2129120 None:intergenic 45.0%
! GAGGATTCAGGTTCAAACCT+CGG - chr4.4:2131709-2131728 None:intergenic 45.0%
! TGGTTTAGGGTTAGTGGCTA+AGG + chr4.4:2129187-2129206 MS.gene015660:CDS 45.0%
! TTATTGAAGCAGGGTGCTTC+AGG + chr4.4:2132087-2132106 MS.gene015660:CDS 45.0%
!! AGCACAACTGATTTGAGCCA+AGG - chr4.4:2131746-2131765 None:intergenic 45.0%
!! CTGGTGTTGGTCACAAATGT+TGG - chr4.4:2129933-2129952 None:intergenic 45.0%
!! GCACAACTGATTTGAGCCAA+GGG - chr4.4:2131745-2131764 None:intergenic 45.0%
!! GTCAATTAGAGGGAGCATCA+TGG - chr4.4:2130398-2130417 None:intergenic 45.0%
!! TGGTGGTGGTAGTTGGAAAA+CGG + chr4.4:2128995-2129014 MS.gene015660:CDS 45.0%
AAGCCGGTAGCACAAAGATG+TGG + chr4.4:2130467-2130486 MS.gene015660:CDS 50.0%
AAGGAGACGGTCACTACTGA+AGG + chr4.4:2132207-2132226 MS.gene015660:CDS 50.0%
AGCCGGTAGCACAAAGATGT+GGG + chr4.4:2130468-2130487 MS.gene015660:CDS 50.0%
ATAAGTTCCTGCCATGGAGC+AGG - chr4.4:2130860-2130879 None:intergenic 50.0%
CACATTGCTCTTGTGAGGAC+TGG - chr4.4:2129867-2129886 None:intergenic 50.0%
CACCCACATCTTTGTGCTAC+CGG - chr4.4:2130473-2130492 None:intergenic 50.0%
CCACCAAACGGATGCTTATC+TGG - chr4.4:2130268-2130287 None:intergenic 50.0%
CCAGATAAGCATCCGTTTGG+TGG + chr4.4:2130265-2130284 MS.gene015660:CDS 50.0%
GAGGCTTTACCTTCCTTGAG+AGG - chr4.4:2132179-2132198 None:intergenic 50.0%
GCTTTCTCAGCCACAACCTA+AGG - chr4.4:2130656-2130675 None:intergenic 50.0%
GTGTGTGTGTGTGTGTGTCT+GGG - chr4.4:2130536-2130555 None:intergenic 50.0%
TGCCAAACTGCAAATCCGCA+AGG - chr4.4:2130142-2130161 None:intergenic 50.0%
TGCCTTGCGGATTTGCAGTT+TGG + chr4.4:2130137-2130156 MS.gene015660:intron 50.0%
TGTGTGTGTGTGTGTGTGTC+TGG - chr4.4:2130537-2130556 None:intergenic 50.0%
TTACTTACCGGGAACATGGG+AGG - chr4.4:2129315-2129334 None:intergenic 50.0%
TTAGATGCTGAGGCTCCTCT+TGG + chr4.4:2131970-2131989 MS.gene015660:CDS 50.0%
! GCTCTGTGTGTCATTGCACT+AGG + chr4.4:2132030-2132049 MS.gene015660:CDS 50.0%
! TCTTTTGGATGCTGCGAGCA+TGG + chr4.4:2130802-2130821 MS.gene015660:CDS 50.0%
GTCTGGGAGAGGAGTCATGA+AGG - chr4.4:2130520-2130539 None:intergenic 55.0%
TGTGTGTGTGTGTCTGGGAG+AGG - chr4.4:2130531-2130550 None:intergenic 55.0%
CTCTACACCTGCTCCATGGC+AGG + chr4.4:2130850-2130869 MS.gene015660:CDS 60.0%
GATGCTGCGAGCATGGCAGC+TGG + chr4.4:2130809-2130828 MS.gene015660:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 2128984 2132233 2128984 ID=MS.gene015660
chr4.4 mRNA 2128984 2132233 2128984 ID=MS.gene015660.t1;Parent=MS.gene015660
chr4.4 exon 2128984 2129326 2128984 ID=MS.gene015660.t1.exon1;Parent=MS.gene015660.t1
chr4.4 CDS 2128984 2129326 2128984 ID=cds.MS.gene015660.t1;Parent=MS.gene015660.t1
chr4.4 exon 2129508 2129674 2129508 ID=MS.gene015660.t1.exon2;Parent=MS.gene015660.t1
chr4.4 CDS 2129508 2129674 2129508 ID=cds.MS.gene015660.t1;Parent=MS.gene015660.t1
chr4.4 exon 2130196 2130495 2130196 ID=MS.gene015660.t1.exon3;Parent=MS.gene015660.t1
chr4.4 CDS 2130196 2130495 2130196 ID=cds.MS.gene015660.t1;Parent=MS.gene015660.t1
chr4.4 exon 2130659 2130922 2130659 ID=MS.gene015660.t1.exon4;Parent=MS.gene015660.t1
chr4.4 CDS 2130659 2130922 2130659 ID=cds.MS.gene015660.t1;Parent=MS.gene015660.t1
chr4.4 exon 2131856 2132233 2131856 ID=MS.gene015660.t1.exon5;Parent=MS.gene015660.t1
chr4.4 CDS 2131856 2132233 2131856 ID=cds.MS.gene015660.t1;Parent=MS.gene015660.t1
Gene Sequence

>MS.gene015660

ATGGTTATAGTTGGTGGTGGTAGTTGGAAAACGGCGATGTTAATGTCATCGTCGTTGAGTCACGTGCGTCCACTAACATGCGCATCTTCATCTTCATCATCATCACGAGTAGCTCCTCATCCACACGATCTCATCAAATGGGTAACAAGAGAAGGTGGATTCGTTCACCGTGCTGTGAAGATAGCACAACTCGATTCCTCAAATGGTTTAGGGTTAGTGGCTAAGGAAGATATCCCAATTGGTACTGACCTAATTGCTCTTCCTCAACACATTCCTCTGCACTTCAACGATGATGATCGCAACTCTCACTCTCTCCTCCTTCAATTAACCTCCCATGTTCCCGGTAAGTAAAATTACCAGCTCAATTAGCTCATGAATTTGTATCTGTATGATAAGAAGAATTTATGTTATAAGCATTTAATTAAATGTTTATCCAAACAAGACTCTAGAAAGAAATAACATACATATAGTATTGAGTTGTCGTCGATGTTGAATAAATTGGACAATGTGGTAGCATTTGGCAGATGAATTGTGGGCAATGAAACTGGGTTTGAAGCTTCTTCATGAAAGGGCAAAACTTGGATCTTTTTGGTGGCCATACATCAGTAATCTCCCACAAACTTACACTCTTCCTATTTTCTTTCCTGGGGAAGACATCAAGAATTTGCACTATGCTCCTATTCTTCACCAGGTACACTTTACTTACTGTTAATCATTTTCGACTATACTATGTTATATGAAAAAAATAGTATAATGCTTTGTGGCCTCTCTTCACTGCAGTGCATATTGCTGATCAAGAAAAGAAATGCATACAATTTTTTAGTAAAAACCAGTGGTAAAATTCGGTGTAAATAATGTTAGTAGGTTTTTATTACTTCTCCCAGTCCTCACAAGAGCAATGTGCAACTTTGTACTCTTTGAAACATTTGAAATATTCTTAAGATATCCAACATTTGTGACCAACACCAGAAGCTACAATTGCCTATCTCAGTGTCTATTAATTATGTGGCTTAATTGCACTTTTGACTCCAAATTATCGCGATTTAGTTATTTTTGTCCCCCACTTTTGAAATGAGCAATTTGGGTCCGCATAGTATATCTCCAATTGTAGTTTTGGGGTGTCAAAAATGCAATTAAGTCTAATTATTTTGAATGCCTTGCGGATTTGCAGTTTGGCAGCTAATTTTGTCAAATTCATGAATCACGAGACAGGTAAACAAAAGATGCCGATTTCTTCTTGATTTTGAGCAAAAAGTCAAGCATGCATTAGTCGGTCTTACACCAGATAAGCATCCGTTTGGTGGCCAAGAAGTAGATGCTTCATCTCTTGGATGGGCGATGTCAGCAGTTTCATCAAGAGCATTCAAGTTACATGGTAACAAGCAATCAAATGGGATAAACTTTGACATTCCCATGATGCTCCCTCTAATTGACATGTGTAATCATAGTTTCAATCCAAATGCTAGAATTGTTCAGGAACAAGAAGCCGGTAGCACAAAGATGTGGGTGAAGGCATGTTATTTCATACTCTTTCCTTCATGACTCCTCTCCCAGACACACACACACACACACACACACACACACACACACACACACGCTCACACGTCAAATTCTGCTTTTAGAAACAACTATTCTTTAGATTTTTCTTTGCTGAGAAATTCAATATTTACCTTAGGTTGTGGCTGAGAAAGCAATCAAGGAAGATGACCCTTTATTACTTTGCTATGGATGTTTAAGCAATGATCTTTTCCTTCTTGATTATGGATTTGTGATACAGTCAAACCCTTATGACTGTATCGAGCTCAAATATGATGGTGCTCTTTTGGATGCTGCGAGCATGGCAGCTGGAGTTTCTTCACCAAATTTCTCTACACCTGCTCCATGGCAGGAACTTATTCTATCTCAGCTAAATCTAGCTGGAGAAACTCCAGATCTCAAGGTATCTTCCACAAAATTCTATAGCTGTATTTAATTACTACTATTTAAAAATGAAAACTACTATTGGATGTTAAATAAGTGCTCTTACTCTTTCTATTAAAGAAATGACATTTTGACACACAAATTTGACAAAATTTTGACAAATATTTAGAATGTTAGCGCTGTTAAAGTATGTGGTTAATTACGTCCATTGAATGGTTAATAAATATAAATATAACAAATTGTTGCAATTCATTAATGATATACTCAACACCGTTAACGACCATTTTGAATGTCATTAATCTCAATGTTCATTTGAGTATGTTTGAACACAAATTGTGGTTAATGAATATCATATATAAATATTTGAACACCTTAAAATCATGTATATTTGAATCCAGTTTGGATGATTAATATATGGATATATGAATATATGTGTTTGTGTGTGTTGGAAAACCTGAAAAATTATGTATAATGACGTTCAAAATCGCAGTTTATTTTCATCAATCATCTAATGAATGTAATTGACCATATATACTTAACGATCTAAATTGTTTAATTTTGATGTCAAGTTTGTATGTCAAAAAAACACATCTCTTTCTATTATATAAGCTTACTGTACTTTGCAGAAAGGTTTAATCTCTTCTTTGTAGATGCATCAATACTACGTCCATAATCCATTATTTTTACGCATTATGTTTTCCCTCTGTTCATTAAGGCCTTCGTATAGAATAACTATGAACTTTCATAGTAATGTTTTTATAGCAAATATTGGTAATTAGTTATATTAGTATTGTCTCTTTCACCGAGGTTTGAACCTGAATCCTCTTAACTTATTCAACCCTTGGCTCAAATCAGTTGTGCTACCCAACTTACCAAAACTTTCATATCAATTGGTTGATGTTAGCACAAGAACCATAAGGTCATAATTTGATTTACTTTTTTCATGTTACAGGTGAGCTTAGGTGGGCAAGAAATAATAGAAGGTCGTTTGTTGGCTGCACTAAGAGTACTCCTTGCAAGTGATATGGCAAGTGTACAAAAGCATGACCTCAACACACTTAAGTCTTTAGATGCTGAGGCTCCTCTTGGTGTTGCAAATGACCTTGCTGTATTTCGTACCTTGATTGCTCTGTGTGTCATTGCACTAGGACACTTTGCCACCAAATTAATGGATGATGAATCTTTATTGAAGCAGGGTGCTTCAGGTTCAACAGAATTAGCCATTCAGTTCAGAATCCAAAAGAAGTCTGTAATTATAGATGTAATGAAAAACCTCTCAAGGAAGGTAAAGCCTCTTTCATCAAAGGAGACGGTCACTACTGAAGGCTAA
Protein sequence

>MS.gene015660.t1

MVIVGGGSWKTAMLMSSSLSHVRPLTCASSSSSSSRVAPHPHDLIKWVTREGGFVHRAVKIAQLDSSNGLGLVAKEDIPIGTDLIALPQHIPLHFNDDDRNSHSLLLQLTSHVPDELWAMKLGLKLLHERAKLGSFWWPYISNLPQTYTLPIFFPGEDIKNLHYAPILHQVNKRCRFLLDFEQKVKHALVGLTPDKHPFGGQEVDASSLGWAMSAVSSRAFKLHGNKQSNGINFDIPMMLPLIDMCNHSFNPNARIVQEQEAGSTKMWVKVVAEKAIKEDDPLLLCYGCLSNDLFLLDYGFVIQSNPYDCIELKYDGALLDAASMAAGVSSPNFSTPAPWQELILSQLNLAGETPDLKVSLGGQEIIEGRLLAALRVLLASDMASVQKHDLNTLKSLDAEAPLGVANDLAVFRTLIALCVIALGHFATKLMDDESLLKQGASGSTELAIQFRIQKKSVIIDVMKNLSRKVKPLSSKETVTTEG