AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015731

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015731.t1 MTR_8g103940 90.081 494 47 2 4 497 7 498 0.0 909
MS.gene015731.t1 MTR_1g108780 68.017 469 137 5 30 495 1 459 0.0 649
MS.gene015731.t1 MTR_1g035460 43.704 405 203 12 52 442 2 395 1.54e-95 295
MS.gene015731.t1 MTR_7g050710 37.658 316 158 9 34 329 11 307 3.51e-58 196
MS.gene015731.t1 MTR_3g098790 35.583 326 179 7 34 347 11 317 3.40e-52 180
MS.gene015731.t1 MTR_7g050720 35.647 317 170 10 36 329 10 315 8.66e-49 171
MS.gene015731.t1 MTR_7g050560 37.013 308 154 9 34 329 11 290 9.66e-48 167
MS.gene015731.t1 MTR_7g050580 36.478 318 138 9 36 329 13 290 1.75e-47 167
MS.gene015731.t1 MTR_7g050590 36.610 295 154 10 52 329 2 280 2.14e-47 166
MS.gene015731.t1 MTR_3g061340 34.114 299 151 10 35 325 11 271 1.94e-46 163
MS.gene015731.t1 MTR_3g061340 39.394 99 52 3 399 493 180 274 5.49e-12 66.6
MS.gene015731.t1 MTR_5g044670 31.515 330 190 8 21 335 1 309 2.90e-46 164
MS.gene015731.t1 MTR_3g061370 32.288 319 152 8 32 328 5 281 3.05e-46 162
MS.gene015731.t1 MTR_3g061350 33.754 317 146 10 34 328 10 284 8.90e-44 156
MS.gene015731.t1 MTR_3g061320 33.119 311 151 11 34 328 10 279 7.85e-43 153
MS.gene015731.t1 MTR_3g061320 39.583 96 56 2 399 493 185 279 5.21e-11 63.9
MS.gene015731.t1 MTR_3g061330 33.216 283 139 10 28 295 4 251 2.34e-42 151
MS.gene015731.t1 MTR_3g061310 31.646 316 167 8 28 328 4 285 1.00e-40 148
MS.gene015731.t1 MTR_3g061310 39.706 136 72 5 362 493 156 285 8.51e-17 81.3
MS.gene015731.t1 MTR_1g035570 26.483 472 227 16 47 492 16 393 3.05e-38 144
MS.gene015731.t1 MTR_3g061290 42.331 163 83 3 28 181 4 164 1.74e-34 129
MS.gene015731.t1 MTR_1g035640 27.500 480 282 18 47 492 16 463 9.37e-32 127
MS.gene015731.t1 MTR_4g119990 30.154 325 191 13 33 337 12 320 1.55e-31 125
MS.gene015731.t1 MTR_1g035620 25.309 486 266 17 29 494 2 410 4.69e-31 124
MS.gene015731.t1 MTR_6g061070 24.551 501 318 15 21 493 2 470 1.46e-27 115
MS.gene015731.t1 MTR_7g050640 48.936 94 48 0 36 129 13 106 2.55e-26 103
MS.gene015731.t1 MTR_3g005490 26.885 305 152 7 36 332 21 262 2.95e-26 107
MS.gene015731.t1 MTR_5g070440 28.146 302 155 10 36 329 21 268 2.99e-26 108
MS.gene015731.t1 MTR_1g035490 30.928 291 149 12 216 492 75 327 4.29e-25 106
MS.gene015731.t1 MTR_6g043890 26.780 295 199 8 33 320 12 296 1.89e-24 104
MS.gene015731.t1 MTR_1g034210 24.793 363 199 9 36 332 7 361 5.15e-24 103
MS.gene015731.t1 MTR_3g005480 26.557 305 150 8 36 330 21 261 1.47e-23 100
MS.gene015731.t1 MTR_3g005420 26.246 301 162 6 36 329 289 536 1.87e-23 103
MS.gene015731.t1 MTR_3g005420 31.008 258 149 15 251 497 300 539 7.14e-11 65.1
MS.gene015731.t1 MTR_3g005420 33.721 86 56 1 50 134 54 139 7.80e-11 64.7
MS.gene015731.t1 MTR_1g050845 28.165 316 158 10 29 327 2 265 1.47e-22 102
MS.gene015731.t1 MTR_3g005460 24.522 314 173 8 28 328 254 516 3.29e-22 100
MS.gene015731.t1 MTR_3g005460 35.294 85 55 0 50 134 39 123 1.92e-12 69.7
MS.gene015731.t1 MTR_1g021270 21.403 556 318 21 49 492 28 576 4.90e-22 99.8
MS.gene015731.t1 MTR_4g068320 31.325 166 108 1 36 195 6 171 7.69e-21 95.1
MS.gene015731.t1 MTR_4g068320 33.333 177 106 6 174 339 224 399 2.90e-18 87.4
MS.gene015731.t1 MTR_3g061220 41.121 107 48 2 28 134 4 95 9.91e-21 87.8
MS.gene015731.t1 MTR_3g005495 25.172 290 145 7 36 320 243 465 6.89e-19 89.7
MS.gene015731.t1 MTR_3g005495 34.524 84 55 0 51 134 28 111 9.01e-13 70.9
MS.gene015731.t1 MTR_1g034240 39.000 100 61 0 36 135 7 106 2.00e-17 84.7
MS.gene015731.t1 MTR_1g034240 37.391 115 65 4 227 335 285 398 2.09e-15 78.6
MS.gene015731.t1 MTR_1g021330 25.140 358 216 14 34 346 24 374 5.67e-17 83.2
MS.gene015731.t1 MTR_1g035650 29.143 175 117 3 35 204 8 180 1.86e-16 78.6
MS.gene015731.t1 MTR_1g035520 26.814 317 169 14 52 327 88 382 3.10e-16 80.9
MS.gene015731.t1 MTR_1g021400 22.767 347 205 12 34 335 28 356 7.27e-16 79.7
MS.gene015731.t1 MTR_3g061300 40.952 105 52 4 392 492 24 122 1.97e-15 73.2
MS.gene015731.t1 MTR_1g021435 24.198 343 213 12 34 333 20 358 2.87e-15 77.8
MS.gene015731.t1 MTR_1g021440 22.067 358 229 12 34 346 21 373 8.91e-15 76.3
MS.gene015731.t1 MTR_1g035440 35.345 116 69 3 23 134 1 114 1.61e-14 73.9
MS.gene015731.t1 MTR_1g021360 23.929 280 182 8 50 316 196 457 5.30e-14 74.7
MS.gene015731.t1 MTR_1g034210 22.756 312 173 6 36 286 7 311 8.47e-14 72.8
MS.gene015731.t1 MTR_1g021410 22.619 336 210 10 37 333 23 347 3.63e-13 71.2
MS.gene015731.t1 MTR_1g021380 22.485 338 201 11 34 333 20 334 9.64e-13 69.7
MS.gene015731.t1 MTR_1g035480 30.328 122 81 3 33 153 14 132 1.63e-12 66.6
MS.gene015731.t1 MTR_1g035500 31.148 122 80 3 33 153 22 140 3.79e-12 65.5
MS.gene015731.t1 MTR_3g005440 34.118 85 56 0 50 134 34 118 3.90e-12 68.6
MS.gene015731.t1 MTR_3g005440 28.058 139 95 1 40 178 246 379 6.24e-12 67.8
MS.gene015731.t1 MTR_1g035470 33.058 121 74 5 52 170 2 117 2.05e-11 62.4
MS.gene015731.t1 MTR_3g005420 33.721 86 56 1 50 134 35 120 8.38e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015731.t1 AT3G18990 33.728 338 179 12 36 332 6 339 4.96e-46 164
MS.gene015731.t1 AT3G18990 33.631 336 178 12 36 330 6 337 5.47e-45 162
MS.gene015731.t1 AT3G18960 40.385 104 62 0 35 138 29 132 3.78e-20 89.0
MS.gene015731.t1 AT4G01580 40.385 104 62 0 35 138 29 132 5.03e-20 88.2
MS.gene015731.t1 AT3G18960 40.385 104 62 0 35 138 29 132 5.71e-20 88.6
MS.gene015731.t1 AT1G49480 38.393 112 61 4 227 332 115 224 7.00e-18 83.2
MS.gene015731.t1 AT1G49480 38.393 112 61 4 227 332 115 224 7.00e-18 83.2
MS.gene015731.t1 AT1G49480 38.393 112 61 4 227 332 115 224 7.00e-18 83.2
MS.gene015731.t1 AT1G49475 29.371 143 95 2 10 147 3 144 1.78e-17 80.9
MS.gene015731.t1 AT3G18960 42.045 88 51 0 51 138 8 95 3.59e-16 76.6
MS.gene015731.t1 AT4G33280 26.014 296 190 10 50 329 37 319 3.31e-13 71.2
MS.gene015731.t1 AT4G31640 25.794 252 149 8 253 497 25 245 3.17e-11 65.5

Find 71 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTAAACTTGTCCAAGTTAA+AGG 0.118978 4.3:-1940097 MS.gene015731:intron
GAACAAACTTGTTGAAAATT+TGG 0.135205 4.3:-1940242 MS.gene015731:CDS
CTTGATCATGCTTCACATTT+AGG 0.162093 4.3:+1939944 None:intergenic
TGTCATGACAACAGTGAAAA+AGG 0.224513 4.3:+1939852 None:intergenic
CCTTTAACTTGGACAAGTTT+AGG 0.279816 4.3:+1940097 None:intergenic
TTAATGGAGATGGAATTAAT+TGG 0.309089 4.3:-1939334 MS.gene015731:CDS
GTTTGTAGAGGAAAATAAAT+TGG 0.310756 4.3:-1938526 MS.gene015731:CDS
ATAGCAGAATGCAAGTTTCT+TGG 0.360832 4.3:+1941592 None:intergenic
TACTTTAGTTCAAAATCTAC+TGG 0.361077 4.3:+1939670 None:intergenic
TATTTCTTAGTGAAAGCATT+TGG 0.366998 4.3:+1940517 None:intergenic
CTCAATTTCTTATGTGGTTG+AGG 0.369014 4.3:+1940169 None:intergenic
GGAAGTACATTTGACCAAAA+AGG 0.369637 4.3:-1940440 MS.gene015731:CDS
CTTCACATTTAGGCATTTAG+TGG 0.383770 4.3:+1939954 None:intergenic
ACCTCAACAAATATTCTAGA+TGG 0.390301 4.3:-1941550 MS.gene015731:CDS
CAGAGTGGATGTGAATTGAT+TGG 0.396589 4.3:+1938937 None:intergenic
TAATGGAGATGGAATTAATT+GGG 0.397926 4.3:-1939333 MS.gene015731:CDS
AAAACTATCATGTCACATAA+AGG 0.413575 4.3:-1939278 MS.gene015731:intron
CATATTTAACAAGAGTACTT+TGG 0.415158 4.3:-1940305 MS.gene015731:CDS
ACTCAACTTGGTTGACGGTT+CGG 0.425773 4.3:+1938563 None:intergenic
TCTCATTATCCATGACTTGA+AGG 0.426650 4.3:+1939620 None:intergenic
CTGGAGTCTTGAGAAACATT+GGG 0.426683 4.3:+1940476 None:intergenic
TAGCAGAATGCAAGTTTCTT+GGG 0.429765 4.3:+1941593 None:intergenic
CCAATTCTCAATTTCTTATG+TGG 0.430787 4.3:+1940163 None:intergenic
CTTGTGACACAAGTACTTCT+CGG 0.432076 4.3:+1938647 None:intergenic
AAACCTGTGGCATGTAAGAT+TGG 0.435151 4.3:-1938598 MS.gene015731:CDS
ATATTTGGATTCACAAGGGT+TGG 0.435488 4.3:-1940411 MS.gene015731:CDS
CCACATAAGAAATTGAGAAT+TGG 0.439783 4.3:-1940163 MS.gene015731:CDS
AATTTATTATACCTTTAACT+TGG 0.446187 4.3:+1940086 None:intergenic
AGCGGTACGTACCAGATCAT+TGG 0.447934 4.3:+1941841 None:intergenic
CCTGTACTCAACTTGGTTGA+CGG 0.457593 4.3:+1938558 None:intergenic
GATCTAGTCTTCTTGCATGT+AGG 0.464753 4.3:+1940211 None:intergenic
TTTGATAATAATTTGAAAGT+GGG 0.464811 4.3:-1939538 MS.gene015731:CDS
CTTGGAATGTTAAACATTGT+GGG 0.475665 4.3:-1939594 MS.gene015731:CDS
TTTGAATCAGTTAATGGAGA+TGG 0.481033 4.3:-1939344 MS.gene015731:intron
TTGGCATACCACAGGAAAGA+AGG 0.492460 4.3:-1939023 MS.gene015731:intron
TCATGGATAATGAGAGAACT+TGG 0.492868 4.3:-1939612 MS.gene015731:CDS
ATTTGATAATAATTTGAAAG+TGG 0.495131 4.3:-1939539 MS.gene015731:CDS
TAGAGTTACCTTGCGGTGGA+AGG 0.508812 4.3:+1939434 None:intergenic
TCTGGAGTCTTGAGAAACAT+TGG 0.510455 4.3:+1940475 None:intergenic
ACTTGGAATGTTAAACATTG+TGG 0.512866 4.3:-1939595 MS.gene015731:CDS
TGTGGGAGACATATTAGTAC+TGG 0.514312 4.3:-1939577 MS.gene015731:CDS
AACTTGGTTGACGGTTCGGA+TGG 0.514973 4.3:+1938567 None:intergenic
AATAAAGAGACCCAATGATC+TGG 0.516601 4.3:-1941852 None:intergenic
GCTTTCACTAAGAAATATAG+TGG 0.526593 4.3:-1940511 MS.gene015731:CDS
AGGTAACAATCTTAAAGAAA+CGG 0.529659 4.3:+1941569 None:intergenic
CGTTATCACCCTTCAAGTCA+TGG 0.529744 4.3:-1939629 MS.gene015731:CDS
ACTCCAACCTGTACTCAACT+TGG 0.532809 4.3:+1938551 None:intergenic
TGTAGAGGAAAATAAATTGG+AGG 0.533957 4.3:-1938523 MS.gene015731:CDS
GAGGAAAATAAATTGGAGGA+TGG 0.537388 4.3:-1938519 MS.gene015731:CDS
TCGCCAATCTTACATGCCAC+AGG 0.542280 4.3:+1938595 None:intergenic
CTCATTATCCATGACTTGAA+GGG 0.556492 4.3:+1939621 None:intergenic
AGAGTTACCTTGCGGTGGAA+GGG 0.558988 4.3:+1939435 None:intergenic
TCATGCGGATGTTTACAAAC+CGG 0.561557 4.3:-1938879 MS.gene015731:intron
GAAACATCAGCATGTGTCCA+TGG 0.562484 4.3:+1940359 None:intergenic
TCCATCTAGAATATTTGTTG+AGG 0.564502 4.3:+1941549 None:intergenic
CCGTCAACCAAGTTGAGTAC+AGG 0.579549 4.3:-1938558 MS.gene015731:CDS
AGGTTGGAGTAAGTTTGTAG+AGG 0.581140 4.3:-1938538 MS.gene015731:CDS
TGGTGATATTTGGATTCACA+AGG 0.584168 4.3:-1940416 MS.gene015731:CDS
TTCACTTTGATAGTGAAATG+TGG 0.584688 4.3:+1938908 None:intergenic
ACTTTAGTTCAAAATCTACT+GGG 0.593567 4.3:+1939671 None:intergenic
ATTGGCGCAAGTGAAGATGT+TGG 0.596112 4.3:-1940145 MS.gene015731:CDS
GGAGACATATTAGTACTGGA+TGG 0.606406 4.3:-1939573 MS.gene015731:CDS
TTCGTATGCTAATAAATACA+TGG 0.609845 4.3:-1939827 MS.gene015731:intron
CAACCAAGTTGAGTACAGGT+TGG 0.611957 4.3:-1938554 MS.gene015731:CDS
CAACTAGAGTTACCTTGCGG+TGG 0.614291 4.3:+1939430 None:intergenic
AAACATTCCCTTCCACCGCA+AGG 0.615531 4.3:-1939442 MS.gene015731:intron
ACACATTATTCTATAGACCA+TGG 0.617727 4.3:-1940376 MS.gene015731:CDS
TATGTAAACCTCAAAATGAG+AGG 0.618304 4.3:+1940324 None:intergenic
CAAAGTGAAATCAAGTCATG+CGG 0.640337 4.3:-1938894 MS.gene015731:CDS
AAGTATTTGAAGGAAAAGCA+AGG 0.649480 4.3:-1939652 MS.gene015731:CDS
GGTGATATTTGGATTCACAA+GGG 0.673550 4.3:-1940415 MS.gene015731:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTGTTATAATATTAAAAA+CGG - chr4.3:1939281-1939300 MS.gene015731:CDS 10.0%
!! TCAAAAATCAAAATATTAAT+TGG + chr4.3:1940254-1940273 None:intergenic 10.0%
!!! GATTTTTTTTTTTTAGTATT+TGG + chr4.3:1939327-1939346 None:intergenic 10.0%
!! AATTTATTATACCTTTAACT+TGG + chr4.3:1940198-1940217 None:intergenic 15.0%
!! ACTGTTATAATATTAAAAAC+GGG - chr4.3:1939282-1939301 MS.gene015731:CDS 15.0%
!! TTTAATATTATAACAGTTAG+TGG + chr4.3:1939279-1939298 None:intergenic 15.0%
!!! ATCACTTTTTTAATGTTAAA+TGG - chr4.3:1939347-1939366 MS.gene015731:intron 15.0%
!!! ATTTGATAATAATTTGAAAG+TGG - chr4.3:1940742-1940761 MS.gene015731:intron 15.0%
!!! TAACTAAAATAATTTGTAAG+TGG + chr4.3:1939445-1939464 None:intergenic 15.0%
!!! TTAAGTTTTGATATTTGAAA+AGG + chr4.3:1938901-1938920 None:intergenic 15.0%
!!! TTTAGGAATTTTTTTCAATT+TGG + chr4.3:1940170-1940189 None:intergenic 15.0%
!!! TTTGATAATAATTTGAAAGT+GGG - chr4.3:1940743-1940762 MS.gene015731:intron 15.0%
!! AAACATAATCATGAGATAAA+TGG + chr4.3:1941507-1941526 None:intergenic 20.0%
!! ACTACTCCTTAAAAAAAAAA+TGG + chr4.3:1939074-1939093 None:intergenic 20.0%
!! AGGTATAATAAATTCATGAT+TGG - chr4.3:1940204-1940223 MS.gene015731:CDS 20.0%
!! CTGTTATAATATTAAAAACG+GGG - chr4.3:1939283-1939302 MS.gene015731:CDS 20.0%
!! CTTAAAAAAAAAATGGTTAG+TGG + chr4.3:1939067-1939086 None:intergenic 20.0%
!! GTTGCTTAAAAAAAGTTTAT+TGG - chr4.3:1939391-1939410 MS.gene015731:intron 20.0%
!! TAAAAATTTATAGAGAGAAG+CGG + chr4.3:1938461-1938480 None:intergenic 20.0%
!!! AATTGATTTTTTGCTTTTGT+TGG - chr4.3:1941239-1941258 MS.gene015731:intron 20.0%
!!! ATTTTTGAAGATGGATTATT+CGG + chr4.3:1940365-1940384 None:intergenic 20.0%
!!! CACTAACCATTTTTTTTTTA+AGG - chr4.3:1939065-1939084 MS.gene015731:intron 20.0%
!!! CTTCTCTCTATAAATTTTTA+GGG - chr4.3:1938461-1938480 MS.gene015731:CDS 20.0%
!!! TTTATCTATCTCTATTGTTT+TGG + chr4.3:1939160-1939179 None:intergenic 20.0%
!!! TTTTGAACTAAAGTATTTGA+AGG - chr4.3:1940619-1940638 MS.gene015731:intron 20.0%
! AAAACTATCATGTCACATAA+AGG - chr4.3:1941003-1941022 MS.gene015731:intron 25.0%
! AAATAAAAGTTTAGGAGAGT+TGG + chr4.3:1939643-1939662 None:intergenic 25.0%
! AAGAGAGAAATATACATATG+TGG + chr4.3:1939684-1939703 None:intergenic 25.0%
! AGGTAACAATCTTAAAGAAA+CGG + chr4.3:1938715-1938734 None:intergenic 25.0%
! ATCATGAGATAAATGGTATT+TGG + chr4.3:1941500-1941519 None:intergenic 25.0%
! CAACAGTATTTAGTACAAAA+CGG - chr4.3:1941075-1941094 MS.gene015731:intron 25.0%
! CTCTTCTTCATTCTTTAATT+TGG - chr4.3:1938781-1938800 MS.gene015731:intron 25.0%
! GAAAGAAAGAATTGTTACTA+GGG + chr4.3:1938759-1938778 None:intergenic 25.0%
! GTTTGTAGAGGAAAATAAAT+TGG - chr4.3:1941755-1941774 MS.gene015731:intron 25.0%
! TAATGGAGATGGAATTAATT+GGG - chr4.3:1940948-1940967 MS.gene015731:intron 25.0%
! TACAGTTTAATAAAAACCTG+TGG - chr4.3:1941670-1941689 MS.gene015731:intron 25.0%
! TACTTACACAAAATGTAAGT+AGG - chr4.3:1938818-1938837 MS.gene015731:intron 25.0%
! TCTAAAAATGTCAAATCTTG+GGG - chr4.3:1939588-1939607 MS.gene015731:CDS 25.0%
! TGAAAGAAAGAATTGTTACT+AGG + chr4.3:1938760-1938779 None:intergenic 25.0%
! TTAATGGAGATGGAATTAAT+TGG - chr4.3:1940947-1940966 MS.gene015731:intron 25.0%
! TTCGTATGCTAATAAATACA+TGG - chr4.3:1940454-1940473 MS.gene015731:CDS 25.0%
! TTTGTTGATCTCAAGAATAA+TGG - chr4.3:1940899-1940918 MS.gene015731:intron 25.0%
!! ACTTTAGTTCAAAATCTACT+GGG + chr4.3:1940613-1940632 None:intergenic 25.0%
!! CTCTAAAAATGTCAAATCTT+GGG - chr4.3:1939587-1939606 MS.gene015731:CDS 25.0%
!! GCTTCTCTCTATAAATTTTT+AGG - chr4.3:1938460-1938479 MS.gene015731:CDS 25.0%
!! TACTTTAGTTCAAAATCTAC+TGG + chr4.3:1940614-1940633 None:intergenic 25.0%
!! TCTCTAAAAATGTCAAATCT+TGG - chr4.3:1939586-1939605 MS.gene015731:CDS 25.0%
!! TGCTTATATTTAAGATCAGA+GGG + chr4.3:1941447-1941466 None:intergenic 25.0%
!! TTGCTTATATTTAAGATCAG+AGG + chr4.3:1941448-1941467 None:intergenic 25.0%
!!! AAAGTACTAATTTTTCGACT+AGG - chr4.3:1940812-1940831 MS.gene015731:intron 25.0%
!!! ATAGTTTTATGTTCAACTCT+AGG + chr4.3:1940991-1941010 None:intergenic 25.0%
!!! CATATTTAACAAGAGTACTT+TGG - chr4.3:1939976-1939995 MS.gene015731:intron 25.0%
!!! GAACAAACTTGTTGAAAATT+TGG - chr4.3:1940039-1940058 MS.gene015731:intron 25.0%
!!! TAGTTTTATGTTCAACTCTA+GGG + chr4.3:1940990-1941009 None:intergenic 25.0%
!!! TATTTCTTAGTGAAAGCATT+TGG + chr4.3:1939767-1939786 None:intergenic 25.0%
!!! TTGTCTAGACTTTAAGATTA+GGG - chr4.3:1938854-1938873 MS.gene015731:intron 25.0%
!!! TTTGTCTAGACTTTAAGATT+AGG - chr4.3:1938853-1938872 MS.gene015731:intron 25.0%
AAGAGAAAGTCGTCATAAAA+TGG - chr4.3:1939203-1939222 MS.gene015731:intron 30.0%
AAGTATTTGAAGGAAAAGCA+AGG - chr4.3:1940629-1940648 MS.gene015731:intron 30.0%
ACACATTATTCTATAGACCA+TGG - chr4.3:1939905-1939924 MS.gene015731:CDS 30.0%
ACCTCAACAAATATTCTAGA+TGG - chr4.3:1938731-1938750 MS.gene015731:intron 30.0%
ACTTGGAATGTTAAACATTG+TGG - chr4.3:1940686-1940705 MS.gene015731:intron 30.0%
ATCATATTACCCTATTACCA+AGG + chr4.3:1939555-1939574 None:intergenic 30.0%
CCAATTCTCAATTTCTTATG+TGG + chr4.3:1940121-1940140 None:intergenic 30.0%
CCACATAAGAAATTGAGAAT+TGG - chr4.3:1940118-1940137 MS.gene015731:CDS 30.0%
CTTGGAATGTTAAACATTGT+GGG - chr4.3:1940687-1940706 MS.gene015731:intron 30.0%
GCTTTCACTAAGAAATATAG+TGG - chr4.3:1939770-1939789 MS.gene015731:intron 30.0%
TAATTCATGTCATGCATTCT+TGG - chr4.3:1941544-1941563 MS.gene015731:CDS 30.0%
TATGTAAACCTCAAAATGAG+AGG + chr4.3:1939960-1939979 None:intergenic 30.0%
TGTAGAGGAAAATAAATTGG+AGG - chr4.3:1941758-1941777 MS.gene015731:intron 30.0%
TTTGAATCAGTTAATGGAGA+TGG - chr4.3:1940937-1940956 MS.gene015731:intron 30.0%
! AACATTGTTGTGACTTCTTT+TGG - chr4.3:1941035-1941054 MS.gene015731:intron 30.0%
! ATCTCCATTAACTGATTCAA+AGG + chr4.3:1940938-1940957 None:intergenic 30.0%
! CGTTTTGTACTAAATACTGT+TGG + chr4.3:1941077-1941096 None:intergenic 30.0%
! CTCATGATTATGTTTGAAAC+TGG - chr4.3:1941512-1941531 MS.gene015731:intron 30.0%
! TCCATCTAGAATATTTGTTG+AGG + chr4.3:1938735-1938754 None:intergenic 30.0%
! TGAAAAAACCTCTCATTTTG+AGG - chr4.3:1939949-1939968 MS.gene015731:CDS 30.0%
! TTTGATGCTCATATTTTCAG+AGG - chr4.3:1941828-1941847 MS.gene015731:intron 30.0%
!! TTCACTTTGATAGTGAAATG+TGG + chr4.3:1941376-1941395 None:intergenic 30.0%
!!! ATTTGGTGTTCCTTTTGATT+AGG + chr4.3:1939310-1939329 None:intergenic 30.0%
AATCAACTAGAGTTACCTTG+CGG + chr4.3:1940857-1940876 None:intergenic 35.0%
AGACTCCAGATGACAAAAAA+TGG - chr4.3:1939819-1939838 MS.gene015731:intron 35.0%
ATAGCAGAATGCAAGTTTCT+TGG + chr4.3:1938692-1938711 None:intergenic 35.0%
ATATTTGGATTCACAAGGGT+TGG - chr4.3:1939870-1939889 MS.gene015731:CDS 35.0%
CAAAGTGAAATCAAGTCATG+CGG - chr4.3:1941387-1941406 MS.gene015731:intron 35.0%
CCAAAAAGGATGGTGATATT+TGG - chr4.3:1939855-1939874 MS.gene015731:CDS 35.0%
CCTAAACTTGTCCAAGTTAA+AGG - chr4.3:1940184-1940203 MS.gene015731:CDS 35.0%
CTCAATTTCTTATGTGGTTG+AGG + chr4.3:1940115-1940134 None:intergenic 35.0%
CTCATTATCCATGACTTGAA+GGG + chr4.3:1940663-1940682 None:intergenic 35.0%
CTTCACATTTAGGCATTTAG+TGG + chr4.3:1940330-1940349 None:intergenic 35.0%
CTTGATCATGCTTCACATTT+AGG + chr4.3:1940340-1940359 None:intergenic 35.0%
GACTCCAGATGACAAAAAAT+GGG - chr4.3:1939820-1939839 MS.gene015731:intron 35.0%
GAGGAAAATAAATTGGAGGA+TGG - chr4.3:1941762-1941781 MS.gene015731:intron 35.0%
GGTGATATTTGGATTCACAA+GGG - chr4.3:1939866-1939885 MS.gene015731:CDS 35.0%
GTACATTTGACCAAAAAGGA+TGG - chr4.3:1939845-1939864 MS.gene015731:CDS 35.0%
GTCATGACAACAGTGAAAAA+GGG + chr4.3:1940431-1940450 None:intergenic 35.0%
TAGCAGAATGCAAGTTTCTT+GGG + chr4.3:1938691-1938710 None:intergenic 35.0%
TAGGGTCGGTCTAATTAAAT+CGG - chr4.3:1938872-1938891 MS.gene015731:intron 35.0%
TCATGGATAATGAGAGAACT+TGG - chr4.3:1940669-1940688 MS.gene015731:intron 35.0%
TCGTCCTTTGAATCAGTTAA+TGG - chr4.3:1940931-1940950 MS.gene015731:intron 35.0%
TCTCATTATCCATGACTTGA+AGG + chr4.3:1940664-1940683 None:intergenic 35.0%
TGGTGATATTTGGATTCACA+AGG - chr4.3:1939865-1939884 MS.gene015731:CDS 35.0%
TGTCATGACAACAGTGAAAA+AGG + chr4.3:1940432-1940451 None:intergenic 35.0%
! CCAAATATCACCATCCTTTT+TGG + chr4.3:1939858-1939877 None:intergenic 35.0%
! GGAAGTACATTTGACCAAAA+AGG - chr4.3:1939841-1939860 MS.gene015731:CDS 35.0%
!! CCTTTAACTTGGACAAGTTT+AGG + chr4.3:1940187-1940206 None:intergenic 35.0%
!! CTAGACTTTAAGATTAGGGT+CGG - chr4.3:1938858-1938877 MS.gene015731:intron 35.0%
!! GAAATGTGGATTTTTCAGAG+TGG + chr4.3:1941362-1941381 None:intergenic 35.0%
!! GCTTCAGCAATTTTTGAAGA+TGG + chr4.3:1940374-1940393 None:intergenic 35.0%
!! TGTTTTTGAGCTCGTCAATA+AGG - chr4.3:1941794-1941813 MS.gene015731:intron 35.0%
!! TTTGGAATTTTCTGCAGTCA+TGG + chr4.3:1939749-1939768 None:intergenic 35.0%
!!! ACTTCCCATTTTTTGTCATC+TGG + chr4.3:1939827-1939846 None:intergenic 35.0%
AAAACGGGGTCCTAATCAAA+AGG - chr4.3:1939297-1939316 MS.gene015731:CDS 40.0%
AAACCTGTGGCATGTAAGAT+TGG - chr4.3:1941683-1941702 MS.gene015731:intron 40.0%
AGAGTGTGTGTATTGCTTAC+CGG + chr4.3:1941424-1941443 None:intergenic 40.0%
AGGTTGGAGTAAGTTTGTAG+AGG - chr4.3:1941743-1941762 MS.gene015731:intron 40.0%
CAAAATCTACTGGGATGTAC+TGG + chr4.3:1940604-1940623 None:intergenic 40.0%
CAGAGTGGATGTGAATTGAT+TGG + chr4.3:1941347-1941366 None:intergenic 40.0%
CCGAGATTCGCAAAAAATGA+CGG + chr4.3:1941151-1941170 None:intergenic 40.0%
CTGGAGTCTTGAGAAACATT+GGG + chr4.3:1939808-1939827 None:intergenic 40.0%
CTTGTGACACAAGTACTTCT+CGG + chr4.3:1941637-1941656 None:intergenic 40.0%
TAGTGGAGCTCAACAAAAGT+TGG + chr4.3:1939050-1939069 None:intergenic 40.0%
TCATGCGGATGTTTACAAAC+CGG - chr4.3:1941402-1941421 MS.gene015731:intron 40.0%
TGCCATGTTCAATTTCTCAG+AGG - chr4.3:1940292-1940311 MS.gene015731:CDS 40.0%
TGTGGGAGACATATTAGTAC+TGG - chr4.3:1940704-1940723 MS.gene015731:intron 40.0%
TGTGTATACGCTGCAGTTAA+CGG + chr4.3:1941605-1941624 None:intergenic 40.0%
! AGTGTGAGAACCTTGGTAAT+AGG - chr4.3:1939542-1939561 MS.gene015731:CDS 40.0%
! CTCCTCTGAGAAATTGAACA+TGG + chr4.3:1940297-1940316 None:intergenic 40.0%
! GATCTAGTCTTCTTGCATGT+AGG + chr4.3:1940073-1940092 None:intergenic 40.0%
! GGAGACATATTAGTACTGGA+TGG - chr4.3:1940708-1940727 MS.gene015731:intron 40.0%
! GTGTGAGAACCTTGGTAATA+GGG - chr4.3:1939543-1939562 MS.gene015731:CDS 40.0%
! TCTGGAGTCTTGAGAAACAT+TGG + chr4.3:1939809-1939828 None:intergenic 40.0%
! TTTAGGAGAGTTGGTTCCTA+AGG + chr4.3:1939634-1939653 None:intergenic 40.0%
!! CAGTTAGTGGTTAGTTTGAC+TGG + chr4.3:1939266-1939285 None:intergenic 40.0%
!!! CCGTCATTTTTTGCGAATCT+CGG - chr4.3:1941148-1941167 MS.gene015731:intron 40.0%
AACTGTCAGTGTGAGAACCT+TGG - chr4.3:1939535-1939554 MS.gene015731:CDS 45.0%
AAGGTTCTCACACTGACAGT+TGG + chr4.3:1939536-1939555 None:intergenic 45.0%
ACTCAACTTGGTTGACGGTT+CGG + chr4.3:1941721-1941740 None:intergenic 45.0%
ACTCCAACCTGTACTCAACT+TGG + chr4.3:1941733-1941752 None:intergenic 45.0%
ATTATGTTGTCGTAGCGCCA+TGG + chr4.3:1941119-1941138 None:intergenic 45.0%
ATTGGCGCAAGTGAAGATGT+TGG - chr4.3:1940136-1940155 MS.gene015731:CDS 45.0%
CCTGTACTCAACTTGGTTGA+CGG + chr4.3:1941726-1941745 None:intergenic 45.0%
CGATCACCTTTCCTGTGAAT+GGG + chr4.3:1938611-1938630 None:intergenic 45.0%
CGTTATCACCCTTCAAGTCA+TGG - chr4.3:1940652-1940671 MS.gene015731:intron 45.0%
GAAACATCAGCATGTGTCCA+TGG + chr4.3:1939925-1939944 None:intergenic 45.0%
GTTCAGTAGCAGCTATACCA+TGG - chr4.3:1941099-1941118 MS.gene015731:intron 45.0%
TCGATCACCTTTCCTGTGAA+TGG + chr4.3:1938612-1938631 None:intergenic 45.0%
TTGGCATACCACAGGAAAGA+AGG - chr4.3:1941258-1941277 MS.gene015731:intron 45.0%
! CAACCAAGTTGAGTACAGGT+TGG - chr4.3:1941727-1941746 MS.gene015731:intron 45.0%
! GTCAAATCTTGGGGCTTTTC+TGG - chr4.3:1939597-1939616 MS.gene015731:CDS 45.0%
! TGCTTTTGTTGGCATACCAC+AGG - chr4.3:1941250-1941269 MS.gene015731:intron 45.0%
! TGTGAATGGGAGGCATAAGT+CGG + chr4.3:1938598-1938617 None:intergenic 45.0%
! TTGAGTTGCCTTCTTTCCTG+TGG + chr4.3:1941269-1941288 None:intergenic 45.0%
!!! TTTTTTATAAATAAAAGTTT+AGG + chr4.3:1939651-1939670 None:intergenic 5.0%
AAACATTCCCTTCCACCGCA+AGG - chr4.3:1940839-1940858 MS.gene015731:intron 50.0%
AGAGTTACCTTGCGGTGGAA+GGG + chr4.3:1940849-1940868 None:intergenic 50.0%
ATGCCTCCCATTCACAGGAA+AGG - chr4.3:1938602-1938621 MS.gene015731:CDS 50.0%
CAACTAGAGTTACCTTGCGG+TGG + chr4.3:1940854-1940873 None:intergenic 50.0%
GACTTATGCCTCCCATTCAC+AGG - chr4.3:1938597-1938616 MS.gene015731:CDS 50.0%
TAGAGTTACCTTGCGGTGGA+AGG + chr4.3:1940850-1940869 None:intergenic 50.0%
TCACCTTTCCTGTGAATGGG+AGG + chr4.3:1938608-1938627 None:intergenic 50.0%
TCGCCAATCTTACATGCCAC+AGG + chr4.3:1941689-1941708 None:intergenic 50.0%
! AACTTGGTTGACGGTTCGGA+TGG + chr4.3:1941717-1941736 None:intergenic 50.0%
! CCGTCAACCAAGTTGAGTAC+AGG - chr4.3:1941723-1941742 MS.gene015731:intron 50.0%
TCTGGACTCTCGCGAGCCTT+AGG - chr4.3:1939615-1939634 MS.gene015731:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 1938443 1941860 1938443 ID=MS.gene015731
chr4.3 mRNA 1938443 1941860 1938443 ID=MS.gene015731.t1;Parent=MS.gene015731
chr4.3 exon 1941853 1941860 1941853 ID=MS.gene015731.t1.exon1;Parent=MS.gene015731.t1
chr4.3 CDS 1941853 1941860 1941853 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1941543 1941684 1941543 ID=MS.gene015731.t1.exon2;Parent=MS.gene015731.t1
chr4.3 CDS 1941543 1941684 1941543 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1940098 1940545 1940098 ID=MS.gene015731.t1.exon3;Parent=MS.gene015731.t1
chr4.3 CDS 1940098 1940545 1940098 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1939828 1939991 1939828 ID=MS.gene015731.t1.exon4;Parent=MS.gene015731.t1
chr4.3 CDS 1939828 1939991 1939828 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1939443 1939698 1939443 ID=MS.gene015731.t1.exon5;Parent=MS.gene015731.t1
chr4.3 CDS 1939443 1939698 1939443 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1939279 1939356 1939279 ID=MS.gene015731.t1.exon6;Parent=MS.gene015731.t1
chr4.3 CDS 1939279 1939356 1939279 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1938880 1939031 1938880 ID=MS.gene015731.t1.exon7;Parent=MS.gene015731.t1
chr4.3 CDS 1938880 1939031 1938880 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
chr4.3 exon 1938443 1938688 1938443 ID=MS.gene015731.t1.exon8;Parent=MS.gene015731.t1
chr4.3 CDS 1938443 1938688 1938443 ID=cds.MS.gene015731.t1;Parent=MS.gene015731.t1
Gene Sequence

>MS.gene015731

ATGATCTGGTACGTACCGCTTCTCTCTATAAATTTTTAGGGTTGTTTTTTGTGTTTTGTTTATTTACTGTTCATCGATATATGCTGTTGCGTGTGAATTTTACAAACTTAGATTTTTTTAATCAAGTAGAGTATGTAGTCAATGATCATTAACCGACTTATGCCTCCCATTCACAGGAAAGGTGATCGATCGCTAAACATTAATACAAATCATCACAATTCTGAAGATTTATCAATGACTTGCAACCCAAGAAACTTGCATTCTGCTATCCGTTTCTTTAAGATTGTTACCTCAACAAATATTCTAGATGGAACCCTAGTAACAATTCTTTCTTTCATCTCTTCTTCATTCTTTAATTTGGATTTTTTTTTTTTTTACTTACACAAAATGTAAGTAGGTATTGTTTGTTGTTTGTCTAGACTTTAAGATTAGGGTCGGTCTAATTAAATCGGAGACCTTTTCAAATATCAAAACTTAACTCTTAATAAAAAAAATGCATTTTTTTTAAAGAGTCATCATCTATTTCAATTAAAAATAACTTTAATAACATAAAAAAGTAGACATCTTTATAAATTAAAATAAAAGTAAGTTTTTACGAACGTAACCAACTTTTGTTGAGCTCCACTAACCATTTTTTTTTTAAGGAGTAGTGTAATTTGACAACTTATGAGACAACTATAATTTACAAAGAAAAAGTGTGTATTTGCACGAAAACCAAAACAATAGAGATAGATAAAGTAAAAAAATAATGTGAGTATGAAAGAGAAAGTCGTCATAAAATGGATGTTCAAATATCATTTCTCTTTTTTAAGAAGACAAACCAGTCAAACTAACCACTAACTGTTATAATATTAAAAACGGGGTCCTAATCAAAAGGAACACCAAATACTAAAAAAAAAAAATCATCACTTTTTTAATGTTAAATGGATCTTAAGTATATTCGTCTTTGTTGCTTAAAAAAAGTTTATTGGTCTTTGTTGATTAACAAACAAATTATAACCACTTACAAATTATTTTAGTTAAATATAAAATTAAATTATTCATATTTCTTAATTTGACTTAACAAGTTTTCAGATGTATATATTTGAATCCAACTGTCAGTGTGAGAACCTTGGTAATAGGGTAATATGATAAAACACTATATCTCTAAAAATGTCAAATCTTGGGGCTTTTCTGGACTCTCGCGAGCCTTAGGAACCAACTCTCCTAAACTTTTATTTATAAAAAAAATACATAAACCACATATGTATATTTCTCTCTTTTGCTTGTGAACTTCATAGTACATGCATGCTAATATAATTTGCCATGACTGCAGAAAATTCCAAATGCTTTCACTAAGAAATATAGTGGTGATTTGTCAAACCCAATGTTTCTCAAGACTCCAGATGACAAAAAATGGGAAGTACATTTGACCAAAAAGGATGGTGATATTTGGATTCACAAGGGTTGGAATGAATTTGCTACACATTATTCTATAGACCATGGACACATGCTGATGTTTCAATATGAAAAAACCTCTCATTTTGAGGTTTACATATTTAACAAGAGTACTTTGGAAATTGAATATCACGTTGATAGTAATAATCAACATGAACAGAACAAACTTGTTGAAAATTTGGATGAAAAACCTACATGCAAGAAGACTAGATCAAAATCACAAATTTCAAGTCCTCAACCACATAAGAAATTGAGAATTGGCGCAAGTGAAGATGTTGGAACAAGCTCCAAATTGAAAAAAATTCCTAAACTTGTCCAAGTTAAAGGTATAATAAATTCATGATTGGATTAATCTCTATCATTTCATTTATCCAATTAATATTTTGATTTTTGAGATTGATTTTTCATAACATGCCATGTTCAATTTCTCAGAGGAGATTGAAGATACCACTAAATGCCTAAATGTGAAGCATGATCAAGAGCCGAATAATCCATCTTCAAAAATTGCTGAAGCTTTAAACAAAGCAAAAAACTACAAAACTAACAATCCCTTTTTCACTGTTGTCATGACATATTCGTATGCTAATAAATACATGGTGAGTCTTTGAATAGCTTCATTTTTTATTTATTTTTATGTTAAGATAGTTTTATATTTTTAACTTCAAGTTAATGTACATCTTAATTATTTGTTAGATTTTCTAACTTGTTACAAAATTTAAATCCAGTACATCCCAGTAGATTTTGAACTAAAGTATTTGAAGGAAAAGCAAGGCGTTATCACCCTTCAAGTCATGGATAATGAGAGAACTTGGAATGTTAAACATTGTGGGAGACATATTAGTACTGGATGGAAAAATTTTGCATTTGATAATAATTTGAAAGTGGGAGATGTTTGTCTTTTTGAGATGATCAAAAGCAATTCTTATGCTTTCAAAGTACTAATTTTTCGACTAGGCGAAAAACATTCCCTTCCACCGCAAGGTAACTCTAGTTGATTTTTCTTTAAGTTATTACTCTTTTTTGTTGATCTCAAGAATAATGGTTATAATTTTCGTCCTTTGAATCAGTTAATGGAGATGGAATTAATTGGGTTGAAACAGCAAGAATCCCTAGAGTTGAACATAAAACTATCATGTCACATAAAGGTAAATCTTTAACATTGTTGTGACTTCTTTTGGAAAGTTAAAGTTGAATCCAACAGTATTTAGTACAAAACGGTGTTCAGTAGCAGCTATACCATGGCGCTACGACAACATAATTCGAATATACCGTCATTTTTTGCGAATCTCGGTTGACAATACTAGAATAAGAAAGCAATGCAACTATGCATATTTCAAAAATAGATGTAGCTTATTTGTAAATTGATTTTTTGCTTTTGTTGGCATACCACAGGAAAGAAGGCAACTCAAAATTCACTGAATGCTAGTTCACGCTCTTTCAAAAGTTTTGAAGTTATGAAAGAATCCAATCAATTCACATCCACTCTGAAAAATCCACATTTCACTATCAAAGTGAAATCAAGTCATGCGGATGTTTACAAACCGGTAAGCAATACACACACTCTCCCTCTGATCTTAAATATAAGCAAAAAAATATATGTATTTGTCGAAATTTGAACCAAATACCATTTATCTCATGATTATGTTTGAAACTGGAGTAGTATTTAATTCATGTCATGCATTCTTGGAGCAAGCATATGCAGTTATAATTATTGACATACTTCCGTTAACTGCAGCGTATACACAACTCGTTCTCCGAGAAGTACTTGTGTCACAAGAAGATTGTAACGTTACAGTTTAATAAAAACCTGTGGCATGTAAGATTGGCGAGCTGTCCATCCGAACCGTCAACCAAGTTGAGTACAGGTTGGAGTAAGTTTGTAGAGGAAAATAAATTGGAGGATGGAGATTCATGTGTTTTTGAGCTCGTCAATAAGGAAGATCTTGTGTTTGATGCTCATATTTTCAGAGGCTGCAATTAG
Protein sequence

>MS.gene015731.t1

MIWKGDRSLNINTNHHNSEDLSMTCNPRNLHSAIRFFKIVTSTNILDGTLKIPNAFTKKYSGDLSNPMFLKTPDDKKWEVHLTKKDGDIWIHKGWNEFATHYSIDHGHMLMFQYEKTSHFEVYIFNKSTLEIEYHVDSNNQHEQNKLVENLDEKPTCKKTRSKSQISSPQPHKKLRIGASEDVGTSSKLKKIPKLVQVKEEIEDTTKCLNVKHDQEPNNPSSKIAEALNKAKNYKTNNPFFTVVMTYSYANKYMYIPVDFELKYLKEKQGVITLQVMDNERTWNVKHCGRHISTGWKNFAFDNNLKVGDVCLFEMIKSNSYAFKVLIFRLGEKHSLPPQVNGDGINWVETARIPRVEHKTIMSHKGKKATQNSLNASSRSFKSFEVMKESNQFTSTLKNPHFTIKVKSSHADVYKPRIHNSFSEKYLCHKKIVTLQFNKNLWHVRLASCPSEPSTKLSTGWSKFVEENKLEDGDSCVFELVNKEDLVFDAHIFRGCN