AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015851

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015851.t1 MTR_8g102100 94.271 384 14 3 1 379 1 381 0.0 718
MS.gene015851.t1 MTR_6g090380 55.610 410 111 8 1 373 1 376 1.39e-142 410
MS.gene015851.t1 MTR_3g103960 44.242 330 114 9 46 374 18 278 8.33e-77 238
MS.gene015851.t1 MTR_4g078660 71.818 110 31 0 75 184 54 163 8.56e-58 191
MS.gene015851.t1 MTR_2g090605 74.757 103 26 0 81 183 77 179 6.11e-57 191
MS.gene015851.t1 MTR_7g092070 72.897 107 29 0 77 183 260 366 5.17e-54 190
MS.gene015851.t1 MTR_5g071070 46.025 239 97 9 77 283 144 382 3.05e-53 186
MS.gene015851.t1 MTR_5g071070 46.025 239 97 9 77 283 226 464 1.12e-52 186
MS.gene015851.t1 MTR_3g029590 51.515 165 70 2 77 235 260 420 1.98e-51 183
MS.gene015851.t1 MTR_5g082830 54.037 161 70 1 77 237 213 369 1.64e-50 180
MS.gene015851.t1 MTR_3g435680 66.379 116 38 1 68 183 139 253 1.95e-50 174
MS.gene015851.t1 MTR_1g050438 63.551 107 39 0 77 183 224 330 1.13e-48 173
MS.gene015851.t1 MTR_0728s0020 63.551 107 39 0 77 183 240 346 1.86e-48 173
MS.gene015851.t1 MTR_1g075890 67.290 107 35 0 77 183 225 331 3.20e-47 170
MS.gene015851.t1 MTR_3g070880 49.693 163 54 3 77 237 159 295 3.70e-47 168
MS.gene015851.t1 MTR_3g435590 47.024 168 78 4 77 243 234 391 8.71e-47 166
MS.gene015851.t1 MTR_3g464270 46.914 162 75 4 79 238 7 159 4.13e-40 145
MS.gene015851.t1 MTR_3g464260 68.056 72 23 0 112 183 212 283 1.10e-29 120
MS.gene015851.t1 MTR_3g464290 60.759 79 30 1 77 154 96 174 4.74e-29 117
MS.gene015851.t1 MTR_3g464280 42.991 107 27 1 77 183 184 256 1.10e-20 94.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015851.t1 AT4G29190 54.569 383 136 14 1 373 1 355 4.33e-127 370
MS.gene015851.t1 AT2G19810 51.571 382 152 10 1 373 1 358 4.74e-115 339
MS.gene015851.t1 AT2G25900 44.904 363 138 9 1 350 1 314 2.03e-90 275
MS.gene015851.t1 AT2G25900 44.904 363 138 9 1 350 1 314 2.03e-90 275
MS.gene015851.t1 AT1G03790 51.724 174 83 1 12 184 13 186 2.84e-58 194
MS.gene015851.t1 AT5G44260 68.548 124 30 2 62 184 49 164 2.34e-55 186
MS.gene015851.t1 AT2G41900 71.028 107 31 0 77 183 257 363 3.24e-53 187
MS.gene015851.t1 AT5G12850 71.963 107 30 0 77 183 253 359 4.95e-53 187
MS.gene015851.t1 AT3G55980 46.829 205 94 4 77 278 207 399 1.45e-51 181
MS.gene015851.t1 AT3G55980 46.829 205 94 4 77 278 274 466 3.40e-51 181
MS.gene015851.t1 AT5G58620 69.159 107 33 0 77 183 205 311 7.50e-51 179
MS.gene015851.t1 AT5G07500 63.333 120 42 1 66 183 35 154 8.97e-51 170
MS.gene015851.t1 AT2G40140 53.459 159 69 1 77 235 210 363 1.16e-48 174
MS.gene015851.t1 AT2G40140 53.459 159 69 1 77 235 210 363 1.16e-48 174

Find 125 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGATCGGGTTCGGGTTC+GGG 0.198833 4.3:+3354818 MS.gene015851:CDS
TAATGGATCGGGTTCGGGTT+CGG 0.208577 4.3:+3354817 MS.gene015851:CDS
GGAGCTGGTGAGCCCTTTCT+TGG 0.256293 4.3:+3354877 MS.gene015851:CDS
GGGTCGGATCACGGGTCCTC+TGG 0.290200 4.3:-3354624 None:intergenic
CGTTGGTAGGCTGCAGAATC+CGG 0.305303 4.3:-3354645 None:intergenic
TCCGGATGTTGGGTGGGTTT+CGG 0.313054 4.3:+3354856 MS.gene015851:CDS
CAACTTCCTTTACGAAAATC+AGG 0.327092 4.3:-3354149 None:intergenic
CTCACACGGCGGAGCAACTT+AGG 0.327174 4.3:+3354294 MS.gene015851:CDS
CACACCGCCGCCATGATGTT+AGG 0.327724 4.3:+3353756 None:intergenic
TGAAGTCATTACCTTCTTCA+TGG 0.329074 4.3:+3354567 MS.gene015851:CDS
TTCTCGCCGGGATGAGAAAA+AGG 0.329763 4.3:-3354077 None:intergenic
CCGGAGTAGTTATACTTTCT+CGG 0.332203 4.3:-3354116 None:intergenic
TGGAATCTGGAAGAGATATA+AGG 0.338125 4.3:+3354756 MS.gene015851:CDS
TGTTGGGTGGGTTTCGGAGC+TGG 0.339180 4.3:+3354862 MS.gene015851:CDS
CGCATGGTGTGTTCGAGTGC+TGG 0.340661 4.3:+3354198 MS.gene015851:CDS
TAATCGTCGTTGGAGGTTAA+AGG 0.353804 4.3:-3353861 None:intergenic
GCCGTTGGATTGTTCATTGT+TGG 0.354051 4.3:-3353825 None:intergenic
GACCGAGTCATCGGAGTAAT+CGG 0.354759 4.3:-3354470 None:intergenic
CAAATGGGTTCTCCTAGATT+CGG 0.355364 4.3:+3354596 MS.gene015851:CDS
TTCTTCATGGAATGTTCAAA+TGG 0.360051 4.3:+3354580 MS.gene015851:CDS
TACTCCGATGACTCGGTCCT+TGG 0.371166 4.3:+3354475 MS.gene015851:CDS
CGTATGACGGTTCTCCTCTC+AGG 0.385313 4.3:+3354360 MS.gene015851:CDS
CGTAAAGGAAGTTGCAAGAA+AGG 0.403608 4.3:+3354158 MS.gene015851:CDS
ACAAACTGGCCGGCGACAAC+TGG 0.411554 4.3:-3354264 None:intergenic
GTGATGGAGAGGGTGGAATC+TGG 0.428735 4.3:+3354743 MS.gene015851:CDS
TGAAGAAGGTAATGACTTCA+TGG 0.429121 4.3:-3354564 None:intergenic
TCCTCCTGGATCGGTTTCTC+CGG 0.431746 4.3:+3354427 MS.gene015851:CDS
GCAGAATCCGGGTCGGATCA+CGG 0.436306 4.3:-3354633 None:intergenic
GAGCTGGTGAGCCCTTTCTT+GGG 0.442916 4.3:+3354878 MS.gene015851:CDS
ACACCGCCGCCATGATGTTA+GGG 0.445475 4.3:+3353757 None:intergenic
CGGAGTAGTTATACTTTCTC+GGG 0.448222 4.3:-3354115 None:intergenic
CCTCTGGGAGACCCGAATCT+AGG 0.450232 4.3:-3354608 None:intergenic
CAGAATCCGGGTCGGATCAC+GGG 0.455393 4.3:-3354632 None:intergenic
GGGTGAAGTGGTTGAAGATC+CGG 0.455522 4.3:+3354838 MS.gene015851:CDS
AACATTCAATCACCCAAGAA+AGG 0.463244 4.3:-3354890 None:intergenic
CCTGCGTAGGTGTCGTTGGT+AGG 0.465199 4.3:-3354658 None:intergenic
TTCGGCGATGCGCACGTGGC+AGG 0.465958 4.3:+3354033 MS.gene015851:CDS
CTTCCGTTCATGTCTCCTCC+TGG 0.466787 4.3:+3354413 MS.gene015851:CDS
TCCGAAACCCACCCAACATC+CGG 0.467976 4.3:-3354857 None:intergenic
ATCAACGCAGTGCACACTCC+GGG 0.469752 4.3:-3354336 None:intergenic
GTTCATGTCTCCTCCTGGAT+CGG 0.471938 4.3:+3354418 MS.gene015851:CDS
GGAATCTGGAAGAGATATAA+GGG 0.473052 4.3:+3354757 MS.gene015851:CDS
TCACACGGCGGAGCAACTTA+GGG 0.475617 4.3:+3354295 MS.gene015851:CDS
ACTCCGATGACTCGGTCCTT+GGG 0.476813 4.3:+3354476 MS.gene015851:CDS
ATTTGAACATTCCATGAAGA+AGG 0.481692 4.3:-3354578 None:intergenic
CGGCGGCGAGCTTTCTCGCC+GGG 0.483303 4.3:-3354089 None:intergenic
CGCAGGTGCCGAGTCGTGGT+AGG 0.483561 4.3:+3354675 MS.gene015851:CDS
GGTCGGATCACGGGTCCTCT+GGG 0.485156 4.3:-3354623 None:intergenic
CGTCTTTACACGGCTGAGTA+CGG 0.485485 4.3:-3354238 None:intergenic
GGATTGTTCATTGTTGGCCA+TGG 0.498597 4.3:-3353819 None:intergenic
TCTTCATGGAATGTTCAAAT+GGG 0.500081 4.3:+3354581 MS.gene015851:CDS
CTCCGACTCAACAGAGTCCC+CGG 0.512371 4.3:+3354318 MS.gene015851:CDS
TCGGTCCTTGGGAAGATCTG+TGG 0.513782 4.3:+3354487 MS.gene015851:CDS
TCCACCGGAGAAACCGATCC+AGG 0.514556 4.3:-3354431 None:intergenic
TGGTTGAAGATCCGGATGTT+GGG 0.514839 4.3:+3354846 MS.gene015851:CDS
GTTGGTAGGCTGCAGAATCC+GGG 0.517332 4.3:-3354644 None:intergenic
TTCTAGCTCTGTGAATGAAA+TGG 0.520762 4.3:+3354511 MS.gene015851:CDS
TCCTGGATCGGTTTCTCCGG+TGG 0.521510 4.3:+3354430 MS.gene015851:CDS
ACGGCGGCGAGCTTTCTCGC+CGG 0.522012 4.3:-3354090 None:intergenic
GATTCCACCCTCTCCATCAC+AGG 0.523810 4.3:-3354740 None:intergenic
CGGTCCTTGGGAAGATCTGT+GGG 0.525368 4.3:+3354488 MS.gene015851:CDS
TTGAAGATCCGGATGTTGGG+TGG 0.525953 4.3:+3354849 MS.gene015851:CDS
CACGTGGCAGGTCACATGAC+TGG 0.526588 4.3:+3354045 MS.gene015851:CDS
CTTCCCAAGGACCGAGTCAT+CGG 0.527368 4.3:-3354479 None:intergenic
AAAATCAGGACACGAAGTGC+CGG 0.531042 4.3:-3354135 None:intergenic
GCATGTTCGAGTTTAAGATT+CGG 0.531245 4.3:+3354015 MS.gene015851:CDS
ACATTCAATCACCCAAGAAA+GGG 0.534836 4.3:-3354889 None:intergenic
CTCCGGGGACTCTGTTGAGT+CGG 0.537123 4.3:-3354320 None:intergenic
AAATGGGTTCTCCTAGATTC+GGG 0.537187 4.3:+3354597 MS.gene015851:CDS
AGTTATACTTTCTCGGGTCA+CGG 0.538971 4.3:-3354109 None:intergenic
GGGTTCGGGTTCGGGTGAAG+TGG 0.549759 4.3:+3354826 MS.gene015851:CDS
CAACTGGTTCCGTCTTTACA+CGG 0.558745 4.3:-3354248 None:intergenic
CGTACTCAGCCGTGTAAAGA+CGG 0.559308 4.3:+3354239 MS.gene015851:CDS
TGAAGATCCGGATGTTGGGT+GGG 0.561336 4.3:+3354850 MS.gene015851:CDS
CTCCGATTACTCCGATGACT+CGG 0.561730 4.3:+3354468 MS.gene015851:CDS
TTGTTCATTGTTGGCCATGG+TGG 0.564251 4.3:-3353816 None:intergenic
GTGGTTGAAGATCCGGATGT+TGG 0.565014 4.3:+3354845 MS.gene015851:CDS
AGGTGGTTCCCCTAACATCA+TGG 0.567452 4.3:-3353766 None:intergenic
GACTCAATAGCAAGCCTGAG+AGG 0.570639 4.3:-3354374 None:intergenic
GTTGGATTTGTACGATGAGG+TGG 0.581590 4.3:-3353783 None:intergenic
TGGCCATGGTGGAACGTGTA+CGG 0.582344 4.3:-3353805 None:intergenic
AATCAACGCAGTGCACACTC+CGG 0.582408 4.3:-3354337 None:intergenic
TCTCTGAGAAACTTACAACT+TGG 0.583296 4.3:+3354539 MS.gene015851:CDS
AGGAAGAGCCTGTGATGGAG+AGG 0.585551 4.3:+3354732 MS.gene015851:CDS
GGCACCTGCGTAGGTGTCGT+TGG 0.587787 4.3:-3354662 None:intergenic
CACTGCGTTGATTCGTATGA+CGG 0.588289 4.3:+3354347 MS.gene015851:CDS
CTGAGGATAATCGTCGTTGG+AGG 0.595113 4.3:-3353868 None:intergenic
CGACGACACCGATTCCGACT+CGG 0.598832 4.3:+3353941 MS.gene015851:CDS
AGAGGACCCGTGATCCGACC+CGG 0.601883 4.3:+3354626 MS.gene015851:CDS
TTGCGAGGAAGAGCCTGTGA+TGG 0.605419 4.3:+3354727 MS.gene015851:CDS
TGATTAACCCTACCACGACT+CGG 0.606063 4.3:-3354683 None:intergenic
GCAGGTGCCGAGTCGTGGTA+GGG 0.610663 4.3:+3354676 MS.gene015851:CDS
GAACTGAGGATAATCGTCGT+TGG 0.611416 4.3:-3353871 None:intergenic
CACCGCCGCCATGATGTTAG+GGG 0.612627 4.3:+3353758 None:intergenic
AGAGCCTGTGATGGAGAGGG+TGG 0.613302 4.3:+3354736 MS.gene015851:CDS
AAGACGGAACCAGTTGTCGC+CGG 0.615571 4.3:+3354255 MS.gene015851:CDS
CCACGACTCGGCACCTGCGT+AGG 0.618584 4.3:-3354671 None:intergenic
AAGATTCGGCGATGCGCACG+TGG 0.619291 4.3:+3354029 MS.gene015851:CDS
GAATCCACCGATTCATGACT+CGG 0.619620 4.3:-3353972 None:intergenic
TAGCTCTGTGAATGAAATGG+TGG 0.623336 4.3:+3354514 MS.gene015851:CDS
CCGAGAAAGTATAACTACTC+CGG 0.623467 4.3:+3354116 MS.gene015851:CDS
GGAAGAGCCTGTGATGGAGA+GGG 0.627453 4.3:+3354733 MS.gene015851:CDS
GATTCGTGTGAGTTTGCGCA+TGG 0.630448 4.3:+3354182 MS.gene015851:CDS
CCTACGCAGGTGCCGAGTCG+TGG 0.632046 4.3:+3354671 MS.gene015851:CDS
ATCGGAGTAATCGGAGACAT+CGG 0.633731 4.3:-3354461 None:intergenic
GGCTGAGTACGGTAACGAGA+AGG 0.636999 4.3:-3354227 None:intergenic
GCCAACAATGAACAATCCAA+CGG 0.640124 4.3:+3353824 MS.gene015851:CDS
GGAGTAATCGGAGACATCGG+AGG 0.641390 4.3:-3354458 None:intergenic
TGGCAGGTCACATGACTGGA+CGG 0.644811 4.3:+3354049 MS.gene015851:CDS
CATGGTGGAACGTGTACGGT+TGG 0.646215 4.3:-3353801 None:intergenic
CCTACCAACGACACCTACGC+AGG 0.651912 4.3:+3354658 MS.gene015851:CDS
GTAGGCTGCAGAATCCGGGT+CGG 0.662778 4.3:-3354640 None:intergenic
CAACCGTACACGTTCCACCA+TGG 0.668476 4.3:+3353802 MS.gene015851:CDS
TCAACGCAGTGCACACTCCG+GGG 0.673364 4.3:-3354335 None:intergenic
GACATCGGAGGAGACTCCAC+CGG 0.675579 4.3:-3354446 None:intergenic
CCTAGATTCGGGTCTCCCAG+AGG 0.678542 4.3:+3354608 MS.gene015851:CDS
GATCCAGGAGGAGACATGAA+CGG 0.679198 4.3:-3354416 None:intergenic
GAATCTTAAACTCGAACATG+CGG 0.686731 4.3:-3354013 None:intergenic
GCTTCTTGCATGTAGAACTG+AGG 0.687997 4.3:-3353885 None:intergenic
AACAATGAACAATCCAACGG+CGG 0.692559 4.3:+3353827 MS.gene015851:CDS
ACCGGAGAAACCGATCCAGG+AGG 0.697000 4.3:-3354428 None:intergenic
AGAACCCACAGATCTTCCCA+AGG 0.706781 4.3:-3354492 None:intergenic
ACGGTTGGATTTGTACGATG+AGG 0.707900 4.3:-3353786 None:intergenic
TCTTTGGGATCAGTGTTGCG+AGG 0.716443 4.3:+3354712 MS.gene015851:CDS
TATACTTTCTCGGGTCACGG+CGG 0.737764 4.3:-3354106 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GGTTAATCATTTTGATCTTT+GGG + chr4.3:3354697-3354716 MS.gene015851:CDS 25.0%
ATTTGAACATTCCATGAAGA+AGG - chr4.3:3354581-3354600 None:intergenic 30.0%
TCTTCATGGAATGTTCAAAT+GGG + chr4.3:3354581-3354600 MS.gene015851:CDS 30.0%
TTCTTCATGGAATGTTCAAA+TGG + chr4.3:3354580-3354599 MS.gene015851:CDS 30.0%
! AGCAAAGAGAACTCTTTTAA+TGG + chr4.3:3354800-3354819 MS.gene015851:CDS 30.0%
!! AGAGAACTCTTTTAATGGAT+CGG + chr4.3:3354805-3354824 MS.gene015851:CDS 30.0%
!! GGGTTAATCATTTTGATCTT+TGG + chr4.3:3354696-3354715 MS.gene015851:CDS 30.0%
CAACTTCCTTTACGAAAATC+AGG - chr4.3:3354152-3354171 None:intergenic 35.0%
GAATCTTAAACTCGAACATG+CGG - chr4.3:3354016-3354035 None:intergenic 35.0%
GGAATCTGGAAGAGATATAA+GGG + chr4.3:3354757-3354776 MS.gene015851:CDS 35.0%
TGAAGAAGGTAATGACTTCA+TGG - chr4.3:3354567-3354586 None:intergenic 35.0%
TGAAGTCATTACCTTCTTCA+TGG + chr4.3:3354567-3354586 MS.gene015851:CDS 35.0%
TGGAATCTGGAAGAGATATA+AGG + chr4.3:3354756-3354775 MS.gene015851:CDS 35.0%
! CGTTTTCGTTAACATAGTGT+TGG - chr4.3:3353923-3353942 None:intergenic 35.0%
! GATTTTTCCGAGTCATGAAT+CGG + chr4.3:3353965-3353984 MS.gene015851:CDS 35.0%
! GCATGTTCGAGTTTAAGATT+CGG + chr4.3:3354015-3354034 MS.gene015851:CDS 35.0%
! TCTCTGAGAAACTTACAACT+TGG + chr4.3:3354539-3354558 MS.gene015851:CDS 35.0%
!! GAGAACTCTTTTAATGGATC+GGG + chr4.3:3354806-3354825 MS.gene015851:CDS 35.0%
!! TTCTAGCTCTGTGAATGAAA+TGG + chr4.3:3354511-3354530 MS.gene015851:CDS 35.0%
!!! AGTTTGTTTTTTCGCTCACA+CGG + chr4.3:3354280-3354299 MS.gene015851:CDS 35.0%
AAAAATCTCCGAGTCGGAAT+CGG - chr4.3:3353952-3353971 None:intergenic 40.0%
AACAATGAACAATCCAACGG+CGG + chr4.3:3353827-3353846 MS.gene015851:CDS 40.0%
AGTTATACTTTCTCGGGTCA+CGG - chr4.3:3354112-3354131 None:intergenic 40.0%
CCGGAGTAGTTATACTTTCT+CGG - chr4.3:3354119-3354138 None:intergenic 40.0%
CGGAGTAGTTATACTTTCTC+GGG - chr4.3:3354118-3354137 None:intergenic 40.0%
CGTAAAGGAAGTTGCAAGAA+AGG + chr4.3:3354158-3354177 MS.gene015851:CDS 40.0%
GCCAACAATGAACAATCCAA+CGG + chr4.3:3353824-3353843 MS.gene015851:CDS 40.0%
GTGTGAGCGAAAAAACAAAC+TGG - chr4.3:3354281-3354300 None:intergenic 40.0%
TAATCGTCGTTGGAGGTTAA+AGG - chr4.3:3353864-3353883 None:intergenic 40.0%
! AAATGGGTTCTCCTAGATTC+GGG + chr4.3:3354597-3354616 MS.gene015851:CDS 40.0%
! CAAATGGGTTCTCCTAGATT+CGG + chr4.3:3354596-3354615 MS.gene015851:CDS 40.0%
! CCGAGAAAGTATAACTACTC+CGG + chr4.3:3354116-3354135 MS.gene015851:CDS 40.0%
! TAGCTCTGTGAATGAAATGG+TGG + chr4.3:3354514-3354533 MS.gene015851:CDS 40.0%
! TCGTGTCCTGATTTTCGTAA+AGG + chr4.3:3354143-3354162 MS.gene015851:CDS 40.0%
!! CTCTTTTAATGGATCGGGTT+CGG + chr4.3:3354811-3354830 MS.gene015851:CDS 40.0%
!! TCTTTTAATGGATCGGGTTC+GGG + chr4.3:3354812-3354831 MS.gene015851:CDS 40.0%
AAAATCAGGACACGAAGTGC+CGG - chr4.3:3354138-3354157 None:intergenic 45.0%
ACGGTTGGATTTGTACGATG+AGG - chr4.3:3353789-3353808 None:intergenic 45.0%
ACTCGGAAAAATCTCCGAGT+CGG - chr4.3:3353958-3353977 None:intergenic 45.0%
ATCGGAGTAATCGGAGACAT+CGG - chr4.3:3354464-3354483 None:intergenic 45.0%
GAACTGAGGATAATCGTCGT+TGG - chr4.3:3353874-3353893 None:intergenic 45.0%
GAGCGAAAAAACAAACTGGC+CGG - chr4.3:3354277-3354296 None:intergenic 45.0%
GCCGTTGGATTGTTCATTGT+TGG - chr4.3:3353828-3353847 None:intergenic 45.0%
GCTTCTTGCATGTAGAACTG+AGG - chr4.3:3353888-3353907 None:intergenic 45.0%
GGATTGTTCATTGTTGGCCA+TGG - chr4.3:3353822-3353841 None:intergenic 45.0%
GTTGGATTTGTACGATGAGG+TGG - chr4.3:3353786-3353805 None:intergenic 45.0%
TGATTAACCCTACCACGACT+CGG - chr4.3:3354686-3354705 None:intergenic 45.0%
TGGTTGAAGATCCGGATGTT+GGG + chr4.3:3354846-3354865 MS.gene015851:CDS 45.0%
TTGTTCATTGTTGGCCATGG+TGG - chr4.3:3353819-3353838 None:intergenic 45.0%
! CAACTGGTTCCGTCTTTACA+CGG - chr4.3:3354251-3354270 None:intergenic 45.0%
! GAACGGAAGAGTTTTCACAC+AGG - chr4.3:3354402-3354421 None:intergenic 45.0%
! GAGTGTCCTTTTTCTCATCC+CGG + chr4.3:3354071-3354090 MS.gene015851:CDS 45.0%
! TTTTCCGAGTCATGAATCGG+TGG + chr4.3:3353968-3353987 MS.gene015851:CDS 45.0%
!! CACTGCGTTGATTCGTATGA+CGG + chr4.3:3354347-3354366 MS.gene015851:CDS 45.0%
!! GAATCCACCGATTCATGACT+CGG - chr4.3:3353975-3353994 None:intergenic 45.0%
!!! TTGTTTTTTCGCTCACACGG+CGG + chr4.3:3354283-3354302 MS.gene015851:CDS 45.0%
AATCAACGCAGTGCACACTC+CGG - chr4.3:3354340-3354359 None:intergenic 50.0%
AGAACCCACAGATCTTCCCA+AGG - chr4.3:3354495-3354514 None:intergenic 50.0%
CGTACTCAGCCGTGTAAAGA+CGG + chr4.3:3354239-3354258 MS.gene015851:CDS 50.0%
CGTCTTTACACGGCTGAGTA+CGG - chr4.3:3354241-3354260 None:intergenic 50.0%
CTCCGATTACTCCGATGACT+CGG + chr4.3:3354468-3354487 MS.gene015851:CDS 50.0%
CTGAGGATAATCGTCGTTGG+AGG - chr4.3:3353871-3353890 None:intergenic 50.0%
GACCGAGTCATCGGAGTAAT+CGG - chr4.3:3354473-3354492 None:intergenic 50.0%
GACTCAATAGCAAGCCTGAG+AGG - chr4.3:3354377-3354396 None:intergenic 50.0%
GATCCAGGAGGAGACATGAA+CGG - chr4.3:3354419-3354438 None:intergenic 50.0%
GATTCGTGTGAGTTTGCGCA+TGG + chr4.3:3354182-3354201 MS.gene015851:CDS 50.0%
GGGTGAAGTGGTTGAAGATC+CGG + chr4.3:3354838-3354857 MS.gene015851:CDS 50.0%
GGTGAAAAAATCTCCGCCGT+TGG - chr4.3:3353843-3353862 None:intergenic 50.0%
GTGGTTGAAGATCCGGATGT+TGG + chr4.3:3354845-3354864 MS.gene015851:CDS 50.0%
GTTCATGTCTCCTCCTGGAT+CGG + chr4.3:3354418-3354437 MS.gene015851:CDS 50.0%
TATACTTTCTCGGGTCACGG+CGG - chr4.3:3354109-3354128 None:intergenic 50.0%
TGAAGATCCGGATGTTGGGT+GGG + chr4.3:3354850-3354869 MS.gene015851:CDS 50.0%
TTCTCGCCGGGATGAGAAAA+AGG - chr4.3:3354080-3354099 None:intergenic 50.0%
TTGAAGATCCGGATGTTGGG+TGG + chr4.3:3354849-3354868 MS.gene015851:CDS 50.0%
! TAATGGATCGGGTTCGGGTT+CGG + chr4.3:3354817-3354836 MS.gene015851:CDS 50.0%
!! TCTTTGGGATCAGTGTTGCG+AGG + chr4.3:3354712-3354731 MS.gene015851:CDS 50.0%
AAGACGGAACCAGTTGTCGC+CGG + chr4.3:3354255-3354274 MS.gene015851:CDS 55.0%
ATCAACGCAGTGCACACTCC+GGG - chr4.3:3354339-3354358 None:intergenic 55.0%
CAACCGTACACGTTCCACCA+TGG + chr4.3:3353802-3353821 MS.gene015851:CDS 55.0%
CGGTCCTTGGGAAGATCTGT+GGG + chr4.3:3354488-3354507 MS.gene015851:CDS 55.0%
CGTATGACGGTTCTCCTCTC+AGG + chr4.3:3354360-3354379 MS.gene015851:CDS 55.0%
CTTCCCAAGGACCGAGTCAT+CGG - chr4.3:3354482-3354501 None:intergenic 55.0%
CTTCCGTTCATGTCTCCTCC+TGG + chr4.3:3354413-3354432 MS.gene015851:CDS 55.0%
GAGCTGGTGAGCCCTTTCTT+GGG + chr4.3:3354878-3354897 MS.gene015851:CDS 55.0%
GATTCCACCCTCTCCATCAC+AGG - chr4.3:3354743-3354762 None:intergenic 55.0%
GGAGTAATCGGAGACATCGG+AGG - chr4.3:3354461-3354480 None:intergenic 55.0%
GGCTGAGTACGGTAACGAGA+AGG - chr4.3:3354230-3354249 None:intergenic 55.0%
TCACACGGCGGAGCAACTTA+GGG + chr4.3:3354295-3354314 MS.gene015851:CDS 55.0%
TCCGAAACCCACCCAACATC+CGG - chr4.3:3354860-3354879 None:intergenic 55.0%
TCGGTCCTTGGGAAGATCTG+TGG + chr4.3:3354487-3354506 MS.gene015851:CDS 55.0%
TGGCAGGTCACATGACTGGA+CGG + chr4.3:3354049-3354068 MS.gene015851:CDS 55.0%
TTGCGAGGAAGAGCCTGTGA+TGG + chr4.3:3354727-3354746 MS.gene015851:CDS 55.0%
! AATGGATCGGGTTCGGGTTC+GGG + chr4.3:3354818-3354837 MS.gene015851:CDS 55.0%
! AGGAAGAGCCTGTGATGGAG+AGG + chr4.3:3354732-3354751 MS.gene015851:CDS 55.0%
! CATGGTGGAACGTGTACGGT+TGG - chr4.3:3353804-3353823 None:intergenic 55.0%
! CGTTGGTAGGCTGCAGAATC+CGG - chr4.3:3354648-3354667 None:intergenic 55.0%
! GGAAGAGCCTGTGATGGAGA+GGG + chr4.3:3354733-3354752 MS.gene015851:CDS 55.0%
! GTGATGGAGAGGGTGGAATC+TGG + chr4.3:3354743-3354762 MS.gene015851:CDS 55.0%
! GTTGGTAGGCTGCAGAATCC+GGG - chr4.3:3354647-3354666 None:intergenic 55.0%
! TCCGGATGTTGGGTGGGTTT+CGG + chr4.3:3354856-3354875 MS.gene015851:CDS 55.0%
! TCCTCCTGGATCGGTTTCTC+CGG + chr4.3:3354427-3354446 MS.gene015851:CDS 55.0%
! TGGCCATGGTGGAACGTGTA+CGG - chr4.3:3353808-3353827 None:intergenic 55.0%
!! ACTCCGATGACTCGGTCCTT+GGG + chr4.3:3354476-3354495 MS.gene015851:CDS 55.0%
!! TACTCCGATGACTCGGTCCT+TGG + chr4.3:3354475-3354494 MS.gene015851:CDS 55.0%
AAGATTCGGCGATGCGCACG+TGG + chr4.3:3354029-3354048 MS.gene015851:CDS 60.0%
ACAAACTGGCCGGCGACAAC+TGG - chr4.3:3354267-3354286 None:intergenic 60.0%
ACCGGAGAAACCGATCCAGG+AGG - chr4.3:3354431-3354450 None:intergenic 60.0%
CACGTGGCAGGTCACATGAC+TGG + chr4.3:3354045-3354064 MS.gene015851:CDS 60.0%
CAGAATCCGGGTCGGATCAC+GGG - chr4.3:3354635-3354654 None:intergenic 60.0%
CCTACCAACGACACCTACGC+AGG + chr4.3:3354658-3354677 MS.gene015851:CDS 60.0%
CCTAGATTCGGGTCTCCCAG+AGG + chr4.3:3354608-3354627 MS.gene015851:CDS 60.0%
CGCATGGTGTGTTCGAGTGC+TGG + chr4.3:3354198-3354217 MS.gene015851:CDS 60.0%
CTCACACGGCGGAGCAACTT+AGG + chr4.3:3354294-3354313 MS.gene015851:CDS 60.0%
CTCCGACTCAACAGAGTCCC+CGG + chr4.3:3354318-3354337 MS.gene015851:CDS 60.0%
CTCCGGGGACTCTGTTGAGT+CGG - chr4.3:3354323-3354342 None:intergenic 60.0%
GACATCGGAGGAGACTCCAC+CGG - chr4.3:3354449-3354468 None:intergenic 60.0%
GCAGAATCCGGGTCGGATCA+CGG - chr4.3:3354636-3354655 None:intergenic 60.0%
GGAGCTGGTGAGCCCTTTCT+TGG + chr4.3:3354877-3354896 MS.gene015851:CDS 60.0%
GTAGGCTGCAGAATCCGGGT+CGG - chr4.3:3354643-3354662 None:intergenic 60.0%
TCAACGCAGTGCACACTCCG+GGG - chr4.3:3354338-3354357 None:intergenic 60.0%
TCCACCGGAGAAACCGATCC+AGG - chr4.3:3354434-3354453 None:intergenic 60.0%
! AGAGCCTGTGATGGAGAGGG+TGG + chr4.3:3354736-3354755 MS.gene015851:CDS 60.0%
! CCTCTGGGAGACCCGAATCT+AGG - chr4.3:3354611-3354630 None:intergenic 60.0%
! TCCTGGATCGGTTTCTCCGG+TGG + chr4.3:3354430-3354449 MS.gene015851:CDS 60.0%
! TGTTGGGTGGGTTTCGGAGC+TGG + chr4.3:3354862-3354881 MS.gene015851:CDS 60.0%
!! CCTGCGTAGGTGTCGTTGGT+AGG - chr4.3:3354661-3354680 None:intergenic 60.0%
!! CGACGACACCGATTCCGACT+CGG + chr4.3:3353941-3353960 MS.gene015851:CDS 60.0%
AGAGGACCCGTGATCCGACC+CGG + chr4.3:3354626-3354645 MS.gene015851:CDS 65.0%
GGTCGGATCACGGGTCCTCT+GGG - chr4.3:3354626-3354645 None:intergenic 65.0%
! GGCACCTGCGTAGGTGTCGT+TGG - chr4.3:3354665-3354684 None:intergenic 65.0%
! GGGTTCGGGTTCGGGTGAAG+TGG + chr4.3:3354826-3354845 MS.gene015851:CDS 65.0%
!! GCAGGTGCCGAGTCGTGGTA+GGG + chr4.3:3354676-3354695 MS.gene015851:CDS 65.0%
ACGGCGGCGAGCTTTCTCGC+CGG - chr4.3:3354093-3354112 None:intergenic 70.0%
GGGTCGGATCACGGGTCCTC+TGG - chr4.3:3354627-3354646 None:intergenic 70.0%
TTCGGCGATGCGCACGTGGC+AGG + chr4.3:3354033-3354052 MS.gene015851:CDS 70.0%
! CCACGACTCGGCACCTGCGT+AGG - chr4.3:3354674-3354693 None:intergenic 70.0%
!! CCTACGCAGGTGCCGAGTCG+TGG + chr4.3:3354671-3354690 MS.gene015851:CDS 70.0%
!! CGCAGGTGCCGAGTCGTGGT+AGG + chr4.3:3354675-3354694 MS.gene015851:CDS 70.0%
CGGCGGCGAGCTTTCTCGCC+GGG - chr4.3:3354092-3354111 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 3353768 3354907 3353768 ID=MS.gene015851
chr4.3 mRNA 3353768 3354907 3353768 ID=MS.gene015851.t1;Parent=MS.gene015851
chr4.3 exon 3353768 3354907 3353768 ID=MS.gene015851.t1.exon1;Parent=MS.gene015851.t1
chr4.3 CDS 3353768 3354907 3353768 ID=cds.MS.gene015851.t1;Parent=MS.gene015851.t1
Gene Sequence

>MS.gene015851

ATGATGTTAGGGGAACCACCTCATCGTACAAATCCAACCGTACACGTTCCACCATGGCCAACAATGAACAATCCAACGGCGGAGATTTTTTCACCTTTAACCTCCAACGACGATTATCCTCAGTTCTACATGCAAGAAGCACTAAGCGCTTTCCAACACTATGTTAACGAAAACGACGACACCGATTCCGACTCGGAGATTTTTCCGAGTCATGAATCGGTGGATTCGTATTCTAACGATCATTTCCGCATGTTCGAGTTTAAGATTCGGCGATGCGCACGTGGCAGGTCACATGACTGGACGGAGTGTCCTTTTTCTCATCCCGGCGAGAAAGCTCGCCGCCGTGACCCGAGAAAGTATAACTACTCCGGCACTTCGTGTCCTGATTTTCGTAAAGGAAGTTGCAAGAAAGGTGATTCGTGTGAGTTTGCGCATGGTGTGTTCGAGTGCTGGCTTCATCCTTCTCGTTACCGTACTCAGCCGTGTAAAGACGGAACCAGTTGTCGCCGGCCAGTTTGTTTTTTCGCTCACACGGCGGAGCAACTTAGGGCTCCGACTCAACAGAGTCCCCGGAGTGTGCACTGCGTTGATTCGTATGACGGTTCTCCTCTCAGGCTTGCTATTGAGTCTTCCTGTGTGAAAACTCTTCCGTTCATGTCTCCTCCTGGATCGGTTTCTCCGGTGGAGTCTCCTCCGATGTCTCCGATTACTCCGATGACTCGGTCCTTGGGAAGATCTGTGGGTTCTAGCTCTGTGAATGAAATGGTGGCTTCTCTGAGAAACTTACAACTTGGTACCATGAAGTCATTACCTTCTTCATGGAATGTTCAAATGGGTTCTCCTAGATTCGGGTCTCCCAGAGGACCCGTGATCCGACCCGGATTCTGCAGCCTACCAACGACACCTACGCAGGTGCCGAGTCGTGGTAGGGTTAATCATTTTGATCTTTGGGATCAGTGTTGCGAGGAAGAGCCTGTGATGGAGAGGGTGGAATCTGGAAGAGATATAAGGGTGAAGATGTTTGAGAAACTGAGCAAAGAGAACTCTTTTAATGGATCGGGTTCGGGTTCGGGTGAAGTGGTTGAAGATCCGGATGTTGGGTGGGTTTCGGAGCTGGTGAGCCCTTTCTTGGGTGATTGA
Protein sequence

>MS.gene015851.t1

MMLGEPPHRTNPTVHVPPWPTMNNPTAEIFSPLTSNDDYPQFYMQEALSAFQHYVNENDDTDSDSEIFPSHESVDSYSNDHFRMFEFKIRRCARGRSHDWTECPFSHPGEKARRRDPRKYNYSGTSCPDFRKGSCKKGDSCEFAHGVFECWLHPSRYRTQPCKDGTSCRRPVCFFAHTAEQLRAPTQQSPRSVHCVDSYDGSPLRLAIESSCVKTLPFMSPPGSVSPVESPPMSPITPMTRSLGRSVGSSSVNEMVASLRNLQLGTMKSLPSSWNVQMGSPRFGSPRGPVIRPGFCSLPTTPTQVPSRGRVNHFDLWDQCCEEEPVMERVESGRDIRVKMFEKLSKENSFNGSGSGSGEVVEDPDVGWVSELVSPFLGD