Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015930.t1 | RHN76864.1 | 76.3 | 755 | 159 | 9 | 1 | 735 | 1 | 755 | 0.00E+00 | 1080.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015930.t1 | Q9LMN8 | 44.5 | 733 | 358 | 19 | 30 | 735 | 31 | 741 | 2.4e-144 | 514.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015930.t1 | A0A396JI89 | 76.3 | 755 | 159 | 9 | 1 | 735 | 1 | 755 | 0.0e+00 | 1080.5 |
TFs/TRs:
Protein Kinases:
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| No data available in table | ||||
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015930.t1 | MTR_1g010260 | 74.172 | 755 | 151 | 10 | 1 | 735 | 1 | 731 | 0.0 | 1095 |
| MS.gene015930.t1 | MTR_1g010220 | 68.384 | 718 | 193 | 12 | 6 | 698 | 1 | 709 | 0.0 | 951 |
| MS.gene015930.t1 | MTR_1g027580 | 61.580 | 747 | 266 | 12 | 2 | 735 | 6 | 744 | 0.0 | 898 |
| MS.gene015930.t1 | MTR_1g027570 | 61.518 | 738 | 265 | 10 | 11 | 735 | 13 | 744 | 0.0 | 895 |
| MS.gene015930.t1 | MTR_1g027660 | 60.656 | 732 | 267 | 11 | 14 | 735 | 1 | 721 | 0.0 | 867 |
| MS.gene015930.t1 | MTR_1g010240 | 62.500 | 752 | 155 | 13 | 4 | 735 | 1 | 645 | 0.0 | 861 |
| MS.gene015930.t1 | MTR_1g027600 | 59.264 | 734 | 283 | 10 | 12 | 735 | 20 | 747 | 0.0 | 848 |
| MS.gene015930.t1 | MTR_1g027490 | 60.458 | 698 | 259 | 8 | 30 | 716 | 18 | 709 | 0.0 | 844 |
| MS.gene015930.t1 | MTR_1g027640 | 58.971 | 719 | 275 | 11 | 30 | 735 | 38 | 749 | 0.0 | 826 |
| MS.gene015930.t1 | MTR_1g010250 | 92.163 | 319 | 25 | 0 | 417 | 735 | 309 | 627 | 0.0 | 600 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene015930.t1 | AT1G21270 | 43.369 | 754 | 383 | 20 | 4 | 735 | 1 | 732 | 0.0 | 555 |
| MS.gene015930.t1 | AT1G21240 | 44.399 | 741 | 347 | 20 | 30 | 735 | 31 | 741 | 0.0 | 554 |
| MS.gene015930.t1 | AT1G21250 | 45.157 | 733 | 346 | 24 | 30 | 735 | 32 | 735 | 0.0 | 553 |
| MS.gene015930.t1 | AT1G21230 | 44.636 | 755 | 373 | 22 | 4 | 735 | 1 | 733 | 0.0 | 550 |
| MS.gene015930.t1 | AT1G21210 | 40.261 | 765 | 397 | 21 | 4 | 735 | 1 | 738 | 6.07e-174 | 516 |
| MS.gene015930.t1 | AT1G16130 | 39.943 | 701 | 361 | 22 | 11 | 678 | 17 | 690 | 6.28e-146 | 445 |
| MS.gene015930.t1 | AT1G79670 | 38.260 | 724 | 369 | 24 | 4 | 678 | 1 | 695 | 5.37e-139 | 427 |
| MS.gene015930.t1 | AT1G16120 | 39.199 | 699 | 349 | 20 | 26 | 678 | 35 | 703 | 3.03e-138 | 424 |
| MS.gene015930.t1 | AT1G16150 | 36.993 | 765 | 391 | 24 | 11 | 713 | 14 | 749 | 8.20e-137 | 421 |
| MS.gene015930.t1 | AT1G79680 | 38.069 | 725 | 338 | 20 | 26 | 678 | 23 | 708 | 3.68e-134 | 415 |
Find 148 sgRNAs with CRISPR-Local
Find 317 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGTGATGTCTATAGCTT+TGG | 0.378017 | 1.2:-9729991 | MS.gene015930:CDS |
| AAACAAACAAGAGATCAAGG+AGG | 0.782860 | 1.2:-9729821 | MS.gene015930:CDS |
| AAAGAACTTCTCTTTCAGTT+TGG | 0.361439 | 1.2:+9730665 | None:intergenic |
| AAATTATTCAAGATGGTTTG+TGG | 0.418828 | 1.2:-9729852 | MS.gene015930:CDS |
| AAATTGACCTGTGCAGCCAT+TGG | 0.488754 | 1.2:+9735523 | None:intergenic |
| AACCTGGATTGCAATGGCAT+CGG | 0.587199 | 1.2:+9735567 | None:intergenic |
| AACTACCTGTGGTTCTTGAC+TGG | 0.502019 | 1.2:-9735688 | MS.gene015930:CDS |
| AACTGAAAGAGAAGTTCTTT+AGG | 0.431457 | 1.2:-9730662 | MS.gene015930:CDS |
| AAGACCAAGCATGAAGGAAG+TGG | 0.554397 | 1.2:-9729755 | MS.gene015930:CDS |
| AAGATAGCTGCAGAAGTAGC+TGG | 0.436907 | 1.2:-9730231 | MS.gene015930:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAAATGCCGAATTTCAAAG+AGG | - | chr1.2:9733866-9733885 | MS.gene015930:intron | 30.0% | |
| AAAAGTGATGTCTATAGCTT+TGG | - | chr1.2:9735904-9735923 | MS.gene015930:CDS | 30.0% | |
| AAAGAACTTCTCTTTCAGTT+TGG | + | chr1.2:9735233-9735252 | None:intergenic | 30.0% | |
| AACTGAAAGAGAAGTTCTTT+AGG | - | chr1.2:9735233-9735252 | MS.gene015930:intron | 30.0% | |
| ACAAACTCATAAACCAACAA+AGG | + | chr1.2:9735571-9735590 | None:intergenic | 30.0% | |
| ACACATTATGTGTGAAAAAG+TGG | + | chr1.2:9730868-9730887 | None:intergenic | 30.0% | |
| ACATTATGTGTGAAAAAGTG+GGG | + | chr1.2:9730866-9730885 | None:intergenic | 30.0% | |
| AGAAAACAAACAAGAGATCA+AGG | - | chr1.2:9736071-9736090 | MS.gene015930:CDS | 30.0% | |
| AGAACTTGAACTGATAGTAT+TGG | - | chr1.2:9736323-9736342 | MS.gene015930:CDS | 30.0% | |
| AGAAGTGATATTTGTCAAAC+AGG | + | chr1.2:9733721-9733740 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 9729558 | 9736359 | 9729558 | ID=MS.gene015930 |
| chr1.2 | mRNA | 9729558 | 9736359 | 9729558 | ID=MS.gene015930.t1;Parent=MS.gene015930 |
| chr1.2 | exon | 9735531 | 9736359 | 9735531 | ID=MS.gene015930.t1.exon1;Parent=MS.gene015930.t1 |
| chr1.2 | CDS | 9735531 | 9736359 | 9735531 | ID=cds.MS.gene015930.t1;Parent=MS.gene015930.t1 |
| chr1.2 | exon | 9730916 | 9731092 | 9730916 | ID=MS.gene015930.t1.exon2;Parent=MS.gene015930.t1 |
| chr1.2 | CDS | 9730916 | 9731092 | 9730916 | ID=cds.MS.gene015930.t1;Parent=MS.gene015930.t1 |
| chr1.2 | exon | 9729558 | 9730759 | 9729558 | ID=MS.gene015930.t1.exon3;Parent=MS.gene015930.t1 |
| chr1.2 | CDS | 9729558 | 9730759 | 9729558 | ID=cds.MS.gene015930.t1;Parent=MS.gene015930.t1 |
| Gene Sequence |
>MS.gene015930 ATGAAAGTGATGAAGATGAAGCATATGTATGTGTTAGCCATGGTGATACTGGTAGGAACTGTTGCAGCTGATTTAGCAACAAATACCTGCAGTCACACTTGTGGAGGTCAAGAAATTCAATATCCATTTGCCATAAACGACACTTCATCAGATTCAGATTGTTTCTTGGGTGGTAAACTAATTCCGCTCACTTGCAATGGATCCAAAATATACTTGGGTAACATGGAAGTGTTAAACATAACCATCTCCAAAGCTGAAATTGACGTGTTATTTTACGTCTCTGAGTACTGCGGTGTTGATGATAATACTCGAGCTACGCTTGAAAGCGATTCATATACCATTTCAAGCAAAGAAAACAAGTTTGTAACTGTTGGTTCTAACAGTTACGGTTATTTTTATGTTTTCGATGGTGACACGTATTCAACTGGGTGCTTAACACGCTTTTTTGATCATCAAAGATCGATTGATAATGGAACATGTTCAGGTGTAGGGTGTTGCCAAGTTGATATTCCTCCTAGAATGTGGAATATAAGTATAGAAGCTTTTAATTTCAACAATGAATCCGATTTTTGTAGCTATGCATTTGTTGTTAAGAATGGTAGGTATAATTTTTCTTTAGCTCATTTATCACAAGGTTTTCCTTTCAAGGAACTACCTGTGGTTCTTGACTGGACAATTGGCAACGAAAGCTGCAGTACAGCTTCTAATGGAGTCAACTATGGGTGCAAGAAGAATAGCTATTGTGATGACAAGGACACATATTTTGGTTACCGATGCCATTGCAATCCAGGTTTTGAAGGAAATCCTTATCATCCCAATGGCTGCACAGGTCAATTTTTTATTCAAATTTTGGCAATTTACGTTTATTTTCATTTCTTCTTTAAACTTGGTATCGGTTTAATTACGTACTAAACTACTAATCCAAAGGTCCAGTTTCACTGCCTATGTGCGACTGCGCTATTTAAAACTCTTTGTATGGATTCCACAAAATTAACATTGAAGGATCGAACCTGAGACTTAAAAAAAAAAAAAAAAAAAAAAAACACACTCCAAAATCTTAACTAAACCACCAAACCAATCCAAATATGTTTATAGTACAATATTGTTGATCTTTAGTGCTTTAATTAAGGTTCTGAAAATCAGACCCGTCCTTGATCAAGTCGTTCCAAGATTCAATAATTCAATGATAGCTAAATTTTGAAATTTTATAGTAGTGAATAAAAACATACATTGTCCCGTATGTGTAGCCCAGTTGGTAGCTACCAGGGACAATTTGAGCAGGGGACGGGGTTTGAATCCCAGATTCCCCACTTTTTCACACATAATGTGTGTGAATCTTGTCACTAGGCTACTTGACAAAAAAAAAAACATACTTAACATTTTTTTATATAATAATGATATTAATTATTACTACAAGTCTACAAGGGTATAAATGAAATTTATCATTTAATATATTTGAAATTTAATCAACGTCAATAGTATCAATAGTGTGTGTCTATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATAAAGTAATGTTTTTTATGTCACGTGTCACTTCCACGTTTATTCTCAATATTTTTTTATTTTTTAAAATCAATATTTTATTGGTATAAATATCTCAGCAAACTTTATATCGTATCCACTCAATATTTTTTCTTTTGAATTTCTAATATTTTATGCTGCAGAAATATCTCAACAAACTAGATATAATACTTTCAACAGAGTTTATATATATGATCTTATTTTATGATAAAAAGGCTCCAATCGGTTTGTTTTTTTTTTAGGAAAAGAAGCTTCAATTAGTTACTAAGTAATCTTTTATCACCGTATTATTCAATTAGCTTTTATTTTTGAGGAAAAAAGGCTTCGATTAGTTATTATGTTATCTTTTAACAGCATATTCTAATGCAAATGTTAACTATAAATAAAGGAATTTTTGTTAACCACATATTTTGAGCACAAACGAAAATTTCAAATCTTCTTTTCATCAAACATTTTTCCTTCTTCGGGTTCGCCAAACACCAATTCTATCATTTCTCTTACATATACTTTGATTTTTAATATATATATATATATTTCAACCACTTTGTAATGCTACTTTTTAATATAACATTTTTTCATCTTTTTGTTATTTTTATTTTTTAATTTTTGAAGACAACTTTTCAAACATCGTAAACACTTCTACTAATGTTTATACACACACACACTTACAATACTTTGATTCAATTTTGATTCTCATTTAACCAAATCATAAATTTCATCATTTTGTTTTTTATATTTCTTTTTCAGAACTTATGGCTACAAAATGTGACTTCATTGATGACGTCAATCAATCAGTATCAATAGTATATAAATAAAGAAAGATATTTTATGTCACGTTTAGACTCCACGTTTATTCTCAATTTTTATATTAATCTTCTATTTTTACATGAAGATTTAATTTTGAATAGATATCTGAACAAATTTCTTCTTTTCATCAAAAAAATATCTTCTTCTTTCTATTTAGTATATATCTCCCTCTCTCAAAAAACTATATAAATTACATTGCTATTTTCCAATAAATATATTACTTCATTATCTGATTAATAATATTTTTTTCTTACAAATGTTAACTATAGATAAAGGAATTTTTGTTAACCACATATTTTGAGCACAAACGAAAATCTCAAATCTTCTTTTCATCAAACATTTTTCCTTCTTCGGGTTCGCCAAACACCAATTCTATCATTTCTCTTACATATACTTTGATTTTTAATATATATATATTTCAACCACTTTGTAATGCTACTTTTTAATATAACATTTTTTCATCTTTTTGTTATTTTTATTTTTAAATTTTTGAAGACAACTTTTCAAACATCGTAAACACTTCTACTGATGTTTATACACACACACTTACAATACTTTGATTCAATTTTGATTCTCATTTAACAAATCATAAATTTCATCATTTTGTTTTTTATATTTCTTTTTCAAAAATTATGGCTACAAAATTTGACTTCATTGATGACGTCAATCAATCAATATCAATAGTATATAAATAAAGTAAGATATTTTATGTCACGTTTCGACTCCACGTTTATTCTCAATTTTTATATTAATCTTCTATTTTTATGTGAAGATTTAATTTTGCATAGATATCTGAACAAATTTCTTCTTTTCATCAAAAAAAAATTCTTCTTCTTTCTATTTAGTATATATCTCCCTCTCTCAAAAAACTATATAAATTACATTGATATTTTCCAATAAATATATTACTTCATTATCTGATTAAATAATACTTTTTTCGTACAATCGATAAAAGTATAATTTAATTTTATCATAATTTATTTTAATAGTTTATTTTTTTTCCATATTCAATATTTTTGTTTCTTTCACATTTGTTTTGAATATTTTGATTTTTGAATAATATTTATTTTAATTTGATATAAATATACCAATGTACATTTTTTTAATGTTTTCATAATATTATTTTTTAATGTTTTCATAATATTATATTTTTTATTTAAATAAACTTATTATTTTTTGAATTTTCTAAATATTTTATTTATTATTTTTTATGAAGTAATAATTTTTTTTTTGTGGTGGCCGGGGTTTGAACCCCGGACTTTGCATATTTTATGCATTGTCCCTACCAACTGAGCTATGCTCACGAGAACATGTAGCAATAATATCTCAACAAGCTAAATATCTTATATATATACTTTTTTGTTTTTCTTATATCTTATCTTTTAAAAACTAATTTTTATAATTGCTTATATTATTTGTTACTTCTTTTTATTTATCTTTTATCATCATATTCCAATTCAAACTTTCACAATAAAACTAGGTAAAAAAATTATTTCCATTTTTTAATGCTGTTTTATTTTATTACTTTATCATAGTTTATTTCAATAAATTTTTTATTTTTTATTATTTTTTATTATTTTTCAAATTTTTAGTATATTTTATTTTTTTTATGTAACATAAATATCTCAACAAGCTAAATATATTATATCCTTTTTTTTTAAAATTGCTTAAATTATTTGTTATTTCTTTATCTTTTAGCATCATATGCCAATTCAAATTTTAACTACTCACATAATTTTAGCACAAGTAGAATTACACCTTTGAAATCAACCTTCTCATTCCTGTTTGACAAATATCACTTCTATAATTTCTTTTTCATACACTTTTTCATATACTATTGATTTTTTTTTTTTGCAGAAACTTTATTAACATTATTCACACTCTAAATATAAAATATGGAGACCCAAGATTAGGATCATTAGAATGTGAAAAATGCCGAATTTCAAAGAGGTCACATAAATTTAATCGATTTGATTTTTATGTATGATAGAGCATGCATCAAATTTTTTTTCTTCGTTAAACTTTTAATAACATGATTTCACATTAACTTAATTATTTTTCTATGCTAATATTTATTTAATTTTGTTGGTGGTGATATTCTAAGGGATCAAAATTTAATGCACTGTGTGAAGCGACAATGTTACAAAATATTAATCTGTTATTATAAAAAAAAAATATCAATCTACGTTGATTGATGTAATATGTTACGAGATAAAAAAAAGTATCTTAATTGCTAATAACAACCGTAGATTTAAACGAACTTCCTATGAATATAGAATATTTTATAAAACTATAACCATTGTGAAGAAGCATAATTATTTTCAAGGAGTTAATTTATATGTCTTTTGTCTATTTATTCGTTATACTTTTTTTTTGGGTAACCATGATACATGTACACAAATTCCACCATCGTTAGCGATGACTAATCTCCGTCAGGAATCGGTGGGGCACAAAAGGTGGGTTCTTCCCTTTTAATCGATTTTTTTACTACGCATGAGCCACGAAACAAACTCATCACCACATGTTTAAAGGGACCCAAAACTCGTACTATTTGAACTAATTTGTTATTTTTAAGATATTATTATTATTGTAAATTATTCTTAATTATTTACACCATCTCACCAAAAATACCCAAAACTCATCTCACCATTTATAATTATTTTTAAGATATTATTATTATTGTAAATTTAAATAAATTTAATTTAATATACTTACATGTGTATTCGTGCACACAAACTTATTTAATATCTAACCCGGGCAACGTCCGGGCTCTAAACTAGTTTCTTATAAAAATAATTTGATAACTATTTACAACGAAGAATATCTAAATCAAAAGACCGTAGTGAGTGAAAGAAAAAAGTTAAAATTCTATTTTATGGGCATACAGATATTGACGAATGTGAAAGAAACTCACATGGATGCGATGTCATAGCAAAATGTAAGAACACTTATGGGAATTACACATGTGAGTGTCCAAAGGGGTATTTCGGAGATGGAACAAAGGAAAGAGGGTGCCAACTACATGATGCAAATGCAAATTCAATAGTACTACCTACTGTCCTTGGTAAGCAACTAAGCATGCCCTATTGATTTTGTTTAAATTGTTTTTTTTTCACTTTTTTATAATCCTTAGTGACATGCATGTTCATTTACTATGCAATAGATATTATTTAGTTAAGTGAACTATTCATAAACTGGGTTTGATATATTTTGAATGCAGGAGTAGGAGCAGCATTTATTATTCTATTTGTGGGGATAGTTTTCTTCATAATATACCAAAAAAGGAAATTTACCAAACTGAAAGAGAAGTTCTTTAGGCAGAATGGAGGTTCTATTTTGGAACAAAAACTTCTTCAAAGAAAAGACTCGTCTCAAATAGCTCACATTTTCAAAGAAAATGAACTAAGGAAAGCCACTAACAACTTTGATGAGAGCTTAATCATAGGTAAAGGAGGTTTTGGTACAGTTTTCAAAGGGGAACTAAATGATAATAGAATTGTTGCTATCAAAAAGTCCAAAACAATAGATGAAAGCCAAATTGAACAATTCATTAATGAGGTGGATGTTGTTTCTCAAATAAATCACAGGAATGTGGTCAAACTCTTAGGGTGTTGTCTAGAAACAGAAGTTCCTTTGTTGGTTTATGAGTTTGTTAGCAATGGTACTCTTTCTGAATTTATACACACACAAGGTAAGATGAACGATCCAACTTGGAAAACTCGTCTTAAGATAGCTGCAGAAGTAGCTGGAGCTCTGTCATATTTGCATTCAGCTGCCTCAATACCCATTATCCATAGAGATGTCAAGAGTGCTAACATTCTCTTAGATGGAACCAACACTGCTAAAGTATCAGATTTTGGAGCTTCAAGATTGGTTCCAATCGATCAAACTGAAGTAGCCACAATGGTGCAAGGAACTATTGGCTACTTGGATCCAGAGTATATGCAAACAAGCCAATTAACTGAAAAAAGTGATGTCTATAGCTTTGGAGTAGTTCTTGTGGAGTTGCTAACAGGGGAGAAGCCTTTTAGTTTTGGTAGGCCGGAAGAGAGAAGAAGTCTTGCTATGCATTTTCTGTCTTGCTTGGAAAAGGATAATGTGTTTGAAATTATTCAAGATGGTTTGTGGAATGAAGAAAACAAACAAGAGATCAAGGAGGTGGCTGTTCTTGCGGCGAAGTGTTTGAGACTTAGAGGGGAGGAAAGACCAAGCATGAAGGAAGTGGCTATGGAATTGGAGGGAATGAGGTTAATGGATAAGCACTCTTGGATCAATGATGACTTAAATGTAGAGGAGAGCCGGTGCTTGCTTCATGAATCATCATCTAGCCTCTATGAACCTGGTGATAGCAGCAAACATGGAGATATCAGATATGACAGCCTGAAAGAACTTGAACTGATAGTATTGGACGATGGAAGGTGA |
| Protein sequence |
>MS.gene015930.t1 MKVMKMKHMYVLAMVILVGTVAADLATNTCSHTCGGQEIQYPFAINDTSSDSDCFLGGKLIPLTCNGSKIYLGNMEVLNITISKAEIDVLFYVSEYCGVDDNTRATLESDSYTISSKENKFVTVGSNSYGYFYVFDGDTYSTGCLTRFFDHQRSIDNGTCSGVGCCQVDIPPRMWNISIEAFNFNNESDFCSYAFVVKNGRYNFSLAHLSQGFPFKELPVVLDWTIGNESCSTASNGVNYGCKKNSYCDDKDTYFGYRCHCNPGFEGNPYHPNGCTDIDECERNSHGCDVIAKCKNTYGNYTCECPKGYFGDGTKERGCQLHDANANSIVLPTVLGVGAAFIILFVGIVFFIIYQKRKFTKLKEKFFRQNGGSILEQKLLQRKDSSQIAHIFKENELRKATNNFDESLIIGKGGFGTVFKGELNDNRIVAIKKSKTIDESQIEQFINEVDVVSQINHRNVVKLLGCCLETEVPLLVYEFVSNGTLSEFIHTQGKMNDPTWKTRLKIAAEVAGALSYLHSAASIPIIHRDVKSANILLDGTNTAKVSDFGASRLVPIDQTEVATMVQGTIGYLDPEYMQTSQLTEKSDVYSFGVVLVELLTGEKPFSFGRPEERRSLAMHFLSCLEKDNVFEIIQDGLWNEENKQEIKEVAVLAAKCLRLRGEERPSMKEVAMELEGMRLMDKHSWINDDLNVEESRCLLHESSSSLYEPGDSSKHGDIRYDSLKELELIVLDDGR |
