AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene015994

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015994.t1 MTR_1g012610 95.687 626 26 1 1 625 6 631 0.0 1241
MS.gene015994.t1 MTR_1g012610 95.687 626 26 1 1 625 15 640 0.0 1241
MS.gene015994.t1 MTR_1g012610 95.687 626 26 1 1 625 16 641 0.0 1238
MS.gene015994.t1 MTR_0137s0080 30.488 328 193 7 14 311 114 436 2.65e-36 145
MS.gene015994.t1 MTR_3g014090 31.561 301 174 6 38 311 141 436 3.66e-36 146
MS.gene015994.t1 MTR_3g014090 31.561 301 174 6 38 311 139 434 4.06e-36 145
MS.gene015994.t1 MTR_5g023150 26.351 296 194 10 21 311 178 454 5.15e-22 100
MS.gene015994.t1 MTR_5g023150 26.351 296 194 10 21 311 275 551 8.04e-22 100
MS.gene015994.t1 MTR_4g088755 29.798 198 131 3 119 315 116 306 5.40e-20 92.4
MS.gene015994.t1 MTR_4g088745 29.798 198 131 3 119 315 121 311 5.59e-20 92.4
MS.gene015994.t1 MTR_4g088750 28.788 198 133 3 119 315 277 467 2.40e-18 89.0
MS.gene015994.t1 MTR_5g066490 25.166 302 204 8 19 313 623 909 3.76e-18 89.4
MS.gene015994.t1 MTR_2g013900 29.798 198 131 3 119 315 238 428 8.51e-18 87.0
MS.gene015994.t1 MTR_1g113960 24.585 301 201 11 25 319 285 565 1.13e-16 84.0
MS.gene015994.t1 MTR_3g100090 25.407 307 204 9 19 315 630 921 1.87e-16 84.0
MS.gene015994.t1 MTR_3g101210 26.642 274 179 8 46 310 313 573 8.01e-16 81.3
MS.gene015994.t1 MTR_3g101210 26.642 274 179 8 46 310 313 573 9.49e-16 81.3
MS.gene015994.t1 MTR_1g113960 25.338 296 191 12 25 314 285 556 9.76e-16 80.9
MS.gene015994.t1 MTR_3g078110 22.468 316 206 8 15 305 34 335 1.50e-15 79.0
MS.gene015994.t1 MTR_8g107000 25.180 278 183 9 33 305 725 982 6.67e-14 75.9
MS.gene015994.t1 MTR_2g020380 35.036 137 84 4 130 263 81 215 8.05e-14 75.5
MS.gene015994.t1 MTR_4g049730 28.313 166 106 5 139 302 91 245 8.65e-14 73.9
MS.gene015994.t1 MTR_5g006560 25.543 184 130 3 119 301 181 358 1.09e-13 73.6
MS.gene015994.t1 MTR_1g103270 23.280 189 138 3 119 306 173 355 1.24e-13 73.6
MS.gene015994.t1 MTR_5g006560 25.543 184 130 3 119 301 215 392 1.90e-13 73.2
MS.gene015994.t1 MTR_4g132860 25.758 198 138 4 119 315 155 344 2.74e-13 72.4
MS.gene015994.t1 MTR_6g079540 30.435 161 104 4 147 305 511 665 2.77e-13 73.6
MS.gene015994.t1 MTR_6g079540 30.435 161 104 4 147 305 759 913 3.00e-13 73.6
MS.gene015994.t1 MTR_4g082325 24.837 306 203 11 21 314 121 411 4.03e-13 72.0
MS.gene015994.t1 MTR_7g092360 23.684 266 189 6 38 302 14 266 5.40e-13 72.4
MS.gene015994.t1 MTR_5g010730 28.409 176 110 5 147 312 522 691 1.22e-12 71.2
MS.gene015994.t1 MTR_5g010730 28.409 176 110 5 147 312 577 746 1.68e-12 70.9
MS.gene015994.t1 MTR_3g114900 22.545 275 178 5 40 304 270 519 1.84e-12 70.5
MS.gene015994.t1 MTR_1g081790 24.522 314 198 10 10 305 650 942 2.50e-12 70.5
MS.gene015994.t1 MTR_1g061190 27.972 286 177 11 31 308 549 813 2.76e-12 70.5
MS.gene015994.t1 MTR_5g059880 27.976 168 107 5 135 301 286 440 3.67e-11 66.2
MS.gene015994.t1 MTR_2g023890 27.574 272 148 13 46 301 11 249 4.87e-11 65.1
MS.gene015994.t1 MTR_2g040510 25.500 200 130 7 115 302 138 330 5.51e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene015994.t1 AT4G32250 63.542 576 196 7 10 580 14 580 0.0 739
MS.gene015994.t1 AT4G32250 63.542 576 196 7 10 580 14 580 0.0 739
MS.gene015994.t1 AT4G32250 63.542 576 196 7 10 580 14 580 0.0 739
MS.gene015994.t1 AT5G13530 32.226 301 171 9 40 311 137 433 2.06e-36 147
MS.gene015994.t1 AT5G13530 32.226 301 171 9 40 311 137 433 2.22e-36 147
MS.gene015994.t1 AT4G24480 26.866 335 204 10 8 316 624 943 3.35e-19 92.8
MS.gene015994.t1 AT4G24480 26.888 331 204 10 8 316 624 938 2.32e-18 90.1
MS.gene015994.t1 AT4G24480 26.888 331 204 10 8 316 624 938 2.54e-18 90.1
MS.gene015994.t1 AT4G35780 23.453 307 209 11 15 314 264 551 9.27e-18 87.4
MS.gene015994.t1 AT4G38470 24.516 310 210 10 8 312 257 547 1.63e-17 86.7
MS.gene015994.t1 AT4G38470 25.161 310 204 11 8 312 257 543 3.13e-17 85.9
MS.gene015994.t1 AT2G17700 23.906 297 202 11 20 311 265 542 3.29e-17 85.5
MS.gene015994.t1 AT1G05100 31.492 181 107 7 127 301 89 258 4.86e-16 80.5
MS.gene015994.t1 AT3G63260 25.000 192 136 3 116 305 178 363 1.66e-15 79.3
MS.gene015994.t1 AT3G01490 22.442 303 213 6 19 305 87 383 3.50e-15 78.6
MS.gene015994.t1 AT4G26890 27.568 185 120 4 120 302 77 249 2.23e-14 76.3
MS.gene015994.t1 AT5G01850 26.866 201 132 5 118 311 90 282 3.06e-14 74.7
MS.gene015994.t1 AT5G01850 26.866 201 132 5 118 311 114 306 3.59e-14 74.7
MS.gene015994.t1 AT3G46930 25.628 199 139 4 119 315 275 466 5.15e-14 75.5
MS.gene015994.t1 AT3G46930 25.628 199 139 4 119 315 240 431 5.25e-14 75.1
MS.gene015994.t1 AT4G38470 25.475 263 172 10 8 265 257 500 6.88e-14 75.1
MS.gene015994.t1 AT1G62400 25.758 198 138 4 119 315 161 350 1.01e-13 73.9
MS.gene015994.t1 AT1G08720 26.408 284 183 9 33 311 665 927 1.22e-13 75.1
MS.gene015994.t1 AT5G11850 24.818 274 184 7 38 306 607 863 2.51e-13 73.9
MS.gene015994.t1 AT1G73660 24.088 274 186 7 38 306 746 1002 4.18e-13 73.2
MS.gene015994.t1 AT1G32320 26.119 268 164 11 46 302 54 298 1.10e-12 69.7
MS.gene015994.t1 AT5G58950 24.623 199 142 3 119 316 284 475 1.15e-12 71.2
MS.gene015994.t1 AT1G18160 24.088 274 186 7 38 306 713 969 2.55e-12 70.9
MS.gene015994.t1 AT2G42640 28.022 182 119 6 124 305 567 736 2.66e-12 70.5
MS.gene015994.t1 AT3G45790 28.139 231 144 6 30 250 92 310 2.86e-12 69.3
MS.gene015994.t1 AT2G42640 28.022 182 119 6 124 305 605 774 3.93e-12 70.1
MS.gene015994.t1 AT5G03730 25.714 280 181 11 33 305 547 806 9.63e-12 68.6
MS.gene015994.t1 AT5G03730 25.714 280 181 11 33 305 547 806 9.63e-12 68.6
MS.gene015994.t1 AT3G46140 27.468 233 147 6 28 250 90 310 1.07e-11 67.4
MS.gene015994.t1 AT4G23050 28.662 157 104 4 147 301 567 717 1.48e-11 68.2
MS.gene015994.t1 AT4G23050 28.662 157 104 4 147 301 566 716 1.56e-11 67.8
MS.gene015994.t1 AT4G29810 26.866 201 126 8 115 304 147 337 2.23e-11 66.2
MS.gene015994.t1 AT4G29810 26.633 199 125 8 115 302 138 326 6.37e-11 65.1
MS.gene015994.t1 AT4G29810 26.633 199 125 8 115 302 147 335 7.31e-11 64.7

Find 178 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCATGAGTTGGGAGGCTTTC+TGG 0.031214 1.4:+8480303 None:intergenic
TGACAACGATCAAGCGATTT+TGG 0.146585 1.4:-8481325 MS.gene015994:CDS
GATTCGAATGGCGTCGTAAA+AGG 0.159414 1.4:-8479922 MS.gene015994:CDS
GAAGAACATTACATGGTTTA+AGG 0.171246 1.4:+8481352 None:intergenic
AGAGCTTCTGTTGGATTTAT+TGG 0.180068 1.4:-8480046 MS.gene015994:CDS
ACCTCTGGTTTCCATTGTTC+TGG 0.192206 1.4:+8481213 None:intergenic
CATGGAATTTCAATGTTAAA+TGG 0.209362 1.4:-8481725 MS.gene015994:CDS
GTTAAGCACCATCTTTAAAA+AGG 0.241242 1.4:+8479504 None:intergenic
TGTTCTGGAGCCATGTAATT+AGG 0.250797 1.4:+8481228 None:intergenic
GAGCTTCTGTTGGATTTATT+GGG 0.274049 1.4:-8480045 MS.gene015994:CDS
TGCTGAAGTTTCAAAGGTTC+AGG 0.298858 1.4:+8481941 None:intergenic
TTAGAACGTACTGCAGATTA+TGG 0.302417 1.4:-8480238 MS.gene015994:CDS
GGGCTTAACACATTCTCTTT+TGG 0.303938 1.4:+8479947 None:intergenic
TTCTAGAGCTTCACGCTAAA+GGG 0.305443 1.4:-8481389 MS.gene015994:CDS
TTTCCAGGGATGGTGAGTTT+TGG 0.309534 1.4:-8479836 MS.gene015994:CDS
ATATTCTGCGCGTCTTTAAA+AGG 0.310548 1.4:-8480963 MS.gene015994:intron
GTCGATGTATGAATTCTTAT+AGG 0.311891 1.4:+8480181 None:intergenic
TGTTTGGTTGTCAAGTTTCC+AGG 0.313479 1.4:-8479851 MS.gene015994:CDS
TAGTCAGTGCTGCCTGATTT+TGG 0.315215 1.4:+8480379 None:intergenic
TTCACATGATCCTCTTTGAT+TGG 0.322968 1.4:+8481815 None:intergenic
TCTGCGTGGGCCAGTTTGTT+AGG 0.324940 1.4:-8479970 MS.gene015994:CDS
CTGCTACTGGTAGGATTTCA+TGG 0.337558 1.4:-8479889 MS.gene015994:CDS
GAGGACCCTTTGGTGATGTT+TGG 0.361104 1.4:-8481907 MS.gene015994:CDS
CCATGGGTTATAATCCTTGT+AGG 0.362031 1.4:+8479563 None:intergenic
AAGGAGTTTCTAGTGTTTGC+TGG 0.368878 1.4:-8481751 MS.gene015994:CDS
TGTCCTGTTGGTGAAATATA+TGG 0.373043 1.4:-8481102 MS.gene015994:CDS
ACATCTTAGCAGCAACTTCA+TGG 0.384443 1.4:+8481843 None:intergenic
TTGAAACTTCAGCACCGAAT+TGG 0.395091 1.4:-8481932 MS.gene015994:CDS
GAAACGGATTCATGGGGATT+CGG 0.395952 1.4:-8481171 MS.gene015994:CDS
ATTCGAATGGCGTCGTAAAA+GGG 0.398237 1.4:-8479921 MS.gene015994:CDS
CCTCTCAAAGGATCAACTTC+AGG 0.398972 1.4:-8480344 MS.gene015994:CDS
TTGAAACTAGGCATATTAGT+TGG 0.401044 1.4:-8479665 MS.gene015994:CDS
TCGATGTATGAATTCTTATA+GGG 0.403661 1.4:+8480182 None:intergenic
CGAATTGGTCGAGGACCCTT+TGG 0.406343 1.4:-8481917 MS.gene015994:CDS
TCTACCGTCATTGCGATTGA+TGG 0.408374 1.4:+8480066 None:intergenic
ATATTTACAGCTTTGAAACT+AGG 0.412019 1.4:-8479677 MS.gene015994:CDS
TGAAGAGCCAAGCAAAAGAT+TGG 0.413672 1.4:+8481287 None:intergenic
ATGAAAGTAATTCCAAAATC+AGG 0.416519 1.4:-8480391 MS.gene015994:CDS
ATGGCCTGGCAGCTGGTGAT+TGG 0.421439 1.4:-8480141 MS.gene015994:CDS
TTATTGGGCTGCAAACTCTC+TGG 0.426673 1.4:-8480030 MS.gene015994:CDS
AGGGGAGGGGCCTCTGCTAC+TGG 0.429138 1.4:-8479902 MS.gene015994:CDS
TTGACAACCAAACACCCATT+TGG 0.430784 1.4:+8479860 None:intergenic
ATGCCTAGTACAACTAGAAC+AGG 0.431258 1.4:+8479698 None:intergenic
TGTCATTTGAAACGGATTCA+TGG 0.434134 1.4:-8481179 MS.gene015994:CDS
GGAGGAGGATGGAGATATCA+AGG 0.438936 1.4:-8480418 MS.gene015994:CDS
ATTCTAGAGCTTCACGCTAA+AGG 0.446245 1.4:-8481390 MS.gene015994:CDS
GATACTACCAAATGGGTGTT+TGG 0.448805 1.4:-8479867 MS.gene015994:CDS
TTCCCAAAACTCACCATCCC+TGG 0.449001 1.4:+8479833 None:intergenic
GTCATTTGAAACGGATTCAT+GGG 0.449610 1.4:-8481178 MS.gene015994:CDS
TTTGAGGAATCGACCTTCAA+TGG 0.455368 1.4:-8480989 MS.gene015994:CDS
GTCACGAATGGCCTGGCAGC+TGG 0.457715 1.4:-8480148 MS.gene015994:CDS
TCTAGTGTTTGCTGGTTACA+TGG 0.459137 1.4:-8481743 MS.gene015994:CDS
TCTCCCATTGAGAATATCCT+TGG 0.462519 1.4:-8481030 MS.gene015994:CDS
CGACTAATCGCGCAAGTCCA+TGG 0.464799 1.4:-8481970 MS.gene015994:CDS
CAAAGGGTCCTCGACCAATT+CGG 0.467685 1.4:+8481918 None:intergenic
CATGGACTTGCGCGATTAGT+CGG 0.470794 1.4:+8481971 None:intergenic
ATTTCATGGATACTACCAAA+TGG 0.474128 1.4:-8479875 MS.gene015994:CDS
GACATAGGGCCTCTGACCTC+TGG 0.481700 1.4:+8481198 None:intergenic
GGTTGTCAAGTTTCCAGGGA+TGG 0.483297 1.4:-8479846 MS.gene015994:CDS
GATCCATATATTTCACCAAC+AGG 0.486520 1.4:+8481099 None:intergenic
AAAGGTTCAGGTTCAATCCA+TGG 0.486677 1.4:+8481953 None:intergenic
AGTTGGGAGGCTTTCTGGAT+CGG 0.491406 1.4:+8480308 None:intergenic
AAGCCAACGACAGTTCCGTC+TGG 0.492923 1.4:+8480259 None:intergenic
AGGCCCTATGTCATTTGAAA+CGG 0.494592 1.4:-8481187 MS.gene015994:CDS
GAAGAGCCAAGCAAAAGATT+GGG 0.495194 1.4:+8481288 None:intergenic
AAACCCTTCAGAACTCGAAA+TGG 0.495513 1.4:-8480002 MS.gene015994:CDS
AAATTGTATGACGGTTCAAT+TGG 0.497991 1.4:-8481588 MS.gene015994:CDS
CTGAATGAACTGGCTAATGA+TGG 0.498348 1.4:-8480439 MS.gene015994:CDS
GGTACGCTTGAAAGATGAAA+AGG 0.498765 1.4:-8480119 MS.gene015994:CDS
AGACCTGTTCTAGTTGTACT+AGG 0.500576 1.4:-8479701 MS.gene015994:CDS
TAACCCATGGCAACACAACA+TGG 0.500752 1.4:-8479550 MS.gene015994:CDS
GAGGGGCCTCTGCTACTGGT+AGG 0.503223 1.4:-8479898 MS.gene015994:CDS
TGGCCTGGCAGCTGGTGATT+GGG 0.503490 1.4:-8480140 MS.gene015994:CDS
ATGGGAGAAGGAAGGCCACT+TGG 0.504464 1.4:+8481045 None:intergenic
AACTTCAACCTCGGAAGGAT+CGG 0.506263 1.4:+8479793 None:intergenic
TGCACCAAGGATATTCTCAA+TGG 0.506987 1.4:+8481026 None:intergenic
CGCAGAATATCTACCATTGA+AGG 0.507993 1.4:+8480976 None:intergenic
GAATTTCAATGTTAAATGGA+AGG 0.512178 1.4:-8481721 MS.gene015994:CDS
CTCAAAGGATCAACTTCAGG+TGG 0.513886 1.4:-8480341 MS.gene015994:CDS
TTATGGATTTGTTCTAGTGA+GGG 0.514209 1.4:-8480221 MS.gene015994:CDS
TCAGATATTGCTCAGAGGCT+TGG 0.514336 1.4:-8481255 MS.gene015994:CDS
GTTTGGTTGTCAAGTTTCCA+GGG 0.514420 1.4:-8479850 MS.gene015994:CDS
ACACATCACCTCAAAACATC+AGG 0.516904 1.4:+8481525 None:intergenic
AATTTCAATGTTAAATGGAA+GGG 0.525630 1.4:-8481720 MS.gene015994:intron
TTGCAGGTCACTGAATGAAC+TGG 0.528545 1.4:-8480449 MS.gene015994:intron
TCTGCCATTTCGAGTTCTGA+AGG 0.531466 1.4:+8479998 None:intergenic
GTACCCAATCACCAGCTGCC+AGG 0.532200 1.4:+8480137 None:intergenic
TTTCATGGATACTACCAAAT+GGG 0.532985 1.4:-8479874 MS.gene015994:CDS
AGCAAACACTAGAAACTCCT+TGG 0.533146 1.4:+8481753 None:intergenic
ATGGTTTAAGGTTGAGAACA+AGG 0.536528 1.4:+8481364 None:intergenic
TGAACGCGTCACGAATGGCC+TGG 0.537908 1.4:-8480155 MS.gene015994:CDS
CACCGAGTGGTTCCTCTCAA+AGG 0.541938 1.4:-8480356 MS.gene015994:CDS
CCTTGGTATGGATGTCCTGT+TGG 0.543914 1.4:-8481114 MS.gene015994:CDS
ATTATGGATTTGTTCTAGTG+AGG 0.545782 1.4:-8480222 MS.gene015994:CDS
TGGCAGGTATGGGATCGATC+TGG 0.548820 1.4:-8481421 MS.gene015994:intron
ATTCGGTGCTGAAGTTTCAA+AGG 0.549441 1.4:+8481935 None:intergenic
TACGACGCCATTCGAATCGA+GGG 0.550381 1.4:+8479927 None:intergenic
GAATCCGTTTCAAATGACAT+AGG 0.550959 1.4:+8481183 None:intergenic
TCATTGAGAAGAACATTACA+TGG 0.551201 1.4:+8481345 None:intergenic
GCACCAAGGATATTCTCAAT+GGG 0.552742 1.4:+8481027 None:intergenic
TTCGAATGGCGTCGTAAAAG+GGG 0.556323 1.4:-8479920 MS.gene015994:CDS
TAGTTGGAAACAAAGTGAAA+AGG 0.558036 1.4:-8479649 MS.gene015994:CDS
AACATGTTGAGGATCATCAC+TGG 0.559885 1.4:-8479730 MS.gene015994:CDS
AAAATCAACAACTTCAACCT+CGG 0.561609 1.4:+8479784 None:intergenic
ACTGGTAGTCAACCTTGGTA+TGG 0.562801 1.4:-8481126 MS.gene015994:CDS
AAAAGGATATTAGCAACTGA+AGG 0.563136 1.4:+8479521 None:intergenic
AACCCATGGCAACACAACAT+GGG 0.563805 1.4:-8479549 MS.gene015994:CDS
CTGCCATTTCGAGTTCTGAA+GGG 0.564065 1.4:+8479999 None:intergenic
AGCCAACGACAGTTCCGTCT+GGG 0.566135 1.4:+8480260 None:intergenic
GGGCTGCAAACTCTCTGGAA+TGG 0.566139 1.4:-8480025 MS.gene015994:CDS
ACTCTTGAACGCGTCACGAA+TGG 0.567776 1.4:-8480160 MS.gene015994:CDS
TGAGCTTTGCATGAGTTGGG+AGG 0.571011 1.4:+8480295 None:intergenic
TGGCTAATGATGGAGGAGGA+TGG 0.574319 1.4:-8480429 MS.gene015994:CDS
ACCCATGGCAACACAACATG+GGG 0.575393 1.4:-8479548 MS.gene015994:CDS
ATTACATGGCTCCAGAACAA+TGG 0.575533 1.4:-8481224 MS.gene015994:CDS
TCAACTTCAGGTGGGTGACA+TGG 0.577067 1.4:-8480332 MS.gene015994:CDS
AGAAAAGTATCAACATGTTG+AGG 0.578832 1.4:-8479741 MS.gene015994:CDS
TGCAAGCCAAACATCACCAA+AGG 0.581260 1.4:+8481901 None:intergenic
AGGATATTCTCAATGGGAGA+AGG 0.581766 1.4:+8481033 None:intergenic
TTTGTTCTAGTGAGGGTCCA+CGG 0.582005 1.4:-8480214 MS.gene015994:CDS
TGGGAATGAATCAAACACCT+TGG 0.582420 1.4:-8479816 MS.gene015994:CDS
TCAACAACTTCAACCTCGGA+AGG 0.582617 1.4:+8479788 None:intergenic
CATTCCATCAATCGCAATGA+CGG 0.585990 1.4:-8480070 MS.gene015994:CDS
TGGGAGAAGGAAGGCCACTT+GGG 0.586231 1.4:+8481046 None:intergenic
CGGTTCAATTGGTGACAAGT+TGG 0.586939 1.4:-8481577 MS.gene015994:CDS
AATCCGTTTCAAATGACATA+GGG 0.587008 1.4:+8481184 None:intergenic
AAGGAAAAGCACTCACCGGT+GGG 0.588053 1.4:-8480100 MS.gene015994:CDS
AAAACACAAACAGTCCTACA+AGG 0.589182 1.4:-8479577 MS.gene015994:CDS
ACAAAGTGAAAAGGTGCAAG+AGG 0.591652 1.4:-8479640 MS.gene015994:CDS
CACCTTGGCCGATCCTTCCG+AGG 0.592446 1.4:-8479801 MS.gene015994:CDS
AACCTCGGAAGGATCGGCCA+AGG 0.594129 1.4:+8479799 None:intergenic
TGGCTAGACTAAGAGATGGA+TGG 0.594983 1.4:-8481557 MS.gene015994:CDS
GATGTTACATCCAATCAAAG+AGG 0.595757 1.4:-8481825 MS.gene015994:CDS
TTACGACGCCATTCGAATCG+AGG 0.595769 1.4:+8479926 None:intergenic
GCAAGCCAAACATCACCAAA+GGG 0.597883 1.4:+8481902 None:intergenic
TATGGGATCGATCTGGCTCA+AGG 0.601763 1.4:-8481414 MS.gene015994:CDS
GAATTATGCTTGAAGTGCCA+AGG 0.602260 1.4:-8481770 MS.gene015994:CDS
CTTCAGCACCGAATTGGTCG+AGG 0.602330 1.4:-8481926 MS.gene015994:CDS
GCTTGGAAGTCCTAATTACA+TGG 0.604254 1.4:-8481238 MS.gene015994:CDS
ATCATTATGAAATTGTATGA+CGG 0.604681 1.4:-8481597 MS.gene015994:CDS
TGTTGACTGGTAGTCAACCT+TGG 0.606529 1.4:-8481131 MS.gene015994:CDS
ACCGTTGTTGAAATGTTGAC+TGG 0.606786 1.4:-8481144 MS.gene015994:CDS
CCTGAAGTTGATCCTTTGAG+AGG 0.610469 1.4:+8480344 None:intergenic
AATGGCGTCGTAAAAGGGGA+GGG 0.612881 1.4:-8479916 MS.gene015994:CDS
GTCCCAGACGGAACTGTCGT+TGG 0.613432 1.4:-8480262 MS.gene015994:CDS
TCTAGAGCTTCACGCTAAAG+GGG 0.615277 1.4:-8481388 MS.gene015994:CDS
TGTTAAGCCCTCGATTCGAA+TGG 0.618387 1.4:-8479934 MS.gene015994:CDS
TCATTTGAAACGGATTCATG+GGG 0.622874 1.4:-8481177 MS.gene015994:CDS
TGAAAAGGAAAAGCACTCAC+CGG 0.625000 1.4:-8480104 MS.gene015994:CDS
ATGGCAGAATCATTCTGCGT+GGG 0.627438 1.4:-8479983 MS.gene015994:CDS
AAGTTGGCTAGACTAAGAGA+TGG 0.628842 1.4:-8481561 MS.gene015994:CDS
TGAAATCCTACCAGTAGCAG+AGG 0.629390 1.4:+8479892 None:intergenic
AAAGGAAAAGCACTCACCGG+TGG 0.630780 1.4:-8480101 MS.gene015994:CDS
ATCCTTTGAGAGGAACCACT+CGG 0.632608 1.4:+8480354 None:intergenic
ATGGCGTCGTAAAAGGGGAG+GGG 0.637421 1.4:-8479915 MS.gene015994:CDS
GAATGGCGTCGTAAAAGGGG+AGG 0.641779 1.4:-8479917 MS.gene015994:CDS
TATTCTCAATGGGAGAAGGA+AGG 0.642358 1.4:+8481037 None:intergenic
AATGACGGTAGAGCTTCTGT+TGG 0.643785 1.4:-8480055 MS.gene015994:CDS
CAATGGAAACCAGAGGTCAG+AGG 0.644185 1.4:-8481207 MS.gene015994:CDS
ACATGTTGAGGATCATCACT+GGG 0.644328 1.4:-8479729 MS.gene015994:CDS
TCAAAGGATCAACTTCAGGT+GGG 0.652726 1.4:-8480340 MS.gene015994:CDS
ATGGAATGTAGAATCCCCAC+CGG 0.657028 1.4:+8480085 None:intergenic
GAAAAGCTGCATATCCCAAG+TGG 0.662621 1.4:-8481060 MS.gene015994:CDS
ACCAGTCAACATTTCAACAA+CGG 0.664981 1.4:+8481143 None:intergenic
CCTACAAGGATTATAACCCA+TGG 0.667895 1.4:-8479563 MS.gene015994:CDS
AATGGCAGAATCATTCTGCG+TGG 0.675086 1.4:-8479984 MS.gene015994:CDS
AATGAACTGGCTAATGATGG+AGG 0.681313 1.4:-8480436 MS.gene015994:CDS
CAAAACATGAATGTCCCAGA+CGG 0.685960 1.4:-8480274 MS.gene015994:CDS
CCATGGCAACACAACATGGG+GGG 0.686405 1.4:-8479546 MS.gene015994:CDS
TTAGCTCAGATATTGCTCAG+AGG 0.694542 1.4:-8481260 MS.gene015994:CDS
AGGAAAAGCACTCACCGGTG+GGG 0.698636 1.4:-8480099 MS.gene015994:CDS
GAACTGGCTAATGATGGAGG+AGG 0.699870 1.4:-8480433 MS.gene015994:CDS
TCCAGAACAATGGAAACCAG+AGG 0.702393 1.4:-8481214 MS.gene015994:CDS
CATACTCAAAACATGCACCA+AGG 0.704149 1.4:+8481013 None:intergenic
CCCATGGCAACACAACATGG+GGG 0.707761 1.4:-8479547 MS.gene015994:CDS
TTATAGGGTCATGAATACCG+TGG 0.720576 1.4:+8480197 None:intergenic
CCAACAGGACATCCATACCA+AGG 0.725485 1.4:+8481114 None:intergenic
GCAGCACTGACTACACCGAG+TGG 0.764598 1.4:-8480369 MS.gene015994:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTCAATGTTAAATGGAA+GGG - chr1.4:8479834-8479853 MS.gene015994:CDS 20.0%
!! ATCATTATGAAATTGTATGA+CGG - chr1.4:8479957-8479976 MS.gene015994:CDS 20.0%
!! ATGCTAAATTAAACAAGAAT+CGG + chr1.4:8480664-8480683 None:intergenic 20.0%
!!! ATCTTTAACTTATATAGCAT+AGG - chr1.4:8480070-8480089 MS.gene015994:CDS 20.0%
!!! TCTTTAACTTATATAGCATA+GGG - chr1.4:8480071-8480090 MS.gene015994:CDS 20.0%
!!! TTCTTTTGATTATGAGATTT+TGG - chr1.4:8479525-8479544 MS.gene015994:CDS 20.0%
! AAAATTACTGGTCAATACTA+AGG - chr1.4:8480094-8480113 MS.gene015994:CDS 25.0%
! ATGAAAGTAATTCCAAAATC+AGG - chr1.4:8481163-8481182 MS.gene015994:CDS 25.0%
! CATGGAATTTCAATGTTAAA+TGG - chr1.4:8479829-8479848 MS.gene015994:CDS 25.0%
! GAATTTCAATGTTAAATGGA+AGG - chr1.4:8479833-8479852 MS.gene015994:CDS 25.0%
! GTGCATTTATTTCAATGTAT+AGG - chr1.4:8479870-8479889 MS.gene015994:CDS 25.0%
! TCGATGTATGAATTCTTATA+GGG + chr1.4:8481375-8481394 None:intergenic 25.0%
! TTTCAATTATCACTTCACAT+TGG - chr1.4:8480695-8480714 MS.gene015994:intron 25.0%
!!! ATATTTACAGCTTTGAAACT+AGG - chr1.4:8481877-8481896 MS.gene015994:CDS 25.0%
!!! CTTATTGATTTAGTTCTTTC+AGG + chr1.4:8480968-8480987 None:intergenic 25.0%
!!! GCTAATATCCTTTTTAAAGA+TGG - chr1.4:8482042-8482061 MS.gene015994:CDS 25.0%
AAAAGGATATTAGCAACTGA+AGG + chr1.4:8482036-8482055 None:intergenic 30.0%
AAAATCAACAACTTCAACCT+CGG + chr1.4:8481773-8481792 None:intergenic 30.0%
AAATTGTATGACGGTTCAAT+TGG - chr1.4:8479966-8479985 MS.gene015994:CDS 30.0%
AATCCGTTTCAAATGACATA+GGG + chr1.4:8480373-8480392 None:intergenic 30.0%
ATTCCTGTGATGTATTATCT+TGG - chr1.4:8480896-8480915 MS.gene015994:intron 30.0%
ATTTCATGGATACTACCAAA+TGG - chr1.4:8481679-8481698 MS.gene015994:intron 30.0%
CGGCATCAGAAAAAAAAAAA+TGG + chr1.4:8480644-8480663 None:intergenic 30.0%
GAAGAACATTACATGGTTTA+AGG + chr1.4:8480205-8480224 None:intergenic 30.0%
GTCGATGTATGAATTCTTAT+AGG + chr1.4:8481376-8481395 None:intergenic 30.0%
TAGCTTGATAGAAGTCAAAA+GGG + chr1.4:8479909-8479928 None:intergenic 30.0%
TAGTTGGAAACAAAGTGAAA+AGG - chr1.4:8481905-8481924 MS.gene015994:CDS 30.0%
TATAGCATAGGGAAAATTAC+TGG - chr1.4:8480082-8480101 MS.gene015994:CDS 30.0%
TCATTGAGAAGAACATTACA+TGG + chr1.4:8480212-8480231 None:intergenic 30.0%
TTAAATGGAAGGGTTAGATT+TGG - chr1.4:8479844-8479863 MS.gene015994:CDS 30.0%
TTATGATCATGACCGATTAT+TGG - chr1.4:8480778-8480797 MS.gene015994:intron 30.0%
TTCCTGTGATGTATTATCTT+GGG - chr1.4:8480897-8480916 MS.gene015994:intron 30.0%
TTGAAACTAGGCATATTAGT+TGG - chr1.4:8481889-8481908 MS.gene015994:CDS 30.0%
TTTCATGGATACTACCAAAT+GGG - chr1.4:8481680-8481699 MS.gene015994:intron 30.0%
! ATAGTGGTTTTTGCTTTCTT+TGG + chr1.4:8481947-8481966 None:intergenic 30.0%
! ATTATGGATTTGTTCTAGTG+AGG - chr1.4:8481332-8481351 MS.gene015994:CDS 30.0%
! TTAGCTTGATAGAAGTCAAA+AGG + chr1.4:8479910-8479929 None:intergenic 30.0%
! TTATGGATTTGTTCTAGTGA+GGG - chr1.4:8481333-8481352 MS.gene015994:CDS 30.0%
!! AGAAAAGTATCAACATGTTG+AGG - chr1.4:8481813-8481832 MS.gene015994:CDS 30.0%
!! CATGTTTTGAGTATGATTTG+AGG - chr1.4:8480549-8480568 MS.gene015994:intron 30.0%
!! TTGATTTTAACACTTGTCCT+GGG - chr1.4:8481785-8481804 MS.gene015994:CDS 30.0%
AAAACACAAACAGTCCTACA+AGG - chr1.4:8481977-8481996 MS.gene015994:CDS 35.0%
ACCAGTCAACATTTCAACAA+CGG + chr1.4:8480414-8480433 None:intergenic 35.0%
AGAGCTTCTGTTGGATTTAT+TGG - chr1.4:8481508-8481527 MS.gene015994:intron 35.0%
AGCAAAAACCACTATGTTGA+AGG - chr1.4:8481952-8481971 MS.gene015994:CDS 35.0%
AGGAATACAGCTAATCTAGA+AGG + chr1.4:8480882-8480901 None:intergenic 35.0%
ATATTCTGCGCGTCTTTAAA+AGG - chr1.4:8480591-8480610 MS.gene015994:intron 35.0%
CCTGTGAATATTCCAAGATT+GGG - chr1.4:8480816-8480835 MS.gene015994:intron 35.0%
GAATCCGTTTCAAATGACAT+AGG + chr1.4:8480374-8480393 None:intergenic 35.0%
GAGCTTCTGTTGGATTTATT+GGG - chr1.4:8481509-8481528 MS.gene015994:intron 35.0%
GATCCATATATTTCACCAAC+AGG + chr1.4:8480458-8480477 None:intergenic 35.0%
GATGTTACATCCAATCAAAG+AGG - chr1.4:8479729-8479748 MS.gene015994:CDS 35.0%
TCCTGTGAATATTCCAAGAT+TGG - chr1.4:8480815-8480834 MS.gene015994:intron 35.0%
TGAATATTCCAAGATTGGGT+AGG - chr1.4:8480820-8480839 MS.gene015994:intron 35.0%
TTAGAACGTACTGCAGATTA+TGG - chr1.4:8481316-8481335 MS.gene015994:CDS 35.0%
TTCACATGATCCTCTTTGAT+TGG + chr1.4:8479742-8479761 None:intergenic 35.0%
TTCCCAAGATAATACATCAC+AGG + chr1.4:8480902-8480921 None:intergenic 35.0%
! AAGGATGTGTTGGTCATTAA+TGG - chr1.4:8480113-8480132 MS.gene015994:CDS 35.0%
! ATGGTTTAAGGTTGAGAACA+AGG + chr1.4:8480193-8480212 None:intergenic 35.0%
! GTCATTTGAAACGGATTCAT+GGG - chr1.4:8480376-8480395 MS.gene015994:CDS 35.0%
! TCATTTGAAACGGATTCATG+GGG - chr1.4:8480377-8480396 MS.gene015994:CDS 35.0%
! TCTCATTACCTGATGTTTTG+AGG - chr1.4:8480021-8480040 MS.gene015994:CDS 35.0%
! TGTCATTTGAAACGGATTCA+TGG - chr1.4:8480375-8480394 MS.gene015994:CDS 35.0%
! TGTCCTGTTGGTGAAATATA+TGG - chr1.4:8480452-8480471 MS.gene015994:intron 35.0%
! TTTTGAGCTTTGCATGAGTT+GGG + chr1.4:8481265-8481284 None:intergenic 35.0%
!! GTTGATTTTAACACTTGTCC+TGG - chr1.4:8481784-8481803 MS.gene015994:CDS 35.0%
!!! CTTGGGAAGTGTTTACATTT+TGG - chr1.4:8480914-8480933 MS.gene015994:intron 35.0%
!!! TGTTTTGACCTTCAACATAG+TGG + chr1.4:8481963-8481982 None:intergenic 35.0%
AAACCCTTCAGAACTCGAAA+TGG - chr1.4:8481552-8481571 MS.gene015994:CDS 40.0%
AACATGTTGAGGATCATCAC+TGG - chr1.4:8481824-8481843 MS.gene015994:CDS 40.0%
AAGTTGGCTAGACTAAGAGA+TGG - chr1.4:8479993-8480012 MS.gene015994:CDS 40.0%
AATATTCACAGGACAGGTAC+AGG + chr1.4:8480808-8480827 None:intergenic 40.0%
AATGAACTGGCTAATGATGG+AGG - chr1.4:8481118-8481137 MS.gene015994:CDS 40.0%
ACACATCACCTCAAAACATC+AGG + chr1.4:8480032-8480051 None:intergenic 40.0%
ACATCTTAGCAGCAACTTCA+TGG + chr1.4:8479714-8479733 None:intergenic 40.0%
ACATGTTGAGGATCATCACT+GGG - chr1.4:8481825-8481844 MS.gene015994:CDS 40.0%
ACCGTTGTTGAAATGTTGAC+TGG - chr1.4:8480410-8480429 MS.gene015994:CDS 40.0%
AGCAAACACTAGAAACTCCT+TGG + chr1.4:8479804-8479823 None:intergenic 40.0%
AGGATATTCTCAATGGGAGA+AGG + chr1.4:8480524-8480543 None:intergenic 40.0%
ATGCCTAGTACAACTAGAAC+AGG + chr1.4:8481859-8481878 None:intergenic 40.0%
ATTACATGGCTCCAGAACAA+TGG - chr1.4:8480330-8480349 MS.gene015994:CDS 40.0%
ATTCATTCTGCCTATGTTGC+AGG - chr1.4:8481089-8481108 MS.gene015994:CDS 40.0%
ATTCGAATGGCGTCGTAAAA+GGG - chr1.4:8481633-8481652 MS.gene015994:intron 40.0%
CAAAACATGAATGTCCCAGA+CGG - chr1.4:8481280-8481299 MS.gene015994:CDS 40.0%
CATACTCAAAACATGCACCA+AGG + chr1.4:8480544-8480563 None:intergenic 40.0%
CATTCCATCAATCGCAATGA+CGG - chr1.4:8481484-8481503 MS.gene015994:intron 40.0%
CCCAATCTTGGAATATTCAC+AGG + chr1.4:8480819-8480838 None:intergenic 40.0%
CCTACAAGGATTATAACCCA+TGG - chr1.4:8481991-8482010 MS.gene015994:CDS 40.0%
CGCAGAATATCTACCATTGA+AGG + chr1.4:8480581-8480600 None:intergenic 40.0%
CTGAATGAACTGGCTAATGA+TGG - chr1.4:8481115-8481134 MS.gene015994:CDS 40.0%
GAAGAGCCAAGCAAAAGATT+GGG + chr1.4:8480269-8480288 None:intergenic 40.0%
GATACTACCAAATGGGTGTT+TGG - chr1.4:8481687-8481706 MS.gene015994:intron 40.0%
GCACCAAGGATATTCTCAAT+GGG + chr1.4:8480530-8480549 None:intergenic 40.0%
GCTTGGAAGTCCTAATTACA+TGG - chr1.4:8480316-8480335 MS.gene015994:CDS 40.0%
GGTACAGGTTCACCAATAAT+CGG + chr1.4:8480793-8480812 None:intergenic 40.0%
GGTACGCTTGAAAGATGAAA+AGG - chr1.4:8481435-8481454 MS.gene015994:intron 40.0%
GGTCAATACTAAGGATGTGT+TGG - chr1.4:8480103-8480122 MS.gene015994:CDS 40.0%
GTTTGGTTGTCAAGTTTCCA+GGG - chr1.4:8481704-8481723 MS.gene015994:intron 40.0%
TATTCTCAATGGGAGAAGGA+AGG + chr1.4:8480520-8480539 None:intergenic 40.0%
TCAAAGGATCAACTTCAGGT+GGG - chr1.4:8481214-8481233 MS.gene015994:CDS 40.0%
TCTCCCATTGAGAATATCCT+TGG - chr1.4:8480524-8480543 MS.gene015994:intron 40.0%
TCTTGGAATATTCACAGGAC+AGG + chr1.4:8480814-8480833 None:intergenic 40.0%
TGAAGAGCCAAGCAAAAGAT+TGG + chr1.4:8480270-8480289 None:intergenic 40.0%
TGCACCAAGGATATTCTCAA+TGG + chr1.4:8480531-8480550 None:intergenic 40.0%
TGCTGAAGTTTCAAAGGTTC+AGG + chr1.4:8479616-8479635 None:intergenic 40.0%
TGGGAATGAATCAAACACCT+TGG - chr1.4:8481738-8481757 MS.gene015994:CDS 40.0%
TGGTCATTAATGGCAGGTAT+GGG - chr1.4:8480123-8480142 MS.gene015994:CDS 40.0%
TGTTTGGTTGTCAAGTTTCC+AGG - chr1.4:8481703-8481722 MS.gene015994:intron 40.0%
TTAGCTCAGATATTGCTCAG+AGG - chr1.4:8480294-8480313 MS.gene015994:CDS 40.0%
TTATAGGGTCATGAATACCG+TGG + chr1.4:8481360-8481379 None:intergenic 40.0%
TTCAGGTACAGACATTATCC+TGG + chr1.4:8480951-8480970 None:intergenic 40.0%
TTGACAACCAAACACCCATT+TGG + chr1.4:8481697-8481716 None:intergenic 40.0%
TTGGTCATTAATGGCAGGTA+TGG - chr1.4:8480122-8480141 MS.gene015994:CDS 40.0%
TTTGAGGAATCGACCTTCAA+TGG - chr1.4:8480565-8480584 MS.gene015994:intron 40.0%
! AAAGGTTCAGGTTCAATCCA+TGG + chr1.4:8479604-8479623 None:intergenic 40.0%
! ACAAAGTGAAAAGGTGCAAG+AGG - chr1.4:8481914-8481933 MS.gene015994:CDS 40.0%
! AGACCTGTTCTAGTTGTACT+AGG - chr1.4:8481853-8481872 MS.gene015994:CDS 40.0%
! AGGCCCTATGTCATTTGAAA+CGG - chr1.4:8480367-8480386 MS.gene015994:CDS 40.0%
! ATGTGTTGGTCATTAATGGC+AGG - chr1.4:8480117-8480136 MS.gene015994:CDS 40.0%
! CCATGGGTTATAATCCTTGT+AGG + chr1.4:8481994-8482013 None:intergenic 40.0%
! GAATTATGCTTGAAGTGCCA+AGG - chr1.4:8479784-8479803 MS.gene015994:CDS 40.0%
! GACAACGATCAAGCGATTTT+GGG - chr1.4:8480230-8480249 MS.gene015994:CDS 40.0%
! GGGCTTAACACATTCTCTTT+TGG + chr1.4:8481610-8481629 None:intergenic 40.0%
! GGTATTCCCAATCTTTTGCT+TGG - chr1.4:8480260-8480279 MS.gene015994:CDS 40.0%
! TCTAGTGTTTGCTGGTTACA+TGG - chr1.4:8479811-8479830 MS.gene015994:CDS 40.0%
! TCTCTTTTGGCCTAACAAAC+TGG + chr1.4:8481597-8481616 None:intergenic 40.0%
! TGACAACGATCAAGCGATTT+TGG - chr1.4:8480229-8480248 MS.gene015994:CDS 40.0%
! TGATACTTTTCTACCATCCC+AGG + chr1.4:8481805-8481824 None:intergenic 40.0%
! TTGAAACTTCAGCACCGAAT+TGG - chr1.4:8479622-8479641 MS.gene015994:CDS 40.0%
! TTTTAACACTTGTCCTGGGA+TGG - chr1.4:8481789-8481808 MS.gene015994:CDS 40.0%
!! AAGGAGTTTCTAGTGTTTGC+TGG - chr1.4:8479803-8479822 MS.gene015994:CDS 40.0%
!! ATTCGGTGCTGAAGTTTCAA+AGG + chr1.4:8479622-8479641 None:intergenic 40.0%
!! ATTCTAGAGCTTCACGCTAA+AGG - chr1.4:8480164-8480183 MS.gene015994:CDS 40.0%
!! GTTTTGAGCTTTGCATGAGT+TGG + chr1.4:8481266-8481285 None:intergenic 40.0%
!! TGAAAAGGAAAAGCACTCAC+CGG - chr1.4:8481450-8481469 MS.gene015994:intron 40.0%
!! TGTTCTGGAGCCATGTAATT+AGG + chr1.4:8480329-8480348 None:intergenic 40.0%
!! TTCTAGAGCTTCACGCTAAA+GGG - chr1.4:8480165-8480184 MS.gene015994:CDS 40.0%
AACCCATGGCAACACAACAT+GGG - chr1.4:8482005-8482024 MS.gene015994:CDS 45.0%
AACTTCAACCTCGGAAGGAT+CGG + chr1.4:8481764-8481783 None:intergenic 45.0%
AATGACGGTAGAGCTTCTGT+TGG - chr1.4:8481499-8481518 MS.gene015994:intron 45.0%
AATGGCAGAATCATTCTGCG+TGG - chr1.4:8481570-8481589 MS.gene015994:CDS 45.0%
ACAACAGACCTACCCAATCT+TGG + chr1.4:8480831-8480850 None:intergenic 45.0%
ACTGGTAGTCAACCTTGGTA+TGG - chr1.4:8480428-8480447 MS.gene015994:CDS 45.0%
AGATTGGGTAGGTCTGTTGT+AGG - chr1.4:8480831-8480850 MS.gene015994:intron 45.0%
ATGGAATGTAGAATCCCCAC+CGG + chr1.4:8481472-8481491 None:intergenic 45.0%
ATGGCAGAATCATTCTGCGT+GGG - chr1.4:8481571-8481590 MS.gene015994:CDS 45.0%
CAGACATTATCCTGGATCAG+AGG + chr1.4:8480943-8480962 None:intergenic 45.0%
CATTCAGTGACCTGCAACAT+AGG + chr1.4:8481102-8481121 None:intergenic 45.0%
CCTCTCAAAGGATCAACTTC+AGG - chr1.4:8481210-8481229 MS.gene015994:CDS 45.0%
CTCAAAGGATCAACTTCAGG+TGG - chr1.4:8481213-8481232 MS.gene015994:CDS 45.0%
CTGCTACTGGTAGGATTTCA+TGG - chr1.4:8481665-8481684 MS.gene015994:intron 45.0%
GAAAAGCTGCATATCCCAAG+TGG - chr1.4:8480494-8480513 MS.gene015994:intron 45.0%
GATTCGAATGGCGTCGTAAA+AGG - chr1.4:8481632-8481651 MS.gene015994:intron 45.0%
GCAAGCCAAACATCACCAAA+GGG + chr1.4:8479655-8479674 None:intergenic 45.0%
TAACCCATGGCAACACAACA+TGG - chr1.4:8482004-8482023 MS.gene015994:CDS 45.0%
TCAACAACTTCAACCTCGGA+AGG + chr1.4:8481769-8481788 None:intergenic 45.0%
TCAGATATTGCTCAGAGGCT+TGG - chr1.4:8480299-8480318 MS.gene015994:CDS 45.0%
TCCAGAACAATGGAAACCAG+AGG - chr1.4:8480340-8480359 MS.gene015994:CDS 45.0%
TCTACCGTCATTGCGATTGA+TGG + chr1.4:8481491-8481510 None:intergenic 45.0%
TGAAATCCTACCAGTAGCAG+AGG + chr1.4:8481665-8481684 None:intergenic 45.0%
TGCAAGCCAAACATCACCAA+AGG + chr1.4:8479656-8479675 None:intergenic 45.0%
TGGCTAGACTAAGAGATGGA+TGG - chr1.4:8479997-8480016 MS.gene015994:CDS 45.0%
TTATTGGGCTGCAAACTCTC+TGG - chr1.4:8481524-8481543 MS.gene015994:intron 45.0%
TTCGAATGGCGTCGTAAAAG+GGG - chr1.4:8481634-8481653 MS.gene015994:intron 45.0%
TTGCAGGTCACTGAATGAAC+TGG - chr1.4:8481105-8481124 MS.gene015994:CDS 45.0%
TTTGTTCTAGTGAGGGTCCA+CGG - chr1.4:8481340-8481359 MS.gene015994:CDS 45.0%
! ACCTCTGGTTTCCATTGTTC+TGG + chr1.4:8480344-8480363 None:intergenic 45.0%
! ATCCTTTGAGAGGAACCACT+CGG + chr1.4:8481203-8481222 None:intergenic 45.0%
! CCTGAAGTTGATCCTTTGAG+AGG + chr1.4:8481213-8481232 None:intergenic 45.0%
! CGGTTCAATTGGTGACAAGT+TGG - chr1.4:8479977-8479996 MS.gene015994:CDS 45.0%
! GAAACGGATTCATGGGGATT+CGG - chr1.4:8480383-8480402 MS.gene015994:CDS 45.0%
! TAGTCAGTGCTGCCTGATTT+TGG + chr1.4:8481178-8481197 None:intergenic 45.0%
! TCGGAACAGCAGCAGTTTTA+GGG + chr1.4:8479567-8479586 None:intergenic 45.0%
! TCTAGAGCTTCACGCTAAAG+GGG - chr1.4:8480166-8480185 MS.gene015994:CDS 45.0%
! TGTTAAGCCCTCGATTCGAA+TGG - chr1.4:8481620-8481639 MS.gene015994:intron 45.0%
! TGTTGACTGGTAGTCAACCT+TGG - chr1.4:8480423-8480442 MS.gene015994:CDS 45.0%
! TGTTGTAGGTCTAGAGCTCA+AGG - chr1.4:8480845-8480864 MS.gene015994:intron 45.0%
! TTCCAGGGATGGTGAGTTTT+GGG - chr1.4:8481719-8481738 MS.gene015994:intron 45.0%
! TTTCCAGGGATGGTGAGTTT+TGG - chr1.4:8481718-8481737 MS.gene015994:intron 45.0%
!! CTGCCATTTCGAGTTCTGAA+GGG + chr1.4:8481558-8481577 None:intergenic 45.0%
!! TCTGCCATTTCGAGTTCTGA+AGG + chr1.4:8481559-8481578 None:intergenic 45.0%
!!! ATTTTGGCTTCCTCTGATCC+AGG - chr1.4:8480930-8480949 MS.gene015994:intron 45.0%
AAGCTGTTTGTGCACGAGCA+TGG - chr1.4:8480992-8481011 MS.gene015994:CDS 50.0%
AATGGCGTCGTAAAAGGGGA+GGG - chr1.4:8481638-8481657 MS.gene015994:intron 50.0%
ACCCATGGCAACACAACATG+GGG - chr1.4:8482006-8482025 MS.gene015994:CDS 50.0%
ACTCTTGAACGCGTCACGAA+TGG - chr1.4:8481394-8481413 MS.gene015994:CDS 50.0%
AGTTGGGAGGCTTTCTGGAT+CGG + chr1.4:8481249-8481268 None:intergenic 50.0%
CAAAGGGTCCTCGACCAATT+CGG + chr1.4:8479639-8479658 None:intergenic 50.0%
CAATGGAAACCAGAGGTCAG+AGG - chr1.4:8480347-8480366 MS.gene015994:CDS 50.0%
CATGGACTTGCGCGATTAGT+CGG + chr1.4:8479586-8479605 None:intergenic 50.0%
CCAACAGGACATCCATACCA+AGG + chr1.4:8480443-8480462 None:intergenic 50.0%
CCTTGGTATGGATGTCCTGT+TGG - chr1.4:8480440-8480459 MS.gene015994:CDS 50.0%
GGAGGAGGATGGAGATATCA+AGG - chr1.4:8481136-8481155 MS.gene015994:CDS 50.0%
GGTTGTCAAGTTTCCAGGGA+TGG - chr1.4:8481708-8481727 MS.gene015994:intron 50.0%
TACGACGCCATTCGAATCGA+GGG + chr1.4:8481630-8481649 None:intergenic 50.0%
TCAACTTCAGGTGGGTGACA+TGG - chr1.4:8481222-8481241 MS.gene015994:CDS 50.0%
TTACGACGCCATTCGAATCG+AGG + chr1.4:8481631-8481650 None:intergenic 50.0%
TTCCCAAAACTCACCATCCC+TGG + chr1.4:8481724-8481743 None:intergenic 50.0%
! GAACTGGCTAATGATGGAGG+AGG - chr1.4:8481121-8481140 MS.gene015994:CDS 50.0%
! GTCGGAACAGCAGCAGTTTT+AGG + chr1.4:8479568-8479587 None:intergenic 50.0%
! TATGGGATCGATCTGGCTCA+AGG - chr1.4:8480140-8480159 MS.gene015994:CDS 50.0%
! TGAGCTTTGCATGAGTTGGG+AGG + chr1.4:8481262-8481281 None:intergenic 50.0%
! TGGCTAATGATGGAGGAGGA+TGG - chr1.4:8481125-8481144 MS.gene015994:CDS 50.0%
!! AAAGGAAAAGCACTCACCGG+TGG - chr1.4:8481453-8481472 MS.gene015994:intron 50.0%
!! AAGGAAAAGCACTCACCGGT+GGG - chr1.4:8481454-8481473 MS.gene015994:intron 50.0%
!! CAAGCGATTTTGGGAGACGT+TGG - chr1.4:8480239-8480258 MS.gene015994:CDS 50.0%
!! GAGGACCCTTTGGTGATGTT+TGG - chr1.4:8479647-8479666 MS.gene015994:CDS 50.0%
!!! AGCAGCAGTTTTAGGGACGT+CGG + chr1.4:8479560-8479579 None:intergenic 50.0%
AAGCCAACGACAGTTCCGTC+TGG + chr1.4:8481298-8481317 None:intergenic 55.0%
AGCCAACGACAGTTCCGTCT+GGG + chr1.4:8481297-8481316 None:intergenic 55.0%
ATGGCGTCGTAAAAGGGGAG+GGG - chr1.4:8481639-8481658 MS.gene015994:intron 55.0%
ATGGGAGAAGGAAGGCCACT+TGG + chr1.4:8480512-8480531 None:intergenic 55.0%
CACCGAGTGGTTCCTCTCAA+AGG - chr1.4:8481198-8481217 MS.gene015994:CDS 55.0%
CCATGGCAACACAACATGGG+GGG - chr1.4:8482008-8482027 MS.gene015994:CDS 55.0%
CCCATGGCAACACAACATGG+GGG - chr1.4:8482007-8482026 MS.gene015994:CDS 55.0%
CCCCCATGTTGTGTTGCCAT+GGG + chr1.4:8482010-8482029 None:intergenic 55.0%
CGAATTGGTCGAGGACCCTT+TGG - chr1.4:8479637-8479656 MS.gene015994:CDS 55.0%
CGACTAATCGCGCAAGTCCA+TGG - chr1.4:8479584-8479603 MS.gene015994:CDS 55.0%
GAATGGCGTCGTAAAAGGGG+AGG - chr1.4:8481637-8481656 MS.gene015994:intron 55.0%
TGGCAGGTATGGGATCGATC+TGG - chr1.4:8480133-8480152 MS.gene015994:CDS 55.0%
TGGGAGAAGGAAGGCCACTT+GGG + chr1.4:8480511-8480530 None:intergenic 55.0%
! CTTCAGCACCGAATTGGTCG+AGG - chr1.4:8479628-8479647 MS.gene015994:CDS 55.0%
! GCATGAGTTGGGAGGCTTTC+TGG + chr1.4:8481254-8481273 None:intergenic 55.0%
! GGGCTGCAAACTCTCTGGAA+TGG - chr1.4:8481529-8481548 MS.gene015994:intron 55.0%
! TCTGCGTGGGCCAGTTTGTT+AGG - chr1.4:8481584-8481603 MS.gene015994:CDS 55.0%
!! AGGAAAAGCACTCACCGGTG+GGG - chr1.4:8481455-8481474 MS.gene015994:intron 55.0%
AACCTCGGAAGGATCGGCCA+AGG + chr1.4:8481758-8481777 None:intergenic 60.0%
ATGGCCTGGCAGCTGGTGAT+TGG - chr1.4:8481413-8481432 MS.gene015994:CDS 60.0%
CCCCCCATGTTGTGTTGCCA+TGG + chr1.4:8482011-8482030 None:intergenic 60.0%
GACATAGGGCCTCTGACCTC+TGG + chr1.4:8480359-8480378 None:intergenic 60.0%
GTACCCAATCACCAGCTGCC+AGG + chr1.4:8481420-8481439 None:intergenic 60.0%
GTCCCAGACGGAACTGTCGT+TGG - chr1.4:8481292-8481311 MS.gene015994:CDS 60.0%
TGAACGCGTCACGAATGGCC+TGG - chr1.4:8481399-8481418 MS.gene015994:CDS 60.0%
TGGCCTGGCAGCTGGTGATT+GGG - chr1.4:8481414-8481433 MS.gene015994:CDS 60.0%
!! GCAGCACTGACTACACCGAG+TGG - chr1.4:8481185-8481204 MS.gene015994:CDS 60.0%
CACCTTGGCCGATCCTTCCG+AGG - chr1.4:8481753-8481772 MS.gene015994:CDS 65.0%
GAGGGGCCTCTGCTACTGGT+AGG - chr1.4:8481656-8481675 MS.gene015994:intron 65.0%
GTCACGAATGGCCTGGCAGC+TGG - chr1.4:8481406-8481425 MS.gene015994:CDS 65.0%
AGGGGAGGGGCCTCTGCTAC+TGG - chr1.4:8481652-8481671 MS.gene015994:intron 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 8479505 8482071 8479505 ID=MS.gene015994
chr1.4 mRNA 8479505 8482071 8479505 ID=MS.gene015994.t1;Parent=MS.gene015994
chr1.4 exon 8481721 8482071 8481721 ID=MS.gene015994.t1.exon1;Parent=MS.gene015994.t1
chr1.4 CDS 8481721 8482071 8481721 ID=cds.MS.gene015994.t1;Parent=MS.gene015994.t1
chr1.4 exon 8481534 8481625 8481534 ID=MS.gene015994.t1.exon2;Parent=MS.gene015994.t1
chr1.4 CDS 8481534 8481625 8481534 ID=cds.MS.gene015994.t1;Parent=MS.gene015994.t1
chr1.4 exon 8480964 8481437 8480964 ID=MS.gene015994.t1.exon3;Parent=MS.gene015994.t1
chr1.4 CDS 8480964 8481437 8480964 ID=cds.MS.gene015994.t1;Parent=MS.gene015994.t1
chr1.4 exon 8479505 8480465 8479505 ID=MS.gene015994.t1.exon4;Parent=MS.gene015994.t1
chr1.4 CDS 8479505 8480465 8479505 ID=cds.MS.gene015994.t1;Parent=MS.gene015994.t1
Gene Sequence

>MS.gene015994

ATGGCTTCTTCATCAGCTGCTTCTTTTGATTATGAGATTTTGGATAAAGATACCGACGTCCCTAAAACTGCTGCTGTTCCGACTAATCGCGCAAGTCCATGGATTGAACCTGAACCTTTGAAACTTCAGCACCGAATTGGTCGAGGACCCTTTGGTGATGTTTGGCTTGCAACTCTTCATCAGTCAACTGAAGATTATGATGAGCACCATGAAGTTGCTGCTAAGATGTTACATCCAATCAAAGAGGATCATGTGAAGATTGTATTGAAGAAATTCAATGAATTATGCTTGAAGTGCCAAGGAGTTTCTAGTGTTTGCTGGTTACATGGAATTTCAATGTTAAATGGAAGGGTTAGATTTGGACTGTGCATTTATTTCAATGTATAGGATTTTGTTTGCTACCCTTTTGACTTCTATCAAGCTAACACATTTGTTTGTATTCACAGATCTGCATCATTATGAAATTGTATGACGGTTCAATTGGTGACAAGTTGGCTAGACTAAGAGATGGATGGATCTCATTACCTGATGTTTTGAGGTGATGTGTTTTCAGAAAAACATGCTTATCTTTAACTTATATAGCATAGGGAAAATTACTGGTCAATACTAAGGATGTGTTGGTCATTAATGGCAGGTATGGGATCGATCTGGCTCAAGGCATTCTAGAGCTTCACGCTAAAGGGGTCCTTGTTCTCAACCTTAAACCATGTAATGTTCTTCTCAATGACAACGATCAAGCGATTTTGGGAGACGTTGGTATTCCCAATCTTTTGCTTGGCTCTTCATTCGTTAGCTCAGATATTGCTCAGAGGCTTGGAAGTCCTAATTACATGGCTCCAGAACAATGGAAACCAGAGGTCAGAGGCCCTATGTCATTTGAAACGGATTCATGGGGATTCGGATGCACCGTTGTTGAAATGTTGACTGGTAGTCAACCTTGGTATGGATGTCCTGTTGGTGAAATATATGGATCAGTTGTCGAAAAGCATGAAAAGCTGCATATCCCAAGTGGCCTTCCTTCTCCCATTGAGAATATCCTTGGTGCATGTTTTGAGTATGATTTGAGGAATCGACCTTCAATGGTAGATATTCTGCGCGTCTTTAAAAGGTTTGTATGCAGATGTATCAATCTGAAGCCATTTTTTTTTTTCTGATGCCGATTCTTGTTTAATTTAGCATGTAGTTTCAATTTTCAATTATCACTTCACATTGGAGAAAAATACTCACATGAAAACTTCTTAGATTCTTTAAAAGAAATTTCTGTAGTAATGAGTTATGATCATGACCGATTATTGGTGAACCTGTACCTGTCCTGTGAATATTCCAAGATTGGGTAGGTCTGTTGTAGGTCTAGAGCTCAAGGTTAAATGAAAGCCTTCTAGATTAGCTGTATTCCTGTGATGTATTATCTTGGGAAGTGTTTACATTTTGGCTTCCTCTGATCCAGGATAATGTCTGTACCTGAAAGAACTAAATCAATAAGCATGAAGCTGTTTGTGCACGAGCATGGTTATCTTTTTAGTTAATATCTATTTATCTTCATGCAGATAAGAAATAATTTATGTTGAATATTACTTTATTCTTATTCATTCTGCCTATGTTGCAGGTCACTGAATGAACTGGCTAATGATGGAGGAGGATGGAGATATCAAGGAAACATGAAAGTAATTCCAAAATCAGGCAGCACTGACTACACCGAGTGGTTCCTCTCAAAGGATCAACTTCAGGTGGGTGACATGGTCCGATCCAGAAAGCCTCCCAACTCATGCAAAGCTCAAAACATGAATGTCCCAGACGGAACTGTCGTTGGCTTAGAACGTACTGCAGATTATGGATTTGTTCTAGTGAGGGTCCACGGTATTCATGACCCTATAAGAATTCATACATCGACTCTTGAACGCGTCACGAATGGCCTGGCAGCTGGTGATTGGGTACGCTTGAAAGATGAAAAGGAAAAGCACTCACCGGTGGGGATTCTACATTCCATCAATCGCAATGACGGTAGAGCTTCTGTTGGATTTATTGGGCTGCAAACTCTCTGGAATGGAAACCCTTCAGAACTCGAAATGGCAGAATCATTCTGCGTGGGCCAGTTTGTTAGGCCAAAAGAGAATGTGTTAAGCCCTCGATTCGAATGGCGTCGTAAAAGGGGAGGGGCCTCTGCTACTGGTAGGATTTCATGGATACTACCAAATGGGTGTTTGGTTGTCAAGTTTCCAGGGATGGTGAGTTTTGGGAATGAATCAAACACCTTGGCCGATCCTTCCGAGGTTGAAGTTGTTGATTTTAACACTTGTCCTGGGATGGTAGAAAAGTATCAACATGTTGAGGATCATCACTGGGCTGTAAGACCTGTTCTAGTTGTACTAGGCATATTTACAGCTTTGAAACTAGGCATATTAGTTGGAAACAAAGTGAAAAGGTGCAAGAGGTTCAATGCCAAAGAAAGCAAAAACCACTATGTTGAAGGTCAAAACACAAACAGTCCTACAAGGATTATAACCCATGGCAACACAACATGGGGGGTTCCTTCAGTTGCTAATATCCTTTTTAAAGATGGTGCTTAA
Protein sequence

>MS.gene015994.t1

MASSSAASFDYEILDKDTDVPKTAAVPTNRASPWIEPEPLKLQHRIGRGPFGDVWLATLHQSTEDYDEHHEVAAKMLHPIKEDHVKIVLKKFNELCLKCQGVSSVCWLHGISMLNGRICIIMKLYDGSIGDKLARLRDGWISLPDVLRYGIDLAQGILELHAKGVLVLNLKPCNVLLNDNDQAILGDVGIPNLLLGSSFVSSDIAQRLGSPNYMAPEQWKPEVRGPMSFETDSWGFGCTVVEMLTGSQPWYGCPVGEIYGSVVEKHEKLHIPSGLPSPIENILGACFEYDLRNRPSMVDILRVFKRSLNELANDGGGWRYQGNMKVIPKSGSTDYTEWFLSKDQLQVGDMVRSRKPPNSCKAQNMNVPDGTVVGLERTADYGFVLVRVHGIHDPIRIHTSTLERVTNGLAAGDWVRLKDEKEKHSPVGILHSINRNDGRASVGFIGLQTLWNGNPSELEMAESFCVGQFVRPKENVLSPRFEWRRKRGGASATGRISWILPNGCLVVKFPGMVSFGNESNTLADPSEVEVVDFNTCPGMVEKYQHVEDHHWAVRPVLVVLGIFTALKLGILVGNKVKRCKRFNAKESKNHYVEGQNTNSPTRIITHGNTTWGVPSVANILFKDGA