AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016038

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016038.t1 MTR_1g013170 93.186 499 32 2 1 499 1 497 0.0 903
MS.gene016038.t1 MTR_1g013180 65.134 522 104 12 1 498 152 619 0.0 597
MS.gene016038.t1 MTR_7g117705 58.282 489 103 13 1 487 1 390 1.73e-164 473
MS.gene016038.t1 MTR_3g106220 47.297 592 197 17 3 499 6 577 4.61e-152 448
MS.gene016038.t1 MTR_2g450070 44.266 497 222 14 3 454 16 502 1.37e-110 342
MS.gene016038.t1 MTR_2g450070 44.266 497 222 14 3 454 16 502 2.59e-110 342
MS.gene016038.t1 MTR_4g131600 50.147 341 149 7 1 338 16 338 5.31e-95 300
MS.gene016038.t1 MTR_4g121020 57.422 256 107 2 2 257 28 281 8.44e-93 296
MS.gene016038.t1 MTR_4g121020 57.422 256 107 2 2 257 28 281 8.46e-93 296
MS.gene016038.t1 MTR_2g034960 51.536 293 131 3 1 289 25 310 2.73e-92 293
MS.gene016038.t1 MTR_4g131580 47.527 364 171 8 5 361 17 367 9.83e-92 291
MS.gene016038.t1 MTR_4g131570 47.917 336 159 7 3 335 15 337 6.75e-91 289
MS.gene016038.t1 MTR_1g032570 54.135 266 105 3 4 259 10 268 3.20e-90 288
MS.gene016038.t1 MTR_8g079940 56.589 258 102 3 3 256 23 274 1.70e-85 273
MS.gene016038.t1 MTR_1g013160 94.595 148 8 0 67 214 1 148 2.79e-83 254
MS.gene016038.t1 MTR_3g086100 55.823 249 102 2 5 249 13 257 9.01e-83 267
MS.gene016038.t1 MTR_3g102590 51.866 268 72 4 2 266 5 218 1.22e-74 236
MS.gene016038.t1 MTR_3g102590 54.167 240 57 2 2 240 5 192 2.16e-69 220
MS.gene016038.t1 MTR_1g032570 53.398 206 79 3 64 259 1 199 4.03e-63 215
MS.gene016038.t1 MTR_3g102600 41.956 317 84 7 1 317 4 220 5.36e-61 200
MS.gene016038.t1 MTR_3g086100 54.211 190 79 2 64 249 1 186 1.23e-56 196
MS.gene016038.t1 MTR_3g102600 46.888 241 66 4 2 242 5 183 1.82e-54 181
MS.gene016038.t1 MTR_4g021760 39.326 267 132 4 5 271 11 247 2.05e-54 185
MS.gene016038.t1 MTR_4g021790 39.676 247 121 4 2 248 7 225 1.71e-48 168
MS.gene016038.t1 MTR_4g021855 38.462 247 124 3 2 248 7 225 1.72e-48 169
MS.gene016038.t1 MTR_4g021845 38.494 239 119 4 2 240 7 217 8.04e-47 162
MS.gene016038.t1 MTR_4g121020 49.425 174 86 2 84 257 6 177 3.39e-46 169
MS.gene016038.t1 MTR_0450s0040 38.174 241 128 3 9 249 8 227 8.66e-44 158
MS.gene016038.t1 MTR_3g102600 37.269 271 70 7 47 317 1 171 2.25e-41 146
MS.gene016038.t1 MTR_8g105600 36.101 277 150 6 5 274 9 265 3.16e-39 150
MS.gene016038.t1 MTR_7g026400 36.986 219 122 2 32 250 14 216 1.29e-37 146
MS.gene016038.t1 MTR_6g045327 34.286 245 131 3 4 247 13 228 4.63e-37 140
MS.gene016038.t1 MTR_3g102600 67.857 84 27 0 47 130 1 84 1.58e-33 124
MS.gene016038.t1 MTR_3g102600 69.811 53 16 0 190 242 82 134 6.78e-19 83.6
MS.gene016038.t1 MTR_6g453220 79.775 89 18 0 207 295 379 467 4.66e-33 132
MS.gene016038.t1 MTR_5g055260 34.413 247 107 7 2 248 7 198 3.52e-31 121
MS.gene016038.t1 MTR_8g032710 29.688 256 156 5 8 243 20 271 1.47e-27 115
MS.gene016038.t1 MTR_4g064730 47.788 113 53 2 156 262 94 206 1.51e-23 101
MS.gene016038.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 1.13e-22 102
MS.gene016038.t1 MTR_3g092780 34.532 139 86 2 9 143 56 193 1.37e-22 102
MS.gene016038.t1 MTR_6g016850 37.008 127 77 2 3 127 7 132 5.43e-22 93.2
MS.gene016038.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 7.27e-22 99.8
MS.gene016038.t1 MTR_3g092780 37.008 127 75 2 9 131 56 181 9.16e-22 99.4
MS.gene016038.t1 MTR_4g023980 32.934 167 85 4 51 217 35 174 2.60e-21 91.7
MS.gene016038.t1 MTR_7g118260 32.258 186 111 5 2 178 5 184 3.33e-21 97.4
MS.gene016038.t1 MTR_8g024260 33.929 168 97 3 11 164 38 205 1.11e-20 95.9
MS.gene016038.t1 MTR_7g118260 35.766 137 77 3 2 130 5 138 6.04e-20 93.2
MS.gene016038.t1 MTR_7g089010 65.000 60 21 0 190 249 90 149 1.14e-19 89.7
MS.gene016038.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 1.66e-19 92.4
MS.gene016038.t1 MTR_1g067110 36.885 122 74 2 9 127 85 206 1.68e-19 92.4
MS.gene016038.t1 MTR_4g061360 36.220 127 77 2 5 127 84 210 7.27e-19 90.5
MS.gene016038.t1 MTR_3g037390 33.871 124 79 1 9 129 24 147 1.45e-18 89.0
MS.gene016038.t1 MTR_8g093040 35.484 124 80 0 4 127 13 136 2.41e-18 85.5
MS.gene016038.t1 MTR_4g108880 32.258 124 81 1 9 129 33 156 4.03e-17 84.7
MS.gene016038.t1 MTR_2g090675 40.351 114 57 2 188 301 158 260 6.04e-17 83.2
MS.gene016038.t1 MTR_5g014040 54.545 66 30 0 184 249 304 369 7.27e-17 83.6
MS.gene016038.t1 MTR_5g014040 34.677 124 77 2 5 127 14 134 1.34e-11 67.4
MS.gene016038.t1 MTR_4g078810 53.030 66 31 0 188 253 155 220 4.57e-16 80.5
MS.gene016038.t1 MTR_4g098870 55.738 61 27 0 189 249 308 368 2.91e-15 79.0
MS.gene016038.t1 MTR_4g098870 55.738 61 27 0 189 249 308 368 3.10e-15 78.6
MS.gene016038.t1 MTR_4g098870 55.738 61 27 0 189 249 328 388 3.28e-15 78.6
MS.gene016038.t1 MTR_4g098870 55.738 61 27 0 189 249 328 388 3.31e-15 78.6
MS.gene016038.t1 MTR_4g098870 55.738 61 27 0 189 249 347 407 3.45e-15 78.6
MS.gene016038.t1 MTR_5g014040 50.704 71 30 1 184 249 264 334 5.84e-15 77.8
MS.gene016038.t1 MTR_5g014040 50.704 71 30 1 184 249 304 374 7.07e-15 77.4
MS.gene016038.t1 MTR_5g014040 34.677 124 77 2 5 127 14 134 1.62e-11 67.0
MS.gene016038.t1 MTR_4g086835 39.091 110 46 2 186 295 269 357 1.29e-14 76.3
MS.gene016038.t1 MTR_1g093080 50.000 68 33 1 184 250 204 271 5.19e-14 73.9
MS.gene016038.t1 MTR_7g115530 50.000 76 37 1 189 263 216 291 6.90e-14 72.8
MS.gene016038.t1 MTR_7g115530 50.000 76 37 1 189 263 216 291 1.45e-13 72.0
MS.gene016038.t1 MTR_5g017980 51.613 62 30 0 188 249 189 250 1.91e-13 71.6
MS.gene016038.t1 MTR_5g017980 51.613 62 30 0 188 249 190 251 2.04e-13 71.6
MS.gene016038.t1 MTR_4g113140 48.684 76 37 2 176 250 221 295 2.16e-13 72.4
MS.gene016038.t1 MTR_7g115530 50.000 76 37 1 189 263 216 291 2.63e-13 72.4
MS.gene016038.t1 MTR_1g080330 60.345 58 22 1 187 243 171 228 3.27e-13 70.5
MS.gene016038.t1 MTR_7g115530 64.286 56 19 1 189 243 216 271 3.49e-13 71.2
MS.gene016038.t1 MTR_4g086835 38.739 111 46 3 186 295 269 358 3.84e-13 71.6
MS.gene016038.t1 MTR_1g080330 52.941 68 31 1 187 253 171 238 4.57e-13 71.2
MS.gene016038.t1 MTR_2g023580 45.238 84 45 1 190 272 54 137 1.47e-12 67.8
MS.gene016038.t1 MTR_5g017980 50.794 63 30 1 188 249 190 252 1.91e-12 68.6
MS.gene016038.t1 MTR_7g098250 51.471 68 32 1 187 253 258 325 1.95e-12 69.7
MS.gene016038.t1 MTR_6g032990 43.878 98 46 4 191 284 42 134 2.30e-12 68.9
MS.gene016038.t1 MTR_5g054300 49.231 65 32 1 185 248 192 256 3.00e-12 68.6
MS.gene016038.t1 MTR_2g086450 56.364 55 23 1 191 244 45 99 3.59e-12 68.6
MS.gene016038.t1 MTR_6g088950 27.485 171 118 2 7 176 5 170 4.48e-12 65.9
MS.gene016038.t1 MTR_2g027800 57.627 59 24 1 187 244 35 93 5.47e-12 67.0
MS.gene016038.t1 MTR_2g027800 57.627 59 24 1 187 244 35 93 5.51e-12 67.0
MS.gene016038.t1 MTR_1g090670 55.932 59 25 1 187 244 40 98 7.27e-12 66.6
MS.gene016038.t1 MTR_7g115530 62.963 54 19 1 189 241 216 269 9.53e-12 65.9
MS.gene016038.t1 MTR_5g027440 38.235 102 55 3 187 280 21 122 1.15e-11 65.9
MS.gene016038.t1 MTR_7g088070 45.783 83 41 3 191 270 50 131 1.45e-11 65.5
MS.gene016038.t1 MTR_1g053830 54.717 53 23 1 193 244 20 72 1.53e-11 61.2
MS.gene016038.t1 MTR_5g041350 52.727 55 25 1 191 244 15 69 1.94e-11 65.9
MS.gene016038.t1 MTR_8g038620 28.986 138 78 3 11 128 11 148 3.84e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016038.t1 AT2G25180 58.333 336 122 9 3 332 11 334 6.10e-112 344
MS.gene016038.t1 AT4G31920 49.257 404 147 11 3 390 11 372 3.95e-104 322
MS.gene016038.t1 AT3G16857 50.000 306 119 5 3 285 31 325 5.17e-92 294
MS.gene016038.t1 AT3G16857 50.000 306 119 5 3 285 31 325 1.03e-91 294
MS.gene016038.t1 AT5G58080 47.258 383 156 11 3 367 12 366 4.16e-87 280
MS.gene016038.t1 AT2G01760 43.056 360 177 9 1 337 3 357 4.59e-78 249
MS.gene016038.t1 AT1G67710 54.980 251 100 4 6 249 8 252 9.15e-78 253
MS.gene016038.t1 AT3G16857 49.434 265 100 5 44 285 2 255 1.49e-74 247
MS.gene016038.t1 AT2G01760 39.948 388 177 10 1 337 3 385 6.80e-72 234
MS.gene016038.t1 AT1G67710 53.368 193 77 4 64 249 1 187 1.87e-51 181
MS.gene016038.t1 AT5G49240 38.819 237 142 2 5 240 38 272 2.98e-49 171
MS.gene016038.t1 AT2G01760 39.731 297 151 9 64 337 1 292 3.71e-49 171
MS.gene016038.t1 AT3G62670 37.795 254 139 7 10 257 42 282 1.47e-43 159
MS.gene016038.t1 AT1G67710 47.977 173 77 4 84 249 12 178 6.04e-37 142
MS.gene016038.t1 AT1G49190 31.387 274 170 5 4 267 29 294 3.15e-35 136
MS.gene016038.t1 AT5G07210 31.900 279 144 7 7 250 14 281 2.75e-34 136
MS.gene016038.t1 AT5G07210 27.681 401 217 14 7 353 14 395 9.35e-32 129
MS.gene016038.t1 AT2G27070 31.338 284 140 7 7 249 14 283 1.00e-31 129
MS.gene016038.t1 AT2G46790 39.837 123 69 2 9 127 37 158 4.14e-24 105
MS.gene016038.t1 AT2G46790 39.837 123 69 2 9 127 37 158 4.96e-24 105
MS.gene016038.t1 AT5G59570 49.462 93 47 0 170 262 120 212 6.91e-23 99.4
MS.gene016038.t1 AT5G59570 49.462 93 47 0 170 262 120 212 6.91e-23 99.4
MS.gene016038.t1 AT1G49190 37.600 125 78 0 4 128 29 153 1.58e-22 101
MS.gene016038.t1 AT5G24470 36.719 128 76 2 7 130 48 174 1.03e-21 99.0
MS.gene016038.t1 AT3G46640 37.107 159 86 4 104 249 45 202 3.75e-21 94.7
MS.gene016038.t1 AT3G46640 37.107 159 86 4 104 249 45 202 3.75e-21 94.7
MS.gene016038.t1 AT2G40970 45.370 108 55 1 169 272 79 186 4.08e-21 93.2
MS.gene016038.t1 AT3G46640 37.107 159 86 4 104 249 45 202 6.59e-21 94.0
MS.gene016038.t1 AT5G02810 38.525 122 72 1 9 127 78 199 1.36e-20 95.9
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.71e-20 93.6
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.71e-20 93.6
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 2.71e-20 93.6
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 4.88e-20 93.6
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 4.88e-20 93.6
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 4.88e-20 93.6
MS.gene016038.t1 AT5G05090 51.899 79 38 0 171 249 61 139 5.00e-20 90.1
MS.gene016038.t1 AT3G10760 65.000 60 21 0 190 249 104 163 5.05e-20 91.7
MS.gene016038.t1 AT5G60100 37.795 127 75 2 5 127 59 185 1.02e-19 92.4
MS.gene016038.t1 AT5G44190 59.155 71 29 0 183 253 139 209 1.28e-19 90.9
MS.gene016038.t1 AT5G44190 59.155 71 29 0 183 253 139 209 1.40e-19 90.9
MS.gene016038.t1 AT5G61380 33.871 124 79 1 9 129 19 142 6.36e-19 90.5
MS.gene016038.t1 AT2G20570 39.370 127 64 2 140 253 89 215 2.45e-17 84.7
MS.gene016038.t1 AT2G20570 63.793 58 21 0 196 253 174 231 8.28e-16 80.1
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 9.91e-15 77.0
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 9.91e-15 77.0
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 9.91e-15 77.0
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 9.91e-15 77.0
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 9.91e-15 77.0
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 9.91e-15 77.0
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 1.25e-14 76.6
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 1.25e-14 76.6
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 1.25e-14 76.6
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 1.25e-14 76.6
MS.gene016038.t1 AT4G18020 54.286 70 30 1 185 254 292 359 1.25e-14 76.6
MS.gene016038.t1 AT2G03500 54.839 62 27 1 190 250 232 293 4.05e-13 71.6
MS.gene016038.t1 AT5G45580 53.731 67 28 2 181 244 11 77 1.79e-12 68.2
MS.gene016038.t1 AT3G57040 28.395 162 94 2 11 150 11 172 2.30e-12 67.4
MS.gene016038.t1 AT3G57040 28.395 162 94 2 11 150 11 172 2.30e-12 67.4
MS.gene016038.t1 AT3G04030 56.364 55 23 1 191 244 45 99 2.83e-12 68.9
MS.gene016038.t1 AT5G45580 55.932 59 25 1 187 244 3 61 3.00e-12 67.4
MS.gene016038.t1 AT3G04030 56.364 55 23 1 191 244 45 99 3.08e-12 68.6
MS.gene016038.t1 AT5G18240 58.182 55 22 1 191 244 45 99 5.65e-12 67.8
MS.gene016038.t1 AT5G18240 58.182 55 22 1 191 244 45 99 5.65e-12 67.8
MS.gene016038.t1 AT5G18240 58.182 55 22 1 191 244 45 99 6.01e-12 67.8
MS.gene016038.t1 AT5G18240 58.182 55 22 1 191 244 45 99 6.51e-12 67.8
MS.gene016038.t1 AT5G18240 58.182 55 22 1 191 244 45 99 6.51e-12 67.8
MS.gene016038.t1 AT3G56380 31.298 131 76 2 9 125 20 150 9.75e-12 63.5
MS.gene016038.t1 AT1G25550 51.667 60 28 1 190 248 208 267 2.28e-11 65.9
MS.gene016038.t1 AT2G40670 32.331 133 74 2 9 125 29 161 2.41e-11 62.8
MS.gene016038.t1 AT3G56380 31.538 130 76 2 9 125 20 149 3.43e-11 62.0
MS.gene016038.t1 AT1G49560 47.222 72 37 1 186 256 187 258 4.96e-11 64.7
MS.gene016038.t1 AT1G69580 54.545 55 24 1 191 244 34 88 5.90e-11 64.3
MS.gene016038.t1 AT3G24120 41.111 90 42 2 187 265 37 126 6.15e-11 63.9
MS.gene016038.t1 AT3G24120 41.111 90 42 2 187 265 37 126 6.16e-11 63.9
MS.gene016038.t1 AT1G69580 54.545 55 24 1 191 244 34 88 6.27e-11 64.3
MS.gene016038.t1 AT4G37180 49.180 61 30 1 189 248 208 268 6.41e-11 64.3
MS.gene016038.t1 AT1G68670 42.857 77 43 1 173 248 197 273 6.57e-11 64.3
MS.gene016038.t1 AT4G37180 49.180 61 30 1 189 248 215 275 6.96e-11 64.3
MS.gene016038.t1 AT2G40670 32.576 132 74 2 9 125 29 160 7.46e-11 61.2

Find 107 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1.4:-8030672 MS.gene016038:CDS
TTGTTTGTGAAACTGAAATT+TGG 0.235409 1.4:+8028504 None:intergenic
GGAGGTTATTGGTCATGTTT+TGG 0.244923 1.4:+8028526 None:intergenic
ACTGAAATTTGGGAGGTTAT+TGG 0.250594 1.4:+8028515 None:intergenic
ATTCCTTTCATCACCATCTT+TGG 0.256348 1.4:+8029830 None:intergenic
ATCTAAAAGGATTGTTATAA+TGG 0.273251 1.4:+8028751 None:intergenic
CATTTGGATTGCCGCGAGTT+AGG 0.293700 1.4:+8028580 None:intergenic
ACAGAAGGTGCAATGGTATT+TGG 0.304024 1.4:-8028383 MS.gene016038:CDS
TGTACCTCTTTCAGTCTAAC+AGG 0.317233 1.4:+8029776 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1.4:+8029692 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1.4:-8030722 MS.gene016038:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1.4:-8029692 MS.gene016038:CDS
ATACCTCTTTGAACATCATT+TGG 0.333499 1.4:+8028564 None:intergenic
CCAGATGGAAAGGATCTAAA+AGG 0.348535 1.4:+8028738 None:intergenic
TCCAACTCCACTTCTTAAAT+AGG 0.361147 1.4:+8028815 None:intergenic
GGAAGATAAGAGTTTGTTCC+AGG 0.362766 1.4:+8028339 None:intergenic
ATAGGCTCTTCTATGATTCC+TGG 0.370372 1.4:-8028207 MS.gene016038:CDS
GGAAACTTGGACTACAATTA+TGG 0.374368 1.4:-8028162 MS.gene016038:CDS
TGTTTGTGAAACTGAAATTT+GGG 0.375065 1.4:+8028505 None:intergenic
AACAATCCCTTGATGTTAGA+AGG 0.383955 1.4:-8028486 MS.gene016038:CDS
TTTAAGCTCCTTGAGCTTGT+TGG 0.387237 1.4:-8029985 MS.gene016038:CDS
ACATGGTGCGTGTGATTATC+TGG 0.387305 1.4:-8029804 MS.gene016038:CDS
ATTTGGATTGCCGCGAGTTA+GGG 0.388130 1.4:+8028581 None:intergenic
TGAAAGAGGTACAGATCATT+TGG 0.404224 1.4:-8029766 MS.gene016038:CDS
ATGCCTCATGTGTCATTTCA+AGG 0.407109 1.4:-8028291 MS.gene016038:CDS
AATGTGATCGGCAGTTCAAT+AGG 0.412930 1.4:-8028225 MS.gene016038:CDS
TATGAACATTGCTGATTACT+AGG 0.423694 1.4:+8030025 None:intergenic
TCATGTGTCATTTCAAGGTT+GGG 0.431069 1.4:-8028286 MS.gene016038:CDS
TACCATGGTATTGACACCTT+TGG 0.431203 1.4:+8030639 None:intergenic
CCATTCATATTCACATTAGT+AGG 0.431732 1.4:+8028687 None:intergenic
GGACATTTGCATACATTGAA+TGG 0.439183 1.4:-8028792 MS.gene016038:CDS
GTTGCTGCGGTTAATCAATT+AGG 0.445001 1.4:-8029494 MS.gene016038:CDS
CCTACTAATGTGAATATGAA+TGG 0.451724 1.4:-8028687 MS.gene016038:CDS
TCATTTGGCAGCATGTAATC+AGG 0.452747 1.4:-8029751 MS.gene016038:CDS
GACGCGCCTAATTCTGATAG+TGG 0.455329 1.4:-8029698 MS.gene016038:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1.4:-8029541 MS.gene016038:CDS
CTGACTCGGCTGAAGAGTTC+CGG 0.455864 1.4:+8028653 None:intergenic
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1.4:-8029974 MS.gene016038:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1.4:+8030707 None:intergenic
AGAATTTCCTTCTAACATCA+AGG 0.459500 1.4:+8028479 None:intergenic
GGACTACAATTATGGTGATT+CGG 0.460683 1.4:-8028154 MS.gene016038:CDS
ATCAGTTCAAGAATCTTCTT+CGG 0.461313 1.4:+8029213 None:intergenic
CTCATGTGTCATTTCAAGGT+TGG 0.464023 1.4:-8028287 MS.gene016038:CDS
AGCTTAAATCCATCCATGTC+TGG 0.471845 1.4:+8030000 None:intergenic
GCATGGTTAAAAGATTCTCT+TGG 0.472134 1.4:+8028318 None:intergenic
GGGAAACATTCGGCTGGGAC+AGG 0.477461 1.4:-8029671 MS.gene016038:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1.4:-8029691 MS.gene016038:CDS
ATGAAAAGCCATCTAGAAAA+CGG 0.489677 1.4:-8029634 MS.gene016038:CDS
GATACATCCTATTTAAGAAG+TGG 0.493596 1.4:-8028822 MS.gene016038:CDS
TCCTATTTAAGAAGTGGAGT+TGG 0.497128 1.4:-8028816 MS.gene016038:CDS
GATAATAACAATAATCATGA+TGG 0.497207 1.4:-8028264 MS.gene016038:CDS
CACTGCTCAAAACGCGATAA+CGG 0.499559 1.4:-8030085 MS.gene016038:CDS
TTTAGATCCTTTCCATCTGG+TGG 0.500957 1.4:-8028735 MS.gene016038:CDS
TCCCTTTACTTGAAAACAGA+AGG 0.502275 1.4:-8028398 MS.gene016038:CDS
GGAAGACAAACAAGATCATA+TGG 0.510045 1.4:-8028868 MS.gene016038:CDS
TAGTGGAAATGGGAAACATT+CGG 0.510168 1.4:-8029681 MS.gene016038:CDS
AGCACAATGCAACAACTACC+TGG 0.515145 1.4:-8028357 MS.gene016038:CDS
GGAAATGGGAAACATTCGGC+TGG 0.517134 1.4:-8029677 MS.gene016038:CDS
TGCCGATCACATTAGAAGAA+TGG 0.518061 1.4:+8028235 None:intergenic
TACCATTCTTCTAATGTGAT+CGG 0.518616 1.4:-8028237 MS.gene016038:CDS
GAAGGTGGCCAGCCATCTCC+AGG 0.523004 1.4:-8029166 MS.gene016038:intron
TTGCATAACTTACTTATGAC+AGG 0.526242 1.4:+8029946 None:intergenic
TATTTAAGAAGTGGAGTTGG+AGG 0.527033 1.4:-8028813 MS.gene016038:CDS
TGCCGCGAGTTAGGGCCGAT+TGG 0.528037 1.4:+8028589 None:intergenic
ATGAATGGATTGCATTCTGC+CGG 0.538303 1.4:-8028672 MS.gene016038:CDS
GAAACCTGTTAGACTGAAAG+AGG 0.539886 1.4:-8029780 MS.gene016038:CDS
TGTCGAGCAAGAAGGAAACT+TGG 0.540152 1.4:-8028175 MS.gene016038:CDS
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1.4:-8030705 MS.gene016038:CDS
TCAGCGGACCAAACAGTTCG+AGG 0.550196 1.4:+8029533 None:intergenic
AAGGATAAGAATGAAGATGA+TGG 0.554575 1.4:-8029611 MS.gene016038:CDS
CAATGTTCATATGCCAGACA+TGG 0.556391 1.4:-8030013 MS.gene016038:CDS
CTTGAAAACAGAAGGTGCAA+TGG 0.560167 1.4:-8028390 MS.gene016038:CDS
GAATTTCCTTCTAACATCAA+GGG 0.562420 1.4:+8028480 None:intergenic
ATGGTGATTCGGTACAATGA+AGG 0.563231 1.4:-8028143 None:intergenic
TAAGAATGAAGATGATGGTG+AGG 0.566326 1.4:-8029606 MS.gene016038:CDS
TGATCAGCTAAAGTTCCAAT+CGG 0.568120 1.4:-8028604 MS.gene016038:CDS
TGTATCTGAACCACTAGCTA+TGG 0.569493 1.4:+8028839 None:intergenic
ATGAATGTTGTCGAGCAAGA+AGG 0.574186 1.4:-8028183 MS.gene016038:CDS
TGATGGTGAGGAAGAAAGTG+AGG 0.580573 1.4:-8029594 MS.gene016038:CDS
CATAACACCACCAGATGGAA+AGG 0.581151 1.4:+8028728 None:intergenic
GTTCATATGCCAGACATGGA+TGG 0.584384 1.4:-8030009 MS.gene016038:CDS
TGCTCGACAACATTCATACC+AGG 0.593541 1.4:+8028189 None:intergenic
TTTAAATTATGTGTCTGACT+CGG 0.594323 1.4:+8028639 None:intergenic
TCTCAAGTCCAACAAGCTCA+AGG 0.597253 1.4:+8029977 None:intergenic
AATTCTCAAGACAAACAAGT+AGG 0.598098 1.4:-8028462 MS.gene016038:CDS
CAAGACAAACAAGTAGGTGA+AGG 0.598346 1.4:-8028456 MS.gene016038:CDS
TGAAAATCTGACAAGGGAGA+AGG 0.601495 1.4:-8029184 MS.gene016038:CDS
AATCCAAATGATGTTCAAAG+AGG 0.602398 1.4:-8028567 MS.gene016038:CDS
TTCCAATCGGCCCTAACTCG+CGG 0.602702 1.4:-8028591 MS.gene016038:CDS
AAATCTGACAAGGGAGAAGG+TGG 0.609003 1.4:-8029181 MS.gene016038:CDS
ACACCGCAAGTTTGTTGCTG+CGG 0.610830 1.4:-8029507 MS.gene016038:CDS
TGCGAATGATGATCCAAAGA+TGG 0.615837 1.4:-8029843 MS.gene016038:CDS
CTACTCATAACACCACCAGA+TGG 0.616853 1.4:+8028723 None:intergenic
GAAATGGGAAACATTCGGCT+GGG 0.620073 1.4:-8029676 MS.gene016038:CDS
AAAGCCATCTAGAAAACGGA+AGG 0.622229 1.4:-8029630 MS.gene016038:CDS
ATAACTTACTTATGACAGGT+AGG 0.629417 1.4:+8029950 None:intergenic
GATCCAAAGATGGTGATGAA+AGG 0.637771 1.4:-8029833 MS.gene016038:CDS
ACACATGAGGCATAGCAGCA+TGG 0.642777 1.4:+8028301 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1.4:-8030641 MS.gene016038:intron
ATGGCAGCAGCCATAGCTAG+TGG 0.659987 1.4:-8028849 MS.gene016038:CDS
TAACCGCAGCAACAAACTTG+CGG 0.661176 1.4:+8029504 None:intergenic
AAACTTGCGGTGTAAATCAG+CGG 0.665866 1.4:+8029517 None:intergenic
CAACCTTGAAATGACACATG+AGG 0.667024 1.4:+8028288 None:intergenic
TTGTGAAACTGAAATTTGGG+AGG 0.670570 1.4:+8028508 None:intergenic
TGAATGTTGAAAATCTGACA+AGG 0.702505 1.4:-8029191 MS.gene016038:CDS
GAATGTTGAAAATCTGACAA+GGG 0.715396 1.4:-8029190 MS.gene016038:CDS
GTGATGAAAGGAATTGAACA+TGG 0.742148 1.4:-8029821 MS.gene016038:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGATATTTATTTTAGTTA+TGG - chr1.4:8028738-8028757 MS.gene016038:CDS 10.0%
!!! AATTTTATTTGATATTGTTT+AGG - chr1.4:8028516-8028535 MS.gene016038:CDS 10.0%
!!! ATTATTATTTTCAATAAAGT+TGG - chr1.4:8028414-8028433 MS.gene016038:CDS 10.0%
!!! TATTTTGAAATATTAAAGAT+GGG - chr1.4:8028291-8028310 MS.gene016038:CDS 10.0%
!!! TTATTTTGAAATATTAAAGA+TGG - chr1.4:8028290-8028309 MS.gene016038:CDS 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + chr1.4:8028318-8028337 None:intergenic 15.0%
!! TAATTGAATTTAACAACATA+TGG - chr1.4:8029840-8029859 MS.gene016038:CDS 15.0%
!! TATCAATAATATAAGATTGA+AGG + chr1.4:8028950-8028969 None:intergenic 15.0%
!!! TTTTTAGTAAAGAATATTTG+AGG + chr1.4:8029554-8029573 None:intergenic 15.0%
!! AAATCTAATGATATATAGCT+AGG - chr1.4:8028346-8028365 MS.gene016038:CDS 20.0%
!! AATCTAATGATATATAGCTA+GGG - chr1.4:8028347-8028366 MS.gene016038:CDS 20.0%
!! ATCTAAAAGGATTGTTATAA+TGG + chr1.4:8030120-8030139 None:intergenic 20.0%
!! GATAATAACAATAATCATGA+TGG - chr1.4:8030604-8030623 MS.gene016038:intron 20.0%
!!! ATTTTGATAAACTGGAAAAA+AGG + chr1.4:8029488-8029507 None:intergenic 20.0%
! CTGCAGAAAAATTAAAAAAC+AGG + chr1.4:8029945-8029964 None:intergenic 25.0%
! GAAGTCTTCAAATAATACAT+TGG + chr1.4:8029532-8029551 None:intergenic 25.0%
! TCTAAAAAGAATTAGTTGTG+CGG - chr1.4:8029979-8029998 MS.gene016038:CDS 25.0%
! TCTAGCATATGCATAAATAT+AGG + chr1.4:8028395-8028414 None:intergenic 25.0%
! TGTTTGTGAAACTGAAATTT+GGG + chr1.4:8030366-8030385 None:intergenic 25.0%
! TTGTTTGTGAAACTGAAATT+TGG + chr1.4:8030367-8030386 None:intergenic 25.0%
! TTTAAATTATGTGTCTGACT+CGG + chr1.4:8030232-8030251 None:intergenic 25.0%
!! TAATTGATTTCTACAAGCAT+TGG + chr1.4:8028485-8028504 None:intergenic 25.0%
!!! GCACAAATATTTTGATAAAC+TGG + chr1.4:8029496-8029515 None:intergenic 25.0%
AAGGATAAGAATGAAGATGA+TGG - chr1.4:8029257-8029276 MS.gene016038:intron 30.0%
AATCCAAATGATGTTCAAAG+AGG - chr1.4:8030301-8030320 MS.gene016038:intron 30.0%
AATTCTCAAGACAAACAAGT+AGG - chr1.4:8030406-8030425 MS.gene016038:intron 30.0%
ACATAAGATCACCAAAGATT+AGG + chr1.4:8028644-8028663 None:intergenic 30.0%
AGAATTTCCTTCTAACATCA+AGG + chr1.4:8030392-8030411 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + chr1.4:8028921-8028940 None:intergenic 30.0%
ATACCTCTTTGAACATCATT+TGG + chr1.4:8030307-8030326 None:intergenic 30.0%
ATCAGTTCAAGAATCTTCTT+CGG + chr1.4:8029658-8029677 None:intergenic 30.0%
ATGAAAAGCCATCTAGAAAA+CGG - chr1.4:8029234-8029253 MS.gene016038:intron 30.0%
ATGAATGATCAGTTTCCTAT+TGG - chr1.4:8028146-8028165 MS.gene016038:CDS 30.0%
CCATTCATATTCACATTAGT+AGG + chr1.4:8030184-8030203 None:intergenic 30.0%
CCTACTAATGTGAATATGAA+TGG - chr1.4:8030181-8030200 MS.gene016038:intron 30.0%
GAATGTTGAAAATCTGACAA+GGG - chr1.4:8029678-8029697 MS.gene016038:CDS 30.0%
GAATTTCCTTCTAACATCAA+GGG + chr1.4:8030391-8030410 None:intergenic 30.0%
GATACATCCTATTTAAGAAG+TGG - chr1.4:8030046-8030065 MS.gene016038:CDS 30.0%
TACCATTCTTCTAATGTGAT+CGG - chr1.4:8030631-8030650 MS.gene016038:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - chr1.4:8029677-8029696 MS.gene016038:CDS 30.0%
TGAGAAAAACACTTCAGAAT+CGG + chr1.4:8029607-8029626 None:intergenic 30.0%
TTGCATAACTTACTTATGAC+AGG + chr1.4:8028925-8028944 None:intergenic 30.0%
! ATGATATATAGCTAGGGTTT+TGG - chr1.4:8028353-8028372 MS.gene016038:CDS 30.0%
! TAGAACATGTCTCAAGATTT+TGG - chr1.4:8028196-8028215 MS.gene016038:CDS 30.0%
! TATGAACATTGCTGATTACT+AGG + chr1.4:8028846-8028865 None:intergenic 30.0%
! TTGTTGCTTCTTTCAAAGAT+TGG - chr1.4:8028569-8028588 MS.gene016038:CDS 30.0%
!! AAGAGATAAGAAAAAGTACC+TGG + chr1.4:8029723-8029742 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - chr1.4:8029177-8029196 MS.gene016038:CDS 30.0%
!! TTATTGGTCATGTTTTGGAT+AGG + chr1.4:8030340-8030359 None:intergenic 30.0%
!!! ACCTTCTGTTTTCAAGTAAA+GGG + chr1.4:8030474-8030493 None:intergenic 30.0%
AACAATCCCTTGATGTTAGA+AGG - chr1.4:8030382-8030401 MS.gene016038:intron 35.0%
AATCGGAAAAATTCGTAGCA+TGG + chr1.4:8029590-8029609 None:intergenic 35.0%
ACTGAAATTTGGGAGGTTAT+TGG + chr1.4:8030356-8030375 None:intergenic 35.0%
ATCTTTGTGTGCCTAATCTT+TGG - chr1.4:8028630-8028649 MS.gene016038:CDS 35.0%
ATTCCTTTCATCACCATCTT+TGG + chr1.4:8029041-8029060 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + chr1.4:8029179-8029198 None:intergenic 35.0%
GCATGGTTAAAAGATTCTCT+TGG + chr1.4:8030553-8030572 None:intergenic 35.0%
GGAAACTTGGACTACAATTA+TGG - chr1.4:8030706-8030725 MS.gene016038:CDS 35.0%
GGAAGACAAACAAGATCATA+TGG - chr1.4:8030000-8030019 MS.gene016038:CDS 35.0%
GGACATTTGCATACATTGAA+TGG - chr1.4:8030076-8030095 MS.gene016038:CDS 35.0%
GGACTACAATTATGGTGATT+CGG - chr1.4:8030714-8030733 MS.gene016038:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - chr1.4:8029047-8029066 MS.gene016038:intron 35.0%
TAGTGGAAATGGGAAACATT+CGG - chr1.4:8029187-8029206 MS.gene016038:CDS 35.0%
TATTTAAGAAGTGGAGTTGG+AGG - chr1.4:8030055-8030074 MS.gene016038:CDS 35.0%
TCCAACTCCACTTCTTAAAT+AGG + chr1.4:8030056-8030075 None:intergenic 35.0%
TCCCTTTACTTGAAAACAGA+AGG - chr1.4:8030470-8030489 MS.gene016038:intron 35.0%
TCCTATTTAAGAAGTGGAGT+TGG - chr1.4:8030052-8030071 MS.gene016038:CDS 35.0%
TGATAGTTGTTATCTTGCAG+AGG - chr1.4:8029627-8029646 MS.gene016038:CDS 35.0%
TGATCAGCTAAAGTTCCAAT+CGG - chr1.4:8030264-8030283 MS.gene016038:intron 35.0%
TTGTGAAACTGAAATTTGGG+AGG + chr1.4:8030363-8030382 None:intergenic 35.0%
! TAAGAATGAAGATGATGGTG+AGG - chr1.4:8029262-8029281 MS.gene016038:intron 35.0%
! TGAAAGAGGTACAGATCATT+TGG - chr1.4:8029102-8029121 MS.gene016038:intron 35.0%
! TTATCCTTCCGTTTTCTAGA+TGG + chr1.4:8029245-8029264 None:intergenic 35.0%
! TTTTGCGCATTGAAGAAAGA+GGG + chr1.4:8029417-8029436 None:intergenic 35.0%
! TTTTTGCGCATTGAAGAAAG+AGG + chr1.4:8029418-8029437 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - chr1.4:8029176-8029195 MS.gene016038:CDS 35.0%
!! GGTGATCTTATGTTGTTTCT+TGG - chr1.4:8028651-8028670 MS.gene016038:CDS 35.0%
!! TAAGAAAAAGTACCTGGAGA+TGG + chr1.4:8029717-8029736 None:intergenic 35.0%
!! TCATGTGTCATTTCAAGGTT+GGG - chr1.4:8030582-8030601 MS.gene016038:intron 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - chr1.4:8028213-8028232 MS.gene016038:CDS 35.0%
!!! CACCTTCTGTTTTCAAGTAA+AGG + chr1.4:8030475-8030494 None:intergenic 35.0%
AAACTTGCGGTGTAAATCAG+CGG + chr1.4:8029354-8029373 None:intergenic 40.0%
AAAGCCATCTAGAAAACGGA+AGG - chr1.4:8029238-8029257 MS.gene016038:intron 40.0%
AATGTGATCGGCAGTTCAAT+AGG - chr1.4:8030643-8030662 MS.gene016038:CDS 40.0%
AGCTTAAATCCATCCATGTC+TGG + chr1.4:8028871-8028890 None:intergenic 40.0%
ATAGGCTCTTCTATGATTCC+TGG - chr1.4:8030661-8030680 MS.gene016038:CDS 40.0%
ATGAATGTTGTCGAGCAAGA+AGG - chr1.4:8030685-8030704 MS.gene016038:CDS 40.0%
ATGGTGATTCGTGTTAGTGT+CGG - chr1.4:8029859-8029878 MS.gene016038:intron 40.0%
ATTAGCAGTGTCCATGTGTA+AGG + chr1.4:8029803-8029822 None:intergenic 40.0%
CAACCTTGAAATGACACATG+AGG + chr1.4:8030583-8030602 None:intergenic 40.0%
CAAGACAAACAAGTAGGTGA+AGG - chr1.4:8030412-8030431 MS.gene016038:intron 40.0%
CAATGTTCATATGCCAGACA+TGG - chr1.4:8028855-8028874 MS.gene016038:CDS 40.0%
CCAGATGGAAAGGATCTAAA+AGG + chr1.4:8030133-8030152 None:intergenic 40.0%
CGATGCTTCGTAACTTCTAA+AGG + chr1.4:8029754-8029773 None:intergenic 40.0%
GAAACCTGTTAGACTGAAAG+AGG - chr1.4:8029088-8029107 MS.gene016038:intron 40.0%
GTTGCTGCGGTTAATCAATT+AGG - chr1.4:8029374-8029393 MS.gene016038:intron 40.0%
TATTGGAATGCGAGTTCTTG+CGG - chr1.4:8028163-8028182 MS.gene016038:CDS 40.0%
TCATTTGGCAGCATGTAATC+AGG - chr1.4:8029117-8029136 MS.gene016038:intron 40.0%
TGAAAATCTGACAAGGGAGA+AGG - chr1.4:8029684-8029703 MS.gene016038:CDS 40.0%
TGCCGATCACATTAGAAGAA+TGG + chr1.4:8030636-8030655 None:intergenic 40.0%
TGCGAATGATGATCCAAAGA+TGG - chr1.4:8029025-8029044 MS.gene016038:intron 40.0%
TGTACCTCTTTCAGTCTAAC+AGG + chr1.4:8029095-8029114 None:intergenic 40.0%
TGTATCTGAACCACTAGCTA+TGG + chr1.4:8030032-8030051 None:intergenic 40.0%
TTACACATGGACACTGCTAA+TGG - chr1.4:8029802-8029821 MS.gene016038:CDS 40.0%
TTTAAGCTCCTTGAGCTTGT+TGG - chr1.4:8028883-8028902 MS.gene016038:CDS 40.0%
TTTAGATCCTTTCCATCTGG+TGG - chr1.4:8030133-8030152 MS.gene016038:intron 40.0%
! ACAGAAGGTGCAATGGTATT+TGG - chr1.4:8030485-8030504 MS.gene016038:intron 40.0%
! CCTTTTAGATCCTTTCCATC+TGG - chr1.4:8030130-8030149 MS.gene016038:intron 40.0%
! CTTGAAAACAGAAGGTGCAA+TGG - chr1.4:8030478-8030497 MS.gene016038:intron 40.0%
! GATCCAAAGATGGTGATGAA+AGG - chr1.4:8029035-8029054 MS.gene016038:intron 40.0%
! GGAAGATAAGAGTTTGTTCC+AGG + chr1.4:8030532-8030551 None:intergenic 40.0%
! GGAGGTTATTGGTCATGTTT+TGG + chr1.4:8030345-8030364 None:intergenic 40.0%
!! ATGAATGGATTGCATTCTGC+CGG - chr1.4:8030196-8030215 MS.gene016038:intron 40.0%
!! ATGCCTCATGTGTCATTTCA+AGG - chr1.4:8030577-8030596 MS.gene016038:intron 40.0%
!! CTCATGTGTCATTTCAAGGT+TGG - chr1.4:8030581-8030600 MS.gene016038:intron 40.0%
!! GTTGATGATGAAGCATACCA+TGG + chr1.4:8028247-8028266 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + chr1.4:8028232-8028251 None:intergenic 40.0%
!! TGATTTCTACAAGCATTGGC+TGG + chr1.4:8028481-8028500 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTATGTTT+TGG - chr1.4:8028364-8028383 MS.gene016038:CDS 40.0%
AAATCTGACAAGGGAGAAGG+TGG - chr1.4:8029687-8029706 MS.gene016038:CDS 45.0%
ACATGGTGCGTGTGATTATC+TGG - chr1.4:8029064-8029083 MS.gene016038:intron 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - chr1.4:8029327-8029346 MS.gene016038:intron 45.0%
AGCACAATGCAACAACTACC+TGG - chr1.4:8030511-8030530 MS.gene016038:intron 45.0%
ATTTGGATTGCCGCGAGTTA+GGG + chr1.4:8030290-8030309 None:intergenic 45.0%
CACTGCTCAAAACGCGATAA+CGG - chr1.4:8028783-8028802 MS.gene016038:CDS 45.0%
CATAACACCACCAGATGGAA+AGG + chr1.4:8030143-8030162 None:intergenic 45.0%
CTACTCATAACACCACCAGA+TGG + chr1.4:8030148-8030167 None:intergenic 45.0%
GAAATGGGAAACATTCGGCT+GGG - chr1.4:8029192-8029211 MS.gene016038:CDS 45.0%
GCAAGAACTCGCATTCCAAT+AGG + chr1.4:8028164-8028183 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - chr1.4:8028859-8028878 MS.gene016038:CDS 45.0%
TAACCGCAGCAACAAACTTG+CGG + chr1.4:8029367-8029386 None:intergenic 45.0%
TCTCAAGTCCAACAAGCTCA+AGG + chr1.4:8028894-8028913 None:intergenic 45.0%
TGCTCGACAACATTCATACC+AGG + chr1.4:8030682-8030701 None:intergenic 45.0%
TGTCGAGCAAGAAGGAAACT+TGG - chr1.4:8030693-8030712 MS.gene016038:CDS 45.0%
! TGATGGTGAGGAAGAAAGTG+AGG - chr1.4:8029274-8029293 MS.gene016038:intron 45.0%
!! AAAAAGTACCTGGAGATGGC+TGG + chr1.4:8029713-8029732 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - chr1.4:8028227-8028246 MS.gene016038:CDS 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - chr1.4:8028894-8028913 MS.gene016038:CDS 45.0%
!! TTTCTACAAGCATTGGCTGG+AGG + chr1.4:8028478-8028497 None:intergenic 45.0%
ACACATGAGGCATAGCAGCA+TGG + chr1.4:8030570-8030589 None:intergenic 50.0%
CATTTGGATTGCCGCGAGTT+AGG + chr1.4:8030291-8030310 None:intergenic 50.0%
CCATGTGTAAGGCTCGTTAC+AGG + chr1.4:8029792-8029811 None:intergenic 50.0%
CCTGTAACGAGCCTTACACA+TGG - chr1.4:8029789-8029808 MS.gene016038:CDS 50.0%
GACGCGCCTAATTCTGATAG+TGG - chr1.4:8029170-8029189 MS.gene016038:CDS 50.0%
GGAAATGGGAAACATTCGGC+TGG - chr1.4:8029191-8029210 MS.gene016038:CDS 50.0%
GGATGCAGAATGCTCAGTGT+CGG - chr1.4:8029880-8029899 MS.gene016038:intron 50.0%
!! ACACCGCAAGTTTGTTGCTG+CGG - chr1.4:8029361-8029380 MS.gene016038:intron 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + chr1.4:8028785-8028804 None:intergenic 50.0%
ATGGCAGCAGCCATAGCTAG+TGG - chr1.4:8030019-8030038 MS.gene016038:CDS 55.0%
TCAGCGGACCAAACAGTTCG+AGG + chr1.4:8029338-8029357 None:intergenic 55.0%
TTCCAATCGGCCCTAACTCG+CGG - chr1.4:8030277-8030296 MS.gene016038:intron 55.0%
!! CTGACTCGGCTGAAGAGTTC+CGG + chr1.4:8030218-8030237 None:intergenic 55.0%
GGGAAACATTCGGCTGGGAC+AGG - chr1.4:8029197-8029216 MS.gene016038:CDS 60.0%
GAAGGTGGCCAGCCATCTCC+AGG - chr1.4:8029702-8029721 MS.gene016038:CDS 65.0%
TGCCGCGAGTTAGGGCCGAT+TGG + chr1.4:8030282-8030301 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 8028146 8030744 8028146 ID=MS.gene016038
chr1.4 mRNA 8028146 8030744 8028146 ID=MS.gene016038.t1;Parent=MS.gene016038
chr1.4 exon 8030642 8030744 8030642 ID=MS.gene016038.t1.exon1;Parent=MS.gene016038.t1
chr1.4 CDS 8030642 8030744 8030642 ID=cds.MS.gene016038.t1;Parent=MS.gene016038.t1
chr1.4 exon 8029959 8030111 8029959 ID=MS.gene016038.t1.exon2;Parent=MS.gene016038.t1
chr1.4 CDS 8029959 8030111 8029959 ID=cds.MS.gene016038.t1;Parent=MS.gene016038.t1
chr1.4 exon 8029486 8029872 8029486 ID=MS.gene016038.t1.exon3;Parent=MS.gene016038.t1
chr1.4 CDS 8029486 8029872 8029486 ID=cds.MS.gene016038.t1;Parent=MS.gene016038.t1
chr1.4 exon 8029167 8029243 8029167 ID=MS.gene016038.t1.exon4;Parent=MS.gene016038.t1
chr1.4 CDS 8029167 8029243 8029167 ID=cds.MS.gene016038.t1;Parent=MS.gene016038.t1
chr1.4 exon 8028146 8028925 8028146 ID=MS.gene016038.t1.exon5;Parent=MS.gene016038.t1
chr1.4 CDS 8028146 8028925 8028146 ID=cds.MS.gene016038.t1;Parent=MS.gene016038.t1
Gene Sequence

>MS.gene016038

ATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGGTATGCTTCATCATCAACATCATATTATTGTTGTTTTTGTTTTATTTTGAAATATTAAAGATGGGTCCTTGATTTTAATCTTGTTTTTTGATGTGACAAATCTAATGATATATAGCTAGGGTTTTGGCTATGTTTTGGAGATACCTATATTTATGCATATGCTAGATTATTATTTTCAATAAAGTTGGTTCATTTTGAATCAAATATATATTTTTCAATATTATGTCCTCCAGCCAATGCTTGTAGAAATCAATTAGATTAGTTTTAAATTTTATTTGATATTGTTTAGGTTCAGACTAAATTATATTTGATCGATCACATTGTTGCTTCTTTCAAAGATTGGACATGATTTCGCTTTTAAAGTTGCAATTTTTTTCAAATATCTTTGTGTGCCTAATCTTTGGTGATCTTATGTTGTTTCTTGGATTTCAATTTTATAGATTTTATATTCTGATTGTATATGCAGTGTATCATTTTTTTCTCTTTCAGAATGATATTTATTTTAGTTATGGTTTGTTAATTTGTTTCAGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTCCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAAGTAAGTTATGCAATTCCTTCAATCTTATATTATTGATACATGTATAACAAATTATAGTATTTTGATTGCAAAGTGTTTGATTTCAGTGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTGAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAAAGAAGCAATCATGACGCGCCTAATTCTGATAGTGGAAATGGGAAACATTCGGCTGGGACAGGAAATTCTGATAAAAATGAAAAGCCATCTAGAAAACGGAAGGATAAGAATGAAGATGATGGTGAGGAAGAAAGTGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCGCTGATTTACACCGCAAGTTTGTTGCTGCGGTTAATCAATTAGGCGTTGACAGTACGTTTTCCCTCTTTCTTCAATGCGCAAAAACTTGTCAGCATAATTTTTATCAATTGCTTATATTCGTCTTTTTCTTTCCTTTTTTCCAGTTTATCAAAATATTTGTGCAGATTATAGCAATCCAATGTATTATTTGAAGACTTCCTCAAATATTCTTTACTAAAAATACTAATGTTTAGCCATGCTACGAATTTTTCCGATTCTGAAGTGTTTTTCTCATGATAGTTGTTATCTTGCAGAGGCTGTACCGAAGAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAGAAGGTGGCCAGCCATCTCCAGGTACTTTTTCTTATCTCTTATGCTTTACCTTTAGAAGTTACGAAGCATCGACATAGACATGTAAACCTGTAACGAGCCTTACACATGGACACTGCTAATGGTTTGAATAAATGAAGTAATTGAATTTAACAACATATGGTGATTCGTGTTAGTGTCGGATGCAGAATGCTCAGTGTCGGTGCTACATAAATTATCAGTCTTTTCATTCAAGAAAACTTCCTGTTTTTTAATTTTTCTGCAGAAGTATAGACTCTATCTAAAAAGAATTAGTTGTGCGGAAGACAAACAAGATCATATGGCAGCAGCCATAGCTAGTGGTTCAGATACATCCTATTTAAGAAGTGGAGTTGGAGGACATTTGCATACATTGAATGGCTCTACACAGTTTCATAACCATTATAACAATCCTTTTAGATCCTTTCCATCTGGTGGTGTTATGAGTAGTAGATTCAACACACCTACTAATGTGAATATGAATGGATTGCATTCTGCCGGAACTCTTCAGCCGAGTCAGACACATAATTTAAACAACTTGACAGATGATCAGCTAAAGTTCCAATCGGCCCTAACTCGCGGCAATCCAAATGATGTTCAAAGAGGTATCTCTATTAGTCCTATCCAAAACATGACCAATAACCTCCCAAATTTCAGTTTCACAAACAATCCCTTGATGTTAGAAGGAAATTCTCAAGACAAACAAGTAGGTGAAGGATACAAAAATTTAGCATTGCAGAATTCACAGTTTCTCCCTTTACTTGAAAACAGAAGGTGCAATGGTATTTGGTCAAGCACAATGCAACAACTACCTGGAACAAACTCTTATCTTCCAAGAGAATCTTTTAACCATGCTGCTATGCCTCATGTGTCATTTCAAGGTTGGGATAATAACAATAATCATGATGGAAGTTACCATTCTTCTAATGTGATCGGCAGTTCAATAGGCTCTTCTATGATTCCTGGTATGAATGTTGTCGAGCAAGAAGGAAACTTGGACTACAATTATGGTGATTCGGTACAATGA
Protein sequence

>MS.gene016038.t1

MNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKHSAGTGNSDKNEKPSRKRKDKNEDDGEEESEDDYDNDDQTAQKKPRTVWSADLHRKFVAAVNQLGVDKAVPKKILELMNVENLTREKVASHLQKYRLYLKRISCAEDKQDHMAAAIASGSDTSYLRSGVGGHLHTLNGSTQFHNHYNNPFRSFPSGGVMSSRFNTPTNVNMNGLHSAGTLQPSQTHNLNNLTDDQLKFQSALTRGNPNDVQRGISISPIQNMTNNLPNFSFTNNPLMLEGNSQDKQVGEGYKNLALQNSQFLPLLENRRCNGIWSSTMQQLPGTNSYLPRESFNHAAMPHVSFQGWDNNNNHDGSYHSSNVIGSSIGSSMIPGMNVVEQEGNLDYNYGDSVQ