AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016140

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016140.t1 MTR_6g053640 59.794 97 39 0 281 377 38 134 1.98e-31 117
MS.gene016140.t1 MTR_6g025550 50.000 116 24 2 110 225 47 128 7.00e-29 110
MS.gene016140.t1 MTR_3g005220 60.976 82 28 2 2 83 48 125 8.33e-28 108
MS.gene016140.t1 MTR_6g024175 66.176 68 23 0 281 348 117 184 2.10e-24 99.8
MS.gene016140.t1 MTR_4g035820 38.889 126 71 3 166 291 327 446 1.46e-15 79.0
MS.gene016140.t1 MTR_2g098880 50.000 66 33 0 28 93 59 124 5.90e-14 71.6
MS.gene016140.t1 MTR_2g098880 50.000 66 33 0 28 93 71 136 7.97e-14 71.6
MS.gene016140.t1 MTR_6g022070 28.148 135 96 1 119 252 33 167 8.30e-13 69.3
MS.gene016140.t1 MTR_2g098920 38.095 84 48 1 5 84 51 134 2.64e-12 67.0
MS.gene016140.t1 MTR_8g465780 30.822 146 100 1 159 303 1 146 1.04e-11 63.9
MS.gene016140.t1 MTR_3g020080 36.559 93 38 2 142 230 7 82 2.99e-11 61.2
MS.gene016140.t1 MTR_6g060130 54.902 51 22 1 2 52 73 122 5.24e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016140.t1 AT4G38180 45.506 178 97 0 104 281 392 569 3.54e-48 175
MS.gene016140.t1 AT4G15090 27.797 295 154 3 104 345 368 656 9.97e-36 140
MS.gene016140.t1 AT4G15090 27.797 295 154 3 104 345 368 656 9.97e-36 140
MS.gene016140.t1 AT4G15090 27.797 295 154 3 104 345 368 656 9.97e-36 140
MS.gene016140.t1 AT4G15090 27.797 295 154 3 104 345 368 656 9.97e-36 140
MS.gene016140.t1 AT4G15090 27.797 295 154 3 104 345 368 656 9.97e-36 140
MS.gene016140.t1 AT4G38170 27.090 299 161 4 108 352 161 456 2.71e-35 137
MS.gene016140.t1 AT5G18960 43.836 146 79 1 118 263 503 645 7.31e-32 128
MS.gene016140.t1 AT5G18960 38.462 104 57 2 19 118 28 128 3.73e-13 71.6
MS.gene016140.t1 AT5G18960 43.836 146 79 1 118 263 503 645 7.31e-32 128
MS.gene016140.t1 AT5G18960 38.462 104 57 2 19 118 28 128 3.73e-13 71.6
MS.gene016140.t1 AT2G27110 34.483 174 112 1 108 281 343 514 1.20e-31 127
MS.gene016140.t1 AT2G27110 34.483 174 112 1 108 281 343 514 1.20e-31 127
MS.gene016140.t1 AT2G27110 34.483 174 112 1 108 281 198 369 1.26e-31 127
MS.gene016140.t1 AT3G22170 27.090 299 158 4 105 350 378 669 4.85e-29 120
MS.gene016140.t1 AT3G22170 27.090 299 158 4 105 350 378 669 4.85e-29 120
MS.gene016140.t1 AT3G06250 38.411 151 90 1 118 268 479 626 1.83e-28 118
MS.gene016140.t1 AT3G06250 39.130 115 64 2 7 118 2 113 8.98e-16 80.1
MS.gene016140.t1 AT3G06250 38.411 151 90 1 118 268 479 626 1.83e-28 118
MS.gene016140.t1 AT3G06250 39.130 115 64 2 7 118 2 113 8.98e-16 80.1
MS.gene016140.t1 AT3G06250 38.411 151 90 1 118 268 479 626 1.83e-28 118
MS.gene016140.t1 AT3G06250 39.130 115 64 2 7 118 2 113 8.98e-16 80.1
MS.gene016140.t1 AT1G76320 25.589 297 160 3 105 348 306 594 9.90e-25 107
MS.gene016140.t1 AT1G76320 25.589 297 160 3 105 348 306 594 1.03e-24 107
MS.gene016140.t1 AT1G76320 25.589 297 160 3 105 348 306 594 1.03e-24 107
MS.gene016140.t1 AT1G76320 25.589 297 160 3 105 348 306 594 1.12e-24 107
MS.gene016140.t1 AT1G52520 34.161 161 98 3 104 260 389 545 4.90e-24 105
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 256 418 2.11e-22 100
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 313 475 2.12e-22 100
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 313 475 2.12e-22 100
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 313 475 2.30e-22 100
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 327 489 2.50e-22 100
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 313 475 2.52e-22 100
MS.gene016140.t1 AT4G19990 30.675 163 113 0 101 263 313 475 2.52e-22 100
MS.gene016140.t1 AT1G10240 34.177 158 102 2 105 261 371 527 7.98e-22 98.2
MS.gene016140.t1 AT1G10240 34.177 158 102 2 105 261 371 527 7.98e-22 98.2
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 313 470 5.11e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 313 470 5.11e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 313 470 5.57e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 313 470 5.60e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 313 470 5.60e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 313 470 5.60e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 272 429 6.00e-20 92.8
MS.gene016140.t1 AT2G32250 32.704 159 105 2 105 262 272 429 6.12e-20 92.8
MS.gene016140.t1 AT1G80010 28.302 212 133 4 81 280 387 591 1.48e-19 91.7
MS.gene016140.t1 AT5G28530 30.872 149 100 2 134 281 352 498 1.27e-15 79.3
MS.gene016140.t1 AT5G28530 30.872 149 100 2 134 281 415 561 1.79e-15 79.0
MS.gene016140.t1 AT5G28530 30.201 149 97 2 134 281 352 494 5.90e-15 77.4
MS.gene016140.t1 AT5G28530 30.201 149 97 2 134 281 415 557 8.77e-15 76.6
MS.gene016140.t1 AT5G28530 30.201 149 97 2 134 281 415 557 8.77e-15 76.6

Find 72 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTTATACTTTCACTTCTT+TGG 0.136062 1.2:+7205797 None:intergenic
GAAAATGTTTAGAATATTTC+AGG 0.197001 1.2:-7205593 MS.gene016140:CDS
AATGGTAGTCCGTTATTTAT+AGG 0.222226 1.2:+7207026 None:intergenic
ATTGATAATTGAAGAGTTAC+AGG 0.225787 1.2:-7205287 MS.gene016140:CDS
TTGTTCAAAGGAAGGGTTTC+GGG 0.231331 1.2:-7206844 MS.gene016140:CDS
TTCATTGTTTAATAGCATTA+TGG 0.254555 1.2:-7205239 MS.gene016140:CDS
CATTCCTTCAAAGGGCTCTA+AGG 0.274189 1.2:+7206995 None:intergenic
ATTTATACTTTCACTTCTTT+GGG 0.306048 1.2:+7205798 None:intergenic
CTCTCTTTCCTTGTTGTAAC+GGG 0.308781 1.2:+7205699 None:intergenic
AGAACAGACGGAATGATAAT+TGG 0.340924 1.2:-7206879 MS.gene016140:CDS
TTTGTCGCAGAAGAAGTTAA+AGG 0.347626 1.2:-7205547 MS.gene016140:CDS
TATACTCCATTCGAGCAAAA+TGG 0.347854 1.2:-7205867 MS.gene016140:CDS
ATGTCAAGAGCAGCAGTTTC+AGG 0.356702 1.2:+7207053 None:intergenic
ATACTGATGTCAGACGCTTT+TGG 0.376889 1.2:+7205064 None:intergenic
TTTGCGAATGAAAAGCAGTT+TGG 0.393660 1.2:-7204993 MS.gene016140:CDS
CCTCCACTTGCGGCAACTAA+AGG 0.406674 1.2:-7205028 MS.gene016140:CDS
TAGAAGAATTTGATTCTGAA+TGG 0.406738 1.2:-7205942 MS.gene016140:CDS
ACTCCTCATTAGATTCTTCA+AGG 0.426629 1.2:+7205113 None:intergenic
TTTGATGCTTCTTCTTCCAT+TGG 0.427301 1.2:+7205628 None:intergenic
TCTCTCTTTCCTTGTTGTAA+CGG 0.435704 1.2:+7205698 None:intergenic
CTCCACTTGCGGCAACTAAA+GGG 0.437572 1.2:-7205027 MS.gene016140:CDS
ATATTTCAGGATGAATTAGT+TGG 0.438960 1.2:-7205580 MS.gene016140:CDS
TTTGTTCAAAGGAAGGGTTT+CGG 0.447784 1.2:-7206845 MS.gene016140:CDS
CCTTTAGTTGCCGCAAGTGG+AGG 0.451227 1.2:+7205028 None:intergenic
CGCCCTTTAGTTGCCGCAAG+TGG 0.458861 1.2:+7205025 None:intergenic
AGAAGAATTTGATTCTGAAT+GGG 0.459561 1.2:-7205941 MS.gene016140:CDS
GAAGCAATGATAGATACATA+TGG 0.475667 1.2:-7205919 MS.gene016140:CDS
CTTAGAGCCCTTTGAAGGAA+TGG 0.476103 1.2:-7206994 MS.gene016140:CDS
AGGCAAGGAATCACTACAAA+AGG 0.478706 1.2:-7206953 MS.gene016140:CDS
GGATATAAAGTTCGCAAAAG+TGG 0.480862 1.2:+7205649 None:intergenic
ATTCCTTCAAAGGGCTCTAA+GGG 0.488264 1.2:+7206996 None:intergenic
CCTTGTTGTAACGGGCATCA+AGG 0.494322 1.2:+7205707 None:intergenic
TGTGAACTCCATTCCTTCAA+AGG 0.507742 1.2:+7206986 None:intergenic
CACAGTATGTTCTACAGAGA+TGG 0.508769 1.2:-7205342 MS.gene016140:intron
TTCAGAATCAAATTCTTCTA+CGG 0.510630 1.2:+7205945 None:intergenic
TTTGCGAACTTTATATCCAA+TGG 0.514843 1.2:-7205644 MS.gene016140:CDS
GTGAACTCCATTCCTTCAAA+GGG 0.518109 1.2:+7206987 None:intergenic
TGTGTCACAAACTTGCTCAA+TGG 0.518813 1.2:+7205742 None:intergenic
CTTGTTGTAACGGGCATCAA+GGG 0.519566 1.2:+7205708 None:intergenic
GTGATTCCTTGCCTCTTCGA+TGG 0.520594 1.2:+7206962 None:intergenic
TAAAGAGTTTCTTTGTTCAA+AGG 0.523442 1.2:-7206856 MS.gene016140:CDS
GACATGGATCCTATAAATAA+CGG 0.523874 1.2:-7207035 MS.gene016140:CDS
GAGTTTCTTTGTTCAAAGGA+AGG 0.524715 1.2:-7206852 MS.gene016140:CDS
GTGTCAGACATGATGAAACT+AGG 0.525439 1.2:-7206788 MS.gene016140:CDS
AAATCATGACGTACATAAGC+AGG 0.530663 1.2:+7205841 None:intergenic
AGTTTCTTTGTTCAAAGGAA+GGG 0.533222 1.2:-7206851 MS.gene016140:CDS
ATGGGTTGCAAGATAATCAA+TGG 0.535566 1.2:-7205900 MS.gene016140:CDS
TATACTTTCACTTCTTTGGG+TGG 0.539289 1.2:+7205801 None:intergenic
TCACTTGAACTAGCTGAAAG+AGG 0.543111 1.2:-7205211 MS.gene016140:CDS
ATTGAAGAGTTACAGGAAGG+AGG 0.545024 1.2:-7205280 MS.gene016140:CDS
AAGCAATGATAGATACATAT+GGG 0.546295 1.2:-7205918 MS.gene016140:CDS
CTTTAGTTGCCGCAAGTGGA+GGG 0.548275 1.2:+7205029 None:intergenic
TAGTGAAGTGTCAACATACA+AGG 0.553752 1.2:-7205521 MS.gene016140:intron
ATTCTTCTACGGTCGTACAT+AGG 0.554757 1.2:+7205956 None:intergenic
TAGACATGATTCAAGTACTC+TGG 0.558386 1.2:+7206024 None:intergenic
AACTAGCTGAAAGAGGTTCG+CGG 0.560322 1.2:-7205204 MS.gene016140:CDS
TTTGCATGACCCTCCACTTG+CGG 0.565228 1.2:-7205038 MS.gene016140:CDS
TGAAACTGCTGCTCTTGACA+TGG 0.571525 1.2:-7207051 MS.gene016140:CDS
ATAATTGAAGAGTTACAGGA+AGG 0.574561 1.2:-7205283 MS.gene016140:CDS
CTGTTCTTGATTTAGTAACA+CGG 0.577898 1.2:+7206895 None:intergenic
AGTAACACGGCCCATGCGAA+AGG 0.583261 1.2:+7206908 None:intergenic
AAGCATCAAAAGTTTATACA+AGG 0.598083 1.2:-7205615 MS.gene016140:CDS
TGTCAGACATGATGAAACTA+GGG 0.602315 1.2:-7206787 MS.gene016140:intron
CCTTGATGCCCGTTACAACA+AGG 0.607670 1.2:-7205707 MS.gene016140:CDS
GAGCCTTGAAGAATCTAATG+AGG 0.612129 1.2:-7205116 MS.gene016140:CDS
GTTACTAAATCAAGAACAGA+CGG 0.612323 1.2:-7206891 MS.gene016140:CDS
TACATCATCAGAACAAGCCA+TGG 0.620956 1.2:+7207076 None:intergenic
TCACATCCATCGAAGAGGCA+AGG 0.624553 1.2:-7206968 MS.gene016140:CDS
AAAAGGTACGCTAAGAACAA+AGG 0.638535 1.2:-7206936 MS.gene016140:CDS
TCCTTCAAAGGGCTCTAAGG+GGG 0.640867 1.2:+7206998 None:intergenic
TTCCTTCAAAGGGCTCTAAG+GGG 0.673359 1.2:+7206997 None:intergenic
GGAGTTCACATCCATCGAAG+AGG 0.673976 1.2:-7206973 MS.gene016140:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAAAATTGATAGAT+AGG + chr1.2:7206885-7206904 None:intergenic 10.0%
!! TATTTAAAAAAAAGTGAAGA+TGG - chr1.2:7205000-7205019 MS.gene016140:CDS 15.0%
!!! TTTTTCTTTCTTAACATTAA+GGG + chr1.2:7206429-7206448 None:intergenic 15.0%
!! ATTTATACTTTCACTTCTTT+GGG + chr1.2:7205949-7205968 None:intergenic 20.0%
!! GTTAAGAAAGAAAAAGTAAA+AGG - chr1.2:7206434-7206453 MS.gene016140:intron 20.0%
!! TAAATTTAGACATTTACGTT+TGG + chr1.2:7206831-7206850 None:intergenic 20.0%
!! TATTTATACTTTCACTTCTT+TGG + chr1.2:7205950-7205969 None:intergenic 20.0%
!! TATTTATTTGTCAAGTATGT+CGG + chr1.2:7205450-7205469 None:intergenic 20.0%
!! TTAATCATCATTATTGTATG+TGG - chr1.2:7205679-7205698 MS.gene016140:CDS 20.0%
!! TTCATTGTTTAATAGCATTA+TGG - chr1.2:7206505-7206524 MS.gene016140:intron 20.0%
!!! AAAATGGCATACAAATATTT+TGG - chr1.2:7205405-7205424 MS.gene016140:intron 20.0%
!!! AAAATTAGCAAATTGCTTTT+GGG - chr1.2:7205368-7205387 MS.gene016140:intron 20.0%
!!! CAATTGACAATTTTTAAAGT+AGG + chr1.2:7205304-7205323 None:intergenic 20.0%
!!! CTTTTTCTTTCTTAACATTA+AGG + chr1.2:7206430-7206449 None:intergenic 20.0%
!!! GAAAATGTTTAGAATATTTC+AGG - chr1.2:7206151-7206170 MS.gene016140:intron 20.0%
!!! GAGAATAAACATTTTTCATA+AGG + chr1.2:7206376-7206395 None:intergenic 20.0%
! AAAAAAAAGTGAAGATGGAT+GGG - chr1.2:7205005-7205024 MS.gene016140:CDS 25.0%
! AAGCAATGATAGATACATAT+GGG - chr1.2:7205826-7205845 MS.gene016140:CDS 25.0%
! AAGCATCAAAAGTTTATACA+AGG - chr1.2:7206129-7206148 MS.gene016140:intron 25.0%
! AATAACATCTGTCTTATGTA+CGG - chr1.2:7205170-7205189 MS.gene016140:CDS 25.0%
! AGAAGAATTTGATTCTGAAT+GGG - chr1.2:7205803-7205822 MS.gene016140:CDS 25.0%
! AGACAAAAGCAATTAGAAAA+AGG - chr1.2:7205258-7205277 MS.gene016140:CDS 25.0%
! ATATTTCAGGATGAATTAGT+TGG - chr1.2:7206164-7206183 MS.gene016140:intron 25.0%
! CAATAATGATGATTAACCTT+TGG + chr1.2:7205675-7205694 None:intergenic 25.0%
! GAGAAAATAGTACATGATTA+TGG + chr1.2:7205053-7205072 None:intergenic 25.0%
! GTCTAAATTTAAATCAGACA+AGG - chr1.2:7206840-7206859 MS.gene016140:CDS 25.0%
! TAAAAAAAAGTGAAGATGGA+TGG - chr1.2:7205004-7205023 MS.gene016140:CDS 25.0%
! TAAAGAGTTTCTTTGTTCAA+AGG - chr1.2:7204888-7204907 MS.gene016140:intron 25.0%
! TAGAAGAATTTGATTCTGAA+TGG - chr1.2:7205802-7205821 MS.gene016140:CDS 25.0%
! TTCAGAATCAAATTCTTCTA+CGG + chr1.2:7205802-7205821 None:intergenic 25.0%
!! ATTGATAATTGAAGAGTTAC+AGG - chr1.2:7206457-7206476 MS.gene016140:intron 25.0%
!! CAAAGAAATTGACAACTTTT+AGG + chr1.2:7205075-7205094 None:intergenic 25.0%
!! CATCAACAATCTATTCTTTT+TGG - chr1.2:7205507-7205526 MS.gene016140:intron 25.0%
!! GAAAATTAGCAAATTGCTTT+TGG - chr1.2:7205367-7205386 MS.gene016140:intron 25.0%
!! GTTTTTCTATGCATTTCAAA+TGG - chr1.2:7205335-7205354 MS.gene016140:CDS 25.0%
!!! AAACTAAAGAGAGTTTTGTT+TGG - chr1.2:7205550-7205569 MS.gene016140:CDS 25.0%
!!! ATACAAATATTTTGGTGATG+TGG - chr1.2:7205413-7205432 MS.gene016140:intron 25.0%
!!! TGTCACAAGTTTGATTTTTT+GGG - chr1.2:7206320-7206339 MS.gene016140:intron 25.0%
!!! TTCTAGTTTGATTGACTAAT+AGG - chr1.2:7206988-7207007 MS.gene016140:CDS 25.0%
!!! TTGTCACAAGTTTGATTTTT+TGG - chr1.2:7206319-7206338 MS.gene016140:intron 25.0%
AATATCAGACCTAAAATGCA+GGG + chr1.2:7206932-7206951 None:intergenic 30.0%
AATGGTAGTCCGTTATTTAT+AGG + chr1.2:7204721-7204740 None:intergenic 30.0%
AATTGTCAATTGAAGAATCC+TGG - chr1.2:7205312-7205331 MS.gene016140:CDS 30.0%
AGTTTCTTTGTTCAAAGGAA+GGG - chr1.2:7204893-7204912 MS.gene016140:intron 30.0%
ATAATTGAAGAGTTACAGGA+AGG - chr1.2:7206461-7206480 MS.gene016140:intron 30.0%
ATTGTCAATTGAAGAATCCT+GGG - chr1.2:7205313-7205332 MS.gene016140:CDS 30.0%
CTGTTCTTGATTTAGTAACA+CGG + chr1.2:7204852-7204871 None:intergenic 30.0%
GAAGCAATGATAGATACATA+TGG - chr1.2:7205825-7205844 MS.gene016140:CDS 30.0%
GACATGGATCCTATAAATAA+CGG - chr1.2:7204709-7204728 MS.gene016140:intron 30.0%
GTTACTAAATCAAGAACAGA+CGG - chr1.2:7204853-7204872 MS.gene016140:intron 30.0%
TCAAAAGTCACATATTAGCT+AGG + chr1.2:7206272-7206291 None:intergenic 30.0%
TGTAGTTCCTGTAAGAATAA+GGG - chr1.2:7206800-7206819 MS.gene016140:CDS 30.0%
TTGTAGTTCCTGTAAGAATA+AGG - chr1.2:7206799-7206818 MS.gene016140:CDS 30.0%
TTTGCGAACTTTATATCCAA+TGG - chr1.2:7206100-7206119 MS.gene016140:intron 30.0%
! CGCAATATCATGATGTTTTT+CGG + chr1.2:7206567-7206586 None:intergenic 30.0%
!! TTTTGGTTGTGCTTTGTTAT+GGG - chr1.2:7205524-7205543 MS.gene016140:CDS 30.0%
!!! AAGACAGATGTTATTTTGCT+AGG + chr1.2:7205165-7205184 None:intergenic 30.0%
!!! TGATCTGTGATAATGGTTTT+AGG + chr1.2:7205615-7205634 None:intergenic 30.0%
!!! TTTTTGGTTGTGCTTTGTTA+TGG - chr1.2:7205523-7205542 MS.gene016140:CDS 30.0%
AAATCATGACGTACATAAGC+AGG + chr1.2:7205906-7205925 None:intergenic 35.0%
ACCATCTTGATCTGTGATAA+TGG + chr1.2:7205622-7205641 None:intergenic 35.0%
ACCATTATCACAGATCAAGA+TGG - chr1.2:7205618-7205637 MS.gene016140:CDS 35.0%
ACTCCTCATTAGATTCTTCA+AGG + chr1.2:7206634-7206653 None:intergenic 35.0%
AGAACAGACGGAATGATAAT+TGG - chr1.2:7204865-7204884 MS.gene016140:intron 35.0%
ATGATGATTAACTCCAGTGA+AGG + chr1.2:7205490-7205509 None:intergenic 35.0%
CAATATCAGACCTAAAATGC+AGG + chr1.2:7206933-7206952 None:intergenic 35.0%
GAGTTTCTTTGTTCAAAGGA+AGG - chr1.2:7204892-7204911 MS.gene016140:intron 35.0%
GCATTTCAAATGGATGCTAA+AGG - chr1.2:7205345-7205364 MS.gene016140:intron 35.0%
GGATATAAAGTTCGCAAAAG+TGG + chr1.2:7206098-7206117 None:intergenic 35.0%
TAGACATGATTCAAGTACTC+TGG + chr1.2:7205723-7205742 None:intergenic 35.0%
TATACTCCATTCGAGCAAAA+TGG - chr1.2:7205877-7205896 MS.gene016140:CDS 35.0%
TATACTTTCACTTCTTTGGG+TGG + chr1.2:7205946-7205965 None:intergenic 35.0%
TCTGTAGAACATACTGTGAT+GGG + chr1.2:7206400-7206419 None:intergenic 35.0%
TGAAATGCATAGAAAAACCC+AGG + chr1.2:7205333-7205352 None:intergenic 35.0%
TGATGATTAACTCCAGTGAA+GGG + chr1.2:7205489-7205508 None:intergenic 35.0%
TGTCAGACATGATGAAACTA+GGG - chr1.2:7204957-7204976 MS.gene016140:intron 35.0%
TTTGATGCTTCTTCTTCCAT+TGG + chr1.2:7206119-7206138 None:intergenic 35.0%
TTTGCGAATGAAAAGCAGTT+TGG - chr1.2:7206751-7206770 MS.gene016140:intron 35.0%
TTTGTCGCAGAAGAAGTTAA+AGG - chr1.2:7206197-7206216 MS.gene016140:intron 35.0%
TTTGTTCAAAGGAAGGGTTT+CGG - chr1.2:7204899-7204918 MS.gene016140:intron 35.0%
! AAAAGGTACGCTAAGAACAA+AGG - chr1.2:7204808-7204827 MS.gene016140:intron 35.0%
! AAAGGTTTTACCTTTCGCAT+GGG - chr1.2:7204826-7204845 MS.gene016140:intron 35.0%
! ATGGGTTGCAAGATAATCAA+TGG - chr1.2:7205844-7205863 MS.gene016140:CDS 35.0%
! GGTTGATTCAAGGTCTAAAA+TGG - chr1.2:7205389-7205408 MS.gene016140:intron 35.0%
! TAGTGAAGTGTCAACATACA+AGG - chr1.2:7206223-7206242 MS.gene016140:intron 35.0%
! TCTCTCTTTCCTTGTTGTAA+CGG + chr1.2:7206049-7206068 None:intergenic 35.0%
!!! ATTGCTTTTGGGTTGATTCA+AGG - chr1.2:7205379-7205398 MS.gene016140:intron 35.0%
ACTTGGTTAGAAGCAATGGA+TGG - chr1.2:7205582-7205601 MS.gene016140:CDS 40.0%
AGACATGATGAAACTAGGGT+AGG - chr1.2:7204961-7204980 MS.gene016140:intron 40.0%
AGGCAAGGAATCACTACAAA+AGG - chr1.2:7204791-7204810 MS.gene016140:intron 40.0%
ATTCTTCTACGGTCGTACAT+AGG + chr1.2:7205791-7205810 None:intergenic 40.0%
ATTGAAGAGTTACAGGAAGG+AGG - chr1.2:7206464-7206483 MS.gene016140:intron 40.0%
ATTGATGTGCTGCATGATTC+TGG - chr1.2:7205132-7205151 MS.gene016140:CDS 40.0%
CACAGTATGTTCTACAGAGA+TGG - chr1.2:7206402-7206421 MS.gene016140:intron 40.0%
CTCTGTAGAACATACTGTGA+TGG + chr1.2:7206401-7206420 None:intergenic 40.0%
GAGCCTTGAAGAATCTAATG+AGG - chr1.2:7206628-7206647 MS.gene016140:intron 40.0%
GGTATGACCCTTATTCTTAC+AGG + chr1.2:7206810-7206829 None:intergenic 40.0%
GTGAACTCCATTCCTTCAAA+GGG + chr1.2:7204760-7204779 None:intergenic 40.0%
GTGTCAGACATGATGAAACT+AGG - chr1.2:7204956-7204975 MS.gene016140:intron 40.0%
TCACTTGAACTAGCTGAAAG+AGG - chr1.2:7206533-7206552 MS.gene016140:intron 40.0%
TCTGTCTTATGTACGGAAAG+TGG - chr1.2:7205177-7205196 MS.gene016140:CDS 40.0%
TGTGAACTCCATTCCTTCAA+AGG + chr1.2:7204761-7204780 None:intergenic 40.0%
TTGTTCAAAGGAAGGGTTTC+GGG - chr1.2:7204900-7204919 MS.gene016140:intron 40.0%
! AGATGGTTCTATCACGTATG+CGG - chr1.2:7205635-7205654 MS.gene016140:CDS 40.0%
! AGGAATCCATTTTGCTCGAA+TGG + chr1.2:7205886-7205905 None:intergenic 40.0%
! AGTTTGGAGTTGCGCAAAAA+AGG - chr1.2:7206767-7206786 MS.gene016140:intron 40.0%
! ATACTGATGTCAGACGCTTT+TGG + chr1.2:7206683-7206702 None:intergenic 40.0%
! ATTCCTTCAAAGGGCTCTAA+GGG + chr1.2:7204751-7204770 None:intergenic 40.0%
! CAAAGGTTTTACCTTTCGCA+TGG - chr1.2:7204825-7204844 MS.gene016140:intron 40.0%
! CTCTCTTTCCTTGTTGTAAC+GGG + chr1.2:7206048-7206067 None:intergenic 40.0%
! CTTTTCATTCGCAAAGTTCG+TGG + chr1.2:7206746-7206765 None:intergenic 40.0%
! GTACGTCATGATTTTTGTGC+TGG - chr1.2:7205912-7205931 MS.gene016140:CDS 40.0%
! TCTCTAACACCCTGCATTTT+AGG - chr1.2:7206920-7206939 MS.gene016140:CDS 40.0%
! TGTGTCACAAACTTGCTCAA+TGG + chr1.2:7206005-7206024 None:intergenic 40.0%
!! AGTGGTGGTGTTGATAACAT+TGG - chr1.2:7205195-7205214 MS.gene016140:CDS 40.0%
AACTAGCTGAAAGAGGTTCG+CGG - chr1.2:7206540-7206559 MS.gene016140:intron 45.0%
ACTCCAGTGAAGGGAACAAA+CGG + chr1.2:7205480-7205499 None:intergenic 45.0%
ATGTCAAGAGCAGCAGTTTC+AGG + chr1.2:7204694-7204713 None:intergenic 45.0%
CATTCCTTCAAAGGGCTCTA+AGG + chr1.2:7204752-7204771 None:intergenic 45.0%
GCGTACTTGGTTAGAAGCAA+TGG - chr1.2:7205578-7205597 MS.gene016140:CDS 45.0%
GTCTTATGTACGGAAAGTGG+TGG - chr1.2:7205180-7205199 MS.gene016140:CDS 45.0%
TGAAACTAGGGTAGGATGCA+AGG - chr1.2:7204969-7204988 MS.gene016140:intron 45.0%
TGAAACTGCTGCTCTTGACA+TGG - chr1.2:7204693-7204712 MS.gene016140:intron 45.0%
TTGTTTGGTTGCTGCGTACT+TGG - chr1.2:7205565-7205584 MS.gene016140:CDS 45.0%
! CTTAGAGCCCTTTGAAGGAA+TGG - chr1.2:7204750-7204769 MS.gene016140:intron 45.0%
!! CTTGTTGTAACGGGCATCAA+GGG + chr1.2:7206039-7206058 None:intergenic 45.0%
!! GTGCTGCATGATTCTGGTTT+AGG - chr1.2:7205138-7205157 MS.gene016140:CDS 45.0%
!! TTCCTTCAAAGGGCTCTAAG+GGG + chr1.2:7204750-7204769 None:intergenic 45.0%
!! ATTTATTATCAAAATTAAAA+AGG + chr1.2:7207047-7207066 None:intergenic 5.0%
!!! AAGTATAAATAATTTTTTTA+AGG - chr1.2:7205959-7205978 MS.gene016140:CDS 5.0%
CCTTGATGCCCGTTACAACA+AGG - chr1.2:7206037-7206056 MS.gene016140:intron 50.0%
CTCCACTTGCGGCAACTAAA+GGG - chr1.2:7206717-7206736 MS.gene016140:intron 50.0%
CTTTAGTTGCCGCAAGTGGA+GGG + chr1.2:7206718-7206737 None:intergenic 50.0%
GGAGTTCACATCCATCGAAG+AGG - chr1.2:7204771-7204790 MS.gene016140:intron 50.0%
GTGATTCCTTGCCTCTTCGA+TGG + chr1.2:7204785-7204804 None:intergenic 50.0%
TCACATCCATCGAAGAGGCA+AGG - chr1.2:7204776-7204795 MS.gene016140:intron 50.0%
TTTGCATGACCCTCCACTTG+CGG - chr1.2:7206706-7206725 MS.gene016140:intron 50.0%
! ATGCCGTTTGTTCCCTTCAC+TGG - chr1.2:7205474-7205493 MS.gene016140:intron 50.0%
!! CCTTGTTGTAACGGGCATCA+AGG + chr1.2:7206040-7206059 None:intergenic 50.0%
!! TCCTTCAAAGGGCTCTAAGG+GGG + chr1.2:7204749-7204768 None:intergenic 50.0%
AGTAACACGGCCCATGCGAA+AGG + chr1.2:7204839-7204858 None:intergenic 55.0%
CCTCCACTTGCGGCAACTAA+AGG - chr1.2:7206716-7206735 MS.gene016140:intron 55.0%
CCTTTAGTTGCCGCAAGTGG+AGG + chr1.2:7206719-7206738 None:intergenic 55.0%
TATCACGTATGCGGTCGCGA+AGG - chr1.2:7205644-7205663 MS.gene016140:CDS 55.0%
TCCCCCTTAGAGCCCTTTGA+AGG - chr1.2:7204745-7204764 MS.gene016140:intron 55.0%
!! GGCTCTAAGGGGGACTCTAA+TGG + chr1.2:7204739-7204758 None:intergenic 55.0%
!! GGTCGCGAAGGTGTTTCCAA+AGG - chr1.2:7205656-7205675 MS.gene016140:CDS 55.0%
CGCCCTTTAGTTGCCGCAAG+TGG + chr1.2:7206722-7206741 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 7204670 7207096 7204670 ID=MS.gene016140
chr1.2 mRNA 7204670 7207096 7204670 ID=MS.gene016140.t1;Parent=MS.gene016140
chr1.2 exon 7206788 7207096 7206788 ID=MS.gene016140.t1.exon1;Parent=MS.gene016140.t1
chr1.2 CDS 7206788 7207096 7206788 ID=cds.MS.gene016140.t1;Parent=MS.gene016140.t1
chr1.2 exon 7205522 7206055 7205522 ID=MS.gene016140.t1.exon2;Parent=MS.gene016140.t1
chr1.2 CDS 7205522 7206055 7205522 ID=cds.MS.gene016140.t1;Parent=MS.gene016140.t1
chr1.2 exon 7204978 7205359 7204978 ID=MS.gene016140.t1.exon3;Parent=MS.gene016140.t1
chr1.2 CDS 7204978 7205359 7204978 ID=cds.MS.gene016140.t1;Parent=MS.gene016140.t1
chr1.2 exon 7204670 7204677 7204670 ID=MS.gene016140.t1.exon4;Parent=MS.gene016140.t1
chr1.2 CDS 7204670 7204677 7204670 ID=cds.MS.gene016140.t1;Parent=MS.gene016140.t1
Gene Sequence

>MS.gene016140

ATGGCTTGTTCTGATGATGTACCTGAAACTGCTGCTCTTGACATGGATCCTATAAATAACGGACTACCATTAGAGTCCCCCTTAGAGCCCTTTGAAGGAATGGAGTTCACATCCATCGAAGAGGCAAGGAATCACTACAAAAGGTACGCTAAGAACAAAGGTTTTACCTTTCGCATGGGCCGTGTTACTAAATCAAGAACAGACGGAATGATAATTGGTAAAGAGTTTCTTTGTTCAAAGGAAGGGTTTCGGGCAAAAAAATCTGCAAAGAAAGAAAATAGCAGTCGTGTCAGACATGATGAAACTAGGGTAGGATGCAAGGCATTGTTATATTTAAAAAAAAGTGAAGATGGATGGGTTGTTTCAAGATTTTTAAGAGACCATAATCATGTACTATTTTCTCCTAAAAGTTGTCAATTTCTTTGAGTACATAGACAGAAAACAAAAGTGCAAAAAAAACTTATTGATGTGCTGCATGATTCTGGTTTAGGTCCTAGCAAAATAACATCTGTCTTATGTACGGAAAGTGGTGGTGTTGATAACATTGGATTTAGTCAACAAGATGTTATTAATTACTTATCTGAAAAGAGACAAAAGCAATTAGAAAAAGGAGATGCTCAAATAATGTTATCCTACTTTAAAAATTGTCAATTGAAGAATCCTGGGTTTTTCTATGCATTTCAAATGGATGCTAAAGGAAAATTAGCAAATTGCTTTTGGGTTGATTCAAGGTCTAAAATGGCATACAAATATTTTGGTGATGTGGTGACTTTTGATCCGACATACTTGACAAATAAATACAAAATGCCGTTTGTTCCCTTCACTGGAGTTAATCATCATCAACAATCTATTCTTTTTGGTTGTGCTTTGTTATGGGATGAAACTAAAGAGAGTTTTGTTTGGTTGCTGCGTACTTGGTTAGAAGCAATGGATGGAGTTTGTCCTAAAACCATTATCACAGATCAAGATGGTTCTATCACGTATGCGGTCGCGAAGGTGTTTCCAAAGGTTAATCATCATTATTGTATGTGGCATATTCAGAAAAAAGTTCCAGAGTACTTGAATCATGTCTATCACGAACATAGTGAATTTAAAAATCAGTTTTACAAGTGCGTTCACCTATGTACGACCGTAGAAGAATTTGATTCTGAATGGGAAGCAATGATAGATACATATGGGTTGCAAGATAATCAATGGTTAAAGACGATATACTCCATTCGAGCAAAATGGATTCCTGCTTATGTACGTCATGATTTTTGTGCTGGAATGTCTACCACCCAAAGAAGTGAAAGTATAAATAATTTTTTTAAGGACTTCTTGAATTCAAGTACTCCATTGAGCAAGTTTGTGACACAATATGATAAAGCCCTTGATGCCCGTTACAACAAGGAAAGAGAGAAGACTTTCAAGACAAGAAATTCAAAGCCACTTTTGCGAACTTTATATCCAATGGAAGAAGAAGCATCAAAAGTTTATACAAGGAAAATGTTTAGAATATTTCAGGATGAATTAGTTGGCTCTCAAATATTTGTCGCAGAAGAAGTTAAAGGTTCTAGTGAAGTGTCAACATACAAGGTTCATGAATTTTATAAAGAGAAGCCTAGCTAATATGTGACTTTTGATGTTACTTCAAACGAAGCAACTTGTAGTTGTCACAAGTTTGATTTTTTGGGTATTCTTTGTAGACATGTCTTAAGTGTATTCCTTATGAAAAATGTTTATTCTCTCCCATCACAGTATGTTCTACAGAGATGGACCCTTAATGTTAAGAAAGAAAAAGTAAAAGGATTGATAATTGAAGAGTTACAGGAAGGAGGAAACAAAGCTTCAAGCACTTCATTGTTTAATAGCATTATGGCTCACTCACTTGAACTAGCTGAAAGAGGTTCGCGGTCCGAAAAACATCATGATATTGCGATTGAATGTCTGAACAGTGCAGTTGCAAAACTTGATTTATTGAGCCTTGAAGAATCTAATGAGGAGTTTGTTAGTTCAACATCTGAAGTTATGCCAAAAGCGTCTGACATCAGTATAACTTTGCATGACCCTCCACTTGCGGCAACTAAAGGGCGTCCACGAACTTTGCGAATGAAAAGCAGTTTGGAGTTGCGCAAAAAAGGTTCATCTACTTGTAGTTCCTGTAAGAATAAGGGTCATACCAAACGTAAATGTCTAAATTTAAATCAGACAAGGTAGCTTAAAATATACATGTCCTATCTATCAATTTTATTTTTTCGATGTTAATTTGATTCTCTAACACCCTGCATTTTAGGTCTGATATTGTAAGCAATCAATTTTCAAACTCTAATTGCTGAAGCTTCTAGTTTGATTGACTAATAGGTAATAATTTTTCTTTTAATGTTACTAATAATAACCTTTTTAATTTTGATAATAAATTGTCTTCATATTCTAATTGCAGACCTTTGA
Protein sequence

>MS.gene016140.t1

MACSDDVPETAALDMDPINNGLPLESPLEPFEGMEFTSIEEARNHYKRYAKNKGFTFRMGRVTKSRTDGMIIGKEFLCSKEGFRAKKSAKKENSSRVRHDETRKKVPEYLNHVYHEHSEFKNQFYKCVHLCTTVEEFDSEWEAMIDTYGLQDNQWLKTIYSIRAKWIPAYVRHDFCAGMSTTQRSESINNFFKDFLNSSTPLSKFVTQYDKALDARYNKEREKTFKTRNSKPLLRTLYPMEEEASKVYTRKMFRIFQDELVGSQIFVAEEVKGSSEVSTYKYVLQRWTLNVKKEKVKGLIIEELQEGGNKASSTSLFNSIMAHSLELAERGSRSEKHHDIAIECLNSAVAKLDLLSLEESNEEFVSSTSEVMPKASDISITLHDPPLAATKGRPRTLRMKSSLELRKKDL