AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016155

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016155.t1 MTR_1g015185 78.824 85 14 1 1 81 179 263 6.78e-37 124
MS.gene016155.t1 MTR_8g019520 88.333 60 6 1 23 81 198 257 3.59e-34 117
MS.gene016155.t1 MTR_8g019520 88.333 60 6 1 23 81 197 256 3.71e-34 117
MS.gene016155.t1 MTR_2g099500 87.931 58 6 1 24 80 193 250 2.76e-31 109
MS.gene016155.t1 MTR_2g040990 83.929 56 7 1 28 81 198 253 1.21e-29 105
MS.gene016155.t1 MTR_4g015830 67.089 79 25 1 4 81 162 240 6.89e-29 103
MS.gene016155.t1 MTR_4g134510 81.132 53 10 0 28 80 202 254 3.27e-28 102
MS.gene016155.t1 MTR_7g068590 75.000 56 14 0 26 81 187 242 6.36e-27 98.2
MS.gene016155.t1 MTR_8g066480 54.545 55 12 2 28 81 104 146 1.75e-14 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016155.t1 AT5G20510 88.136 59 7 0 23 81 161 219 3.05e-36 121
MS.gene016155.t1 AT5G20510 88.136 59 7 0 23 81 202 260 6.03e-36 122
MS.gene016155.t1 AT3G42790 91.379 58 5 0 23 80 192 249 2.25e-35 120
MS.gene016155.t1 AT5G26210 89.091 55 6 0 26 80 200 254 1.29e-31 110
MS.gene016155.t1 AT2G02470 83.636 55 9 0 27 81 202 256 6.98e-31 108
MS.gene016155.t1 AT2G02470 83.636 55 9 0 27 81 193 247 7.67e-31 108
MS.gene016155.t1 AT5G05610 59.740 77 31 0 4 80 164 240 2.04e-28 102
MS.gene016155.t1 AT5G05610 59.740 77 31 0 4 80 164 240 2.04e-28 102
MS.gene016155.t1 AT3G11200 68.966 58 18 0 23 80 188 245 2.04e-26 97.1
MS.gene016155.t1 AT3G11200 68.966 58 18 0 23 80 175 232 2.28e-26 96.7
MS.gene016155.t1 AT1G14510 75.000 52 13 0 27 78 197 248 8.35e-26 95.9

Find 21 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTATGCAGCTGATGAGTTC+TGG 0.348702 1.2:+7039410 MS.gene016155:CDS
TTGATATGCTCAGCCCTTGC+CGG 0.382099 1.2:-7039487 None:intergenic
GCAGTGACATTTGCGAGAAA+TGG 0.391588 1.2:+7039437 MS.gene016155:CDS
GTCAAAGGACGAGGAGGTTG+AGG 0.459920 1.2:+7039327 MS.gene016155:CDS
GCTCTCTTGTTGCTGCAAGC+TGG 0.467608 1.2:-7039523 None:intergenic
ATTTGCGAGAAATGGTTTCA+CGG 0.533914 1.2:+7039445 MS.gene016155:CDS
CGTGAAGATTACTCCGGCAA+GGG 0.576974 1.2:+7039474 MS.gene016155:CDS
GGTTGAGGATGGTGTAGGTG+TGG 0.578977 1.2:+7039342 MS.gene016155:CDS
GAGGAGGTTGAGGATGGTGT+AGG 0.579309 1.2:+7039337 MS.gene016155:CDS
GCGTGAAGATTACTCCGGCA+AGG 0.589663 1.2:+7039473 MS.gene016155:CDS
GGCAATGCAAATGAAGTCAA+AGG 0.599376 1.2:+7039312 MS.gene016155:CDS
GCAAATGAAGTCAAAGGACG+AGG 0.610740 1.2:+7039318 MS.gene016155:CDS
GAGACTTTGTGTGGGGCATG+TGG 0.613065 1.2:+7039382 MS.gene016155:CDS
AAGGACGAGGAGGTTGAGGA+TGG 0.620188 1.2:+7039331 MS.gene016155:CDS
AACACGGAGAGACTTTGTGT+GGG 0.622939 1.2:+7039374 MS.gene016155:CDS
GGATGGTGTAGGTGTGGACG+AGG 0.628142 1.2:+7039348 MS.gene016155:CDS
GAACACGGAGAGACTTTGTG+TGG 0.630911 1.2:+7039373 MS.gene016155:CDS
ACACGGAGAGACTTTGTGTG+GGG 0.707279 1.2:+7039375 MS.gene016155:CDS
AATGAAGTCAAAGGACGAGG+AGG 0.714948 1.2:+7039321 MS.gene016155:CDS
GGTGTGGACGAGGAAGAACA+CGG 0.729144 1.2:+7039358 MS.gene016155:CDS
AAAATGCGTGAAGATTACTC+CGG 0.732353 1.2:+7039468 MS.gene016155:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AAAATGCGTGAAGATTACTC+CGG + chr1.2:7039468-7039487 MS.gene016155:CDS 35.0%
ATTTGCGAGAAATGGTTTCA+CGG + chr1.2:7039445-7039464 MS.gene016155:CDS 35.0%
GGCAATGCAAATGAAGTCAA+AGG + chr1.2:7039312-7039331 MS.gene016155:CDS 40.0%
AACACGGAGAGACTTTGTGT+GGG + chr1.2:7039374-7039393 MS.gene016155:CDS 45.0%
AATGAAGTCAAAGGACGAGG+AGG + chr1.2:7039321-7039340 MS.gene016155:CDS 45.0%
GCAAATGAAGTCAAAGGACG+AGG + chr1.2:7039318-7039337 MS.gene016155:CDS 45.0%
GCAGTGACATTTGCGAGAAA+TGG + chr1.2:7039437-7039456 MS.gene016155:CDS 45.0%
ACACGGAGAGACTTTGTGTG+GGG + chr1.2:7039375-7039394 MS.gene016155:CDS 50.0%
CGTGAAGATTACTCCGGCAA+GGG + chr1.2:7039474-7039493 MS.gene016155:CDS 50.0%
GAACACGGAGAGACTTTGTG+TGG + chr1.2:7039373-7039392 MS.gene016155:CDS 50.0%
GCTATGCAGCTGATGAGTTC+TGG + chr1.2:7039410-7039429 MS.gene016155:CDS 50.0%
TTGATATGCTCAGCCCTTGC+CGG - chr1.2:7039490-7039509 None:intergenic 50.0%
AAGGACGAGGAGGTTGAGGA+TGG + chr1.2:7039331-7039350 MS.gene016155:CDS 55.0%
GAGACTTTGTGTGGGGCATG+TGG + chr1.2:7039382-7039401 MS.gene016155:CDS 55.0%
GAGGAGGTTGAGGATGGTGT+AGG + chr1.2:7039337-7039356 MS.gene016155:CDS 55.0%
GCGTGAAGATTACTCCGGCA+AGG + chr1.2:7039473-7039492 MS.gene016155:CDS 55.0%
GCTCTCTTGTTGCTGCAAGC+TGG - chr1.2:7039526-7039545 None:intergenic 55.0%
GGTGTGGACGAGGAAGAACA+CGG + chr1.2:7039358-7039377 MS.gene016155:CDS 55.0%
GGTTGAGGATGGTGTAGGTG+TGG + chr1.2:7039342-7039361 MS.gene016155:CDS 55.0%
GTCAAAGGACGAGGAGGTTG+AGG + chr1.2:7039327-7039346 MS.gene016155:CDS 55.0%
GGATGGTGTAGGTGTGGACG+AGG + chr1.2:7039348-7039367 MS.gene016155:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 7039310 7039555 7039310 ID=MS.gene016155
chr1.2 mRNA 7039310 7039555 7039310 ID=MS.gene016155.t1;Parent=MS.gene016155
chr1.2 exon 7039310 7039555 7039310 ID=MS.gene016155.t1.exon1;Parent=MS.gene016155.t1
chr1.2 CDS 7039310 7039555 7039310 ID=cds.MS.gene016155.t1;Parent=MS.gene016155.t1
Gene Sequence

>MS.gene016155

ATGGCAATGCAAATGAAGTCAAAGGACGAGGAGGTTGAGGATGGTGTAGGTGTGGACGAGGAAGAACACGGAGAGACTTTGTGTGGGGCATGTGGAGACAGCTATGCAGCTGATGAGTTCTGGATTTGCAGTGACATTTGCGAGAAATGGTTTCACGGAAAATGCGTGAAGATTACTCCGGCAAGGGCTGAGCATATCAAACAGTACAAATGTCCAGCTTGCAGCAACAAGAGAGCTCGCCCTTGA
Protein sequence

>MS.gene016155.t1

MAMQMKSKDEEVEDGVGVDEEEHGETLCGACGDSYAADEFWICSDICEKWFHGKCVKITPARAEHIKQYKCPACSNKRARP