AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016197

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016197.t1 MTR_1g016010 97.566 452 7 1 1 448 1 452 0.0 924
MS.gene016197.t1 MTR_2g099990 48.780 492 171 17 11 448 1 465 1.68e-132 390
MS.gene016197.t1 MTR_8g017210 47.129 505 187 14 1 433 1 497 4.97e-130 385
MS.gene016197.t1 MTR_2g099990 49.173 423 149 16 65 448 1 396 1.50e-112 337
MS.gene016197.t1 MTR_4g059870 73.711 194 50 1 16 208 12 205 4.32e-105 321
MS.gene016197.t1 MTR_2g093960 45.038 393 161 10 40 420 31 380 5.83e-102 310
MS.gene016197.t1 MTR_1g094115 76.216 185 41 3 38 222 45 226 7.88e-102 314
MS.gene016197.t1 MTR_1g112270 81.013 158 29 1 44 201 47 203 1.43e-97 304
MS.gene016197.t1 MTR_1g112270 75.740 169 29 2 44 201 47 214 6.21e-94 295
MS.gene016197.t1 MTR_2g090745 49.221 321 87 5 51 296 17 336 9.01e-93 287
MS.gene016197.t1 MTR_1g094115 69.189 185 37 4 38 222 45 209 1.45e-86 274
MS.gene016197.t1 MTR_2g017720 72.727 121 27 2 46 166 318 432 2.00e-52 182
MS.gene016197.t1 MTR_3g102000 48.193 166 68 5 68 229 36 187 2.94e-36 133
MS.gene016197.t1 MTR_5g030560 72.000 50 14 0 46 95 11 60 2.39e-21 88.2
MS.gene016197.t1 MTR_1g093095 34.161 161 91 6 47 194 91 249 1.89e-19 89.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016197.t1 AT1G55110 59.752 323 93 7 7 294 17 337 2.90e-120 358
MS.gene016197.t1 AT1G55110 59.752 323 93 7 7 294 17 337 2.90e-120 358
MS.gene016197.t1 AT1G55110 59.752 323 93 7 7 294 17 337 2.90e-120 358
MS.gene016197.t1 AT5G66730 75.000 196 41 3 10 203 4 193 5.27e-106 323
MS.gene016197.t1 AT3G50700 72.864 199 50 2 5 203 1 195 2.55e-105 320
MS.gene016197.t1 AT3G13810 69.444 216 46 2 8 203 17 232 1.77e-104 320
MS.gene016197.t1 AT3G13810 69.444 216 46 2 8 203 17 232 1.77e-104 320
MS.gene016197.t1 AT3G13810 69.444 216 46 2 8 203 12 227 2.55e-104 320
MS.gene016197.t1 AT3G45260 79.532 171 35 0 40 210 38 208 2.44e-103 315
MS.gene016197.t1 AT3G45260 79.532 171 35 0 40 210 38 208 2.44e-103 315
MS.gene016197.t1 AT4G02670 46.174 379 150 8 41 399 55 399 8.20e-103 312
MS.gene016197.t1 AT4G02670 46.174 379 150 8 41 399 53 397 8.77e-103 312
MS.gene016197.t1 AT1G03840 77.841 176 38 1 38 213 38 212 1.69e-102 315
MS.gene016197.t1 AT5G03150 76.966 178 38 1 40 214 52 229 1.71e-102 315
MS.gene016197.t1 AT3G13810 69.014 213 46 2 11 203 1 213 5.23e-102 313
MS.gene016197.t1 AT2G02080 78.916 166 34 1 38 203 51 215 1.04e-101 313
MS.gene016197.t1 AT2G02080 78.916 166 34 1 38 203 51 215 1.04e-101 313
MS.gene016197.t1 AT2G02080 78.916 166 34 1 38 203 51 215 1.04e-101 313
MS.gene016197.t1 AT2G02080 78.916 166 34 1 38 203 51 215 1.04e-101 313
MS.gene016197.t1 AT5G44160 75.000 188 46 1 24 211 20 206 2.26e-101 310
MS.gene016197.t1 AT1G14580 74.566 173 43 1 31 203 43 214 5.57e-101 310
MS.gene016197.t1 AT1G14580 74.566 173 43 1 31 203 43 214 5.57e-101 310
MS.gene016197.t1 AT1G14580 74.566 173 43 1 31 203 57 228 1.15e-100 309
MS.gene016197.t1 AT1G03840 76.705 176 38 2 38 213 38 210 7.63e-99 305
MS.gene016197.t1 AT2G02070 80.368 163 31 1 41 203 52 213 1.42e-98 308
MS.gene016197.t1 AT2G02070 80.368 163 31 1 41 203 52 213 1.42e-98 308
MS.gene016197.t1 AT5G60470 74.405 168 43 0 41 208 44 211 1.09e-96 298
MS.gene016197.t1 AT5G60470 74.405 168 43 0 41 208 40 207 1.27e-96 298
MS.gene016197.t1 AT3G13810 64.414 222 53 2 8 203 12 233 6.70e-95 295
MS.gene016197.t1 AT5G44160 79.141 163 33 1 49 211 6 167 3.22e-94 291
MS.gene016197.t1 AT3G13810 83.553 152 25 0 52 203 68 219 3.27e-94 293
MS.gene016197.t1 AT5G60470 73.885 157 41 0 52 208 46 202 5.04e-89 278
MS.gene016197.t1 AT2G02080 82.734 139 23 1 65 203 1 138 8.73e-88 275
MS.gene016197.t1 AT2G02080 82.734 139 23 1 65 203 1 138 8.73e-88 275
MS.gene016197.t1 AT1G68130 63.529 170 56 2 29 197 34 198 6.92e-75 241
MS.gene016197.t1 AT2G01940 62.722 169 61 2 28 195 32 199 2.85e-73 237
MS.gene016197.t1 AT1G25250 57.895 190 71 4 10 197 9 191 4.10e-72 232
MS.gene016197.t1 AT2G01940 61.765 170 62 3 28 195 32 200 1.03e-69 228
MS.gene016197.t1 AT1G25250 63.758 149 52 2 51 197 20 168 4.04e-68 221
MS.gene016197.t1 AT1G68130 64.286 112 39 1 87 197 1 112 1.46e-47 167
MS.gene016197.t1 AT1G25250 61.947 113 41 2 87 197 1 113 3.82e-45 159
MS.gene016197.t1 AT2G01940 62.727 110 40 1 87 195 1 110 1.27e-44 159
MS.gene016197.t1 AT1G34370 34.810 158 90 4 50 194 14 171 1.04e-22 98.2
MS.gene016197.t1 AT1G34370 34.810 158 90 4 50 194 224 381 3.75e-22 99.4
MS.gene016197.t1 AT1G34370 34.810 158 90 4 50 194 224 381 3.75e-22 99.4
MS.gene016197.t1 AT5G22890 31.395 172 103 4 36 194 185 354 4.41e-22 97.8
MS.gene016197.t1 AT1G13290 34.395 157 92 5 62 208 93 248 7.72e-20 90.1
MS.gene016197.t1 AT3G57670 32.749 171 96 6 62 215 209 377 3.57e-19 89.4
MS.gene016197.t1 AT3G20880 31.902 163 93 6 62 208 248 408 9.42e-19 88.6
MS.gene016197.t1 AT1G51220 33.803 142 83 5 62 194 170 309 1.22e-18 87.0
MS.gene016197.t1 AT1G34790 32.571 175 105 5 28 194 107 276 2.23e-17 82.8
MS.gene016197.t1 AT1G08290 34.507 142 83 4 62 194 172 312 2.32e-17 83.6

Find 84 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTAGTGCCACAAATTT+TGG 0.055414 1.2:+6460094 None:intergenic
TCTCATTGTTTGGCTTCTTA+TGG 0.135453 1.2:-6459209 MS.gene016197:CDS
GAGGAACAATATTCCCATTT+TGG 0.167286 1.2:-6459026 MS.gene016197:CDS
AACCATGGCTTTGATGAAAT+TGG 0.176098 1.2:+6459546 None:intergenic
AGTAACCATAATGGAATTTC+TGG 0.229667 1.2:+6459479 None:intergenic
CTCATTGTTTGGCTTCTTAT+GGG 0.236544 1.2:-6459208 MS.gene016197:CDS
TTTGAGTTGAAAGAATTATT+AGG 0.263924 1.2:+6459407 None:intergenic
AGGAAGATTTCTCTTCCTTT+TGG 0.267985 1.2:+6461022 None:intergenic
AGAGACTTGTTCAATATATC+TGG 0.300064 1.2:-6458921 MS.gene016197:CDS
GGAAACAAAGTTGTAGGGTT+AGG 0.304016 1.2:+6459428 None:intergenic
TGTTTGTTGCTAAGAGAGTT+TGG 0.304831 1.2:+6460408 None:intergenic
ATGCAAACTCACGTGCCTTC+AGG 0.323277 1.2:-6459275 MS.gene016197:CDS
TAAGACCAGAAATTCCATTA+TGG 0.323892 1.2:-6459484 MS.gene016197:CDS
GTTTGTTGCTAAGAGAGTTT+GGG 0.340724 1.2:+6460409 None:intergenic
TTCAATATATCTGGAATTGA+TGG 0.346876 1.2:-6458912 MS.gene016197:CDS
ACCATGGCTTTGATGAAATT+GGG 0.358760 1.2:+6459547 None:intergenic
ATGGCTTCACGTGAAGAAAT+AGG 0.371641 1.2:-6459245 MS.gene016197:CDS
TTATTAGGAAACAAAGTTGT+AGG 0.372201 1.2:+6459422 None:intergenic
AGGGCACTTGGTGACCTAAC+TGG 0.384172 1.2:-6460215 MS.gene016197:CDS
GAAACAAAGTTGTAGGGTTA+GGG 0.390096 1.2:+6459429 None:intergenic
AAAGCTAGCACTTGTGCCTC+AGG 0.393473 1.2:+6460437 None:intergenic
AGTGCTTCTTGATTCCAGTT+AGG 0.403331 1.2:+6460201 None:intergenic
GATAATGGTTCAAATGGGTT+GGG 0.421089 1.2:+6459456 None:intergenic
TGGGAGTGAGAAGAGAATAG+AGG 0.421836 1.2:+6459566 None:intergenic
TGATAATGGTTCAAATGGGT+TGG 0.442086 1.2:+6459455 None:intergenic
GGGAGTGAGAAGAGAATAGA+GGG 0.447356 1.2:+6459567 None:intergenic
GCACGTGAGTTTGCATTAAG+TGG 0.454018 1.2:+6459282 None:intergenic
TCTTGTCATCTCATCATCAT+TGG 0.457129 1.2:+6458974 None:intergenic
GCTTCTTCAGCAACCAAGAA+TGG 0.463212 1.2:-6461074 MS.gene016197:CDS
GTGTTCACCATGACCCTTCA+AGG 0.470912 1.2:-6460235 MS.gene016197:CDS
GAGAGATAATAATAACTTTG+AGG 0.471825 1.2:-6459045 MS.gene016197:CDS
CGTGAAGAAATAGGAACTAC+TGG 0.478841 1.2:-6459236 MS.gene016197:CDS
AAAAGGAAGAGAAATCTTCC+TGG 0.478919 1.2:-6461020 MS.gene016197:CDS
TGCATTAAGTGGGGTCTACA+AGG 0.485099 1.2:+6459293 None:intergenic
TATTAGGAAACAAAGTTGTA+GGG 0.486072 1.2:+6459423 None:intergenic
AAAGCTCATTCCAAAATTTG+TGG 0.492046 1.2:-6460104 MS.gene016197:CDS
AAAAGCAACATTATAAAGAA+TGG 0.495322 1.2:-6459002 MS.gene016197:CDS
AAACTCTCTTAGCAACAAAC+AGG 0.496554 1.2:-6460406 MS.gene016197:CDS
GTAAGCAAAATCAAACTCCT+TGG 0.497139 1.2:-6458872 MS.gene016197:CDS
AGATTCTTACCTTGAGAAGA+GGG 0.498855 1.2:+6460052 None:intergenic
GGAAGCACGGTGAGAAGAAG+TGG 0.500086 1.2:-6460172 MS.gene016197:CDS
CACGTGAGTTTGCATTAAGT+GGG 0.505578 1.2:+6459283 None:intergenic
ATCTAGAACCACAAGCCAAA+AGG 0.506626 1.2:-6461037 MS.gene016197:CDS
AACTCACGTGCCTTCAGGAA+TGG 0.523050 1.2:-6459270 MS.gene016197:CDS
TGGTGAACACATGTTGGCTC+TGG 0.526908 1.2:+6460248 None:intergenic
CATGACCCTTCAAGGGCACT+TGG 0.527782 1.2:-6460227 MS.gene016197:CDS
TGGGTTGGGAGTAACCATAA+TGG 0.528528 1.2:+6459470 None:intergenic
TCCCAATTTCATCAAAGCCA+TGG 0.529104 1.2:-6459548 MS.gene016197:CDS
AGGGATGATGAAAGGTTTGT+CGG 0.534689 1.2:-6461144 None:intergenic
GTTATGCAGTTCAGTCGGAT+TGG 0.535164 1.2:-6460127 MS.gene016197:CDS
CGTGCCTTCAGGAATGGCTA+TGG 0.546913 1.2:-6459264 MS.gene016197:CDS
TGCTTACCATGCAAACTCAA+AGG 0.555451 1.2:+6458888 None:intergenic
AGGTCACCAAGTGCCCTTGA+AGG 0.557241 1.2:+6460221 None:intergenic
TAAACGTTATGCAGTTCAGT+CGG 0.567043 1.2:-6460132 MS.gene016197:CDS
AAGATTCTTACCTTGAGAAG+AGG 0.572213 1.2:+6460051 None:intergenic
GATGGTCCTTTGAGTTTGCA+TGG 0.572532 1.2:-6458894 MS.gene016197:CDS
TGAGTGATAGGGATGATGAA+AGG 0.577909 1.2:-6461152 None:intergenic
CAAGTGCCCTTGAAGGGTCA+TGG 0.580343 1.2:+6460228 None:intergenic
TTACTGCTGCTATGAGCATG+AGG 0.586599 1.2:-6459085 MS.gene016197:CDS
GTGATAGTGGTTGAAAACCA+TGG 0.587939 1.2:+6459531 None:intergenic
TTTGTTGCTAAGAGAGTTTG+GGG 0.590009 1.2:+6460410 None:intergenic
AGAGAGGACATAATTTGCCA+TGG 0.596190 1.2:-6460325 MS.gene016197:CDS
ATTTGTGAGATTTGTAACAA+AGG 0.598660 1.2:-6460380 MS.gene016197:CDS
GGTCACCAAGTGCCCTTGAA+GGG 0.601914 1.2:+6460222 None:intergenic
AAGCTAGCACTTGTGCCTCA+GGG 0.605138 1.2:+6460438 None:intergenic
CTGTGATAAAACATCACAGA+AGG 0.605312 1.2:+6459616 None:intergenic
TGATGTGATGTATGGTAGTG+AGG 0.613764 1.2:-6459132 MS.gene016197:CDS
AATCTTCAGCTTCATAAGAG+AGG 0.614070 1.2:-6460341 MS.gene016197:CDS
TGTTCACCATGACCCTTCAA+GGG 0.614167 1.2:-6460234 MS.gene016197:CDS
GATGTGATGTATGGTAGTGA+GGG 0.620627 1.2:-6459131 MS.gene016197:CDS
AGCAACATTATAAAGAATGG+TGG 0.626398 1.2:-6458999 MS.gene016197:CDS
CAAAATCAAACTCCTTGGTG+AGG 0.632534 1.2:-6458867 None:intergenic
AGAAATCTTCCTGGCCACCC+AGG 0.633086 1.2:-6461011 MS.gene016197:intron
ACTGTGGAACCCTCTTCTCA+AGG 0.634375 1.2:-6460061 MS.gene016197:intron
GAAGCCATAGCCATTCCTGA+AGG 0.639906 1.2:+6459260 None:intergenic
AAAGAGTATAAGTGTGACTG+TGG 0.643514 1.2:-6460077 MS.gene016197:CDS
TACTGCTGCTATGAGCATGA+GGG 0.646189 1.2:-6459084 MS.gene016197:CDS
AAAGCAGTGGCAGACATGTG+AGG 0.650724 1.2:+6459365 None:intergenic
ACGTGAGTTTGCATTAAGTG+GGG 0.666291 1.2:+6459284 None:intergenic
GGAGAGTGCATCAATTTCAG+CGG 0.685024 1.2:-6459594 MS.gene016197:CDS
CCTGAATATCAGCCTCACCA+AGG 0.685282 1.2:+6458855 None:intergenic
GGGTCATGGTGAACACATGT+TGG 0.693614 1.2:+6460242 None:intergenic
ACTGCTGCTATGAGCATGAG+GGG 0.697079 1.2:-6459083 MS.gene016197:CDS
GATGATGAAAGGTTTGTCGG+TGG 0.706458 1.2:-6461141 MS.gene016197:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GAATCTTATTAATTTAAATT+TGG - chr1.2:6459974-6459993 MS.gene016197:intron 10.0%
!! CAAGAAAATAATATTACAAT+TGG + chr1.2:6459037-6459056 None:intergenic 15.0%
!! TATATACCAATTTATATTAC+TGG + chr1.2:6459076-6459095 None:intergenic 15.0%
!! TTCCCTAAAAAAAAATTAAT+AGG + chr1.2:6459467-6459486 None:intergenic 15.0%
!!! AGCCTATTAATTTTTTTTTA+GGG - chr1.2:6459462-6459481 MS.gene016197:CDS 15.0%
!!! TAGCCTATTAATTTTTTTTT+AGG - chr1.2:6459461-6459480 MS.gene016197:CDS 15.0%
!! AAAAGCAACATTATAAAGAA+TGG - chr1.2:6461007-6461026 MS.gene016197:intron 20.0%
!! ACATACAAATCAATTCAAAA+TGG - chr1.2:6460140-6460159 MS.gene016197:CDS 20.0%
!! TATAAATTGGTATATACTGA+AGG - chr1.2:6459080-6459099 MS.gene016197:CDS 20.0%
!!! GTTTTACAATTGAATATTTG+TGG - chr1.2:6459515-6459534 MS.gene016197:CDS 20.0%
!!! TATTATTTTCTTGTTCATGA+AGG - chr1.2:6459044-6459063 MS.gene016197:CDS 20.0%
!!! TTTGAGTTGAAAGAATTATT+AGG + chr1.2:6460605-6460624 None:intergenic 20.0%
! AAAAAAAGACAGGGTTTAAT+TGG - chr1.2:6459192-6459211 MS.gene016197:CDS 25.0%
! ACTGAAGGAGTTATATATAT+AGG - chr1.2:6459095-6459114 MS.gene016197:CDS 25.0%
! AGTAGTCCAGTAATATAAAT+TGG - chr1.2:6459067-6459086 MS.gene016197:CDS 25.0%
! ATTTGTGAGATTTGTAACAA+AGG - chr1.2:6459629-6459648 MS.gene016197:CDS 25.0%
! GAGAGATAATAATAACTTTG+AGG - chr1.2:6460964-6460983 MS.gene016197:intron 25.0%
! GATACAAACAAACTAAATCA+TGG - chr1.2:6460005-6460024 MS.gene016197:intron 25.0%
! GGCTGAAAATATTGTTAATT+TGG - chr1.2:6459536-6459555 MS.gene016197:CDS 25.0%
! TATTAGGAAACAAAGTTGTA+GGG + chr1.2:6460589-6460608 None:intergenic 25.0%
! TTACCTATCAAAAAAAGACA+GGG - chr1.2:6459183-6459202 MS.gene016197:CDS 25.0%
! TTATTAGGAAACAAAGTTGT+AGG + chr1.2:6460590-6460609 None:intergenic 25.0%
!! TTATATATATAGGAGGTGTA+GGG - chr1.2:6459105-6459124 MS.gene016197:CDS 25.0%
!! TTCAATATATCTGGAATTGA+TGG - chr1.2:6461097-6461116 MS.gene016197:CDS 25.0%
!!! AATGTTGCTTTTACCAAAAT+GGG + chr1.2:6460999-6461018 None:intergenic 25.0%
!!! AGTTTATTTTTGATTGCAGA+AGG - chr1.2:6460348-6460367 MS.gene016197:CDS 25.0%
!!! GTTTATTTTTGATTGCAGAA+GGG - chr1.2:6460349-6460368 MS.gene016197:CDS 25.0%
!!! GTTTTGATAATGGTTCAAAT+GGG + chr1.2:6460561-6460580 None:intergenic 25.0%
!!! TAATGTTGCTTTTACCAAAA+TGG + chr1.2:6461000-6461019 None:intergenic 25.0%
!!! TGTTAGTAGTTAGTTTGTTA+GGG + chr1.2:6459162-6459181 None:intergenic 25.0%
!!! TGTTCTTTTTTTAGTGATAG+TGG + chr1.2:6460494-6460513 None:intergenic 25.0%
AAAGCTCATTCCAAAATTTG+TGG - chr1.2:6459905-6459924 MS.gene016197:intron 30.0%
AGCAACATTATAAAGAATGG+TGG - chr1.2:6461010-6461029 MS.gene016197:intron 30.0%
CAATATATCACCTAAATCCA+TGG + chr1.2:6459256-6459275 None:intergenic 30.0%
CTTACCTATCAAAAAAAGAC+AGG - chr1.2:6459182-6459201 MS.gene016197:CDS 30.0%
GAAGGAGTTATATATATAGG+AGG - chr1.2:6459098-6459117 MS.gene016197:CDS 30.0%
TAAGACCAGAAATTCCATTA+TGG - chr1.2:6460525-6460544 MS.gene016197:intron 30.0%
TGGATTTAGGTGATATATTG+TGG - chr1.2:6459256-6459275 MS.gene016197:CDS 30.0%
TTGTCATAACTCATATCTCT+TGG + chr1.2:6459233-6459252 None:intergenic 30.0%
! AGAGACTTGTTCAATATATC+TGG - chr1.2:6461088-6461107 MS.gene016197:CDS 30.0%
! AGTAACCATAATGGAATTTC+TGG + chr1.2:6460533-6460552 None:intergenic 30.0%
! ATTGGTGTGATTTTATTACC+TGG + chr1.2:6459019-6459038 None:intergenic 30.0%
! GTTATATATATAGGAGGTGT+AGG - chr1.2:6459104-6459123 MS.gene016197:CDS 30.0%
! TTGGTGTGATTTTATTACCT+GGG + chr1.2:6459018-6459037 None:intergenic 30.0%
!! GTGTTAGTAGTTAGTTTGTT+AGG + chr1.2:6459163-6459182 None:intergenic 30.0%
!! TTTCTTTTCTTTAGCTTCCA+TGG + chr1.2:6459704-6459723 None:intergenic 30.0%
!!! AAACCCTGTCTTTTTTTGAT+AGG + chr1.2:6459189-6459208 None:intergenic 30.0%
!!! ACAAGAGATTTTTTGAGTCT+TGG - chr1.2:6461052-6461071 MS.gene016197:CDS 30.0%
!!! GGTTTTGATAATGGTTCAAA+TGG + chr1.2:6460562-6460581 None:intergenic 30.0%
!!! TACTGGTTTTTCTCATTGTT+TGG - chr1.2:6460790-6460809 MS.gene016197:intron 30.0%
AAAAGGAAGAGAAATCTTCC+TGG - chr1.2:6458989-6459008 MS.gene016197:CDS 35.0%
AAACTCTCTTAGCAACAAAC+AGG - chr1.2:6459603-6459622 MS.gene016197:CDS 35.0%
AAGATTCTTACCTTGAGAAG+AGG + chr1.2:6459961-6459980 None:intergenic 35.0%
AATCTTCAGCTTCATAAGAG+AGG - chr1.2:6459668-6459687 MS.gene016197:intron 35.0%
ACAAAGAAAAGTAGTAGCCA+AGG + chr1.2:6459405-6459424 None:intergenic 35.0%
AGATTCTTACCTTGAGAAGA+GGG + chr1.2:6459960-6459979 None:intergenic 35.0%
CAAAGAAAAGTAGTAGCCAA+GGG + chr1.2:6459404-6459423 None:intergenic 35.0%
CTGTGATAAAACATCACAGA+AGG + chr1.2:6460396-6460415 None:intergenic 35.0%
GAAACAAAGTTGTAGGGTTA+GGG + chr1.2:6460583-6460602 None:intergenic 35.0%
GATATGAGTTATGACAACCA+TGG - chr1.2:6459236-6459255 MS.gene016197:CDS 35.0%
GTAAGCAAAATCAAACTCCT+TGG - chr1.2:6461137-6461156 MS.gene016197:CDS 35.0%
GTTATGACAACCATGGATTT+AGG - chr1.2:6459243-6459262 MS.gene016197:CDS 35.0%
TAAACGTTATGCAGTTCAGT+CGG - chr1.2:6459877-6459896 MS.gene016197:intron 35.0%
TCTTGTCATCTCATCATCAT+TGG + chr1.2:6461038-6461057 None:intergenic 35.0%
TTTGTTGCTAAGAGAGTTTG+GGG + chr1.2:6459602-6459621 None:intergenic 35.0%
! AAAGAGTATAAGTGTGACTG+TGG - chr1.2:6459932-6459951 MS.gene016197:intron 35.0%
! AAGAGAGAGAGTACGTAAAA+AGG - chr1.2:6460068-6460087 MS.gene016197:CDS 35.0%
! AGGAAGATTTCTCTTCCTTT+TGG + chr1.2:6458990-6459009 None:intergenic 35.0%
! CTCATTGTTTGGCTTCTTAT+GGG - chr1.2:6460801-6460820 MS.gene016197:intron 35.0%
! CTCTCTCTCTTATCTCTTTT+TGG + chr1.2:6460059-6460078 None:intergenic 35.0%
! CTCTTTAGTGCCACAAATTT+TGG + chr1.2:6459918-6459937 None:intergenic 35.0%
! CTTCTGTGATGTTTTATCAC+AGG - chr1.2:6460394-6460413 MS.gene016197:CDS 35.0%
! CTTTTGCATGATGTGATGTA+TGG - chr1.2:6460869-6460888 MS.gene016197:intron 35.0%
! GAGGAACAATATTCCCATTT+TGG - chr1.2:6460983-6461002 MS.gene016197:intron 35.0%
! GATAATGGTTCAAATGGGTT+GGG + chr1.2:6460556-6460575 None:intergenic 35.0%
! GTTTGTTGCTAAGAGAGTTT+GGG + chr1.2:6459603-6459622 None:intergenic 35.0%
! TCTCATTGTTTGGCTTCTTA+TGG - chr1.2:6460800-6460819 MS.gene016197:intron 35.0%
! TGTTGAAGCTTTTCCATTCT+TGG + chr1.2:6458951-6458970 None:intergenic 35.0%
! TGTTTGTTGCTAAGAGAGTT+TGG + chr1.2:6459604-6459623 None:intergenic 35.0%
!! AACCATGGCTTTGATGAAAT+TGG + chr1.2:6460466-6460485 None:intergenic 35.0%
!! ACCATGGCTTTGATGAAATT+GGG + chr1.2:6460465-6460484 None:intergenic 35.0%
!! CTGCAAAAAGCATCACAAAT+AGG - chr1.2:6460668-6460687 MS.gene016197:intron 35.0%
!! TAGGGTTAGGGTTTTGATAA+TGG + chr1.2:6460571-6460590 None:intergenic 35.0%
!! TAGTTAGTTTGTTAGGGAAG+TGG + chr1.2:6459156-6459175 None:intergenic 35.0%
!! TGATAATGGTTCAAATGGGT+TGG + chr1.2:6460557-6460576 None:intergenic 35.0%
!!! GAATCAAGAAGCACTTTTTC+AGG - chr1.2:6459816-6459835 MS.gene016197:intron 35.0%
ACACTGATGCATAGTCACAT+AGG - chr1.2:6459361-6459380 MS.gene016197:CDS 40.0%
ACGTGAGTTTGCATTAAGTG+GGG + chr1.2:6460728-6460747 None:intergenic 40.0%
AGTGCTTCTTGATTCCAGTT+AGG + chr1.2:6459811-6459830 None:intergenic 40.0%
ATGGCTTCACGTGAAGAAAT+AGG - chr1.2:6460764-6460783 MS.gene016197:intron 40.0%
CACGTGAGTTTGCATTAAGT+GGG + chr1.2:6460729-6460748 None:intergenic 40.0%
CGTGAAGAAATAGGAACTAC+TGG - chr1.2:6460773-6460792 MS.gene016197:intron 40.0%
GGAAACAAAGTTGTAGGGTT+AGG + chr1.2:6460584-6460603 None:intergenic 40.0%
TCCCAATTTCATCAAAGCCA+TGG - chr1.2:6460461-6460480 MS.gene016197:intron 40.0%
TCTGATGATCGTGTTAGAGT+CGG + chr1.2:6459298-6459317 None:intergenic 40.0%
TGCTTACCATGCAAACTCAA+AGG + chr1.2:6461124-6461143 None:intergenic 40.0%
! AGAGAGGACATAATTTGCCA+TGG - chr1.2:6459684-6459703 MS.gene016197:intron 40.0%
! ATCTAGAACCACAAGCCAAA+AGG - chr1.2:6458972-6458991 MS.gene016197:CDS 40.0%
! GATGTGATGTATGGTAGTGA+GGG - chr1.2:6460878-6460897 MS.gene016197:intron 40.0%
! GTGTGATTTTATTACCTGGG+TGG + chr1.2:6459015-6459034 None:intergenic 40.0%
! TGATGTGATGTATGGTAGTG+AGG - chr1.2:6460877-6460896 MS.gene016197:intron 40.0%
!! GTGATAGTGGTTGAAAACCA+TGG + chr1.2:6460481-6460500 None:intergenic 40.0%
!!! AAGCACTTTTTCAGGAAGCA+CGG - chr1.2:6459824-6459843 MS.gene016197:intron 40.0%
!!! TTTCTCTTCCTTTTGGCTTG+TGG + chr1.2:6458983-6459002 None:intergenic 40.0%
GCACGTGAGTTTGCATTAAG+TGG + chr1.2:6460730-6460749 None:intergenic 45.0%
GCTTCTTCAGCAACCAAGAA+TGG - chr1.2:6458935-6458954 MS.gene016197:CDS 45.0%
GGGAGTGAGAAGAGAATAGA+GGG + chr1.2:6460445-6460464 None:intergenic 45.0%
GTGTTTGACGTTTACTCTGC+AGG - chr1.2:6460197-6460216 MS.gene016197:CDS 45.0%
GTTATGCAGTTCAGTCGGAT+TGG - chr1.2:6459882-6459901 MS.gene016197:intron 45.0%
TACTGCTGCTATGAGCATGA+GGG - chr1.2:6460925-6460944 MS.gene016197:intron 45.0%
TGCATTAAGTGGGGTCTACA+AGG + chr1.2:6460719-6460738 None:intergenic 45.0%
TGGGAGTGAGAAGAGAATAG+AGG + chr1.2:6460446-6460465 None:intergenic 45.0%
TGTTCACCATGACCCTTCAA+GGG - chr1.2:6459775-6459794 MS.gene016197:intron 45.0%
TTACTGCTGCTATGAGCATG+AGG - chr1.2:6460924-6460943 MS.gene016197:intron 45.0%
! ATTTTATTACCTGGGTGGCC+AGG + chr1.2:6459010-6459029 None:intergenic 45.0%
! GATGGTCCTTTGAGTTTGCA+TGG - chr1.2:6461115-6461134 MS.gene016197:CDS 45.0%
! GGAGAGTGCATCAATTTCAG+CGG - chr1.2:6460415-6460434 MS.gene016197:CDS 45.0%
! TGGGTTGGGAGTAACCATAA+TGG + chr1.2:6460542-6460561 None:intergenic 45.0%
!! TGCTTTTTGCAGCAAAGCAG+TGG + chr1.2:6460660-6460679 None:intergenic 45.0%
!!! TGGTTTTGTTTCAGACCCTG+AGG - chr1.2:6459556-6459575 MS.gene016197:CDS 45.0%
!! ATATAACATTAATATATAAT+AGG + chr1.2:6459494-6459513 None:intergenic 5.0%
AAAGCAGTGGCAGACATGTG+AGG + chr1.2:6460647-6460666 None:intergenic 50.0%
AAAGCTAGCACTTGTGCCTC+AGG + chr1.2:6459575-6459594 None:intergenic 50.0%
AACTCACGTGCCTTCAGGAA+TGG - chr1.2:6460739-6460758 MS.gene016197:intron 50.0%
AAGCTAGCACTTGTGCCTCA+GGG + chr1.2:6459574-6459593 None:intergenic 50.0%
ACTGCTGCTATGAGCATGAG+GGG - chr1.2:6460926-6460945 MS.gene016197:intron 50.0%
ACTGTGGAACCCTCTTCTCA+AGG - chr1.2:6459948-6459967 MS.gene016197:intron 50.0%
ATGCAAACTCACGTGCCTTC+AGG - chr1.2:6460734-6460753 MS.gene016197:intron 50.0%
GAAGCCATAGCCATTCCTGA+AGG + chr1.2:6460752-6460771 None:intergenic 50.0%
GGGTCATGGTGAACACATGT+TGG + chr1.2:6459770-6459789 None:intergenic 50.0%
GTCACATAGGTAGCACCATG+AGG - chr1.2:6459374-6459393 MS.gene016197:CDS 50.0%
GTGTTCACCATGACCCTTCA+AGG - chr1.2:6459774-6459793 MS.gene016197:intron 50.0%
!! TGGTGAACACATGTTGGCTC+TGG + chr1.2:6459764-6459783 None:intergenic 50.0%
AGAAATCTTCCTGGCCACCC+AGG - chr1.2:6458998-6459017 MS.gene016197:CDS 55.0%
AGGTCACCAAGTGCCCTTGA+AGG + chr1.2:6459791-6459810 None:intergenic 55.0%
CAAGTGCCCTTGAAGGGTCA+TGG + chr1.2:6459784-6459803 None:intergenic 55.0%
CGTGCCTTCAGGAATGGCTA+TGG - chr1.2:6460745-6460764 MS.gene016197:intron 55.0%
GGTCACCAAGTGCCCTTGAA+GGG + chr1.2:6459790-6459809 None:intergenic 55.0%
! AGGGCACTTGGTGACCTAAC+TGG - chr1.2:6459794-6459813 MS.gene016197:intron 55.0%
! GGAAGCACGGTGAGAAGAAG+TGG - chr1.2:6459837-6459856 MS.gene016197:intron 55.0%
!! CATGACCCTTCAAGGGCACT+TGG - chr1.2:6459782-6459801 MS.gene016197:intron 55.0%
! AGCACCATGAGGTGCTCCCT+TGG - chr1.2:6459385-6459404 MS.gene016197:CDS 60.0%
!! GTAGCCAAGGGAGCACCTCA+TGG + chr1.2:6459392-6459411 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 6458869 6461162 6458869 ID=MS.gene016197
chr1.2 mRNA 6458869 6461162 6458869 ID=MS.gene016197.t1;Parent=MS.gene016197
chr1.2 exon 6461012 6461162 6461012 ID=MS.gene016197.t1.exon1;Parent=MS.gene016197.t1
chr1.2 CDS 6461012 6461162 6461012 ID=cds.MS.gene016197.t1;Parent=MS.gene016197.t1
chr1.2 exon 6460062 6460461 6460062 ID=MS.gene016197.t1.exon2;Parent=MS.gene016197.t1
chr1.2 CDS 6460062 6460461 6460062 ID=cds.MS.gene016197.t1;Parent=MS.gene016197.t1
chr1.2 exon 6458869 6459664 6458869 ID=MS.gene016197.t1.exon3;Parent=MS.gene016197.t1
chr1.2 CDS 6458869 6459664 6458869 ID=cds.MS.gene016197.t1;Parent=MS.gene016197.t1
Gene Sequence

>MS.gene016197

ATGATGAAAGGTTTGTCGGTGGATGAAAATATGTCAAATTTGACATCTGCTTCAAGTGAAATAAGTGCTTCTTCAGCAACCAAGAATGGAAAAGCTTCAACAAATCTAGAACCACAAGCCAAAAGGAAGAGAAATCTTCCTGGCCACCCAGGTAATAAAATCACACCAATTGTAATATTATTTTCTTGTTCATGAAGGAGTAGTCCAGTAATATAAATTGGTATATACTGAAGGAGTTATATATATAGGAGGTGTAGGGTTTGATATTTGTTACTCAACATTTTCCACTTCCCTAACAAACTAACTACTAACACTTACCTATCAAAAAAAGACAGGGTTTAATTGGTGCAACAATCTATCACCAAGAGATATGAGTTATGACAACCATGGATTTAGGTGATATATTGTGGTATGAGATATGAAGCACCGACTCTAACACGATCATCAGACATGACACTAGCATGTTGACACTTTTAACTTCAAAAACATAGCACACTGATGCATAGTCACATAGGTAGCACCATGAGGTGCTCCCTTGGCTACTACTTTTCTTTGTAAAAGAAAAATTGAAAAAAAATGTCGTCTAAATTATTAGCCTATTAATTTTTTTTTAGGGAATTAGCCTATTATATATTAATGTTATATTGTTTTACAATTGAATATTTGTGGCTGAAAATATTGTTAATTTGGTTTTGTTTCAGACCCTGAGGCACAAGTGCTAGCTTTGAGTCCCCAAACTCTCTTAGCAACAAACAGGTTCATTTGTGAGATTTGTAACAAAGGTTTTCAAAGAGATCAAAATCTTCAGCTTCATAAGAGAGGACATAATTTGCCATGGAAGCTAAAGAAAAGAAACGTCAATGAAATCATAAGAAAGAAAGTATATGTTTGTCCAGAGCCAACATGTGTTCACCATGACCCTTCAAGGGCACTTGGTGACCTAACTGGAATCAAGAAGCACTTTTTCAGGAAGCACGGTGAGAAGAAGTGGAAGTGTGAGAAATGTTCTAAACGTTATGCAGTTCAGTCGGATTGGAAAGCTCATTCCAAAATTTGTGGCACTAAAGAGTATAAGTGTGACTGTGGAACCCTCTTCTCAAGGTAAGAATCTTATTAATTTAAATTTGGTCATTATAGATACAAACAAACTAAATCATGGTACGTTCAATAATAATGAGTCAAAAAATCCAAAAAGAGATAAGAGAGAGAGTACGTAAAAAGGTAAAAAGTGCTTAAACAGTATTTTATTAGTTCATAGAAAATGTTTTGTGACATACAAATCAATTCAAAATGGTCAGATAATTGTTTGAGAGAACTTGAATTCAAATGTGTTTGACGTTTACTCTGCAGGTAACAAACTATAATGCATGTATTTTTTCAATATATATTTGAGATTAATTAAATAATGATATGATGTATATACTTTAAATTTAAGTTACTCTCATGACAAAATTATATTTAATTCATCAAATAATTTTGAGTTTATTTTTGATTGCAGAAGGGACAGTTTTATCACTCACAGAGCCTTCTGTGATGTTTTATCACAGGAGAGTGCATCAATTTCAGCGGTGAACCCTCTATTCTCTTCTCACTCCCAATTTCATCAAAGCCATGGTTTTCAACCACTATCACTAAAAAAAGAACAAGACTTCAATTTAAGACCAGAAATTCCATTATGGTTACTCCCAACCCATTTGAACCATTATCAAAACCCTAACCCTACAACTTTGTTTCCTAATAATTCTTTCAACTCAAACAATATGACTACTGCTTCTCCTCACATGTCTGCCACTGCTTTGCTGCAAAAAGCATCACAAATAGGTGTAACGACAGTGAGTAAAACAGAACCTTGTAGACCCCACTTAATGCAAACTCACGTGCCTTCAGGAATGGCTATGGCTTCACGTGAAGAAATAGGAACTACTGGTTTTTCTCATTGTTTGGCTTCTTATGGGAATAAAGCTGCAATAACTTCTGATTGCTTTGAAGAATCTTCTTCTCTTTTGCATGATGTGATGTATGGTAGTGAGGGCTCACATTCACAATTTGAAGCTGTTACTGCTGCTATGAGCATGAGGGGAATGTTTGATACACAGAGAGATAATAATAACTTTGAGGAACAATATTCCCATTTTGGTAAAAGCAACATTATAAAGAATGGTGGTGCCAATGATGATGAGATGACAAGAGATTTTTTGAGTCTTGGTGCATTTTCTCAAAGAGACTTGTTCAATATATCTGGAATTGATGGTCCTTTGAGTTTGCATGGTAAGCAAAATCAAACTCCTTGGTGA
Protein sequence

>MS.gene016197.t1

MMKGLSVDENMSNLTSASSEISASSATKNGKASTNLEPQAKRKRNLPGHPDPEAQVLALSPQTLLATNRFICEICNKGFQRDQNLQLHKRGHNLPWKLKKRNVNEIIRKKVYVCPEPTCVHHDPSRALGDLTGIKKHFFRKHGEKKWKCEKCSKRYAVQSDWKAHSKICGTKEYKCDCGTLFSRRDSFITHRAFCDVLSQESASISAVNPLFSSHSQFHQSHGFQPLSLKKEQDFNLRPEIPLWLLPTHLNHYQNPNPTTLFPNNSFNSNNMTTASPHMSATALLQKASQIGVTTVSKTEPCRPHLMQTHVPSGMAMASREEIGTTGFSHCLASYGNKAAITSDCFEESSSLLHDVMYGSEGSHSQFEAVTAAMSMRGMFDTQRDNNNFEEQYSHFGKSNIIKNGGANDDEMTRDFLSLGAFSQRDLFNISGIDGPLSLHGKQNQTPW