Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016231.t1 | RHN46778.1 | 92.1 | 165 | 13 | 0 | 146 | 310 | 80 | 244 | 3.00E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016231.t1 | O22131 | 37.9 | 174 | 95 | 4 | 146 | 307 | 90 | 262 | 1.4e-13 | 78.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016231.t1 | G7L095 | 92.1 | 165 | 13 | 0 | 146 | 310 | 73 | 237 | 2.2e-68 | 268.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene016231.t1 | TF | LOB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016231.t1 | MTR_7g074990 | 96.970 | 165 | 5 | 0 | 146 | 310 | 73 | 237 | 1.24e-112 | 326 |
| MS.gene016231.t1 | MTR_8g036085 | 47.531 | 162 | 80 | 4 | 146 | 307 | 76 | 232 | 2.09e-35 | 128 |
| MS.gene016231.t1 | MTR_8g036105 | 73.469 | 49 | 13 | 0 | 144 | 192 | 69 | 117 | 3.01e-18 | 82.0 |
| MS.gene016231.t1 | MTR_7g075230 | 62.000 | 50 | 19 | 0 | 146 | 195 | 67 | 116 | 1.81e-15 | 73.9 |
| MS.gene016231.t1 | MTR_1g070205 | 59.322 | 59 | 18 | 1 | 145 | 197 | 64 | 122 | 4.69e-15 | 73.6 |
| MS.gene016231.t1 | MTR_5g083960 | 45.349 | 86 | 41 | 1 | 145 | 230 | 90 | 169 | 2.58e-14 | 70.9 |
| MS.gene016231.t1 | MTR_7g096610 | 66.667 | 45 | 15 | 0 | 147 | 191 | 62 | 106 | 4.79e-14 | 70.5 |
| MS.gene016231.t1 | MTR_6g027710 | 49.296 | 71 | 30 | 1 | 144 | 208 | 91 | 161 | 1.46e-13 | 69.3 |
| MS.gene016231.t1 | MTR_3g094690 | 61.404 | 57 | 22 | 0 | 147 | 203 | 100 | 156 | 1.60e-13 | 69.3 |
| MS.gene016231.t1 | MTR_3g094690 | 61.404 | 57 | 22 | 0 | 147 | 203 | 99 | 155 | 1.62e-13 | 69.3 |
| MS.gene016231.t1 | MTR_7g096530 | 56.863 | 51 | 22 | 0 | 144 | 194 | 65 | 115 | 1.06e-12 | 66.6 |
| MS.gene016231.t1 | MTR_1g070220 | 68.293 | 41 | 13 | 0 | 147 | 187 | 69 | 109 | 1.49e-11 | 63.2 |
| MS.gene016231.t1 | MTR_5g083230 | 40.789 | 76 | 45 | 0 | 146 | 221 | 58 | 133 | 1.50e-11 | 62.4 |
| MS.gene016231.t1 | MTR_5g080470 | 59.574 | 47 | 19 | 0 | 146 | 192 | 60 | 106 | 1.75e-11 | 62.8 |
| MS.gene016231.t1 | MTR_4g105170 | 46.377 | 69 | 36 | 1 | 146 | 214 | 63 | 130 | 2.15e-11 | 62.4 |
| MS.gene016231.t1 | MTR_3g073690 | 49.231 | 65 | 32 | 1 | 144 | 208 | 59 | 122 | 2.77e-11 | 62.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016231.t1 | AT2G45420 | 44.231 | 156 | 73 | 4 | 146 | 288 | 90 | 244 | 6.66e-27 | 106 |
| MS.gene016231.t1 | AT4G00220 | 40.854 | 164 | 81 | 6 | 146 | 303 | 70 | 223 | 6.34e-24 | 97.8 |
| MS.gene016231.t1 | AT4G00210 | 64.179 | 67 | 19 | 1 | 144 | 210 | 62 | 123 | 2.48e-20 | 87.8 |
| MS.gene016231.t1 | AT2G45410 | 51.429 | 70 | 31 | 1 | 146 | 215 | 69 | 135 | 2.59e-17 | 79.0 |
| MS.gene016231.t1 | AT3G03760 | 67.925 | 53 | 16 | 1 | 145 | 196 | 103 | 155 | 2.81e-14 | 72.0 |
| MS.gene016231.t1 | AT3G58190 | 33.333 | 141 | 82 | 4 | 145 | 284 | 63 | 192 | 6.13e-14 | 70.1 |
| MS.gene016231.t1 | AT5G06080 | 43.038 | 79 | 38 | 1 | 146 | 217 | 60 | 138 | 2.16e-12 | 65.1 |
| MS.gene016231.t1 | AT2G30340 | 60.000 | 50 | 20 | 0 | 145 | 194 | 104 | 153 | 2.06e-11 | 63.5 |
| MS.gene016231.t1 | AT2G30340 | 60.000 | 50 | 20 | 0 | 145 | 194 | 103 | 152 | 2.29e-11 | 63.5 |
| MS.gene016231.t1 | AT2G42430 | 54.167 | 48 | 22 | 0 | 147 | 194 | 69 | 116 | 9.41e-11 | 61.6 |
| MS.gene016231.t1 | AT5G63090 | 50.000 | 58 | 29 | 0 | 146 | 203 | 63 | 120 | 9.48e-11 | 60.5 |
| MS.gene016231.t1 | AT5G63090 | 50.000 | 58 | 29 | 0 | 146 | 203 | 63 | 120 | 9.48e-11 | 60.5 |
| MS.gene016231.t1 | AT5G63090 | 50.000 | 58 | 29 | 0 | 146 | 203 | 63 | 120 | 9.48e-11 | 60.5 |
| MS.gene016231.t1 | AT5G63090 | 50.000 | 58 | 29 | 0 | 146 | 203 | 63 | 120 | 9.48e-11 | 60.5 |
Find 78 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCATTGGTTCTGATTTC+TGG | -0.030317 | 7.4:+30261556 | None:intergenic |
| GACTGATCATTTAGAAAATT+TGG | 0.116735 | 7.4:+30255075 | None:intergenic |
| GATTGGCAGGCTTAACTTGC+TGG | 0.274437 | 7.4:-30268462 | MS.gene016231:CDS |
| TTTATGAGAAACATCTGATT+TGG | 0.276769 | 7.4:+30271677 | None:intergenic |
| CATGGCGGGGCTGGCTGAAT+TGG | 0.284236 | 7.4:+30268488 | None:intergenic |
| CCCCGCCATGTTTGATTGGC+AGG | 0.289247 | 7.4:-30268475 | MS.gene016231:CDS |
| GACCTTACTCATAGACAAAT+TGG | 0.304306 | 7.4:-30255145 | MS.gene016231:CDS |
| GTGGTGGTAGCGGTGCTTGT+TGG | 0.310892 | 7.4:+30255392 | None:intergenic |
| TTGAATTAATGTGGCTTTGT+AGG | 0.334987 | 7.4:+30255425 | None:intergenic |
| GACCAAACTTTACATGTAAA+GGG | 0.339182 | 7.4:+30271770 | None:intergenic |
| CCAATCAAACATGGCGGGGC+TGG | 0.343042 | 7.4:+30268479 | None:intergenic |
| CAAAGCCACATTAATTCAAC+TGG | 0.351989 | 7.4:-30255421 | MS.gene016231:CDS |
| GCCCCTTTACATGTAAAGTT+TGG | 0.358182 | 7.4:-30271772 | MS.gene016231:CDS |
| TCCCAGCGGTGGTTGATGTT+GGG | 0.361607 | 7.4:+30255185 | None:intergenic |
| CCAGCCCCGCCATGTTTGAT+TGG | 0.379804 | 7.4:-30268479 | MS.gene016231:CDS |
| TGGTGGTAGCGGTGCTTGTT+GGG | 0.396654 | 7.4:+30255393 | None:intergenic |
| TGACCAAACTTTACATGTAA+AGG | 0.403569 | 7.4:+30271769 | None:intergenic |
| TCATTTGAGATTCAATCAAT+TGG | 0.412405 | 7.4:-30271749 | MS.gene016231:CDS |
| CATCGTTGCCCTTCAGCAAC+AGG | 0.412822 | 7.4:-30258342 | MS.gene016231:intron |
| GGCATGGTTGTGGCGGTGGT+TGG | 0.418748 | 7.4:+30255322 | None:intergenic |
| GACCGGCATGGTTGTGGCGG+TGG | 0.423968 | 7.4:+30255318 | None:intergenic |
| ATGGTTGTGGCGGTGGTTGG+TGG | 0.442641 | 7.4:+30255325 | None:intergenic |
| TCCAACAGTCTTCTCCTTTG+CGG | 0.443005 | 7.4:-30255353 | MS.gene016231:CDS |
| TGATTTGGAAATGATTGCAT+TGG | 0.456982 | 7.4:+30271692 | None:intergenic |
| AGGTTGAGAGATCCTATCTA+TGG | 0.467327 | 7.4:-30258377 | MS.gene016231:CDS |
| ATAGGTGACCGGCATGGTTG+TGG | 0.472293 | 7.4:+30255312 | None:intergenic |
| GCCGCAGTCGCCTGTGGTGG+TGG | 0.480245 | 7.4:+30255376 | None:intergenic |
| GCCTGCCAATCAAACATGGC+GGG | 0.483659 | 7.4:+30268474 | None:intergenic |
| TGACCAACACAACCATAGAT+AGG | 0.484861 | 7.4:+30258365 | None:intergenic |
| GTATTGACGTGGATCAATAC+TGG | 0.487488 | 7.4:+30255228 | None:intergenic |
| ATCCCAGCGGTGGTTGATGT+TGG | 0.489865 | 7.4:+30255184 | None:intergenic |
| AGCACCGCTACCACCACCAC+AGG | 0.491142 | 7.4:-30255386 | MS.gene016231:CDS |
| ATTAATGTGGCTTTGTAGGT+AGG | 0.492254 | 7.4:+30255429 | None:intergenic |
| AGCCAATTTGTCTATGAGTA+AGG | 0.502031 | 7.4:+30255143 | None:intergenic |
| AATAAGTTACCTGTTGCTGA+AGG | 0.502270 | 7.4:+30258333 | None:intergenic |
| GCCCCAACATCAACCACCGC+TGG | 0.504868 | 7.4:-30255187 | MS.gene016231:CDS |
| TTTGCTATGAAGCTCAATCA+AGG | 0.514259 | 7.4:-30258397 | MS.gene016231:CDS |
| CGAAGCAGATGATGAGGCAT+TGG | 0.523565 | 7.4:+30255102 | None:intergenic |
| ATACTAGTAACAGCGGTAGC+AGG | 0.527262 | 7.4:+30268413 | None:intergenic |
| CCAATGGAAGGAAATGCAAA+AGG | 0.527300 | 7.4:-30261543 | MS.gene016231:CDS |
| CCCAGCGGTGGTTGATGTTG+GGG | 0.527927 | 7.4:+30255186 | None:intergenic |
| TTGTGGCGGTGGTTGGTGGT+AGG | 0.533093 | 7.4:+30255329 | None:intergenic |
| GTCGCCTGTGGTGGTGGTAG+CGG | 0.534456 | 7.4:+30255382 | None:intergenic |
| GCAAAAGGCAAATGTACTAC+AGG | 0.537299 | 7.4:-30261528 | MS.gene016231:intron |
| TCCGCAAAGGAGAAGACTGT+TGG | 0.538182 | 7.4:+30255352 | None:intergenic |
| TTGGCATTCCAGCTAGTAGG+AGG | 0.542403 | 7.4:-30258439 | MS.gene016231:intron |
| TTCTTGGCATTCCAGCTAGT+AGG | 0.545620 | 7.4:-30258442 | MS.gene016231:intron |
| TGTTGTTGCATGGACAATGA+AGG | 0.552755 | 7.4:+30255256 | None:intergenic |
| AGTCCAGAAATCAGAACCAA+TGG | 0.554200 | 7.4:-30261559 | MS.gene016231:CDS |
| CCCCAACATCAACCACCGCT+GGG | 0.555064 | 7.4:-30255186 | MS.gene016231:CDS |
| GATCCTATCTATGGTTGTGT+TGG | 0.563587 | 7.4:-30258368 | MS.gene016231:CDS |
| AACCACCGCCACAACCATGC+CGG | 0.571081 | 7.4:-30255320 | MS.gene016231:CDS |
| TTAGCCCAGTTGAATTAATG+TGG | 0.586405 | 7.4:+30255416 | None:intergenic |
| ATAAGTTACCTGTTGCTGAA+GGG | 0.587056 | 7.4:+30258334 | None:intergenic |
| AAAGGTGATATTGAAAGTCG+TGG | 0.590876 | 7.4:-30261609 | MS.gene016231:CDS |
| ACTGGTCCTCTGTTGTTGCA+TGG | 0.593499 | 7.4:+30255246 | None:intergenic |
| CCTGCCAATCAAACATGGCG+GGG | 0.603264 | 7.4:+30268475 | None:intergenic |
| GTTGGAGCCGCAGTCGCCTG+TGG | 0.606034 | 7.4:+30255370 | None:intergenic |
| GGTGACCGGCATGGTTGTGG+CGG | 0.607116 | 7.4:+30255315 | None:intergenic |
| CACCGCTGGGATTCAATCAG+TGG | 0.607722 | 7.4:-30255173 | MS.gene016231:CDS |
| TTAAGCCTGCCAATCAAACA+TGG | 0.610865 | 7.4:+30268470 | None:intergenic |
| CAGAAATCAGAACCAATGGA+AGG | 0.613736 | 7.4:-30261555 | MS.gene016231:CDS |
| GGAGCCGCAGTCGCCTGTGG+TGG | 0.616723 | 7.4:+30255373 | None:intergenic |
| GTAGACATATCATAGGTGAC+CGG | 0.623164 | 7.4:+30255301 | None:intergenic |
| GGTTGGTGGTAGGTCCGCAA+AGG | 0.624070 | 7.4:+30255339 | None:intergenic |
| ACCACCACCACAGGCGACTG+CGG | 0.626087 | 7.4:-30255377 | MS.gene016231:CDS |
| ACCAAACTTTACATGTAAAG+GGG | 0.633441 | 7.4:+30271771 | None:intergenic |
| GGAATACGAAGCAGATGATG+AGG | 0.637518 | 7.4:+30255096 | None:intergenic |
| TTGGTGAAAGCAGATATCAC+TGG | 0.649482 | 7.4:-30271730 | MS.gene016231:CDS |
| TTGATTAATACTAGTAACAG+CGG | 0.656890 | 7.4:+30268406 | None:intergenic |
| GATGATGAGGCATTGGACGA+TGG | 0.665343 | 7.4:+30255109 | None:intergenic |
| AGCCTGCCAATCAAACATGG+CGG | 0.665798 | 7.4:+30268473 | None:intergenic |
| AAAGCCACATTAATTCAACT+GGG | 0.678701 | 7.4:-30255420 | MS.gene016231:CDS |
| CACTGATTGAATCCCAGCGG+TGG | 0.680808 | 7.4:+30255174 | None:intergenic |
| CATATCATAGGTGACCGGCA+TGG | 0.691704 | 7.4:+30255306 | None:intergenic |
| CATTGTCCATGCAACAACAG+AGG | 0.694061 | 7.4:-30255252 | MS.gene016231:CDS |
| TGAGCAGCTAAGTATTGACG+TGG | 0.699058 | 7.4:+30255217 | None:intergenic |
| TGCCACTGATTGAATCCCAG+CGG | 0.713863 | 7.4:+30255171 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 30255081 | 30271828 | 30255081 | ID=MS.gene016231 |
| chr7.4 | mRNA | 30255081 | 30271828 | 30255081 | ID=MS.gene016231.t1;Parent=MS.gene016231 |
| chr7.4 | exon | 30271639 | 30271828 | 30271639 | ID=MS.gene016231.t1.exon1;Parent=MS.gene016231.t1 |
| chr7.4 | CDS | 30271639 | 30271828 | 30271639 | ID=cds.MS.gene016231.t1;Parent=MS.gene016231.t1 |
| chr7.4 | exon | 30268417 | 30268533 | 30268417 | ID=MS.gene016231.t1.exon2;Parent=MS.gene016231.t1 |
| chr7.4 | CDS | 30268417 | 30268533 | 30268417 | ID=cds.MS.gene016231.t1;Parent=MS.gene016231.t1 |
| chr7.4 | exon | 30261529 | 30261651 | 30261529 | ID=MS.gene016231.t1.exon3;Parent=MS.gene016231.t1 |
| chr7.4 | CDS | 30261529 | 30261651 | 30261529 | ID=cds.MS.gene016231.t1;Parent=MS.gene016231.t1 |
| chr7.4 | exon | 30258343 | 30258449 | 30258343 | ID=MS.gene016231.t1.exon4;Parent=MS.gene016231.t1 |
| chr7.4 | CDS | 30258343 | 30258449 | 30258343 | ID=cds.MS.gene016231.t1;Parent=MS.gene016231.t1 |
| chr7.4 | exon | 30255081 | 30255476 | 30255081 | ID=MS.gene016231.t1.exon5;Parent=MS.gene016231.t1 |
| chr7.4 | CDS | 30255081 | 30255476 | 30255081 | ID=cds.MS.gene016231.t1;Parent=MS.gene016231.t1 |
| Gene Sequence |
| Protein sequence |
