AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016232

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016232.t1 MTR_7g075230 97.436 195 4 1 1 195 1 194 6.52e-141 391
MS.gene016232.t1 MTR_8g036105 62.051 195 62 6 4 194 10 196 9.92e-68 206
MS.gene016232.t1 MTR_8g036085 72.642 106 27 2 11 116 22 125 4.96e-52 167
MS.gene016232.t1 MTR_7g074990 67.619 105 32 2 12 116 20 122 3.99e-46 152
MS.gene016232.t1 MTR_7g096610 65.546 119 36 4 10 125 6 122 1.78e-45 150
MS.gene016232.t1 MTR_1g070205 54.225 142 60 4 12 151 12 150 7.04e-45 149
MS.gene016232.t1 MTR_7g096530 65.487 113 37 2 4 116 6 116 2.68e-44 147
MS.gene016232.t1 MTR_1g070220 58.065 124 50 2 10 133 13 134 1.05e-41 140
MS.gene016232.t1 MTR_6g027710 62.385 109 37 3 12 120 40 144 1.06e-39 135
MS.gene016232.t1 MTR_5g080470 61.616 99 36 2 10 108 5 101 2.73e-39 133
MS.gene016232.t1 MTR_5g083230 46.386 166 76 5 9 164 3 165 3.94e-37 127
MS.gene016232.t1 MTR_3g071590 42.857 161 78 3 6 155 2 159 3.92e-35 121
MS.gene016232.t1 MTR_7g033800 53.043 115 51 2 12 126 11 122 8.52e-33 116
MS.gene016232.t1 MTR_2g068760 52.632 114 45 3 8 115 3 113 1.77e-32 114
MS.gene016232.t1 MTR_3g031660 51.402 107 49 2 9 115 4 107 4.47e-32 114
MS.gene016232.t1 MTR_4g105170 58.586 99 38 2 10 108 9 104 1.32e-31 113
MS.gene016232.t1 MTR_3g077240 55.660 106 44 2 10 115 8 110 3.31e-31 112
MS.gene016232.t1 MTR_6g005070 53.704 108 47 2 12 119 9 113 3.99e-31 110
MS.gene016232.t1 MTR_4g083680 54.902 102 43 2 12 113 15 113 5.65e-31 111
MS.gene016232.t1 MTR_4g060950 42.958 142 67 3 11 141 23 161 7.60e-31 111
MS.gene016232.t1 MTR_6g027700 50.893 112 53 2 8 119 3 112 1.36e-30 111
MS.gene016232.t1 MTR_5g083010 51.923 104 47 2 13 116 12 112 2.35e-30 110
MS.gene016232.t1 MTR_5g083960 43.038 158 78 5 12 162 39 191 3.84e-29 107
MS.gene016232.t1 MTR_2g093310 49.524 105 50 2 11 115 32 133 7.76e-29 106
MS.gene016232.t1 MTR_3g452660 52.778 108 48 2 12 119 7 111 1.63e-28 108
MS.gene016232.t1 MTR_6g005080 51.923 104 47 2 12 115 10 110 2.57e-28 103
MS.gene016232.t1 MTR_3g073690 50.926 108 50 2 9 116 6 110 6.27e-28 104
MS.gene016232.t1 MTR_3g071420 50.000 100 47 2 13 112 15 111 1.88e-27 102
MS.gene016232.t1 MTR_5g017950 52.381 105 47 2 12 116 8 109 3.50e-27 105
MS.gene016232.t1 MTR_1g095850 50.485 103 48 2 13 115 73 172 1.07e-26 102
MS.gene016232.t1 MTR_3g094690 50.962 104 48 2 12 115 47 147 1.36e-26 102
MS.gene016232.t1 MTR_3g094690 50.962 104 47 3 12 115 47 146 6.75e-25 97.4
MS.gene016232.t1 MTR_5g075020 53.846 104 45 2 12 115 43 143 7.27e-23 92.0
MS.gene016232.t1 MTR_5g075020 53.846 104 45 2 12 115 47 147 1.11e-22 91.7
MS.gene016232.t1 MTR_5g093010 42.453 106 58 2 11 116 4 106 2.15e-22 88.6
MS.gene016232.t1 MTR_8g040900 49.550 111 53 2 12 122 7 114 1.33e-21 88.6
MS.gene016232.t1 MTR_7g028905 38.462 104 61 2 13 116 6 106 4.00e-20 81.6
MS.gene016232.t1 MTR_8g079660 39.048 105 61 2 12 116 8 109 1.37e-17 78.6
MS.gene016232.t1 MTR_8g079620 40.952 105 59 2 12 116 8 109 1.73e-16 75.1
MS.gene016232.t1 MTR_2g100020 37.143 105 63 2 13 117 20 121 7.57e-16 73.9
MS.gene016232.t1 MTR_8g017090 37.736 106 63 2 13 118 13 115 2.81e-15 72.8
MS.gene016232.t1 MTR_6g011250 39.623 106 60 3 13 117 10 112 3.60e-15 70.5
MS.gene016232.t1 MTR_8g079580 36.538 104 64 1 12 115 8 109 9.02e-15 70.5
MS.gene016232.t1 MTR_4g107450 37.143 105 63 2 13 117 11 112 3.69e-14 69.7
MS.gene016232.t1 MTR_6g011230 33.929 112 70 3 1 112 1 108 2.34e-13 66.2
MS.gene016232.t1 MTR_6g011200 34.906 106 58 5 13 110 10 112 2.76e-12 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016232.t1 AT2G45410 71.429 119 31 3 15 132 19 135 4.96e-53 168
MS.gene016232.t1 AT4G00210 63.971 136 43 3 10 141 9 142 2.31e-52 167
MS.gene016232.t1 AT2G45420 75.238 105 24 2 15 119 40 142 1.49e-48 159
MS.gene016232.t1 AT4G00220 69.524 105 30 2 15 119 20 122 1.04e-45 150
MS.gene016232.t1 AT3G03760 68.468 111 32 3 12 122 51 158 5.58e-45 150
MS.gene016232.t1 AT3G58190 57.851 121 49 2 12 132 11 129 7.65e-45 148
MS.gene016232.t1 AT2G42430 56.934 137 48 4 10 137 13 147 4.29e-44 147
MS.gene016232.t1 AT2G42440 55.200 125 49 3 10 129 5 127 1.08e-42 143
MS.gene016232.t1 AT5G06080 48.026 152 64 4 10 151 5 151 1.04e-40 136
MS.gene016232.t1 AT2G31310 58.879 107 42 2 10 116 5 109 1.64e-39 134
MS.gene016232.t1 AT2G30130 50.407 123 57 3 6 128 2 120 1.28e-33 119
MS.gene016232.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.15e-31 112
MS.gene016232.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.15e-31 112
MS.gene016232.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.15e-31 112
MS.gene016232.t1 AT5G63090 57.143 105 42 2 12 116 11 112 2.15e-31 112
MS.gene016232.t1 AT1G16530 49.153 118 55 2 11 125 13 128 2.53e-31 112
MS.gene016232.t1 AT3G27650 53.846 104 45 2 12 115 39 139 3.05e-30 109
MS.gene016232.t1 AT1G07900 40.741 162 82 5 11 164 32 187 1.61e-29 108
MS.gene016232.t1 AT3G11090 41.290 155 83 5 13 163 12 162 1.93e-29 107
MS.gene016232.t1 AT2G28500 50.962 104 48 2 12 115 55 155 9.16e-29 107
MS.gene016232.t1 AT5G66870 49.635 137 54 5 12 136 7 140 7.02e-28 107
MS.gene016232.t1 AT1G31320 53.333 90 39 2 12 101 13 99 1.00e-27 103
MS.gene016232.t1 AT3G26660 44.762 105 55 2 13 117 6 107 2.61e-27 100
MS.gene016232.t1 AT3G26620 44.762 105 55 2 13 117 6 107 4.78e-27 99.8
MS.gene016232.t1 AT2G23660 47.115 104 52 2 12 115 5 105 4.46e-25 99.4
MS.gene016232.t1 AT2G23660 47.115 104 52 2 12 115 5 105 4.46e-25 99.4
MS.gene016232.t1 AT2G23660 47.115 104 52 2 12 115 5 105 4.46e-25 99.4
MS.gene016232.t1 AT2G30340 48.077 104 51 2 12 115 52 152 1.05e-24 97.8
MS.gene016232.t1 AT2G30340 48.077 104 51 2 12 115 53 153 1.25e-24 97.4
MS.gene016232.t1 AT3G50510 43.636 110 59 2 7 116 7 113 4.76e-23 91.7
MS.gene016232.t1 AT3G50510 43.636 110 59 2 7 116 7 113 4.76e-23 91.7
MS.gene016232.t1 AT2G40470 52.885 104 46 2 12 115 20 120 5.99e-22 89.0
MS.gene016232.t1 AT2G40470 52.885 104 46 2 12 115 45 145 6.17e-22 89.4
MS.gene016232.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.81e-22 88.6
MS.gene016232.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.81e-22 88.6
MS.gene016232.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.81e-22 88.6
MS.gene016232.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.81e-22 88.6
MS.gene016232.t1 AT1G65620 52.381 105 47 2 12 116 9 110 7.81e-22 88.6
MS.gene016232.t1 AT1G72980 35.772 123 74 3 9 129 8 127 1.81e-15 72.0
MS.gene016232.t1 AT5G35900 33.043 115 74 2 13 127 6 117 4.05e-15 70.9
MS.gene016232.t1 AT3G13850 33.898 118 75 2 13 130 37 151 6.36e-15 71.2
MS.gene016232.t1 AT3G47870 29.907 107 72 2 11 117 35 138 2.52e-12 64.7
MS.gene016232.t1 AT1G06280 41.558 77 42 2 13 89 25 98 2.61e-12 63.2

Find 37 sgRNAs with CRISPR-Local

Find 195 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGGGCTACTGACCCTGTTTA+TGG 0.239262 7.4:+30281499 MS.gene016232:CDS
TTAGAGAGAGAGATGGATTT+GGG 0.267071 7.4:+30281244 None:intergenic
AAGGCAAAGCTTGGAATTCC+TGG 0.322902 7.4:-30284530 None:intergenic
TTTAGAGAGAGAGATGGATT+TGG 0.338238 7.4:+30281243 None:intergenic
GGGTGTGGGGATGACGGTGG+TGG 0.353668 7.4:+30281265 MS.gene016232:CDS
AGAGAGATGGATTTGGGGTG+TGG 0.373011 7.4:+30281250 None:intergenic
GTCATGAAGTTGTTGATTAT+AGG 0.378812 7.4:-30284508 None:intergenic
TGACCTGATGACTCAAAATA+TGG 0.381006 7.4:-30281349 None:intergenic
CTTTGAGGCTGTTGAAATTG+AGG 0.406522 7.4:-30284339 None:intergenic
TGAGCAACACAACCATAAAC+AGG 0.407283 7.4:-30281511 None:intergenic
GAGAGATGGATTTGGGGTGT+GGG 0.427932 7.4:+30281251 None:intergenic
GCTGCAGTTCATAAGGTGTT+TGG 0.429613 7.4:+30281388 MS.gene016232:CDS
TTGGGGTGTGGGGATGACGG+TGG 0.437721 7.4:+30281262 MS.gene016232:CDS
CCTCTTTGCTCTTCAACAAC+AGG 0.440953 7.4:+30281534 MS.gene016232:CDS
GCATCAAGACGCTTATGAAC+TGG 0.441293 7.4:-30281445 None:intergenic
GATTTGGGGTGTGGGGATGA+CGG 0.456260 7.4:+30281259 MS.gene016232:CDS
AAAATTCTCAAGGCAAAGCT+TGG 0.480367 7.4:-30284539 None:intergenic
TGATTATAGGAATAGTATGA+TGG 0.495256 7.4:-30284495 None:intergenic
TCTGCTATGAGGCTCTTGCT+AGG 0.495453 7.4:+30281479 MS.gene016232:CDS
CTGCTATGAGGCTCTTGCTA+GGG 0.502068 7.4:+30281480 MS.gene016232:CDS
GCGATTCAATATTGAAAGAC+GGG 0.503238 7.4:-30284304 None:intergenic
AGTAAGGATGAATGCAAAGT+TGG 0.505000 7.4:-30284375 None:intergenic
TCTTACATGTCCATGCACTT+TGG 0.510678 7.4:+30284426 MS.gene016232:CDS
GGTGTGGGGATGACGGTGGT+GGG 0.515639 7.4:+30281266 MS.gene016232:CDS
TGAAAGACGGGCTTGTACAT+AGG 0.516839 7.4:-30284292 None:intergenic
TGTTGTTACTCTCTGCTATG+AGG 0.534536 7.4:+30281468 MS.gene016232:CDS
AGAGATGGATTTGGGGTGTG+GGG 0.541784 7.4:+30281252 None:intergenic
GGTGATATTGAAGAGCTTTG+AGG 0.552521 7.4:-30284354 None:intergenic
TTGAGAAGAAAGTGTGTGAA+AGG 0.553888 7.4:+30281310 MS.gene016232:CDS
TGATGACAAATGATGAAGTA+AGG 0.555832 7.4:-30284391 None:intergenic
GATGACGGTGGTGGGCCTTG+TGG 0.575954 7.4:+30281274 MS.gene016232:CDS
TGCGATTCAATATTGAAAGA+CGG 0.576843 7.4:-30284305 None:intergenic
TAGAGAGAGAGATGGATTTG+GGG 0.584166 7.4:+30281245 None:intergenic
AAGAGGATGACCAAAGTGCA+TGG 0.603188 7.4:-30284436 None:intergenic
TAATCAACAACTTCATGACC+AGG 0.619495 7.4:+30284512 MS.gene016232:CDS
CCTGTTGTTGAAGAGCAAAG+AGG 0.642330 7.4:-30281534 None:intergenic
GAGCAACACAACCATAAACA+GGG 0.707824 7.4:-30281510 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATGACATATATAAAT+AGG - chr7.4:30281908-30281927 None:intergenic 10.0%
!! ATATAATTGTATTTAAACTA+TGG - chr7.4:30284231-30284250 None:intergenic 10.0%
!! TATAATTGTATTTAAACTAT+GGG - chr7.4:30284230-30284249 None:intergenic 10.0%
!!! AAATAGGATTTATATTATTT+TGG - chr7.4:30281892-30281911 None:intergenic 10.0%
!!! ATTTTATCAATAATATTCTT+TGG + chr7.4:30281938-30281957 MS.gene016232:intron 10.0%
!!! ATTTTGATATCAATAATTTA+GGG - chr7.4:30283837-30283856 None:intergenic 10.0%
!!! TATTATTTTGGAAAATAAAT+AGG - chr7.4:30281880-30281899 None:intergenic 10.0%
!!! TATTTTGATATCAATAATTT+AGG - chr7.4:30283838-30283857 None:intergenic 10.0%
!! ATAGATAACTAATAGTTTAA+GGG + chr7.4:30284027-30284046 MS.gene016232:intron 15.0%
!! ATTCAAGAAAAACTTATAAT+AGG + chr7.4:30281757-30281776 MS.gene016232:intron 15.0%
!! CAGAAAAAAAGAAAAAAAAA+AGG + chr7.4:30282069-30282088 MS.gene016232:intron 15.0%
!! TAAATAGGATTTATATTGTT+TGG - chr7.4:30281865-30281884 None:intergenic 15.0%
!! TAAATGAATAAATGATCAAT+AGG - chr7.4:30283989-30284008 None:intergenic 15.0%
!! TAATTCGTATATATATAACT+TGG + chr7.4:30281637-30281656 MS.gene016232:intron 15.0%
!! TATAGATAACTAATAGTTTA+AGG + chr7.4:30284026-30284045 MS.gene016232:intron 15.0%
!! TATATATATACGAATTAGTT+AGG - chr7.4:30281634-30281653 None:intergenic 15.0%
!! TATTCATTTATATGGTATAT+GGG + chr7.4:30283999-30284018 MS.gene016232:intron 15.0%
!! TGATCATTTATTCATTTATA+TGG + chr7.4:30283991-30284010 MS.gene016232:intron 15.0%
!! TTATATACTTGATTAATTAC+AGG + chr7.4:30284245-30284264 MS.gene016232:intron 15.0%
!! TTATTCATTTATATGGTATA+TGG + chr7.4:30283998-30284017 MS.gene016232:intron 15.0%
!!! AATAAATAAGGGTATAATAA+TGG + chr7.4:30282917-30282936 MS.gene016232:intron 15.0%
!!! ATGCAATGTAAAATAAATTT+TGG - chr7.4:30282300-30282319 None:intergenic 15.0%
!!! ATTTTGAGAAAAAGAAAAAA+TGG + chr7.4:30282983-30283002 MS.gene016232:intron 15.0%
!!! CTTTTTTTTTTCTTTTTTTC+TGG - chr7.4:30282071-30282090 None:intergenic 15.0%
!! AAAAATAATAAATGCTGCAT+TGG + chr7.4:30282942-30282961 MS.gene016232:intron 20.0%
!! AAATGTTAATAGTACCTTTA+GGG + chr7.4:30283234-30283253 MS.gene016232:intron 20.0%
!! AAGTTATATTTGTAGATTTC+TGG + chr7.4:30282575-30282594 MS.gene016232:intron 20.0%
!! AATAAATGATCAATAGGTTA+GGG - chr7.4:30283983-30284002 None:intergenic 20.0%
!! AGAAGAATGGTTTAATAAAT+TGG + chr7.4:30281962-30281981 MS.gene016232:intron 20.0%
!! AGTTATATTTGTAGATTTCT+GGG + chr7.4:30282576-30282595 MS.gene016232:intron 20.0%
!! ATGACTTACATAATGTTATT+TGG - chr7.4:30283368-30283387 None:intergenic 20.0%
!! GGATGAAATTCAATAAATAA+GGG + chr7.4:30282906-30282925 MS.gene016232:intron 20.0%
!! TAAGTCACACAAAAAAAAAA+GGG + chr7.4:30283211-30283230 MS.gene016232:intron 20.0%
!! TGGATGAAATTCAATAAATA+AGG + chr7.4:30282905-30282924 MS.gene016232:intron 20.0%
!! TGTATAAATACAACTATGTT+AGG - chr7.4:30283586-30283605 None:intergenic 20.0%
!! TTTCTAATTCACAAAAGATA+AGG - chr7.4:30283656-30283675 None:intergenic 20.0%
!!! AAAAATTTTCCCAAAGAAAA+GGG + chr7.4:30282684-30282703 MS.gene016232:intron 20.0%
!!! AAGAAAATGTTTCCATTTTT+GGG - chr7.4:30284089-30284108 None:intergenic 20.0%
!!! AGTTTACTAAATTTTATTCC+CGG + chr7.4:30282638-30282657 MS.gene016232:intron 20.0%
!!! ATTAATTGTGTTGATTTTGA+TGG + chr7.4:30282822-30282841 MS.gene016232:intron 20.0%
!!! TAAGAAAATGTTTCCATTTT+TGG - chr7.4:30284090-30284109 None:intergenic 20.0%
!!! TATATTGCTTTAATTTGTTC+AGG - chr7.4:30282176-30282195 None:intergenic 20.0%
!!! TCAAAGGCATAATATAATTA+AGG + chr7.4:30282448-30282467 MS.gene016232:intron 20.0%
!!! TGACTTACATAATGTTATTT+GGG - chr7.4:30283367-30283386 None:intergenic 20.0%
! AAACGACTATTATTTAGAGA+CGG - chr7.4:30282773-30282792 None:intergenic 25.0%
! AATATTCTTTGGTAGAAGAA+TGG + chr7.4:30281949-30281968 MS.gene016232:intron 25.0%
! AATTTCACTTGATTAAAGAG+TGG - chr7.4:30283474-30283493 None:intergenic 25.0%
! AATTTGTACATTCAAAGCAT+TGG + chr7.4:30283294-30283313 MS.gene016232:intron 25.0%
! ACAAAAGATAAGGTAACAAA+CGG - chr7.4:30283646-30283665 None:intergenic 25.0%
! AGAAGAAAGATCATATTCAT+AGG + chr7.4:30282114-30282133 MS.gene016232:intron 25.0%
! ATTATTCCTAGGAATGAATT+TGG + chr7.4:30283139-30283158 MS.gene016232:intron 25.0%
! ATTCCTTAGAACATAAAGAT+GGG - chr7.4:30283119-30283138 None:intergenic 25.0%
! ATTTCACTTGATTAAAGAGT+GGG - chr7.4:30283473-30283492 None:intergenic 25.0%
! CCACTAACTACAATTAATAA+GGG - chr7.4:30282874-30282893 None:intergenic 25.0%
! CTAATACATTACACAACAAT+GGG + chr7.4:30283899-30283918 MS.gene016232:intron 25.0%
! CTCTAAATAATAGTCGTTTA+AGG + chr7.4:30282774-30282793 MS.gene016232:intron 25.0%
! GAAATGTTAATAGTACCTTT+AGG + chr7.4:30283233-30283252 MS.gene016232:intron 25.0%
! GAAGAAAGATCATATTCATA+GGG + chr7.4:30282115-30282134 MS.gene016232:intron 25.0%
! GAATAAATGATCAATAGGTT+AGG - chr7.4:30283984-30284003 None:intergenic 25.0%
! GTAAGTCACACAAAAAAAAA+AGG + chr7.4:30283210-30283229 MS.gene016232:intron 25.0%
! TAATACATTACACAACAATG+GGG + chr7.4:30283900-30283919 MS.gene016232:intron 25.0%
! TATTCCTTAGAACATAAAGA+TGG - chr7.4:30283120-30283139 None:intergenic 25.0%
! TCCACTAACTACAATTAATA+AGG - chr7.4:30282875-30282894 None:intergenic 25.0%
! TCTAAAGAAAATTACCAGTT+AGG - chr7.4:30282038-30282057 None:intergenic 25.0%
! TGATTATAGGAATAGTATGA+TGG - chr7.4:30284498-30284517 None:intergenic 25.0%
! TTTAACGCTATAATATTCCT+GGG - chr7.4:30283060-30283079 None:intergenic 25.0%
!! AGTGTTTAGGATAATAATGT+TGG - chr7.4:30283938-30283957 None:intergenic 25.0%
!! GAAAAATTTTCCCAAAGAAA+AGG + chr7.4:30282683-30282702 MS.gene016232:intron 25.0%
!! TTCTAAGGAATATTATTCCT+AGG + chr7.4:30283128-30283147 MS.gene016232:intron 25.0%
!!! CATCTTTTTTCTAAATACCT+TGG + chr7.4:30283084-30283103 MS.gene016232:intron 25.0%
!!! TAGTATGATGGATTTTCAAA+AGG - chr7.4:30284486-30284505 None:intergenic 25.0%
!!! TGATATTGATCATTTTGAAG+AGG - chr7.4:30284456-30284475 None:intergenic 25.0%
!!! TGCTTTTGATACTTTAAAGA+TGG + chr7.4:30283498-30283517 MS.gene016232:intron 25.0%
AAGAGACAATTATTGTGAGA+CGG + chr7.4:30283008-30283027 MS.gene016232:intron 30.0%
AATGTATATTCTCAAGGTGT+TGG + chr7.4:30281793-30281812 MS.gene016232:intron 30.0%
ACTTGATTAATTACAGGTTG+TGG + chr7.4:30284251-30284270 MS.gene016232:intron 30.0%
AGTTTACCAAATTCATTCCT+AGG - chr7.4:30283148-30283167 None:intergenic 30.0%
ATGCAAATAAGTATCCTAAC+TGG + chr7.4:30282021-30282040 MS.gene016232:intron 30.0%
ATGTTTGATCGTGCATTTAA+TGG - chr7.4:30282475-30282494 None:intergenic 30.0%
ATTCCCATCTTTATGTTCTA+AGG + chr7.4:30283113-30283132 MS.gene016232:intron 30.0%
CAAAAGATAAGGTAACAAAC+GGG - chr7.4:30283645-30283664 None:intergenic 30.0%
CATCATATTCCAATATTGTG+TGG - chr7.4:30283405-30283424 None:intergenic 30.0%
CCACTAAAAGGAAACTAATA+AGG - chr7.4:30281832-30281851 None:intergenic 30.0%
CCCTTATTAATTGTAGTTAG+TGG + chr7.4:30282871-30282890 MS.gene016232:intron 30.0%
GCTAATACATTACACAACAA+TGG + chr7.4:30283898-30283917 MS.gene016232:intron 30.0%
GGCAGAAAAAATGAAAACTA+AGG - chr7.4:30281613-30281632 None:intergenic 30.0%
GTTTAACGCTATAATATTCC+TGG - chr7.4:30283061-30283080 None:intergenic 30.0%
TGATGACAAATGATGAAGTA+AGG - chr7.4:30284394-30284413 None:intergenic 30.0%
TGCGATTCAATATTGAAAGA+CGG - chr7.4:30284308-30284327 None:intergenic 30.0%
TGTATTAGCGATGACATTAA+AGG - chr7.4:30283887-30283906 None:intergenic 30.0%
TGTATTGAGTATGTTCTGTT+GGG + chr7.4:30282325-30282344 MS.gene016232:intron 30.0%
TTGTATTGAGTATGTTCTGT+TGG + chr7.4:30282324-30282343 MS.gene016232:intron 30.0%
! AAGATGGGAATTTTATTCCA+AGG - chr7.4:30283104-30283123 None:intergenic 30.0%
! AATATTGGAATATGATGACG+AGG + chr7.4:30283408-30283427 MS.gene016232:intron 30.0%
! ATATTGGAATATGATGACGA+GGG + chr7.4:30283409-30283428 MS.gene016232:intron 30.0%
! ATTTCTGGGAATAAAACGAT+AGG + chr7.4:30282590-30282609 MS.gene016232:intron 30.0%
! CAGCTTTAGTATTAGTGTTT+AGG - chr7.4:30283951-30283970 None:intergenic 30.0%
! GTCATGAAGTTGTTGATTAT+AGG - chr7.4:30284511-30284530 None:intergenic 30.0%
!! CCTTATTAGTTTCCTTTTAG+TGG + chr7.4:30281829-30281848 MS.gene016232:intron 30.0%
!! TAACTTGGTGCTTTAAAAAC+TGG + chr7.4:30281652-30281671 MS.gene016232:intron 30.0%
!!! GGGAGTAATTCCTATTTTTA+TGG + chr7.4:30283032-30283051 MS.gene016232:intron 30.0%
!!! TGTGTACTTTTCTAAAGTAG+TGG + chr7.4:30282232-30282251 MS.gene016232:intron 30.0%
AAATAAAGTTCCCATGGAGA+TGG + chr7.4:30282711-30282730 MS.gene016232:intron 35.0%
AAGTTTCATGGTAAGTCTAG+TGG - chr7.4:30282505-30282524 None:intergenic 35.0%
AATAAAGTTCCCATGGAGAT+GGG + chr7.4:30282712-30282731 MS.gene016232:intron 35.0%
AGTAAGGATGAATGCAAAGT+TGG - chr7.4:30284378-30284397 None:intergenic 35.0%
AGTTAGTGGAGTAGTTAAGT+TGG + chr7.4:30282885-30282904 MS.gene016232:intron 35.0%
ATGTGAGGAACTAAGTTTCA+TGG - chr7.4:30282517-30282536 None:intergenic 35.0%
CATGTTAAGCAACCCAAAAA+TGG + chr7.4:30284074-30284093 MS.gene016232:intron 35.0%
GAATTTATTCCCATCTCCAT+GGG - chr7.4:30282724-30282743 None:intergenic 35.0%
GACTATTATTTAGAGACGGA+GGG - chr7.4:30282769-30282788 None:intergenic 35.0%
GCAGCTAATGTATATTCTCA+AGG + chr7.4:30281787-30281806 MS.gene016232:intron 35.0%
GCGATTCAATATTGAAAGAC+GGG - chr7.4:30284307-30284326 None:intergenic 35.0%
GTAATCATCACATGAATGTG+AGG - chr7.4:30282532-30282551 None:intergenic 35.0%
TAATCAACAACTTCATGACC+AGG + chr7.4:30284512-30284531 MS.gene016232:CDS 35.0%
TCATGTCAACCACACAATAT+TGG + chr7.4:30283393-30283412 MS.gene016232:intron 35.0%
TGAATTTATTCCCATCTCCA+TGG - chr7.4:30282725-30282744 None:intergenic 35.0%
TGACCTGATGACTCAAAATA+TGG - chr7.4:30281352-30281371 None:intergenic 35.0%
TTCCTGGGAACCATAAAAAT+AGG - chr7.4:30283045-30283064 None:intergenic 35.0%
TTGCTTAACATGTGTCCTAA+GGG - chr7.4:30284066-30284085 None:intergenic 35.0%
! AAGGTATAAGAGTATGAGAG+CGG + chr7.4:30282088-30282107 MS.gene016232:intron 35.0%
! AATTTTCCCAAAGAAAAGGG+TGG + chr7.4:30282687-30282706 MS.gene016232:intron 35.0%
! AGTCGTTTAAGGTTTATGCA+CGG + chr7.4:30282785-30282804 MS.gene016232:intron 35.0%
! ATTTTCCCAAAGAAAAGGGT+GGG + chr7.4:30282688-30282707 MS.gene016232:intron 35.0%
! GGGAAAATTTTTCTATTCCC+AGG - chr7.4:30282676-30282695 None:intergenic 35.0%
! TATTTCCCACCCTTTTCTTT+GGG - chr7.4:30282696-30282715 None:intergenic 35.0%
! TCATTTTGCTGCAGTTCATA+AGG + chr7.4:30281381-30281400 MS.gene016232:CDS 35.0%
! TTATTTCCCACCCTTTTCTT+TGG - chr7.4:30282697-30282716 None:intergenic 35.0%
! TTGAGAAGAAAGTGTGTGAA+AGG + chr7.4:30281310-30281329 MS.gene016232:CDS 35.0%
!! AATCATTAACTTGTGCCTTG+AGG + chr7.4:30284188-30284207 MS.gene016232:intron 35.0%
!! AGCTCTGGTTAGGTTTTATA+TGG + chr7.4:30282363-30282382 MS.gene016232:intron 35.0%
!! ATCATTAACTTGTGCCTTGA+GGG + chr7.4:30284189-30284208 MS.gene016232:intron 35.0%
!! GATGGATTTTCAAAAGGTGT+TGG - chr7.4:30284480-30284499 None:intergenic 35.0%
!! TATTTTGAGTCATCAGGTCA+AGG + chr7.4:30281352-30281371 MS.gene016232:CDS 35.0%
!!! TTCCTATTTTTATGGTTCCC+AGG + chr7.4:30283040-30283059 MS.gene016232:intron 35.0%
AAAAACTTGCAAGCACCACA+AGG - chr7.4:30281292-30281311 None:intergenic 40.0%
AAAACTGGTAACCACTAACC+TGG + chr7.4:30281667-30281686 MS.gene016232:intron 40.0%
ACATAACACTACCGTGAAAG+GGG - chr7.4:30282413-30282432 None:intergenic 40.0%
AGACAATTATTGTGAGACGG+AGG + chr7.4:30283011-30283030 MS.gene016232:intron 40.0%
AGGTTTATGCACGGGTATTA+AGG + chr7.4:30282794-30282813 MS.gene016232:intron 40.0%
CACATAACACTACCGTGAAA+GGG - chr7.4:30282414-30282433 None:intergenic 40.0%
CGACTATTATTTAGAGACGG+AGG - chr7.4:30282770-30282789 None:intergenic 40.0%
GACAATTATTGTGAGACGGA+GGG + chr7.4:30283012-30283031 MS.gene016232:intron 40.0%
GAGCAACACAACCATAAACA+GGG - chr7.4:30281513-30281532 None:intergenic 40.0%
GGACAAAAGAGACCACTAAA+AGG - chr7.4:30281844-30281863 None:intergenic 40.0%
GGTGATATTGAAGAGCTTTG+AGG - chr7.4:30284357-30284376 None:intergenic 40.0%
GTTGCTTAACATGTGTCCTA+AGG - chr7.4:30284067-30284086 None:intergenic 40.0%
TAAAGAGTGGGTGATAGAGT+GGG - chr7.4:30283461-30283480 None:intergenic 40.0%
TCAAGTGTCACAACAGTGTT+AGG + chr7.4:30283618-30283637 MS.gene016232:intron 40.0%
TCTTACATGTCCATGCACTT+TGG + chr7.4:30284426-30284445 MS.gene016232:CDS 40.0%
TGAGCAACACAACCATAAAC+AGG - chr7.4:30281514-30281533 None:intergenic 40.0%
TGCATGTCTTGTGATCTCAA+AGG + chr7.4:30282432-30282451 MS.gene016232:intron 40.0%
TGGAACGAATGCATACACTA+AGG + chr7.4:30282383-30282402 MS.gene016232:intron 40.0%
TGTTGTTACTCTCTGCTATG+AGG + chr7.4:30281468-30281487 MS.gene016232:CDS 40.0%
TTAAAGAGTGGGTGATAGAG+TGG - chr7.4:30283462-30283481 None:intergenic 40.0%
TTTGACACATTTGTCAGCTC+TGG + chr7.4:30282348-30282367 MS.gene016232:intron 40.0%
! AGGTATAAGAGTATGAGAGC+GGG + chr7.4:30282089-30282108 MS.gene016232:intron 40.0%
! GTCGTTTAAGGTTTATGCAC+GGG + chr7.4:30282786-30282805 MS.gene016232:intron 40.0%
! TCCCAGGAACGTTTTATATC+CGG - chr7.4:30282660-30282679 None:intergenic 40.0%
!! AATGTTAACTTGTGCCCTCA+AGG - chr7.4:30284206-30284225 None:intergenic 40.0%
!! ATCCTTAAAGAGTGCCCTAA+AGG - chr7.4:30283251-30283270 None:intergenic 40.0%
!! CTTTGAGGCTGTTGAAATTG+AGG - chr7.4:30284342-30284361 None:intergenic 40.0%
!! GCACCATATTTTGAGTCATC+AGG + chr7.4:30281346-30281365 MS.gene016232:CDS 40.0%
!! TACCTTTAGGGCACTCTTTA+AGG + chr7.4:30283246-30283265 MS.gene016232:intron 40.0%
!! TTATGAACTTGTTACCCTCC+AGG - chr7.4:30281688-30281707 None:intergenic 40.0%
AAGAGGATGACCAAAGTGCA+TGG - chr7.4:30284439-30284458 None:intergenic 45.0%
AAGGCAAAGCTTGGAATTCC+TGG - chr7.4:30284533-30284552 None:intergenic 45.0%
CCCGGATATAAAACGTTCCT+GGG + chr7.4:30282656-30282675 MS.gene016232:intron 45.0%
CCTCTTTGCTCTTCAACAAC+AGG + chr7.4:30281534-30281553 MS.gene016232:CDS 45.0%
CCTGTTGTTGAAGAGCAAAG+AGG - chr7.4:30281537-30281556 None:intergenic 45.0%
GCACATAACACTACCGTGAA+AGG - chr7.4:30282415-30282434 None:intergenic 45.0%
GCATCAAGACGCTTATGAAC+TGG - chr7.4:30281448-30281467 None:intergenic 45.0%
GGTCATGTTAAGCAACCCTT+AGG + chr7.4:30284048-30284067 MS.gene016232:intron 45.0%
GGTGGGAAATAAAGTTCCCA+TGG + chr7.4:30282705-30282724 MS.gene016232:intron 45.0%
TCCCGGATATAAAACGTTCC+TGG + chr7.4:30282655-30282674 MS.gene016232:intron 45.0%
TGGGTGATAGAGTGGGATAT+TGG - chr7.4:30283454-30283473 None:intergenic 45.0%
! AGAGACGGAGGGAGTATAAT+AGG - chr7.4:30282758-30282777 None:intergenic 45.0%
! CACATTTGTCAGCTCTGGTT+AGG + chr7.4:30282353-30282372 MS.gene016232:intron 45.0%
! CCCAGGAACGTTTTATATCC+GGG - chr7.4:30282659-30282678 None:intergenic 45.0%
! GCTGCAGTTCATAAGGTGTT+TGG + chr7.4:30281388-30281407 MS.gene016232:CDS 45.0%
! GGTATAAGAGTATGAGAGCG+GGG + chr7.4:30282090-30282109 MS.gene016232:intron 45.0%
! TGAAAGACGGGCTTGTACAT+AGG - chr7.4:30284295-30284314 None:intergenic 45.0%
! TGGAATATGATGACGAGGGT+TGG + chr7.4:30283413-30283432 MS.gene016232:intron 45.0%
ACTAAGGCATGCCCCTTTCA+CGG + chr7.4:30282399-30282418 MS.gene016232:intron 50.0%
ACTGGTAACCACTAACCTGG+AGG + chr7.4:30281670-30281689 MS.gene016232:intron 50.0%
AGGGCTACTGACCCTGTTTA+TGG + chr7.4:30281499-30281518 MS.gene016232:CDS 50.0%
CTGCTATGAGGCTCTTGCTA+GGG + chr7.4:30281480-30281499 MS.gene016232:CDS 50.0%
CTGGTAACCACTAACCTGGA+GGG + chr7.4:30281671-30281690 MS.gene016232:intron 50.0%
CTTGTTACCCTCCAGGTTAG+TGG - chr7.4:30281681-30281700 None:intergenic 50.0%
TCTGCTATGAGGCTCTTGCT+AGG + chr7.4:30281479-30281498 MS.gene016232:CDS 50.0%
! GATTTGGGGTGTGGGGATGA+CGG + chr7.4:30281259-30281278 MS.gene016232:CDS 55.0%
! GGTGTGGGGATGACGGTGGT+GGG + chr7.4:30281266-30281285 MS.gene016232:CDS 65.0%
! TTGGGGTGTGGGGATGACGG+TGG + chr7.4:30281262-30281281 MS.gene016232:CDS 65.0%
!! GATGACGGTGGTGGGCCTTG+TGG + chr7.4:30281274-30281293 MS.gene016232:CDS 65.0%
! GGGTGTGGGGATGACGGTGG+TGG + chr7.4:30281265-30281284 MS.gene016232:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 30281256 30284554 30281256 ID=MS.gene016232
chr7.4 mRNA 30281256 30284554 30281256 ID=MS.gene016232.t1;Parent=MS.gene016232
chr7.4 exon 30281256 30281555 30281256 ID=MS.gene016232.t1.exon1;Parent=MS.gene016232.t1
chr7.4 CDS 30281256 30281555 30281256 ID=cds.MS.gene016232.t1;Parent=MS.gene016232.t1
chr7.4 exon 30284267 30284554 30284267 ID=MS.gene016232.t1.exon2;Parent=MS.gene016232.t1
chr7.4 CDS 30284267 30284554 30284267 ID=cds.MS.gene016232.t1;Parent=MS.gene016232.t1
Gene Sequence

>MS.gene016232

ATGGATTTGGGGTGTGGGGATGACGGTGGTGGGCCTTGTGGTGCTTGCAAGTTTTTGAGAAGAAAGTGTGTGAAAGGTTGTTGCATATTTGCACCATATTTTGAGTCATCAGGTCAAGGAAGTGCTCATTTTGCTGCAGTTCATAAGGTGTTTGGTGCTAGCAATGCTGCTAAGCTACTCACACGTATTCCAGTTCATAAGCGTCTTGATGCTGTTGTTACTCTCTGCTATGAGGCTCTTGCTAGGGCTACTGACCCTGTTTATGGTTGTGTTGCTCACCTCTTTGCTCTTCAACAACAGGTATACATACATATATCAGTTTTGTTTTGATACAGTGTATAACATTTGTTTCTTCCTTAGTTTTCATTTTTTCTGCCTAACTAATTCGTATATATATAACTTGGTGCTTTAAAAACTGGTAACCACTAACCTGGAGGGTAACAAGTTCATAAGACTTTATAGTAATAGATTAAAAAATATATATTAAAAATTACTTCACAAATTCAAGAAAAACTTATAATAGGTTCAAATGCAGCTAATGTATATTCTCAAGGTGTTGGAGTAATGCACTCTCCTTATTAGTTTCCTTTTAGTGGTCTCTTTTGTCCAAACAATATAAATCCTATTTATTTTCCAAAATAATATAAATCCTATTTATATATGTCATTTTTTATTAAATATTATTTTATCAATAATATTCTTTGGTAGAAGAATGGTTTAATAAATTGGTTAAATTATAATAAAGTTTATTAATTGTAAGAACTCATGCAAATAAGTATCCTAACTGGTAATTTTCTTTAGATATATAAGATCCAGAAAAAAAGAAAAAAAAAAGGTATAAGAGTATGAGAGCGGGGAAGAAGAAAGATCATATTCATAGGGATTAGTTGCAACATCAAAGCAATAATTTTTTTGTTCCTGAACAAATTAAAGCAATATAATTTTTTTTTGAGCAATAAAGTTAAATGCTGTAGAATGTGTACTTTTCTAAAGTAGTGGCATGACAAAAATCACTATTCACTATTATGCATTGCACGTATGCCAAAATTTATTTTACATTGCATAGTATTGTATTGAGTATGTTCTGTTGGGTTTGACACATTTGTCAGCTCTGGTTAGGTTTTATATGGAACGAATGCATACACTAAGGCATGCCCCTTTCACGGTAGTGTTATGTGCATGTCTTGTGATCTCAAAGGCATAATATAATTAAGGACCATTAAATGCACGATCAAACATATATTTACCACTAGACTTACCATGAAACTTAGTTCCTCACATTCATGTGATGATTACTGTGAAACTTGTGCGTTTGTTTCAAGTTATATTTGTAGATTTCTGGGAATAAAACGATAGGAATACATATGTCTATGTTTGTTACAAGTTTACTAAATTTTATTCCCGGATATAAAACGTTCCTGGGAATAGAAAAATTTTCCCAAAGAAAAGGGTGGGAAATAAAGTTCCCATGGAGATGGGAATAAATTCATGAGTAATTTCCTATTATACTCCCTCCGTCTCTAAATAATAGTCGTTTAAGGTTTATGCACGGGTATTAAGGAAATGATTAATTGTGTTGATTTTGATGGTAAAATTAGTATCATTTACTAAAACACCCTTATTAATTGTAGTTAGTGGAGTAGTTAAGTTGGATGAAATTCAATAAATAAGGGTATAATAATGGAAAAAAATAATAAATGCTGCATTGGTATTCTAAAGTGACAATTATTTTGAGAAAAAGAAAAAATGGTAAAGAGACAATTATTGTGAGACGGAGGGAGTAATTCCTATTTTTATGGTTCCCAGGAATATTATAGCGTTAAACAAACATCTTTTTTCTAAATACCTTGGAATAAAATTCCCATCTTTATGTTCTAAGGAATATTATTCCTAGGAATGAATTTGGTAAACTTGAAACAAACACATGATAAAAAGAAATAGTAGTCAACATTGTGTAAGTCACACAAAAAAAAAAGGGAAATGTTAATAGTACCTTTAGGGCACTCTTTAAGGATTTTAGATAGTAAGTTTTTATGAAAATTTGTACATTCAAAGCATTGGAAATTGAAAATTCTTCAAGAATGCTTTTAAAACACTCATTAGCAAGACCCAAATAACATTATGTAAGTCATTATGTTCATGTCAACCACACAATATTGGAATATGATGACGAGGGTTGGAACAATTTTTTTTTTCCAATATCCCACTCTATCACCCACTCTTTAATCAAGTGAAATTAATGTGCTTTTGATACTTTAAAGATGGTTCTCATATGAAGTGATGAGATTCACATGAATTTCACACAACAACAAGAGAGTACATTTGTTCCTAACATAGTTGTATTTATACATTAATTTAAAATTCAAGTGTCACAACAGTGTTAGGACCCGTTTGTTACCTTATCTTTTGTGAATTAGAAAAACATAAATTTTAGAAGTTCTATTTATTTATTACAAAAAAAATAATTAAAAATATCTGAAATTGCTTTGAAAAAAATGTTGTTTTTAGTAGCTTCTTTTGAAAATTATTTTTAATAATTAACATTTTTTAAATAAAAATGATTTTCTAAAAAGACAATCCCTAAATTATTGATATCAAAATAACTTTCTTATAATTAGTAACAAATGACCTTTAATGTCATCGCTAATACATTACACAACAATGGGGTAATGACAAACTCCAACATTATTATCCTAAACACTAATACTAAAGCTGTTTCATACGCCCTAACCTATTGATCATTTATTCATTTATATGGTATATGGGTTTTTATAGATAACTAATAGTTTAAGGGTCATGTTAAGCAACCCTTAGGACACATGTTAAGCAACCCAAAAATGGAAACATTTTCTTAAATTTTGTGCATTCAATTATTGAAAAGCAAAAAAGTTAAAGTCAAATTCATTAATTACAATAGAATATTTCTATTTTGAATCATTAACTTGTGCCTTGAGGGCACAAGTTAACATTTCCCATAGTTTAAATACAATTATATACTTGATTAATTACAGGTTGTGGAGTTGCAAGCAGAGTTAGCCTATGTACAAGCCCGTCTTTCAATATTGAATCGCATCTCTGAAGATCCTCAATTTCAACAGCCTCAAAGCTCTTCAATATCACCAACTTTGCATTCATCCTTACTTCATCATTTGTCATCAAGCAATGAAACTTCTTACATGTCCATGCACTTTGGTCATCCTCTTCAAAATGATCAATATCATCCAACACCTTTTGAAAATCCATCATACTATTCCTATAATCAACAACTTCATGACCAGGAATTCCAAGCTTTGCCTTGA
Protein sequence

>MS.gene016232.t1

MDLGCGDDGGGPCGACKFLRRKCVKGCCIFAPYFESSGQGSAHFAAVHKVFGASNAAKLLTRIPVHKRLDAVVTLCYEALARATDPVYGCVAHLFALQQQVVELQAELAYVQARLSILNRISEDPQFQQPQSSSISPTLHSSLLHHLSSSNETSYMSMHFGHPLQNDQYHPTPFENPSYYSYNQQLHDQEFQALP