AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016253

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016253.t1 MTR_7g074650 99.724 362 1 0 368 729 444 805 0.0 762
MS.gene016253.t1 MTR_7g074650 89.516 372 21 7 1 367 1 359 6.03e-168 503
MS.gene016253.t1 MTR_4g076020 59.322 354 127 4 368 717 90 430 1.96e-145 432
MS.gene016253.t1 MTR_1g069725 54.131 351 153 5 369 715 116 462 5.71e-127 386
MS.gene016253.t1 MTR_7g027190 41.129 372 192 6 368 716 306 673 3.06e-90 296
MS.gene016253.t1 MTR_1g029420 37.736 371 216 6 360 716 223 592 3.35e-65 227
MS.gene016253.t1 MTR_3g065980 36.340 377 202 11 368 718 178 542 1.05e-61 216
MS.gene016253.t1 MTR_4g102790 35.200 375 209 10 368 717 298 663 6.74e-61 217
MS.gene016253.t1 MTR_3g089055 35.580 371 224 6 360 716 201 570 9.98e-61 214
MS.gene016253.t1 MTR_3g089055 35.580 371 224 6 360 716 196 565 1.32e-60 214
MS.gene016253.t1 MTR_2g082090 37.389 337 196 6 393 715 243 578 2.72e-59 211
MS.gene016253.t1 MTR_5g094450 35.215 372 222 8 363 716 162 532 1.70e-57 204
MS.gene016253.t1 MTR_5g097480 34.153 366 220 7 368 716 183 544 3.50e-56 201
MS.gene016253.t1 MTR_3g056110 33.333 363 227 6 368 716 181 542 4.97e-54 195
MS.gene016253.t1 MTR_4g133660 35.608 337 202 6 393 715 207 542 1.19e-53 194
MS.gene016253.t1 MTR_8g442410 34.332 367 221 7 368 716 161 525 7.99e-53 192
MS.gene016253.t1 MTR_5g058860 35.904 376 208 12 371 715 123 496 1.43e-52 190
MS.gene016253.t1 MTR_4g077760 35.988 339 195 10 393 716 224 555 1.84e-52 191
MS.gene016253.t1 MTR_1g096030 32.546 381 216 12 369 716 87 459 1.58e-51 186
MS.gene016253.t1 MTR_3g072710 36.267 375 203 14 371 715 131 499 3.59e-50 184
MS.gene016253.t1 MTR_2g097410 31.421 366 235 6 368 717 377 742 4.99e-50 187
MS.gene016253.t1 MTR_3g022830 29.167 408 228 8 368 716 31 436 7.88e-50 181
MS.gene016253.t1 MTR_2g026250 30.256 390 251 8 341 716 214 596 1.37e-49 184
MS.gene016253.t1 MTR_7g062120 30.220 364 240 6 368 717 378 741 8.28e-48 181
MS.gene016253.t1 MTR_4g104020 31.250 368 232 8 368 716 155 520 8.70e-48 177
MS.gene016253.t1 MTR_4g064120 29.539 369 240 7 368 717 260 627 2.67e-46 175
MS.gene016253.t1 MTR_4g064160 30.495 364 239 5 368 717 322 685 6.74e-46 174
MS.gene016253.t1 MTR_4g064150 30.163 368 239 7 368 717 367 734 9.54e-46 175
MS.gene016253.t1 MTR_4g064200 30.328 366 235 8 368 717 290 651 1.11e-45 173
MS.gene016253.t1 MTR_4g064180 29.810 369 239 8 368 717 260 627 7.63e-45 171
MS.gene016253.t1 MTR_2g097310 31.436 369 227 10 368 717 278 639 1.14e-43 167
MS.gene016253.t1 MTR_2g097350 30.854 363 237 8 368 717 280 641 1.48e-43 167
MS.gene016253.t1 MTR_2g097467 28.846 364 242 6 368 717 295 655 1.52e-42 164
MS.gene016253.t1 MTR_2g097463 29.121 364 241 6 368 717 296 656 1.65e-41 161
MS.gene016253.t1 MTR_4g097080 28.378 370 239 11 369 717 140 504 1.89e-41 159
MS.gene016253.t1 MTR_2g097390 30.579 363 238 8 368 717 281 642 1.73e-40 158
MS.gene016253.t1 MTR_7g109580 31.165 369 239 10 361 716 188 554 2.08e-39 154
MS.gene016253.t1 MTR_2g097473 28.571 364 243 7 368 717 295 655 2.12e-39 155
MS.gene016253.t1 MTR_2g097380 30.495 364 208 11 368 717 230 562 2.57e-39 154
MS.gene016253.t1 MTR_2g034280 31.351 370 230 11 368 716 209 575 4.43e-39 153
MS.gene016253.t1 MTR_5g015490 28.191 376 238 13 369 717 121 491 1.45e-38 150
MS.gene016253.t1 MTR_5g009080 40.957 188 106 2 370 554 64 249 3.03e-38 149
MS.gene016253.t1 MTR_5g009080 38.255 149 87 3 570 716 333 478 1.07e-22 102
MS.gene016253.t1 MTR_2g034260 31.099 373 229 12 368 716 216 584 9.41e-38 149
MS.gene016253.t1 MTR_2g034250 30.108 372 235 12 368 716 216 585 1.93e-36 145
MS.gene016253.t1 MTR_4g076140 40.107 187 109 2 370 554 56 241 2.06e-35 140
MS.gene016253.t1 MTR_4g076140 37.584 149 88 3 570 716 324 469 2.61e-20 95.1
MS.gene016253.t1 MTR_2g099110 26.649 379 241 9 369 717 81 452 6.70e-35 139
MS.gene016253.t1 MTR_8g093070 28.698 338 206 12 398 717 177 497 2.21e-32 132
MS.gene016253.t1 MTR_7g057230 27.273 374 243 11 361 715 293 656 7.45e-27 116
MS.gene016253.t1 MTR_4g026485 26.178 382 251 8 352 716 257 624 7.92e-27 116
MS.gene016253.t1 MTR_0092s0100 27.520 367 242 8 363 716 373 728 1.48e-26 116
MS.gene016253.t1 MTR_0092s0100 27.520 367 242 8 363 716 376 731 1.58e-26 116
MS.gene016253.t1 MTR_1g086970 25.187 401 264 13 347 716 83 478 1.89e-26 114
MS.gene016253.t1 MTR_8g077940 27.500 360 232 13 374 716 194 541 4.53e-24 107
MS.gene016253.t1 MTR_6g047750 28.940 349 206 13 395 716 329 662 2.68e-22 102
MS.gene016253.t1 MTR_2g089100 21.939 392 257 11 369 717 59 444 6.77e-20 94.0
MS.gene016253.t1 MTR_5g015950 23.114 411 249 14 369 717 42 447 2.15e-18 89.0
MS.gene016253.t1 MTR_3g053270 23.114 411 249 14 369 717 42 447 2.15e-18 89.0
MS.gene016253.t1 MTR_8g020840 24.479 384 241 13 369 716 178 548 7.73e-18 88.2
MS.gene016253.t1 MTR_4g095500 23.415 410 247 14 369 717 66 469 2.69e-15 79.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016253.t1 AT3G54220 64.098 571 130 8 201 717 102 651 0.0 707
MS.gene016253.t1 AT5G41920 57.222 360 142 6 368 719 49 404 7.17e-137 409
MS.gene016253.t1 AT1G14920 37.534 365 199 11 368 716 178 529 7.22e-63 219
MS.gene016253.t1 AT1G66350 37.255 357 205 10 368 716 161 506 1.03e-62 218
MS.gene016253.t1 AT3G03450 37.500 368 200 11 368 716 189 545 1.22e-61 216
MS.gene016253.t1 AT1G63100 35.580 371 216 9 368 715 258 628 1.42e-60 215
MS.gene016253.t1 AT1G63100 35.580 371 216 9 368 715 283 653 3.98e-60 214
MS.gene016253.t1 AT1G50600 35.598 368 222 6 363 716 160 526 7.53e-60 211
MS.gene016253.t1 AT2G01570 36.986 365 201 11 368 716 230 581 1.13e-59 212
MS.gene016253.t1 AT1G50600 35.598 368 222 6 363 716 231 597 1.80e-59 211
MS.gene016253.t1 AT1G50600 35.598 368 222 6 363 716 235 601 1.97e-59 211
MS.gene016253.t1 AT2G04890 35.278 360 217 7 368 716 59 413 5.00e-59 206
MS.gene016253.t1 AT5G17490 34.406 404 236 12 331 716 124 516 5.68e-58 206
MS.gene016253.t1 AT5G66770 36.066 366 207 12 370 716 227 584 4.65e-55 199
MS.gene016253.t1 AT5G48150 33.693 371 216 9 368 716 128 490 7.98e-52 188
MS.gene016253.t1 AT5G48150 33.693 371 216 9 368 716 128 490 7.98e-52 188
MS.gene016253.t1 AT5G48150 33.693 371 216 9 368 716 128 490 7.98e-52 188
MS.gene016253.t1 AT5G48150 33.693 371 216 9 368 716 128 490 7.98e-52 188
MS.gene016253.t1 AT1G21450 34.711 363 221 5 369 716 232 593 8.73e-52 190
MS.gene016253.t1 AT4G17230 34.153 366 220 7 369 717 165 526 3.00e-51 187
MS.gene016253.t1 AT4G17230 34.153 366 220 7 369 717 165 526 3.00e-51 187
MS.gene016253.t1 AT5G59450 28.191 376 251 8 368 724 233 608 4.49e-46 174
MS.gene016253.t1 AT1G07530 30.959 365 237 6 368 717 402 766 2.31e-45 174
MS.gene016253.t1 AT2G37650 29.945 364 239 6 368 717 341 702 1.64e-44 171
MS.gene016253.t1 AT2G37650 29.945 364 239 6 368 717 353 714 1.69e-44 171
MS.gene016253.t1 AT3G50650 32.692 364 220 10 372 716 185 542 6.62e-44 166
MS.gene016253.t1 AT1G55580 32.576 396 216 12 370 716 52 445 1.19e-42 161
MS.gene016253.t1 AT2G29060 30.352 369 236 9 368 717 290 656 1.28e-40 159
MS.gene016253.t1 AT2G29060 30.352 369 236 9 368 717 327 693 1.41e-40 159
MS.gene016253.t1 AT3G46600 29.235 366 240 8 368 717 10 372 2.04e-40 153
MS.gene016253.t1 AT3G46600 29.235 366 240 8 368 717 218 580 8.85e-40 155
MS.gene016253.t1 AT3G46600 29.235 366 240 8 368 717 186 548 1.25e-39 154
MS.gene016253.t1 AT4G37650 27.822 381 240 10 369 717 153 530 1.11e-38 151
MS.gene016253.t1 AT4G08250 30.337 356 230 8 376 715 123 476 6.61e-38 148
MS.gene016253.t1 AT2G29065 28.000 375 242 10 368 717 258 629 4.95e-36 145
MS.gene016253.t1 AT1G07520 28.342 374 241 9 368 717 325 695 7.06e-36 145
MS.gene016253.t1 AT1G07520 28.342 374 241 9 368 717 324 694 7.54e-36 145
MS.gene016253.t1 AT1G07520 28.342 374 241 9 368 717 160 530 1.00e-35 142
MS.gene016253.t1 AT1G07520 28.342 374 241 9 368 717 160 530 1.00e-35 142
MS.gene016253.t1 AT1G50420 38.830 188 112 1 370 554 65 252 2.12e-34 137
MS.gene016253.t1 AT1G50420 34.416 154 96 3 565 716 329 479 2.96e-19 92.0
MS.gene016253.t1 AT5G52510 28.571 378 228 11 369 716 275 640 2.63e-30 127
MS.gene016253.t1 AT4G36710 26.776 366 240 11 368 716 131 485 7.13e-25 109
MS.gene016253.t1 AT3G60630 30.351 313 170 11 434 716 328 622 8.69e-25 110
MS.gene016253.t1 AT4G00150 28.863 343 210 13 387 716 233 554 1.78e-23 105
MS.gene016253.t1 AT2G45160 29.508 305 180 10 434 716 348 639 6.70e-22 101
MS.gene016253.t1 AT3G49950 25.858 379 245 15 369 717 37 409 6.30e-19 90.5
MS.gene016253.t1 AT3G13840 25.991 454 279 18 298 716 79 510 1.17e-18 90.5

Find 111 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCCAACCAAGTTTA+AGG 0.116743 7.1:-28302175 None:intergenic
ATCAAAACATGTTTCCTAAT+TGG 0.117353 7.1:+28300325 MS.gene016253:CDS
TTACCTTCTTCCACAGAAAA+AGG 0.239785 7.1:+28299535 MS.gene016253:CDS
TCAAAACATGTTTCCTAATT+GGG 0.250183 7.1:+28300326 MS.gene016253:CDS
ACAGTTACTGTTTCTGTTGC+TGG 0.272831 7.1:-28299905 None:intergenic
TCCACTACTGTTACCACTTT+AGG 0.280461 7.1:-28301816 None:intergenic
TATACTTTGGTTGAGGATAA+TGG 0.311713 7.1:+28302149 MS.gene016253:CDS
ATTTGTGGCACACCCCAATT+AGG 0.312254 7.1:-28300339 None:intergenic
GCTGCTTGTGCTTTGTTCAA+TGG 0.326297 7.1:+28299250 MS.gene016253:CDS
CATTCTCTATATGATGTTAC+TGG 0.333927 7.1:+28301173 MS.gene016253:CDS
GAGCAATGCAGGTTCATTCT+TGG 0.338861 7.1:+28301848 MS.gene016253:CDS
TCGCAGCTTTCGACGCCTTT+CGG 0.348784 7.1:+28300654 MS.gene016253:CDS
TTTCCAACTTTCTCAGCAAC+AGG 0.359463 7.1:-28301089 None:intergenic
TCAGCATTATTTGATTCTCT+TGG 0.361167 7.1:+28301900 MS.gene016253:CDS
TTTATCTCACCACTCCTTGA+TGG 0.363245 7.1:-28302014 None:intergenic
ACACAAGCTAGTCTTCTTCT+TGG 0.369111 7.1:+28302107 MS.gene016253:CDS
TGTTGAAAATGAGTGTAGTT+TGG 0.371295 7.1:-28299793 None:intergenic
AACCATGTTCCTAATATTTG+TGG 0.381285 7.1:+28299835 MS.gene016253:CDS
GTTTCTGTTGCTGGTTCTGT+TGG 0.383646 7.1:-28299896 None:intergenic
AAACCTCAGCTGCAACAAAC+TGG 0.395103 7.1:+28299989 MS.gene016253:CDS
TATGTGAGACTCACTGGATT+AGG 0.397735 7.1:+28300984 MS.gene016253:CDS
TAACTCTTCACCTTCTATGA+TGG 0.414564 7.1:+28299963 MS.gene016253:CDS
GTGAGATAAAGTTTCACAAT+TGG 0.417914 7.1:+28302027 MS.gene016253:CDS
CAGAAGCTGTTGCTGTTCAT+TGG 0.419326 7.1:+28301144 MS.gene016253:CDS
TTCATTCTTGGGAAGGTTTG+TGG 0.421301 7.1:+28301860 MS.gene016253:CDS
TCAAGAACAAGAGCAAGGTT+TGG 0.421529 7.1:-28300108 None:intergenic
CAAAAGCTTCTTGTATAGCT+TGG 0.424689 7.1:-28300860 None:intergenic
GCTGCAACAAACTGGATTGA+TGG 0.429027 7.1:+28299997 MS.gene016253:CDS
ATGGTATCCTTAAACTTGGT+TGG 0.429657 7.1:+28302168 MS.gene016253:CDS
AGCAATGCAGGTTCATTCTT+GGG 0.429990 7.1:+28301849 MS.gene016253:CDS
CCCTTCTGAAGGGTATACTT+TGG 0.433004 7.1:+28302136 MS.gene016253:CDS
ATCTTCACTACTGATATTGC+TGG 0.434098 7.1:-28299314 None:intergenic
GATTCTCTTGGTTCAAGCTA+TGG 0.434363 7.1:+28301912 MS.gene016253:CDS
TGTTGCTGGTTCTGTTGGGT+TGG 0.440162 7.1:-28299891 None:intergenic
TTTCCTGTTGCTGAGAAAGT+TGG 0.444010 7.1:+28301086 MS.gene016253:CDS
CATATCCTTGCTTCAAGACC+AGG 0.445706 7.1:+28300951 MS.gene016253:CDS
TTTCTGTTGCTGGTTCTGTT+GGG 0.451663 7.1:-28299895 None:intergenic
CTTGGCATGTTCCCTTCTGA+AGG 0.451744 7.1:+28302125 MS.gene016253:CDS
GTTTGCTTACTGCTTCTGCT+TGG 0.455432 7.1:+28302201 MS.gene016253:CDS
TTGCTCTTGTTCTTGAACAT+AGG 0.455705 7.1:+28300115 MS.gene016253:CDS
TTGAGATTGTTGGAAGAAGA+AGG 0.456597 7.1:-28299748 None:intergenic
ATGAGTGTAGTTTGGAGCAT+TGG 0.456908 7.1:-28299785 None:intergenic
GTTTGGAGCATTGGAGTTTG+AGG 0.456950 7.1:-28299776 None:intergenic
CGTAACGTTCTTGCAGTTGG+TGG 0.465242 7.1:+28301990 MS.gene016253:CDS
TTTAGAGGTATCTCTTTGGC+TGG 0.469891 7.1:+28302074 MS.gene016253:CDS
GTTTGAAGCACTATTGTTGT+TGG 0.471893 7.1:-28299290 None:intergenic
CCTCCTTATGTGAGACTCAC+TGG 0.475283 7.1:+28300978 MS.gene016253:CDS
TGAGGAATTGTTGAGATTGT+TGG 0.475428 7.1:-28299758 None:intergenic
GCAACAGGAAAGAACTCAAA+AGG 0.485861 7.1:-28301074 None:intergenic
ATTGTTGATAAGTTGAGGAA+TGG 0.488023 7.1:-28300058 None:intergenic
GAACAAGACTTGAGCAATGC+AGG 0.491335 7.1:+28301837 MS.gene016253:CDS
TTTCTCTCAGGAACAACACT+TGG 0.491378 7.1:-28300162 None:intergenic
GTTCAGATACAAACACTTTG+TGG 0.494320 7.1:+28301195 MS.gene016253:CDS
AAATGAGAGAATTTCACAAA+TGG 0.504284 7.1:-28300828 None:intergenic
TGAAGGGTATACTTTGGTTG+AGG 0.506995 7.1:+28302142 MS.gene016253:CDS
TAGCAACATAGTGGAGTAGT+TGG 0.507219 7.1:-28299725 None:intergenic
TATGACATTGTTGGTATGAA+AGG 0.510105 7.1:-28302231 None:intergenic
AATCCAGTTTGTTGCAGCTG+AGG 0.510974 7.1:-28299992 None:intergenic
TTGTTGTTGTTGTGGCATGT+TGG 0.511794 7.1:-28299362 None:intergenic
AAAATCTATTGTTGTTGTTG+TGG 0.516540 7.1:-28299459 None:intergenic
CACTGGATTAGGTACTTCCA+TGG 0.517366 7.1:+28300995 MS.gene016253:CDS
ATGGAAACACTTGAAGCTAC+TGG 0.517893 7.1:+28301014 MS.gene016253:CDS
CTCACATAAGGAGGTCCACC+TGG 0.520829 7.1:-28300969 None:intergenic
AGTAGCTTCAAGTGTTTCCA+TGG 0.526271 7.1:-28301012 None:intergenic
CAAAACATGTTTCCTAATTG+GGG 0.527048 7.1:+28300327 MS.gene016253:CDS
ATGCAAGGTTTACAATGGCC+TGG 0.527385 7.1:+28300921 MS.gene016253:CDS
GATAATGGTATCCTTAAACT+TGG 0.528102 7.1:+28302164 MS.gene016253:CDS
ATTCGTAACGTTCTTGCAGT+TGG 0.528879 7.1:+28301987 MS.gene016253:CDS
ACACTTTGTGGCTCTTGCAA+AGG 0.531826 7.1:+28301207 MS.gene016253:CDS
TTGGCATGTTCCCTTCTGAA+GGG 0.532685 7.1:+28302126 MS.gene016253:CDS
AGTAACTGTAACATAAGTTC+TGG 0.539385 7.1:+28299919 MS.gene016253:CDS
CTAACATTGTTGATAAGTTG+AGG 0.539907 7.1:-28300063 None:intergenic
AGAGCAAGGTTTGGATTACA+AGG 0.541984 7.1:-28300099 None:intergenic
ACCAAAGTATACCCTTCAGA+AGG 0.543263 7.1:-28302137 None:intergenic
TTGGTTGTGACGGTCACAGT+GGG 0.545379 7.1:-28299706 None:intergenic
TTGCAGTTGGTGGACCATCA+AGG 0.546702 7.1:+28302000 MS.gene016253:CDS
ATGCAGGTTCATTCTTGGGA+AGG 0.550894 7.1:+28301853 MS.gene016253:CDS
GAGGTTTCATCCATCATAGA+AGG 0.554125 7.1:-28299973 None:intergenic
TCATACCACATCATAACTGA+TGG 0.555692 7.1:+28302249 MS.gene016253:CDS
GAGGAATTAACATTAGAAGC+AGG 0.557000 7.1:-28300231 None:intergenic
ATGACATTGTTGGTATGAAA+GGG 0.560826 7.1:-28302230 None:intergenic
AGAAGAAGGTAGCAACATAG+TGG 0.560888 7.1:-28299734 None:intergenic
ATGTAGGTTGGCTCCTAAAG+TGG 0.561512 7.1:+28301803 MS.gene016253:intron
AATCCAGTGAGTCTCACATA+AGG 0.568715 7.1:-28300981 None:intergenic
ATTATTATTATTTGTACCTG+CGG 0.572319 7.1:-28299581 None:intergenic
ATAGATCTTGATATCATGCA+AGG 0.576982 7.1:+28300906 MS.gene016253:CDS
AGAGAGTGAAGAGAGACATG+TGG 0.579883 7.1:+28301938 MS.gene016253:CDS
GTCCACCTGGTCTTGAAGCA+AGG 0.584919 7.1:-28300956 None:intergenic
ATGATGTGGTATGACATTGT+TGG 0.587203 7.1:-28302240 None:intergenic
ATATCATGCAAGGTTTACAA+TGG 0.601849 7.1:+28300916 MS.gene016253:CDS
AGACTAGTGAGTTCATGTCT+AGG 0.606766 7.1:+28300726 MS.gene016253:CDS
AACCACAAATATTAGGAACA+TGG 0.615138 7.1:-28299837 None:intergenic
CTTTGTGCTGATGTGCCGAA+AGG 0.618512 7.1:-28300669 None:intergenic
TATGTTCAAGAACAAGAGCA+AGG 0.620046 7.1:-28300113 None:intergenic
GAGTGAAGAGAGACATGTGG+TGG 0.624909 7.1:+28301941 MS.gene016253:CDS
TCCTAAAGTGGTAACAGTAG+TGG 0.625925 7.1:+28301815 MS.gene016253:CDS
CCAAAGTATACCCTTCAGAA+GGG 0.626039 7.1:-28302136 None:intergenic
AGTGGAGTAGTTGGTTGTGA+CGG 0.635058 7.1:-28299716 None:intergenic
TAGATCCATCAGTTATGATG+TGG 0.639240 7.1:-28302254 None:intergenic
CCAGTGAGTCTCACATAAGG+AGG 0.642698 7.1:-28300978 None:intergenic
ACAACAACAACACTAGCCGC+AGG 0.647867 7.1:+28299565 MS.gene016253:CDS
ACATTGTTGGTATGAAAGGG+AGG 0.648110 7.1:-28302227 None:intergenic
TGATTGTGTAGTGTATGTGG+TGG 0.648692 7.1:-28300765 None:intergenic
GTTGGTTGTGACGGTCACAG+TGG 0.651008 7.1:-28299707 None:intergenic
ATCCTTGCTTCAAGACCAGG+TGG 0.657302 7.1:+28300954 MS.gene016253:CDS
AGAGAAAAGCTTCAACAGTG+TGG 0.663978 7.1:+28302050 MS.gene016253:CDS
AGGTAAAGTACAATTCAATG+AGG 0.676877 7.1:-28299518 None:intergenic
GTTGGTGGACCATCAAGGAG+TGG 0.725373 7.1:+28302005 MS.gene016253:CDS
GAGGAACAATGTCATCAACA+CGG 0.736939 7.1:-28300269 None:intergenic
TGGTTGTGACGGTCACAGTG+GGG 0.753890 7.1:-28299705 None:intergenic
ACGGTTCATGAGTTTCACTG+AGG 0.765117 7.1:-28300250 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAAAAAAAGCATAAAGTTT+TGG - chr7.1:28301395-28301414 None:intergenic 15.0%
!!! AAAAAAAAGCATAAAGTTTT+GGG - chr7.1:28301394-28301413 None:intergenic 15.0%
!! ATATAATCTACATGATTTAG+TGG + chr7.1:28301328-28301347 MS.gene016253:intron 20.0%
!! ATTATTATTATTTGTACCTG+CGG - chr7.1:28299584-28299603 None:intergenic 20.0%
!! GGTAATAGTAATAAAAGTTT+AGG + chr7.1:28301232-28301251 MS.gene016253:intron 20.0%
!! TATAGTTTCTAATTATCCAT+TGG + chr7.1:28301623-28301642 MS.gene016253:intron 20.0%
!! TTCAAAAATACACAAATAAG+AGG + chr7.1:28301724-28301743 MS.gene016253:intron 20.0%
!! TTTATCTGTGTTATATATGT+AGG + chr7.1:28301787-28301806 MS.gene016253:intron 20.0%
!!! CGAAATTTTGATTTCAAATA+CGG - chr7.1:28301753-28301772 None:intergenic 20.0%
!!! TTATTGTTGTTATTGATTTG+TGG - chr7.1:28300357-28300376 None:intergenic 20.0%
! AAATGAGAGAATTTCACAAA+TGG - chr7.1:28300831-28300850 None:intergenic 25.0%
! ATCAAAACATGTTTCCTAAT+TGG + chr7.1:28300325-28300344 MS.gene016253:CDS 25.0%
! ATTGAAGAAGTAATGTTAGA+AGG - chr7.1:28300575-28300594 None:intergenic 25.0%
! CAAACTATAACATCATTTCA+TGG - chr7.1:28301270-28301289 None:intergenic 25.0%
! TATACAAAACCAACAAGAAT+TGG - chr7.1:28301309-28301328 None:intergenic 25.0%
! TCAAAACATGTTTCCTAATT+GGG + chr7.1:28300326-28300345 MS.gene016253:CDS 25.0%
!! AAAATCTATTGTTGTTGTTG+TGG - chr7.1:28299462-28299481 None:intergenic 25.0%
!! TTACCTTTAGAAGAGAAAAA+AGG - chr7.1:28301452-28301471 None:intergenic 25.0%
!! TTCTCTTCTAAAGGTAAAAT+TGG + chr7.1:28301455-28301474 MS.gene016253:intron 25.0%
!!! AAAAGTGCTTAGAAAAAGAA+TGG + chr7.1:28299408-28299427 MS.gene016253:CDS 25.0%
!!! TTTGCATGGGATTTTTATTT+TGG + chr7.1:28301647-28301666 MS.gene016253:intron 25.0%
AACCACAAATATTAGGAACA+TGG - chr7.1:28299840-28299859 None:intergenic 30.0%
AACCATGTTCCTAATATTTG+TGG + chr7.1:28299835-28299854 MS.gene016253:CDS 30.0%
AATTATCCATTGGATTTGCA+TGG + chr7.1:28301633-28301652 MS.gene016253:intron 30.0%
AGGTAAAGTACAATTCAATG+AGG - chr7.1:28299521-28299540 None:intergenic 30.0%
AGTAACAAGAAAGAAGAAAG+AGG + chr7.1:28300509-28300528 MS.gene016253:intron 30.0%
AGTAACTGTAACATAAGTTC+TGG + chr7.1:28299919-28299938 MS.gene016253:CDS 30.0%
ATAGATCTTGATATCATGCA+AGG + chr7.1:28300906-28300925 MS.gene016253:CDS 30.0%
ATATCATGCAAGGTTTACAA+TGG + chr7.1:28300916-28300935 MS.gene016253:CDS 30.0%
ATGACATTGTTGGTATGAAA+GGG - chr7.1:28302233-28302252 None:intergenic 30.0%
ATTATCCATTGGATTTGCAT+GGG + chr7.1:28301634-28301653 MS.gene016253:intron 30.0%
CAAAACATGTTTCCTAATTG+GGG + chr7.1:28300327-28300346 MS.gene016253:CDS 30.0%
CAAAAGAAGAAAGATGAAGA+AGG + chr7.1:28300543-28300562 MS.gene016253:intron 30.0%
CAATAACAACAATAACCCTT+TGG + chr7.1:28300362-28300381 MS.gene016253:intron 30.0%
CATTCTCTATATGATGTTAC+TGG + chr7.1:28301173-28301192 MS.gene016253:CDS 30.0%
CCAGAAAAACCACAAATATT+AGG - chr7.1:28299847-28299866 None:intergenic 30.0%
CTTGATGAATCAGAAAAATG+AGG - chr7.1:28300291-28300310 None:intergenic 30.0%
GTGAGATAAAGTTTCACAAT+TGG + chr7.1:28302027-28302046 MS.gene016253:CDS 30.0%
TATACTTTGGTTGAGGATAA+TGG + chr7.1:28302149-28302168 MS.gene016253:CDS 30.0%
TATGACATTGTTGGTATGAA+AGG - chr7.1:28302234-28302253 None:intergenic 30.0%
TCAGCATTATTTGATTCTCT+TGG + chr7.1:28301900-28301919 MS.gene016253:CDS 30.0%
TCTGTGTTATATATGTAGGT+TGG + chr7.1:28301791-28301810 MS.gene016253:intron 30.0%
TGTTGAAAATGAGTGTAGTT+TGG - chr7.1:28299796-28299815 None:intergenic 30.0%
! AAAAAGAATGGCTTCTGAAA+TGG + chr7.1:28299420-28299439 MS.gene016253:CDS 30.0%
! ATTGTTGATAAGTTGAGGAA+TGG - chr7.1:28300061-28300080 None:intergenic 30.0%
! CCTAATATTTGTGGTTTTTC+TGG + chr7.1:28299844-28299863 MS.gene016253:CDS 30.0%
! CTAACATTGTTGATAAGTTG+AGG - chr7.1:28300066-28300085 None:intergenic 30.0%
! GATAATGGTATCCTTAAACT+TGG + chr7.1:28302164-28302183 MS.gene016253:CDS 30.0%
! TACTCTTTTCCAATTCTTGT+TGG + chr7.1:28301297-28301316 MS.gene016253:intron 30.0%
! TCTGATTTTGCATCTAAACT+TGG + chr7.1:28301047-28301066 MS.gene016253:CDS 30.0%
!! TTTTGATTGTGTAGTGTATG+TGG - chr7.1:28300771-28300790 None:intergenic 30.0%
!!! CTACCTTTTTTCTCTTCTAA+AGG + chr7.1:28301446-28301465 MS.gene016253:intron 30.0%
AAAATCCCATGCAAATCCAA+TGG - chr7.1:28301642-28301661 None:intergenic 35.0%
ATCTTCACTACTGATATTGC+TGG - chr7.1:28299317-28299336 None:intergenic 35.0%
ATGATGTGGTATGACATTGT+TGG - chr7.1:28302243-28302262 None:intergenic 35.0%
ATGGTATCCTTAAACTTGGT+TGG + chr7.1:28302168-28302187 MS.gene016253:CDS 35.0%
CAAAAGCTTCTTGTATAGCT+TGG - chr7.1:28300863-28300882 None:intergenic 35.0%
GAGGAATTAACATTAGAAGC+AGG - chr7.1:28300234-28300253 None:intergenic 35.0%
GATCTTTCCAACCAAGTTTA+AGG - chr7.1:28302178-28302197 None:intergenic 35.0%
GTGAAGGTAAAGTAACCAAA+GGG - chr7.1:28300380-28300399 None:intergenic 35.0%
GTGAGCACTATAGTCTATAA+AGG - chr7.1:28301419-28301438 None:intergenic 35.0%
GTTCAGATACAAACACTTTG+TGG + chr7.1:28301195-28301214 MS.gene016253:CDS 35.0%
TAACTCTTCACCTTCTATGA+TGG + chr7.1:28299963-28299982 MS.gene016253:CDS 35.0%
TATGTTCAAGAACAAGAGCA+AGG - chr7.1:28300116-28300135 None:intergenic 35.0%
TGAGGAATTGTTGAGATTGT+TGG - chr7.1:28299761-28299780 None:intergenic 35.0%
TGATTTAGTGGTTGCTGATA+TGG + chr7.1:28301340-28301359 MS.gene016253:intron 35.0%
TGTGAAGGTAAAGTAACCAA+AGG - chr7.1:28300381-28300400 None:intergenic 35.0%
TTACCTTCTTCCACAGAAAA+AGG + chr7.1:28299535-28299554 MS.gene016253:CDS 35.0%
TTGAGATTGTTGGAAGAAGA+AGG - chr7.1:28299751-28299770 None:intergenic 35.0%
TTGCTCTTGTTCTTGAACAT+AGG + chr7.1:28300115-28300134 MS.gene016253:CDS 35.0%
! AGCACTTTTCTTTCAGAATG+AGG - chr7.1:28299397-28299416 None:intergenic 35.0%
! GTTTGAAGCACTATTGTTGT+TGG - chr7.1:28299293-28299312 None:intergenic 35.0%
! TGTTGTAACACCTTTTTCTG+TGG - chr7.1:28299548-28299567 None:intergenic 35.0%
! TGTTGTTGTTGTTGTTGTTG+TGG - chr7.1:28299373-28299392 None:intergenic 35.0%
!! CTGGATTGATGGTATTTTGA+AGG + chr7.1:28300008-28300027 MS.gene016253:CDS 35.0%
!! TGATCTTGTTGTGTTGATTG+TGG - chr7.1:28300405-28300424 None:intergenic 35.0%
!! TTTTCAACAACCACTTGTTG+AGG + chr7.1:28299807-28299826 MS.gene016253:CDS 35.0%
!!! GTGTTGTTTCTTTTTCTCTC+AGG - chr7.1:28300177-28300196 None:intergenic 35.0%
!!! TCAGCTTTTCAAGTCTTCAA+TGG + chr7.1:28300798-28300817 MS.gene016253:CDS 35.0%
!!! TGGTTTTAGAGGTATCTCTT+TGG + chr7.1:28302070-28302089 MS.gene016253:CDS 35.0%
!!! TGGTTTTGAGATGGAAAAAG+TGG - chr7.1:28299874-28299893 None:intergenic 35.0%
AATCCAGTGAGTCTCACATA+AGG - chr7.1:28300984-28301003 None:intergenic 40.0%
ACATTGTTGGTATGAAAGGG+AGG - chr7.1:28302230-28302249 None:intergenic 40.0%
ACCAAAGTATACCCTTCAGA+AGG - chr7.1:28302140-28302159 None:intergenic 40.0%
AGAAGAAGGTAGCAACATAG+TGG - chr7.1:28299737-28299756 None:intergenic 40.0%
AGACTAGTGAGTTCATGTCT+AGG + chr7.1:28300726-28300745 MS.gene016253:CDS 40.0%
AGAGAAAAGCTTCAACAGTG+TGG + chr7.1:28302050-28302069 MS.gene016253:CDS 40.0%
AGAGCAAGGTTTGGATTACA+AGG - chr7.1:28300102-28300121 None:intergenic 40.0%
AGCAATGCAGGTTCATTCTT+GGG + chr7.1:28301849-28301868 MS.gene016253:CDS 40.0%
AGTAGCTTCAAGTGTTTCCA+TGG - chr7.1:28301015-28301034 None:intergenic 40.0%
ATGGAAACACTTGAAGCTAC+TGG + chr7.1:28301014-28301033 MS.gene016253:CDS 40.0%
ATTCGTAACGTTCTTGCAGT+TGG + chr7.1:28301987-28302006 MS.gene016253:CDS 40.0%
CCAAAGTATACCCTTCAGAA+GGG - chr7.1:28302139-28302158 None:intergenic 40.0%
GAGGAACAATGTCATCAACA+CGG - chr7.1:28300272-28300291 None:intergenic 40.0%
GAGGTTTCATCCATCATAGA+AGG - chr7.1:28299976-28299995 None:intergenic 40.0%
GATTCTCTTGGTTCAAGCTA+TGG + chr7.1:28301912-28301931 MS.gene016253:CDS 40.0%
GCAACAGGAAAGAACTCAAA+AGG - chr7.1:28301077-28301096 None:intergenic 40.0%
GCAAGGATATGAAAAAGACC+AGG - chr7.1:28300942-28300961 None:intergenic 40.0%
TATGTGAGACTCACTGGATT+AGG + chr7.1:28300984-28301003 MS.gene016253:CDS 40.0%
TCAAGAACAAGAGCAAGGTT+TGG - chr7.1:28300111-28300130 None:intergenic 40.0%
TCCACTACTGTTACCACTTT+AGG - chr7.1:28301819-28301838 None:intergenic 40.0%
TCCTAAAGTGGTAACAGTAG+TGG + chr7.1:28301815-28301834 MS.gene016253:CDS 40.0%
TGATTGTGTAGTGTATGTGG+TGG - chr7.1:28300768-28300787 None:intergenic 40.0%
TTCATTCTTGGGAAGGTTTG+TGG + chr7.1:28301860-28301879 MS.gene016253:CDS 40.0%
TTTATCTCACCACTCCTTGA+TGG - chr7.1:28302017-28302036 None:intergenic 40.0%
TTTCCAACTTTCTCAGCAAC+AGG - chr7.1:28301092-28301111 None:intergenic 40.0%
TTTCCTGTTGCTGAGAAAGT+TGG + chr7.1:28301086-28301105 MS.gene016253:CDS 40.0%
TTTCTCTCAGGAACAACACT+TGG - chr7.1:28300165-28300184 None:intergenic 40.0%
! ACACCTTTTTCTGTGGAAGA+AGG - chr7.1:28299541-28299560 None:intergenic 40.0%
! ATGAGTGTAGTTTGGAGCAT+TGG - chr7.1:28299788-28299807 None:intergenic 40.0%
! TAGCAACATAGTGGAGTAGT+TGG - chr7.1:28299728-28299747 None:intergenic 40.0%
! TGAAGGGTATACTTTGGTTG+AGG + chr7.1:28302142-28302161 MS.gene016253:CDS 40.0%
! TTGTTGTTGTTGTGGCATGT+TGG - chr7.1:28299365-28299384 None:intergenic 40.0%
!! ACACAAGCTAGTCTTCTTCT+TGG + chr7.1:28302107-28302126 MS.gene016253:CDS 40.0%
!! ACAGTTACTGTTTCTGTTGC+TGG - chr7.1:28299908-28299927 None:intergenic 40.0%
!! TGTGTTGATTGTGGTTGTGA+AGG - chr7.1:28300396-28300415 None:intergenic 40.0%
!! TTTAGAGGTATCTCTTTGGC+TGG + chr7.1:28302074-28302093 MS.gene016253:CDS 40.0%
!! TTTCTGTTGCTGGTTCTGTT+GGG - chr7.1:28299898-28299917 None:intergenic 40.0%
!!! CTTCAACAGTGTGGTTTTAG+AGG + chr7.1:28302059-28302078 MS.gene016253:CDS 40.0%
!!! TGGTTTTGTTCCTCAACAAG+TGG - chr7.1:28299820-28299839 None:intergenic 40.0%
AAACCTCAGCTGCAACAAAC+TGG + chr7.1:28299989-28300008 MS.gene016253:CDS 45.0%
ACACTTTGTGGCTCTTGCAA+AGG + chr7.1:28301207-28301226 MS.gene016253:CDS 45.0%
ACGGTTCATGAGTTTCACTG+AGG - chr7.1:28300253-28300272 None:intergenic 45.0%
AGAGAGTGAAGAGAGACATG+TGG + chr7.1:28301938-28301957 MS.gene016253:CDS 45.0%
AGTGGAGTAGTTGGTTGTGA+CGG - chr7.1:28299719-28299738 None:intergenic 45.0%
ATGCAAGGTTTACAATGGCC+TGG + chr7.1:28300921-28300940 MS.gene016253:CDS 45.0%
ATGCAGGTTCATTCTTGGGA+AGG + chr7.1:28301853-28301872 MS.gene016253:CDS 45.0%
ATTTGTGGCACACCCCAATT+AGG - chr7.1:28300342-28300361 None:intergenic 45.0%
CATATCCTTGCTTCAAGACC+AGG + chr7.1:28300951-28300970 MS.gene016253:CDS 45.0%
GAACAAGACTTGAGCAATGC+AGG + chr7.1:28301837-28301856 MS.gene016253:CDS 45.0%
GAGCAATGCAGGTTCATTCT+TGG + chr7.1:28301848-28301867 MS.gene016253:CDS 45.0%
GCTGCAACAAACTGGATTGA+TGG + chr7.1:28299997-28300016 MS.gene016253:CDS 45.0%
GTTTGCTTACTGCTTCTGCT+TGG + chr7.1:28302201-28302220 MS.gene016253:CDS 45.0%
TTGGCATGTTCCCTTCTGAA+GGG + chr7.1:28302126-28302145 MS.gene016253:CDS 45.0%
TTTGTGGCTCTTGCAAAGGT+TGG + chr7.1:28301211-28301230 MS.gene016253:intron 45.0%
! ATGTAGGTTGGCTCCTAAAG+TGG + chr7.1:28301803-28301822 MS.gene016253:intron 45.0%
! CACTGGATTAGGTACTTCCA+TGG + chr7.1:28300995-28301014 MS.gene016253:CDS 45.0%
! CAGAAGCTGTTGCTGTTCAT+TGG + chr7.1:28301144-28301163 MS.gene016253:CDS 45.0%
! CCCTTCTGAAGGGTATACTT+TGG + chr7.1:28302136-28302155 MS.gene016253:CDS 45.0%
! GCTGCTTGTGCTTTGTTCAA+TGG + chr7.1:28299250-28299269 MS.gene016253:CDS 45.0%
!! AATCCAGTTTGTTGCAGCTG+AGG - chr7.1:28299995-28300014 None:intergenic 45.0%
!! GTTTCTGTTGCTGGTTCTGT+TGG - chr7.1:28299899-28299918 None:intergenic 45.0%
!! GTTTGGAGCATTGGAGTTTG+AGG - chr7.1:28299779-28299798 None:intergenic 45.0%
!!! CTGTTGGGTTGGTTTTGAGA+TGG - chr7.1:28299883-28299902 None:intergenic 45.0%
ACAACAACAACACTAGCCGC+AGG + chr7.1:28299565-28299584 MS.gene016253:CDS 50.0%
ACCACCAACTTCTGCTGAGT+TGG + chr7.1:28300485-28300504 MS.gene016253:intron 50.0%
ACTGCCAACTCAGCAGAAGT+TGG - chr7.1:28300492-28300511 None:intergenic 50.0%
ATCCTTGCTTCAAGACCAGG+TGG + chr7.1:28300954-28300973 MS.gene016253:CDS 50.0%
CCAGTGAGTCTCACATAAGG+AGG - chr7.1:28300981-28301000 None:intergenic 50.0%
CCTCCTTATGTGAGACTCAC+TGG + chr7.1:28300978-28300997 MS.gene016253:CDS 50.0%
CGTAACGTTCTTGCAGTTGG+TGG + chr7.1:28301990-28302009 MS.gene016253:CDS 50.0%
CTTGGCATGTTCCCTTCTGA+AGG + chr7.1:28302125-28302144 MS.gene016253:CDS 50.0%
GAGTGAAGAGAGACATGTGG+TGG + chr7.1:28301941-28301960 MS.gene016253:CDS 50.0%
TTGGTTGTGACGGTCACAGT+GGG - chr7.1:28299709-28299728 None:intergenic 50.0%
! CTTTGTGCTGATGTGCCGAA+AGG - chr7.1:28300672-28300691 None:intergenic 50.0%
!! AACTCAGCAGAAGTTGGTGG+TGG - chr7.1:28300486-28300505 None:intergenic 50.0%
!! TGTTGCTGGTTCTGTTGGGT+TGG - chr7.1:28299894-28299913 None:intergenic 50.0%
!! TTGCAGTTGGTGGACCATCA+AGG + chr7.1:28302000-28302019 MS.gene016253:CDS 50.0%
CTCACATAAGGAGGTCCACC+TGG - chr7.1:28300972-28300991 None:intergenic 55.0%
GCCAACTCAGCAGAAGTTGG+TGG - chr7.1:28300489-28300508 None:intergenic 55.0%
GTTGGTTGTGACGGTCACAG+TGG - chr7.1:28299710-28299729 None:intergenic 55.0%
TCGCAGCTTTCGACGCCTTT+CGG + chr7.1:28300654-28300673 MS.gene016253:CDS 55.0%
TGGTTGTGACGGTCACAGTG+GGG - chr7.1:28299708-28299727 None:intergenic 55.0%
! GTTGGTGGACCATCAAGGAG+TGG + chr7.1:28302005-28302024 MS.gene016253:CDS 55.0%
!! GTCCACCTGGTCTTGAAGCA+AGG - chr7.1:28300959-28300978 None:intergenic 55.0%
!! TCAGCAGAAGTTGGTGGTGG+TGG - chr7.1:28300483-28300502 None:intergenic 55.0%
!! GGTGGTGGTGGTGTTGTTGC+AGG - chr7.1:28300471-28300490 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 28299247 28302268 28299247 ID=MS.gene016253
chr7.1 mRNA 28299247 28302268 28299247 ID=MS.gene016253.t1;Parent=MS.gene016253
chr7.1 exon 28299247 28300348 28299247 ID=MS.gene016253.t1.exon1;Parent=MS.gene016253.t1
chr7.1 CDS 28299247 28300348 28299247 ID=cds.MS.gene016253.t1;Parent=MS.gene016253.t1
chr7.1 exon 28300601 28301228 28300601 ID=MS.gene016253.t1.exon2;Parent=MS.gene016253.t1
chr7.1 CDS 28300601 28301228 28300601 ID=cds.MS.gene016253.t1;Parent=MS.gene016253.t1
chr7.1 exon 28301809 28302268 28301809 ID=MS.gene016253.t1.exon3;Parent=MS.gene016253.t1
chr7.1 CDS 28301809 28302268 28301809 ID=cds.MS.gene016253.t1;Parent=MS.gene016253.t1
Gene Sequence

>MS.gene016253

ATGGCTGCTTGTGCTTTGTTCAATGGTGCTACTACTGATGAAACCAACAACAATAGTGCTTCAAACTCCAGCAATATCAGTAGTGAAGATTTACACACTCTACACAACAACAACACCAACATGCCACAACAACAACAACAACAACAACCTCATTCTGAAAGAAAAGTGCTTAGAAAAAGAATGGCTTCTGAAATGGAACTTCAACTTCAAGTCCACAACAACAACAATAGATTTTCTCGTAGAAACAACAACAACAGCAGCAACAACATTTCCTCATTGAATTGTACTTTACCTTCTTCCACAGAAAAAGGTGTTACAACAACAACAACACTAGCCGCAGGTACAAATAATAATAATAATAATTTTCATTATAATACTAATAATATAAACAATAGTAATAATATTAGTCATCATAGTAACGTTGCTTTGTCACGAGATAACGTTGCTGTTCAAAATTACCCCACTGTGACCGTCACAACCAACTACTCCACTATGTTGCTACCTTCTTCTTCCAACAATCTCAACAATTCCTCAAACTCCAATGCTCCAAACTACACTCATTTTCAACAACCACTTGTTGAGGAACAAAACCATGTTCCTAATATTTGTGGTTTTTCTGGTTTACCACTTTTTCCATCTCAAAACCAACCCAACAGAACCAGCAACAGAAACAGTAACTGTAACATAAGTTCTGGTACTAATATTGTTGATGTTGTTAACTCTTCACCTTCTATGATGGATGAAACCTCAGCTGCAACAAACTGGATTGATGGTATTTTGAAGGATCTTATTCATACTTCAAACAGTGTTTCCATTCCTCAACTTATCAACAATGTTAGAGAGATTATATATCCTTGTAATCCAAACCTTGCTCTTGTTCTTGAACATAGGTTACGTCTTCTTACAGAAACTGCTCCAAGTGTTGTTCCTGAGAGAAAAAGAAACAACACTGAACAACAAAGTGTTGCTAATGTTAATGTTTTACCTGCTTCTAATGTTAATTCCTCAGTGAAACTCATGAACCGTGTTGATGACATTGTTCCTCATTTTTCTGATTCATCAAGTCTTTTGAATCAAAATCAAAACATGTTTCCTAATTGGGGTGTGCCACAAATCAATAACAACAATAACCCTTTGGTTACTTTACCTTCACAACCACAATCAACACAACAAGATCAACATCAACAACATCAACATCAAGAGCATCAAGAAGATCTTGCACCTGCAACAACACCACCACCACCAACTTCTGCTGAGTTGGCAGTAACAAGAAAGAAGAAAGAGGAGATTAAAGAACAAAAGAAGAAAGATGAAGAAGGTTTACACCTTCTAACATTACTTCTTCAATGTGCAGAAGCTGTATCAGCAGAGAATCTTGAACAAGCAAACAAGATGTTACTCGAAATTTCGCAGCTTTCGACGCCTTTCGGCACATCAGCACAAAGAGTAGCAGCTTATTTCTCAGAAGCAATTTCAGCAAGACTAGTGAGTTCATGTCTAGGAATCTATGCAACACTTCCACCACATACACTACACAATCAAAAAGTTGCTTCAGCTTTTCAAGTCTTCAATGGTATTAGTCCATTTGTGAAATTCTCTCATTTCACTGCTAACCAAGCTATACAAGAAGCTTTTGATAGAGAAGAAAGAGTTCATATCATAGATCTTGATATCATGCAAGGTTTACAATGGCCTGGTCTTTTTCATATCCTTGCTTCAAGACCAGGTGGACCTCCTTATGTGAGACTCACTGGATTAGGTACTTCCATGGAAACACTTGAAGCTACTGGTAAACGTTTGTCTGATTTTGCATCTAAACTTGGTCTTCCTTTTGAGTTCTTTCCTGTTGCTGAGAAAGTTGGAAACATTGATGTTGAGAAGCTTAATGTTAGTAAATCAGAAGCTGTTGCTGTTCATTGGTTGCAACATTCTCTATATGATGTTACTGGTTCAGATACAAACACTTTGTGGCTCTTGCAAAGGTTGGTAATAGTAATAAAAGTTTAGGTGCATATACTCTCCATGAAATGATGTTATAGTTTGTTATTGTTACTCTTTTCCAATTCTTGTTGGTTTTGTATATATAATCTACATGATTTAGTGGTTGCTGATATGGAAAAATAGCACTTTTTCATGAAAGATTTCCCAAAACTTTATGCTTTTTTTTTACCTTTATAGACTATAGTGCTCACTTTATGTCTACCTTTTTTCTCTTCTAAAGGTAAAATTGGAAGCTATGATTATGTCTTTGTATAAATTAATAGATAGAAACAAAAAGAACTATATGTTATTATGATATGTTTATAGTAAGTTTTGAAAGACATAGCTCTCATGTAAGTTGATTATTACAATTTACAGACATGACATTAATATGTCTATAGTTTCTAATTATCCATTGGATTTGCATGGGATTTTTATTTTGGTTATGTGTTTATCTCTGTCTCTCTTACTTTTTATCTTTTACGAATTACGCACATTTCAAAAATACACAAATAAGAGGACACCGTATTTGAAATCAAAATTTCGATGTCAAAAAATCATTTATCTGTGTTATATATGTAGGTTGGCTCCTAAAGTGGTAACAGTAGTGGAACAAGACTTGAGCAATGCAGGTTCATTCTTGGGAAGGTTTGTGGAAGCAATACATTACTACTCAGCATTATTTGATTCTCTTGGTTCAAGCTATGGAGAAGAGAGTGAAGAGAGACATGTGGTGGAGCAGCAACTATTATCAAGAGAGATTCGTAACGTTCTTGCAGTTGGTGGACCATCAAGGAGTGGTGAGATAAAGTTTCACAATTGGAGAGAAAAGCTTCAACAGTGTGGTTTTAGAGGTATCTCTTTGGCTGGTAATGCTGCTACACAAGCTAGTCTTCTTCTTGGCATGTTCCCTTCTGAAGGGTATACTTTGGTTGAGGATAATGGTATCCTTAAACTTGGTTGGAAAGATCTTTGTTTGCTTACTGCTTCTGCTTGGAGACCTCCCTTTCATACCAACAATGTCATACCACATCATAACTGA
Protein sequence

>MS.gene016253.t1

MAACALFNGATTDETNNNSASNSSNISSEDLHTLHNNNTNMPQQQQQQQPHSERKVLRKRMASEMELQLQVHNNNNRFSRRNNNNSSNNISSLNCTLPSSTEKGVTTTTTLAAGTNNNNNNFHYNTNNINNSNNISHHSNVALSRDNVAVQNYPTVTVTTNYSTMLLPSSSNNLNNSSNSNAPNYTHFQQPLVEEQNHVPNICGFSGLPLFPSQNQPNRTSNRNSNCNISSGTNIVDVVNSSPSMMDETSAATNWIDGILKDLIHTSNSVSIPQLINNVREIIYPCNPNLALVLEHRLRLLTETAPSVVPERKRNNTEQQSVANVNVLPASNVNSSVKLMNRVDDIVPHFSDSSSLLNQNQNMFPNWEAVSAENLEQANKMLLEISQLSTPFGTSAQRVAAYFSEAISARLVSSCLGIYATLPPHTLHNQKVASAFQVFNGISPFVKFSHFTANQAIQEAFDREERVHIIDLDIMQGLQWPGLFHILASRPGGPPYVRLTGLGTSMETLEATGKRLSDFASKLGLPFEFFPVAEKVGNIDVEKLNVSKSEAVAVHWLQHSLYDVTGSDTNTLWLLQRLAPKVVTVVEQDLSNAGSFLGRFVEAIHYYSALFDSLGSSYGEESEERHVVEQQLLSREIRNVLAVGGPSRSGEIKFHNWREKLQQCGFRGISLAGNAATQASLLLGMFPSEGYTLVEDNGILKLGWKDLCLLTASAWRPPFHTNNVIPHHN