AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016358

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016358.t1 MTR_8g044220 91.563 403 23 2 1 403 48 439 0.0 758
MS.gene016358.t1 MTR_6g012450 36.869 396 185 13 56 402 115 494 1.05e-66 220
MS.gene016358.t1 MTR_4g082060 37.302 378 171 14 65 401 111 463 2.28e-62 208
MS.gene016358.t1 MTR_7g010950 35.171 381 191 10 62 402 121 485 2.45e-62 208
MS.gene016358.t1 MTR_3g435480 35.392 421 202 15 22 401 72 463 2.59e-60 202
MS.gene016358.t1 MTR_7g086780 36.939 379 163 9 61 403 84 422 1.04e-58 197
MS.gene016358.t1 MTR_5g041380 33.333 342 129 14 70 403 121 371 1.89e-43 155
MS.gene016358.t1 MTR_5g041400 32.609 368 159 12 39 403 82 363 2.99e-43 155
MS.gene016358.t1 MTR_6g027450 34.386 285 136 9 24 275 32 298 2.12e-36 135
MS.gene016358.t1 MTR_6g027460 39.623 159 64 2 64 190 84 242 3.94e-34 130
MS.gene016358.t1 MTR_2g016030 75.000 64 16 0 66 129 37 100 2.32e-32 120
MS.gene016358.t1 MTR_5g041420 64.198 81 29 0 51 131 113 193 2.25e-31 122
MS.gene016358.t1 MTR_5g041530 62.338 77 29 0 53 129 117 193 1.16e-29 118
MS.gene016358.t1 MTR_5g041530 32.973 185 73 9 230 403 237 381 2.56e-14 74.3
MS.gene016358.t1 MTR_4g022370 67.797 59 19 0 72 130 78 136 5.35e-25 105
MS.gene016358.t1 MTR_3g091820 59.701 67 27 0 65 131 32 98 1.05e-24 103
MS.gene016358.t1 MTR_2g059540 74.000 50 13 0 75 124 30 79 1.17e-24 100
MS.gene016358.t1 MTR_4g109980 71.698 53 15 0 74 126 38 90 3.50e-24 102
MS.gene016358.t1 MTR_8g079060 60.606 66 25 1 76 141 29 93 7.48e-24 99.4
MS.gene016358.t1 MTR_8g027295 61.290 62 24 0 71 132 19 80 8.12e-24 100
MS.gene016358.t1 MTR_2g096740 65.000 60 21 0 76 135 62 121 1.08e-23 100
MS.gene016358.t1 MTR_4g063780 66.102 59 20 0 72 130 71 129 1.23e-23 100
MS.gene016358.t1 MTR_2g096740 67.857 56 18 0 76 131 77 132 1.33e-23 100
MS.gene016358.t1 MTR_8g479350 72.222 54 15 0 76 129 68 121 2.31e-23 100
MS.gene016358.t1 MTR_2g093220 63.333 60 22 0 71 130 35 94 2.45e-23 99.4
MS.gene016358.t1 MTR_8g015840 69.231 52 16 0 76 127 20 71 6.25e-23 96.3
MS.gene016358.t1 MTR_8g068210 46.914 81 43 0 50 130 6 86 9.50e-23 98.6
MS.gene016358.t1 MTR_7g059400 69.811 53 16 0 78 130 78 130 1.62e-22 98.2
MS.gene016358.t1 MTR_1g077600 56.452 62 27 0 70 131 37 98 2.36e-22 96.3
MS.gene016358.t1 MTR_1g077600 59.322 59 24 0 70 128 37 95 2.78e-22 96.3
MS.gene016358.t1 MTR_7g024670 69.811 53 16 0 78 130 79 131 4.52e-22 97.1
MS.gene016358.t1 MTR_4g088580 67.925 53 17 0 78 130 57 109 8.53e-22 96.7
MS.gene016358.t1 MTR_5g031440 65.455 55 19 0 72 126 39 93 8.58e-22 95.9
MS.gene016358.t1 MTR_4g461080 60.000 65 25 1 65 128 17 81 3.99e-21 92.8
MS.gene016358.t1 MTR_3g077750 53.521 71 32 1 57 126 41 111 8.09e-21 92.8
MS.gene016358.t1 MTR_4g089095 65.455 55 18 1 76 130 50 103 9.96e-21 92.0
MS.gene016358.t1 MTR_4g089095 65.455 55 18 1 76 130 16 69 1.28e-20 90.9
MS.gene016358.t1 MTR_3g090430 64.151 53 19 0 78 130 53 105 1.37e-20 92.4
MS.gene016358.t1 MTR_2g014170 68.000 50 16 0 75 124 44 93 1.55e-20 91.7
MS.gene016358.t1 MTR_2g014060 68.000 50 16 0 78 127 27 76 1.57e-20 92.0
MS.gene016358.t1 MTR_1g056810 64.151 53 19 0 78 130 14 66 2.26e-20 90.9
MS.gene016358.t1 MTR_2g013370 66.038 53 17 1 78 130 48 99 3.30e-20 90.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016358.t1 AT5G39660 37.371 388 181 12 41 402 105 456 5.41e-61 204
MS.gene016358.t1 AT5G39660 37.371 388 181 12 41 402 105 456 5.41e-61 204
MS.gene016358.t1 AT5G39660 37.371 388 181 12 41 402 105 456 5.41e-61 204
MS.gene016358.t1 AT3G47500 37.008 381 176 16 63 403 92 448 1.71e-58 197
MS.gene016358.t1 AT5G62430 33.696 368 150 13 41 403 20 298 1.35e-44 157
MS.gene016358.t1 AT1G69570 30.788 406 177 12 27 403 67 397 5.65e-43 155
MS.gene016358.t1 AT1G26790 30.194 361 168 15 53 403 86 372 3.49e-36 136
MS.gene016358.t1 AT1G29160 69.863 73 22 0 57 129 43 115 3.00e-34 125
MS.gene016358.t1 AT2G34140 76.667 60 14 0 70 129 52 111 2.05e-32 120
MS.gene016358.t1 AT5G60850 66.667 69 21 1 76 144 51 117 1.76e-26 108
MS.gene016358.t1 AT3G55370 68.333 60 19 0 72 131 72 131 6.00e-25 104
MS.gene016358.t1 AT3G55370 68.333 60 19 0 72 131 72 131 6.81e-25 104
MS.gene016358.t1 AT3G55370 68.333 60 19 0 72 131 117 176 9.93e-25 104
MS.gene016358.t1 AT1G51700 68.421 57 17 1 76 131 33 89 1.77e-24 100
MS.gene016358.t1 AT2G37590 65.000 60 21 0 72 131 85 144 1.93e-24 103
MS.gene016358.t1 AT2G28810 69.643 56 17 0 72 127 73 128 2.83e-24 102
MS.gene016358.t1 AT2G28810 67.241 58 19 0 70 127 88 145 4.93e-24 102
MS.gene016358.t1 AT3G45610 50.633 79 34 1 58 131 17 95 5.31e-24 100
MS.gene016358.t1 AT5G60200 61.667 60 23 0 72 131 51 110 1.10e-23 99.8
MS.gene016358.t1 AT5G65590 51.685 89 40 1 43 131 11 96 1.42e-23 100
MS.gene016358.t1 AT3G61850 51.765 85 39 1 71 153 57 141 1.62e-23 99.8
MS.gene016358.t1 AT3G61850 51.765 85 39 1 71 153 57 141 1.62e-23 99.8
MS.gene016358.t1 AT3G61850 51.765 85 39 1 71 153 69 153 1.71e-23 99.8
MS.gene016358.t1 AT1G07640 69.091 55 17 0 76 130 21 75 2.01e-23 99.4
MS.gene016358.t1 AT3G50410 50.000 80 30 1 61 130 5 84 2.14e-23 98.6
MS.gene016358.t1 AT3G21270 69.811 53 16 0 76 128 29 81 2.31e-23 97.4
MS.gene016358.t1 AT4G38000 68.966 58 17 1 75 132 40 96 2.31e-23 98.2
MS.gene016358.t1 AT3G61850 51.765 85 39 1 71 153 69 153 2.59e-23 100
MS.gene016358.t1 AT3G61850 51.765 85 39 1 71 153 57 141 2.74e-23 99.8
MS.gene016358.t1 AT1G07640 69.091 55 17 0 76 130 77 131 3.67e-23 99.8
MS.gene016358.t1 AT1G07640 69.091 55 17 0 76 130 85 139 4.49e-23 99.4
MS.gene016358.t1 AT5G02460 66.102 59 20 0 72 130 91 149 6.08e-23 100
MS.gene016358.t1 AT4G24060 56.061 66 29 0 65 130 42 107 7.92e-23 99.0
MS.gene016358.t1 AT1G64620 69.231 52 16 0 73 124 46 97 1.82e-22 98.2
MS.gene016358.t1 AT4G00940 58.462 65 27 0 71 135 63 127 4.12e-22 95.9
MS.gene016358.t1 AT4G00940 58.462 65 27 0 71 135 63 127 4.12e-22 95.9
MS.gene016358.t1 AT4G00940 58.462 65 27 0 71 135 63 127 4.12e-22 95.9
MS.gene016358.t1 AT2G28510 68.519 54 17 0 78 131 50 103 5.57e-22 95.5
MS.gene016358.t1 AT5G66940 53.623 69 24 1 62 130 26 86 1.06e-21 93.2
MS.gene016358.t1 AT1G47655 69.388 49 15 0 76 124 29 77 1.33e-21 92.4
MS.gene016358.t1 AT1G28310 65.455 55 19 0 76 130 27 81 1.49e-21 94.7
MS.gene016358.t1 AT1G28310 65.455 55 19 0 76 130 51 105 2.25e-21 94.7
MS.gene016358.t1 AT4G21050 44.944 89 47 1 63 149 11 99 3.82e-21 91.3
MS.gene016358.t1 AT2G46590 64.151 53 19 0 71 123 63 115 1.28e-20 92.8
MS.gene016358.t1 AT2G46590 64.151 53 19 0 71 123 75 127 1.35e-20 92.8
MS.gene016358.t1 AT3G52440 62.264 53 20 0 78 130 27 79 2.77e-20 89.7
MS.gene016358.t1 AT3G52440 62.264 53 20 0 78 130 46 98 3.17e-20 90.1
MS.gene016358.t1 AT1G21340 61.111 54 21 0 78 131 40 93 4.70e-20 89.4
MS.gene016358.t1 AT5G62940 65.306 49 17 0 78 126 75 123 1.81e-19 89.7
MS.gene016358.t1 AT4G21040 67.347 49 16 0 78 126 27 75 4.35e-18 83.2
MS.gene016358.t1 AT4G21080 65.306 49 17 0 78 126 27 75 2.33e-17 81.6
MS.gene016358.t1 AT4G21030 61.364 44 17 0 78 121 23 66 2.33e-12 65.9

Find 94 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCGCTCCGGATCCTTTC+AGG 0.087266 8.2:-57171031 MS.gene016358:CDS
ATACAGTGAAGCAGAATATT+TGG 0.207890 8.2:-57171248 MS.gene016358:CDS
ATTGGGGTTGTATGCCAAAT+TGG 0.209941 8.2:-57171404 MS.gene016358:CDS
AAAACACTTCCTTTCATAAT+AGG 0.219092 8.2:+57171135 None:intergenic
TCCATTTGGCCAACCCAATT+TGG 0.234208 8.2:+57171390 None:intergenic
GAACTCTTAGCAGCCTCTTC+TGG 0.248749 8.2:+57171198 None:intergenic
GGACTTGAAGTTATGTTATC+AGG 0.270788 8.2:+57171468 None:intergenic
ACCGATGACACCTTCATATT+GGG 0.274088 8.2:-57171421 MS.gene016358:CDS
TTGCTTATTTCTACGCTTAC+CGG 0.293241 8.2:+57171842 None:intergenic
GGTAATAGGTCACCAACTTT+AGG 0.303102 8.2:-57171306 MS.gene016358:CDS
TTGATTGTCAGTATCTCTTT+TGG 0.303295 8.2:+57172037 None:intergenic
GGACAGCTGGTGGAGTAATT+AGG 0.315052 8.2:-57171881 MS.gene016358:CDS
TACCGATGACACCTTCATAT+TGG 0.324325 8.2:-57171422 MS.gene016358:CDS
GTGGTACTTGTTCCGGTAAT+AGG 0.368265 8.2:-57171320 MS.gene016358:CDS
TTGTGGATTAGCTTGTTTGC+TGG 0.369067 8.2:+57172151 None:intergenic
GCCAAATTGGGTTGGCCAAA+TGG 0.379899 8.2:-57171391 MS.gene016358:CDS
CATTCCATCCCCTTCATCTT+CGG 0.385022 8.2:-57171349 MS.gene016358:CDS
GCTAGCAGTGGTACTTGTTC+CGG 0.387977 8.2:-57171327 MS.gene016358:CDS
GAAAGTGGAATAGAGCATAT+TGG 0.393412 8.2:-57171597 MS.gene016358:CDS
CTAACGATAATTCTTCCATT+TGG 0.407757 8.2:+57171376 None:intergenic
ATTAGTTCTATGAACTGCTT+TGG 0.409555 8.2:+57172073 None:intergenic
GTACCGGTATTGTACATGTT+TGG 0.410276 8.2:+57172110 None:intergenic
GTAATTAGGAATGTTCCTAT+TGG 0.410965 8.2:-57171867 MS.gene016358:CDS
TCTACTGCCATCATGGTTGC+AGG 0.411890 8.2:+57171447 None:intergenic
GGTACTGCATCAAGGGTAAC+AGG 0.411943 8.2:+57171795 None:intergenic
CGAGAACTTGCATTTGACTT+AGG 0.412589 8.2:+57171099 None:intergenic
AGCTTCTGAATCCAAGGAAA+TGG 0.414730 8.2:-57171628 MS.gene016358:CDS
CTACGCTTACCGGCTCCAAT+AGG 0.417710 8.2:+57171852 None:intergenic
TTCAGAATACTCCATTTCCT+TGG 0.422020 8.2:+57171617 None:intergenic
TAAAGTTGGTGACCTATTAC+CGG 0.424336 8.2:+57171308 None:intergenic
GGACACGGCAGAACCTTGTC+TGG 0.427129 8.2:+57171996 None:intergenic
TACAGTGAAGCAGAATATTT+GGG 0.435846 8.2:-57171247 MS.gene016358:CDS
GTTTCTACCGATTCACTAAG+AGG 0.436362 8.2:+57171741 None:intergenic
CTATGAACTGCTTTGGTCAC+CGG 0.437272 8.2:+57172080 None:intergenic
GTGTCCTCGCTGCAATAGTT+TGG 0.447623 8.2:-57171979 MS.gene016358:CDS
CACGCTTTCCTGAAAGGATC+CGG 0.450006 8.2:+57171023 None:intergenic
GACCTATCCATGTTGGAATC+CGG 0.461640 8.2:-57171502 MS.gene016358:CDS
CAATACCGGTACTTTGAAAC+CGG 0.464327 8.2:-57172099 MS.gene016358:CDS
TTGGGGTTGTATGCCAAATT+GGG 0.467016 8.2:-57171403 MS.gene016358:CDS
TGCCAGAGGTATTGGACAGC+TGG 0.480992 8.2:-57171894 MS.gene016358:CDS
CCGGAGCGAGAGAAAGCAGC+AGG 0.481752 8.2:+57171042 None:intergenic
ATTATTACTCTGCTCCTCCA+TGG 0.483782 8.2:-57171524 MS.gene016358:CDS
CGGTACTTCTACTGCCATCA+TGG 0.503743 8.2:+57171440 None:intergenic
TTAGGAAAACATTGTAGAGA+TGG 0.504456 8.2:-57171288 MS.gene016358:CDS
GGAAGTTCCTCTTAGTGAAT+CGG 0.506714 8.2:-57171748 MS.gene016358:CDS
GGTTGTATGCCAAATTGGGT+TGG 0.508787 8.2:-57171399 MS.gene016358:CDS
AGAAACTGTGTTAAATCTCA+AGG 0.509219 8.2:-57171724 MS.gene016358:CDS
AGGCTGCTAAGAGTTCTATA+TGG 0.510382 8.2:-57171191 MS.gene016358:CDS
ACTAAGAGGAACTTCCTCCT+TGG 0.511294 8.2:+57171755 None:intergenic
CCTGCTGCTTTCTCTCGCTC+CGG 0.514923 8.2:-57171042 MS.gene016358:CDS
CACCAGCTGTCCAATACCTC+TGG 0.521554 8.2:+57171892 None:intergenic
GAAATGGAGTATTCTGAAAG+TGG 0.522372 8.2:-57171612 MS.gene016358:CDS
AGAAGCTCTCACATAAGAGC+TGG 0.523464 8.2:+57171644 None:intergenic
GGTCACCGGTTTCAAAGTAC+CGG 0.526533 8.2:+57172094 None:intergenic
CACTGCTAGCCGAAGATGAA+GGG 0.530871 8.2:+57171340 None:intergenic
AGGAATGTTCCTATTGGAGC+CGG 0.533831 8.2:-57171861 MS.gene016358:CDS
CTCCATGGACCTATCCATGT+TGG 0.535549 8.2:-57171509 MS.gene016358:CDS
GGCATACAACCCCAATATGA+AGG 0.535682 8.2:+57171411 None:intergenic
ATATTACACGCTTTCCTGAA+AGG 0.538533 8.2:+57171017 None:intergenic
TTGGAATCTATGTGTATGAC+AGG 0.546648 8.2:+57171774 None:intergenic
CCACTGCTAGCCGAAGATGA+AGG 0.549382 8.2:+57171339 None:intergenic
ATTCCAAACATGTACAATAC+CGG 0.552268 8.2:-57172113 MS.gene016358:CDS
TGTGAGAGCTTCTGAATCCA+AGG 0.552985 8.2:-57171634 MS.gene016358:CDS
TAATAATGAAGTGAATGTAA+AGG 0.559539 8.2:+57171540 None:intergenic
CAGAATTAATGATCCAGAAG+AGG 0.559620 8.2:-57171211 MS.gene016358:CDS
TTATCAGGCTTAAATGCCAC+CGG 0.562271 8.2:+57171483 None:intergenic
TGTCATACACATAGATTCCA+AGG 0.569094 8.2:-57171772 MS.gene016358:CDS
TATGACAGGTACTGCATCAA+GGG 0.571285 8.2:+57171788 None:intergenic
TTCAAGTCCTGCAACCATGA+TGG 0.572277 8.2:-57171454 MS.gene016358:CDS
CCCCAATATGAAGGTGTCAT+CGG 0.575122 8.2:+57171420 None:intergenic
CAGAACAAACCTATTATGAA+AGG 0.575243 8.2:-57171144 MS.gene016358:CDS
AAACTATTGCAGCGAGGACA+CGG 0.577128 8.2:+57171981 None:intergenic
ATAGTATTAGCATCTTCTTG+TGG 0.578337 8.2:+57172134 None:intergenic
ATGGAAGAATTATCGTTAGT+CGG 0.583127 8.2:-57171372 MS.gene016358:CDS
TTCCAACATGGATAGGTCCA+TGG 0.586329 8.2:+57171507 None:intergenic
ACTTTGACACCACAATGCAA+TGG 0.588024 8.2:-57171570 MS.gene016358:CDS
GTATGACAGGTACTGCATCA+AGG 0.597457 8.2:+57171787 None:intergenic
CCGATGACACCTTCATATTG+GGG 0.607080 8.2:-57171420 MS.gene016358:CDS
CTAGCCGAAGATGAAGGGGA+TGG 0.608686 8.2:+57171345 None:intergenic
GAAACTGTGTTAAATCTCAA+GGG 0.609481 8.2:-57171723 MS.gene016358:CDS
CCTTCATCTTCGGCTAGCAG+TGG 0.611094 8.2:-57171339 MS.gene016358:CDS
CAGAGGTATTGGACAGCTGG+TGG 0.613246 8.2:-57171891 MS.gene016358:CDS
ATTACAATGTTAACCAACCA+AGG 0.617440 8.2:-57171932 MS.gene016358:CDS
GGACTCTTCTACTGTCAAAG+AGG 0.618008 8.2:-57171682 MS.gene016358:CDS
CACCGGATTCCAACATGGAT+AGG 0.620558 8.2:+57171500 None:intergenic
GGAATTAAGCCATTGCATTG+TGG 0.632188 8.2:+57171561 None:intergenic
ACTGCTAGCCGAAGATGAAG+GGG 0.636553 8.2:+57171341 None:intergenic
GGAACTTGACAATTCTGCAA+AGG 0.639283 8.2:+57171816 None:intergenic
AATGCCACCGGATTCCAACA+TGG 0.642061 8.2:+57171495 None:intergenic
CTATCCATGTTGGAATCCGG+TGG 0.644273 8.2:-57171499 MS.gene016358:CDS
CGGAGCGAGAGAAAGCAGCA+GGG 0.655440 8.2:+57171043 None:intergenic
GTCTCCAAACTATTGCAGCG+AGG 0.702604 8.2:+57171975 None:intergenic
CAACATGGATAGGTCCATGG+AGG 0.727826 8.2:+57171510 None:intergenic
CATACACATAGATTCCAAGG+AGG 0.765853 8.2:-57171769 MS.gene016358:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAATGAAATATGTT+AGG - chr8.2:57171694-57171713 MS.gene016358:CDS 10.0%
!! AATATATAAGAAAGATAAAA+AGG + chr8.2:57171062-57171081 None:intergenic 10.0%
!!! TATCTTTCTTATATATTTTT+TGG - chr8.2:57171065-57171084 MS.gene016358:CDS 10.0%
!! AAAATGTTTATATTATAGCT+AGG - chr8.2:57171277-57171296 MS.gene016358:CDS 15.0%
!!! TTTGAAAAAGTCATTATTTT+CGG - chr8.2:57172012-57172031 MS.gene016358:CDS 15.0%
!! ATTCGTATATATATAGTTCT+AGG - chr8.2:57171196-57171215 MS.gene016358:CDS 20.0%
!! GAAAGGAAAAAAAAAAATCT+GGG + chr8.2:57171144-57171163 None:intergenic 20.0%
!! TAATAATGAAGTGAATGTAA+AGG + chr8.2:57172824-57172843 None:intergenic 20.0%
!! TGAAAGGAAAAAAAAAAATC+TGG + chr8.2:57171145-57171164 None:intergenic 20.0%
!! TGACATGAAAAATGTTAAAT+TGG - chr8.2:57171447-57171466 MS.gene016358:CDS 20.0%
!! TTTGAACTTAACATAACAAA+AGG + chr8.2:57171737-57171756 None:intergenic 20.0%
!!! GAATTATTTTTGTTTGAATC+TGG - chr8.2:57171389-57171408 MS.gene016358:CDS 20.0%
!!! TAATGACTTTTTCAAATTAG+CGG + chr8.2:57172008-57172027 None:intergenic 20.0%
!!! TATCAATGAGTTATAACTTT+TGG - chr8.2:57172071-57172090 MS.gene016358:CDS 20.0%
!!! TCATTAATTCTGATAGTTTT+AGG + chr8.2:57173142-57173161 None:intergenic 20.0%
!!! TGTTTGATAATTTGTTTTGA+AGG - chr8.2:57171310-57171329 MS.gene016358:CDS 20.0%
!!! TTTGGTATTGTTTAATTTGT+GGG - chr8.2:57171836-57171855 MS.gene016358:CDS 20.0%
!!! TTTTGGTATTGTTTAATTTG+TGG - chr8.2:57171835-57171854 MS.gene016358:CDS 20.0%
! AAAACACTTCCTTTCATAAT+AGG + chr8.2:57173229-57173248 None:intergenic 25.0%
! AAATCAACGTTGTTAAATAG+TGG - chr8.2:57171886-57171905 MS.gene016358:CDS 25.0%
! AAATTGGACCAAATTAATTG+AGG - chr8.2:57171463-57171482 MS.gene016358:CDS 25.0%
! ACTATAGAAAATAAGTGGTA+GGG - chr8.2:57171358-57171377 MS.gene016358:CDS 25.0%
! TACTATAGAAAATAAGTGGT+AGG - chr8.2:57171357-57171376 MS.gene016358:CDS 25.0%
! TTAAAAAGACAACTTGCATT+AGG + chr8.2:57172098-57172117 None:intergenic 25.0%
!! ACATTTTTCATGTCAATTAC+TGG + chr8.2:57171442-57171461 None:intergenic 25.0%
!! GTGTTACTATAGAAAATAAG+TGG - chr8.2:57171353-57171372 MS.gene016358:CDS 25.0%
!! TTAATTTTGTGTTAATCCTC+TGG + chr8.2:57171681-57171700 None:intergenic 25.0%
!! TTGATAGTATGCATTTCTAT+CGG + chr8.2:57172057-57172076 None:intergenic 25.0%
!!! TTGAATCTGGTTTTTTAAGT+TGG - chr8.2:57171402-57171421 MS.gene016358:CDS 25.0%
!!! TTTTTTCCTTTCATACTGAA+TGG - chr8.2:57171152-57171171 MS.gene016358:CDS 25.0%
AAATTAATTGAGGTGTGTTC+TGG - chr8.2:57171473-57171492 MS.gene016358:CDS 30.0%
AAGGTGCAAAAAAGATGAAA+AGG + chr8.2:57171043-57171062 None:intergenic 30.0%
AGAAACTGTGTTAAATCTCA+AGG - chr8.2:57172637-57172656 MS.gene016358:intron 30.0%
AGAACACACCTCAATTAATT+TGG + chr8.2:57171474-57171493 None:intergenic 30.0%
ATAATTCGAGTCCAATTACT+CGG + chr8.2:57171529-57171548 None:intergenic 30.0%
ATACAGTGAAGCAGAATATT+TGG - chr8.2:57173113-57173132 MS.gene016358:intron 30.0%
ATAGTATTAGCATCTTCTTG+TGG + chr8.2:57172230-57172249 None:intergenic 30.0%
ATGGAAGAATTATCGTTAGT+CGG - chr8.2:57172989-57173008 MS.gene016358:intron 30.0%
ATTACAATGTTAACCAACCA+AGG - chr8.2:57172429-57172448 MS.gene016358:intron 30.0%
ATTCCAAACATGTACAATAC+CGG - chr8.2:57172248-57172267 MS.gene016358:intron 30.0%
CAGAACAAACCTATTATGAA+AGG - chr8.2:57173217-57173236 MS.gene016358:intron 30.0%
CTAACGATAATTCTTCCATT+TGG + chr8.2:57172988-57173007 None:intergenic 30.0%
CTATATCCATTCAGTATGAA+AGG + chr8.2:57171161-57171180 None:intergenic 30.0%
GAAACTGTGTTAAATCTCAA+GGG - chr8.2:57172638-57172657 MS.gene016358:intron 30.0%
GGGCAATAGAAAAATTGAAA+CGG - chr8.2:57172658-57172677 MS.gene016358:intron 30.0%
GTAATTAGGAATGTTCCTAT+TGG - chr8.2:57172494-57172513 MS.gene016358:intron 30.0%
TAAACAATACCAAAAACTCG+TGG + chr8.2:57171830-57171849 None:intergenic 30.0%
TACAGTGAAGCAGAATATTT+GGG - chr8.2:57173114-57173133 MS.gene016358:intron 30.0%
TTAGGAAAACATTGTAGAGA+TGG - chr8.2:57173073-57173092 MS.gene016358:intron 30.0%
TTCATACTGAATGGATATAG+AGG - chr8.2:57171161-57171180 MS.gene016358:CDS 30.0%
TTGTACTAGATAATGTATCG+TGG + chr8.2:57171949-57171968 None:intergenic 30.0%
! CTACAATGTTTTCCTAAAGT+TGG + chr8.2:57173070-57173089 None:intergenic 30.0%
! TTGATTGTCAGTATCTCTTT+TGG + chr8.2:57172327-57172346 None:intergenic 30.0%
!! ATTAGTTCTATGAACTGCTT+TGG + chr8.2:57172291-57172310 None:intergenic 30.0%
ACTAAGTACGAATTGTTCGT+GGG - chr8.2:57171560-57171579 MS.gene016358:CDS 35.0%
ATCGTGGAACAACACTAATT+TGG + chr8.2:57171933-57171952 None:intergenic 35.0%
ATTTCTGCAAAAATTGCCAG+AGG - chr8.2:57172453-57172472 MS.gene016358:intron 35.0%
CAGAATTAATGATCCAGAAG+AGG - chr8.2:57173150-57173169 MS.gene016358:intron 35.0%
CTGTATCATTGGAATATGTC+TGG - chr8.2:57171499-57171518 MS.gene016358:CDS 35.0%
GAAAGTGGAATAGAGCATAT+TGG - chr8.2:57172764-57172783 MS.gene016358:intron 35.0%
GGACTTGAAGTTATGTTATC+AGG + chr8.2:57172896-57172915 None:intergenic 35.0%
TGTCATACACATAGATTCCA+AGG - chr8.2:57172589-57172608 MS.gene016358:intron 35.0%
TTCAGAATACTCCATTTCCT+TGG + chr8.2:57172747-57172766 None:intergenic 35.0%
TTGGAATCTATGTGTATGAC+AGG + chr8.2:57172590-57172609 None:intergenic 35.0%
TTTGGTACTCAATTGCAGAA+TGG - chr8.2:57172137-57172156 MS.gene016358:CDS 35.0%
! CAATTTTTGCAGAAATGCCT+TGG + chr8.2:57172449-57172468 None:intergenic 35.0%
! GAAATGGAGTATTCTGAAAG+TGG - chr8.2:57172749-57172768 MS.gene016358:intron 35.0%
! GTCAGACTTTACTTACGTTT+TGG - chr8.2:57171618-57171637 MS.gene016358:CDS 35.0%
! TGTTCTGGTTACTGTATCAT+TGG - chr8.2:57171488-57171507 MS.gene016358:CDS 35.0%
!! TAAAGTTGGTGACCTATTAC+CGG + chr8.2:57173056-57173075 None:intergenic 35.0%
!! TTGCTTATTTCTACGCTTAC+CGG + chr8.2:57172522-57172541 None:intergenic 35.0%
ACCGATGACACCTTCATATT+GGG - chr8.2:57172940-57172959 MS.gene016358:intron 40.0%
ACTTTGACACCACAATGCAA+TGG - chr8.2:57172791-57172810 MS.gene016358:intron 40.0%
AGCTTCTGAATCCAAGGAAA+TGG - chr8.2:57172733-57172752 MS.gene016358:intron 40.0%
AGGCTGCTAAGAGTTCTATA+TGG - chr8.2:57173170-57173189 MS.gene016358:intron 40.0%
ATTATTACTCTGCTCCTCCA+TGG - chr8.2:57172837-57172856 MS.gene016358:intron 40.0%
ATTGGGGTTGTATGCCAAAT+TGG - chr8.2:57172957-57172976 MS.gene016358:intron 40.0%
CACGAACAATTCGTACTTAG+TGG + chr8.2:57171561-57171580 None:intergenic 40.0%
CACTAAGTACGAATTGTTCG+TGG - chr8.2:57171559-57171578 MS.gene016358:CDS 40.0%
CATACACATAGATTCCAAGG+AGG - chr8.2:57172592-57172611 MS.gene016358:intron 40.0%
GGAACTTGACAATTCTGCAA+AGG + chr8.2:57172548-57172567 None:intergenic 40.0%
GGAAGTTCCTCTTAGTGAAT+CGG - chr8.2:57172613-57172632 MS.gene016358:intron 40.0%
GGAATTAAGCCATTGCATTG+TGG + chr8.2:57172803-57172822 None:intergenic 40.0%
GGTAATAGGTCACCAACTTT+AGG - chr8.2:57173055-57173074 MS.gene016358:intron 40.0%
TACCGATGACACCTTCATAT+TGG - chr8.2:57172939-57172958 MS.gene016358:intron 40.0%
TTAGCGGCATAACAGTTCTA+TGG + chr8.2:57171992-57172011 None:intergenic 40.0%
TTATCAGGCTTAAATGCCAC+CGG + chr8.2:57172881-57172900 None:intergenic 40.0%
TTCCTGAGTGGGATAATTCT+TGG - chr8.2:57171596-57171615 MS.gene016358:CDS 40.0%
TTGGGGTTGTATGCCAAATT+GGG - chr8.2:57172958-57172977 MS.gene016358:intron 40.0%
! CGAGAACTTGCATTTGACTT+AGG + chr8.2:57173265-57173284 None:intergenic 40.0%
! CGAGTAAAACCACGAGTTTT+TGG - chr8.2:57171818-57171837 MS.gene016358:CDS 40.0%
! GCAAAAATTGCCAGAGGTAT+TGG - chr8.2:57172459-57172478 MS.gene016358:intron 40.0%
! GTACCGGTATTGTACATGTT+TGG + chr8.2:57172254-57172273 None:intergenic 40.0%
! GTTAATCCTCTGGTTCCTAA+GGG + chr8.2:57171671-57171690 None:intergenic 40.0%
! TATGACAGGTACTGCATCAA+GGG + chr8.2:57172576-57172595 None:intergenic 40.0%
! TCTATCGGTGCTTTCAATCA+CGG + chr8.2:57172042-57172061 None:intergenic 40.0%
! TGTTAATCCTCTGGTTCCTA+AGG + chr8.2:57171672-57171691 None:intergenic 40.0%
! TTTTGCAGAAATGCCTTGGT+TGG + chr8.2:57172445-57172464 None:intergenic 40.0%
!! AGTTTTCAAGAAGCCAGACA+AGG - chr8.2:57172352-57172371 MS.gene016358:intron 40.0%
!! CAATACCGGTACTTTGAAAC+CGG - chr8.2:57172262-57172281 MS.gene016358:intron 40.0%
!! GTTTCTACCGATTCACTAAG+AGG + chr8.2:57172623-57172642 None:intergenic 40.0%
!! TTGTGGATTAGCTTGTTTGC+TGG + chr8.2:57172213-57172232 None:intergenic 40.0%
AAACTATTGCAGCGAGGACA+CGG + chr8.2:57172383-57172402 None:intergenic 45.0%
ACTAAGAGGAACTTCCTCCT+TGG + chr8.2:57172609-57172628 None:intergenic 45.0%
AGAAGCTCTCACATAAGAGC+TGG + chr8.2:57172720-57172739 None:intergenic 45.0%
AGGAATGTTCCTATTGGAGC+CGG - chr8.2:57172500-57172519 MS.gene016358:intron 45.0%
ATCTGAGTTCGATTCCTGAG+TGG - chr8.2:57171584-57171603 MS.gene016358:CDS 45.0%
CATTCCATCCCCTTCATCTT+CGG - chr8.2:57173012-57173031 MS.gene016358:intron 45.0%
CCGATGACACCTTCATATTG+GGG - chr8.2:57172941-57172960 MS.gene016358:intron 45.0%
CTATGAACTGCTTTGGTCAC+CGG + chr8.2:57172284-57172303 None:intergenic 45.0%
GACCAAGAATTATCCCACTC+AGG + chr8.2:57171601-57171620 None:intergenic 45.0%
GACCTATCCATGTTGGAATC+CGG - chr8.2:57172859-57172878 MS.gene016358:intron 45.0%
GGACTCTTCTACTGTCAAAG+AGG - chr8.2:57172679-57172698 MS.gene016358:intron 45.0%
GGCATACAACCCCAATATGA+AGG + chr8.2:57172953-57172972 None:intergenic 45.0%
GGTTGTATGCCAAATTGGGT+TGG - chr8.2:57172962-57172981 MS.gene016358:intron 45.0%
GTGGTACTTGTTCCGGTAAT+AGG - chr8.2:57173041-57173060 MS.gene016358:intron 45.0%
TCCATTTGGCCAACCCAATT+TGG + chr8.2:57172974-57172993 None:intergenic 45.0%
TCTGAGTTCGATTCCTGAGT+GGG - chr8.2:57171585-57171604 MS.gene016358:CDS 45.0%
TGTCTGGTTGACCGAGTAAT+TGG - chr8.2:57171515-57171534 MS.gene016358:CDS 45.0%
TGTGAGAGCTTCTGAATCCA+AGG - chr8.2:57172727-57172746 MS.gene016358:intron 45.0%
TTCAAGTCCTGCAACCATGA+TGG - chr8.2:57172907-57172926 MS.gene016358:intron 45.0%
TTCCAACATGGATAGGTCCA+TGG + chr8.2:57172857-57172876 None:intergenic 45.0%
! GTATGACAGGTACTGCATCA+AGG + chr8.2:57172577-57172596 None:intergenic 45.0%
!! CCCCAATATGAAGGTGTCAT+CGG + chr8.2:57172944-57172963 None:intergenic 45.0%
AATGCCACCGGATTCCAACA+TGG + chr8.2:57172869-57172888 None:intergenic 50.0%
ACTGCTAGCCGAAGATGAAG+GGG + chr8.2:57173023-57173042 None:intergenic 50.0%
CAACATGGATAGGTCCATGG+AGG + chr8.2:57172854-57172873 None:intergenic 50.0%
CACCGGATTCCAACATGGAT+AGG + chr8.2:57172864-57172883 None:intergenic 50.0%
CACGCTTTCCTGAAAGGATC+CGG + chr8.2:57173341-57173360 None:intergenic 50.0%
CACTGCTAGCCGAAGATGAA+GGG + chr8.2:57173024-57173043 None:intergenic 50.0%
CGGTACTTCTACTGCCATCA+TGG + chr8.2:57172924-57172943 None:intergenic 50.0%
CGTTCCAGTAATCCGGAGTT+CGG + chr8.2:57171644-57171663 None:intergenic 50.0%
CTATCCATGTTGGAATCCGG+TGG - chr8.2:57172862-57172881 MS.gene016358:intron 50.0%
CTCCATGGACCTATCCATGT+TGG - chr8.2:57172852-57172871 MS.gene016358:intron 50.0%
GAACTCTTAGCAGCCTCTTC+TGG + chr8.2:57173166-57173185 None:intergenic 50.0%
GCTAGCAGTGGTACTTGTTC+CGG - chr8.2:57173034-57173053 MS.gene016358:intron 50.0%
GGACAGCTGGTGGAGTAATT+AGG - chr8.2:57172480-57172499 MS.gene016358:intron 50.0%
GGTACTGCATCAAGGGTAAC+AGG + chr8.2:57172569-57172588 None:intergenic 50.0%
GTCTCCAAACTATTGCAGCG+AGG + chr8.2:57172389-57172408 None:intergenic 50.0%
GTGTCCTCGCTGCAATAGTT+TGG - chr8.2:57172382-57172401 MS.gene016358:intron 50.0%
TCTACTGCCATCATGGTTGC+AGG + chr8.2:57172917-57172936 None:intergenic 50.0%
TTACTGGAACGCCTTCCCTT+AGG - chr8.2:57171653-57171672 MS.gene016358:CDS 50.0%
! ATCCTCTGGTTCCTAAGGGA+AGG + chr8.2:57171667-57171686 None:intergenic 50.0%
!! CTTACGTTTTGGCCGAACTC+CGG - chr8.2:57171629-57171648 MS.gene016358:CDS 50.0%
!! GCCAAATTGGGTTGGCCAAA+TGG - chr8.2:57172970-57172989 MS.gene016358:intron 50.0%
!! GGTCACCGGTTTCAAAGTAC+CGG + chr8.2:57172270-57172289 None:intergenic 50.0%
!! GTGTGTCGACTGACACAGTT+TGG - chr8.2:57172119-57172138 MS.gene016358:CDS 50.0%
CACCAGCTGTCCAATACCTC+TGG + chr8.2:57172472-57172491 None:intergenic 55.0%
CCACTGCTAGCCGAAGATGA+AGG + chr8.2:57173025-57173044 None:intergenic 55.0%
CCTTCATCTTCGGCTAGCAG+TGG - chr8.2:57173022-57173041 MS.gene016358:intron 55.0%
CTAGCCGAAGATGAAGGGGA+TGG + chr8.2:57173019-57173038 None:intergenic 55.0%
TTGGCCGAACTCCGGATTAC+TGG - chr8.2:57171637-57171656 MS.gene016358:CDS 55.0%
!! CAGAGGTATTGGACAGCTGG+TGG - chr8.2:57172470-57172489 MS.gene016358:intron 55.0%
!! CTACGCTTACCGGCTCCAAT+AGG + chr8.2:57172512-57172531 None:intergenic 55.0%
!! GGGAAGGCGTTCCAGTAATC+CGG + chr8.2:57171651-57171670 None:intergenic 55.0%
!! TGCCAGAGGTATTGGACAGC+TGG - chr8.2:57172467-57172486 MS.gene016358:intron 55.0%
CGCCTTCCCTTAGGAACCAG+AGG - chr8.2:57171662-57171681 MS.gene016358:CDS 60.0%
CGGAGCGAGAGAAAGCAGCA+GGG + chr8.2:57173321-57173340 None:intergenic 60.0%
CTCTCGCTCCGGATCCTTTC+AGG - chr8.2:57173330-57173349 MS.gene016358:intron 60.0%
GGACACGGCAGAACCTTGTC+TGG + chr8.2:57172368-57172387 None:intergenic 60.0%
!! CCTGCTGCTTTCTCTCGCTC+CGG - chr8.2:57173319-57173338 MS.gene016358:intron 60.0%
CCGGAGCGAGAGAAAGCAGC+AGG + chr8.2:57173322-57173341 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 57171020 57173363 57171020 ID=MS.gene016358
chr8.2 mRNA 57171020 57173363 57171020 ID=MS.gene016358.t1;Parent=MS.gene016358
chr8.2 exon 57173361 57173363 57173361 ID=MS.gene016358.t1.exon1;Parent=MS.gene016358.t1
chr8.2 CDS 57173361 57173363 57173361 ID=cds.MS.gene016358.t1;Parent=MS.gene016358.t1
chr8.2 exon 57171020 57172228 57171020 ID=MS.gene016358.t1.exon2;Parent=MS.gene016358.t1
chr8.2 CDS 57171020 57172228 57171020 ID=cds.MS.gene016358.t1;Parent=MS.gene016358.t1
Gene Sequence

>MS.gene016358

ATGGTCATGCTTTTCATTTTCCTTTTCATCTTTTTTGCACCTTTTTATCTTTCTTATATATTTTTTGGCTTGCACTTTACTTAATGCTAAAGTTTGCATTTTTATACTAAAAAATTAAAAACCCAGATTTTTTTTTTTCCTTTCATACTGAATGGATATAGAGGCTATTTATTATTATTCGTATATATATAGTTCTAGGTTGTATATACTTGTATAATAATGAATATTTTGATTTTGTTATTAATATCTATAACTCAAAAATGTTTATATTATAGCTAGGTATATGATGTTGTTTGATAATTTGTTTTGAAGGTAGTGAGACTTGACTTTTGAGTGTTACTATAGAAAATAAGTGGTAGGGTTCAATTTGAATTATTTTTGTTTGAATCTGGTTTTTTAAGTTGGACTGAACTGTTTCACCAGTAATTGACATGAAAAATGTTAAATTGGACCAAATTAATTGAGGTGTGTTCTGGTTACTGTATCATTGGAATATGTCTGGTTGACCGAGTAATTGGACTCGAATTATTAATGAACTCCACTAAGTACGAATTGTTCGTGGGAATCTGAGTTCGATTCCTGAGTGGGATAATTCTTGGTCAGACTTTACTTACGTTTTGGCCGAACTCCGGATTACTGGAACGCCTTCCCTTAGGAACCAGAGGATTAACACAAAATTAAAATGAAATATGTTAGGCGAAAGAATTTTTTTGACCTTTTGTTATGTTAAGTTCAAAATCGATTGCATTTTAACTTGATTCTTGTTGTCATATTCTTTAACTGTTTGATTACTTTGCTCGAGTAAAACCACGAGTTTTTGGTATTGTTTAATTTGTGGGTATTTCATGTATAGTTTTGATGTGTAGAAATCAACGTTGTTAAATAGTGGCTATAGCGTAGCAGATTTTGACCAAATTAGTGTTGTTCCACGATACATTATCTAGTACAAAATGTTGTCAAATAGCAGTGCCATAGAACTGTTATGCCGCTAATTTGAAAAAGTCATTATTTTCGGCGATCCGTGATTGAAAGCACCGATAGAAATGCATACTATCAATGAGTTATAACTTTTGGTCCTAATGCAAGTTGTCTTTTTAATGTGTGTCGACTGACACAGTTTGGTACTCAATTGCAGAATGGACTTGAAGAATCAGCTAATGCTGATGTTGATGTTCGTACGAATGAGCTTTCCAGCAAACAAGCTAATCCACAAGAAGATGCTAATACTATTCCAAACATGTACAATACCGGTACTTTGAAACCGGTGACCAAAGCAGTTCATAGAACTAATGAACACAAGACTGCCAAAAGAGATACTGACAATCAAGAGAAAGTTTTCAAGAAGCCAGACAAGGTTCTGCCGTGTCCTCGCTGCAATAGTTTGGAGACAAAGTTTTGCTATTTCAACAATTACAATGTTAACCAACCAAGGCATTTCTGCAAAAATTGCCAGAGGTATTGGACAGCTGGTGGAGTAATTAGGAATGTTCCTATTGGAGCCGGTAAGCGTAGAAATAAGCAATCGCCTTTGCAGAATTGTCAAGTTCCTGTTACCCTTGATGCAGTACCTGTCATACACATAGATTCCAAGGAGGAAGTTCCTCTTAGTGAATCGGTAGAAACTGTGTTAAATCTCAAGGGCAATAGAAAAATTGAAACGGACTCTTCTACTGTCAAAGAGGAAATTGAGAACTCTTCCAGCTCTTATGTGAGAGCTTCTGAATCCAAGGAAATGGAGTATTCTGAAAGTGGAATAGAGCATATTGGTTTGACTTTGACACCACAATGCAATGGCTTAATTCCTTTACATTCACTTCATTATTACTCTGCTCCTCCATGGACCTATCCATGTTGGAATCCGGTGGCATTTAAGCCTGATAACATAACTTCAAGTCCTGCAACCATGATGGCAGTAGAAGTACCGATGACACCTTCATATTGGGGTTGTATGCCAAATTGGGTTGGCCAAATGGAAGAATTATCGTTAGTCGGCATTCCATCCCCTTCATCTTCGGCTAGCAGTGGTACTTGTTCCGGTAATAGGTCACCAACTTTAGGAAAACATTGTAGAGATGGAAGTACACAAGAAGAAGATACAGTGAAGCAGAATATTTGGGTGCCTAAAACTATCAGAATTAATGATCCAGAAGAGGCTGCTAAGAGTTCTATATGGTCAACTTTGAGAACTAAATCTGAACAGAACAAACCTATTATGAAAGGAAGTGTTTTTAAATCATTTGAACCTAAGTCAAATGCAAGTTCTCGTGATTTAGACGATAATCAAATTCTACGAGCAAACCCTGCTGCTTTCTCTCGCTCCGGATCCTTTCAGGAAAGCGTGTAA
Protein sequence

>MS.gene016358.t1

MNGLEESANADVDVRTNELSSKQANPQEDANTIPNMYNTGTLKPVTKAVHRTNEHKTAKRDTDNQEKVFKKPDKVLPCPRCNSLETKFCYFNNYNVNQPRHFCKNCQRYWTAGGVIRNVPIGAGKRRNKQSPLQNCQVPVTLDAVPVIHIDSKEEVPLSESVETVLNLKGNRKIETDSSTVKEEIENSSSSYVRASESKEMEYSESGIEHIGLTLTPQCNGLIPLHSLHYYSAPPWTYPCWNPVAFKPDNITSSPATMMAVEVPMTPSYWGCMPNWVGQMEELSLVGIPSPSSSASSGTCSGNRSPTLGKHCRDGSTQEEDTVKQNIWVPKTIRINDPEEAAKSSIWSTLRTKSEQNKPIMKGSVFKSFEPKSNASSRDLDDNQILRANPAAFSRSGSFQESV