Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016579.t1 | PNX93400.1 | 63.9 | 285 | 89 | 1 | 66 | 336 | 3 | 287 | 2.10E-94 | 355.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016579.t1 | Q84MZ4 | 55.3 | 150 | 67 | 0 | 186 | 335 | 216 | 365 | 2.9e-44 | 180.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016579.t1 | A0A2K3MRG1 | 63.9 | 285 | 89 | 1 | 66 | 336 | 3 | 287 | 1.5e-94 | 355.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene016579.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016579.t1 | AT1G72050 | 49.701 | 334 | 109 | 4 | 58 | 335 | 35 | 365 | 1.23e-103 | 310 |
| MS.gene016579.t1 | AT1G72050 | 49.701 | 334 | 109 | 4 | 58 | 335 | 50 | 380 | 1.28e-103 | 311 |
| MS.gene016579.t1 | AT1G72050 | 50.178 | 281 | 89 | 2 | 111 | 340 | 2 | 282 | 3.55e-90 | 273 |
| MS.gene016579.t1 | AT1G72050 | 50.362 | 276 | 86 | 2 | 111 | 335 | 2 | 277 | 6.45e-89 | 270 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCTTCAAGTGTGGCTTTC+CGG | 0.178984 | 8.1:+64389455 | MS.gene016579:CDS |
| GCCTTCCCACAGCCAATTTC+CGG | 0.209602 | 8.1:-64381847 | None:intergenic |
| AACTACCAGTTTCATGTTTA+CGG | 0.285852 | 8.1:-64382207 | None:intergenic |
| CCGCACGTGTGCCCGGAAAT+TGG | 0.292837 | 8.1:+64381835 | MS.gene016579:CDS |
| GGTGTTTCATTGTGATTATA+AGG | 0.329936 | 8.1:+64382237 | MS.gene016579:CDS |
| CTTTACATGCTTACAAAGAT+TGG | 0.344658 | 8.1:-64389412 | None:intergenic |
| AGGATTTAGTGTGTGCTCTA+AGG | 0.367395 | 8.1:-64382117 | None:intergenic |
| TGATGTCTCTTAGGTAGAAA+TGG | 0.378470 | 8.1:+64380861 | MS.gene016579:intron |
| TCATCAATAGTGCAAATATA+CGG | 0.379315 | 8.1:-64381625 | None:intergenic |
| CCTTCCCACAGCCAATTTCC+GGG | 0.385128 | 8.1:-64381846 | None:intergenic |
| CTCACTTTCTGCAGTTAAGC+TGG | 0.403175 | 8.1:+64382033 | MS.gene016579:intron |
| TTGCACTATTGATGATTGTC+AGG | 0.404507 | 8.1:+64381633 | MS.gene016579:CDS |
| TGAAGGGTTTATCTTGCAAA+TGG | 0.427534 | 8.1:-64389440 | None:intergenic |
| CACCTCCGTAAACATGAAAC+TGG | 0.428491 | 8.1:+64382202 | MS.gene016579:CDS |
| TCGCGATTGCAATTCTCAAC+TGG | 0.446992 | 8.1:-64381724 | None:intergenic |
| GCTAGAGACCTCAGAATTCT+CGG | 0.448555 | 8.1:-64379620 | None:intergenic |
| CACAAAATTGAATACTTGTA+AGG | 0.451656 | 8.1:+64380921 | MS.gene016579:CDS |
| AAGTCTCACAGTTCACATAC+TGG | 0.472897 | 8.1:-64382144 | None:intergenic |
| ATCCGGAAAGCCACACTTGA+AGG | 0.475004 | 8.1:-64389457 | None:intergenic |
| TCAGAAGCCGCACGTGTGCC+CGG | 0.477093 | 8.1:+64381828 | MS.gene016579:CDS |
| GATTGTGGATGCGGTTCGAC+CGG | 0.480008 | 8.1:-64379706 | None:intergenic |
| AAGTGTGGCTTTCCGGATTG+TGG | 0.481936 | 8.1:+64389462 | MS.gene016579:CDS |
| GCGATTCTCAGACGGTGAAC+CGG | 0.492307 | 8.1:+64379765 | MS.gene016579:CDS |
| ATGTGAACTGTGAGACTTGT+GGG | 0.494220 | 8.1:+64382149 | MS.gene016579:CDS |
| GAAAATCGCATGCCACAATC+CGG | 0.501297 | 8.1:-64389474 | None:intergenic |
| TGAAAGAGATCGAGAAGCAC+AGG | 0.507704 | 8.1:+64380897 | MS.gene016579:CDS |
| GTAAAACTCTTCATACAGCC+AGG | 0.508670 | 8.1:-64382082 | None:intergenic |
| GAGCGTCGGCAGCGGAGAGG+CGG | 0.515188 | 8.1:+64379795 | MS.gene016579:CDS |
| TGGATGCGGTTCGACCGGTT+CGG | 0.515710 | 8.1:-64379701 | None:intergenic |
| GACTTTGCATGTGCTGCAAT+AGG | 0.516807 | 8.1:-64380975 | None:intergenic |
| AATAGCGGCATGGCGGATGA+TGG | 0.518294 | 8.1:+64379646 | MS.gene016579:CDS |
| GAGGTAGTGTGCTTATACCC+TGG | 0.518727 | 8.1:+64382064 | MS.gene016579:CDS |
| CGTCATCTTCTACAACATGA+AGG | 0.519530 | 8.1:+64381685 | MS.gene016579:CDS |
| AGGATTTCTTACACTTGAGA+AGG | 0.521556 | 8.1:-64382272 | None:intergenic |
| GTAGAAGATGACGATTCAAA+TGG | 0.524054 | 8.1:-64381675 | None:intergenic |
| GTGTGCCCGGAAATTGGCTG+TGG | 0.529847 | 8.1:+64381841 | MS.gene016579:CDS |
| CAATCTTTGTAAGCATGTAA+AGG | 0.534955 | 8.1:+64389413 | MS.gene016579:CDS |
| AGTTAAGCTGGAATCAATAG+AGG | 0.553788 | 8.1:+64382045 | MS.gene016579:intron |
| CTGCCGACGCTCGAAATCAC+CGG | 0.558710 | 8.1:-64379784 | None:intergenic |
| GTCATCTTCTACAACATGAA+GGG | 0.559135 | 8.1:+64381686 | MS.gene016579:CDS |
| GTGGTGCTTGATTGTGGATG+CGG | 0.559218 | 8.1:-64379715 | None:intergenic |
| TAAGTCTGAACTCGTCGAGT+GGG | 0.559588 | 8.1:-64379678 | None:intergenic |
| GAGAATTCTGAGGTCTCTAG+CGG | 0.565511 | 8.1:+64379622 | MS.gene016579:CDS |
| CGAGTTCAGACTTACCGAAC+CGG | 0.568382 | 8.1:+64379687 | MS.gene016579:CDS |
| TCAGGCAAGTTACAGAAGAA+AGG | 0.570386 | 8.1:+64381651 | MS.gene016579:CDS |
| AAACCGGTGGTGCTTGATTG+TGG | 0.571041 | 8.1:-64379721 | None:intergenic |
| GTGATTTCGAGCGTCGGCAG+CGG | 0.571716 | 8.1:+64379787 | MS.gene016579:CDS |
| CCCGGAAATTGGCTGTGGGA+AGG | 0.577142 | 8.1:+64381846 | MS.gene016579:CDS |
| ACAGTTCACATACTGGTGGC+AGG | 0.578620 | 8.1:-64382137 | None:intergenic |
| CAAGATAAACCCTTCAAGTG+TGG | 0.582886 | 8.1:+64389447 | MS.gene016579:CDS |
| TCCGGAAAGCCACACTTGAA+GGG | 0.588814 | 8.1:-64389456 | None:intergenic |
| GAGAAGCACAAACATTCTCA+TGG | 0.599567 | 8.1:+64381892 | MS.gene016579:CDS |
| AGAATTCTGAGGTCTCTAGC+GGG | 0.599974 | 8.1:+64379623 | MS.gene016579:CDS |
| GTAAGTCTGAACTCGTCGAG+TGG | 0.600296 | 8.1:-64379679 | None:intergenic |
| CCAATTTCCGGGCACACGTG+CGG | 0.601234 | 8.1:-64381835 | None:intergenic |
| TTCGAGCGTCGGCAGCGGAG+AGG | 0.603487 | 8.1:+64379792 | MS.gene016579:CDS |
| AAACATGACAAGACATGTCA+AGG | 0.604864 | 8.1:+64381762 | MS.gene016579:CDS |
| CATCCACAATCAAGCACCAC+CGG | 0.604947 | 8.1:+64379718 | MS.gene016579:CDS |
| TCATCTTCTACAACATGAAG+GGG | 0.606164 | 8.1:+64381687 | MS.gene016579:CDS |
| CTCTAGCGGGAATAGCGGCA+TGG | 0.615046 | 8.1:+64379636 | MS.gene016579:CDS |
| GGGACTAGGCGATTCTCAGA+CGG | 0.620699 | 8.1:+64379757 | MS.gene016579:CDS |
| TGTCAAGGAGTTTCATAACA+AGG | 0.621209 | 8.1:+64381777 | MS.gene016579:CDS |
| ATTGTCAGGAAACACCTCAA+GGG | 0.638939 | 8.1:-64381001 | None:intergenic |
| TAAAACTCTTCATACAGCCA+GGG | 0.647065 | 8.1:-64382081 | None:intergenic |
| AGACATGACCGAGAATTCTG+AGG | 0.648335 | 8.1:+64379612 | None:intergenic |
| TGAAACTGGTAGTTCATCAG+AGG | 0.651126 | 8.1:+64382216 | MS.gene016579:CDS |
| GAGGTCTCTAGCGGGAATAG+CGG | 0.651299 | 8.1:+64379631 | MS.gene016579:CDS |
| GAACCGGTGATTTCGAGCGT+CGG | 0.657604 | 8.1:+64379781 | MS.gene016579:CDS |
| TACCAGTTTCATGTTTACGG+AGG | 0.669845 | 8.1:-64382204 | None:intergenic |
| GAAAGAGATCGAGAAGCACA+GGG | 0.670822 | 8.1:+64380898 | MS.gene016579:CDS |
| CATGCAAAGTCATTCCCTTG+AGG | 0.672968 | 8.1:+64380987 | MS.gene016579:CDS |
| TGTGCCCGGAAATTGGCTGT+GGG | 0.690309 | 8.1:+64381842 | MS.gene016579:CDS |
| TTGAATACTTGTAAGGAGTG+TGG | 0.692585 | 8.1:+64380928 | MS.gene016579:CDS |
| TAGCGGGAATAGCGGCATGG+CGG | 0.696975 | 8.1:+64379639 | MS.gene016579:CDS |
| TCTCACAGTTCACATACTGG+TGG | 0.715768 | 8.1:-64382141 | None:intergenic |
| TATGTGAACTGTGAGACTTG+TGG | 0.750818 | 8.1:+64382148 | MS.gene016579:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 64379616 | 64390188 | 64379616 | ID=MS.gene016579 |
| chr8.1 | mRNA | 64379616 | 64390188 | 64379616 | ID=MS.gene016579.t1;Parent=MS.gene016579 |
| chr8.1 | exon | 64379616 | 64379816 | 64379616 | ID=MS.gene016579.t1.exon1;Parent=MS.gene016579.t1 |
| chr8.1 | CDS | 64379616 | 64379816 | 64379616 | ID=cds.MS.gene016579.t1;Parent=MS.gene016579.t1 |
| chr8.1 | exon | 64380874 | 64381008 | 64380874 | ID=MS.gene016579.t1.exon2;Parent=MS.gene016579.t1 |
| chr8.1 | CDS | 64380874 | 64381008 | 64380874 | ID=cds.MS.gene016579.t1;Parent=MS.gene016579.t1 |
| chr8.1 | exon | 64381622 | 64381913 | 64381622 | ID=MS.gene016579.t1.exon3;Parent=MS.gene016579.t1 |
| chr8.1 | CDS | 64381622 | 64381913 | 64381622 | ID=cds.MS.gene016579.t1;Parent=MS.gene016579.t1 |
| chr8.1 | exon | 64382047 | 64382282 | 64382047 | ID=MS.gene016579.t1.exon4;Parent=MS.gene016579.t1 |
| chr8.1 | CDS | 64382047 | 64382282 | 64382047 | ID=cds.MS.gene016579.t1;Parent=MS.gene016579.t1 |
| chr8.1 | exon | 64389408 | 64389551 | 64389408 | ID=MS.gene016579.t1.exon5;Parent=MS.gene016579.t1 |
| chr8.1 | CDS | 64389408 | 64389551 | 64389408 | ID=cds.MS.gene016579.t1;Parent=MS.gene016579.t1 |
| chr8.1 | exon | 64390174 | 64390188 | 64390174 | ID=MS.gene016579.t1.exon6;Parent=MS.gene016579.t1 |
| chr8.1 | CDS | 64390174 | 64390188 | 64390174 | ID=cds.MS.gene016579.t1;Parent=MS.gene016579.t1 |
| Gene Sequence |
| Protein sequence |
