Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016606.t1 | XP_003627845.1 | 99 | 99 | 1 | 0 | 5 | 103 | 229 | 327 | 3.00E-49 | 204.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016606.t1 | Q94CK9 | 53.7 | 82 | 35 | 2 | 9 | 90 | 220 | 298 | 1.9e-15 | 83.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016606.t1 | G7LG16 | 99.0 | 99 | 1 | 0 | 5 | 103 | 229 | 327 | 2.2e-49 | 204.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene016606.t1 | TF | SRS |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016606.t1 | MTR_8g039110 | 98.990 | 99 | 1 | 0 | 5 | 103 | 229 | 327 | 1.75e-68 | 208 |
| MS.gene016606.t1 | MTR_3g014660 | 77.320 | 97 | 18 | 4 | 7 | 102 | 235 | 328 | 2.38e-41 | 138 |
| MS.gene016606.t1 | MTR_5g089750 | 60.294 | 68 | 26 | 1 | 7 | 74 | 350 | 416 | 7.78e-22 | 88.6 |
| MS.gene016606.t1 | MTR_5g021130 | 50.000 | 52 | 26 | 0 | 9 | 60 | 202 | 253 | 1.36e-11 | 59.3 |
| MS.gene016606.t1 | MTR_8g076620 | 53.191 | 47 | 22 | 0 | 12 | 58 | 204 | 250 | 7.11e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene016606.t1 | AT5G12330 | 46.739 | 92 | 48 | 1 | 7 | 98 | 218 | 308 | 5.47e-21 | 85.1 |
| MS.gene016606.t1 | AT5G12330 | 46.739 | 92 | 48 | 1 | 7 | 98 | 218 | 308 | 5.47e-21 | 85.1 |
| MS.gene016606.t1 | AT5G12330 | 46.739 | 92 | 48 | 1 | 7 | 98 | 305 | 395 | 7.11e-21 | 85.9 |
| MS.gene016606.t1 | AT3G54430 | 56.061 | 66 | 27 | 2 | 7 | 71 | 118 | 182 | 3.49e-17 | 72.8 |
| MS.gene016606.t1 | AT1G19790 | 57.447 | 47 | 20 | 0 | 12 | 58 | 223 | 269 | 2.21e-12 | 61.6 |
| MS.gene016606.t1 | AT1G19790 | 57.447 | 47 | 20 | 0 | 12 | 58 | 223 | 269 | 2.21e-12 | 61.6 |
| MS.gene016606.t1 | AT1G75520 | 56.863 | 51 | 20 | 1 | 10 | 58 | 232 | 282 | 5.60e-12 | 60.5 |
| MS.gene016606.t1 | AT5G66350 | 48.148 | 54 | 28 | 0 | 12 | 65 | 214 | 267 | 5.66e-11 | 57.8 |
| MS.gene016606.t1 | AT5G66350 | 48.148 | 54 | 28 | 0 | 12 | 65 | 214 | 267 | 5.77e-11 | 57.8 |
| MS.gene016606.t1 | AT5G66350 | 48.148 | 54 | 28 | 0 | 12 | 65 | 214 | 267 | 6.06e-11 | 57.8 |
| MS.gene016606.t1 | AT2G18120 | 47.059 | 51 | 27 | 0 | 12 | 62 | 156 | 206 | 6.79e-11 | 57.0 |
| MS.gene016606.t1 | AT2G18120 | 47.059 | 51 | 27 | 0 | 12 | 62 | 158 | 208 | 7.44e-11 | 56.6 |
| MS.gene016606.t1 | AT3G51060 | 53.333 | 45 | 21 | 0 | 12 | 56 | 238 | 282 | 8.32e-11 | 57.4 |
Find 21 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGATGAACTTGCTTACT+TGG | 0.373159 | 8.2:-61209822 | MS.gene016606:CDS |
| TGCCAATAGCAGATACTCTA+TGG | 0.417109 | 8.2:+61209849 | None:intergenic |
| TTGGCTACTGTGAATATTAG+TGG | 0.419206 | 8.2:-61209803 | MS.gene016606:CDS |
| ATGTTCTTCCTCTGCAATTG+AGG | 0.420350 | 8.2:-61209666 | MS.gene016606:CDS |
| TGCCATAGAGTATCTGCTAT+TGG | 0.440334 | 8.2:-61209851 | MS.gene016606:CDS |
| GAATTGCAACTTGGAAGTAA+TGG | 0.469782 | 8.2:-61209710 | MS.gene016606:CDS |
| CAATCTCAACAAGCAGAAGC+AGG | 0.470698 | 8.2:+61209626 | None:intergenic |
| AAAGGGTTTCTCTATGATCA+AGG | 0.488489 | 8.2:-61209770 | MS.gene016606:CDS |
| ATTAGTGGCCATGTATTCAA+AGG | 0.512383 | 8.2:-61209788 | MS.gene016606:CDS |
| GAAGCAGGGTAAGCACTAGT+TGG | 0.512667 | 8.2:+61209641 | None:intergenic |
| CAACTTGGAAGTAATGGAAG+TGG | 0.519956 | 8.2:-61209704 | MS.gene016606:CDS |
| AGAGAAACCCTTTGAATACA+TGG | 0.531479 | 8.2:+61209780 | None:intergenic |
| TTAGTGGCCATGTATTCAAA+GGG | 0.533384 | 8.2:-61209787 | MS.gene016606:CDS |
| GGCAAAGTCTGCTTGAAACC+AGG | 0.539099 | 8.2:+61209905 | None:intergenic |
| GCAGGCGCGCGAACATAGCG+CGG | 0.551999 | 8.2:+61209884 | None:intergenic |
| CTATGGCACTTGAAAACAGC+AGG | 0.559201 | 8.2:+61209866 | None:intergenic |
| AGAGTATCTGCTATTGGCAA+TGG | 0.570693 | 8.2:-61209845 | MS.gene016606:CDS |
| TGTGTTTCAGAATTGCAACT+TGG | 0.580875 | 8.2:-61209719 | MS.gene016606:CDS |
| AATCTCAACAAGCAGAAGCA+GGG | 0.625538 | 8.2:+61209627 | None:intergenic |
| AGTTGGAACCTCAATTGCAG+AGG | 0.675832 | 8.2:+61209658 | None:intergenic |
| AGTTGCAATTCTGAAACACA+AGG | 0.685469 | 8.2:+61209722 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATTTTTTTTTTTTTTTG+GGG | - | chr8.2:61209788-61209807 | MS.gene016606:CDS | 10.0% |
| !! | AAATTTCAATAATCCAATTC+AGG | + | chr8.2:61209915-61209934 | None:intergenic | 20.0% |
| !! | ACAAACATTTCATAGTTTAA+AGG | + | chr8.2:61210222-61210241 | None:intergenic | 20.0% |
| !! | CAAACATTTCATAGTTTAAA+GGG | + | chr8.2:61210221-61210240 | None:intergenic | 20.0% |
| ! | TTGAAGGAATGTTGAAATTA+GGG | - | chr8.2:61209704-61209723 | MS.gene016606:CDS | 25.0% |
| ! | TTTGAAGGAATGTTGAAATT+AGG | - | chr8.2:61209703-61209722 | MS.gene016606:CDS | 25.0% |
| ATTCACCAAAACTGATAAAC+GGG | + | chr8.2:61209947-61209966 | None:intergenic | 30.0% | |
| CATTCACCAAAACTGATAAA+CGG | + | chr8.2:61209948-61209967 | None:intergenic | 30.0% | |
| CTAACCAATAAAGAAGAAAG+AGG | + | chr8.2:61210021-61210040 | None:intergenic | 30.0% | |
| TGAAGAAGGTTATTGTACAA+AGG | - | chr8.2:61209665-61209684 | MS.gene016606:CDS | 30.0% | |
| TGATCCTCTTTCTTCTTTAT+TGG | - | chr8.2:61210014-61210033 | MS.gene016606:intron | 30.0% | |
| ! | TCGGTTTCTTTTAGTCAATT+CGG | - | chr8.2:61209874-61209893 | MS.gene016606:CDS | 30.0% |
| !! | TTTATTGGTTAGCTTAGTAG+AGG | - | chr8.2:61210029-61210048 | MS.gene016606:intron | 30.0% |
| AAAAGAAACCGAATTGAGCA+AGG | + | chr8.2:61209866-61209885 | None:intergenic | 35.0% | |
| AAACGAAAACATGAGAACAC+AGG | + | chr8.2:61210164-61210183 | None:intergenic | 35.0% | |
| AAAGGGTTTCTCTATGATCA+AGG | - | chr8.2:61210392-61210411 | MS.gene016606:intron | 35.0% | |
| AGAGAAACCCTTTGAATACA+TGG | + | chr8.2:61210385-61210404 | None:intergenic | 35.0% | |
| ATTAGTGGCCATGTATTCAA+AGG | - | chr8.2:61210374-61210393 | MS.gene016606:intron | 35.0% | |
| GAATTGCAACTTGGAAGTAA+TGG | - | chr8.2:61210452-61210471 | MS.gene016606:intron | 35.0% | |
| TCTATCAACTCTTCCTGAAT+TGG | - | chr8.2:61209899-61209918 | MS.gene016606:CDS | 35.0% | |
| TGTGTTTCAGAATTGCAACT+TGG | - | chr8.2:61210443-61210462 | MS.gene016606:intron | 35.0% | |
| TTAGTGGCCATGTATTCAAA+GGG | - | chr8.2:61210375-61210394 | MS.gene016606:intron | 35.0% | |
| TTGGCTACTGTGAATATTAG+TGG | - | chr8.2:61210359-61210378 | MS.gene016606:intron | 35.0% | |
| TTTAGTCATGTTTAGCGCTT+GGG | - | chr8.2:61209747-61209766 | MS.gene016606:CDS | 35.0% | |
| ! | TGAAGATGAACTTGCTTACT+TGG | - | chr8.2:61210340-61210359 | MS.gene016606:intron | 35.0% |
| ! | TTTAGCCCGTTTATCAGTTT+TGG | - | chr8.2:61209939-61209958 | MS.gene016606:intron | 35.0% |
| ! | TTTTAGTCATGTTTAGCGCT+TGG | - | chr8.2:61209746-61209765 | MS.gene016606:CDS | 35.0% |
| !! | AGTTGCAATTCTGAAACACA+AGG | + | chr8.2:61210443-61210462 | None:intergenic | 35.0% |
| !! | CGTTTATCAGTTTTGGTGAA+TGG | - | chr8.2:61209946-61209965 | MS.gene016606:intron | 35.0% |
| !!! | CAAGTACGTCTTTTTTTCGA+GGG | - | chr8.2:61209641-61209660 | MS.gene016606:CDS | 35.0% |
| !!! | TAGTAGAGGAAGTTTTTAGC+AGG | - | chr8.2:61210043-61210062 | MS.gene016606:intron | 35.0% |
| !!! | TTTTTTTCGAGGGTTGAAGA+AGG | - | chr8.2:61209651-61209670 | MS.gene016606:CDS | 35.0% |
| ATAAGTTGCTCGCACTTTGA+AGG | - | chr8.2:61209688-61209707 | MS.gene016606:CDS | 40.0% | |
| ATCAGAGAGGTAGACACAAT+TGG | - | chr8.2:61210081-61210100 | MS.gene016606:intron | 40.0% | |
| ATGTTCTTCCTCTGCAATTG+AGG | - | chr8.2:61210496-61210515 | MS.gene016606:intron | 40.0% | |
| CCTCGAAAAAAAGACGTACT+TGG | + | chr8.2:61209643-61209662 | None:intergenic | 40.0% | |
| CTACCTCTCTGATTAGTACA+GGG | + | chr8.2:61210074-61210093 | None:intergenic | 40.0% | |
| TCTACCTCTCTGATTAGTAC+AGG | + | chr8.2:61210075-61210094 | None:intergenic | 40.0% | |
| TGCCAATAGCAGATACTCTA+TGG | + | chr8.2:61210316-61210335 | None:intergenic | 40.0% | |
| ! | AGAGTATCTGCTATTGGCAA+TGG | - | chr8.2:61210317-61210336 | MS.gene016606:intron | 40.0% |
| ! | CAACTTGGAAGTAATGGAAG+TGG | - | chr8.2:61210458-61210477 | MS.gene016606:intron | 40.0% |
| ! | TGCCATAGAGTATCTGCTAT+TGG | - | chr8.2:61210311-61210330 | MS.gene016606:intron | 40.0% |
| ! | TGTTTCTGAATTGCAGATCC+TGG | - | chr8.2:61210239-61210258 | MS.gene016606:intron | 40.0% |
| !! | ATCAGTTTTGGTGAATGGCA+AGG | - | chr8.2:61209951-61209970 | MS.gene016606:intron | 40.0% |
| !!! | CCAAGTACGTCTTTTTTTCG+AGG | - | chr8.2:61209640-61209659 | MS.gene016606:CDS | 40.0% |
| AGTTGGAACCTCAATTGCAG+AGG | + | chr8.2:61210507-61210526 | None:intergenic | 45.0% | |
| CTATGGCACTTGAAAACAGC+AGG | + | chr8.2:61210299-61210318 | None:intergenic | 45.0% | |
| CTTCACGTCCTTGCTCAATT+CGG | - | chr8.2:61209855-61209874 | MS.gene016606:CDS | 45.0% | |
| TCGACCTGTACTAATCAGAG+AGG | - | chr8.2:61210068-61210087 | MS.gene016606:intron | 45.0% | |
| !!! | ATAAGATTTTTTTTTTTTTT+TGG | - | chr8.2:61209786-61209805 | MS.gene016606:CDS | 5.0% |
| !!! | TAAGATTTTTTTTTTTTTTT+GGG | - | chr8.2:61209787-61209806 | MS.gene016606:CDS | 5.0% |
| GAAGCAGGGTAAGCACTAGT+TGG | + | chr8.2:61210524-61210543 | None:intergenic | 50.0% | |
| GGCAAAGTCTGCTTGAAACC+AGG | + | chr8.2:61210260-61210279 | None:intergenic | 50.0% | |
| GTGCCCTGTACTAATCAGAG+AGG | - | chr8.2:61210106-61210125 | MS.gene016606:intron | 50.0% | |
| TTGGTGAATGGCAAGGTCGT+TGG | - | chr8.2:61209958-61209977 | MS.gene016606:intron | 50.0% | |
| TGTACGCGCAGTGTCAGTCA+GGG | - | chr8.2:61209991-61210010 | MS.gene016606:intron | 55.0% | |
| TTGTACGCGCAGTGTCAGTC+AGG | - | chr8.2:61209990-61210009 | MS.gene016606:intron | 55.0% | |
| GCAGGCGCGCGAACATAGCG+CGG | + | chr8.2:61210281-61210300 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 61209631 | 61210553 | 61209631 | ID=MS.gene016606 |
| chr8.2 | mRNA | 61209631 | 61210553 | 61209631 | ID=MS.gene016606.t1;Parent=MS.gene016606 |
| chr8.2 | exon | 61210541 | 61210553 | 61210541 | ID=MS.gene016606.t1.exon1;Parent=MS.gene016606.t1 |
| chr8.2 | CDS | 61210541 | 61210553 | 61210541 | ID=cds.MS.gene016606.t1;Parent=MS.gene016606.t1 |
| chr8.2 | exon | 61209631 | 61209929 | 61209631 | ID=MS.gene016606.t1.exon2;Parent=MS.gene016606.t1 |
| chr8.2 | CDS | 61209631 | 61209929 | 61209631 | ID=cds.MS.gene016606.t1;Parent=MS.gene016606.t1 |
| Gene Sequence |
| Protein sequence |
