AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016683


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016683.t1 MTR_1g050438 62.813 320 39 5 1 240 223 542 1.22e-122 361
MS.gene016683.t1 MTR_0728s0020 62.813 320 39 5 1 240 239 558 5.63e-122 360
MS.gene016683.t1 MTR_3g029590 49.091 330 78 8 1 240 259 588 3.76e-86 270
MS.gene016683.t1 MTR_7g092070 48.494 332 76 12 1 240 259 587 3.12e-78 249
MS.gene016683.t1 MTR_3g435680 64.789 142 41 2 1 133 146 287 2.73e-60 195
MS.gene016683.t1 MTR_5g071070 45.148 237 103 6 1 212 143 377 1.15e-55 188
MS.gene016683.t1 MTR_5g071070 45.148 237 103 6 1 212 225 459 4.78e-55 187
MS.gene016683.t1 MTR_3g435590 59.712 139 54 1 1 137 233 371 8.09e-55 182
MS.gene016683.t1 MTR_5g082830 75.758 99 24 0 1 99 212 310 2.73e-53 182
MS.gene016683.t1 MTR_1g075890 74.747 99 25 0 1 99 224 322 4.18e-52 179
MS.gene016683.t1 MTR_3g070880 61.789 123 38 2 1 114 158 280 2.15e-48 167
MS.gene016683.t1 MTR_8g102100 56.410 117 51 0 2 118 77 193 4.99e-46 157
MS.gene016683.t1 MTR_6g090380 65.306 98 34 0 2 99 99 196 1.25e-45 156
MS.gene016683.t1 MTR_3g103960 50.000 136 62 2 1 135 48 178 2.40e-44 150
MS.gene016683.t1 MTR_2g090605 63.441 93 34 0 7 99 78 170 4.71e-42 148
MS.gene016683.t1 MTR_4g078660 56.122 98 43 0 2 99 56 153 1.16e-39 139
MS.gene016683.t1 MTR_3g464260 54.545 99 20 1 1 99 201 274 4.94e-31 120
MS.gene016683.t1 MTR_3g464270 40.397 151 75 3 5 140 8 158 9.06e-30 114
MS.gene016683.t1 MTR_3g464290 57.500 80 33 1 1 79 95 174 8.25e-28 110
MS.gene016683.t1 MTR_3g464280 43.434 99 22 1 1 99 183 247 2.91e-19 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016683.t1 AT5G12850 46.462 325 81 11 1 240 252 568 1.89e-76 244
MS.gene016683.t1 AT2G41900 47.516 322 76 10 1 240 256 566 7.74e-76 243
MS.gene016683.t1 AT5G58620 46.250 240 92 8 1 207 204 439 1.22e-55 187
MS.gene016683.t1 AT2G40140 57.143 140 54 1 1 134 209 348 2.04e-51 176
MS.gene016683.t1 AT2G40140 57.143 140 54 1 1 134 209 348 2.04e-51 176
MS.gene016683.t1 AT3G55980 72.727 99 27 0 1 99 206 304 1.01e-50 174
MS.gene016683.t1 AT3G55980 72.727 99 27 0 1 99 273 371 2.82e-50 174
MS.gene016683.t1 AT2G25900 64.286 98 35 0 2 99 86 183 6.13e-47 158
MS.gene016683.t1 AT2G25900 64.286 98 35 0 2 99 86 183 6.13e-47 158
MS.gene016683.t1 AT2G19810 63.265 98 36 0 2 99 74 171 1.22e-44 153
MS.gene016683.t1 AT4G29190 62.245 98 37 0 2 99 75 172 1.51e-44 153
MS.gene016683.t1 AT1G03790 65.657 99 33 1 2 99 78 176 1.88e-43 151
MS.gene016683.t1 AT5G07500 60.606 99 39 0 1 99 47 145 3.39e-41 141
MS.gene016683.t1 AT5G44260 61.616 99 37 1 2 99 56 154 5.52e-40 142

Find 51 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGTGCCTTGCCCTGAATTT+AGG 0.210124 8.1:+66279028 MS.gene016683:CDS
AAATGTTGAGCATCTGTTAC+AGG 0.268392 8.1:+66279726 MS.gene016683:CDS
GAGATTGGTTCAAAACTTCT+AGG 0.293302 8.1:-66279784 None:intergenic
TGGGACTAGTTGTGATAAAA+AGG 0.300849 8.1:+66279149 MS.gene016683:CDS
CAAGCCTCTTTCCTAAATTC+AGG 0.309020 8.1:-66279039 None:intergenic
TGCTGCTGCAATGAATCTTT+TGG 0.323237 8.1:+66279254 MS.gene016683:CDS
CTCCTCCGAGCATTCTCTCC+TGG 0.324682 8.1:-66278979 None:intergenic
TTCTCTCCTGGGTGCACATA+TGG 0.332799 8.1:-66278967 None:intergenic
AATTTAGGAAAGAGGCTTGT+AGG 0.339756 8.1:+66279043 MS.gene016683:CDS
TGGTCACATCAAAATGTTCT+AGG 0.356892 8.1:+66279376 MS.gene016683:CDS
TCCTCCGAGCATTCTCTCCT+GGG 0.362088 8.1:-66278978 None:intergenic
CATCTGTTACAGGCTTCATT+TGG 0.390176 8.1:+66279736 MS.gene016683:CDS
CTCGGAGGAGGGACCTTAGA+AGG 0.390750 8.1:+66278992 MS.gene016683:CDS
AGGGCTCAGTGGAGAGAGAT+TGG 0.407226 8.1:-66279799 None:intergenic
CTACGTTCGTTAACTAGATC+TGG 0.429336 8.1:+66279183 MS.gene016683:CDS
TCATTTGGCGTCCCTTCATT+CGG 0.450416 8.1:+66279751 MS.gene016683:CDS
TGTATGCAAGTGATGAATTT+CGG 0.450722 8.1:+66278884 MS.gene016683:CDS
AGCTGTAAGAGAACCTTCTA+AGG 0.451042 8.1:-66279005 None:intergenic
AATGTGCTGAGCCATTTAGA+TGG 0.452397 8.1:+66279466 MS.gene016683:CDS
CGAACACGACTCTGTAAAGA+TGG 0.469623 8.1:+66279129 MS.gene016683:CDS
CCCGTGCACAATCTCATGAT+TGG 0.480744 8.1:+66278935 MS.gene016683:CDS
AAGCCTCTTTCCTAAATTCA+GGG 0.483169 8.1:-66279038 None:intergenic
TTGCCCTGAATTTAGGAAAG+AGG 0.495870 8.1:+66279035 MS.gene016683:CDS
TCTCATTCTCAAGTGCTGCT+TGG 0.496358 8.1:+66279356 MS.gene016683:intron
GAGGGCATATTAGTATTTGA+AGG 0.496572 8.1:-66279697 None:intergenic
AGGAAAGAGGCTTGTAGGCA+TGG 0.515731 8.1:+66279048 MS.gene016683:CDS
CTCATGGAATATTCGAGTGT+TGG 0.522534 8.1:+66279088 MS.gene016683:CDS
ATTGTGCACGGGAACAATGA+AGG 0.527678 8.1:-66278924 None:intergenic
GGTGATGTGCTACCGAATGA+AGG 0.529639 8.1:-66279763 None:intergenic
CAGGAGAGAATGCTCGGAGG+AGG 0.532536 8.1:+66278980 MS.gene016683:CDS
CAGGCAGAATGTCGTGAGGA+TGG 0.541793 8.1:-66279435 None:intergenic
ATAGCAGCTGATTTATGTGA+AGG 0.546498 8.1:-66279649 None:intergenic
TGCACCCAGGAGAGAATGCT+CGG 0.556534 8.1:+66278974 MS.gene016683:CDS
TCCAATCATGAGATTGTGCA+CGG 0.561774 8.1:-66278936 None:intergenic
ATGTGCTGAGCCATTTAGAT+GGG 0.563955 8.1:+66279467 MS.gene016683:CDS
AGGAGAGAATGCTCGGAGGA+GGG 0.569188 8.1:+66278981 MS.gene016683:CDS
TCTTTCCTAAATTCAGGGCA+AGG 0.573700 8.1:-66279033 None:intergenic
TAGCAGCTGATTTATGTGAA+GGG 0.575464 8.1:-66279648 None:intergenic
TTGTGCACGGGAACAATGAA+GGG 0.582751 8.1:-66278923 None:intergenic
GATAAGTGTGAATATGCTCA+TGG 0.587938 8.1:+66279072 MS.gene016683:CDS
GTGATGTGCTACCGAATGAA+GGG 0.591018 8.1:-66279762 None:intergenic
AGCAGCTGATTTATGTGAAG+GGG 0.592283 8.1:-66279647 None:intergenic
CAGGTATTCTGACCCAGCAA+TGG 0.597476 8.1:+66279612 MS.gene016683:intron
GAGGCAGATGAACGTAGTGA+AGG 0.609734 8.1:-66279210 None:intergenic
GTCCATCGCATTAAAAGCAG+AGG 0.612380 8.1:-66279229 None:intergenic
GAACACGACTCTGTAAAGAT+GGG 0.635654 8.1:+66279130 MS.gene016683:CDS
AGTCGTGTTCGATACTGATG+TGG 0.638739 8.1:-66279117 None:intergenic
CCAATCATGAGATTGTGCAC+GGG 0.643224 8.1:-66278935 None:intergenic
GTCGTGTTCGATACTGATGT+GGG 0.690047 8.1:-66279116 None:intergenic
GAGTGTCCATATGTGCACCC+AGG 0.695919 8.1:+66278961 MS.gene016683:CDS
ACCCAGGAGAGAATGCTCGG+AGG 0.756453 8.1:+66278977 MS.gene016683:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTGGGAAAAATTAAATTAGA+TGG - chr8.1:66279312-66279331 None:intergenic 25.0%
ACTAAATCCATCAAATCTTG+AGG + chr8.1:66279558-66279577 MS.gene016683:intron 30.0%
TGTATGCAAGTGATGAATTT+CGG + chr8.1:66278884-66278903 MS.gene016683:CDS 30.0%
! AATTTTCAATCTAGTCGGTT+GGG + chr8.1:66279403-66279422 MS.gene016683:CDS 30.0%
! ATTTCGGATGTTTTCTTTCA+AGG + chr8.1:66278900-66278919 MS.gene016683:CDS 30.0%
! GAAGCAATTTTCAATCTAGT+CGG + chr8.1:66279398-66279417 MS.gene016683:CDS 30.0%
!!! AACAGATGCTCAACATTTTT+TGG - chr8.1:66279724-66279743 None:intergenic 30.0%
AAAAAACAGAAGCCATTGCT+GGG - chr8.1:66279627-66279646 None:intergenic 35.0%
AAATGTTGAGCATCTGTTAC+AGG + chr8.1:66279726-66279745 MS.gene016683:CDS 35.0%
AACAGCTCAAGTCATTCATA+AGG - chr8.1:66279501-66279520 None:intergenic 35.0%
AAGAGATCCTCAAGATTTGA+TGG - chr8.1:66279568-66279587 None:intergenic 35.0%
AAGCCTCTTTCCTAAATTCA+GGG - chr8.1:66279041-66279060 None:intergenic 35.0%
AATTTAGGAAAGAGGCTTGT+AGG + chr8.1:66279043-66279062 MS.gene016683:CDS 35.0%
GATAAGTGTGAATATGCTCA+TGG + chr8.1:66279072-66279091 MS.gene016683:CDS 35.0%
TGGTCACATCAAAATGTTCT+AGG + chr8.1:66279376-66279395 MS.gene016683:CDS 35.0%
! ATAGCAGCTGATTTATGTGA+AGG - chr8.1:66279652-66279671 None:intergenic 35.0%
! CAATTTTCAATCTAGTCGGT+TGG + chr8.1:66279402-66279421 MS.gene016683:CDS 35.0%
! GAGATTGGTTCAAAACTTCT+AGG - chr8.1:66279787-66279806 None:intergenic 35.0%
! GAGGGCATATTAGTATTTGA+AGG - chr8.1:66279700-66279719 None:intergenic 35.0%
! GGTTTTTCTACCCATCTAAA+TGG - chr8.1:66279480-66279499 None:intergenic 35.0%
! TAGCAGCTGATTTATGTGAA+GGG - chr8.1:66279651-66279670 None:intergenic 35.0%
!! TGGGACTAGTTGTGATAAAA+AGG + chr8.1:66279149-66279168 MS.gene016683:CDS 35.0%
!!! ATGCTCAACATTTTTTGGAG+AGG - chr8.1:66279719-66279738 None:intergenic 35.0%
!!! TGCTCAACATTTTTTGGAGA+GGG - chr8.1:66279718-66279737 None:intergenic 35.0%
AATGTGCTGAGCCATTTAGA+TGG + chr8.1:66279466-66279485 MS.gene016683:CDS 40.0%
AGCTGTAAGAGAACCTTCTA+AGG - chr8.1:66279008-66279027 None:intergenic 40.0%
ATGAACGAAAGAGACTAACG+AGG - chr8.1:66279289-66279308 None:intergenic 40.0%
ATGTGCTGAGCCATTTAGAT+GGG + chr8.1:66279467-66279486 MS.gene016683:CDS 40.0%
CAAGCCTCTTTCCTAAATTC+AGG - chr8.1:66279042-66279061 None:intergenic 40.0%
CATCTGTTACAGGCTTCATT+TGG + chr8.1:66279736-66279755 MS.gene016683:CDS 40.0%
CTACGTTCGTTAACTAGATC+TGG + chr8.1:66279183-66279202 MS.gene016683:CDS 40.0%
CTCATGGAATATTCGAGTGT+TGG + chr8.1:66279088-66279107 MS.gene016683:CDS 40.0%
GAAAAAACAGAAGCCATTGC+TGG - chr8.1:66279628-66279647 None:intergenic 40.0%
GAACACGACTCTGTAAAGAT+GGG + chr8.1:66279130-66279149 MS.gene016683:CDS 40.0%
TCCAATCATGAGATTGTGCA+CGG - chr8.1:66278939-66278958 None:intergenic 40.0%
TCTTTCCTAAATTCAGGGCA+AGG - chr8.1:66279036-66279055 None:intergenic 40.0%
TGAACGAAAGAGACTAACGA+GGG - chr8.1:66279288-66279307 None:intergenic 40.0%
TTGCCCTGAATTTAGGAAAG+AGG + chr8.1:66279035-66279054 MS.gene016683:CDS 40.0%
! AATGAGATGCCATTTCCAGA+AGG - chr8.1:66279343-66279362 None:intergenic 40.0%
! AGCAGCTGATTTATGTGAAG+GGG - chr8.1:66279650-66279669 None:intergenic 40.0%
! ATGAGATGCCATTTCCAGAA+GGG - chr8.1:66279342-66279361 None:intergenic 40.0%
! CTCAGCACATTGAAGTTTTC+AGG - chr8.1:66279457-66279476 None:intergenic 40.0%
! GCTGCAATGAATCTTTTGGT+CGG + chr8.1:66279258-66279277 MS.gene016683:CDS 40.0%
! TCTTGAGGATCTCTTTTGTG+CGG + chr8.1:66279573-66279592 MS.gene016683:intron 40.0%
! TGCTGCTGCAATGAATCTTT+TGG + chr8.1:66279254-66279273 MS.gene016683:CDS 40.0%
AGTCGTGTTCGATACTGATG+TGG - chr8.1:66279120-66279139 None:intergenic 45.0%
ATTGCTGGGTCAGAATACCT+GGG - chr8.1:66279613-66279632 None:intergenic 45.0%
ATTGTGCACGGGAACAATGA+AGG - chr8.1:66278927-66278946 None:intergenic 45.0%
CCAATCATGAGATTGTGCAC+GGG - chr8.1:66278938-66278957 None:intergenic 45.0%
CGAACACGACTCTGTAAAGA+TGG + chr8.1:66279129-66279148 MS.gene016683:CDS 45.0%
GTCCATCGCATTAAAAGCAG+AGG - chr8.1:66279232-66279251 None:intergenic 45.0%
GTCGTGTTCGATACTGATGT+GGG - chr8.1:66279119-66279138 None:intergenic 45.0%
GTGATGTGCTACCGAATGAA+GGG - chr8.1:66279765-66279784 None:intergenic 45.0%
TCATTTGGCGTCCCTTCATT+CGG + chr8.1:66279751-66279770 MS.gene016683:CDS 45.0%
TTGTGCACGGGAACAATGAA+GGG - chr8.1:66278926-66278945 None:intergenic 45.0%
! CGGAAATTTTGTCATCTCCC+AGG + chr8.1:66279593-66279612 MS.gene016683:intron 45.0%
! TCTCATTCTCAAGTGCTGCT+TGG + chr8.1:66279356-66279375 MS.gene016683:intron 45.0%
! TGAGATGCCATTTCCAGAAG+GGG - chr8.1:66279341-66279360 None:intergenic 45.0%
!! AACCCAGTCCTAGTTCTAGT+CGG + chr8.1:66279527-66279546 MS.gene016683:intron 45.0%
!! TCCTAGTTCTAGTCGGTCTA+AGG + chr8.1:66279534-66279553 MS.gene016683:intron 45.0%
!! TGCCTCTGCTTTTAATGCGA+TGG + chr8.1:66279227-66279246 MS.gene016683:CDS 45.0%
!! TTTTCAGGCAGAATGTCGTG+AGG - chr8.1:66279442-66279461 None:intergenic 45.0%
AGACCGACTAGAACTAGGAC+TGG - chr8.1:66279533-66279552 None:intergenic 50.0%
AGGAAAGAGGCTTGTAGGCA+TGG + chr8.1:66279048-66279067 MS.gene016683:CDS 50.0%
CAGGTATTCTGACCCAGCAA+TGG + chr8.1:66279612-66279631 MS.gene016683:intron 50.0%
CATTGCTGGGTCAGAATACC+TGG - chr8.1:66279614-66279633 None:intergenic 50.0%
CCCGTGCACAATCTCATGAT+TGG + chr8.1:66278935-66278954 MS.gene016683:CDS 50.0%
GACCGACTAGAACTAGGACT+GGG - chr8.1:66279532-66279551 None:intergenic 50.0%
GAGGCAGATGAACGTAGTGA+AGG - chr8.1:66279213-66279232 None:intergenic 50.0%
GCCTTAGACCGACTAGAACT+AGG - chr8.1:66279538-66279557 None:intergenic 50.0%
GGTGATGTGCTACCGAATGA+AGG - chr8.1:66279766-66279785 None:intergenic 50.0%
TTCTCTCCTGGGTGCACATA+TGG - chr8.1:66278970-66278989 None:intergenic 50.0%
AGGAGAGAATGCTCGGAGGA+GGG + chr8.1:66278981-66279000 MS.gene016683:CDS 55.0%
CAGGCAGAATGTCGTGAGGA+TGG - chr8.1:66279438-66279457 None:intergenic 55.0%
GAGTGTCCATATGTGCACCC+AGG + chr8.1:66278961-66278980 MS.gene016683:CDS 55.0%
GCGTGCCTTGCCCTGAATTT+AGG + chr8.1:66279028-66279047 MS.gene016683:CDS 55.0%
TCCTCCGAGCATTCTCTCCT+GGG - chr8.1:66278981-66279000 None:intergenic 55.0%
TGCACCCAGGAGAGAATGCT+CGG + chr8.1:66278974-66278993 MS.gene016683:CDS 55.0%
! GCCATTTCCAGAAGGGGCAT+AGG - chr8.1:66279335-66279354 None:intergenic 55.0%
! GCCTATGCCCCTTCTGGAAA+TGG + chr8.1:66279331-66279350 MS.gene016683:intron 55.0%
! TTCCAGAAGGGGCATAGGCT+GGG - chr8.1:66279330-66279349 None:intergenic 55.0%
! TTTCCAGAAGGGGCATAGGC+TGG - chr8.1:66279331-66279350 None:intergenic 55.0%
ACCCAGGAGAGAATGCTCGG+AGG + chr8.1:66278977-66278996 MS.gene016683:CDS 60.0%
CAGGAGAGAATGCTCGGAGG+AGG + chr8.1:66278980-66278999 MS.gene016683:CDS 60.0%
CTCCTCCGAGCATTCTCTCC+TGG - chr8.1:66278982-66279001 None:intergenic 60.0%
CTCGGAGGAGGGACCTTAGA+AGG + chr8.1:66278992-66279011 MS.gene016683:CDS 60.0%
TTCCCAGCCTATGCCCCTTC+TGG + chr8.1:66279325-66279344 MS.gene016683:intron 60.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 66278883 66279816 66278883 ID=MS.gene016683
chr8.1 mRNA 66278883 66279816 66278883 ID=MS.gene016683.t1;Parent=MS.gene016683
chr8.1 exon 66278883 66279289 66278883 ID=MS.gene016683.t1.exon1;Parent=MS.gene016683.t1
chr8.1 CDS 66278883 66279289 66278883 ID=cds.MS.gene016683.t1;Parent=MS.gene016683.t1
chr8.1 exon 66279369 66279488 66279369 ID=MS.gene016683.t1.exon2;Parent=MS.gene016683.t1
chr8.1 CDS 66279369 66279488 66279369 ID=cds.MS.gene016683.t1;Parent=MS.gene016683.t1
chr8.1 exon 66279615 66279816 66279615 ID=MS.gene016683.t1.exon3;Parent=MS.gene016683.t1
chr8.1 CDS 66279615 66279816 66279615 ID=cds.MS.gene016683.t1;Parent=MS.gene016683.t1
Gene Sequence

>MS.gene016683

ATGTATGCAAGTGATGAATTTCGGATGTTTTCTTTCAAGGCCCTTCATTGTTCCCGTGCACAATCTCATGATTGGACTGAGTGTCCATATGTGCACCCAGGAGAGAATGCTCGGAGGAGGGACCTTAGAAGGTTCTCTTACAGCTGCGTGCCTTGCCCTGAATTTAGGAAAGAGGCTTGTAGGCATGGAGATAAGTGTGAATATGCTCATGGAATATTCGAGTGTTGGCTACACCCACATCAGTATCGAACACGACTCTGTAAAGATGGGACTAGTTGTGATAAAAAGGTGTGTTTTTCACTACGTTCGTTAACTAGATCTGGTGTTCCTTCACTACGTTCATCTGCCTCTGCTTTTAATGCGATGGACATTGCTGCTGCAATGAATCTTTTGGTCGGTTCACCCTCGTTAGTCTCTTTCGTTCATCCATCTAATTTAATTTTTCCCAGCCTATGCCCCTTCTGGAAATGGCATCTCATTCTCAAGTGCTGCTTGGTCACATCAAAATGTTCTAGGAAGCAATTTTCAATCTAGTCGGTTGGGATCTTCTCTCCATCCTCACGACATTCTGCCTGAAAACTTCAATGTGCTGAGCCATTTAGATGGGTAGAAAAACCTTATGAATGACTTGAGCTGTTTCTCAAAACCCAGTCCTAGTTCTAGTCGGTCTAAGGCACTAAATCCATCAAATCTTGAGGATCTCTTTTGTGCGGAAATTTTGTCATCTCCCAGGTATTCTGACCCAGCAATGGCTTCTGTTTTTTCCCCTTCACATAAATCAGCTGCTATGAATGAGCTTCAAAACATGTTATCGCCTTCAAATACTAATATGCCCTCTCCAAAAAATGTTGAGCATCTGTTACAGGCTTCATTTGGCGTCCCTTCATTCGGTAGCACATCACCTAGAAGTTTTGAACCAATCTCTCTCCACTGA

Protein sequence

>MS.gene016683.t1

MYASDEFRMFSFKALHCSRAQSHDWTECPYVHPGENARRRDLRRFSYSCVPCPEFRKEACRHGDKCEYAHGIFECWLHPHQYRTRLCKDGTSCDKKVCFSLRSLTRSGVPSLRSSASAFNAMDIAAAMNLLVGSPSAAWSHQNVLGSNFQSSRLGSSLHPHDILPENFNVLSHLDGYSDPAMASVFSPSHKSAAMNELQNMLSPSNTNMPSPKNVEHLLQASFGVPSFGSTSPRSFEPISLH