AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016820.t1 MTR_5g086960 74.866 187 22 1 6 192 22 183 5.63e-96 276
MS.gene016820.t1 MTR_5g086940 74.332 187 23 1 6 192 151 312 1.58e-94 278
MS.gene016820.t1 MTR_5g087050 76.216 185 19 1 6 190 412 571 3.71e-94 286
MS.gene016820.t1 MTR_5g086790 76.216 185 19 1 6 190 480 639 9.20e-94 286
MS.gene016820.t1 MTR_5g087240 75.135 185 21 1 6 190 424 583 2.11e-91 281
MS.gene016820.t1 MTR_5g086970 70.588 187 30 1 6 192 249 410 2.18e-88 265
MS.gene016820.t1 MTR_5g087250 71.351 185 28 1 6 190 317 476 1.25e-87 266
MS.gene016820.t1 MTR_3g045010 77.419 124 21 1 6 122 68 191 1.12e-63 196
MS.gene016820.t1 MTR_4g125800 28.571 154 78 4 11 164 103 224 9.36e-15 71.2
MS.gene016820.t1 MTR_8g015150 36.207 116 69 3 1 115 391 502 1.07e-14 72.0
MS.gene016820.t1 MTR_8g015150 36.207 116 69 3 1 115 636 747 1.14e-14 72.0
MS.gene016820.t1 MTR_4g125800 28.571 154 78 4 11 164 156 277 1.35e-14 71.2
MS.gene016820.t1 MTR_4g125800 28.571 154 78 4 11 164 179 300 2.21e-14 70.5
MS.gene016820.t1 MTR_2g040510 31.373 153 67 5 8 156 153 271 3.17e-14 70.1
MS.gene016820.t1 MTR_7g015420 34.028 144 58 4 26 165 625 735 3.86e-14 70.5
MS.gene016820.t1 MTR_8g028115 38.235 102 58 3 15 115 12 109 4.26e-14 68.6
MS.gene016820.t1 MTR_1g104555 29.814 161 79 5 20 179 481 608 7.80e-14 69.3
MS.gene016820.t1 MTR_6g090470 28.302 159 80 5 5 163 149 273 1.21e-13 68.6
MS.gene016820.t1 MTR_2g099530 27.329 161 87 4 15 173 397 529 1.41e-13 68.6
MS.gene016820.t1 MTR_8g028110 38.235 102 58 3 15 115 662 759 3.33e-13 67.8
MS.gene016820.t1 MTR_8g014760 36.792 106 62 3 11 115 648 749 4.34e-13 67.4
MS.gene016820.t1 MTR_8g015040 35.345 116 70 3 1 115 693 804 5.01e-13 67.0
MS.gene016820.t1 MTR_2g105930 30.882 136 59 5 26 157 476 580 5.05e-13 67.0
MS.gene016820.t1 MTR_8g014930 33.621 116 72 3 1 115 641 752 5.41e-13 67.0
MS.gene016820.t1 MTR_8g014930 33.621 116 72 3 1 115 607 718 5.64e-13 67.0
MS.gene016820.t1 MTR_2g105930 30.882 136 59 5 26 157 560 664 5.96e-13 67.0
MS.gene016820.t1 MTR_8g015190 38.000 100 57 3 17 115 693 788 7.04e-13 66.6
MS.gene016820.t1 MTR_8g015190 38.000 100 57 3 17 115 683 778 7.60e-13 66.6
MS.gene016820.t1 MTR_8g014970 35.345 116 70 3 1 115 407 518 7.74e-13 66.6
MS.gene016820.t1 MTR_2g020380 29.936 157 80 4 7 162 82 209 8.54e-13 66.6
MS.gene016820.t1 MTR_4g087620 28.144 167 81 7 7 170 142 272 9.11e-13 65.5
MS.gene016820.t1 MTR_3g104900 29.412 153 72 6 4 156 262 378 1.02e-12 65.9
MS.gene016820.t1 MTR_8g014860 44.872 78 39 2 21 97 195 269 1.03e-12 65.5
MS.gene016820.t1 MTR_4g087620 28.144 167 82 6 7 171 142 272 1.54e-12 65.5
MS.gene016820.t1 MTR_8g086000 33.557 149 64 7 7 155 162 275 1.58e-12 65.5
MS.gene016820.t1 MTR_8g086000 33.557 149 64 7 7 155 119 232 1.58e-12 65.5
MS.gene016820.t1 MTR_8g014700 35.345 116 70 3 1 115 647 758 1.87e-12 65.5
MS.gene016820.t1 MTR_8g014740 28.369 141 69 4 26 165 29 138 1.88e-12 63.9
MS.gene016820.t1 MTR_1g105595 40.000 100 55 3 17 115 440 535 2.13e-12 65.1
MS.gene016820.t1 MTR_1g105595 43.373 83 43 2 17 98 393 472 2.28e-12 65.1
MS.gene016820.t1 MTR_6g088610 30.864 162 75 6 13 173 383 508 2.53e-12 65.1
MS.gene016820.t1 MTR_1g019340 28.079 203 113 8 7 190 192 380 2.81e-12 64.7
MS.gene016820.t1 MTR_3g079850 44.318 88 38 3 19 98 429 513 2.98e-12 64.7
MS.gene016820.t1 MTR_8g015200 34.483 116 71 3 1 115 618 729 3.79e-12 64.7
MS.gene016820.t1 MTR_1g019340 28.079 203 113 8 7 190 192 380 4.15e-12 64.3
MS.gene016820.t1 MTR_8g101320 29.252 147 68 5 16 157 532 647 4.30e-12 64.3
MS.gene016820.t1 MTR_2g017825 38.614 101 57 3 5 104 232 328 5.36e-12 63.9
MS.gene016820.t1 MTR_8g014700 41.860 86 45 3 31 115 701 782 5.39e-12 64.3
MS.gene016820.t1 MTR_8g014790 35.849 106 63 3 11 115 653 754 5.52e-12 63.9
MS.gene016820.t1 MTR_2g017825 38.614 101 57 3 5 104 232 328 6.02e-12 63.5
MS.gene016820.t1 MTR_5g058090 40.385 104 58 3 13 115 673 773 7.03e-12 63.9
MS.gene016820.t1 MTR_8g040300 28.324 173 78 6 20 190 107 235 7.34e-12 62.8
MS.gene016820.t1 MTR_2g017825 31.646 158 75 5 7 163 234 359 7.72e-12 63.5
MS.gene016820.t1 MTR_2g017825 31.646 158 75 5 7 163 230 355 7.77e-12 63.5
MS.gene016820.t1 MTR_8g015100 39.560 91 50 3 26 115 364 450 8.51e-12 63.5
MS.gene016820.t1 MTR_8g015100 39.560 91 50 3 26 115 581 667 1.10e-11 63.2
MS.gene016820.t1 MTR_8g014690 38.776 98 56 2 1 97 645 739 1.12e-11 63.2
MS.gene016820.t1 MTR_7g056420 39.175 97 52 3 6 98 920 1013 1.22e-11 63.2
MS.gene016820.t1 MTR_7g056430 32.026 153 69 5 6 154 920 1041 1.25e-11 63.2
MS.gene016820.t1 MTR_5g005480 42.268 97 50 4 6 98 409 503 1.27e-11 63.2
MS.gene016820.t1 MTR_2g067970 29.762 168 81 7 15 178 695 829 1.91e-11 62.4
MS.gene016820.t1 MTR_8g101300 28.571 147 69 5 16 157 560 675 2.02e-11 62.4
MS.gene016820.t1 MTR_5g067150 30.935 139 62 4 18 156 104 208 2.06e-11 62.4
MS.gene016820.t1 MTR_5g067150 30.935 139 62 4 18 156 104 208 2.11e-11 62.0
MS.gene016820.t1 MTR_5g067150 30.935 139 62 4 18 156 104 208 2.26e-11 62.0
MS.gene016820.t1 MTR_6g055960 45.833 72 31 3 30 98 702 768 2.43e-11 62.4
MS.gene016820.t1 MTR_3g072990 32.331 133 65 6 29 161 495 602 2.43e-11 62.0
MS.gene016820.t1 MTR_3g072990 32.331 133 65 6 29 161 474 581 2.51e-11 62.0
MS.gene016820.t1 MTR_3g072990 32.331 133 65 6 29 161 497 604 2.57e-11 62.0
MS.gene016820.t1 MTR_0007s0020 44.444 72 32 3 30 98 680 746 2.58e-11 62.0
MS.gene016820.t1 MTR_5g068290 36.111 108 63 4 22 126 251 355 2.65e-11 61.6
MS.gene016820.t1 MTR_3g072990 32.331 133 65 6 29 161 472 579 2.66e-11 62.0
MS.gene016820.t1 MTR_0074s0060 42.105 76 36 3 26 98 412 482 2.69e-11 62.0
MS.gene016820.t1 MTR_8g013580 32.143 140 62 5 30 167 616 724 3.56e-11 61.6
MS.gene016820.t1 MTR_6g071280 28.205 156 80 5 1 156 136 259 4.10e-11 61.2
MS.gene016820.t1 MTR_4g040480 44.286 70 35 2 30 98 618 684 5.15e-11 61.2
MS.gene016820.t1 MTR_2g014960 33.918 171 90 7 32 188 718 879 5.20e-11 61.2
MS.gene016820.t1 MTR_4g069970 29.496 139 65 4 32 169 249 355 5.32e-11 60.8
MS.gene016820.t1 MTR_8g461120 35.849 106 63 3 11 115 640 741 5.72e-11 61.2
MS.gene016820.t1 MTR_8g461120 37.000 100 58 3 17 115 671 766 5.86e-11 61.2
MS.gene016820.t1 MTR_4g069970 29.496 139 65 4 32 169 249 355 6.30e-11 60.8
MS.gene016820.t1 MTR_5g068250 44.286 70 35 2 30 98 451 517 6.46e-11 60.8
MS.gene016820.t1 MTR_1g029610 34.677 124 71 5 22 141 391 508 6.63e-11 60.8
MS.gene016820.t1 MTR_2g017825 44.444 72 39 1 5 75 232 303 6.76e-11 60.5
MS.gene016820.t1 MTR_5g086040 31.788 151 68 5 32 179 432 550 7.51e-11 60.8
MS.gene016820.t1 MTR_4g069970 30.000 130 58 4 32 160 249 346 8.09e-11 60.5
MS.gene016820.t1 MTR_1g009310 30.973 113 74 4 26 136 567 677 9.98e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016820.t1 AT5G20930 68.817 186 32 2 6 190 497 657 5.85e-84 261
MS.gene016820.t1 AT4G26070 30.201 149 70 4 8 156 150 264 8.63e-14 68.9
MS.gene016820.t1 AT4G26070 30.201 149 70 4 8 156 151 265 1.04e-13 68.6
MS.gene016820.t1 AT4G26070 30.201 149 70 4 8 156 151 265 1.04e-13 68.6
MS.gene016820.t1 AT4G26070 30.201 149 70 4 8 156 151 265 1.07e-13 68.6
MS.gene016820.t1 AT4G26070 30.201 149 70 4 8 156 138 252 1.14e-13 68.6
MS.gene016820.t1 AT4G29810 27.632 152 76 4 12 163 166 283 2.19e-13 67.8
MS.gene016820.t1 AT4G29810 27.632 152 76 4 12 163 166 283 2.98e-13 67.4
MS.gene016820.t1 AT4G29810 27.632 152 76 4 12 163 157 274 3.01e-13 67.4
MS.gene016820.t1 AT1G70130 29.240 171 86 4 5 172 413 551 5.27e-13 67.0
MS.gene016820.t1 AT5G16000 30.952 168 80 6 12 176 398 532 7.03e-13 66.6
MS.gene016820.t1 AT1G10210 30.667 150 69 7 7 156 119 233 1.71e-12 65.1
MS.gene016820.t1 AT1G10210 30.667 150 69 7 7 156 119 233 1.71e-12 65.1
MS.gene016820.t1 AT1G10210 30.667 150 69 7 7 156 119 233 1.71e-12 65.1
MS.gene016820.t1 AT2G43790 28.144 167 82 6 7 171 150 280 1.77e-12 65.5
MS.gene016820.t1 AT1G48490 28.495 186 116 8 7 190 913 1083 2.07e-12 65.5
MS.gene016820.t1 AT1G48490 28.495 186 116 8 7 190 912 1082 2.09e-12 65.5
MS.gene016820.t1 AT1G48490 28.495 186 116 8 7 190 912 1082 2.09e-12 65.5
MS.gene016820.t1 AT1G48490 28.495 186 116 8 7 190 912 1082 2.09e-12 65.5
MS.gene016820.t1 AT3G45640 28.415 183 84 7 7 187 125 262 2.40e-12 64.7
MS.gene016820.t1 AT2G18170 31.126 151 67 7 7 156 119 233 2.65e-12 64.7
MS.gene016820.t1 AT1G77720 33.333 129 61 6 29 157 534 637 3.26e-12 64.7
MS.gene016820.t1 AT1G59580 30.201 149 69 7 7 155 119 232 3.34e-12 64.3
MS.gene016820.t1 AT1G59580 30.201 149 69 7 7 155 119 232 3.34e-12 64.3
MS.gene016820.t1 AT1G07880 27.848 158 78 5 7 163 120 242 4.04e-12 63.5
MS.gene016820.t1 AT5G56580 27.044 159 82 4 5 163 151 275 4.32e-12 63.9
MS.gene016820.t1 AT5G65240 31.707 164 77 6 16 176 378 509 5.29e-12 64.3
MS.gene016820.t1 AT5G65240 31.707 164 77 6 16 176 378 509 5.33e-12 63.9
MS.gene016820.t1 AT4G00960 47.143 70 35 2 30 98 162 230 9.77e-12 62.4
MS.gene016820.t1 AT1G01540 28.276 145 71 4 26 169 257 369 1.02e-11 63.2
MS.gene016820.t1 AT1G07880 27.848 158 78 5 7 163 120 242 1.14e-11 62.8
MS.gene016820.t1 AT3G21220 28.070 171 93 4 12 182 153 293 1.19e-11 62.8
MS.gene016820.t1 AT3G21220 28.070 171 93 4 12 182 153 293 1.19e-11 62.8
MS.gene016820.t1 AT1G16760 27.891 147 77 3 26 171 549 667 1.32e-11 63.2
MS.gene016820.t1 AT1G01540 27.660 141 69 4 26 165 257 365 1.41e-11 62.8
MS.gene016820.t1 AT1G16760 27.891 147 77 3 26 171 549 667 1.49e-11 62.8
MS.gene016820.t1 AT4G00960 39.560 91 53 2 9 98 47 136 1.66e-11 62.0
MS.gene016820.t1 AT4G00960 47.143 70 35 2 30 98 75 143 1.82e-11 62.0
MS.gene016820.t1 AT4G11330 27.545 167 83 6 7 171 130 260 2.23e-11 62.0
MS.gene016820.t1 AT4G00960 47.143 70 35 2 30 98 162 230 2.28e-11 62.0
MS.gene016820.t1 AT4G00970 41.892 74 41 2 26 98 446 518 2.49e-11 62.0
MS.gene016820.t1 AT4G00970 41.892 74 41 2 26 98 446 518 2.64e-11 62.0
MS.gene016820.t1 AT3G20200 28.873 142 72 3 26 166 552 665 5.39e-11 61.2
MS.gene016820.t1 AT3G20200 28.873 142 72 3 26 166 552 665 5.47e-11 61.2
MS.gene016820.t1 AT3G20200 28.873 142 72 3 26 166 552 665 5.47e-11 61.2
MS.gene016820.t1 AT3G20200 28.873 142 72 3 26 166 552 665 6.51e-11 60.8
MS.gene016820.t1 AT4G03230 28.671 143 70 4 26 167 692 803 7.93e-11 60.8
MS.gene016820.t1 AT4G03230 28.671 143 70 4 26 167 692 803 7.93e-11 60.8
MS.gene016820.t1 AT4G03230 28.671 143 70 4 26 167 720 831 8.27e-11 60.8
MS.gene016820.t1 AT4G03230 28.671 143 70 4 26 167 627 738 8.30e-11 60.8
MS.gene016820.t1 AT4G03230 28.671 143 70 4 26 167 635 746 8.63e-11 60.8

Find 39 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATCATAATGGATAATCTT+TGG 0.279048 5.2:-73894589 None:intergenic
CTCGGAAAATCTGGACTAAA+AGG 0.282592 5.2:-73894540 None:intergenic
TTTGCGAAAGTGACTGATTT+TGG 0.286870 5.2:+73894646 MS.gene016820:CDS
TCTTGAGGACACGATTATTA+AGG 0.299436 5.2:+73895230 MS.gene016820:CDS
TTGCAGGCTTTAAATCATAA+TGG 0.328619 5.2:-73894600 None:intergenic
TCATCAAACAAAACATTTGC+AGG 0.350348 5.2:-73894616 None:intergenic
TTGCTGCGTTCAAAGCATTC+AGG 0.364136 5.2:-73894899 None:intergenic
AAACAAGACCTCGGAAAATC+TGG 0.371570 5.2:-73894549 None:intergenic
CCCAGGGTGCTGGTACATAT+TGG 0.394551 5.2:+73894722 MS.gene016820:CDS
CTATTTGGCAGACGTCCTTT+CGG 0.417019 5.2:+73895180 MS.gene016820:CDS
GGCGAATGATGTGGGATCCC+AGG 0.436384 5.2:+73894684 MS.gene016820:CDS
GCCTCGCTCTCTGGCAATAT+AGG 0.455416 5.2:-73894511 None:intergenic
AGGACTTTAGCCTCGCTCTC+TGG 0.477673 5.2:-73894520 None:intergenic
TTAGAAACAGTAGGTGTTGA+GGG 0.477933 5.2:-73895270 None:intergenic
TGATGTGGGATCCCAGGGTA+TGG 0.489041 5.2:+73894690 MS.gene016820:CDS
ACCAATATGTACCAGCACCC+TGG 0.500239 5.2:-73894723 None:intergenic
CCAATATGTACCAGCACCCT+GGG 0.505001 5.2:-73894722 None:intergenic
GGGTATGGAACTTACATCCC+AGG 0.526804 5.2:+73894705 MS.gene016820:CDS
TTACATAAAGCATGCAAAGC+GGG 0.533025 5.2:+73894078 None:intergenic
GAACTTACATCCCAGGGTGC+TGG 0.546628 5.2:+73894712 MS.gene016820:CDS
GGATGTAAGTTCCATACCCT+GGG 0.549900 5.2:-73894701 None:intergenic
AAGATAGTGGCGAATGATGT+GGG 0.553374 5.2:+73894676 MS.gene016820:CDS
CAAGATAGTGGCGAATGATG+TGG 0.561932 5.2:+73894675 MS.gene016820:CDS
GGGATGTAAGTTCCATACCC+TGG 0.566104 5.2:-73894702 None:intergenic
GCGAATGATGTGGGATCCCA+GGG 0.570226 5.2:+73894685 MS.gene016820:CDS
ATTAGAAACAGTAGGTGTTG+AGG 0.574283 5.2:-73895271 None:intergenic
AACACCTACTGTTTCTAATG+AGG 0.586782 5.2:+73895275 MS.gene016820:CDS
CAGGTTGATGTGTGGTCTGT+CGG 0.587890 5.2:+73895138 MS.gene016820:intron
GCCTATATTGCCAGAGAGCG+AGG 0.594842 5.2:+73894510 MS.gene016820:CDS
CATTAATGTAAACAAGACCT+CGG 0.601584 5.2:-73894558 None:intergenic
GACGCCTCTCATATCATCCA+AGG 0.602900 5.2:+73894922 MS.gene016820:CDS
ACAAGAGAAAATACTTCTTG+AGG 0.612477 5.2:+73895215 MS.gene016820:CDS
TGTGTTTGGTCATGTCCGAA+AGG 0.620138 5.2:-73895195 None:intergenic
CTGCGTTCAAAGCATTCAGG+AGG 0.628821 5.2:-73894896 None:intergenic
TTTGCCTCATTAGAAACAGT+AGG 0.643783 5.2:-73895279 None:intergenic
GGTATGGAACTTACATCCCA+GGG 0.647817 5.2:+73894706 MS.gene016820:CDS
TAGACCTTGGATGATATGAG+AGG 0.670141 5.2:-73894926 None:intergenic
ATTACATAAAGCATGCAAAG+CGG 0.727091 5.2:+73894077 None:intergenic
GTGGAAAACAGGTTGATGTG+TGG 0.729884 5.2:+73895130 MS.gene016820:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTATTTAAGCTTATATAT+TGG + chr5.2:73894280-73894299 MS.gene016820:intron 15.0%
!! ACTTAAAAAATTATAGACCT+TGG - chr5.2:73894942-73894961 None:intergenic 20.0%
!!! AAATCATAATGGATAATCTT+TGG - chr5.2:73894592-73894611 None:intergenic 20.0%
!!! ATACATAGATAGCTTTTTTT+TGG + chr5.2:73894967-73894986 MS.gene016820:CDS 20.0%
!!! ATTATTTTCATAAACAGATG+AGG - chr5.2:73894218-73894237 None:intergenic 20.0%
!!! GGTTACAATTTTCAATTTTT+CGG + chr5.2:73894988-73895007 MS.gene016820:CDS 20.0%
!!! TCTTTACTATTCTTTAACTT+AGG + chr5.2:73894819-73894838 MS.gene016820:intron 20.0%
!!! TTGTTTTATCAAATGCTATT+TGG + chr5.2:73895165-73895184 MS.gene016820:CDS 20.0%
! ATCAAACAGACATTATTTAG+AGG - chr5.2:73894409-73894428 None:intergenic 25.0%
! TAAGATTGTTATCTAACAGT+TGG - chr5.2:73895078-73895097 None:intergenic 25.0%
!!! TTTCAATTTTTCGGTCATTT+TGG + chr5.2:73894997-73895016 MS.gene016820:CDS 25.0%
ACAAGAGAAAATACTTCTTG+AGG + chr5.2:73895215-73895234 MS.gene016820:CDS 30.0%
CATTAATGTAAACAAGACCT+CGG - chr5.2:73894561-73894580 None:intergenic 30.0%
CCTGCAAATCAAAAAAGAAA+AGG - chr5.2:73894870-73894889 None:intergenic 30.0%
GAAAGTAATTTATCCAAGCA+TGG + chr5.2:73894383-73894402 MS.gene016820:intron 30.0%
TCATCAAACAAAACATTTGC+AGG - chr5.2:73894619-73894638 None:intergenic 30.0%
TGTAAATGCATCAATGACAA+AGG - chr5.2:73894158-73894177 None:intergenic 30.0%
!! GAAGTATTTTCTCTTGTGTT+TGG - chr5.2:73895212-73895231 None:intergenic 30.0%
!! TTGCAGGCTTTAAATCATAA+TGG - chr5.2:73894603-73894622 None:intergenic 30.0%
!!! AATGTTTTGTTTGATGAGCT+TGG + chr5.2:73894622-73894641 MS.gene016820:CDS 30.0%
!!! CCTTTTCTTTTTTGATTTGC+AGG + chr5.2:73894867-73894886 MS.gene016820:intron 30.0%
ATAAACGAACAATGTGACGA+TGG - chr5.2:73894129-73894148 None:intergenic 35.0%
ATTTACATTCTGCACTGTTC+TGG + chr5.2:73894171-73894190 MS.gene016820:intron 35.0%
GGCAAAAGCTTAATGTTGAA+AGG - chr5.2:73894849-73894868 None:intergenic 35.0%
GTATGAGATTCAGAAGAACT+TGG + chr5.2:73894102-73894121 MS.gene016820:intron 35.0%
TCGTCACATTGTTCGTTTAT+TGG + chr5.2:73894129-73894148 MS.gene016820:intron 35.0%
TCTTGAGGACACGATTATTA+AGG + chr5.2:73895230-73895249 MS.gene016820:CDS 35.0%
TTTGCCTCATTAGAAACAGT+AGG - chr5.2:73895282-73895301 None:intergenic 35.0%
! ACATGTTCATAAGCCTGTTT+TGG + chr5.2:73894328-73894347 MS.gene016820:intron 35.0%
! ATTAGAAACAGTAGGTGTTG+AGG - chr5.2:73895274-73895293 None:intergenic 35.0%
! ATTTCTGACTTGTGGAAAAC+AGG + chr5.2:73895119-73895138 MS.gene016820:intron 35.0%
! CACTTTACATTTCTGACTTG+TGG + chr5.2:73895111-73895130 MS.gene016820:intron 35.0%
!! AACACCTACTGTTTCTAATG+AGG + chr5.2:73895275-73895294 MS.gene016820:CDS 35.0%
!! TTAGAAACAGTAGGTGTTGA+GGG - chr5.2:73895273-73895292 None:intergenic 35.0%
!! TTTGCGAAAGTGACTGATTT+TGG + chr5.2:73894646-73894665 MS.gene016820:CDS 35.0%
!! TTTTCGGTCATTTTGGTAAG+TGG + chr5.2:73895004-73895023 MS.gene016820:intron 35.0%
!!! TTTTGGTCTTAGCAAGATAG+TGG + chr5.2:73894663-73894682 MS.gene016820:CDS 35.0%
AAACAAGACCTCGGAAAATC+TGG - chr5.2:73894552-73894571 None:intergenic 40.0%
AAGCTTAAAACCGACAAGCT+TGG - chr5.2:73894794-73894813 None:intergenic 40.0%
CATTATTTAGAGGCCATGCT+TGG - chr5.2:73894399-73894418 None:intergenic 40.0%
CTCGGAAAATCTGGACTAAA+AGG - chr5.2:73894543-73894562 None:intergenic 40.0%
CTTATGGCCATAAACTCTCT+GGG + chr5.2:73894351-73894370 MS.gene016820:intron 40.0%
GTACATATTGGTAAGTGACG+AGG + chr5.2:73894734-73894753 MS.gene016820:intron 40.0%
TAGACCTTGGATGATATGAG+AGG - chr5.2:73894929-73894948 None:intergenic 40.0%
TCAAACTGTTCCAAGCTTGT+CGG + chr5.2:73894781-73894800 MS.gene016820:intron 40.0%
TTATGGCCATAAGCCAAAAC+AGG - chr5.2:73894344-73894363 None:intergenic 40.0%
! AAGATAGTGGCGAATGATGT+GGG + chr5.2:73894676-73894695 MS.gene016820:CDS 40.0%
! CTTTTAGTCCAGATTTTCCG+AGG + chr5.2:73894541-73894560 MS.gene016820:CDS 40.0%
!!! CATAAGCCTGTTTTGGCTTA+TGG + chr5.2:73894335-73894354 MS.gene016820:intron 40.0%
ACAGCATCAAGATCTTTCCC+TGG - chr5.2:73894478-73894497 None:intergenic 45.0%
CTATTTGGCAGACGTCCTTT+CGG + chr5.2:73895180-73895199 MS.gene016820:CDS 45.0%
CTTTCCCTGGTAGAGAACAA+GGG - chr5.2:73894465-73894484 None:intergenic 45.0%
GAGCAATCCCAGAGAGTTTA+TGG - chr5.2:73894361-73894380 None:intergenic 45.0%
GCTTATGGCCATAAACTCTC+TGG + chr5.2:73894350-73894369 MS.gene016820:intron 45.0%
GGATGTAAGTTCCATACCCT+GGG - chr5.2:73894704-73894723 None:intergenic 45.0%
GGTATGGAACTTACATCCCA+GGG + chr5.2:73894706-73894725 MS.gene016820:CDS 45.0%
TAGAGAACAAGGGGAGTCAA+AGG - chr5.2:73894455-73894474 None:intergenic 45.0%
TCTTTCCCTGGTAGAGAACA+AGG - chr5.2:73894466-73894485 None:intergenic 45.0%
TGGTAGACTGCGAGTATGTT+TGG + chr5.2:73894253-73894272 MS.gene016820:intron 45.0%
TGTGTTTGGTCATGTCCGAA+AGG - chr5.2:73895198-73895217 None:intergenic 45.0%
TTTCCCTGGTAGAGAACAAG+GGG - chr5.2:73894464-73894483 None:intergenic 45.0%
! CAAGATAGTGGCGAATGATG+TGG + chr5.2:73894675-73894694 MS.gene016820:CDS 45.0%
! TTGCTGCGTTCAAAGCATTC+AGG - chr5.2:73894902-73894921 None:intergenic 45.0%
!! ACTGTTCTGGAGTATTGCAG+TGG + chr5.2:73894184-73894203 MS.gene016820:intron 45.0%
!! GTGGAAAACAGGTTGATGTG+TGG + chr5.2:73895130-73895149 MS.gene016820:intron 45.0%
!!! ATAATTATTTTTACTTTTTT+TGG + chr5.2:73894233-73894252 MS.gene016820:intron 5.0%
ACCAATATGTACCAGCACCC+TGG - chr5.2:73894726-73894745 None:intergenic 50.0%
ACTCCCCTTGTTCTCTACCA+GGG + chr5.2:73894458-73894477 MS.gene016820:intron 50.0%
CCAATATGTACCAGCACCCT+GGG - chr5.2:73894725-73894744 None:intergenic 50.0%
GAACAAGGGGAGTCAAAGGA+AGG - chr5.2:73894451-73894470 None:intergenic 50.0%
GACGCCTCTCATATCATCCA+AGG + chr5.2:73894922-73894941 MS.gene016820:CDS 50.0%
GGGATGTAAGTTCCATACCC+TGG - chr5.2:73894705-73894724 None:intergenic 50.0%
GGGTATGGAACTTACATCCC+AGG + chr5.2:73894705-73894724 MS.gene016820:CDS 50.0%
GTGACGAGGATCGTTGTTGT+AGG + chr5.2:73894748-73894767 MS.gene016820:intron 50.0%
! CTGCGTTCAAAGCATTCAGG+AGG - chr5.2:73894899-73894918 None:intergenic 50.0%
!! CAGGTTGATGTGTGGTCTGT+CGG + chr5.2:73895138-73895157 MS.gene016820:intron 50.0%
AGGACTTTAGCCTCGCTCTC+TGG - chr5.2:73894523-73894542 None:intergenic 55.0%
GAACTTACATCCCAGGGTGC+TGG + chr5.2:73894712-73894731 MS.gene016820:CDS 55.0%
GACTCCCCTTGTTCTCTACC+AGG + chr5.2:73894457-73894476 MS.gene016820:intron 55.0%
GCCTATATTGCCAGAGAGCG+AGG + chr5.2:73894510-73894529 MS.gene016820:CDS 55.0%
GCCTCGCTCTCTGGCAATAT+AGG - chr5.2:73894514-73894533 None:intergenic 55.0%
GCGAATGATGTGGGATCCCA+GGG + chr5.2:73894685-73894704 MS.gene016820:CDS 55.0%
TGATGTGGGATCCCAGGGTA+TGG + chr5.2:73894690-73894709 MS.gene016820:CDS 55.0%
!! CCCAGGGTGCTGGTACATAT+TGG + chr5.2:73894722-73894741 MS.gene016820:CDS 55.0%
GGCGAATGATGTGGGATCCC+AGG + chr5.2:73894684-73894703 MS.gene016820:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 73894089 73895311 73894089 ID=MS.gene016820
chr5.2 mRNA 73894089 73895311 73894089 ID=MS.gene016820.t1;Parent=MS.gene016820
chr5.2 exon 73894089 73894101 73894089 ID=MS.gene016820.t1.exon1;Parent=MS.gene016820.t1
chr5.2 CDS 73894089 73894101 73894089 ID=cds.MS.gene016820.t1;Parent=MS.gene016820.t1
chr5.2 exon 73894479 73894743 73894479 ID=MS.gene016820.t1.exon2;Parent=MS.gene016820.t1
chr5.2 CDS 73894479 73894743 73894479 ID=cds.MS.gene016820.t1;Parent=MS.gene016820.t1
chr5.2 exon 73894889 73895018 73894889 ID=MS.gene016820.t1.exon3;Parent=MS.gene016820.t1
chr5.2 CDS 73894889 73895018 73894889 ID=cds.MS.gene016820.t1;Parent=MS.gene016820.t1
chr5.2 exon 73895141 73895311 73895141 ID=MS.gene016820.t1.exon4;Parent=MS.gene016820.t1
chr5.2 CDS 73895141 73895311 73895141 ID=cds.MS.gene016820.t1;Parent=MS.gene016820.t1
Gene Sequence

>MS.gene016820

ATGCAAAGCGGGAGTATGAGATTCAGAAGAACTTGGTCCATCGTCACATTGTTCGTTTATTGGACACCTTTGTCATTGATGCATTTACATTCTGCACTGTTCTGGAGTATTGCAGTGGTATATACACCTCATCTGTTTATGAAAATAATTATTTTTACTTTTTTTGGTAGACTGCGAGTATGTTTGGAATGAGTTATTTAAGCTTATATATTGGTAGAGAGAGCTTGTGAAAACTAATGACATGTTCATAAGCCTGTTTTGGCTTATGGCCATAAACTCTCTGGGATTGCTCATGAAAGTAATTTATCCAAGCATGGCCTCTAAATAATGTCTGTTTGATTTCTTGTTTCATCTATGATCCTTCCTTTGACTCCCCTTGTTCTCTACCAGGGAAAGATCTTGATGCTGTTCTTAAAGCAACGCCTATATTGCCAGAGAGCGAGGCTAAAGTCCTTTTAGTCCAGATTTTCCGAGGTCTTGTTTACATTAATGAAACAAAACCAAAGATTATCCATTATGATTTAAAGCCTGCAAATGTTTTGTTTGATGAGCTTGGTTTTGCGAAAGTGACTGATTTTGGTCTTAGCAAGATAGTGGCGAATGATGTGGGATCCCAGGGTATGGAACTTACATCCCAGGGTGCTGGTACATATTGGTAAGTGACGAGGATCGTTGTTGTAGGATTTCAAATTTCAAACTGTTCCAAGCTTGTCGGTTTTAAGCTTTAGTCTCTTTACTATTCTTTAACTTAGGTGCTCCTTTCAACATTAAGCTTTTGCCTTTTCTTTTTTGATTTGCAGGTATTTGCCTCCTGAATGCTTTGAACGCAGCAAGACGCCTCTCATATCATCCAAGGTCTATAATTTTTTAAGTTGTGAATACATAGATAGCTTTTTTTTGGTTACAATTTTCAATTTTTCGGTCATTTTGGTAAGTGGATATTTTGCTTTAATATAAGTTTCATGATGTCTGTATTCTGAAAACAACCAACTGTTAGATAACAATCTTACTTACATAATAATCACTTTACATTTCTGACTTGTGGAAAACAGGTTGATGTGTGGTCTGTCGGTATTTTGTTTTATCAAATGCTATTTGGCAGACGTCCTTTCGGACATGACCAAACACAAGAGAAAATACTTCTTGAGGACACGATTATTAAGGCACACAAAGTAGAATTTCCCTCAACACCTACTGTTTCTAATGAGGCAAAAGTGAGTTGA
Protein sequence

>MS.gene016820.t1

MQSGRKDLDAVLKATPILPESEAKVLLVQIFRGLVYINETKPKIIHYDLKPANVLFDELGFAKVTDFGLSKIVANDVGSQGMELTSQGAGTYWYLPPECFERSKTPLISSKVYNFLSCEYIDSFFLVTIFNFSVILVDVWSVGILFYQMLFGRRPFGHDQTQEKILLEDTIIKAHKVEFPSTPTVSNEAKVS