AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016881

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016881.t1 MTR_5g088060 85.841 452 53 2 1 449 1 444 0.0 751
MS.gene016881.t1 MTR_5g488160 85.841 452 53 2 1 449 1 444 0.0 751
MS.gene016881.t1 MTR_5g088010 82.759 232 32 3 226 449 1 232 8.86e-122 355
MS.gene016881.t1 MTR_5g088200 72.727 220 16 3 9 184 23 242 7.19e-94 303
MS.gene016881.t1 MTR_5g088005 82.822 163 15 1 5 167 12 161 6.76e-89 268
MS.gene016881.t1 MTR_5g027570 65.957 235 65 7 197 420 1 231 1.18e-85 262
MS.gene016881.t1 MTR_5g027550 47.479 238 91 6 197 416 1 222 2.11e-54 181
MS.gene016881.t1 MTR_5g027770 63.576 151 38 3 3 136 160 310 1.13e-47 166
MS.gene016881.t1 MTR_5g027770 41.538 130 28 2 7 133 31 115 3.07e-15 77.0
MS.gene016881.t1 MTR_5g027770 54.878 82 23 3 210 285 98 171 2.93e-14 73.9
MS.gene016881.t1 MTR_8g063600 45.000 120 63 3 238 355 62 180 8.93e-23 98.2
MS.gene016881.t1 MTR_5g027780 50.420 119 24 2 13 123 36 127 1.60e-22 95.1
MS.gene016881.t1 MTR_5g069710 58.974 78 28 2 282 358 134 208 4.88e-22 96.7
MS.gene016881.t1 MTR_8g063870 51.064 94 45 1 262 355 74 166 5.74e-22 95.5
MS.gene016881.t1 MTR_3g462790 64.286 70 21 2 282 350 149 215 6.31e-22 96.7
MS.gene016881.t1 MTR_1g111830 62.687 67 22 1 282 348 126 189 4.66e-21 92.4
MS.gene016881.t1 MTR_5g081860 56.790 81 33 2 269 348 113 192 5.22e-21 92.8
MS.gene016881.t1 MTR_7g067080 53.933 89 29 2 282 370 142 218 6.35e-21 93.6
MS.gene016881.t1 MTR_4g111975 41.880 117 58 5 278 392 135 243 5.26e-20 90.5
MS.gene016881.t1 MTR_1g048660 55.556 72 29 1 282 353 14 82 1.27e-18 80.9
MS.gene016881.t1 MTR_3g104370 48.571 70 36 0 282 351 93 162 2.78e-18 84.0
MS.gene016881.t1 MTR_2g100930 53.846 65 27 1 282 346 111 172 1.18e-17 84.3
MS.gene016881.t1 MTR_5g075760 40.517 116 66 2 237 352 64 176 3.83e-17 80.5
MS.gene016881.t1 MTR_4g100630 44.086 93 44 4 282 373 97 182 7.83e-17 81.6
MS.gene016881.t1 MTR_6g092540 52.308 65 28 1 282 346 99 160 5.82e-16 78.6
MS.gene016881.t1 MTR_8g101650 65.217 46 16 0 282 327 107 152 6.53e-16 78.2
MS.gene016881.t1 MTR_5g037080 67.391 46 15 0 282 327 104 149 7.14e-16 78.6
MS.gene016881.t1 MTR_4g015130 56.667 60 23 1 290 349 3 59 7.51e-16 72.8
MS.gene016881.t1 MTR_4g107230 68.889 45 14 0 282 326 86 130 9.41e-16 77.8
MS.gene016881.t1 MTR_2g090305 42.308 78 42 1 282 359 30 104 3.03e-15 74.3
MS.gene016881.t1 MTR_0036s0260 41.026 78 43 1 282 359 30 104 3.15e-15 74.7
MS.gene016881.t1 MTR_5g075790 38.889 108 56 2 254 352 78 184 3.35e-15 75.1
MS.gene016881.t1 MTR_1g033620 55.932 59 22 2 296 353 21 76 8.94e-14 71.2
MS.gene016881.t1 MTR_1g083180 38.095 126 57 7 248 355 13 135 9.71e-14 70.1
MS.gene016881.t1 MTR_3g070110 46.667 90 40 3 281 362 96 185 1.43e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016881.t1 AT5G58900 43.077 130 58 3 282 406 143 261 4.02e-23 99.4
MS.gene016881.t1 AT5G08520 56.790 81 31 2 278 357 115 192 1.33e-22 98.2
MS.gene016881.t1 AT5G04760 60.526 76 27 1 273 348 92 164 1.70e-22 95.9
MS.gene016881.t1 AT5G01200 61.429 70 23 2 282 350 148 214 3.39e-21 93.2
MS.gene016881.t1 AT2G38090 57.692 78 28 3 275 350 135 209 2.07e-20 91.7
MS.gene016881.t1 AT1G49010 53.659 82 33 3 278 357 131 209 3.43e-20 91.3
MS.gene016881.t1 AT5G61620 54.545 77 31 2 274 349 101 174 4.80e-19 88.2
MS.gene016881.t1 AT3G11280 43.443 122 50 5 282 392 129 242 7.47e-19 86.7
MS.gene016881.t1 AT3G11280 43.443 122 50 5 282 392 129 242 7.47e-19 86.7
MS.gene016881.t1 AT5G23650 52.632 76 33 1 282 357 122 194 4.06e-18 85.5
MS.gene016881.t1 AT5G05790 55.000 80 28 3 278 352 128 204 1.56e-17 82.8
MS.gene016881.t1 AT5G05790 55.000 80 28 3 278 352 128 204 1.56e-17 82.8
MS.gene016881.t1 AT3G10580 44.444 99 42 3 269 360 77 169 1.68e-17 82.4
MS.gene016881.t1 AT3G10580 44.444 99 42 3 269 360 77 169 2.04e-17 82.8
MS.gene016881.t1 AT5G47390 46.591 88 41 3 282 367 98 181 2.89e-17 83.6
MS.gene016881.t1 AT1G70000 67.391 46 15 0 282 327 100 145 2.04e-16 79.3
MS.gene016881.t1 AT1G70000 67.391 46 15 0 282 327 100 145 2.04e-16 79.3
MS.gene016881.t1 AT5G56840 55.172 58 26 0 282 339 93 150 2.53e-16 78.6
MS.gene016881.t1 AT4G09450 54.412 68 28 1 282 349 93 157 3.65e-16 77.4
MS.gene016881.t1 AT3G16350 65.217 46 16 0 282 327 139 184 1.17e-15 79.0
MS.gene016881.t1 AT1G74840 68.889 45 14 0 282 326 98 142 1.82e-15 76.3
MS.gene016881.t1 AT1G74840 68.889 45 14 0 282 326 98 142 2.56e-15 76.3
MS.gene016881.t1 AT1G19000 68.889 45 14 0 282 326 104 148 2.80e-15 76.6
MS.gene016881.t1 AT1G19000 68.889 45 14 0 282 326 104 148 2.80e-15 76.6
MS.gene016881.t1 AT3G10590 50.000 68 30 2 282 349 113 176 1.66e-12 67.0

Find 108 sgRNAs with CRISPR-Local

Find 166 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATATCTTTCACATTT+TGG 0.099316 5.2:-74614985 None:intergenic
TCACCTTGTTCTTGGATATT+TGG 0.151843 5.2:-74613658 None:intergenic
TCAACATGATTCATAGGTTT+TGG 0.219569 5.2:-74612928 None:intergenic
CCTGCTGCTGCCGGGGATAC+AGG 0.250754 5.2:-74613532 None:intergenic
CTGTCCAATGCCCTTTCCTT+GGG 0.257949 5.2:-74614612 None:intergenic
CTTGGGGCATGACTTTCTTC+TGG 0.281577 5.2:-74614595 None:intergenic
TCCTAGAGCTCCTTCTGTTC+AGG 0.288148 5.2:+74612984 MS.gene016881:CDS
TCACATGGTCACCTTGTTCT+TGG 0.300281 5.2:-74613666 None:intergenic
TCTGTCCAATGCCCTTTCCT+TGG 0.314383 5.2:-74614613 None:intergenic
TGGATATTTGGCCTTCCATT+TGG 0.317326 5.2:-74613646 None:intergenic
CCACCAAAATGTGAAAGATA+TGG 0.352224 5.2:+74614982 MS.gene016881:CDS
CACCGGAGGCTGAACATGTT+TGG 0.362724 5.2:-74613590 None:intergenic
ACATTCTCAACATGATTCAT+AGG 0.366509 5.2:-74612934 None:intergenic
GATGTTCCTGTGTTGGTTGT+TGG 0.372353 5.2:+74612868 MS.gene016881:CDS
AAACAGAAATCAAACGGTTC+TGG 0.380690 5.2:+74613376 MS.gene016881:intron
TTTCTGTTGCGCTTGGGGCC+TGG 0.382290 5.2:-74613445 None:intergenic
TGCTATGCTCAGAGGGATCT+TGG 0.382368 5.2:-74614438 None:intergenic
AACCTTGTCCTGCTGCTGCC+GGG 0.402092 5.2:-74613540 None:intergenic
TTCTGTGCCTAGTGTTGGTC+AGG 0.402578 5.2:+74612957 MS.gene016881:CDS
CCTCCTCAAGACACTGTGTT+AGG 0.410416 5.2:+74615280 MS.gene016881:CDS
GGTGATGGCATCTTCTTCTT+CGG 0.411483 5.2:-74613409 None:intergenic
TTTGTGTTTCTGTTGCGCTT+GGG 0.412088 5.2:-74613451 None:intergenic
CTGAACATGTTTGGCATCAA+TGG 0.415429 5.2:-74613581 None:intergenic
AAATATTTCAAAAGAATTTG+TGG 0.425853 5.2:+74614910 MS.gene016881:CDS
ATTTGTGTTTCTGTTGCGCT+TGG 0.430827 5.2:-74613452 None:intergenic
TCCTTTAGTCACTGCTCCTA+TGG 0.430956 5.2:+74615240 MS.gene016881:CDS
GGATTCTCGCCATTAGGATA+AGG 0.435944 5.2:-74613505 None:intergenic
TATGCTGTTAATTATCCAAA+TGG 0.436533 5.2:+74613631 MS.gene016881:CDS
GGCTTAGGATTCTCGCCATT+AGG 0.439906 5.2:-74613511 None:intergenic
TGTGAAAGATATGGAGAAGA+AGG 0.441203 5.2:+74614991 MS.gene016881:CDS
GAACAACAACAGGAGAAAAC+TGG 0.441684 5.2:+74614463 MS.gene016881:CDS
AATCGGTTCTACAATTCCTT+CGG 0.444800 5.2:+74615195 MS.gene016881:CDS
AAACCTTGTCCTGCTGCTGC+CGG 0.447814 5.2:-74613541 None:intergenic
ACCACCCGACTCAATGATAT+CGG 0.449690 5.2:-74614488 None:intergenic
ATGTTTGCCCTGGCAGGAAA+AGG 0.462283 5.2:-74613712 None:intergenic
GCGACGATGCCTTATCCTAA+TGG 0.462440 5.2:+74613496 MS.gene016881:CDS
CCTGTATCCCCGGCAGCAGC+AGG 0.464792 5.2:+74613532 MS.gene016881:CDS
GGCTTTCGATAGCGCGGTGA+TGG 0.464836 5.2:-74613424 None:intergenic
GCTCATTTAAATCAATTTGA+AGG 0.475975 5.2:-74614405 None:intergenic
TCAAATTGATTTAAATGAGC+CGG 0.477484 5.2:+74614408 MS.gene016881:CDS
TTGTGTTTCTGTTGCGCTTG+GGG 0.482706 5.2:-74613450 None:intergenic
GCATAGCATGGAACAACAAC+AGG 0.485938 5.2:+74614453 MS.gene016881:CDS
TCACCGCGCTATCGAAAGCC+AGG 0.491564 5.2:+74613427 MS.gene016881:CDS
AGAAAGAGCTGGCGAAGACT+TGG 0.491824 5.2:-74612893 None:intergenic
GCTGCCGGGGATACAGGCTT+AGG 0.492601 5.2:-74613526 None:intergenic
AAAGTCATGCCCCAAGGAAA+GGG 0.493395 5.2:+74614602 MS.gene016881:CDS
ATGTTGTGCTTGCTGCCAAT+CGG 0.496477 5.2:+74615178 MS.gene016881:CDS
GAATCCTAAGCCTGTATCCC+CGG 0.504373 5.2:+74613522 MS.gene016881:CDS
ATAGGCATGTTTGCCCTGGC+AGG 0.507217 5.2:-74613718 None:intergenic
TGCCAAACATGTTCAGCCTC+CGG 0.511829 5.2:+74613588 MS.gene016881:CDS
TAATTAACAGCATAAGCATT+CGG 0.516489 5.2:-74613622 None:intergenic
TACTGTGTTGCATAGGGCAT+AGG 0.522213 5.2:-74613736 None:intergenic
ATGCCCCAAGGAAAGGGCAT+TGG 0.524782 5.2:+74614608 MS.gene016881:CDS
GCATGGCTAGCAATTTGAGT+TGG 0.525594 5.2:-74614944 None:intergenic
GAAAGTCATGCCCCAAGGAA+AGG 0.527463 5.2:+74614601 MS.gene016881:CDS
GTGATTAAACAGAAATCAAA+CGG 0.527832 5.2:+74613370 MS.gene016881:intron
GTAATTATGGTGAGGATCAG+AGG 0.529504 5.2:+74612808 None:intergenic
GTCTCATGTTGTTGTTCCAT+AGG 0.529730 5.2:-74615256 None:intergenic
GGCACCGATATCATTGAGTC+GGG 0.531762 5.2:+74614484 MS.gene016881:CDS
CAAGGTGACCATGTGAATCA+TGG 0.533701 5.2:+74613673 MS.gene016881:CDS
TGTTCCATGCTATGCTCAGA+GGG 0.536332 5.2:-74614445 None:intergenic
ATTAGAGTTTAGAATTGCAA+TGG 0.542743 5.2:-74614512 None:intergenic
AATGTTTCTGTGCCTAGTGT+TGG 0.550067 5.2:+74612952 MS.gene016881:CDS
GGATAAGGCATCGTCGCCAC+AGG 0.553867 5.2:-74613490 None:intergenic
ATTTAAATCAATTTGAAGGA+GGG 0.554059 5.2:-74614401 None:intergenic
TCCATAGGAGCAGTGACTAA+AGG 0.557140 5.2:-74615241 None:intergenic
GTCTGTTGCATTCCTTGAGA+AGG 0.557189 5.2:-74615127 None:intergenic
TTCGGATCTCCTTGCACCGG+AGG 0.558249 5.2:-74613604 None:intergenic
TGTCCAATGCCCTTTCCTTG+GGG 0.558952 5.2:-74614611 None:intergenic
GGGCATAGGCATGTTTGCCC+TGG 0.560819 5.2:-74613722 None:intergenic
ACCTGAACAGAAGGAGCTCT+AGG 0.562137 5.2:-74612985 None:intergenic
CATGTTCAGCCTCCGGTGCA+AGG 0.562364 5.2:+74613595 MS.gene016881:CDS
AACAATCGAACCTGAACAGA+AGG 0.565912 5.2:-74612994 None:intergenic
ACCTTGTCCTGCTGCTGCCG+GGG 0.566255 5.2:-74613539 None:intergenic
TTGTTCCATGCTATGCTCAG+AGG 0.567759 5.2:-74614446 None:intergenic
GAGGGATCTTGGACTGAAGC+CGG 0.570243 5.2:-74614427 None:intergenic
CATTTAAATCAATTTGAAGG+AGG 0.570848 5.2:-74614402 None:intergenic
TCCCCGGCAGCAGCAGGACA+AGG 0.572616 5.2:+74613538 MS.gene016881:CDS
AAGTGTTGATGTTCCTGTGT+TGG 0.580284 5.2:+74612861 MS.gene016881:CDS
TGGCACCGATATCATTGAGT+CGG 0.583638 5.2:+74614483 MS.gene016881:CDS
CAAAGTGTGATTCCTTCTCA+AGG 0.586591 5.2:+74615115 MS.gene016881:CDS
GAAGAAAAGTAATTATGGTG+AGG 0.586984 5.2:+74612800 None:intergenic
ATTGGCAGCAAGCACAACAT+AGG 0.587278 5.2:-74615175 None:intergenic
GTTTGAGCATGAGAAAGAGC+TGG 0.587648 5.2:-74612904 None:intergenic
ACACTTTGTAGCTCAACTGA+AGG 0.589388 5.2:-74615100 None:intergenic
GGAGCAACCTGACCAACACT+AGG 0.591510 5.2:-74612964 None:intergenic
AGATCCCTCTGAGCATAGCA+TGG 0.596307 5.2:+74614441 MS.gene016881:CDS
CTGTTAATTATCCAAATGGA+AGG 0.601168 5.2:+74613635 MS.gene016881:CDS
GAAGGAATTGTAGAACCGAT+TGG 0.605792 5.2:-74615193 None:intergenic
ATTAAACAAGAATGACACAT+TGG 0.608999 5.2:+74615045 MS.gene016881:CDS
GCATCAATGGCATGAGCAGG+TGG 0.609092 5.2:-74613568 None:intergenic
ACCGATATCATTGAGTCGGG+TGG 0.615037 5.2:+74614487 MS.gene016881:CDS
GCATTCGGATCTCCTTGCAC+CGG 0.618195 5.2:-74613607 None:intergenic
ATCGTCGCCACAGGAAAGTG+AGG 0.619216 5.2:-74613481 None:intergenic
TTGGCATCAATGGCATGAGC+AGG 0.623729 5.2:-74613571 None:intergenic
TCACCTAACACAGTGTCTTG+AGG 0.631142 5.2:-74615283 None:intergenic
CAGAAGAAAGTCATGCCCCA+AGG 0.636413 5.2:+74614596 MS.gene016881:CDS
GTGACCATGTGAATCATGGT+CGG 0.644274 5.2:+74613677 MS.gene016881:CDS
AGGCCAAATATCCAAGAACA+AGG 0.648452 5.2:+74613655 MS.gene016881:CDS
GGGCCTGGCTTTCGATAGCG+CGG 0.653594 5.2:-74613430 None:intergenic
ATTATGGTGAGGATCAGAGG+AGG 0.653905 5.2:+74612811 None:intergenic
AAGGAAAGGGCATTGGACAG+AGG 0.658250 5.2:+74614615 MS.gene016881:CDS
GGATCAGAGGAGGAAAGCAG+AGG 0.659593 5.2:+74612821 MS.gene016881:CDS
TTGGTTCCAACAACCAACAC+AGG 0.665985 5.2:-74612874 None:intergenic
CGTCACAACTCTAAACTCTG+AGG 0.678462 5.2:+74614543 MS.gene016881:CDS
CCTAACACAGTGTCTTGAGG+AGG 0.684594 5.2:-74615280 None:intergenic
TGACACGCCTCACTTTCCTG+TGG 0.696402 5.2:+74613474 MS.gene016881:CDS
GATGCCGACCATGATTCACA+TGG 0.766610 5.2:-74613681 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAACTTTCAAACAT+AGG - chr5.2:74614679-74614698 None:intergenic 15.0%
!! AAATATTTCAAAAGAATTTG+TGG + chr5.2:74614910-74614929 MS.gene016881:CDS 15.0%
!!! AATTCTTATAGTTGTATTTT+TGG + chr5.2:74614340-74614359 MS.gene016881:intron 15.0%
!!! ATTTTGTTAACTTACATAAT+TGG + chr5.2:74614144-74614163 MS.gene016881:intron 15.0%
!!! CAAAAAACAGAATAATTTTA+TGG - chr5.2:74614758-74614777 None:intergenic 15.0%
!! ATTTAAATCAATTTGAAGGA+GGG - chr5.2:74614404-74614423 None:intergenic 20.0%
!! TAATTCAAATTGGATGAAAT+TGG + chr5.2:74614312-74614331 MS.gene016881:intron 20.0%
!! TGTAAGTTAACAAAATTACT+TGG - chr5.2:74614140-74614159 None:intergenic 20.0%
!!! ATATATAGGTTGTTTTTGAA+GGG + chr5.2:74614857-74614876 MS.gene016881:intron 20.0%
!!! CATGCATAATATTTTTTGAA+TGG - chr5.2:74614286-74614305 None:intergenic 20.0%
!!! TATATATAGGTTGTTTTTGA+AGG + chr5.2:74614856-74614875 MS.gene016881:intron 20.0%
! AAGAATTAAGATGATTTAGC+TGG + chr5.2:74614243-74614262 MS.gene016881:intron 25.0%
! ATTAAACAAGAATGACACAT+TGG + chr5.2:74615045-74615064 MS.gene016881:CDS 25.0%
! ATTATGATATCTTCTCGTTA+TGG + chr5.2:74614089-74614108 MS.gene016881:intron 25.0%
! CACATTCATTCTCTATATAT+AGG + chr5.2:74614843-74614862 MS.gene016881:intron 25.0%
! CATGTCGATATAATTCAAAT+TGG + chr5.2:74614302-74614321 MS.gene016881:intron 25.0%
! CATTTAAATCAATTTGAAGG+AGG - chr5.2:74614405-74614424 None:intergenic 25.0%
! GCTCATTTAAATCAATTTGA+AGG - chr5.2:74614408-74614427 None:intergenic 25.0%
! GTGATTAAACAGAAATCAAA+CGG + chr5.2:74613370-74613389 MS.gene016881:intron 25.0%
! TAAACAACGAGTGAAAAATA+TGG + chr5.2:74613215-74613234 MS.gene016881:intron 25.0%
! TAATTAACAGCATAAGCATT+CGG - chr5.2:74613625-74613644 None:intergenic 25.0%
! TATGCTGTTAATTATCCAAA+TGG + chr5.2:74613631-74613650 MS.gene016881:CDS 25.0%
! TGTTAAGCTAAATGAATAAG+TGG - chr5.2:74613808-74613827 None:intergenic 25.0%
! TTCTTATGCTTGCAATAATA+TGG - chr5.2:74613152-74613171 None:intergenic 25.0%
!! AAATTTTACTGTGTTGCATA+GGG - chr5.2:74613745-74613764 None:intergenic 25.0%
!! ATTAGAGTTTAGAATTGCAA+TGG - chr5.2:74614515-74614534 None:intergenic 25.0%
!! TCAAATTGATTTAAATGAGC+CGG + chr5.2:74614408-74614427 MS.gene016881:CDS 25.0%
!! TCTAGAATATTCTTAACTCT+GGG + chr5.2:74613302-74613321 MS.gene016881:intron 25.0%
!! TTAGTGTAATGTGATTTTGA+TGG + chr5.2:74613071-74613090 MS.gene016881:intron 25.0%
!!! TATATAGGTTGTTTTTGAAG+GGG + chr5.2:74614858-74614877 MS.gene016881:intron 25.0%
!!! TATTTTTGGTATAGAAAGCA+TGG + chr5.2:74614354-74614373 MS.gene016881:CDS 25.0%
!!! TTCTAGAATATTCTTAACTC+TGG + chr5.2:74613301-74613320 MS.gene016881:intron 25.0%
!!! TTGGTGGATAAAGTATTTTT+GGG - chr5.2:74614969-74614988 None:intergenic 25.0%
!!! TTTGGTGGATAAAGTATTTT+TGG - chr5.2:74614970-74614989 None:intergenic 25.0%
AATTGCATGAAGTGCATATA+TGG - chr5.2:74613783-74613802 None:intergenic 30.0%
ACATTCTCAACATGATTCAT+AGG - chr5.2:74612937-74612956 None:intergenic 30.0%
ACTCAATGAAGTTCATTCAA+AGG - chr5.2:74613848-74613867 None:intergenic 30.0%
AGAAGGAGAAAAAGAGAAAA+AGG + chr5.2:74615008-74615027 MS.gene016881:CDS 30.0%
CTGTTAATTATCCAAATGGA+AGG + chr5.2:74613635-74613654 MS.gene016881:CDS 30.0%
TTACTTTGATACCATTGAAG+TGG + chr5.2:74614020-74614039 MS.gene016881:intron 30.0%
TTTAATCACAGATCCATACA+CGG - chr5.2:74613359-74613378 None:intergenic 30.0%
! GAAATTTTACTGTGTTGCAT+AGG - chr5.2:74613746-74613765 None:intergenic 30.0%
! TCAACATGATTCATAGGTTT+TGG - chr5.2:74612931-74612950 None:intergenic 30.0%
! TCTCCATATCTTTCACATTT+TGG - chr5.2:74614988-74615007 None:intergenic 30.0%
!!! ATTGTTTTTTTTCCCTTCGA+TGG + chr5.2:74613264-74613283 MS.gene016881:intron 30.0%
AAACAGAAATCAAACGGTTC+TGG + chr5.2:74613376-74613395 MS.gene016881:intron 35.0%
CCACCAAAATGTGAAAGATA+TGG + chr5.2:74614982-74615001 MS.gene016881:CDS 35.0%
GGGATGAAAAAACATGGAAA+GGG + chr5.2:74614878-74614897 MS.gene016881:CDS 35.0%
TCACCTTGTTCTTGGATATT+TGG - chr5.2:74613661-74613680 None:intergenic 35.0%
TGTGAAAGATATGGAGAAGA+AGG + chr5.2:74614991-74615010 MS.gene016881:CDS 35.0%
TTGAAGGGGATGAAAAAACA+TGG + chr5.2:74614872-74614891 MS.gene016881:CDS 35.0%
! AATCGGTTCTACAATTCCTT+CGG + chr5.2:74615195-74615214 MS.gene016881:CDS 35.0%
! CCATATCTTTCACATTTTGG+TGG - chr5.2:74614985-74615004 None:intergenic 35.0%
! TCTCGTTATGGTTCTGTAAT+TGG + chr5.2:74614101-74614120 MS.gene016881:intron 35.0%
! TTGGCTAGTTCATCTGATTA+AGG + chr5.2:74614163-74614182 MS.gene016881:intron 35.0%
!! ACAGCTTTTATTTCCATCGA+AGG - chr5.2:74613280-74613299 None:intergenic 35.0%
!! CAGCTTTTATTTCCATCGAA+GGG - chr5.2:74613279-74613298 None:intergenic 35.0%
!! CTGTAATTGGTTTGAGTAAC+TGG + chr5.2:74614114-74614133 MS.gene016881:intron 35.0%
!!! CATCGTTTTTTGAGTAAACC+AGG + chr5.2:74614206-74614225 MS.gene016881:intron 35.0%
!!! GGATAAAGTATTTTTGGGCA+TGG - chr5.2:74614964-74614983 None:intergenic 35.0%
!!! TTTTTTGAGTAAACCAGGTG+AGG + chr5.2:74614211-74614230 MS.gene016881:intron 35.0%
AACAATCGAACCTGAACAGA+AGG - chr5.2:74612997-74613016 None:intergenic 40.0%
ACACTTTGTAGCTCAACTGA+AGG - chr5.2:74615103-74615122 None:intergenic 40.0%
AGATCCATACACGGAAAATC+AGG - chr5.2:74613350-74613369 None:intergenic 40.0%
AGGCCAAATATCCAAGAACA+AGG + chr5.2:74613655-74613674 MS.gene016881:CDS 40.0%
CAAAGTGTGATTCCTTCTCA+AGG + chr5.2:74615115-74615134 MS.gene016881:CDS 40.0%
CTGAACATGTTTGGCATCAA+TGG - chr5.2:74613584-74613603 None:intergenic 40.0%
GAACAACAACAGGAGAAAAC+TGG + chr5.2:74614463-74614482 MS.gene016881:CDS 40.0%
GAAGGAATTGTAGAACCGAT+TGG - chr5.2:74615196-74615215 None:intergenic 40.0%
GCAACTAAAAGCCACTTCAA+TGG - chr5.2:74614034-74614053 None:intergenic 40.0%
GGGGATGAAAAAACATGGAA+AGG + chr5.2:74614877-74614896 MS.gene016881:CDS 40.0%
TGCATGAAGTGCATATATGG+AGG - chr5.2:74613780-74613799 None:intergenic 40.0%
! AATGTTTCTGTGCCTAGTGT+TGG + chr5.2:74612952-74612971 MS.gene016881:CDS 40.0%
! ATTTGTGTTTCTGTTGCGCT+TGG - chr5.2:74613455-74613474 None:intergenic 40.0%
! GTCTCATGTTGTTGTTCCAT+AGG - chr5.2:74615259-74615278 None:intergenic 40.0%
! TGGATATTTGGCCTTCCATT+TGG - chr5.2:74613649-74613668 None:intergenic 40.0%
! TTTGCCTGATTTTCCGTGTA+TGG + chr5.2:74613343-74613362 MS.gene016881:intron 40.0%
! TTTGTGTTTCTGTTGCGCTT+GGG - chr5.2:74613454-74613473 None:intergenic 40.0%
!! AAGATTCTAGCTTAACCCTC+TGG + chr5.2:74613938-74613957 MS.gene016881:intron 40.0%
!! AAGTGTTGATGTTCCTGTGT+TGG + chr5.2:74612861-74612880 MS.gene016881:CDS 40.0%
!! AATACCCAAAAAAGCACCAG+AGG - chr5.2:74613957-74613976 None:intergenic 40.0%
!! ATACCCAAAAAAGCACCAGA+GGG - chr5.2:74613956-74613975 None:intergenic 40.0%
!!! TAACCCTCTGGTGCTTTTTT+GGG + chr5.2:74613950-74613969 MS.gene016881:intron 40.0%
!!! TTAACCCTCTGGTGCTTTTT+TGG + chr5.2:74613949-74613968 MS.gene016881:intron 40.0%
AAAAACATGGAAAGGGACGC+TGG + chr5.2:74614885-74614904 MS.gene016881:CDS 45.0%
AAAGTCATGCCCCAAGGAAA+GGG + chr5.2:74614602-74614621 MS.gene016881:CDS 45.0%
ACCACCCGACTCAATGATAT+CGG - chr5.2:74614491-74614510 None:intergenic 45.0%
ATTGGCAGCAAGCACAACAT+AGG - chr5.2:74615178-74615197 None:intergenic 45.0%
CAAGGTGACCATGTGAATCA+TGG + chr5.2:74613673-74613692 MS.gene016881:CDS 45.0%
CGTCACAACTCTAAACTCTG+AGG + chr5.2:74614543-74614562 MS.gene016881:CDS 45.0%
GCATAGCATGGAACAACAAC+AGG + chr5.2:74614453-74614472 MS.gene016881:CDS 45.0%
GCATGGCTAGCAATTTGAGT+TGG - chr5.2:74614947-74614966 None:intergenic 45.0%
GCGAGTCGACAAATCTGTTT+AGG + chr5.2:74613237-74613256 MS.gene016881:intron 45.0%
GGATTCTCGCCATTAGGATA+AGG - chr5.2:74613508-74613527 None:intergenic 45.0%
GTCTGTTGCATTCCTTGAGA+AGG - chr5.2:74615130-74615149 None:intergenic 45.0%
GTGACCATGTGAATCATGGT+CGG + chr5.2:74613677-74613696 MS.gene016881:CDS 45.0%
GTTTGAGCATGAGAAAGAGC+TGG - chr5.2:74612907-74612926 None:intergenic 45.0%
TACTGTGTTGCATAGGGCAT+AGG - chr5.2:74613739-74613758 None:intergenic 45.0%
TCACATGGTCACCTTGTTCT+TGG - chr5.2:74613669-74613688 None:intergenic 45.0%
TCACCTAACACAGTGTCTTG+AGG - chr5.2:74615286-74615305 None:intergenic 45.0%
TCCATAGGAGCAGTGACTAA+AGG - chr5.2:74615244-74615263 None:intergenic 45.0%
TCCTTTAGTCACTGCTCCTA+TGG + chr5.2:74615240-74615259 MS.gene016881:CDS 45.0%
TGTTCCATGCTATGCTCAGA+GGG - chr5.2:74614448-74614467 None:intergenic 45.0%
TTGGTTCCAACAACCAACAC+AGG - chr5.2:74612877-74612896 None:intergenic 45.0%
TTGTTCCATGCTATGCTCAG+AGG - chr5.2:74614449-74614468 None:intergenic 45.0%
! ATCAAAGACCTTTTCCTGCC+AGG + chr5.2:74613704-74613723 MS.gene016881:CDS 45.0%
! ATGTTGTGCTTGCTGCCAAT+CGG + chr5.2:74615178-74615197 MS.gene016881:CDS 45.0%
! GGTGATGGCATCTTCTTCTT+CGG - chr5.2:74613412-74613431 None:intergenic 45.0%
! TCAAAGACCTTTTCCTGCCA+GGG + chr5.2:74613705-74613724 MS.gene016881:CDS 45.0%
! TTGTGTTTCTGTTGCGCTTG+GGG - chr5.2:74613453-74613472 None:intergenic 45.0%
!! GATGTTCCTGTGTTGGTTGT+TGG + chr5.2:74612868-74612887 MS.gene016881:CDS 45.0%
!! TCGTTTTGTTGCTCAACCGA+AGG - chr5.2:74615214-74615233 None:intergenic 45.0%
!! TGGCACCGATATCATTGAGT+CGG + chr5.2:74614483-74614502 MS.gene016881:CDS 45.0%
ACCGATATCATTGAGTCGGG+TGG + chr5.2:74614487-74614506 MS.gene016881:CDS 50.0%
ACCTGAACAGAAGGAGCTCT+AGG - chr5.2:74612988-74613007 None:intergenic 50.0%
AGAAAGAGCTGGCGAAGACT+TGG - chr5.2:74612896-74612915 None:intergenic 50.0%
AGATCCCTCTGAGCATAGCA+TGG + chr5.2:74614441-74614460 MS.gene016881:CDS 50.0%
ATGTTTGCCCTGGCAGGAAA+AGG - chr5.2:74613715-74613734 None:intergenic 50.0%
CAGAAGAAAGTCATGCCCCA+AGG + chr5.2:74614596-74614615 MS.gene016881:CDS 50.0%
CCTAACACAGTGTCTTGAGG+AGG - chr5.2:74615283-74615302 None:intergenic 50.0%
CCTCCTCAAGACACTGTGTT+AGG + chr5.2:74615280-74615299 MS.gene016881:CDS 50.0%
CTGTCCAATGCCCTTTCCTT+GGG - chr5.2:74614615-74614634 None:intergenic 50.0%
CTTGGGGCATGACTTTCTTC+TGG - chr5.2:74614598-74614617 None:intergenic 50.0%
GAAAGTCATGCCCCAAGGAA+AGG + chr5.2:74614601-74614620 MS.gene016881:CDS 50.0%
GAATCCTAAGCCTGTATCCC+CGG + chr5.2:74613522-74613541 MS.gene016881:CDS 50.0%
GATGCCGACCATGATTCACA+TGG - chr5.2:74613684-74613703 None:intergenic 50.0%
GCGACGATGCCTTATCCTAA+TGG + chr5.2:74613496-74613515 MS.gene016881:CDS 50.0%
GGCTTAGGATTCTCGCCATT+AGG - chr5.2:74613514-74613533 None:intergenic 50.0%
TCCTAGAGCTCCTTCTGTTC+AGG + chr5.2:74612984-74613003 MS.gene016881:CDS 50.0%
TCTGTCCAATGCCCTTTCCT+TGG - chr5.2:74614616-74614635 None:intergenic 50.0%
TGAGTAAACCAGGTGAGGCT+GGG + chr5.2:74614216-74614235 MS.gene016881:intron 50.0%
TGCCAAACATGTTCAGCCTC+CGG + chr5.2:74613588-74613607 MS.gene016881:CDS 50.0%
TGCTATGCTCAGAGGGATCT+TGG - chr5.2:74614441-74614460 None:intergenic 50.0%
TGTCCAATGCCCTTTCCTTG+GGG - chr5.2:74614614-74614633 None:intergenic 50.0%
TTGAGTAAACCAGGTGAGGC+TGG + chr5.2:74614215-74614234 MS.gene016881:intron 50.0%
TTGGCATCAATGGCATGAGC+AGG - chr5.2:74613574-74613593 None:intergenic 50.0%
!! AAGGAAAGGGCATTGGACAG+AGG + chr5.2:74614615-74614634 MS.gene016881:CDS 50.0%
!! GGCACCGATATCATTGAGTC+GGG + chr5.2:74614484-74614503 MS.gene016881:CDS 50.0%
!! TTCTGTGCCTAGTGTTGGTC+AGG + chr5.2:74612957-74612976 MS.gene016881:CDS 50.0%
AAACCTTGTCCTGCTGCTGC+CGG - chr5.2:74613544-74613563 None:intergenic 55.0%
ATAGGCATGTTTGCCCTGGC+AGG - chr5.2:74613721-74613740 None:intergenic 55.0%
ATCGTCGCCACAGGAAAGTG+AGG - chr5.2:74613484-74613503 None:intergenic 55.0%
CACCGGAGGCTGAACATGTT+TGG - chr5.2:74613593-74613612 None:intergenic 55.0%
GAGGGATCTTGGACTGAAGC+CGG - chr5.2:74614430-74614449 None:intergenic 55.0%
GAGTAAACCAGGTGAGGCTG+GGG + chr5.2:74614217-74614236 MS.gene016881:intron 55.0%
GCATCAATGGCATGAGCAGG+TGG - chr5.2:74613571-74613590 None:intergenic 55.0%
GCATTCGGATCTCCTTGCAC+CGG - chr5.2:74613610-74613629 None:intergenic 55.0%
GGAGCAACCTGACCAACACT+AGG - chr5.2:74612967-74612986 None:intergenic 55.0%
GGATCAGAGGAGGAAAGCAG+AGG + chr5.2:74612821-74612840 MS.gene016881:CDS 55.0%
TGACACGCCTCACTTTCCTG+TGG + chr5.2:74613474-74613493 MS.gene016881:CDS 55.0%
! ATGCCCCAAGGAAAGGGCAT+TGG + chr5.2:74614608-74614627 MS.gene016881:CDS 55.0%
AACCTTGTCCTGCTGCTGCC+GGG - chr5.2:74613543-74613562 None:intergenic 60.0%
CATGTTCAGCCTCCGGTGCA+AGG + chr5.2:74613595-74613614 MS.gene016881:CDS 60.0%
GGCTTTCGATAGCGCGGTGA+TGG - chr5.2:74613427-74613446 None:intergenic 60.0%
GGGCATAGGCATGTTTGCCC+TGG - chr5.2:74613725-74613744 None:intergenic 60.0%
TCACCGCGCTATCGAAAGCC+AGG + chr5.2:74613427-74613446 MS.gene016881:CDS 60.0%
TCTTAGTCCCCAGCCTCACC+TGG - chr5.2:74614227-74614246 None:intergenic 60.0%
TTCGGATCTCCTTGCACCGG+AGG - chr5.2:74613607-74613626 None:intergenic 60.0%
TTTCTGTTGCGCTTGGGGCC+TGG - chr5.2:74613448-74613467 None:intergenic 60.0%
!! GGATAAGGCATCGTCGCCAC+AGG - chr5.2:74613493-74613512 None:intergenic 60.0%
ACCTTGTCCTGCTGCTGCCG+GGG - chr5.2:74613542-74613561 None:intergenic 65.0%
GCTGCCGGGGATACAGGCTT+AGG - chr5.2:74613529-74613548 None:intergenic 65.0%
! GGGCCTGGCTTTCGATAGCG+CGG - chr5.2:74613433-74613452 None:intergenic 65.0%
CCTGCTGCTGCCGGGGATAC+AGG - chr5.2:74613535-74613554 None:intergenic 70.0%
CCTGTATCCCCGGCAGCAGC+AGG + chr5.2:74613532-74613551 MS.gene016881:CDS 70.0%
TCCCCGGCAGCAGCAGGACA+AGG + chr5.2:74613538-74613557 MS.gene016881:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 74612814 74615357 74612814 ID=MS.gene016881
chr5.2 mRNA 74612814 74615357 74612814 ID=MS.gene016881.t1;Parent=MS.gene016881
chr5.2 exon 74612814 74613005 74612814 ID=MS.gene016881.t1.exon1;Parent=MS.gene016881.t1
chr5.2 CDS 74612814 74613005 74612814 ID=cds.MS.gene016881.t1;Parent=MS.gene016881.t1
chr5.2 exon 74613382 74613755 74613382 ID=MS.gene016881.t1.exon2;Parent=MS.gene016881.t1
chr5.2 CDS 74613382 74613755 74613382 ID=cds.MS.gene016881.t1;Parent=MS.gene016881.t1
chr5.2 exon 74614351 74614647 74614351 ID=MS.gene016881.t1.exon3;Parent=MS.gene016881.t1
chr5.2 CDS 74614351 74614647 74614351 ID=cds.MS.gene016881.t1;Parent=MS.gene016881.t1
chr5.2 exon 74614865 74615357 74614865 ID=MS.gene016881.t1.exon4;Parent=MS.gene016881.t1
chr5.2 CDS 74614865 74615357 74614865 ID=cds.MS.gene016881.t1;Parent=MS.gene016881.t1
Gene Sequence

>MS.gene016881

ATGGTGAGGATCAGAGGAGGAAAGCAGAGGAAAAGTGTTGATGAGAAAAGTGTTGATGTTCCTGTGTTGGTTGTTGGAACCAAGTCTTCGCCAGCTCTTTCTCATGCTCAAACTCCAAAACCTATGAATCATGTTGAGAATGTTTCTGTGCCTAGTGTTGGTCAGGTTGCTCCTAGAGCTCCTTCTGTTCAGGTTCGATTGTTCTTTACTGTAATTCGATAGCATTGTCGTTTGAGAATGAAATGCAAAAGATCATGTTAGTGTAATGTGATTTTGATGGAGTTTTTTTGTTGAGATAAACATGTTTTGTATTGATCTATTTTTCATGATTTTAGCCATATTATTGCAAGCATAAGAATTGATTTTGATAGAGTAACAATTAAAATATGATGTGTCAGAATTAAACAACGAGTGAAAAATATGGCGAGTCGACAAATCTGTTTAGGACATATTGTTTTTTTTCCCTTCGATGGAAATAAAAGCTGTATTCTAGAATATTCTTAACTCTGGGTTTGACTATTTTTTCGTGTTTGCCTGATTTTCCGTGTATGGATCTGTGATTAAACAGAAATCAAACGGTTCTGGAAACTTCAATCCGAAGAAGAAGATGCCATCACCGCGCTATCGAAAGCCAGGCCCCAAGCGCAACAGAAACACAAATGACACGCCTCACTTTCCTGTGGCGACGATGCCTTATCCTAATGGCGAGAATCCTAAGCCTGTATCCCCGGCAGCAGCAGGACAAGGTTTTACTCCACCTGCTCATGCCATTGATGCCAAACATGTTCAGCCTCCGGTGCAAGGAGATCCGAATGCTTATGCTGTTAATTATCCAAATGGAAGGCCAAATATCCAAGAACAAGGTGACCATGTGAATCATGGTCGGCATCATCAAAGACCTTTTCCTGCCAGGGCAAACATGCCTATGCCCTATGCAACACAGTAAAATTTCTAATTAAAAATCCTCCATATATGCACTTCATGCAATTAACCACTTATTCATTTAGCTTAACATTTTTGCTGTTCTTTCACCTTTGAATGAACTTCATTGAGTTTATGTAGCGACTTTTTTGTTACTGTTACTGTTGTTGAAGATTTTAGTCTCGTTTTCTTGATTAAGTTTCAAGATTCTAGCTTAACCCTCTGGTGCTTTTTTGGGTATTGTTTTGTCTTTTATATGATTAATATCACTAATCTTCAATATTCTTACTTTGATACCATTGAAGTGGCTTTTAGTTGCAACATGATGTTTTACGATGTGTGTTTTTTATTGTCATTATGATATCTTCTCGTTATGGTTCTGTAATTGGTTTGAGTAACTGGCCAAGTAATTTTGTTAACTTACATAATTGGCTAGTTCATCTGATTAAGGCATAGTTGATTTGATTAAAGCATCGTTTTTTGAGTAAACCAGGTGAGGCTGGGGACTAAGAATTAAGATGATTTAGCTGGTATCTAACATATTTTTACCATTCAAAAAATATTATGCATGTCGATATAATTCAAATTGGATGAAATTGGTTTTGAATTCTTATAGTTGTATTTTTGGTATAGAAAGCATGGAAGAAAGAAAAAGTATACAATCATCCCTCCTTCAAATTGATTTAAATGAGCCGGCTTCAGTCCAAGATCCCTCTGAGCATAGCATGGAACAACAACAGGAGAAAACTGGCACCGATATCATTGAGTCGGGTGGTGCCATTGCAATTCTAAACTCTAATGATAATAACGTCACAACTCTAAACTCTGAGGAAAATAACTTAATTCAAGCTGAGTCAGTACCAGAAGAAAGTCATGCCCCAAGGAAAGGGCATTGGACAGAGGATGAACACAAGTAAGTATGCAGTCTATGTTAATGTTAACCTATGTTTGAAAGTTATTTTTTTTATTTTATTGAGTTAAATATGTGTTTAGTTCTTATTAAATTACAGATTTTAACATCCATAAAATTATTCTGTTTTTTGACAATTGTTTTCATTTTATCTTTTATACAATTGTTTTCATATTATTTAGTTGAATTTGAATATTTCACATTCATTCTCTATATATAGGTTGTTTTTGAAGGGGATGAAAAAACATGGAAAGGGACGCTGGAAAAATATTTCAAAAGAATTTGTGGTGACAAAAACTCCAACTCAAATTGCTAGCCATGCCCAAAAATACTTTATCCACCAAAATGTGAAAGATATGGAGAAGAAGGAGAAAAAGAGAAAAAGGATTCACGACACAACATTAAACAAGAATGACACATTGGTCACTGTCGTTGTTGATCAAGAAGATGAGATTCCTTCAGTTGAGCTACAAAGTGTGATTCCTTCTCAAGGAATGCAACAGACACAAACACAACAGAATGAGATTTCTCCTATGTTGTGCTTGCTGCCAATCGGTTCTACAATTCCTTCGGTTGAGCAACAAAACGAGCTTACTCCTTTAGTCACTGCTCCTATGGAACAACAACATGAGACTCCTCCTCAAGACACTGTGTTAGGTGATGAAAATCACAAAGTACTAAATTACTCTATCTACTCCTATCTAAGAAACTAA
Protein sequence

>MS.gene016881.t1

MVRIRGGKQRKSVDEKSVDVPVLVVGTKSSPALSHAQTPKPMNHVENVSVPSVGQVAPRAPSVQKSNGSGNFNPKKKMPSPRYRKPGPKRNRNTNDTPHFPVATMPYPNGENPKPVSPAAAGQGFTPPAHAIDAKHVQPPVQGDPNAYAVNYPNGRPNIQEQGDHVNHGRHHQRPFPARANMPMPYATHCIFGIESMEERKSIQSSLLQIDLNEPASVQDPSEHSMEQQQEKTGTDIIESGGAIAILNSNDNNVTTLNSEENNLIQAESVPEESHAPRKGHWTEDEHKLFLKGMKKHGKGRWKNISKEFVVTKTPTQIASHAQKYFIHQNVKDMEKKEKKRKRIHDTTLNKNDTLVTVVVDQEDEIPSVELQSVIPSQGMQQTQTQQNEISPMLCLLPIGSTIPSVEQQNELTPLVTAPMEQQHETPPQDTVLGDENHKVLNYSIYSYLRN