AlfalfaGEDB | MS.gene016900

M. sativa cultivar XinJiangDaYe / MS.gene016900

KEGG: -
GO: GO:0000166 GO:0003674 GO:0003824 GO:0004672 GO:0005488 GO:0005524 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017076 GO:0019538 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036211 GO:0043167 GO:0043168 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0071704 GO:0097159 GO:0097367 GO:0140096 GO:1901265 GO:1901363 GO:1901564
PFAM: PF00069.28

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016900.t1	XP_013442172.1	91.3	69	6	0	1	69	654	722	1.60E-28	134.8

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016900.t1	O64477	68.1	69	22	0	1	69	655	723	1.1e-20	100.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016900.t1	A0A072TFN9	91.3	69	6	0	1	69	654	722	1.2e-28	134.8

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016900.t1	MTR_0280s0040	91.304	69	6	0	1	69	654	722	1.46e-39	137
MS.gene016900.t1	MTR_2g089360	79.710	69	14	0	1	69	656	724	1.48e-33	120
MS.gene016900.t1	MTR_2g089440	65.217	69	24	0	1	69	429	497	5.86e-28	104
MS.gene016900.t1	MTR_2g089440	65.217	69	24	0	1	69	661	729	1.07e-27	103
MS.gene016900.t1	MTR_5g088435	91.304	46	3	1	1	45	1	46	7.54e-24	85.1
MS.gene016900.t1	MTR_1g012550	50.794	63	31	0	1	63	662	724	2.99e-19	80.1
MS.gene016900.t1	MTR_2g073250	49.206	63	32	0	1	63	646	708	3.45e-18	77.0
MS.gene016900.t1	MTR_3g107070	47.619	63	33	0	1	63	634	696	3.81e-18	77.0
MS.gene016900.t1	MTR_6g043510	53.968	63	29	0	1	63	651	713	2.02e-17	74.7
MS.gene016900.t1	MTR_2g080080	47.619	63	33	0	1	63	642	704	2.14e-17	74.7
MS.gene016900.t1	MTR_1g099400	50.000	66	33	0	1	66	684	749	5.15e-17	73.6
MS.gene016900.t1	MTR_8g030500	55.072	69	21	2	2	60	645	713	6.00e-16	70.5
MS.gene016900.t1	MTR_2g103810	44.444	63	35	0	1	63	654	716	8.69e-16	70.1
MS.gene016900.t1	MTR_6g043790	47.619	63	33	0	1	63	656	718	2.80e-15	68.6
MS.gene016900.t1	MTR_2g080100	46.774	62	32	1	3	63	612	673	1.32e-14	66.6
MS.gene016900.t1	MTR_3g072800	53.061	49	23	0	1	49	681	729	1.46e-13	63.9
MS.gene016900.t1	MTR_4g113710	40.580	69	35	2	1	63	269	337	1.77e-13	63.5
MS.gene016900.t1	MTR_2g054870	42.029	69	34	1	1	63	271	339	6.58e-13	62.0
MS.gene016900.t1	MTR_4g114270	59.524	42	17	0	1	42	686	727	3.92e-12	59.7
MS.gene016900.t1	MTR_1g032930	64.103	39	14	0	2	40	660	698	6.90e-12	58.9
MS.gene016900.t1	MTR_3g102450	45.098	51	28	0	1	51	208	258	7.98e-12	58.9
MS.gene016900.t1	MTR_4g094858	48.780	41	21	0	1	41	673	713	1.35e-11	58.2
MS.gene016900.t1	MTR_3g031510	55.263	38	17	0	1	38	225	262	2.56e-11	57.4
MS.gene016900.t1	MTR_3g031610	55.263	38	17	0	1	38	670	707	3.50e-11	57.0
MS.gene016900.t1	MTR_3g102400	55.263	38	17	0	1	38	679	716	3.64e-11	57.0
MS.gene016900.t1	MTR_7g060570	33.333	66	44	0	3	68	504	569	3.89e-11	57.0
MS.gene016900.t1	MTR_3g031580	44.898	49	25	1	1	47	663	711	5.32e-11	56.6
MS.gene016900.t1	MTR_3g031500	44.898	49	25	1	1	47	584	632	5.35e-11	56.6
MS.gene016900.t1	MTR_7g058830	55.263	38	17	0	1	38	674	711	6.60e-11	56.2
MS.gene016900.t1	MTR_4g114250	52.381	42	20	0	1	42	688	729	7.21e-11	56.2
MS.gene016900.t1	MTR_3g031640	52.632	38	18	0	1	38	673	710	7.49e-11	56.2
MS.gene016900.t1	MTR_3g031600	52.632	38	18	0	1	38	673	710	9.85e-11	55.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene016900.t1	AT2G19130	68.116	69	22	0	1	69	655	723	1.57e-27	103
MS.gene016900.t1	AT4G32300	52.381	63	30	0	1	63	654	716	2.66e-19	80.1
MS.gene016900.t1	AT5G24080	48.571	70	36	0	1	70	565	634	1.62e-17	75.1
MS.gene016900.t1	AT5G24080	48.571	70	36	0	1	70	565	634	1.62e-17	75.1
MS.gene016900.t1	AT5G24080	48.571	70	36	0	1	70	692	761	1.76e-17	75.1
MS.gene016900.t1	AT1G34300	50.794	63	31	0	1	63	645	707	1.64e-16	72.4
MS.gene016900.t1	AT4G00340	47.887	71	29	1	1	63	621	691	2.32e-15	68.9
MS.gene016900.t1	AT4G00340	47.887	71	29	1	1	63	642	712	2.32e-15	68.9
MS.gene016900.t1	AT5G35370	68.293	41	13	0	1	41	675	715	7.95e-15	67.4
MS.gene016900.t1	AT4G04570	46.939	49	26	0	3	51	512	560	6.73e-11	56.2
MS.gene016900.t1	AT4G04540	46.939	49	26	0	3	51	517	565	9.86e-11	55.8
MS.gene016900.t1	AT4G04540	46.939	49	26	0	3	51	488	536	9.94e-11	55.8

Find 21 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGCTCCAGAGTGGATTTC+AGG	0.230728	5.2:+74979376	MS.gene016900:CDS
TGCTAGATTCTTTCCTACTT+TGG	0.365713	5.2:+74979498	MS.gene016900:CDS
GAATCTAGCATGGCCATCTT+CGG	0.469678	5.2:-74979485	None:intergenic
GTTGTAAACATCGGCTTTAG+CGG	0.485838	5.2:-74979410	None:intergenic
AACTCCGATCCTTCCGAAGA+TGG	0.489207	5.2:+74979472	MS.gene016900:CDS
ATAGCCTCTTGTTCCTCTCA+TGG	0.491477	5.2:-74979353	None:intergenic
TAGCCTCTTGTTCCTCTCAT+GGG	0.508954	5.2:-74979352	None:intergenic
GGCTAATCACATTCCCACCT+TGG	0.517860	5.2:-74979537	None:intergenic
ACTGAGTTTGCAGCCAAAGT+AGG	0.545150	5.2:-74979511	None:intergenic
ATGGCCATCTTCGGAAGGAT+CGG	0.564859	5.2:-74979476	None:intergenic
GGTCCCCTCACTCCCATGAG+AGG	0.594449	5.2:+74979340	None:intergenic
GAAACTCCTGAAATCCACTC+TGG	0.599307	5.2:-74979382	None:intergenic
AAAGCCGATGTTTACAACTA+CGG	0.603994	5.2:+74979415	MS.gene016900:CDS
CTAGCATGGCCATCTTCGGA+AGG	0.610392	5.2:-74979481	None:intergenic
CAAACTCAGTACACCAAGGT+GGG	0.634731	5.2:+74979524	MS.gene016900:CDS
CATTCCGTAGTTGTAAACAT+CGG	0.636880	5.2:-74979419	None:intergenic
ACTCCCATGAGAGGAACAAG+AGG	0.658194	5.2:+74979349	None:intergenic
AAGTAGGAAAGAATCTAGCA+TGG	0.658240	5.2:-74979495	None:intergenic
GAGGCTATCTAGCTCCAGAG+TGG	0.676111	5.2:+74979368	MS.gene016900:CDS
GCAAACTCAGTACACCAAGG+TGG	0.702721	5.2:+74979523	MS.gene016900:CDS
GCTGCAAACTCAGTACACCA+AGG	0.759095	5.2:+74979520	MS.gene016900:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAAGCCGATGTTTACAACTA+CGG	+	chr5.2:74979415-74979434	MS.gene016900:CDS	35.0%
	AAGTAGGAAAGAATCTAGCA+TGG	-	chr5.2:74979498-74979517	None:intergenic	35.0%
	CATTCCGTAGTTGTAAACAT+CGG	-	chr5.2:74979422-74979441	None:intergenic	35.0%
	TGCTAGATTCTTTCCTACTT+TGG	+	chr5.2:74979498-74979517	MS.gene016900:CDS	35.0%
!!	GTTGTAAACATCGGCTTTAG+CGG	-	chr5.2:74979413-74979432	None:intergenic	40.0%
	ACTGAGTTTGCAGCCAAAGT+AGG	-	chr5.2:74979514-74979533	None:intergenic	45.0%
	CAAACTCAGTACACCAAGGT+GGG	+	chr5.2:74979524-74979543	MS.gene016900:CDS	45.0%
	GAAACTCCTGAAATCCACTC+TGG	-	chr5.2:74979385-74979404	None:intergenic	45.0%
	GAATCTAGCATGGCCATCTT+CGG	-	chr5.2:74979488-74979507	None:intergenic	45.0%
	AACTCCGATCCTTCCGAAGA+TGG	+	chr5.2:74979472-74979491	MS.gene016900:CDS	50.0%
	ATGGCCATCTTCGGAAGGAT+CGG	-	chr5.2:74979479-74979498	None:intergenic	50.0%
	GCAAACTCAGTACACCAAGG+TGG	+	chr5.2:74979523-74979542	MS.gene016900:CDS	50.0%
	GCTGCAAACTCAGTACACCA+AGG	+	chr5.2:74979520-74979539	MS.gene016900:CDS	50.0%
	GGCTAATCACATTCCCACCT+TGG	-	chr5.2:74979540-74979559	None:intergenic	50.0%
!	CTAGCTCCAGAGTGGATTTC+AGG	+	chr5.2:74979376-74979395	MS.gene016900:CDS	50.0%
	CTAGCATGGCCATCTTCGGA+AGG	-	chr5.2:74979484-74979503	None:intergenic	55.0%
!	ATGCTTTTCGCGCTCGTGTC+AGG	+	chr5.2:74979442-74979461	MS.gene016900:CDS	55.0%
!	GAGGCTATCTAGCTCCAGAG+TGG	+	chr5.2:74979368-74979387	MS.gene016900:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	74979355	74979576	74979355	ID=MS.gene016900
chr5.2	mRNA	74979355	74979576	74979355	ID=MS.gene016900.t1;Parent=MS.gene016900
chr5.2	exon	74979355	74979576	74979355	ID=MS.gene016900.t1.exon1;Parent=MS.gene016900.t1
chr5.2	CDS	74979355	74979576	74979355	ID=cds.MS.gene016900.t1;Parent=MS.gene016900.t1

Gene Sequence	>MS.gene016900 ATGAGAGGAACAAGAGGCTATCTAGCTCCAGAGTGGATTTCAGGAGTTTCTATGACCGCTAAAGCCGATGTTTACAACTACGGAATGATGCTTTTCGCGCTCGTGTCAGGAAGAAGAAACTCCGATCCTTCCGAAGATGGCCATGCTAGATTCTTTCCTACTTTGGCTGCAAACTCAGTACACCAAGGTGGGAATGTGATTAGCCTTAATAATGTCAAATGA
Protein sequence	>MS.gene016900.t1 MRGTRGYLAPEWISGVSMTAKADVYNYGMMLFALVSGRRNSDPSEDGHARFFPTLAANSVHQGGNVISLNNVK