AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016988

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016988.t1 MTR_5g089750 96.646 328 4 3 22 348 146 467 0.0 645
MS.gene016988.t1 MTR_3g014660 42.949 312 142 9 30 332 45 329 1.08e-67 216
MS.gene016988.t1 MTR_8g039110 41.414 297 135 10 35 316 42 314 7.04e-60 196
MS.gene016988.t1 MTR_8g076620 44.865 185 76 5 101 285 94 252 4.50e-43 152
MS.gene016988.t1 MTR_5g021130 43.379 219 105 6 124 338 93 296 1.21e-40 145
MS.gene016988.t1 MTR_3g112510 40.761 184 91 4 121 302 76 243 2.87e-33 125
MS.gene016988.t1 MTR_4g071110 39.216 153 61 3 131 283 17 137 6.57e-28 110
MS.gene016988.t1 MTR_0363s0040 55.172 87 31 2 121 205 114 194 1.11e-25 103
MS.gene016988.t1 MTR_1g023230 86.667 45 6 0 131 175 122 166 2.68e-20 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016988.t1 AT5G12330 63.592 206 57 6 131 327 112 308 1.79e-79 246
MS.gene016988.t1 AT5G12330 63.592 206 57 6 131 327 112 308 1.79e-79 246
MS.gene016988.t1 AT5G12330 51.645 304 98 8 38 327 127 395 6.13e-79 247
MS.gene016988.t1 AT5G66350 50.000 158 65 3 131 287 120 264 1.10e-43 153
MS.gene016988.t1 AT5G66350 50.000 158 65 3 131 287 120 264 1.87e-43 153
MS.gene016988.t1 AT5G66350 50.000 158 65 3 131 287 120 264 1.93e-43 153
MS.gene016988.t1 AT1G75520 45.506 178 86 5 119 288 113 287 5.75e-43 152
MS.gene016988.t1 AT1G19790 37.500 264 108 8 71 283 12 269 6.29e-42 149
MS.gene016988.t1 AT1G19790 37.500 264 108 8 71 283 12 269 6.29e-42 149
MS.gene016988.t1 AT4G36260 41.841 239 103 6 54 285 37 246 3.01e-41 147
MS.gene016988.t1 AT3G51060 47.500 160 58 4 129 281 142 282 4.78e-41 147
MS.gene016988.t1 AT2G18120 44.643 168 71 2 122 289 63 208 6.92e-39 138
MS.gene016988.t1 AT2G18120 44.643 168 73 2 122 289 63 210 1.15e-38 137
MS.gene016988.t1 AT3G54430 42.353 170 73 3 127 295 37 182 1.85e-37 133
MS.gene016988.t1 AT2G21400 40.881 159 71 2 131 289 10 145 1.21e-34 125
MS.gene016988.t1 AT2G21400 40.881 159 71 2 131 289 9 144 1.37e-34 125
MS.gene016988.t1 AT2G21400 40.000 160 56 2 131 289 10 130 2.10e-33 122
MS.gene016988.t1 AT2G21400 40.000 160 56 2 131 289 9 129 2.39e-33 122
MS.gene016988.t1 AT5G12330 64.762 105 29 3 131 229 112 214 9.53e-31 116
MS.gene016988.t1 AT5G33210 72.222 54 15 0 129 182 15 68 2.53e-21 87.8
MS.gene016988.t1 AT5G33210 72.222 54 15 0 129 182 50 103 6.23e-21 89.0

Find 102 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATACAAGTCCAACAAATT+TGG 0.195666 5.2:-76140176 MS.gene016988:CDS
CATGGTACTGGTGGGTTAAC+TGG 0.215140 5.2:-76140151 MS.gene016988:CDS
GGTGGAAGCTCCGGCGTTTC+CGG 0.252237 5.2:+76140561 None:intergenic
GGTTCTAGTGGTTCAACTTC+TGG 0.271027 5.2:-76139920 MS.gene016988:CDS
CCGCCACCGCTCTCGGTGTC+GGG 0.292941 5.2:-76140384 MS.gene016988:CDS
TTACTTGGAGGTTCAGCTTA+TGG 0.298507 5.2:-76138891 MS.gene016988:CDS
ACCAACAAGGAATTCAGTTT+TGG 0.307252 5.2:-76140297 MS.gene016988:CDS
TACTACAATCTTTCTTTGCT+TGG 0.312295 5.2:+76140077 None:intergenic
TGATCATGGTGTTGAGAATA+GGG 0.324275 5.2:-76139060 MS.gene016988:CDS
AGCTCCGGCGTTTCCGGCGG+TGG 0.340870 5.2:+76140567 None:intergenic
ACTGGTGGGTTAACTGGTTC+TGG 0.346294 5.2:-76140145 MS.gene016988:CDS
AACCTTTCTGAGCTTCATTT+AGG 0.352612 5.2:-76139026 MS.gene016988:CDS
GCCAAAACTGAATTCCTTGT+TGG 0.364262 5.2:+76140296 None:intergenic
AGTGGTGGTGGAGGGTTACT+TGG 0.368806 5.2:-76138906 MS.gene016988:CDS
ATGATCATGGTGTTGAGAAT+AGG 0.379627 5.2:-76139061 MS.gene016988:CDS
CTACTCATGTTAAGAGTACT+TGG 0.387799 5.2:-76140009 MS.gene016988:CDS
AGAAAGATTGTAGTAATAGA+AGG 0.391384 5.2:-76140069 MS.gene016988:CDS
AGTCCAACAAATTTGGTTCA+TGG 0.393800 5.2:-76140169 MS.gene016988:CDS
TGAGAATTAACAAACCTTGA+TGG 0.401434 5.2:+76139804 None:intergenic
GGTGGTGGTGGTAATAATAA+TGG 0.402915 5.2:-76139005 MS.gene016988:CDS
GCGACAACGACGGTGGTTGC+TGG 0.403885 5.2:-76139947 MS.gene016988:CDS
CCCGACACCGAGAGCGGTGG+CGG 0.404701 5.2:+76140384 None:intergenic
GAGGTAGTATCAAAGCTTCT+AGG 0.414831 5.2:+76139830 None:intergenic
CTTAACCCCGCCACCGCTCT+CGG 0.417771 5.2:-76140391 MS.gene016988:CDS
GTACCATGAACCAAATTTGT+TGG 0.418091 5.2:+76140166 None:intergenic
CCCGCCACCGCTCTCGGTGT+CGG 0.421375 5.2:-76140385 MS.gene016988:CDS
ACAAATTTGGTTCATGGTAC+TGG 0.423871 5.2:-76140163 MS.gene016988:CDS
GGAGGGTCCGGTGGAAGCTC+CGG 0.433703 5.2:+76140552 None:intergenic
TTCAATGCGGGTCCCACCGC+CGG 0.436323 5.2:-76140580 None:intergenic
GCAGCTTCAAGTGGTGGTGG+AGG 0.442810 5.2:-76138915 MS.gene016988:CDS
AATGGCGGTGCTAATAGAAA+TGG 0.444064 5.2:-76138987 MS.gene016988:CDS
TGTTGTTCCTCCACCACTAC+TGG 0.460746 5.2:+76140120 None:intergenic
ATATAATCCGGTGGAGGGTC+CGG 0.467301 5.2:+76140540 None:intergenic
TCTGAGCTTCATTTAGGTGG+TGG 0.469113 5.2:-76139020 MS.gene016988:CDS
GAGCTTCATTTAGGTGGTGG+TGG 0.470751 5.2:-76139017 MS.gene016988:CDS
GGTGGTGGAGGGTTACTTGG+AGG 0.474597 5.2:-76138903 MS.gene016988:CDS
AAATGAAGCTCAGAAAGGTT+AGG 0.477510 5.2:+76139029 None:intergenic
GATACTACCTCTAGCCATCA+AGG 0.481688 5.2:-76139818 MS.gene016988:intron
CCGGATTATATAATTCATCT+AGG 0.485408 5.2:-76140528 MS.gene016988:intron
GTTTGTGAAGCAATAAGTCT+AGG 0.489243 5.2:+76139887 None:intergenic
GTCTTCTTCATCACCAATGA+TGG 0.489923 5.2:-76138961 MS.gene016988:CDS
GGTTTCAAAGATTCAATGCC+GGG 0.492328 5.2:-76139206 MS.gene016988:CDS
GGGTACCTGCTGCTAGAAGA+AGG 0.496203 5.2:-76139988 MS.gene016988:CDS
CAAGTACTCTTAACATGAGT+AGG 0.499272 5.2:+76140010 None:intergenic
TCATGAGAAGGATCCATCAT+TGG 0.499688 5.2:+76138948 None:intergenic
CTTTCTGAGCTTCATTTAGG+TGG 0.503901 5.2:-76139023 MS.gene016988:CDS
GTTGCTGGTGGTGGTTCTAG+TGG 0.506006 5.2:-76139932 MS.gene016988:CDS
CGTTCCCTTCTTCTAGCAGC+AGG 0.506124 5.2:+76139983 None:intergenic
CCGACACCGAGAGCGGTGGC+GGG 0.513284 5.2:+76140385 None:intergenic
CCTAGATGAATTATATAATC+CGG 0.516389 5.2:+76140528 None:intergenic
ATTGTAGTAATAGAAGGTGT+AGG 0.521083 5.2:-76140063 MS.gene016988:CDS
GGTCCCACCGCCGGAAACGC+CGG 0.524097 5.2:-76140571 MS.gene016988:CDS
GCTCCGGCGTTTCCGGCGGT+GGG 0.525360 5.2:+76140568 None:intergenic
ACGACGGTGGTTGCTGGTGG+TGG 0.527037 5.2:-76139941 MS.gene016988:CDS
CATGGAGAAGCTGTGAGTAA+AGG 0.534475 5.2:+76140355 None:intergenic
GCAGCAGCTTCAAGTGGTGG+TGG 0.540984 5.2:-76138918 MS.gene016988:CDS
GCTTCCACCGGACCCTCCAC+CGG 0.545726 5.2:-76140547 MS.gene016988:CDS
ACTGGTTCTGGTACCAGTAG+TGG 0.546183 5.2:-76140133 MS.gene016988:CDS
AATTTGGTTCATGGTACTGG+TGG 0.555469 5.2:-76140160 MS.gene016988:CDS
ACAACAACGTGTCAAGATTG+TGG 0.558898 5.2:-76140103 MS.gene016988:CDS
TGAATTATATAATCCGGTGG+AGG 0.559774 5.2:+76140534 None:intergenic
AGGTTTCAAAGATTCAATGC+CGG 0.561816 5.2:-76139207 MS.gene016988:CDS
CACCTAAATGAAGCTCAGAA+AGG 0.565023 5.2:+76139024 None:intergenic
GCCGGGTCAAGTACGTGCAC+CGG 0.570539 5.2:-76139189 MS.gene016988:CDS
CGCCACCGCTCTCGGTGTCG+GGG 0.570886 5.2:-76140383 MS.gene016988:CDS
AGCAACCACCGTCGTTGTCG+CGG 0.571590 5.2:+76139949 None:intergenic
AGGACTTGTTGTAAAAGTAG+AGG 0.575268 5.2:-76140043 MS.gene016988:CDS
TATCAAGCTGTAGTGAAGAT+TGG 0.579339 5.2:-76139110 MS.gene016988:CDS
TTCTTCGACCGCGACAACGA+CGG 0.583043 5.2:-76139957 MS.gene016988:CDS
GGAAACGCCGGAGCTTCCAC+CGG 0.586967 5.2:-76140559 MS.gene016988:CDS
AAAGAAAACCTTTGAAAACA+TGG 0.590040 5.2:+76139084 None:intergenic
CTAACACACTTGAATAGAGC+CGG 0.591611 5.2:+76139170 None:intergenic
TCTGGTACCAGTAGTGGTGG+AGG 0.592565 5.2:-76140127 MS.gene016988:CDS
CGACACCGAGAGCGGTGGCG+GGG 0.592680 5.2:+76140386 None:intergenic
ATTTGGTTCATGGTACTGGT+GGG 0.594801 5.2:-76140159 MS.gene016988:CDS
GGAAGCTCCGGCGTTTCCGG+CGG 0.598478 5.2:+76140564 None:intergenic
AGTATCAAAGCTTCTAGGAG+GGG 0.599007 5.2:+76139835 None:intergenic
AAGTACTCTTAACATGAGTA+GGG 0.604249 5.2:+76140011 None:intergenic
CAAGCTGTAGTGAAGATTGG+TGG 0.605202 5.2:-76139107 MS.gene016988:CDS
TAATCCGGTGGAGGGTCCGG+TGG 0.607835 5.2:+76140543 None:intergenic
CAGCTTCAAGTGGTGGTGGA+GGG 0.610373 5.2:-76138914 MS.gene016988:CDS
GCCGGTGCACGTACTTGACC+CGG 0.610717 5.2:+76139188 None:intergenic
TATGCAGCAGCTTCAAGTGG+TGG 0.610824 5.2:-76138921 MS.gene016988:CDS
GTAGTATCAAAGCTTCTAGG+AGG 0.611169 5.2:+76139833 None:intergenic
TAGTATCAAAGCTTCTAGGA+GGG 0.613507 5.2:+76139834 None:intergenic
GGTTCTGGTACCAGTAGTGG+TGG 0.613955 5.2:-76140130 MS.gene016988:CDS
AGAGTGACATCAGTGGATGA+TGG 0.614276 5.2:-76139149 MS.gene016988:CDS
GGTACCTGCTGCTAGAAGAA+GGG 0.616105 5.2:-76139987 MS.gene016988:CDS
TAACAAACCTTGATGGCTAG+AGG 0.619709 5.2:+76139811 None:intergenic
TTCGACCGCGACAACGACGG+TGG 0.620852 5.2:-76139954 MS.gene016988:CDS
GAATTATATAATCCGGTGGA+GGG 0.620952 5.2:+76140535 None:intergenic
TACTCATGTTAAGAGTACTT+GGG 0.637418 5.2:-76140008 MS.gene016988:CDS
GCTGCATAAACATCATGAGA+AGG 0.637740 5.2:+76138936 None:intergenic
AACCCCGACACCGAGAGCGG+TGG 0.656167 5.2:+76140381 None:intergenic
GTGGCGGGGTTAAGAGAGTG+AGG 0.668996 5.2:+76140400 None:intergenic
ACAACGACGGTGGTTGCTGG+TGG 0.669044 5.2:-76139944 MS.gene016988:CDS
GTTTATGCAGCAGCTTCAAG+TGG 0.670690 5.2:-76138924 MS.gene016988:CDS
TATAACCCCGACACCGAGAG+CGG 0.681070 5.2:+76140378 None:intergenic
AGATGAATTATATAATCCGG+TGG 0.684484 5.2:+76140531 None:intergenic
GTGTGTTAGAGTGACATCAG+TGG 0.704487 5.2:-76139156 MS.gene016988:CDS
GGTGGTGGTAATAATAATGG+CGG 0.719095 5.2:-76139002 MS.gene016988:CDS
AACAATAGAAGAAACCAACA+AGG 0.735803 5.2:-76140310 MS.gene016988:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAGTTAGTTTAATTTTTT+GGG - chr5.2:76140158-76140177 MS.gene016988:CDS 10.0%
!! AACCTAAAATTATATTATGA+AGG + chr5.2:76139688-76139707 None:intergenic 15.0%
!! TAAAAATTAGTTAAACAAGA+TGG + chr5.2:76139946-76139965 None:intergenic 15.0%
!!! CTTTAGTTAGTTTAATTTTT+TGG - chr5.2:76140157-76140176 MS.gene016988:CDS 15.0%
!!! TTCCTTCATAATATAATTTT+AGG - chr5.2:76139683-76139702 MS.gene016988:intron 15.0%
!! AAATAAAAATAGTGCAAGTT+TGG - chr5.2:76139725-76139744 MS.gene016988:intron 20.0%
!!! TTTTGTTGATAAAATCACAA+TGG - chr5.2:76139798-76139817 MS.gene016988:intron 20.0%
! AGAAAGATTGTAGTAATAGA+AGG - chr5.2:76139382-76139401 MS.gene016988:intron 25.0%
! CACTATTTGAAAAAACAACA+AGG - chr5.2:76139237-76139256 MS.gene016988:intron 25.0%
! CCTAGATGAATTATATAATC+CGG + chr5.2:76138926-76138945 None:intergenic 25.0%
! TAATACAAGTCCAACAAATT+TGG - chr5.2:76139275-76139294 MS.gene016988:intron 25.0%
!! AAAGAAAACCTTTGAAAACA+TGG + chr5.2:76140370-76140389 None:intergenic 25.0%
!! AAAGGTTTTCTTTATGATCA+TGG - chr5.2:76140377-76140396 MS.gene016988:CDS 25.0%
!!! ATGTTTTCATTTTCAAGACA+TGG + chr5.2:76139117-76139136 None:intergenic 25.0%
!!! TTTTTCAAATAGTGTGAATG+TGG + chr5.2:76139231-76139250 None:intergenic 25.0%
AACAATAGAAGAAACCAACA+AGG - chr5.2:76139141-76139160 MS.gene016988:CDS 30.0%
AACTATAAACTCTGCACAAT+TGG + chr5.2:76139831-76139850 None:intergenic 30.0%
AAGTACTCTTAACATGAGTA+GGG + chr5.2:76139443-76139462 None:intergenic 30.0%
AGATGAATTATATAATCCGG+TGG + chr5.2:76138923-76138942 None:intergenic 30.0%
ATTAATTATGGACCTGAGAT+GGG - chr5.2:76138949-76138968 MS.gene016988:CDS 30.0%
CCGGATTATATAATTCATCT+AGG - chr5.2:76138923-76138942 MS.gene016988:CDS 30.0%
GTAGCTAGTGAGAAAAATAA+TGG + chr5.2:76139892-76139911 None:intergenic 30.0%
TACTACAATCTTTCTTTGCT+TGG + chr5.2:76139377-76139396 None:intergenic 30.0%
TCATCTAGGTCCATTAATTA+TGG - chr5.2:76138937-76138956 MS.gene016988:CDS 30.0%
TGAGAATTAACAAACCTTGA+TGG + chr5.2:76139650-76139669 None:intergenic 30.0%
! AAGATGGTTGATCTTTTCAT+TGG + chr5.2:76139930-76139949 None:intergenic 30.0%
! ATTGTAGTAATAGAAGGTGT+AGG - chr5.2:76139388-76139407 MS.gene016988:intron 30.0%
! TTTTTCTCACTAGCTACATA+AGG - chr5.2:76139895-76139914 MS.gene016988:CDS 30.0%
!! AGAAAAATAATGGACTAGTG+TGG + chr5.2:76139882-76139901 None:intergenic 30.0%
!! TACTCATGTTAAGAGTACTT+GGG - chr5.2:76139443-76139462 MS.gene016988:intron 30.0%
!!! TGAAGAGTTGTGTTTTGATT+TGG - chr5.2:76140199-76140218 MS.gene016988:CDS 30.0%
AAATGAAGCTCAGAAAGGTT+AGG + chr5.2:76140425-76140444 None:intergenic 35.0%
AACCTTTCTGAGCTTCATTT+AGG - chr5.2:76140425-76140444 MS.gene016988:intron 35.0%
AGGTTTCAAAGATTCAATGC+CGG - chr5.2:76140244-76140263 MS.gene016988:CDS 35.0%
CAAGTACTCTTAACATGAGT+AGG + chr5.2:76139444-76139463 None:intergenic 35.0%
CATCTCAGGTCCATAATTAA+TGG + chr5.2:76138950-76138969 None:intergenic 35.0%
CATTAATTATGGACCTGAGA+TGG - chr5.2:76138948-76138967 MS.gene016988:CDS 35.0%
GAATTATATAATCCGGTGGA+GGG + chr5.2:76138919-76138938 None:intergenic 35.0%
GATAAAATCACAATGGCTCA+AGG - chr5.2:76139805-76139824 MS.gene016988:intron 35.0%
GTACCATGAACCAAATTTGT+TGG + chr5.2:76139288-76139307 None:intergenic 35.0%
GTTTGTGAAGCAATAAGTCT+AGG + chr5.2:76139567-76139586 None:intergenic 35.0%
TATCAAGCTGTAGTGAAGAT+TGG - chr5.2:76140341-76140360 MS.gene016988:CDS 35.0%
TGAATTATATAATCCGGTGG+AGG + chr5.2:76138920-76138939 None:intergenic 35.0%
! ACAAATTTGGTTCATGGTAC+TGG - chr5.2:76139288-76139307 MS.gene016988:intron 35.0%
! ACCAACAAGGAATTCAGTTT+TGG - chr5.2:76139154-76139173 MS.gene016988:CDS 35.0%
! AGTCCAACAAATTTGGTTCA+TGG - chr5.2:76139282-76139301 MS.gene016988:intron 35.0%
! ATGATCATGGTGTTGAGAAT+AGG - chr5.2:76140390-76140409 MS.gene016988:CDS 35.0%
! TAGTATCAAAGCTTCTAGGA+GGG + chr5.2:76139620-76139639 None:intergenic 35.0%
! TGATCATGGTGTTGAGAATA+GGG - chr5.2:76140391-76140410 MS.gene016988:CDS 35.0%
!! AGGACTTGTTGTAAAAGTAG+AGG - chr5.2:76139408-76139427 MS.gene016988:intron 35.0%
!! CTACTCATGTTAAGAGTACT+TGG - chr5.2:76139442-76139461 MS.gene016988:intron 35.0%
ACAACAAACATACCCATCTC+AGG + chr5.2:76138964-76138983 None:intergenic 40.0%
ACAACAACGTGTCAAGATTG+TGG - chr5.2:76139348-76139367 MS.gene016988:intron 40.0%
CACCTAAATGAAGCTCAGAA+AGG + chr5.2:76140430-76140449 None:intergenic 40.0%
CTAACACACTTGAATAGAGC+CGG + chr5.2:76140284-76140303 None:intergenic 40.0%
CTGCTTCATGATTGTTATGG+TGG + chr5.2:76139017-76139036 None:intergenic 40.0%
CTTCATGATTGTTATGGTGG+TGG + chr5.2:76139014-76139033 None:intergenic 40.0%
CTTTCTGAGCTTCATTTAGG+TGG - chr5.2:76140428-76140447 MS.gene016988:intron 40.0%
GAGCTGCTTCATGATTGTTA+TGG + chr5.2:76139020-76139039 None:intergenic 40.0%
GAGGTAGTATCAAAGCTTCT+AGG + chr5.2:76139624-76139643 None:intergenic 40.0%
GCCAAAACTGAATTCCTTGT+TGG + chr5.2:76139158-76139177 None:intergenic 40.0%
GCTGCATAAACATCATGAGA+AGG + chr5.2:76140518-76140537 None:intergenic 40.0%
GGTTTCAAAGATTCAATGCC+GGG - chr5.2:76140245-76140264 MS.gene016988:CDS 40.0%
GTAGTATCAAAGCTTCTAGG+AGG + chr5.2:76139621-76139640 None:intergenic 40.0%
GTATTAATGTGTGCAGATGC+AGG - chr5.2:76140224-76140243 MS.gene016988:CDS 40.0%
GTCTTCTTCATCACCAATGA+TGG - chr5.2:76140490-76140509 MS.gene016988:intron 40.0%
TCATGAGAAGGATCCATCAT+TGG + chr5.2:76140506-76140525 None:intergenic 40.0%
TTACTTGGAGGTTCAGCTTA+TGG - chr5.2:76140560-76140579 MS.gene016988:CDS 40.0%
! AATGGCGGTGCTAATAGAAA+TGG - chr5.2:76140464-76140483 MS.gene016988:intron 40.0%
! AATTTGGTTCATGGTACTGG+TGG - chr5.2:76139291-76139310 MS.gene016988:intron 40.0%
! AGTATCAAAGCTTCTAGGAG+GGG + chr5.2:76139619-76139638 None:intergenic 40.0%
! GGTGGTGGTAATAATAATGG+CGG - chr5.2:76140449-76140468 MS.gene016988:intron 40.0%
! TAACAAACCTTGATGGCTAG+AGG + chr5.2:76139643-76139662 None:intergenic 40.0%
! TGGTGGTGATGAAAAGAAGA+TGG + chr5.2:76138991-76139010 None:intergenic 40.0%
!! ATTGGTGGCCATGTTTTCAA+AGG - chr5.2:76140359-76140378 MS.gene016988:CDS 40.0%
!! ATTTGGTTCATGGTACTGGT+GGG - chr5.2:76139292-76139311 MS.gene016988:intron 40.0%
!! GGTGGTGGTGGTAATAATAA+TGG - chr5.2:76140446-76140465 MS.gene016988:intron 40.0%
AGAGTGACATCAGTGGATGA+TGG - chr5.2:76140302-76140321 MS.gene016988:CDS 45.0%
CAAGCTGTAGTGAAGATTGG+TGG - chr5.2:76140344-76140363 MS.gene016988:CDS 45.0%
CATGGAGAAGCTGTGAGTAA+AGG + chr5.2:76139099-76139118 None:intergenic 45.0%
GATACTACCTCTAGCCATCA+AGG - chr5.2:76139633-76139652 MS.gene016988:intron 45.0%
GTGTGGCTAACTAGTATAGC+TGG + chr5.2:76139865-76139884 None:intergenic 45.0%
GTGTGTTAGAGTGACATCAG+TGG - chr5.2:76140295-76140314 MS.gene016988:CDS 45.0%
GTTTATGCAGCAGCTTCAAG+TGG - chr5.2:76140527-76140546 MS.gene016988:intron 45.0%
TCTGAGCTTCATTTAGGTGG+TGG - chr5.2:76140431-76140450 MS.gene016988:intron 45.0%
! GGTTCTAGTGGTTCAACTTC+TGG - chr5.2:76139531-76139550 MS.gene016988:intron 45.0%
!! CATGATTGTTATGGTGGTGG+TGG + chr5.2:76139011-76139030 None:intergenic 45.0%
ATATAATCCGGTGGAGGGTC+CGG + chr5.2:76138914-76138933 None:intergenic 50.0%
GGTACCTGCTGCTAGAAGAA+GGG - chr5.2:76139464-76139483 MS.gene016988:intron 50.0%
TATGCAGCAGCTTCAAGTGG+TGG - chr5.2:76140530-76140549 MS.gene016988:CDS 50.0%
TGTTGTTCCTCCACCACTAC+TGG + chr5.2:76139334-76139353 None:intergenic 50.0%
! GAGCTTCATTTAGGTGGTGG+TGG - chr5.2:76140434-76140453 MS.gene016988:intron 50.0%
!! ACTGGTGGGTTAACTGGTTC+TGG - chr5.2:76139306-76139325 MS.gene016988:intron 50.0%
!! ACTGGTTCTGGTACCAGTAG+TGG - chr5.2:76139318-76139337 MS.gene016988:intron 50.0%
!! CATGGTACTGGTGGGTTAAC+TGG - chr5.2:76139300-76139319 MS.gene016988:intron 50.0%
!! GATTGTTATGGTGGTGGTGG+TGG + chr5.2:76139008-76139027 None:intergenic 50.0%
CGTTCCCTTCTTCTAGCAGC+AGG + chr5.2:76139471-76139490 None:intergenic 55.0%
GGGTACCTGCTGCTAGAAGA+AGG - chr5.2:76139463-76139482 MS.gene016988:intron 55.0%
TATAACCCCGACACCGAGAG+CGG + chr5.2:76139076-76139095 None:intergenic 55.0%
TCTGGTACCAGTAGTGGTGG+AGG - chr5.2:76139324-76139343 MS.gene016988:intron 55.0%
TTCTTCGACCGCGACAACGA+CGG - chr5.2:76139494-76139513 MS.gene016988:intron 55.0%
! GGTTCTGGTACCAGTAGTGG+TGG - chr5.2:76139321-76139340 MS.gene016988:intron 55.0%
!! AGTGGTGGTGGAGGGTTACT+TGG - chr5.2:76140545-76140564 MS.gene016988:CDS 55.0%
!! CAGCTTCAAGTGGTGGTGGA+GGG - chr5.2:76140537-76140556 MS.gene016988:CDS 55.0%
!! GTTGCTGGTGGTGGTTCTAG+TGG - chr5.2:76139519-76139538 MS.gene016988:intron 55.0%
ACAACGACGGTGGTTGCTGG+TGG - chr5.2:76139507-76139526 MS.gene016988:intron 60.0%
AGCAACCACCGTCGTTGTCG+CGG + chr5.2:76139505-76139524 None:intergenic 60.0%
GCAGCAGCTTCAAGTGGTGG+TGG - chr5.2:76140533-76140552 MS.gene016988:CDS 60.0%
! GTGGCGGGGTTAAGAGAGTG+AGG + chr5.2:76139054-76139073 None:intergenic 60.0%
!! GCAGCTTCAAGTGGTGGTGG+AGG - chr5.2:76140536-76140555 MS.gene016988:CDS 60.0%
!! GGTGGTGGAGGGTTACTTGG+AGG - chr5.2:76140548-76140567 MS.gene016988:CDS 60.0%
ACGACGGTGGTTGCTGGTGG+TGG - chr5.2:76139510-76139529 MS.gene016988:intron 65.0%
CTTAACCCCGCCACCGCTCT+CGG - chr5.2:76139060-76139079 MS.gene016988:CDS 65.0%
GCCGGGTCAAGTACGTGCAC+CGG - chr5.2:76140262-76140281 MS.gene016988:CDS 65.0%
GCCGGTGCACGTACTTGACC+CGG + chr5.2:76140266-76140285 None:intergenic 65.0%
GCGACAACGACGGTGGTTGC+TGG - chr5.2:76139504-76139523 MS.gene016988:intron 65.0%
GGAAACGCCGGAGCTTCCAC+CGG - chr5.2:76138892-76138911 MS.gene016988:CDS 65.0%
GGTGGAAGCTCCGGCGTTTC+CGG + chr5.2:76138893-76138912 None:intergenic 65.0%
TAATCCGGTGGAGGGTCCGG+TGG + chr5.2:76138911-76138930 None:intergenic 65.0%
TTCGACCGCGACAACGACGG+TGG - chr5.2:76139497-76139516 MS.gene016988:intron 65.0%
AACCCCGACACCGAGAGCGG+TGG + chr5.2:76139073-76139092 None:intergenic 70.0%
GCTTCCACCGGACCCTCCAC+CGG - chr5.2:76138904-76138923 MS.gene016988:CDS 70.0%
GGAAGCTCCGGCGTTTCCGG+CGG + chr5.2:76138890-76138909 None:intergenic 70.0%
GGAGGGTCCGGTGGAAGCTC+CGG + chr5.2:76138902-76138921 None:intergenic 70.0%
AGCTCCGGCGTTTCCGGCGG+TGG + chr5.2:76138887-76138906 None:intergenic 75.0%
CCCGACACCGAGAGCGGTGG+CGG + chr5.2:76139070-76139089 None:intergenic 75.0%
CCGACACCGAGAGCGGTGGC+GGG + chr5.2:76139069-76139088 None:intergenic 75.0%
CGACACCGAGAGCGGTGGCG+GGG + chr5.2:76139068-76139087 None:intergenic 75.0%
GCTCCGGCGTTTCCGGCGGT+GGG + chr5.2:76138886-76138905 None:intergenic 75.0%
GGTCCCACCGCCGGAAACGC+CGG - chr5.2:76138880-76138899 MS.gene016988:CDS 75.0%
! CCCGCCACCGCTCTCGGTGT+CGG - chr5.2:76139066-76139085 MS.gene016988:CDS 75.0%
!! CCGCCACCGCTCTCGGTGTC+GGG - chr5.2:76139067-76139086 MS.gene016988:CDS 75.0%
!! CGCCACCGCTCTCGGTGTCG+GGG - chr5.2:76139068-76139087 MS.gene016988:CDS 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 76138875 76140598 76138875 ID=MS.gene016988
chr5.2 mRNA 76138875 76140598 76138875 ID=MS.gene016988.t1;Parent=MS.gene016988
chr5.2 exon 76140529 76140598 76140529 ID=MS.gene016988.t1.exon1;Parent=MS.gene016988.t1
chr5.2 CDS 76140529 76140598 76140529 ID=cds.MS.gene016988.t1;Parent=MS.gene016988.t1
chr5.2 exon 76139819 76140436 76139819 ID=MS.gene016988.t1.exon2;Parent=MS.gene016988.t1
chr5.2 CDS 76139819 76140436 76139819 ID=cds.MS.gene016988.t1;Parent=MS.gene016988.t1
chr5.2 exon 76138875 76139233 76138875 ID=MS.gene016988.t1.exon3;Parent=MS.gene016988.t1
chr5.2 CDS 76138875 76139233 76138875 ID=cds.MS.gene016988.t1;Parent=MS.gene016988.t1
Gene Sequence

>MS.gene016988

ATGCGGGTCCCACCGCCGGAAACGCCGGAGCTTCCACCGGACCCTCCACCGGATTATATAATTCATCTAGGTCCATTAATTATGGACCTGAGATGGGTATGTTTGTTGTAGCACCATCTTCTTTTCATCACCACCACCACCACCATAACAATCATGAAGCAGCTCTTATGTCTGATCCTCACTCTCTTAACCCCGCCACCGCTCTCGGTGTCGGGGTTATACCTTTACTCACAGCTTCTCCATGTCTTGAAAATGAAAACATGTTAAACAATAGAAGAAACCAACAAGGAATTCAGTTTTGGCATGAACAACATCATCATCATCAACAGCAACAACAACAACAACAACAAAGTCCACATTCACACTATTTGAAAAAACAACAAGGTTTTCTTGATCATCATAATACAAGTCCAACAAATTTGGTTCATGGTACTGGTGGGTTAACTGGTTCTGGTACCAGTAGTGGTGGAGGAACAACAACGTGTCAAGATTGTGGAAACCAAGCAAAGAAAGATTGTAGTAATAGAAGGTGTAGGACTTGTTGTAAAAGTAGAGGTTTTGATTGCCCTACTCATGTTAAGAGTACTTGGGTACCTGCTGCTAGAAGAAGGGAACGTCTTTCTTCGACCGCGACAACGACGGTGGTTGCTGGTGGTGGTTCTAGTGGTTCAACTTCTGGTGCTAAGAAACCTAGACTTATTGCTTCACAAACTACTTCTCATACTTCTACTTCTAATACTACCCCTCCTAGAAGCTTTGATACTACCTCTAGCCATCAAGGTTTGTTAATTCTCATCTTTTTTTTTTTTTCCTTCATAATATAATTTTAGGTTTTTCTAAATGTTACAAAAAATAAAAATAGTGCAAGTTTGGAATCATGATGAGTTTGACAAAATGATATTCTTGTAGCTCAAAGTACTTTTTTTTGTTGATAAAATCACAATGGCTCAAGGCCAATTGTGCAGAGTTTATAGTTAGATGTAAAAGCCAGCTATACTAGTTAGCCACACTAGTCCATTATTTTTCTCACTAGCTACATAAGGCATACATAGCCAATGAAAAGATCAACCATCTTGTTTAACTAATTTTTAACTCTAATTCATTGTCAATCTAAACATACACTATAAACTCTTGCATGCTTTTTCGTAGCGTTGACGATGAAATTATCTTGATCAAAATTAGTTCAAGATTTGATTCTTTAATTAACTTGTTAAACTTGATTCATGAACGAATGAGTTGAGCTCGAGTAATCGACTTGTTTAGTTGATGAAATAGTTTAATACTTTAGTTAGTTTAATTTTTTGGGTTTATTAGTGTGTTAATTTGAAGAGTTGTGTTTTGATTTGGTTGTATTAATGTGTGCAGATGCAGGTTTCAAAGATTCAATGCCGGGTCAAGTACGTGCACCGGCTCTATTCAAGTGTGTTAGAGTGACATCAGTGGATGATGGAAAAGATGAGTATGCATATCAAGCTGTAGTGAAGATTGGTGGCCATGTTTTCAAAGGTTTTCTTTATGATCATGGTGTTGAGAATAGGGAAGTTTATCCTAACCTTTCTGAGCTTCATTTAGGTGGTGGTGGTAATAATAATGGCGGTGCTAATAGAAATGGAGTGTCTTCTTCATCACCAATGATGGATCCTTCTCATGATGTTTATGCAGCAGCTTCAAGTGGTGGTGGAGGGTTACTTGGAGGTTCAGCTTATGGTAATCAAATAAATTGA
Protein sequence

>MS.gene016988.t1

MRVPPPETPELPPDPPPDYIIHLALMSDPHSLNPATALGVGVIPLLTASPCLENENMLNNRRNQQGIQFWHEQHHHHQQQQQQQQQSPHSHYLKKQQGFLDHHNTSPTNLVHGTGGLTGSGTSSGGGTTTCQDCGNQAKKDCSNRRCRTCCKSRGFDCPTHVKSTWVPAARRRERLSSTATTTVVAGGGSSGSTSGAKKPRLIASQTTSHTSTSNTTPPRSFDTTSSHQDAGFKDSMPGQVRAPALFKCVRVTSVDDGKDEYAYQAVVKIGGHVFKGFLYDHGVENREVYPNLSELHLGGGGNNNGGANRNGVSSSSPMMDPSHDVYAAASSGGGGLLGGSAYGNQIN