AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene016989

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene016989.t1 MTR_8g040300 93.750 32 2 0 30 61 113 144 2.78e-14 66.2
MS.gene016989.t1 MTR_8g035890 84.375 32 5 0 30 61 119 150 4.47e-12 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 12 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACATAACATAAACTTATTC+TGG 0.245144 5.2:+76197852 None:intergenic
TGCACAACTTACTTTGTTGC+AGG 0.290633 5.2:-76201567 MS.gene016989:intron
GCGCAAGATGCAATACATTT+CGG 0.421519 5.2:-76201639 None:intergenic
GAACGTACATCTTTGTAAGT+TGG 0.463268 5.2:+76197820 None:intergenic
TATTCTGGTTGCAGTCGTGC+GGG 0.531198 5.2:+76197867 None:intergenic
ACATCTTTGTAAGTTGGAGA+TGG 0.544455 5.2:+76197826 None:intergenic
TTATTCTGGTTGCAGTCGTG+CGG 0.555027 5.2:+76197866 None:intergenic
AAATTTGTTGCTTGATCACG+AGG 0.558311 5.2:-76200641 MS.gene016989:intron
CATTTCGGAGCTAGAACACA+AGG 0.586933 5.2:-76201624 MS.gene016989:CDS
ACACAAGGTTCAGACTCTGC+AGG 0.590651 5.2:-76201609 MS.gene016989:CDS
TGATATCTCTGTGAATAACA+TGG 0.637603 5.2:+76200671 None:intergenic
AATATGTCAATGTTTCCGTG+AGG 0.732618 5.2:+76200704 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTACATCAATATTTATATT+TGG - chr5.2:76199391-76199410 MS.gene016989:intron 10.0%
!! AAAAACAATTATTTCATCTA+TGG + chr5.2:76198952-76198971 None:intergenic 15.0%
!! AAAAACTATTTGCAATTTAT+AGG - chr5.2:76201333-76201352 MS.gene016989:intron 15.0%
!! AAAACAATTATTTCATCTAT+GGG + chr5.2:76198951-76198970 None:intergenic 15.0%
!! AAAACTATTTGCAATTTATA+GGG - chr5.2:76201334-76201353 MS.gene016989:intron 15.0%
!! AAACTTACAAAAATCAATAT+AGG + chr5.2:76198410-76198429 None:intergenic 15.0%
!! AAATGCTTTATATCATTATA+TGG + chr5.2:76201304-76201323 None:intergenic 15.0%
!! AACTTACAAAAATCAATATA+GGG + chr5.2:76198409-76198428 None:intergenic 15.0%
!! AGTAACTCATATTATAATTA+CGG + chr5.2:76200398-76200417 None:intergenic 15.0%
!! CAAATTACTAAAATAAAACA+CGG + chr5.2:76197923-76197942 None:intergenic 15.0%
!!! TTTTTCACTTAATAATAACA+CGG + chr5.2:76200890-76200909 None:intergenic 15.0%
!! AAATTATAAGTAGCATAGAA+TGG + chr5.2:76198627-76198646 None:intergenic 20.0%
!! AACATAACATAAACTTATTC+TGG + chr5.2:76201588-76201607 None:intergenic 20.0%
!! AACTTCAATTTGATTATAAG+CGG - chr5.2:76199339-76199358 MS.gene016989:intron 20.0%
!! ATACACATATTTCAAACTAA+AGG - chr5.2:76198245-76198264 MS.gene016989:intron 20.0%
!! TATATAATCTTACCATCTTA+TGG + chr5.2:76199640-76199659 None:intergenic 20.0%
!!! AAAAATATCGTTCTAAATAG+AGG + chr5.2:76200524-76200543 None:intergenic 20.0%
!!! AAATAGTTTTTGCTTTGAAT+TGG - chr5.2:76199365-76199384 MS.gene016989:intron 20.0%
!!! AATAACAAATTTAAGACAGT+TGG + chr5.2:76200653-76200672 None:intergenic 20.0%
!!! AATAGAAAATAATGTGCTTT+TGG + chr5.2:76201218-76201237 None:intergenic 20.0%
!!! CAATATTTATATTTGGTGAT+TGG - chr5.2:76199398-76199417 MS.gene016989:intron 20.0%
!!! GAAAATATGTTTCTAAATAG+CGG + chr5.2:76200916-76200935 None:intergenic 20.0%
!!! GAATTTTTAGATTATGCATT+TGG - chr5.2:76200081-76200100 MS.gene016989:intron 20.0%
!!! TTATAGTTTTACTTAAATGC+AGG - chr5.2:76201537-76201556 MS.gene016989:intron 20.0%
! AATCATCAAGCATATCATAA+AGG - chr5.2:76200976-76200995 MS.gene016989:intron 25.0%
! ATACAAAGAATTGCTACAAA+AGG + chr5.2:76198658-76198677 None:intergenic 25.0%
! ATTGAAAGTAGTTCACTAAT+AGG - chr5.2:76199291-76199310 MS.gene016989:intron 25.0%
! CTTATCATTGATCAAACTTT+GGG - chr5.2:76199560-76199579 MS.gene016989:intron 25.0%
! TAATGACATGCTATGAAATA+TGG - chr5.2:76198536-76198555 MS.gene016989:intron 25.0%
! TCTTATCATTGATCAAACTT+TGG - chr5.2:76199559-76199578 MS.gene016989:intron 25.0%
! TTGAAAAATAACAACACAAC+AGG + chr5.2:76200833-76200852 None:intergenic 25.0%
!! AAGGTATATCTAAGAAGTAT+TGG + chr5.2:76198170-76198189 None:intergenic 25.0%
!! ATGCTTGATGATTATAATCT+TGG + chr5.2:76200969-76200988 None:intergenic 25.0%
!! GAAGTATTATCTCCAATATT+CGG + chr5.2:76200046-76200065 None:intergenic 25.0%
!! GTGAAGAATTCATTTTACAT+AGG - chr5.2:76201000-76201019 MS.gene016989:intron 25.0%
!!! TTTCCACATTGATAATGTTT+TGG - chr5.2:76198051-76198070 MS.gene016989:intron 25.0%
AACATACATCGTGAAAAACA+TGG + chr5.2:76198596-76198615 None:intergenic 30.0%
ACAAAAATCAATATAGGGCA+TGG + chr5.2:76198404-76198423 None:intergenic 30.0%
AGAAATTGACAAGAAGGATA+TGG - chr5.2:76200241-76200260 MS.gene016989:intron 30.0%
ATGACAGTTATGAATTGTAC+AGG - chr5.2:76200427-76200446 MS.gene016989:intron 30.0%
ATTATGCATGAGTAGTACAA+AGG + chr5.2:76199430-76199449 None:intergenic 30.0%
ATTATTTCATCTATGGGCAA+TGG + chr5.2:76198945-76198964 None:intergenic 30.0%
CACAAAATATCACATGTGTT+TGG + chr5.2:76198282-76198301 None:intergenic 30.0%
CCATAAAATTAACCTATGCA+AGG + chr5.2:76199674-76199693 None:intergenic 30.0%
GATACTTTACGACACATATA+TGG + chr5.2:76198442-76198461 None:intergenic 30.0%
GGTAACAGATTTGAACTATA+CGG + chr5.2:76200948-76200967 None:intergenic 30.0%
GTTCCAAAACATTATCAATG+TGG + chr5.2:76198057-76198076 None:intergenic 30.0%
GTTTGTTCACTGATACTTAT+TGG - chr5.2:76199216-76199235 MS.gene016989:intron 30.0%
TAAAATTAACCTATGCAAGG+AGG + chr5.2:76199671-76199690 None:intergenic 30.0%
TAAATGTGGAGTCATTTATC+AGG - chr5.2:76200011-76200030 MS.gene016989:intron 30.0%
TAAGCCTTCAATTGATATTG+TGG - chr5.2:76197963-76197982 MS.gene016989:intron 30.0%
TAGTCGAAGAGATGTTAAAA+CGG + chr5.2:76198888-76198907 None:intergenic 30.0%
TCACTTAATAATAACACGGT+CGG + chr5.2:76200886-76200905 None:intergenic 30.0%
TCTGTATCATACTATCTATC+TGG - chr5.2:76198460-76198479 MS.gene016989:intron 30.0%
TGATATCTCTGTGAATAACA+TGG + chr5.2:76198769-76198788 None:intergenic 30.0%
TTAACTTCAACTCACTATTC+TGG + chr5.2:76198083-76198102 None:intergenic 30.0%
! AGGCACATTTAAAATGTTCA+GGG - chr5.2:76201020-76201039 MS.gene016989:intron 30.0%
! ATTGTCATTTAACTCCACAT+AGG - chr5.2:76199118-76199137 MS.gene016989:intron 30.0%
! ATTTAGAATCGCAGCTATTT+AGG - chr5.2:76200576-76200595 MS.gene016989:intron 30.0%
! CCTTGCATAGGTTAATTTTA+TGG - chr5.2:76199671-76199690 MS.gene016989:intron 30.0%
! TAGGCACATTTAAAATGTTC+AGG - chr5.2:76201019-76201038 MS.gene016989:intron 30.0%
! TGAGTTGAAGTTAACATTGA+AGG - chr5.2:76198089-76198108 MS.gene016989:intron 30.0%
! TGGTGTGTTTTGTAAAATTG+CGG - chr5.2:76197983-76198002 MS.gene016989:intron 30.0%
! TTAGTACATTTTAAGCCTCA+CGG - chr5.2:76198718-76198737 MS.gene016989:intron 30.0%
AACATTGCAAGACAGTTGTT+TGG - chr5.2:76199049-76199068 MS.gene016989:intron 35.0%
AAGCTAAATGCACCATAAGA+TGG - chr5.2:76199625-76199644 MS.gene016989:intron 35.0%
AATATGTCAATGTTTCCGTG+AGG + chr5.2:76198736-76198755 None:intergenic 35.0%
ACTATAAAGCCTATGGAAGA+AGG + chr5.2:76199507-76199526 None:intergenic 35.0%
ACTATCTATCTGGACTTCTT+TGG - chr5.2:76198470-76198489 MS.gene016989:intron 35.0%
AGAACTTTGTGATCTGTATG+AGG + chr5.2:76198980-76198999 None:intergenic 35.0%
ATACCTTCGATACGTATTTC+TGG - chr5.2:76198183-76198202 MS.gene016989:intron 35.0%
ATAGCTGCGATTCTAAATAG+CGG + chr5.2:76200574-76200593 None:intergenic 35.0%
ATGCAAGGGTAAAATTCCAA+AGG + chr5.2:76199922-76199941 None:intergenic 35.0%
ATTCTAATCACCGATGAGAA+TGG - chr5.2:76201149-76201168 MS.gene016989:intron 35.0%
CAACAACTTGTACGTCAAAA+AGG + chr5.2:76199795-76199814 None:intergenic 35.0%
CACACCACAATATCAATTGA+AGG + chr5.2:76197970-76197989 None:intergenic 35.0%
CATAAAATGAGTAGTTGTGC+TGG + chr5.2:76201063-76201082 None:intergenic 35.0%
CTATAAAGCCTATGGAAGAA+GGG + chr5.2:76199506-76199525 None:intergenic 35.0%
CTCTGATTTCAATACCTATG+TGG + chr5.2:76199135-76199154 None:intergenic 35.0%
GAACGTACATCTTTGTAAGT+TGG + chr5.2:76201620-76201639 None:intergenic 35.0%
GAGTAACTGCTATATAAGAC+GGG - chr5.2:76200773-76200792 MS.gene016989:intron 35.0%
GATGAGATCATCAGCATAAT+TGG + chr5.2:76198864-76198883 None:intergenic 35.0%
GGAGTAACTGCTATATAAGA+CGG - chr5.2:76200772-76200791 MS.gene016989:intron 35.0%
TCCTAGAACTATAAAGCCTA+TGG + chr5.2:76199514-76199533 None:intergenic 35.0%
TGCATGAGAAATTGACAAGA+AGG - chr5.2:76200235-76200254 MS.gene016989:intron 35.0%
TGCATGGAAGGTTATCTTTA+AGG - chr5.2:76198119-76198138 MS.gene016989:intron 35.0%
TGGTTCATGAAACAACATAC+TGG - chr5.2:76199236-76199255 MS.gene016989:intron 35.0%
TTCAAGCTGAAGACTAAATG+TGG - chr5.2:76199997-76200016 MS.gene016989:intron 35.0%
TTTGACGTACAAGTTGTTGA+CGG - chr5.2:76199796-76199815 MS.gene016989:intron 35.0%
! ACATCTTTGTAAGTTGGAGA+TGG + chr5.2:76201614-76201633 None:intergenic 35.0%
! CAGCACAACTACTCATTTTA+TGG - chr5.2:76201061-76201080 MS.gene016989:intron 35.0%
! TCCATAGGCTTTATAGTTCT+AGG - chr5.2:76199510-76199529 MS.gene016989:intron 35.0%
! TGTGTTTTGTAAAATTGCGG+TGG - chr5.2:76197986-76198005 MS.gene016989:intron 35.0%
!! AAAAAGCAGCTCTGAAATCA+CGG + chr5.2:76200474-76200493 None:intergenic 35.0%
!! AAATTTGTTGCTTGATCACG+AGG - chr5.2:76198796-76198815 MS.gene016989:intron 35.0%
!! AGAAGTGCTTAAAAGCCAAA+CGG + chr5.2:76201470-76201489 None:intergenic 35.0%
!! AGACAGTTGGTTCTAAAAAG+CGG + chr5.2:76200640-76200659 None:intergenic 35.0%
AACTACATGATCATGCCGTT+TGG - chr5.2:76201452-76201471 MS.gene016989:intron 40.0%
ACAACTTACTTTGTTGCAGG+TGG - chr5.2:76197873-76197892 MS.gene016989:CDS 40.0%
ACTTGTACGTCAAAAAGGTC+TGG + chr5.2:76199790-76199809 None:intergenic 40.0%
ATAACACGGTCGGAAAATTG+AGG + chr5.2:76200876-76200895 None:intergenic 40.0%
ATTACAAGATCGAGGGTGAA+TGG - chr5.2:76199963-76199982 MS.gene016989:intron 40.0%
CAACTTACTTTGTTGCAGGT+GGG - chr5.2:76197874-76197893 MS.gene016989:CDS 40.0%
CAATATCGAGAGTTACCTCA+AGG - chr5.2:76201117-76201136 MS.gene016989:intron 40.0%
CATGCTAACACCATTCTCAT+CGG + chr5.2:76201162-76201181 None:intergenic 40.0%
CCAATAACATAAGCCATGCT+GGG + chr5.2:76200191-76200210 None:intergenic 40.0%
CTCCACATTGAAGAACACAA+CGG + chr5.2:76200756-76200775 None:intergenic 40.0%
GAAGTGTGTTTGAGTCTTTG+AGG + chr5.2:76199699-76199718 None:intergenic 40.0%
GTGGAGTCATTTATCAGGAT+TGG - chr5.2:76200016-76200035 MS.gene016989:intron 40.0%
GTTGAGTGAACTTTGTCTTG+AGG - chr5.2:76199582-76199601 MS.gene016989:intron 40.0%
TAGAATCTTCAGACTCCTTG+AGG + chr5.2:76201135-76201154 None:intergenic 40.0%
TCCAATAACATAAGCCATGC+TGG + chr5.2:76200192-76200211 None:intergenic 40.0%
TCCACATTGAAGAACACAAC+GGG + chr5.2:76200755-76200774 None:intergenic 40.0%
TGCACAACTTACTTTGTTGC+AGG - chr5.2:76197870-76197889 MS.gene016989:CDS 40.0%
TTGACGTACAAGTTGTTGAC+GGG - chr5.2:76199797-76199816 MS.gene016989:intron 40.0%
! AGGATTGGTGTACCGAATAT+TGG - chr5.2:76200031-76200050 MS.gene016989:intron 40.0%
AAAGCCTATGGAAGAAGGGA+GGG + chr5.2:76199502-76199521 None:intergenic 45.0%
AAAGTTCTCTCCCATGTCAC+CGG - chr5.2:76198992-76199011 MS.gene016989:intron 45.0%
AAGTAGCTTTCATGTCAGCG+TGG + chr5.2:76198016-76198035 None:intergenic 45.0%
ACCCGTTGTGTTCTTCAATG+TGG - chr5.2:76200751-76200770 MS.gene016989:intron 45.0%
ACGTGAACGGACATATGCAA+GGG + chr5.2:76199936-76199955 None:intergenic 45.0%
ATGCTGGGATCAGTGCATAA+GGG + chr5.2:76200176-76200195 None:intergenic 45.0%
ATGGAAGCAATGAGAAGTGG+GGG + chr5.2:76199744-76199763 None:intergenic 45.0%
ATGTTATTGTGAGCCATGGC+TGG + chr5.2:76199767-76199786 None:intergenic 45.0%
CAACATCACATGCACAACTG+CGG - chr5.2:76201404-76201423 MS.gene016989:intron 45.0%
CAGCCAGAAATACGTATCGA+AGG + chr5.2:76198189-76198208 None:intergenic 45.0%
CATTGAAGGCTGTAGTTGCA+TGG - chr5.2:76198103-76198122 MS.gene016989:intron 45.0%
CATTTCGGAGCTAGAACACA+AGG - chr5.2:76197813-76197832 MS.gene016989:CDS 45.0%
CCCAGCATGGCTTATGTTAT+TGG - chr5.2:76200188-76200207 MS.gene016989:intron 45.0%
CTGGATGTTATTGTGAGCCA+TGG + chr5.2:76199771-76199790 None:intergenic 45.0%
GAACCGCATTACAAGATCGA+GGG - chr5.2:76199956-76199975 MS.gene016989:intron 45.0%
GATGGAAGCAATGAGAAGTG+GGG + chr5.2:76199745-76199764 None:intergenic 45.0%
GGATGGAAGCAATGAGAAGT+GGG + chr5.2:76199746-76199765 None:intergenic 45.0%
TAAAGCCTATGGAAGAAGGG+AGG + chr5.2:76199503-76199522 None:intergenic 45.0%
TCACCCTCGATCTTGTAATG+CGG + chr5.2:76199962-76199981 None:intergenic 45.0%
TGAACCGCATTACAAGATCG+AGG - chr5.2:76199955-76199974 MS.gene016989:intron 45.0%
TGGATGGAAGCAATGAGAAG+TGG + chr5.2:76199747-76199766 None:intergenic 45.0%
TGTAATGCGGTTCACGTGAA+CGG + chr5.2:76199949-76199968 None:intergenic 45.0%
TTCTGTATCTGCAGCTCCTT+TGG - chr5.2:76199903-76199922 MS.gene016989:intron 45.0%
! CGATGAGAATGGTGTTAGCA+TGG - chr5.2:76201160-76201179 MS.gene016989:intron 45.0%
! TTATTCTGGTTGCAGTCGTG+CGG + chr5.2:76201574-76201593 None:intergenic 45.0%
ACACAAGGTTCAGACTCTGC+AGG - chr5.2:76197828-76197847 MS.gene016989:CDS 50.0%
ACTTCCCTCCCTTCTTCCAT+AGG - chr5.2:76199495-76199514 MS.gene016989:intron 50.0%
CACATGCACAACTGCGGATT+TGG - chr5.2:76201410-76201429 MS.gene016989:intron 50.0%
CACGTGAACGGACATATGCA+AGG + chr5.2:76199937-76199956 None:intergenic 50.0%
CATGCTGGGATCAGTGCATA+AGG + chr5.2:76200177-76200196 None:intergenic 50.0%
CGCAGTTGTGCATGTGATGT+TGG + chr5.2:76201406-76201425 None:intergenic 50.0%
GAAGGCTGTAGTTGCATGGA+AGG - chr5.2:76198107-76198126 MS.gene016989:intron 50.0%
GTGCATGTGATGTTGGTGTG+AGG + chr5.2:76201399-76201418 None:intergenic 50.0%
TATTGTGAGCCATGGCTGGA+TGG + chr5.2:76199763-76199782 None:intergenic 50.0%
TCATTGCTTCCATCCAGCCA+TGG - chr5.2:76199751-76199770 MS.gene016989:intron 50.0%
TGGAAGCAATGAGAAGTGGG+GGG + chr5.2:76199743-76199762 None:intergenic 50.0%
! CTTATGCACTGATCCCAGCA+TGG - chr5.2:76200175-76200194 MS.gene016989:intron 50.0%
! GTACAAGTTGTTGACGGGCA+TGG - chr5.2:76199802-76199821 MS.gene016989:intron 50.0%
! TATTCTGGTTGCAGTCGTGC+GGG + chr5.2:76201573-76201592 None:intergenic 50.0%
AGCAATGAGAAGTGGGGGGA+AGG + chr5.2:76199739-76199758 None:intergenic 55.0%
ATGTCGCAGCCTCCTTGCAT+AGG - chr5.2:76199659-76199678 MS.gene016989:intron 55.0%
TGCCGCACGGGTCATATTGA+CGG + chr5.2:76201260-76201279 None:intergenic 55.0%
TGCCGTCAATATGACCCGTG+CGG - chr5.2:76201255-76201274 MS.gene016989:intron 55.0%
AAGTGGGGGGAAGGCAGGAA+AGG + chr5.2:76199730-76199749 None:intergenic 60.0%
ACTTCAGCTCGCCGGTGACA+TGG + chr5.2:76199006-76199025 None:intergenic 60.0%
ATATGACCCGTGCGGCAGCA+CGG - chr5.2:76201263-76201282 MS.gene016989:intron 60.0%
ATGAGAAGTGGGGGGAAGGC+AGG + chr5.2:76199735-76199754 None:intergenic 60.0%
ATGTCACCGGCGAGCTGAAG+TGG - chr5.2:76199005-76199024 MS.gene016989:intron 60.0%
CTTCAGCTCGCCGGTGACAT+GGG + chr5.2:76199005-76199024 None:intergenic 60.0%
GAGCTTCCACTTCAGCTCGC+CGG + chr5.2:76199014-76199033 None:intergenic 60.0%
TATGACCCGTGCGGCAGCAC+GGG - chr5.2:76201264-76201283 MS.gene016989:intron 65.0%
ATGCACCCGTGCTGCCGCAC+GGG + chr5.2:76201272-76201291 None:intergenic 70.0%
CATGCACCCGTGCTGCCGCA+CGG + chr5.2:76201273-76201292 None:intergenic 70.0%
CGTGCGGCAGCACGGGTGCA+TGG - chr5.2:76201271-76201290 MS.gene016989:intron 75.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 76197805 76201654 76197805 ID=MS.gene016989
chr5.2 mRNA 76197805 76201654 76197805 ID=MS.gene016989.t1;Parent=MS.gene016989
chr5.2 exon 76201568 76201654 76201568 ID=MS.gene016989.t1.exon1;Parent=MS.gene016989.t1
chr5.2 CDS 76201568 76201654 76201568 ID=cds.MS.gene016989.t1;Parent=MS.gene016989.t1
chr5.2 exon 76200642 76200737 76200642 ID=MS.gene016989.t1.exon2;Parent=MS.gene016989.t1
chr5.2 CDS 76200642 76200737 76200642 ID=cds.MS.gene016989.t1;Parent=MS.gene016989.t1
chr5.2 exon 76197805 76197900 76197805 ID=MS.gene016989.t1.exon3;Parent=MS.gene016989.t1
chr5.2 CDS 76197805 76197900 76197805 ID=cds.MS.gene016989.t1;Parent=MS.gene016989.t1
Gene Sequence

>MS.gene016989

ATGCAATACATTTCGGAGCTAGAACACAAGGTTCAGACTCTGCAGGCTGAAGCAACTACACTTTCTGCACAACTTACTTTGTTGCAGGTGGGAACATTTATTTAAGTTTGTGAAACCGTGTTTTATTTTAGTAATTTGATCTTAGTTTCTTTTTTTACTAAGCCTTCAATTGATATTGTGGTGTGTTTTGTAAAATTGCGGTGGAAATCCACGCTGACATGAAAGCTACTTATTGTAGCTTCTTTGTTTCCACATTGATAATGTTTTGGAACTAACCAGAATAGTGAGTTGAAGTTAACATTGAAGGCTGTAGTTGCATGGAAGGTTATCTTTAAGGAACAACTTGAAAAGTACAAATTTCACCAATACTTCTTAGATATACCTTCGATACGTATTTCTGGCTGACGACACATTTGTCATCTAGATACGATACATCTTTGATACACATATTTCAAACTAAAGGCATATTTCTAACCAAACACATGTGATATTTTGTGATTTGTACACAAAAAGCATTCAGTATTTGTTTTAATATCTTTGATACGTATTTCTGACTCAAGACACGATTGTCTTAGTTTTTTAGTCAATGTATCGTGCCATGCCCTATATTGATTTTTGTAAGTTTCTCATATCACCATATATGTGTCGTAAAGTATCTGTATCATACTATCTATCTGGACTTCTTTGGAAGTATGATATGTATGTGATCATTTTAGCAGTTGAGCTAATTGTAATGACATGCTATGAAATATGGATTATAGAGAAAGTGAAAATTTGATTTGAAACTACCATGTTTTTCACGATGTATGTTATATTCTACCATTCTATGCTACTTATAATTTTACTCTTTCCTTTTGTAGCAATTCTTTGTATTATATTGAATCTTACTTAGTTTATTTCAACTTTGAAAATTTTAGTACATTTTAAGCCTCACGGAAACATTGACATATTGTCACGAGAACCATGTTATTCACAGAGATATCAAGCTTGAAAATTTGTTGCTTGATCACGAGGTATATATTTTTCTATTATTTTACTTATATTTGAACTGCTCTTCCAATTATGCTGATGATCTCATCGCCGTTTTAACATCTCTTCGACTACTAAATTTTGTTTTCTTTTTGATAGTGTTTCGATCCATTGCCCATAGATGAAATAATTGTTTTTAGTTGCCTCATACAGATCACAAAGTTCTCTCCCATGTCACCGGCGAGCTGAAGTGGAAGCTCTATGAGAGAAGACGAAACATTGCAAGACAGTTGTTTGGAGCTGTTTGTATAGTAACAATTTTTTTAAGATTTGACTTCTGTCTAATTGTCATTTAACTCCACATAGGTATTGAAATCAGAGTGAGAATAACACTCTTTTAGCTTTTATTTTGTCTATCAATTCTGTGTGCTTTTTTCTTAGTGTTTGTTCACTGATACTTATTGGTTCATGAAACAACATACTGGTTTGATAGCTATATTATTTGTAACTATCTGTTATTGAAAGTAGTTCACTAATAGGAATGCTTAAATTGATTTTGTTTCTAAACTTCAATTTGATTATAAGCGGTAAAAATAGTTTTTGCTTTGAATTGGAAAATTACATCAATATTTATATTTGGTGATTGGTGCTGTCCTTTGTACTACTCATGCATAATTATGCTGTACATATATCTCTGTTTTGATTGTACATTTTTGTTCAGACTTCCCTCCCTTCTTCCATAGGCTTTATAGTTCTAGGACAATTTATCAGATTAACATTAGATTTCTTATCATTGATCAAACTTTGGGTTGAGTGAACTTTGTCTTGAGGATTAATAAGTGCTACAAATGAAGCTAAATGCACCATAAGATGGTAAGATTATATATGTCGCAGCCTCCTTGCATAGGTTAATTTTATGGCACCTCAAAGACTCAAACACACTTCAGCTTAAACCTTTCCTGCCTTCCCCCCACTTCTCATTGCTTCCATCCAGCCATGGCTCACAATAACATCCAGACCTTTTTGACGTACAAGTTGTTGACGGGCATGGTTACTCTTATCTCTGATGTTGTGTTATGTTTCAGTTTGTTTTGATTCAAGACTATCTAACATATTGTTTTTATCATTTTTCTGTATCTGCAGCTCCTTTGGAATTTTACCCTTGCATATGTCCGTTCACGTGAACCGCATTACAAGATCGAGGGTGAATGGAAGCAATTTTGTTCAAGCTGAAGACTAAATGTGGAGTCATTTATCAGGATTGGTGTACCGAATATTGGAGATAATACTTCTTTCTTTATCACATTGAATTTTTAGATTATGCATTTGGTTGTCGAAGTGTATATATTCTGAGTGTTTATTGTCATTTTCATTCAGATGTGATAAATTATGTACTTTGCCCTTATGCACTGATCCCAGCATGGCTTATGTTATTGGACTATTCTACTTTTTAAATTGTACTGCATGAGAAATTGACAAGAAGGATATGGAGTTTCTGAATTCGCTTCATTTTCTTATTATGAAAATATGCTGCTCTATTTGAACTATATACAATGTAGCGTAAAATGATTTTATAATTGTTGATTTACAGTGACTTCAAAATGTGACAGTGTGATTTCAACCGTAATTATAATATGAGTTACTCACTAATAGATGACAGTTATGAATTGTACAGGTTATGATCAATTGTAGAAACACCGTGATTTCAGAGCTGCTTTTTACTGTGCTATTATTCAATCTAGAATCACCTCTATTTAGAACGATATTTTTGCAGCGAAATTGTTCGATTTATAGTCACCGCTATTTAGAATCGCAGCTATTTAGGACGAGATTTTTATTGTTTTGTTCTTCACTGTAAAATCACCGCTTTTTAGAACCAACTGTCTTAAATTTGTTATTTTTCTTCACTGTAAAATCAGAGATAAGATGAATACAGATTTTTAATTGTCATTGCACAACTATTTAGTAAAATGTTTTACCCGTTGTGTTCTTCAATGTGGAGTAACTGCTATATAAGACGGGATTTTTTGCACAGCTATTTAGTAAAATAAATTTTCCTGTTGTGTTGTTATTTTTCAATTGCATATCAACACTAAGTTCCTCAATTTTCCGACCGTGTTATTATTAAGTGAAAAATCACCGCTATTTAGAAACATATTTTCACTGTGTTACCGTATAGTTCAAATCTGTTACCAAGATTATAATCATCAAGCATATCATAAAGGTGTGAAGAATTCATTTTACATAGGCACATTTAAAATGTTCAGGGAAGCATAAAAAAATGCACCAGCACAACTACTCATTTTATGGCACGCTCAAGCATAAAAAATGCACTATTTTTCGCAATATCGAGAGTTACCTCAAGGAGTCTGAAGATTCTAATCACCGATGAGAATGGTGTTAGCATGGATTGTATTTTTACTATTAACATTGTATTTGCACCAAAAGCACATTATTTTCTATTTATTTGAATGTTCTATTTGCCGTCAATATGACCCGTGCGGCAGCACGGGTGCATGGTCTAGTTCCATATAATGATATAAAGCATTTATCAAACATAAAAACTATTTGCAATTTATAGGGTATAGACAAACATAAAATATTCATAGAAAATTTAAAGAGCCTCACACCAACATCACATGCACAACTGCGGATTTGGATTACTTGCAGTTGAAGTCAACTACATGATCATGCCGTTTGGCTTTTAAGCACTTCTCTCTTAATCTCTTTTTTTTTTTATATACAAACTTCATCTTATTATTTTTATAGTTTTACTTAAATGCAGGAATTCTCAATCCCGCACGACTGCAACCAGAATAAGTTTATGTTATGTTTTTCCATCTCCAACTTACAAAGATGTACGTTCAACGTGTCTTTCTAA
Protein sequence

>MS.gene016989.t1

MQYISELEHKVQTLQAEATTLSAQLTLLQYILSLTETLTYCHENHVIHRDIKLENLLLDHEEFSIPHDCNQNKFMLCFSISNLQRCTFNVSF