AlfalfaGEDB | MS.gene017323

M. sativa cultivar XinJiangDaYe / MS.gene017323

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017323.t1	NP_173829.3	56.2	73	31	1	7	79	13	84	4.00E-12	80.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017323.t1	O48686	56.2	73	31	1	7	79	13	84	9.7e-15	80.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017323.t1	A0A5B7BTU9	59.7	72	28	1	7	78	39	109	2.9e-12	80.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene017323.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017323.t1	MTR_1g105960	55.385	65	29	0	12	76	4	68	1.18e-18	73.9
MS.gene017323.t1	MTR_1g105955	53.846	65	30	0	12	76	98	162	1.38e-17	72.8
MS.gene017323.t1	MTR_1g105955	49.231	65	33	0	8	72	15	79	7.92e-15	65.9
MS.gene017323.t1	MTR_5g036670	51.351	74	35	1	7	80	42	114	1.41e-17	75.9
MS.gene017323.t1	MTR_6g008970	50.685	73	35	1	7	79	62	133	1.04e-16	73.2
MS.gene017323.t1	MTR_8g062700	51.351	74	35	1	7	80	41	113	1.81e-16	72.4
MS.gene017323.t1	MTR_8g062700	51.351	74	35	1	7	80	41	113	1.81e-16	72.4
MS.gene017323.t1	MTR_8g062350	47.887	71	36	1	6	76	12	81	8.53e-16	68.6
MS.gene017323.t1	MTR_1g109550	46.269	67	35	1	10	76	17	82	4.87e-15	65.5
MS.gene017323.t1	MTR_3g006065	52.239	67	32	0	8	74	90	156	1.54e-14	65.1
MS.gene017323.t1	MTR_3g006150	55.882	68	28	2	8	74	90	156	3.19e-14	63.9
MS.gene017323.t1	MTR_4g133300	52.239	67	32	0	12	78	175	241	3.28e-14	65.1
MS.gene017323.t1	MTR_4g133300	41.333	75	40	2	7	81	11	81	7.72e-12	58.9
MS.gene017323.t1	MTR_4g133470	52.239	67	32	0	12	78	175	241	3.91e-14	65.1
MS.gene017323.t1	MTR_4g133470	41.333	75	40	2	7	81	11	81	8.29e-12	58.5
MS.gene017323.t1	MTR_1g104940	47.761	67	35	0	12	78	17	83	7.70e-14	62.0
MS.gene017323.t1	MTR_1g093310	46.753	77	34	2	2	78	3	72	1.83e-13	59.7
MS.gene017323.t1	MTR_3g006185	51.515	66	31	1	12	76	101	166	3.03e-13	61.6
MS.gene017323.t1	MTR_1g091047	53.030	66	29	2	7	71	8	72	3.32e-13	58.9
MS.gene017323.t1	MTR_1g104960	40.789	76	45	0	7	82	97	172	1.25e-12	60.1
MS.gene017323.t1	MTR_1g104960	50.000	64	32	0	15	78	19	82	8.24e-12	57.8
MS.gene017323.t1	MTR_6g008995	45.833	72	38	1	8	79	37	107	1.32e-12	61.6
MS.gene017323.t1	MTR_2g097830	41.333	75	40	2	7	81	69	139	1.42e-12	59.7
MS.gene017323.t1	MTR_1g100260	49.315	73	33	2	13	81	28	100	2.90e-12	57.8
MS.gene017323.t1	MTR_3g006125	46.970	66	34	1	12	76	183	248	3.46e-12	59.7
MS.gene017323.t1	MTR_1g105010	43.478	69	39	0	12	80	19	87	5.26e-12	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017323.t1	AT1G24190	56.164	73	31	1	7	79	13	84	3.08e-19	80.5
MS.gene017323.t1	AT1G24190	56.164	73	31	1	7	79	13	84	3.24e-19	80.5
MS.gene017323.t1	AT1G24190	56.164	73	31	1	7	79	13	84	3.67e-19	80.1
MS.gene017323.t1	AT1G70060	54.167	72	32	1	7	78	13	83	1.68e-16	72.8
MS.gene017323.t1	AT1G70060	54.167	72	32	1	7	78	13	83	1.68e-16	72.8
MS.gene017323.t1	AT3G01320	51.389	72	34	1	7	78	56	126	3.24e-16	72.0
MS.gene017323.t1	AT3G01320	51.389	72	34	1	7	78	56	126	3.30e-16	72.0
MS.gene017323.t1	AT5G15020	50.685	73	35	1	7	79	51	122	4.21e-16	71.6
MS.gene017323.t1	AT5G15020	50.685	73	35	1	7	79	51	122	4.30e-16	71.6
MS.gene017323.t1	AT1G59890	50.000	72	35	1	8	79	45	115	7.89e-15	67.8
MS.gene017323.t1	AT1G59890	50.000	72	35	1	8	79	45	115	8.62e-15	67.8
MS.gene017323.t1	AT1G59890	50.000	72	35	1	8	79	45	115	8.87e-15	67.8
MS.gene017323.t1	AT1G59890	50.000	72	35	1	8	79	45	115	8.88e-15	67.8
MS.gene017323.t1	AT5G15025	49.315	73	36	1	7	79	46	117	1.11e-14	63.9
MS.gene017323.t1	AT1G70030	44.444	72	39	1	8	79	22	92	3.22e-14	63.2
MS.gene017323.t1	AT1G70030	44.444	72	39	1	8	79	22	92	8.51e-14	62.4
MS.gene017323.t1	AT1G70030	45.070	71	38	1	8	78	11	80	1.34e-13	62.0
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.08e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	241	309	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT4G12020	46.479	71	36	2	8	78	308	376	2.09e-12	61.2
MS.gene017323.t1	AT1G10450	46.479	71	37	1	8	78	84	153	3.44e-12	60.5
MS.gene017323.t1	AT1G10450	46.479	71	37	1	8	78	33	102	4.70e-12	60.1
MS.gene017323.t1	AT1G10450	46.479	71	37	1	8	78	33	102	4.70e-12	60.1
MS.gene017323.t1	AT1G24200	45.714	70	37	1	7	76	13	81	5.93e-12	58.5
MS.gene017323.t1	AT1G23810	39.706	68	41	0	3	70	103	170	2.50e-11	57.4
MS.gene017323.t1	AT5G15040	41.176	68	40	0	12	79	6	73	8.10e-11	53.1
MS.gene017323.t1	AT5G15040	41.176	68	40	0	12	79	6	73	8.10e-11	53.1
MS.gene017323.t1	AT5G15040	41.176	68	40	0	12	79	6	73	8.10e-11	53.1

Find 19 sgRNAs with CRISPR-Local

Find 28 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGGCATACTCACTTAATTT+TGG	0.125153	5.1:-59660115	MS.gene017323:CDS
CCAATGCATCGTCGCGGTTT+TGG	0.138304	5.1:+59660277	None:intergenic
AGAGTCAAGCAGCTGCTTAA+AGG	0.323406	5.1:-59660135	MS.gene017323:CDS
AGTATAATTTGATGTTCCTT+TGG	0.390609	5.1:+59660075	None:intergenic
GAGTCAAGCAGCTGCTTAAA+GGG	0.418662	5.1:-59660134	MS.gene017323:CDS
TAATTTGATGTTCCTTTGGC+AGG	0.455833	5.1:+59660079	None:intergenic
TGCATTGGCAGCGTTTCATA+AGG	0.456199	5.1:-59660262	MS.gene017323:CDS
CCAAAACCGCGACGATGCAT+TGG	0.481999	5.1:-59660277	MS.gene017323:CDS
TTGATGTTCCTTTGGCAGGA+AGG	0.538933	5.1:+59660083	None:intergenic
AAAACTCCTAAAAGATGTCA+AGG	0.541090	5.1:-59660190	MS.gene017323:CDS
CAAATTATACTTGAGACCAC+TGG	0.569052	5.1:-59660063	MS.gene017323:CDS
AGCTGCGTTTCAAGATCAGA+GGG	0.585156	5.1:-59660232	MS.gene017323:CDS
AAGCTGCGTTTCAAGATCAG+AGG	0.599823	5.1:-59660233	MS.gene017323:CDS
CAGCTGCTTGACTCTTGCCG+CGG	0.614341	5.1:+59660143	None:intergenic
ATTCAACACCTTCCTGCCAA+AGG	0.618562	5.1:-59660091	MS.gene017323:CDS
AATCATAAATTACTAACCAG+TGG	0.644366	5.1:+59660047	None:intergenic
AATTTACACAAGAGATGCCG+CGG	0.649048	5.1:-59660160	MS.gene017323:CDS
ACGCTGCCAATGCATCGTCG+CGG	0.755650	5.1:+59660271	None:intergenic
GCTGCGTTTCAAGATCAGAG+GGG	0.802684	5.1:-59660231	MS.gene017323:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AAAATAATTGATTTTAGTGA+AGG	+	chr5.1:59660078-59660097	None:intergenic	15.0%
!!!	ACTAAAATCAATTATTTTTC+TGG	-	chr5.1:59660080-59660099	MS.gene017323:CDS	15.0%
!!!	GTTTTATTATTCTGAATTAT+TGG	-	chr5.1:59660102-59660121	MS.gene017323:CDS	15.0%
!!!	TATACTGAATCATTAATTTT+CGG	-	chr5.1:59660132-59660151	MS.gene017323:CDS	15.0%
!	AGTATAATTTGATGTTCCTT+TGG	+	chr5.1:59660378-59660397	None:intergenic	25.0%
	AAAACTCCTAAAAGATGTCA+AGG	-	chr5.1:59660260-59660279	MS.gene017323:CDS	30.0%
!	TTTGATCCTTGACATCTTTT+AGG	+	chr5.1:59660269-59660288	None:intergenic	30.0%
	CAAATTATACTTGAGACCAC+TGG	-	chr5.1:59660387-59660406	MS.gene017323:intron	35.0%
	TAATTTGATGTTCCTTTGGC+AGG	+	chr5.1:59660374-59660393	None:intergenic	35.0%
!	AGGGCATACTCACTTAATTT+TGG	-	chr5.1:59660335-59660354	MS.gene017323:intron	35.0%
!	GGGCATACTCACTTAATTTT+GGG	-	chr5.1:59660336-59660355	MS.gene017323:intron	35.0%
!!	ATTGATTTTAGTGAAGGACG+AGG	+	chr5.1:59660072-59660091	None:intergenic	35.0%
	AATTTACACAAGAGATGCCG+CGG	-	chr5.1:59660290-59660309	MS.gene017323:intron	40.0%
	AAGCTGCGTTTCAAGATCAG+AGG	-	chr5.1:59660217-59660236	MS.gene017323:CDS	45.0%
	AGAGTCAAGCAGCTGCTTAA+AGG	-	chr5.1:59660315-59660334	MS.gene017323:intron	45.0%
	AGCTGCGTTTCAAGATCAGA+GGG	-	chr5.1:59660218-59660237	MS.gene017323:CDS	45.0%
	ATTCAACACCTTCCTGCCAA+AGG	-	chr5.1:59660359-59660378	MS.gene017323:intron	45.0%
	GAGTCAAGCAGCTGCTTAAA+GGG	-	chr5.1:59660316-59660335	MS.gene017323:intron	45.0%
	TTGATGTTCCTTTGGCAGGA+AGG	+	chr5.1:59660370-59660389	None:intergenic	45.0%
!	TGCATTGGCAGCGTTTCATA+AGG	-	chr5.1:59660188-59660207	MS.gene017323:CDS	45.0%
	GCTGCGTTTCAAGATCAGAG+GGG	-	chr5.1:59660219-59660238	MS.gene017323:CDS	50.0%
!	AATGCATCGTCGCGGTTTTG+GGG	+	chr5.1:59660174-59660193	None:intergenic	50.0%
!	CAATGCATCGTCGCGGTTTT+GGG	+	chr5.1:59660175-59660194	None:intergenic	50.0%
	CCAAAACCGCGACGATGCAT+TGG	-	chr5.1:59660173-59660192	MS.gene017323:CDS	55.0%
!	CCAATGCATCGTCGCGGTTT+TGG	+	chr5.1:59660176-59660195	None:intergenic	55.0%
!!	ATGCATCGTCGCGGTTTTGG+GGG	+	chr5.1:59660173-59660192	None:intergenic	55.0%
	ACGCTGCCAATGCATCGTCG+CGG	+	chr5.1:59660182-59660201	None:intergenic	60.0%
	CAGCTGCTTGACTCTTGCCG+CGG	+	chr5.1:59660310-59660329	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	59660059	59660413	59660059	ID=MS.gene017323
chr5.1	mRNA	59660059	59660413	59660059	ID=MS.gene017323.t1;Parent=MS.gene017323
chr5.1	exon	59660411	59660413	59660411	ID=MS.gene017323.t1.exon1;Parent=MS.gene017323.t1
chr5.1	CDS	59660411	59660413	59660411	ID=cds.MS.gene017323.t1;Parent=MS.gene017323.t1
chr5.1	exon	59660059	59660304	59660059	ID=MS.gene017323.t1.exon2;Parent=MS.gene017323.t1
chr5.1	CDS	59660059	59660304	59660059	ID=cds.MS.gene017323.t1;Parent=MS.gene017323.t1

Gene Sequence	>MS.gene017323 ATGGTATGCTCCTCGTCCTTCACTAAAATCAATTATTTTTCTGGTTTTATTATTCTGAATTATTGGAATTAGTTATACTGAATCATTAATTTTCGGAATAATGATGAAGATCCCCCAAAACCGCGACGATGCATTGGCAGCGTTTCATAAGGCTGTACAAGCTGCGTTTCAAGATCAGAGGGGAGATTACAATGAGCTTTTAAAACTCCTAAAAGATGTCAAGGATCAAAGAATTTACACAAGAGATGCCGCGGCAAGAGTCAAGCAGCTGCTTAAAGGGCATACTCACTTAATTTTGGGATTCAACACCTTCCTGCCAAAGGAACATCAAATTATACTTGAGACCACTGGTTAG
Protein sequence	>MS.gene017323.t1 MIPQNRDDALAAFHKAVQAAFQDQRGDYNELLKLLKDVKDQRIYTRDAAARVKQLLKGHTHLILGFNTFLPKEHQIILETTG