Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017520.t1 | KEH20354.1 | 98.2 | 109 | 0 | 1 | 1 | 107 | 1 | 109 | 2.00E-49 | 204.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017520.t1 | P40619 | 62.9 | 97 | 34 | 1 | 11 | 105 | 10 | 106 | 9.9e-26 | 117.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017520.t1 | A0A072TSA9 | 98.2 | 109 | 0 | 1 | 1 | 107 | 1 | 109 | 1.4e-49 | 204.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene017520.t1 | TR | HMG |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017520.t1 | MTR_8g073345 | 98.165 | 109 | 0 | 1 | 1 | 107 | 1 | 109 | 7.83e-73 | 213 |
| MS.gene017520.t1 | MTR_8g073345 | 98.165 | 109 | 0 | 1 | 1 | 107 | 1 | 109 | 2.27e-72 | 211 |
| MS.gene017520.t1 | MTR_5g024180 | 66.364 | 110 | 34 | 2 | 1 | 108 | 1 | 109 | 5.51e-45 | 142 |
| MS.gene017520.t1 | MTR_3g115580 | 63.393 | 112 | 33 | 4 | 3 | 106 | 5 | 116 | 2.74e-35 | 118 |
| MS.gene017520.t1 | MTR_7g082820 | 60.976 | 82 | 31 | 1 | 28 | 108 | 4 | 85 | 7.63e-28 | 99.4 |
| MS.gene017520.t1 | MTR_7g068280 | 63.158 | 76 | 26 | 1 | 33 | 106 | 47 | 122 | 6.84e-27 | 97.8 |
| MS.gene017520.t1 | MTR_4g094622 | 48.052 | 77 | 38 | 1 | 33 | 107 | 60 | 136 | 8.30e-21 | 81.6 |
| MS.gene017520.t1 | MTR_1g115220 | 50.000 | 74 | 35 | 1 | 33 | 104 | 98 | 171 | 4.89e-20 | 81.3 |
| MS.gene017520.t1 | MTR_5g024180 | 56.061 | 66 | 24 | 2 | 44 | 104 | 3 | 68 | 1.45e-16 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017520.t1 | AT1G20696 | 68.539 | 89 | 26 | 1 | 25 | 111 | 23 | 111 | 1.00e-34 | 116 |
| MS.gene017520.t1 | AT1G20696 | 70.930 | 86 | 23 | 1 | 25 | 108 | 23 | 108 | 2.16e-34 | 116 |
| MS.gene017520.t1 | AT1G20693 | 67.391 | 92 | 24 | 2 | 18 | 107 | 23 | 110 | 5.16e-34 | 115 |
| MS.gene017520.t1 | AT1G20693 | 67.391 | 92 | 24 | 2 | 18 | 107 | 23 | 110 | 6.82e-34 | 114 |
| MS.gene017520.t1 | AT1G20693 | 67.391 | 92 | 24 | 2 | 18 | 107 | 23 | 110 | 7.02e-34 | 114 |
| MS.gene017520.t1 | AT1G20696 | 70.588 | 85 | 23 | 1 | 25 | 107 | 23 | 107 | 3.20e-33 | 113 |
| MS.gene017520.t1 | AT2G17560 | 55.238 | 105 | 41 | 2 | 5 | 107 | 7 | 107 | 1.63e-31 | 108 |
| MS.gene017520.t1 | AT2G17560 | 55.238 | 105 | 41 | 2 | 5 | 107 | 7 | 107 | 1.63e-31 | 108 |
| MS.gene017520.t1 | AT2G17560 | 55.238 | 105 | 41 | 2 | 5 | 107 | 7 | 107 | 1.91e-31 | 108 |
| MS.gene017520.t1 | AT4G35570 | 46.789 | 109 | 51 | 2 | 3 | 109 | 5 | 108 | 2.03e-30 | 105 |
| MS.gene017520.t1 | AT4G35570 | 46.789 | 109 | 51 | 2 | 3 | 109 | 5 | 108 | 2.03e-30 | 105 |
| MS.gene017520.t1 | AT3G51880 | 62.353 | 85 | 30 | 1 | 25 | 107 | 41 | 125 | 7.63e-29 | 102 |
| MS.gene017520.t1 | AT3G51880 | 62.353 | 85 | 30 | 1 | 25 | 107 | 41 | 125 | 9.48e-29 | 102 |
| MS.gene017520.t1 | AT3G51880 | 62.353 | 85 | 30 | 1 | 25 | 107 | 41 | 125 | 9.48e-29 | 102 |
| MS.gene017520.t1 | AT3G51880 | 62.353 | 85 | 30 | 1 | 25 | 107 | 41 | 125 | 9.48e-29 | 102 |
| MS.gene017520.t1 | AT3G51880 | 62.353 | 85 | 30 | 1 | 25 | 107 | 41 | 125 | 1.07e-28 | 102 |
| MS.gene017520.t1 | AT1G20696 | 61.538 | 65 | 23 | 1 | 45 | 107 | 1 | 65 | 7.18e-19 | 75.5 |
| MS.gene017520.t1 | AT1G20696 | 61.538 | 65 | 23 | 1 | 45 | 107 | 1 | 65 | 9.88e-19 | 75.1 |
| MS.gene017520.t1 | AT2G34450 | 43.836 | 73 | 39 | 1 | 37 | 107 | 63 | 135 | 1.26e-17 | 73.6 |
| MS.gene017520.t1 | AT2G34450 | 43.836 | 73 | 39 | 1 | 37 | 107 | 63 | 135 | 1.26e-17 | 73.6 |
| MS.gene017520.t1 | AT2G34450 | 43.836 | 73 | 39 | 1 | 37 | 107 | 63 | 135 | 1.26e-17 | 73.6 |
| MS.gene017520.t1 | AT2G34450 | 43.243 | 74 | 39 | 2 | 37 | 107 | 63 | 136 | 4.09e-16 | 69.7 |
| MS.gene017520.t1 | AT5G23420 | 48.611 | 72 | 32 | 3 | 28 | 96 | 106 | 175 | 9.09e-13 | 62.4 |
Find 0 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TAATTTTTCGAAATTTAATT+AGG | - | 57641:47734-47753 | MS.gene017520:intron | 10.0% |
| !!! | TTAATTTTAATTGATGATTT+AGG | - | 57641:47557-47576 | MS.gene017520:intron | 10.0% |
| !! | CTAAAATCAACAAAAAAAGA+TGG | + | 57641:47354-47373 | None:intergenic | 20.0% |
| !! | CTAATCAGATCTAAAAAAAT+TGG | + | 57641:47285-47304 | None:intergenic | 20.0% |
| !!! | ATTTTTTGGATCATAATTTG+TGG | - | 57641:47677-47696 | MS.gene017520:intron | 20.0% |
| !!! | TTTTCGAAATTTAATTAGGT+CGG | - | 57641:47738-47757 | MS.gene017520:intron | 20.0% |
| !! | AATTTGTGGATTTTTACTGT+CGG | - | 57641:47691-47710 | MS.gene017520:intron | 25.0% |
| !!! | CTGATTTGTTCGTTTTTTAA+CGG | - | 57641:47529-47548 | MS.gene017520:intron | 25.0% |
| !!! | CTTATACTGTTCTCTAAAAT+CGG | + | 57641:47584-47603 | None:intergenic | 25.0% |
| CAACCAAAACAGATTCAAAT+CGG | + | 57641:47495-47514 | None:intergenic | 30.0% | |
| TTGGAGAAGAAAAAATCGAA+CGG | + | 57641:47266-47285 | None:intergenic | 30.0% | |
| ! | TTGGAATCAGCTTTAACTTT+CGG | + | 57641:47213-47232 | None:intergenic | 30.0% |
| !!! | GTACTGTGATTTTGTTTTTC+AGG | - | 57641:47898-47917 | MS.gene017520:intron | 30.0% |
| !!! | TGATTTTGTTTTTCAGGACA+AGG | - | 57641:47904-47923 | MS.gene017520:intron | 30.0% |
| GTAAACGAGATTTCTTACAG+CGG | + | 57641:47635-47654 | None:intergenic | 35.0% | |
| ! | ATTCACCAGTTTCTTGTTGT+AGG | + | 57641:47987-48006 | None:intergenic | 35.0% |
| !! | ATGAAAACGAAGAAAGCACT+TGG | + | 57641:47456-47475 | None:intergenic | 35.0% |
| !! | TCTCCGATTTGAATCTGTTT+TGG | - | 57641:47489-47508 | MS.gene017520:intron | 35.0% |
| AATTAGGTCGGAAAAGCTTG+CGG | - | 57641:47750-47769 | MS.gene017520:intron | 40.0% | |
| ACAAGCCTACAACAAGAAAC+TGG | - | 57641:47979-47998 | MS.gene017520:intron | 40.0% | |
| TTCTTTCTTCTTCAATGCCC+TGG | + | 57641:47942-47961 | None:intergenic | 40.0% | |
| !! | AAAACGAAGAAAGCACTTGG+AGG | + | 57641:47453-47472 | None:intergenic | 40.0% |
| !!! | GATTTTAGCTTGAAGCGCAA+AGG | - | 57641:47366-47385 | MS.gene017520:intron | 40.0% |
| AACGGTGAGAAGCGATTTAC+CGG | + | 57641:47248-47267 | None:intergenic | 45.0% | |
| ATTCCAAACCCGCTGATAAC+CGG | - | 57641:47226-47245 | MS.gene017520:CDS | 45.0% | |
| GAGATTTCTTACAGCGGCAA+CGG | + | 57641:47629-47648 | None:intergenic | 45.0% | |
| ! | AAAGCACTTGGAGGTCTCTT+TGG | + | 57641:47444-47463 | None:intergenic | 45.0% |
| ! | AGCGATTTACCGGTTATCAG+CGG | + | 57641:47238-47257 | None:intergenic | 45.0% |
| !!! | TTTTTAGTTATTTTATTTTT+TGG | - | 57641:47663-47682 | MS.gene017520:intron | 5.0% |
| ACAGACGAAGAAAGCAGCGA+AGG | - | 57641:47407-47426 | MS.gene017520:intron | 50.0% | |
| GAAAAGCTTGCGGTGAAGCA+TGG | - | 57641:47760-47779 | MS.gene017520:CDS | 50.0% | |
| TTCAATGCCCTGGCAGCATA+AGG | + | 57641:47932-47951 | None:intergenic | 50.0% | |
| ! | GCGATTTACCGGTTATCAGC+GGG | + | 57641:47237-47256 | None:intergenic | 50.0% |
| ! | TTACCGGTTATCAGCGGGTT+TGG | + | 57641:47232-47251 | None:intergenic | 50.0% |
| !! | GCGGCAACGGATTTGTTGTT+AGG | + | 57641:47616-47635 | None:intergenic | 50.0% |
| !! | GGAGGTCTCTTTGGCTTGTT+AGG | + | 57641:47435-47454 | None:intergenic | 50.0% |
| ACAAGGCTCCTTATGCTGCC+AGG | - | 57641:47921-47940 | MS.gene017520:intron | 55.0% | |
| CAAGGCTCCTTATGCTGCCA+GGG | - | 57641:47922-47941 | MS.gene017520:intron | 55.0% | |
| ! | AGCTTGAAGCGCAAAGGTGC+TGG | - | 57641:47372-47391 | MS.gene017520:intron | 55.0% |
| !! | AAGCGCAAAGGTGCTGGAGC+TGG | - | 57641:47378-47397 | MS.gene017520:intron | 60.0% |
| !! | GCAAAGGTGCTGGAGCTGGA+AGG | - | 57641:47382-47401 | MS.gene017520:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57641 | gene | 47207 | 48021 | 47207 | ID=MS.gene017520 |
| 57641 | mRNA | 47207 | 48021 | 47207 | ID=MS.gene017520.t1;Parent=MS.gene017520 |
| 57641 | exon | 47981 | 48021 | 47981 | ID=MS.gene017520.t1.exon1;Parent=MS.gene017520.t1 |
| 57641 | CDS | 47981 | 48021 | 47981 | ID=cds.MS.gene017520.t1;Parent=MS.gene017520.t1 |
| 57641 | exon | 47753 | 47854 | 47753 | ID=MS.gene017520.t1.exon2;Parent=MS.gene017520.t1 |
| 57641 | CDS | 47753 | 47854 | 47753 | ID=cds.MS.gene017520.t1;Parent=MS.gene017520.t1 |
| 57641 | exon | 47592 | 47649 | 47592 | ID=MS.gene017520.t1.exon3;Parent=MS.gene017520.t1 |
| 57641 | CDS | 47592 | 47649 | 47592 | ID=cds.MS.gene017520.t1;Parent=MS.gene017520.t1 |
| 57641 | exon | 47428 | 47472 | 47428 | ID=MS.gene017520.t1.exon4;Parent=MS.gene017520.t1 |
| 57641 | CDS | 47428 | 47472 | 47428 | ID=cds.MS.gene017520.t1;Parent=MS.gene017520.t1 |
| 57641 | exon | 47207 | 47302 | 47207 | ID=MS.gene017520.t1.exon5;Parent=MS.gene017520.t1 |
| 57641 | CDS | 47207 | 47302 | 47207 | ID=cds.MS.gene017520.t1;Parent=MS.gene017520.t1 |
| Gene Sequence |
| Protein sequence |
