AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene017688

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017688.t1 MTR_7g089010 95.724 304 5 4 1 301 1 299 0.0 575
MS.gene017688.t1 MTR_4g064730 44.292 219 65 4 5 182 8 210 4.18e-44 152
MS.gene017688.t1 MTR_4g121020 67.647 68 22 0 86 153 106 173 3.24e-25 105
MS.gene017688.t1 MTR_4g121020 67.647 68 22 0 86 153 210 277 3.52e-25 105
MS.gene017688.t1 MTR_4g121020 67.647 68 22 0 86 153 210 277 3.52e-25 105
MS.gene017688.t1 MTR_2g450070 58.442 77 32 0 90 166 212 288 1.39e-22 98.2
MS.gene017688.t1 MTR_2g450070 58.442 77 32 0 90 166 212 288 1.46e-22 98.2
MS.gene017688.t1 MTR_2g034960 60.294 68 27 0 86 153 206 273 5.43e-22 96.3
MS.gene017688.t1 MTR_3g106220 54.545 77 34 1 78 154 186 261 6.40e-22 96.3
MS.gene017688.t1 MTR_7g117705 63.636 66 21 1 90 155 190 252 3.54e-21 93.6
MS.gene017688.t1 MTR_1g032570 60.938 64 25 0 90 153 130 193 7.77e-21 92.8
MS.gene017688.t1 MTR_1g032570 60.938 64 25 0 90 153 199 262 9.25e-21 92.8
MS.gene017688.t1 MTR_1g013170 65.000 60 21 0 90 149 190 249 3.97e-20 90.5
MS.gene017688.t1 MTR_7g026400 34.559 136 75 3 82 217 148 269 5.42e-20 90.5
MS.gene017688.t1 MTR_4g131570 65.574 61 21 0 88 148 200 260 6.25e-20 90.1
MS.gene017688.t1 MTR_4g131600 54.412 68 31 0 82 149 192 259 2.28e-19 88.6
MS.gene017688.t1 MTR_0450s0040 52.857 70 33 0 88 157 166 235 4.45e-19 86.7
MS.gene017688.t1 MTR_1g013180 54.930 71 32 0 90 160 341 411 4.79e-19 87.8
MS.gene017688.t1 MTR_4g131580 65.574 61 21 0 88 148 197 257 4.87e-19 87.8
MS.gene017688.t1 MTR_3g102600 54.545 66 28 1 90 155 82 145 4.34e-18 80.5
MS.gene017688.t1 MTR_3g102600 54.545 66 28 1 90 155 131 194 7.18e-18 81.3
MS.gene017688.t1 MTR_8g079940 56.164 73 28 1 89 157 207 279 2.61e-17 82.4
MS.gene017688.t1 MTR_3g086100 51.389 72 35 0 90 161 127 198 7.05e-17 80.9
MS.gene017688.t1 MTR_3g086100 51.389 72 35 0 90 161 198 269 9.12e-17 80.9
MS.gene017688.t1 MTR_5g014040 56.667 60 26 0 90 149 310 369 1.63e-16 80.1
MS.gene017688.t1 MTR_4g098870 55.000 60 27 0 90 149 309 368 7.26e-16 78.2
MS.gene017688.t1 MTR_4g098870 58.929 56 23 0 90 145 309 364 7.57e-16 78.2
MS.gene017688.t1 MTR_4g098870 58.929 56 23 0 90 145 329 384 8.17e-16 78.2
MS.gene017688.t1 MTR_4g098870 55.000 60 27 0 90 149 329 388 8.36e-16 77.8
MS.gene017688.t1 MTR_4g098870 55.000 60 27 0 90 149 348 407 9.00e-16 77.8
MS.gene017688.t1 MTR_4g086835 51.515 66 32 0 84 149 267 332 1.05e-15 77.4
MS.gene017688.t1 MTR_4g021760 56.897 58 25 0 90 147 168 225 1.79e-15 75.9
MS.gene017688.t1 MTR_4g078810 51.667 60 29 0 90 149 157 216 2.24e-15 76.3
MS.gene017688.t1 MTR_2g090675 33.566 143 59 4 90 219 160 279 2.31e-15 76.3
MS.gene017688.t1 MTR_3g102590 44.304 79 42 1 90 166 142 220 2.90e-15 74.3
MS.gene017688.t1 MTR_5g014040 52.308 65 26 1 90 149 270 334 1.67e-14 73.9
MS.gene017688.t1 MTR_4g086835 50.746 67 32 1 84 149 267 333 1.80e-14 73.6
MS.gene017688.t1 MTR_5g014040 52.308 65 26 1 90 149 310 374 1.93e-14 73.9
MS.gene017688.t1 MTR_5g017980 52.542 59 28 0 90 148 191 249 4.64e-14 72.0
MS.gene017688.t1 MTR_5g017980 52.542 59 28 0 90 148 192 250 4.77e-14 71.6
MS.gene017688.t1 MTR_4g021790 54.237 59 27 0 90 148 167 225 6.86e-14 70.9
MS.gene017688.t1 MTR_4g113140 48.571 70 35 1 80 148 224 293 7.95e-14 71.6
MS.gene017688.t1 MTR_8g105600 57.895 57 24 0 94 150 190 246 1.12e-13 71.6
MS.gene017688.t1 MTR_5g041350 56.364 55 23 1 91 144 15 69 1.76e-13 70.5
MS.gene017688.t1 MTR_2g086450 55.385 65 27 2 91 154 45 108 4.23e-13 69.3
MS.gene017688.t1 MTR_5g017980 51.667 60 28 1 90 148 192 251 1.20e-12 67.8
MS.gene017688.t1 MTR_6g032990 40.000 95 45 3 91 181 42 128 1.20e-12 68.2
MS.gene017688.t1 MTR_6g444980 60.000 55 21 1 91 144 35 89 1.41e-12 67.8
MS.gene017688.t1 MTR_6g444980 60.000 55 21 1 91 144 35 89 1.49e-12 67.8
MS.gene017688.t1 MTR_1g090670 46.429 84 41 2 81 160 31 114 1.53e-12 67.0
MS.gene017688.t1 MTR_2g027800 50.685 73 35 1 91 162 39 111 1.71e-12 67.0
MS.gene017688.t1 MTR_4g021855 52.542 59 28 0 90 148 167 225 1.88e-12 67.0
MS.gene017688.t1 MTR_1g093080 50.000 60 29 1 90 148 210 269 1.96e-12 67.4
MS.gene017688.t1 MTR_7g098250 50.725 69 31 2 91 158 262 328 2.16e-12 67.8
MS.gene017688.t1 MTR_5g054300 51.667 60 28 1 90 148 197 256 3.00e-12 66.6
MS.gene017688.t1 MTR_1g053830 44.928 69 37 1 77 144 4 72 3.21e-12 62.4
MS.gene017688.t1 MTR_5g027440 56.364 55 23 1 91 144 25 79 3.79e-12 65.9
MS.gene017688.t1 MTR_3g102600 47.170 53 28 0 90 142 82 134 4.73e-12 62.8
MS.gene017688.t1 MTR_2g027800 60.000 55 21 1 91 144 39 93 5.50e-12 65.5
MS.gene017688.t1 MTR_3g102600 47.170 53 28 0 90 142 131 183 7.13e-12 63.5
MS.gene017688.t1 MTR_1g080330 55.556 54 23 1 91 143 175 228 9.30e-12 65.5
MS.gene017688.t1 MTR_1g080330 55.556 54 23 1 91 143 175 228 1.00e-11 64.7
MS.gene017688.t1 MTR_6g045327 48.276 58 30 0 90 147 171 228 1.07e-11 65.1
MS.gene017688.t1 MTR_4g081710 52.727 55 25 1 91 144 17 71 1.68e-11 64.3
MS.gene017688.t1 MTR_2g027860 58.182 55 22 1 91 144 49 103 1.84e-11 64.3
MS.gene017688.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 2.19e-11 63.9
MS.gene017688.t1 MTR_8g066310 41.905 105 54 4 43 143 125 226 2.74e-11 63.9
MS.gene017688.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 2.82e-11 63.5
MS.gene017688.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 3.50e-11 63.9
MS.gene017688.t1 MTR_7g088070 42.857 105 46 4 78 170 37 139 3.57e-11 62.8
MS.gene017688.t1 MTR_7g115530 53.704 54 24 1 91 143 218 271 3.84e-11 63.5
MS.gene017688.t1 MTR_0223s0040 56.364 55 23 1 90 143 17 71 8.66e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017688.t1 AT3G10760 54.571 350 95 10 1 301 1 335 2.26e-91 275
MS.gene017688.t1 AT5G05090 54.369 309 90 10 1 301 1 266 1.87e-80 244
MS.gene017688.t1 AT2G40970 61.682 214 57 6 1 199 1 204 1.23e-65 206
MS.gene017688.t1 AT3G46640 37.736 318 132 7 5 270 15 318 1.95e-52 175
MS.gene017688.t1 AT3G46640 37.736 318 132 7 5 270 15 318 1.95e-52 175
MS.gene017688.t1 AT3G46640 38.558 319 113 8 5 278 15 295 2.46e-52 174
MS.gene017688.t1 AT5G59570 45.933 209 66 4 11 184 28 224 4.65e-45 155
MS.gene017688.t1 AT5G59570 45.933 209 66 4 11 184 28 224 4.65e-45 155
MS.gene017688.t1 AT3G16857 60.000 75 30 0 79 153 155 229 4.38e-24 102
MS.gene017688.t1 AT3G16857 60.000 75 30 0 79 153 225 299 5.24e-24 102
MS.gene017688.t1 AT3G16857 60.000 75 30 0 79 153 225 299 5.87e-24 102
MS.gene017688.t1 AT5G58080 66.129 62 21 0 88 149 191 252 5.61e-22 96.3
MS.gene017688.t1 AT2G01760 63.077 65 24 0 90 154 134 198 2.96e-21 92.0
MS.gene017688.t1 AT2G01760 63.077 65 24 0 90 154 199 263 7.43e-21 92.0
MS.gene017688.t1 AT2G25180 61.538 65 25 0 90 154 194 258 9.56e-21 92.8
MS.gene017688.t1 AT2G01760 63.077 65 24 0 90 154 227 291 1.21e-20 91.7
MS.gene017688.t1 AT4G31920 61.667 60 23 0 90 149 182 241 4.00e-19 87.8
MS.gene017688.t1 AT5G07210 50.667 75 35 1 82 154 221 295 3.44e-17 82.4
MS.gene017688.t1 AT5G07210 50.667 75 35 1 82 154 213 287 3.54e-17 82.0
MS.gene017688.t1 AT3G62670 38.710 124 66 4 90 205 210 331 4.80e-16 78.6
MS.gene017688.t1 AT5G44190 55.000 60 27 0 90 149 146 205 5.42e-16 78.2
MS.gene017688.t1 AT5G44190 55.000 60 27 0 90 149 146 205 6.33e-16 77.4
MS.gene017688.t1 AT2G20570 55.000 60 27 0 90 149 152 211 3.88e-15 75.9
MS.gene017688.t1 AT2G20570 57.895 57 24 0 93 149 171 227 9.08e-15 74.7
MS.gene017688.t1 AT1G67710 59.322 59 23 1 93 150 121 179 1.98e-14 73.6
MS.gene017688.t1 AT1G67710 59.322 59 23 1 93 150 130 188 2.16e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT4G18020 54.237 59 27 0 91 149 296 354 2.44e-14 73.6
MS.gene017688.t1 AT1G67710 59.322 59 23 1 93 150 195 253 2.99e-14 73.2
MS.gene017688.t1 AT3G12730 61.818 55 20 1 91 144 23 77 3.03e-14 71.2
MS.gene017688.t1 AT2G27070 48.529 68 35 0 82 149 216 283 3.70e-14 73.2
MS.gene017688.t1 AT3G04030 57.407 54 23 0 91 144 45 98 9.75e-14 71.2
MS.gene017688.t1 AT5G45580 58.182 55 22 1 91 144 7 61 2.40e-13 69.3
MS.gene017688.t1 AT1G79430 61.818 55 20 1 91 144 34 88 2.61e-13 70.1
MS.gene017688.t1 AT5G45580 58.182 55 22 1 91 144 23 77 2.78e-13 69.3
MS.gene017688.t1 AT2G03500 53.333 60 27 1 90 148 232 291 3.36e-13 70.1
MS.gene017688.t1 AT3G04030 58.182 55 22 1 91 144 45 99 4.80e-13 69.3
MS.gene017688.t1 AT3G04030 58.182 55 22 1 91 144 45 99 5.32e-13 69.3
MS.gene017688.t1 AT3G24120 60.000 55 21 1 91 144 41 95 5.99e-13 68.6
MS.gene017688.t1 AT3G24120 60.000 55 21 1 91 144 41 95 6.12e-13 68.2
MS.gene017688.t1 AT4G13640 60.000 55 21 1 91 144 37 91 6.80e-13 68.2
MS.gene017688.t1 AT4G13640 60.000 55 21 1 91 144 37 91 7.15e-13 68.2
MS.gene017688.t1 AT5G18240 60.000 55 21 1 91 144 45 99 9.35e-13 68.6
MS.gene017688.t1 AT5G18240 60.000 55 21 1 91 144 45 99 9.35e-13 68.6
MS.gene017688.t1 AT5G49240 52.727 55 26 0 86 140 218 272 9.79e-13 67.8
MS.gene017688.t1 AT5G18240 60.000 55 21 1 91 144 45 99 1.07e-12 68.2
MS.gene017688.t1 AT5G18240 60.000 55 21 1 91 144 45 99 1.07e-12 68.2
MS.gene017688.t1 AT5G18240 60.000 55 21 1 91 144 45 99 1.07e-12 68.2
MS.gene017688.t1 AT1G13300 51.667 60 28 1 90 148 180 239 1.14e-12 68.2
MS.gene017688.t1 AT1G25550 51.667 60 28 1 90 148 208 267 2.20e-12 67.0
MS.gene017688.t1 AT1G49190 32.692 104 62 4 90 187 214 315 9.10e-12 65.5
MS.gene017688.t1 AT2G01060 58.182 55 22 1 91 144 15 69 1.28e-11 64.3
MS.gene017688.t1 AT1G68670 50.000 62 30 1 88 148 212 273 1.94e-11 64.3
MS.gene017688.t1 AT4G37180 50.000 60 29 1 90 148 209 268 3.56e-11 63.5
MS.gene017688.t1 AT1G49560 48.387 62 31 1 88 148 189 250 3.68e-11 63.5
MS.gene017688.t1 AT4G37180 50.000 60 29 1 90 148 216 275 4.20e-11 63.5
MS.gene017688.t1 AT3G13040 53.571 56 25 1 90 144 240 295 4.71e-11 63.5
MS.gene017688.t1 AT3G13040 53.571 56 25 1 90 144 240 295 4.71e-11 63.5
MS.gene017688.t1 AT3G13040 53.571 56 25 1 90 144 240 295 4.71e-11 63.5

Find 97 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGTCCGGTTGGACATTT+TGG 0.084147 7.1:+17468312 MS.gene017688:CDS
GGGATAGTGGTGGGTGGATT+AGG 0.144441 7.1:-17467796 None:intergenic
CAGCAGCAGCTTCATAGAAT+TGG 0.261093 7.1:+17468420 MS.gene017688:CDS
GTGATTAGGGTTTGTGATAA+AGG 0.272510 7.1:-17467736 None:intergenic
GGATAGTGGTGGGTGGATTA+GGG 0.278062 7.1:-17467795 None:intergenic
TGAGAGAAGATGATTCAAAT+TGG 0.306698 7.1:+17467668 MS.gene017688:CDS
TGGCGAGATCAGGTGTGATT+AGG 0.327778 7.1:-17467750 None:intergenic
TCCGGTTGCGCGGCCGAATT+CGG 0.334342 7.1:+17468227 MS.gene017688:CDS
TTCAGTTGAATCGGCGAAAT+CGG 0.335433 7.1:-17467869 None:intergenic
TGGTGGTTATGTGGAGGATA+TGG 0.336921 7.1:+17468488 MS.gene017688:CDS
AGCAGCAGCTTCATAGAATT+GGG 0.346797 7.1:+17468421 MS.gene017688:CDS
CGCATGCAGGGTATTTCTTC+CGG 0.354356 7.1:+17468108 MS.gene017688:CDS
GGCGAGATCAGGTGTGATTA+GGG 0.364114 7.1:-17467749 None:intergenic
GTGGAGGAAGGAAGAGAAGA+AGG 0.372281 7.1:-17467847 None:intergenic
CGGCGTCGACGAAGCGTTTG+TGG 0.387001 7.1:-17467966 None:intergenic
TTGTTGTGGTGGTGGGATAG+TGG 0.396749 7.1:-17467809 None:intergenic
TCCGAATTCGGCCGCGCAAC+CGG 0.399183 7.1:-17468228 None:intergenic
CAATTCTATGAAGCTGCTGC+TGG 0.402280 7.1:-17468419 None:intergenic
GGTGGTGGTGGTTCCGGTGG+TGG 0.402979 7.1:+17468132 MS.gene017688:CDS
GATAAAGGCATGAGTTCTTC+TGG 0.404291 7.1:-17467721 None:intergenic
TGGTTCTTCGCCGGCGGTGC+CGG 0.408437 7.1:-17467899 None:intergenic
TGAAGAAAATGCGGCGGTAC+AGG 0.409052 7.1:-17468204 None:intergenic
GGCATAAAATGATCCGAATT+CGG 0.417026 7.1:-17468240 None:intergenic
ACCGGAACCACCACCACCAC+CGG 0.421482 7.1:-17468127 None:intergenic
TATGAAGCTGCTGCTGGTGC+TGG 0.424890 7.1:-17468413 None:intergenic
TCGAAAGCGATGGCGAGATC+AGG 0.427672 7.1:-17467760 None:intergenic
ACTTTAGGTGGTGGTGGTGG+TGG 0.427931 7.1:+17468462 MS.gene017688:CDS
GTTGTTGTTGTTGTGGTGGT+GGG 0.428602 7.1:-17467816 None:intergenic
TGGAGGAAGGAAGAGAAGAA+GGG 0.433177 7.1:-17467846 None:intergenic
CAGGTGCATCATAACACTTT+AGG 0.433744 7.1:+17468447 MS.gene017688:CDS
CATAAGCTGCATTATCGTCT+TGG 0.434900 7.1:-17468019 None:intergenic
ATTTCTTCCGGTGGTGGTGG+TGG 0.438743 7.1:+17468120 MS.gene017688:CDS
TTGTGGTGGTGGGATAGTGG+TGG 0.448450 7.1:-17467806 None:intergenic
TCAAACGCCCTCGTCTTGTA+TGG 0.450475 7.1:+17467932 MS.gene017688:CDS
TCTTTATCTTAAACGCATGC+AGG 0.456959 7.1:+17468095 MS.gene017688:CDS
CAGGGTATTTCTTCCGGTGG+TGG 0.460767 7.1:+17468114 MS.gene017688:CDS
GGTATTTCTTCCGGTGGTGG+TGG 0.460989 7.1:+17468117 MS.gene017688:CDS
CGAAGCGTTTGTGGAGCTGT+GGG 0.467151 7.1:-17467957 None:intergenic
GTTCTGACTTTGTTTCCAAC+CGG 0.469745 7.1:+17468540 MS.gene017688:CDS
GTCGACGCCGTGGCGCATCT+CGG 0.482986 7.1:+17467979 MS.gene017688:CDS
ATGGAATCAGCTAATGCAAC+CGG 0.487862 7.1:+17468507 MS.gene017688:CDS
ATAAGCTGCATTATCGTCTT+GGG 0.488899 7.1:-17468018 None:intergenic
AACCCAAATTCAATCATCTC+CGG 0.488992 7.1:-17468559 None:intergenic
TGATGATGAATAGCAGGAAC+AGG 0.492936 7.1:-17468270 None:intergenic
AACACTTTAGGTGGTGGTGG+TGG 0.493430 7.1:+17468459 MS.gene017688:CDS
GGATTATCAAAATGTCCATT+CGG 0.494489 7.1:-17468342 None:intergenic
GAGGAAGGAAGAGAAGAAGG+GGG 0.497445 7.1:-17467844 None:intergenic
GGCATGAGTTCTTCTGGTGG+TGG 0.507634 7.1:-17467715 None:intergenic
GGCGACCCAAAATGTCCAAC+CGG 0.507671 7.1:-17468318 None:intergenic
TGCTGCTGATGATGAATAGC+AGG 0.508146 7.1:-17468276 None:intergenic
TAATGCAACCGGAGGGAGAA+AGG 0.508295 7.1:+17468518 MS.gene017688:CDS
GGAGGTGAGTTCAGTTGAAT+CGG 0.513901 7.1:-17467878 None:intergenic
GGAGGAAGGAAGAGAAGAAG+GGG 0.527059 7.1:-17467845 None:intergenic
AGTCAGAACCTTTCTCCCTC+CGG 0.527350 7.1:-17468526 None:intergenic
ACGAAGCGTTTGTGGAGCTG+TGG 0.532297 7.1:-17467958 None:intergenic
TGAATCGGCGAAATCGGTGG+AGG 0.543830 7.1:-17467863 None:intergenic
TTCTTCGCCGGCGGTGCCGG+AGG 0.545793 7.1:-17467896 None:intergenic
GAACTCACCTCCGGCACCGC+CGG 0.547744 7.1:+17467889 MS.gene017688:CDS
TGTGGTGGTGGGATAGTGGT+GGG 0.548736 7.1:-17467805 None:intergenic
GCTTCATAGAATTGGGGCAC+AGG 0.550758 7.1:+17468428 MS.gene017688:CDS
CATAACACTTTAGGTGGTGG+TGG 0.551977 7.1:+17468456 MS.gene017688:CDS
GATGGCTCAGTATCATCGTC+CGG 0.554969 7.1:+17468299 MS.gene017688:CDS
AGTTGAATCGGCGAAATCGG+TGG 0.557119 7.1:-17467866 None:intergenic
GAAGAAAATGCGGCGGTACA+GGG 0.558776 7.1:-17468203 None:intergenic
GGGCGTTTGAGTGTGCGAGC+TGG 0.560084 7.1:-17467919 None:intergenic
CTTTATCTTAAACGCATGCA+GGG 0.564649 7.1:+17468096 MS.gene017688:CDS
TGTGCGAGCTGGTTCTTCGC+CGG 0.569228 7.1:-17467908 None:intergenic
TCGGCGAAATCGGTGGAGGA+AGG 0.569986 7.1:-17467859 None:intergenic
ATAGCAGGAACAGGAACAAA+CGG 0.570737 7.1:-17468261 None:intergenic
CATCATAACACTTTAGGTGG+TGG 0.574928 7.1:+17468453 MS.gene017688:CDS
AACAGAAGCAGAACCACCAC+CGG 0.574953 7.1:-17468145 None:intergenic
GCATGAGTTCTTCTGGTGGT+GGG 0.580278 7.1:-17467714 None:intergenic
GCTCAGTATCATCGTCCGGT+TGG 0.583168 7.1:+17468303 MS.gene017688:CDS
GTTTCGTATGTCGAAAGCGA+TGG 0.584109 7.1:-17467770 None:intergenic
AAGAAAATGCGGCGGTACAG+GGG 0.587168 7.1:-17468202 None:intergenic
ATGCAGGGTATTTCTTCCGG+TGG 0.591915 7.1:+17468111 MS.gene017688:CDS
CAAATTCAATCATCTCCGGT+TGG 0.598529 7.1:-17468555 None:intergenic
GGTGGTGGGATAGTGGTGGG+TGG 0.599530 7.1:-17467802 None:intergenic
ATGCAGCTTATGAGTGTCGA+CGG 0.600961 7.1:+17468030 MS.gene017688:CDS
TATTCATCATCAGCAGCAGA+TGG 0.603490 7.1:+17468281 MS.gene017688:CDS
GCGAGCTGGTTCTTCGCCGG+CGG 0.605171 7.1:-17467905 None:intergenic
TGGTGGTGGTGGTTATGTGG+AGG 0.625699 7.1:+17468482 MS.gene017688:CDS
CGGTTTAACGAGAGAAAACG+TGG 0.626632 7.1:+17468050 MS.gene017688:CDS
CTTGATACCGAGATGCGCCA+CGG 0.632900 7.1:-17467986 None:intergenic
GAATCAGCTAATGCAACCGG+AGG 0.639788 7.1:+17468510 MS.gene017688:CDS
GATTCAACTGAACTCACCTC+CGG 0.640353 7.1:+17467880 MS.gene017688:CDS
GCAGCAGCTTCATAGAATTG+GGG 0.666624 7.1:+17468422 MS.gene017688:CDS
GTGCATCATAACACTTTAGG+TGG 0.672948 7.1:+17468450 MS.gene017688:CDS
TGTGGGGTCCATACAAGACG+AGG 0.673250 7.1:-17467940 None:intergenic
CAAACGCTTCGTCGACGCCG+TGG 0.674680 7.1:+17467969 MS.gene017688:CDS
ACCGGATGAAGAAAATGCGG+CGG 0.695667 7.1:-17468210 None:intergenic
GAAGCGTTTGTGGAGCTGTG+GGG 0.695777 7.1:-17467956 None:intergenic
TTATCAAAATGTCCATTCGG+CGG 0.700387 7.1:-17468339 None:intergenic
AATCAGCTAATGCAACCGGA+GGG 0.707357 7.1:+17468511 MS.gene017688:CDS
GTGGGGTCCATACAAGACGA+GGG 0.708697 7.1:-17467939 None:intergenic
GCAACCGGATGAAGAAAATG+CGG 0.719489 7.1:-17468213 None:intergenic
AAAGGCATGAGTTCTTCTGG+TGG 0.761512 7.1:-17467718 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTCAAATTGGTTTTCAAAA+TGG + chr7.1:17467680-17467699 MS.gene017688:CDS 20.0%
!!! TTCAAATTGGTTTTCAAAAT+GGG + chr7.1:17467681-17467700 MS.gene017688:CDS 20.0%
GGATTATCAAAATGTCCATT+CGG - chr7.1:17468345-17468364 None:intergenic 30.0%
TGAGAGAAGATGATTCAAAT+TGG + chr7.1:17467668-17467687 MS.gene017688:CDS 30.0%
ATAAGCTGCATTATCGTCTT+GGG - chr7.1:17468021-17468040 None:intergenic 35.0%
CTTTATCTTAAACGCATGCA+GGG + chr7.1:17468096-17468115 MS.gene017688:CDS 35.0%
GGCATAAAATGATCCGAATT+CGG - chr7.1:17468243-17468262 None:intergenic 35.0%
GTGATTAGGGTTTGTGATAA+AGG - chr7.1:17467739-17467758 None:intergenic 35.0%
TCTTTATCTTAAACGCATGC+AGG + chr7.1:17468095-17468114 MS.gene017688:CDS 35.0%
TGTTGCTGTCTAGACAAAAA+AGG - chr7.1:17468366-17468385 None:intergenic 35.0%
TTATCAAAATGTCCATTCGG+CGG - chr7.1:17468342-17468361 None:intergenic 35.0%
AGCAGCAGCTTCATAGAATT+GGG + chr7.1:17468421-17468440 MS.gene017688:CDS 40.0%
ATAGCAGGAACAGGAACAAA+CGG - chr7.1:17468264-17468283 None:intergenic 40.0%
ATGGAATCAGCTAATGCAAC+CGG + chr7.1:17468507-17468526 MS.gene017688:CDS 40.0%
CAGGTGCATCATAACACTTT+AGG + chr7.1:17468447-17468466 MS.gene017688:CDS 40.0%
CATAAGCTGCATTATCGTCT+TGG - chr7.1:17468022-17468041 None:intergenic 40.0%
CATCATAACACTTTAGGTGG+TGG + chr7.1:17468453-17468472 MS.gene017688:CDS 40.0%
GTGCATCATAACACTTTAGG+TGG + chr7.1:17468450-17468469 MS.gene017688:CDS 40.0%
TATTCATCATCAGCAGCAGA+TGG + chr7.1:17468281-17468300 MS.gene017688:CDS 40.0%
TTCAGTTGAATCGGCGAAAT+CGG - chr7.1:17467872-17467891 None:intergenic 40.0%
! GATAAAGGCATGAGTTCTTC+TGG - chr7.1:17467724-17467743 None:intergenic 40.0%
! GTTCTGACTTTGTTTCCAAC+CGG + chr7.1:17468540-17468559 MS.gene017688:CDS 40.0%
! TGATGATGAATAGCAGGAAC+AGG - chr7.1:17468273-17468292 None:intergenic 40.0%
AATCAGCTAATGCAACCGGA+GGG + chr7.1:17468511-17468530 MS.gene017688:CDS 45.0%
CAATTCTATGAAGCTGCTGC+TGG - chr7.1:17468422-17468441 None:intergenic 45.0%
CAGCAGCAGCTTCATAGAAT+TGG + chr7.1:17468420-17468439 MS.gene017688:CDS 45.0%
CGGTTTAACGAGAGAAAACG+TGG + chr7.1:17468050-17468069 MS.gene017688:CDS 45.0%
GATTCAACTGAACTCACCTC+CGG + chr7.1:17467880-17467899 MS.gene017688:CDS 45.0%
GCAACCGGATGAAGAAAATG+CGG - chr7.1:17468216-17468235 None:intergenic 45.0%
GCAGCAGCTTCATAGAATTG+GGG + chr7.1:17468422-17468441 MS.gene017688:CDS 45.0%
GGAGGTGAGTTCAGTTGAAT+CGG - chr7.1:17467881-17467900 None:intergenic 45.0%
GTTTCGTATGTCGAAAGCGA+TGG - chr7.1:17467773-17467792 None:intergenic 45.0%
TGGAGGAAGGAAGAGAAGAA+GGG - chr7.1:17467849-17467868 None:intergenic 45.0%
TGGTGGTTATGTGGAGGATA+TGG + chr7.1:17468488-17468507 MS.gene017688:CDS 45.0%
! AAAGGCATGAGTTCTTCTGG+TGG - chr7.1:17467721-17467740 None:intergenic 45.0%
! ATCGTCCGGTTGGACATTTT+GGG + chr7.1:17468313-17468332 MS.gene017688:CDS 45.0%
! ATTTTCTTCATCCGGTTGCG+CGG + chr7.1:17468217-17468236 MS.gene017688:CDS 45.0%
! CATAACACTTTAGGTGGTGG+TGG + chr7.1:17468456-17468475 MS.gene017688:CDS 45.0%
! GGTGGTTGTTGTTGTTGTTG+TGG - chr7.1:17467826-17467845 None:intergenic 45.0%
! GGTTGTTGTTGTTGTTGTGG+TGG - chr7.1:17467823-17467842 None:intergenic 45.0%
! GTTGTTGTTGTTGTGGTGGT+GGG - chr7.1:17467819-17467838 None:intergenic 45.0%
! TGCTGCTGATGATGAATAGC+AGG - chr7.1:17468279-17468298 None:intergenic 45.0%
! TGTTGTTGTTGTTGTGGTGG+TGG - chr7.1:17467820-17467839 None:intergenic 45.0%
!! ATGCAGCTTATGAGTGTCGA+CGG + chr7.1:17468030-17468049 MS.gene017688:CDS 45.0%
AACAGAAGCAGAACCACCAC+CGG - chr7.1:17468148-17468167 None:intergenic 50.0%
AAGAAAATGCGGCGGTACAG+GGG - chr7.1:17468205-17468224 None:intergenic 50.0%
ACCGGATGAAGAAAATGCGG+CGG - chr7.1:17468213-17468232 None:intergenic 50.0%
AGTCAGAACCTTTCTCCCTC+CGG - chr7.1:17468529-17468548 None:intergenic 50.0%
AGTTGAATCGGCGAAATCGG+TGG - chr7.1:17467869-17467888 None:intergenic 50.0%
GAAGAAAATGCGGCGGTACA+GGG - chr7.1:17468206-17468225 None:intergenic 50.0%
GAATCAGCTAATGCAACCGG+AGG + chr7.1:17468510-17468529 MS.gene017688:CDS 50.0%
GAGGAAGGAAGAGAAGAAGG+GGG - chr7.1:17467847-17467866 None:intergenic 50.0%
GATGGCTCAGTATCATCGTC+CGG + chr7.1:17468299-17468318 MS.gene017688:CDS 50.0%
GCATGAGTTCTTCTGGTGGT+GGG - chr7.1:17467717-17467736 None:intergenic 50.0%
GCTTCATAGAATTGGGGCAC+AGG + chr7.1:17468428-17468447 MS.gene017688:CDS 50.0%
GGAGGAAGGAAGAGAAGAAG+GGG - chr7.1:17467848-17467867 None:intergenic 50.0%
GGCGAGATCAGGTGTGATTA+GGG - chr7.1:17467752-17467771 None:intergenic 50.0%
GTGGAGGAAGGAAGAGAAGA+AGG - chr7.1:17467850-17467869 None:intergenic 50.0%
TAATGCAACCGGAGGGAGAA+AGG + chr7.1:17468518-17468537 MS.gene017688:CDS 50.0%
TCAAACGCCCTCGTCTTGTA+TGG + chr7.1:17467932-17467951 MS.gene017688:CDS 50.0%
TGAAGAAAATGCGGCGGTAC+AGG - chr7.1:17468207-17468226 None:intergenic 50.0%
TGGCGAGATCAGGTGTGATT+AGG - chr7.1:17467753-17467772 None:intergenic 50.0%
! ACCGCCGCATTTTCTTCATC+CGG + chr7.1:17468209-17468228 MS.gene017688:CDS 50.0%
! ATGCAGGGTATTTCTTCCGG+TGG + chr7.1:17468111-17468130 MS.gene017688:CDS 50.0%
! CATCGTCCGGTTGGACATTT+TGG + chr7.1:17468312-17468331 MS.gene017688:CDS 50.0%
! CGCATGCAGGGTATTTCTTC+CGG + chr7.1:17468108-17468127 MS.gene017688:CDS 50.0%
!! AACACTTTAGGTGGTGGTGG+TGG + chr7.1:17468459-17468478 MS.gene017688:CDS 50.0%
!! GGATAGTGGTGGGTGGATTA+GGG - chr7.1:17467798-17467817 None:intergenic 50.0%
!! TTGTTGTGGTGGTGGGATAG+TGG - chr7.1:17467812-17467831 None:intergenic 50.0%
CTTGATACCGAGATGCGCCA+CGG - chr7.1:17467989-17468008 None:intergenic 55.0%
GAAGGAAGAGAAGAAGGGGG+TGG - chr7.1:17467844-17467863 None:intergenic 55.0%
GCTCAGTATCATCGTCCGGT+TGG + chr7.1:17468303-17468322 MS.gene017688:CDS 55.0%
GGCATGAGTTCTTCTGGTGG+TGG - chr7.1:17467718-17467737 None:intergenic 55.0%
GGCGACCCAAAATGTCCAAC+CGG - chr7.1:17468321-17468340 None:intergenic 55.0%
GTGGGGTCCATACAAGACGA+GGG - chr7.1:17467942-17467961 None:intergenic 55.0%
TATGAAGCTGCTGCTGGTGC+TGG - chr7.1:17468416-17468435 None:intergenic 55.0%
TCGAAAGCGATGGCGAGATC+AGG - chr7.1:17467763-17467782 None:intergenic 55.0%
TGAATCGGCGAAATCGGTGG+AGG - chr7.1:17467866-17467885 None:intergenic 55.0%
TGTGGGGTCCATACAAGACG+AGG - chr7.1:17467943-17467962 None:intergenic 55.0%
! ACGAAGCGTTTGTGGAGCTG+TGG - chr7.1:17467961-17467980 None:intergenic 55.0%
! CAGGGTATTTCTTCCGGTGG+TGG + chr7.1:17468114-17468133 MS.gene017688:CDS 55.0%
! CGAAGCGTTTGTGGAGCTGT+GGG - chr7.1:17467960-17467979 None:intergenic 55.0%
! GAAGCGTTTGTGGAGCTGTG+GGG - chr7.1:17467959-17467978 None:intergenic 55.0%
! GGTATTTCTTCCGGTGGTGG+TGG + chr7.1:17468117-17468136 MS.gene017688:CDS 55.0%
!! ACTTTAGGTGGTGGTGGTGG+TGG + chr7.1:17468462-17468481 MS.gene017688:CDS 55.0%
!! ATTTCTTCCGGTGGTGGTGG+TGG + chr7.1:17468120-17468139 MS.gene017688:CDS 55.0%
!! GGGATAGTGGTGGGTGGATT+AGG - chr7.1:17467799-17467818 None:intergenic 55.0%
!! TGGTGGTGGTGGTGGTTATG+TGG + chr7.1:17468479-17468498 MS.gene017688:CDS 55.0%
!! TGGTGGTGGTGGTTATGTGG+AGG + chr7.1:17468482-17468501 MS.gene017688:CDS 55.0%
!! TGTGGTGGTGGGATAGTGGT+GGG - chr7.1:17467808-17467827 None:intergenic 55.0%
!! TTGTGGTGGTGGGATAGTGG+TGG - chr7.1:17467809-17467828 None:intergenic 55.0%
TCGGCGAAATCGGTGGAGGA+AGG - chr7.1:17467862-17467881 None:intergenic 60.0%
! TGTGCGAGCTGGTTCTTCGC+CGG - chr7.1:17467911-17467930 None:intergenic 60.0%
!! CATTTTGGGTCGCCGCCGAA+TGG + chr7.1:17468327-17468346 MS.gene017688:CDS 60.0%
!! TTAGGTGGTGGTGGTGGTGG+TGG + chr7.1:17468465-17468484 MS.gene017688:CDS 60.0%
ACCGGAACCACCACCACCAC+CGG - chr7.1:17468130-17468149 None:intergenic 65.0%
CAAACGCTTCGTCGACGCCG+TGG + chr7.1:17467969-17467988 MS.gene017688:CDS 65.0%
GGGCGTTTGAGTGTGCGAGC+TGG - chr7.1:17467922-17467941 None:intergenic 65.0%
TCCGAATTCGGCCGCGCAAC+CGG - chr7.1:17468231-17468250 None:intergenic 65.0%
TCCGGTTGCGCGGCCGAATT+CGG + chr7.1:17468227-17468246 MS.gene017688:CDS 65.0%
!! CGGCGTCGACGAAGCGTTTG+TGG - chr7.1:17467969-17467988 None:intergenic 65.0%
!! GGTGGTGGGATAGTGGTGGG+TGG - chr7.1:17467805-17467824 None:intergenic 65.0%
!! TCCGGTGGTGGTGGTGGTTC+CGG + chr7.1:17468126-17468145 MS.gene017688:CDS 65.0%
GAACTCACCTCCGGCACCGC+CGG + chr7.1:17467889-17467908 MS.gene017688:CDS 70.0%
GTCGACGCCGTGGCGCATCT+CGG + chr7.1:17467979-17467998 MS.gene017688:CDS 70.0%
TGGTTCTTCGCCGGCGGTGC+CGG - chr7.1:17467902-17467921 None:intergenic 70.0%
! GCGAGCTGGTTCTTCGCCGG+CGG - chr7.1:17467908-17467927 None:intergenic 70.0%
!! GGTGGTGGTGGTGGTGGTGG+TGG + chr7.1:17468468-17468487 MS.gene017688:CDS 70.0%
!! GGTGGTGGTGGTGGTTCCGG+TGG + chr7.1:17468129-17468148 MS.gene017688:CDS 70.0%
!! GGTGGTGGTGGTTCCGGTGG+TGG + chr7.1:17468132-17468151 MS.gene017688:CDS 70.0%
! TTCTTCGCCGGCGGTGCCGG+AGG - chr7.1:17467899-17467918 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 17467667 17468572 17467667 ID=MS.gene017688
chr7.1 mRNA 17467667 17468572 17467667 ID=MS.gene017688.t1;Parent=MS.gene017688
chr7.1 exon 17467667 17468572 17467667 ID=MS.gene017688.t1.exon1;Parent=MS.gene017688.t1
chr7.1 CDS 17467667 17468572 17467667 ID=cds.MS.gene017688.t1;Parent=MS.gene017688.t1
Gene Sequence

>MS.gene017688

ATGAGAGAAGATGATTCAAATTGGTTTTCAAAATGGGAAGAACAACTCCCACCACCAGAAGAACTCATGCCTTTATCACAAACCCTAATCACACCTGATCTCGCCATCGCTTTCGACATACGAAACAACCCTAATCCACCCACCACTATCCCACCACCACAACAACAACAACAACCACCCCCTTCTTCTCTTCCTTCCTCCACCGATTTCGCCGATTCAACTGAACTCACCTCCGGCACCGCCGGCGAAGAACCAGCTCGCACACTCAAACGCCCTCGTCTTGTATGGACCCCACAGCTCCACAAACGCTTCGTCGACGCCGTGGCGCATCTCGGTATCAAGAACGCTGTTCCCAAGACGATAATGCAGCTTATGAGTGTCGACGGTTTAACGAGAGAAAACGTGGCGAGTCATCTTCAGAAATATCGTCTTTATCTTAAACGCATGCAGGGTATTTCTTCCGGTGGTGGTGGTGGTTCCGGTGGTGGTTCTGCTTCTGTTAATGATGCTGCTACTGATCATTTATTTGCTAGCTCCCCTGTACCGCCGCATTTTCTTCATCCGGTTGCGCGGCCGAATTCGGATCATTTTATGCCGTTTGTTCCTGTTCCTGCTATTCATCATCAGCAGCAGATGGCTCAGTATCATCGTCCGGTTGGACATTTTGGGTCGCCGCCGAATGGACATTTTGATAATCCTTTTTTGTCTAGACAGCAACATCATCAACAGCAAAATCAGCATCAACACCAGCACCAGCAGCAGCTTCATAGAATTGGGGCACAGGTGCATCATAACACTTTAGGTGGTGGTGGTGGTGGTGGTGGTGGTTATGTGGAGGATATGGAATCAGCTAATGCAACCGGAGGGAGAAAGGTTCTGACTTTGTTTCCAACCGGAGATGATTGA
Protein sequence

>MS.gene017688.t1

MREDDSNWFSKWEEQLPPPEELMPLSQTLITPDLAIAFDIRNNPNPPTTIPPPQQQQQPPPSSLPSSTDFADSTELTSGTAGEEPARTLKRPRLVWTPQLHKRFVDAVAHLGIKNAVPKTIMQLMSVDGLTRENVASHLQKYRLYLKRMQGISSGGGGGSGGGSASVNDAATDHLFASSPVPPHFLHPVARPNSDHFMPFVPVPAIHHQQQMAQYHRPVGHFGSPPNGHFDNPFLSRQQHHQQQNQHQHQHQQQLHRIGAQVHHNTLGGGGGGGGGYVEDMESANATGGRKVLTLFPTGDD