AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene017699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017699.t1 MTR_7g089190 96.476 454 14 1 1 454 1 452 0.0 916
MS.gene017699.t1 MTR_1g044120 34.629 283 156 6 195 449 463 744 3.91e-45 169
MS.gene017699.t1 MTR_2g084630 27.198 489 265 12 53 453 158 643 1.10e-44 168
MS.gene017699.t1 MTR_2g084630 27.443 481 268 11 53 453 158 637 1.11e-44 168
MS.gene017699.t1 MTR_2g012830 27.374 358 143 8 188 454 536 867 3.60e-35 140
MS.gene017699.t1 MTR_5g067980 32.768 177 91 4 296 444 487 663 2.16e-20 95.5
MS.gene017699.t1 MTR_4g118845 31.868 182 96 6 253 428 209 368 1.44e-18 89.4
MS.gene017699.t1 MTR_4g118845 31.868 182 96 6 253 428 22 181 1.47e-18 89.4
MS.gene017699.t1 MTR_2g020000 30.939 181 99 5 253 428 212 371 2.58e-17 85.5
MS.gene017699.t1 MTR_4g035100 29.534 193 117 5 253 441 1471 1648 3.33e-17 85.5
MS.gene017699.t1 MTR_4g035100 29.534 193 117 5 253 441 1471 1648 3.48e-17 85.5
MS.gene017699.t1 MTR_4g078460 24.074 270 163 7 191 458 405 634 4.09e-17 84.7
MS.gene017699.t1 MTR_4g078495 25.185 270 160 9 191 458 119 348 4.42e-17 84.7
MS.gene017699.t1 MTR_4g078460 24.074 270 163 7 191 458 405 634 4.70e-17 84.7
MS.gene017699.t1 MTR_1g115215 38.889 90 55 0 365 454 522 611 2.76e-16 82.4
MS.gene017699.t1 MTR_1g053955 32.955 176 90 7 253 422 542 695 6.86e-16 81.3
MS.gene017699.t1 MTR_1g035330 27.041 196 106 6 250 428 214 389 3.22e-15 78.6
MS.gene017699.t1 MTR_4g049500 31.667 120 78 1 252 367 260 379 6.96e-15 77.8
MS.gene017699.t1 MTR_4g049500 47.458 59 31 0 370 428 455 513 2.75e-11 66.6
MS.gene017699.t1 MTR_7g090960 28.804 184 93 6 253 422 599 758 1.21e-14 77.4
MS.gene017699.t1 MTR_7g090960 28.804 184 93 6 253 422 518 677 1.29e-14 77.0
MS.gene017699.t1 MTR_4g118720 28.111 217 130 7 253 463 1026 1222 6.42e-14 75.1
MS.gene017699.t1 MTR_5g005840 30.857 175 96 5 253 422 384 538 6.81e-14 74.7
MS.gene017699.t1 MTR_5g020000 26.738 187 102 6 253 428 205 367 8.06e-14 74.3
MS.gene017699.t1 MTR_3g053910 28.421 190 101 5 254 430 622 789 1.49e-13 73.9
MS.gene017699.t1 MTR_3g053910 28.421 190 101 5 254 430 593 760 1.55e-13 73.9
MS.gene017699.t1 MTR_5g090090 27.083 192 101 6 254 431 639 805 5.60e-13 72.0
MS.gene017699.t1 MTR_5g090090 27.083 192 101 6 254 431 639 805 5.90e-13 72.0
MS.gene017699.t1 MTR_7g039320 26.939 245 101 11 199 421 35 223 1.85e-12 70.1
MS.gene017699.t1 MTR_7g039320 26.939 245 101 11 199 421 35 223 1.94e-12 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017699.t1 AT5G05130 56.646 489 162 7 1 454 42 515 0.0 535
MS.gene017699.t1 AT5G22750 35.738 305 144 6 201 453 346 650 7.90e-45 168
MS.gene017699.t1 AT5G43530 33.670 297 163 9 190 453 604 899 9.61e-44 165
MS.gene017699.t1 AT3G16600 31.338 284 138 5 195 453 45 296 3.94e-40 153
MS.gene017699.t1 AT1G11100 31.633 294 142 8 193 454 389 655 4.24e-37 145
MS.gene017699.t1 AT1G11100 31.633 294 142 8 193 454 532 798 5.32e-37 145
MS.gene017699.t1 AT1G61140 27.143 350 139 7 195 454 390 713 4.34e-34 136
MS.gene017699.t1 AT1G61140 27.143 350 139 7 195 454 555 878 5.31e-34 136
MS.gene017699.t1 AT1G61140 27.143 350 139 7 195 454 548 871 5.80e-34 136
MS.gene017699.t1 AT1G02670 37.433 187 97 4 250 428 142 316 1.03e-32 130
MS.gene017699.t1 AT1G02670 37.433 187 97 4 250 428 115 289 1.80e-32 130
MS.gene017699.t1 AT1G02670 37.433 187 97 4 250 428 115 289 1.94e-32 131
MS.gene017699.t1 AT1G02670 37.838 185 95 4 252 428 144 316 2.72e-32 130
MS.gene017699.t1 AT1G02670 37.838 185 95 4 252 428 144 316 3.03e-32 130
MS.gene017699.t1 AT1G11100 27.811 338 141 8 193 454 532 842 5.49e-32 130
MS.gene017699.t1 AT1G02670 37.705 183 92 5 252 424 144 314 3.77e-30 124
MS.gene017699.t1 AT1G02670 37.705 183 92 5 252 424 155 325 4.12e-30 124
MS.gene017699.t1 AT2G40770 32.597 181 95 3 296 449 499 679 2.87e-23 104
MS.gene017699.t1 AT2G40770 32.597 181 95 3 296 449 499 679 2.87e-23 104
MS.gene017699.t1 AT2G40770 32.597 181 95 3 296 449 499 679 2.87e-23 104
MS.gene017699.t1 AT3G54280 29.319 191 120 4 253 441 1503 1680 3.11e-19 92.0
MS.gene017699.t1 AT3G54280 29.319 191 120 4 253 441 1472 1649 3.40e-19 91.7
MS.gene017699.t1 AT1G03750 25.490 255 147 7 195 447 132 345 5.39e-19 90.9
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 245 335 1.82e-18 89.0
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 270 360 2.19e-18 88.6
MS.gene017699.t1 AT1G05120 30.769 182 82 5 187 366 124 263 2.58e-18 88.6
MS.gene017699.t1 AT1G05120 50.847 59 29 0 370 428 354 412 3.80e-12 69.3
MS.gene017699.t1 AT1G05120 30.769 182 82 5 187 366 167 306 2.70e-18 88.6
MS.gene017699.t1 AT1G05120 49.180 61 31 0 368 428 395 455 4.13e-12 68.9
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 536 626 3.83e-18 88.2
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 536 626 3.83e-18 88.2
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 536 626 3.83e-18 88.2
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 536 626 4.76e-18 87.8
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 536 626 4.76e-18 87.8
MS.gene017699.t1 AT3G20010 41.758 91 53 0 364 454 536 626 4.76e-18 87.8
MS.gene017699.t1 AT5G18620 32.967 182 94 7 253 428 215 374 3.63e-17 85.1
MS.gene017699.t1 AT5G18620 32.967 182 94 7 253 428 215 374 3.63e-17 85.1
MS.gene017699.t1 AT3G06400 32.418 182 95 7 253 428 210 369 8.22e-17 84.0
MS.gene017699.t1 AT3G06400 32.418 182 95 7 253 428 210 369 8.44e-17 84.0
MS.gene017699.t1 AT3G06400 32.418 182 95 7 253 428 210 369 8.75e-17 84.0
MS.gene017699.t1 AT1G50410 27.652 264 101 9 195 406 218 443 2.64e-16 82.4
MS.gene017699.t1 AT1G50410 40.000 90 54 0 365 454 466 555 2.81e-16 82.4
MS.gene017699.t1 AT1G50410 40.000 90 54 0 365 454 466 555 3.25e-16 82.0
MS.gene017699.t1 AT1G50410 27.652 264 101 9 195 406 218 443 3.46e-16 82.0
MS.gene017699.t1 AT1G50410 40.000 90 54 0 365 454 466 555 3.25e-16 82.0
MS.gene017699.t1 AT1G50410 27.652 264 101 9 195 406 218 443 3.46e-16 82.0
MS.gene017699.t1 AT1G50410 40.000 90 54 0 365 454 466 555 3.25e-16 82.0
MS.gene017699.t1 AT1G50410 27.652 264 101 9 195 406 218 443 3.46e-16 82.0
MS.gene017699.t1 AT3G12810 31.818 176 92 6 253 422 553 706 4.63e-16 82.0
MS.gene017699.t1 AT3G12810 31.818 176 92 6 253 422 557 710 4.67e-16 82.0
MS.gene017699.t1 AT1G11100 39.560 91 55 0 364 454 751 841 2.66e-15 79.3
MS.gene017699.t1 AT1G11100 39.560 91 55 0 364 454 609 699 2.82e-15 79.3
MS.gene017699.t1 AT3G54460 28.571 161 107 4 296 448 456 616 3.04e-15 79.0
MS.gene017699.t1 AT3G54460 28.571 161 107 4 296 448 753 913 3.74e-15 79.0
MS.gene017699.t1 AT2G02090 27.461 193 109 6 250 428 232 407 1.32e-14 77.0
MS.gene017699.t1 AT2G28290 29.487 234 110 9 195 422 746 930 3.91e-14 75.9
MS.gene017699.t1 AT2G28290 29.487 234 110 9 195 422 747 931 3.98e-14 75.9
MS.gene017699.t1 AT2G28290 29.487 234 110 9 195 422 746 930 4.01e-14 75.9
MS.gene017699.t1 AT2G28290 29.487 234 110 9 195 422 746 930 4.01e-14 75.9
MS.gene017699.t1 AT2G28290 29.487 234 110 9 195 422 747 931 4.15e-14 75.9
MS.gene017699.t1 AT2G28290 29.060 234 111 9 195 422 746 930 4.26e-14 75.9
MS.gene017699.t1 AT3G06010 27.074 229 120 9 253 468 424 618 4.96e-13 72.0
MS.gene017699.t1 AT5G66750 26.667 195 106 6 253 435 223 392 2.83e-12 69.7
MS.gene017699.t1 AT3G57300 26.882 186 94 6 253 422 607 766 4.21e-12 69.3
MS.gene017699.t1 AT4G31900 28.095 210 111 7 254 453 206 385 4.35e-12 69.3
MS.gene017699.t1 AT4G31900 28.095 210 111 7 254 453 247 426 4.59e-12 68.9
MS.gene017699.t1 AT4G31900 28.095 210 111 7 254 453 247 426 5.04e-12 68.9
MS.gene017699.t1 AT3G57300 26.882 186 94 6 253 422 607 766 5.56e-12 68.9
MS.gene017699.t1 AT3G57300 26.882 186 94 6 253 422 607 766 5.56e-12 68.9
MS.gene017699.t1 AT2G13370 26.178 191 104 5 254 430 647 814 8.89e-12 68.2
MS.gene017699.t1 AT2G13370 26.178 191 104 5 254 430 647 814 8.89e-12 68.2
MS.gene017699.t1 AT2G13370 26.178 191 104 5 254 430 647 814 8.89e-12 68.2
MS.gene017699.t1 AT5G19310 26.126 222 130 8 253 468 143 336 1.17e-11 67.8
MS.gene017699.t1 AT5G19310 28.977 176 97 7 253 422 407 560 1.46e-11 67.4
MS.gene017699.t1 AT2G44980 28.729 181 91 8 257 421 77 235 2.37e-11 66.6

Find 129 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGAAGGTTTATATGTATTA+TGG 0.243058 7.2:+19124944 MS.gene017699:CDS
TTTGCGGACGGGATGGGATT+AGG 0.262324 7.2:+19124737 MS.gene017699:CDS
CTGATGATTTACCGCCGTTT+TGG 0.262594 7.2:+19124633 MS.gene017699:CDS
GGATACTCCTGTGAAGAAAT+TGG 0.280994 7.2:+19125057 MS.gene017699:CDS
TTGGAGTCGTATGGGTTTAA+AGG 0.302377 7.2:-19124113 None:intergenic
GAAACCGGCGAGGTAGGTTT+CGG 0.351950 7.2:-19124012 None:intergenic
TTGCGGACGGGATGGGATTA+GGG 0.354547 7.2:+19124738 MS.gene017699:CDS
GATACTCCTGTGAAGAAATT+GGG 0.364943 7.2:+19125058 MS.gene017699:CDS
GGTAGGTTTCGGATTGGGAT+TGG 0.365722 7.2:-19124001 None:intergenic
CGAGTTTGAAAATTTGATCT+AGG 0.366948 7.2:-19124484 None:intergenic
CGGTTCAGAAACTAGGGTTT+TGG 0.367071 7.2:-19124525 None:intergenic
TTTATATGTATTATGGTGAT+AGG 0.371908 7.2:+19124951 MS.gene017699:CDS
GATAAAATGAAAATGAAAAG+TGG 0.372811 7.2:+19124794 MS.gene017699:CDS
TGCGCGGCCGGAGCCTCTCT+CGG 0.374792 7.2:+19124706 MS.gene017699:CDS
GGGGTGGAGGAGGATTGTTT+TGG 0.395962 7.2:+19125078 MS.gene017699:CDS
GTAGGTTTCGGATTGGGATT+GGG 0.396664 7.2:-19124000 None:intergenic
TGTTAATGCGGGACAGAGTC+AGG 0.398787 7.2:+19125123 MS.gene017699:CDS
GGCGAGGTAGGTTTCGGATT+GGG 0.403200 7.2:-19124006 None:intergenic
AAGAGGAGATGGGCTGTTAC+GGG 0.409943 7.2:+19125166 MS.gene017699:CDS
TTCGACATGGATAACTCAAT+TGG 0.410437 7.2:+19124898 MS.gene017699:CDS
GGTTTAACGGTTCAGAAACT+AGG 0.413284 7.2:-19124532 None:intergenic
GTCAGCGCGTGTTTCTTTAA+CGG 0.413934 7.2:-19124414 None:intergenic
GGGAGCTGAGTTGCGGTGTT+CGG 0.416851 7.2:+19125036 MS.gene017699:CDS
TTCGGATTGGGATTGGGAGA+AGG 0.428738 7.2:-19123994 None:intergenic
TCATAAGAACCATTCTGAAT+AGG 0.433793 7.2:-19125193 None:intergenic
GACCGGGATGAGTCGATTGC+AGG 0.436131 7.2:+19125312 MS.gene017699:CDS
ATGCTAGAAGGAGGGTTTAA+CGG 0.438865 7.2:-19124545 None:intergenic
TTAACGTTGCTATCGTTGAT+TGG 0.448424 7.2:+19124767 MS.gene017699:CDS
GAAGAGGAGATGGGCTGTTA+CGG 0.450082 7.2:+19125165 MS.gene017699:CDS
TTGATGGCGTTGGAGTCGTA+TGG 0.450151 7.2:-19124122 None:intergenic
CTTTGAATGCGAAGAGGAGA+TGG 0.451636 7.2:+19125155 MS.gene017699:CDS
TCTGATTTAATAATGCTAGA+AGG 0.452942 7.2:-19124557 None:intergenic
ATCCCTCCCGAGAGAGGCTC+CGG 0.453624 7.2:-19124713 None:intergenic
CAATAACTCTTAATAGAAAA+CGG 0.456175 7.2:-19125250 None:intergenic
GATGTTGGCCATTACGAAAC+CGG 0.457398 7.2:-19124027 None:intergenic
GCGCGGCCGGAGCCTCTCTC+GGG 0.458130 7.2:+19124707 MS.gene017699:CDS
GGGATATTTGCGGACGGGAT+GGG 0.460336 7.2:+19124731 MS.gene017699:CDS
TTGAGTGTTGAGGACTTTGA+TGG 0.469309 7.2:-19124138 None:intergenic
CTCCACCCCAATTTCTTCAC+AGG 0.470263 7.2:-19125064 None:intergenic
CACTACTCCGGCACCATCAC+CGG 0.474897 7.2:+19124062 MS.gene017699:CDS
TAGGGGTAGTGTAGAGAGGG+TGG 0.476445 7.2:+19124829 MS.gene017699:CDS
GAGGATTTCGCGGCCGGTGA+TGG 0.480465 7.2:-19124075 None:intergenic
TCGGATTGGGATTGGGAGAA+GGG 0.481945 7.2:-19123993 None:intergenic
TAAGCCGAGGATTTCGCGGC+CGG 0.485440 7.2:-19124081 None:intergenic
CCCTCTTTCTCCTCCCAAAA+CGG 0.487569 7.2:-19124647 None:intergenic
GTTCAGCGACCTCTTAATCA+AGG 0.488325 7.2:+19125283 MS.gene017699:CDS
TTCGCGGCCGGTGATGGTGC+CGG 0.488545 7.2:-19124069 None:intergenic
TACTAACCGTGGCACGTTGA+AGG 0.489496 7.2:+19124928 MS.gene017699:CDS
CTCAAGATGCTGAGGAGTTG+AGG 0.490043 7.2:+19124978 MS.gene017699:CDS
TAAGAGTTATTGGCAAAGCT+TGG 0.490157 7.2:+19125261 MS.gene017699:CDS
TTGACTACTTATGCTACATT+GGG 0.494687 7.2:+19125016 MS.gene017699:CDS
GTTGAGGACTTTGATGGCGT+TGG 0.504215 7.2:-19124132 None:intergenic
CGGCGAGGTAGGTTTCGGAT+TGG 0.508795 7.2:-19124007 None:intergenic
GTTGACTACTTATGCTACAT+TGG 0.511870 7.2:+19125015 MS.gene017699:CDS
AGCGCTGATTCTGTAACAAC+CGG 0.513980 7.2:+19124443 MS.gene017699:CDS
GGATTAAGCCGAGGATTTCG+CGG 0.515048 7.2:-19124085 None:intergenic
CTACCTCGCCGGTTTCGTAA+TGG 0.515519 7.2:+19124019 MS.gene017699:CDS
AGTGGGAAGAAAAGAGGTAG+GGG 0.516027 7.2:+19124812 MS.gene017699:CDS
CGCGTTCGATGTAACCGACT+TGG 0.516483 7.2:-19124166 None:intergenic
AAGTGGGAAGAAAAGAGGTA+GGG 0.519183 7.2:+19124811 MS.gene017699:CDS
AGGGATATTTGCGGACGGGA+TGG 0.519906 7.2:+19124730 MS.gene017699:CDS
TGATGGCGTTGGAGTCGTAT+GGG 0.521093 7.2:-19124121 None:intergenic
TGAAGAAATTGGGGTGGAGG+AGG 0.522236 7.2:+19125068 MS.gene017699:CDS
CGGCCGGAGCCTCTCTCGGG+AGG 0.523817 7.2:+19124710 MS.gene017699:CDS
ATAAAATGAAAATGAAAAGT+GGG 0.525475 7.2:+19124795 MS.gene017699:CDS
ATGAAAAGTGGGAAGAAAAG+AGG 0.526742 7.2:+19124806 MS.gene017699:CDS
TATGAATCTGACAAGGGATA+CGG 0.528146 7.2:-19124283 None:intergenic
ATCACCGGCCGCGAAATCCT+CGG 0.528188 7.2:+19124077 MS.gene017699:CDS
CTGTGAAGAAATTGGGGTGG+AGG 0.528357 7.2:+19125065 MS.gene017699:CDS
GTCGAGGAGAGGAGCGAGAG+CGG 0.529382 7.2:-19124198 None:intergenic
TTCGTAATGGCCAACATCGT+CGG 0.530765 7.2:+19124032 MS.gene017699:CDS
CTCCTTCTGTGATTTCGACA+TGG 0.531528 7.2:+19124885 MS.gene017699:CDS
GCGTGTTTCTTTAACGGCGG+CGG 0.532351 7.2:-19124408 None:intergenic
CTTGGAGTGTTTGAGTGTTG+AGG 0.532776 7.2:-19124148 None:intergenic
TTATCAAACTAATGCGCGGC+CGG 0.540005 7.2:+19124694 MS.gene017699:CDS
AGGTAGGGGTAGTGTAGAGA+GGG 0.545313 7.2:+19124826 MS.gene017699:CDS
ATACGAAATGAAATGAACAT+TGG 0.545733 7.2:-19124357 None:intergenic
GTGTTTAATGCCGACGATGT+TGG 0.546373 7.2:-19124042 None:intergenic
GAGGTAGGGGTAGTGTAGAG+AGG 0.546579 7.2:+19124825 MS.gene017699:CDS
AAAGTGGGAAGAAAAGAGGT+AGG 0.547195 7.2:+19124810 MS.gene017699:CDS
GTTTAACGGTTCAGAAACTA+GGG 0.551674 7.2:-19124531 None:intergenic
GGCCGGAGCCTCTCTCGGGA+GGG 0.552185 7.2:+19124711 MS.gene017699:CDS
CGTATGGGTTTAAAGGTTCG+CGG 0.555958 7.2:-19124106 None:intergenic
CTTAATCAAGGAAAACAGAC+CGG 0.556797 7.2:+19125295 MS.gene017699:CDS
TACCTGCAATCGACTCATCC+CGG 0.558747 7.2:-19125314 None:intergenic
CATTACGAAACCGGCGAGGT+AGG 0.559895 7.2:-19124018 None:intergenic
GCAAATATCCCTCCCGAGAG+AGG 0.560537 7.2:-19124719 None:intergenic
GGGTTCTTGTGAGTCTTCCA+TGG 0.560574 7.2:-19123973 None:intergenic
ACGGGGACGCCTATTCAGAA+TGG 0.563434 7.2:+19125184 MS.gene017699:CDS
GACATGGATAACTCAATTGG+AGG 0.564551 7.2:+19124901 MS.gene017699:CDS
AGCGCGTGTTTCTTTAACGG+CGG 0.569465 7.2:-19124411 None:intergenic
GCGAGTGTTGGTTTGAACGA+TGG 0.569836 7.2:-19124243 None:intergenic
ATTTAATAATGCTAGAAGGA+GGG 0.574890 7.2:-19124553 None:intergenic
GATTTAATAATGCTAGAAGG+AGG 0.575245 7.2:-19124554 None:intergenic
CGGGAGGGATATTTGCGGAC+GGG 0.579950 7.2:+19124726 MS.gene017699:CDS
GAGGAGAGGAGCGAGAGCGG+CGG 0.582518 7.2:-19124195 None:intergenic
GTTATTGTTAGAGCGAGTGT+TGG 0.586502 7.2:-19124255 None:intergenic
ATATGTATTATGGTGATAGG+AGG 0.588645 7.2:+19124954 MS.gene017699:CDS
ACGATCAAGAATGTTAATGC+GGG 0.594066 7.2:+19125112 MS.gene017699:CDS
AGGGTGGAGAGTGAGACAAA+TGG 0.602442 7.2:+19124845 MS.gene017699:CDS
TGTTACAGAATCAGCGCTGA+CGG 0.605972 7.2:-19124438 None:intergenic
TATAAACCTTCAACGTGCCA+CGG 0.606128 7.2:-19124934 None:intergenic
ATGATATGAGCGTCGAGGAG+AGG 0.615397 7.2:-19124209 None:intergenic
ATCCATGTCGAAATCACAGA+AGG 0.617581 7.2:-19124887 None:intergenic
TTTGAATGCGAAGAGGAGAT+GGG 0.618382 7.2:+19125156 MS.gene017699:CDS
GCGAAGATATGAATCTGACA+AGG 0.618949 7.2:-19124290 None:intergenic
TTAATCAAGGAAAACAGACC+GGG 0.621325 7.2:+19125296 MS.gene017699:CDS
CAATCCGAAACCTACCTCGC+CGG 0.623218 7.2:+19124008 MS.gene017699:CDS
ATACTCCTGTGAAGAAATTG+GGG 0.629443 7.2:+19125059 MS.gene017699:CDS
CTCCTGTGAAGAAATTGGGG+TGG 0.632108 7.2:+19125062 MS.gene017699:CDS
TCTCTCGGGAGGGATATTTG+CGG 0.634363 7.2:+19124721 MS.gene017699:CDS
TCGGGAGGGATATTTGCGGA+CGG 0.634657 7.2:+19124725 MS.gene017699:CDS
TACGATCAAGAATGTTAATG+CGG 0.642076 7.2:+19125111 MS.gene017699:CDS
TAGGAGGACTCAAGATGCTG+AGG 0.643252 7.2:+19124970 MS.gene017699:CDS
CTACATTGGGAGCTGAGTTG+CGG 0.644145 7.2:+19125029 MS.gene017699:CDS
AGAGGAGATGGGCTGTTACG+GGG 0.645108 7.2:+19125167 MS.gene017699:CDS
GTCGGCATTAAACACTACTC+CGG 0.651569 7.2:+19124050 MS.gene017699:CDS
AAGGTTCGCGGATTAAGCCG+AGG 0.655327 7.2:-19124094 None:intergenic
AACACTCAAACACTCCAAGT+CGG 0.656074 7.2:+19124152 MS.gene017699:CDS
CATGTCGAAATCACAGAAGG+AGG 0.664824 7.2:-19124884 None:intergenic
CGAAGATATGAATCTGACAA+GGG 0.668320 7.2:-19124289 None:intergenic
TTTGAACGATGGCTTCGACG+TGG 0.670822 7.2:-19124232 None:intergenic
TGATTGCTTTGAATGCGAAG+AGG 0.688442 7.2:+19125149 MS.gene017699:CDS
TTGGAGGAACATACTAACCG+TGG 0.701328 7.2:+19124917 MS.gene017699:CDS
TGGCCATTACGAAACCGGCG+AGG 0.702379 7.2:-19124022 None:intergenic
CGAATTATCAAACTAATGCG+CGG 0.709330 7.2:+19124690 MS.gene017699:CDS
TCTTTCTCCTCCCAAAACGG+CGG 0.713897 7.2:-19124644 None:intergenic
GAATGAGAAAGAGTAAACGA+AGG 0.718846 7.2:-19124386 None:intergenic
CGTGGATGATATGAGCGTCG+AGG 0.719430 7.2:-19124214 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAATGAAAATGAAAAGT+GGG + chr7.2:19124795-19124814 MS.gene017699:CDS 15.0%
!!! GTTTTTACTATTATTATTAC+CGG - chr7.2:19124465-19124484 None:intergenic 15.0%
!! CAATAACTCTTAATAGAAAA+CGG - chr7.2:19125253-19125272 None:intergenic 20.0%
!! GATAAAATGAAAATGAAAAG+TGG + chr7.2:19124794-19124813 MS.gene017699:CDS 20.0%
!! TTGAAGGTTTATATGTATTA+TGG + chr7.2:19124944-19124963 MS.gene017699:CDS 20.0%
!! TTTATATGTATTATGGTGAT+AGG + chr7.2:19124951-19124970 MS.gene017699:CDS 20.0%
! ATACGAAATGAAATGAACAT+TGG - chr7.2:19124360-19124379 None:intergenic 25.0%
! ATTTAATAATGCTAGAAGGA+GGG - chr7.2:19124556-19124575 None:intergenic 25.0%
! TCTGATTTAATAATGCTAGA+AGG - chr7.2:19124560-19124579 None:intergenic 25.0%
!! CGTTTTCTATTAAGAGTTAT+TGG + chr7.2:19125251-19125270 MS.gene017699:CDS 25.0%
!!! TTATGACTTGTTTTCTCTTA+TGG + chr7.2:19125210-19125229 MS.gene017699:CDS 25.0%
ATATGTATTATGGTGATAGG+AGG + chr7.2:19124954-19124973 MS.gene017699:CDS 30.0%
CGAGTTTGAAAATTTGATCT+AGG - chr7.2:19124487-19124506 None:intergenic 30.0%
GATTTAATAATGCTAGAAGG+AGG - chr7.2:19124557-19124576 None:intergenic 30.0%
TACGATCAAGAATGTTAATG+CGG + chr7.2:19125111-19125130 MS.gene017699:CDS 30.0%
TCATAAGAACCATTCTGAAT+AGG - chr7.2:19125196-19125215 None:intergenic 30.0%
TTGACTACTTATGCTACATT+GGG + chr7.2:19125016-19125035 MS.gene017699:CDS 30.0%
ACGATCAAGAATGTTAATGC+GGG + chr7.2:19125112-19125131 MS.gene017699:CDS 35.0%
ATACTCCTGTGAAGAAATTG+GGG + chr7.2:19125059-19125078 MS.gene017699:CDS 35.0%
ATGAAAAGTGGGAAGAAAAG+AGG + chr7.2:19124806-19124825 MS.gene017699:CDS 35.0%
CGAAGATATGAATCTGACAA+GGG - chr7.2:19124292-19124311 None:intergenic 35.0%
CGAATTATCAAACTAATGCG+CGG + chr7.2:19124690-19124709 MS.gene017699:CDS 35.0%
CTTAATCAAGGAAAACAGAC+CGG + chr7.2:19125295-19125314 MS.gene017699:CDS 35.0%
GAATGAGAAAGAGTAAACGA+AGG - chr7.2:19124389-19124408 None:intergenic 35.0%
GATACTCCTGTGAAGAAATT+GGG + chr7.2:19125058-19125077 MS.gene017699:CDS 35.0%
GTTGACTACTTATGCTACAT+TGG + chr7.2:19125015-19125034 MS.gene017699:CDS 35.0%
GTTTAACGGTTCAGAAACTA+GGG - chr7.2:19124534-19124553 None:intergenic 35.0%
TAAGAGTTATTGGCAAAGCT+TGG + chr7.2:19125261-19125280 MS.gene017699:CDS 35.0%
TATGAATCTGACAAGGGATA+CGG - chr7.2:19124286-19124305 None:intergenic 35.0%
TTAACGTTGCTATCGTTGAT+TGG + chr7.2:19124767-19124786 MS.gene017699:CDS 35.0%
TTAATCAAGGAAAACAGACC+GGG + chr7.2:19125296-19125315 MS.gene017699:CDS 35.0%
TTCGACATGGATAACTCAAT+TGG + chr7.2:19124898-19124917 MS.gene017699:CDS 35.0%
! GTCTGTTTTCCTTGATTAAG+AGG - chr7.2:19125295-19125314 None:intergenic 35.0%
AAAGTGGGAAGAAAAGAGGT+AGG + chr7.2:19124810-19124829 MS.gene017699:CDS 40.0%
AACACTCAAACACTCCAAGT+CGG + chr7.2:19124152-19124171 MS.gene017699:CDS 40.0%
AAGTGGGAAGAAAAGAGGTA+GGG + chr7.2:19124811-19124830 MS.gene017699:CDS 40.0%
ATCCATGTCGAAATCACAGA+AGG - chr7.2:19124890-19124909 None:intergenic 40.0%
ATGCTAGAAGGAGGGTTTAA+CGG - chr7.2:19124548-19124567 None:intergenic 40.0%
GACATGGATAACTCAATTGG+AGG + chr7.2:19124901-19124920 MS.gene017699:CDS 40.0%
GCGAAGATATGAATCTGACA+AGG - chr7.2:19124293-19124312 None:intergenic 40.0%
GGATACTCCTGTGAAGAAAT+TGG + chr7.2:19125057-19125076 MS.gene017699:CDS 40.0%
GGTTTAACGGTTCAGAAACT+AGG - chr7.2:19124535-19124554 None:intergenic 40.0%
GTTATTGTTAGAGCGAGTGT+TGG - chr7.2:19124258-19124277 None:intergenic 40.0%
TATAAACCTTCAACGTGCCA+CGG - chr7.2:19124937-19124956 None:intergenic 40.0%
TTGGAGTCGTATGGGTTTAA+AGG - chr7.2:19124116-19124135 None:intergenic 40.0%
TTTGAATGCGAAGAGGAGAT+GGG + chr7.2:19125156-19125175 MS.gene017699:CDS 40.0%
! GCTGTTCATGACGCTTTTAA+CGG + chr7.2:19124332-19124351 MS.gene017699:CDS 40.0%
! TGATTGCTTTGAATGCGAAG+AGG + chr7.2:19125149-19125168 MS.gene017699:CDS 40.0%
!! ACCAAAAAGAAGCACTAGGA+TGG + chr7.2:19124591-19124610 MS.gene017699:CDS 40.0%
!! TGATGATTTACCGCCGTTTT+GGG + chr7.2:19124634-19124653 MS.gene017699:CDS 40.0%
!! TTGAGTGTTGAGGACTTTGA+TGG - chr7.2:19124141-19124160 None:intergenic 40.0%
!!! AAACTAGGGTTTTGGAAGCT+AGG - chr7.2:19124520-19124539 None:intergenic 40.0%
AGTGGGAAGAAAAGAGGTAG+GGG + chr7.2:19124812-19124831 MS.gene017699:CDS 45.0%
CATGTCGAAATCACAGAAGG+AGG - chr7.2:19124887-19124906 None:intergenic 45.0%
CGTATGGGTTTAAAGGTTCG+CGG - chr7.2:19124109-19124128 None:intergenic 45.0%
CTCCTTCTGTGATTTCGACA+TGG + chr7.2:19124885-19124904 MS.gene017699:CDS 45.0%
CTTTGAATGCGAAGAGGAGA+TGG + chr7.2:19125155-19125174 MS.gene017699:CDS 45.0%
GATGTTGGCCATTACGAAAC+CGG - chr7.2:19124030-19124049 None:intergenic 45.0%
GTCAGCGCGTGTTTCTTTAA+CGG - chr7.2:19124417-19124436 None:intergenic 45.0%
GTCGGCATTAAACACTACTC+CGG + chr7.2:19124050-19124069 MS.gene017699:CDS 45.0%
GTGTTTAATGCCGACGATGT+TGG - chr7.2:19124045-19124064 None:intergenic 45.0%
GTTCAGCGACCTCTTAATCA+AGG + chr7.2:19125283-19125302 MS.gene017699:CDS 45.0%
TTATCAAACTAATGCGCGGC+CGG + chr7.2:19124694-19124713 MS.gene017699:CDS 45.0%
TTCGTAATGGCCAACATCGT+CGG + chr7.2:19124032-19124051 MS.gene017699:CDS 45.0%
TTGGAGGAACATACTAACCG+TGG + chr7.2:19124917-19124936 MS.gene017699:CDS 45.0%
! CGGTTCAGAAACTAGGGTTT+TGG - chr7.2:19124528-19124547 None:intergenic 45.0%
! CTGATGATTTACCGCCGTTT+TGG + chr7.2:19124633-19124652 MS.gene017699:CDS 45.0%
! CTTGGAGTGTTTGAGTGTTG+AGG - chr7.2:19124151-19124170 None:intergenic 45.0%
! GCCATCCTAGTGCTTCTTTT+TGG - chr7.2:19124595-19124614 None:intergenic 45.0%
! GTAGGTTTCGGATTGGGATT+GGG - chr7.2:19124003-19124022 None:intergenic 45.0%
! TGTTACAGAATCAGCGCTGA+CGG - chr7.2:19124441-19124460 None:intergenic 45.0%
!! AGCGCTGATTCTGTAACAAC+CGG + chr7.2:19124443-19124462 MS.gene017699:CDS 45.0%
!! CAGCACCAAAAAGAAGCACT+AGG + chr7.2:19124587-19124606 MS.gene017699:CDS 45.0%
!!! GAGGAGGATTGTTTTGGATG+AGG + chr7.2:19125084-19125103 MS.gene017699:CDS 45.0%
AGCGCGTGTTTCTTTAACGG+CGG - chr7.2:19124414-19124433 None:intergenic 50.0%
AGGGTGGAGAGTGAGACAAA+TGG + chr7.2:19124845-19124864 MS.gene017699:CDS 50.0%
AGGTAGGGGTAGTGTAGAGA+GGG + chr7.2:19124826-19124845 MS.gene017699:CDS 50.0%
CCCTCTTTCTCCTCCCAAAA+CGG - chr7.2:19124650-19124669 None:intergenic 50.0%
CTACATTGGGAGCTGAGTTG+CGG + chr7.2:19125029-19125048 MS.gene017699:CDS 50.0%
CTCAAGATGCTGAGGAGTTG+AGG + chr7.2:19124978-19124997 MS.gene017699:CDS 50.0%
CTCCACCCCAATTTCTTCAC+AGG - chr7.2:19125067-19125086 None:intergenic 50.0%
CTCCTGTGAAGAAATTGGGG+TGG + chr7.2:19125062-19125081 MS.gene017699:CDS 50.0%
CTGTGAAGAAATTGGGGTGG+AGG + chr7.2:19125065-19125084 MS.gene017699:CDS 50.0%
GGATTAAGCCGAGGATTTCG+CGG - chr7.2:19124088-19124107 None:intergenic 50.0%
TACTAACCGTGGCACGTTGA+AGG + chr7.2:19124928-19124947 MS.gene017699:CDS 50.0%
TAGGAGGACTCAAGATGCTG+AGG + chr7.2:19124970-19124989 MS.gene017699:CDS 50.0%
TCTCTCGGGAGGGATATTTG+CGG + chr7.2:19124721-19124740 MS.gene017699:CDS 50.0%
TCTTTCTCCTCCCAAAACGG+CGG - chr7.2:19124647-19124666 None:intergenic 50.0%
TGAAGAAATTGGGGTGGAGG+AGG + chr7.2:19125068-19125087 MS.gene017699:CDS 50.0%
TGTTAATGCGGGACAGAGTC+AGG + chr7.2:19125123-19125142 MS.gene017699:CDS 50.0%
TTTGAACGATGGCTTCGACG+TGG - chr7.2:19124235-19124254 None:intergenic 50.0%
! GGTAGGTTTCGGATTGGGAT+TGG - chr7.2:19124004-19124023 None:intergenic 50.0%
! TACCTGCAATCGACTCATCC+CGG - chr7.2:19125317-19125336 None:intergenic 50.0%
! TCGGATTGGGATTGGGAGAA+GGG - chr7.2:19123996-19124015 None:intergenic 50.0%
! TGATGGCGTTGGAGTCGTAT+GGG - chr7.2:19124124-19124143 None:intergenic 50.0%
! TTCGGATTGGGATTGGGAGA+AGG - chr7.2:19123997-19124016 None:intergenic 50.0%
!! AAGAGGAGATGGGCTGTTAC+GGG + chr7.2:19125166-19125185 MS.gene017699:CDS 50.0%
!! ATGATATGAGCGTCGAGGAG+AGG - chr7.2:19124212-19124231 None:intergenic 50.0%
!! GAAGAGGAGATGGGCTGTTA+CGG + chr7.2:19125165-19125184 MS.gene017699:CDS 50.0%
!! GCGAGTGTTGGTTTGAACGA+TGG - chr7.2:19124246-19124265 None:intergenic 50.0%
!! GTTGAGGACTTTGATGGCGT+TGG - chr7.2:19124135-19124154 None:intergenic 50.0%
!! TGATTTACCGCCGTTTTGGG+AGG + chr7.2:19124637-19124656 MS.gene017699:CDS 50.0%
!! TTGATGGCGTTGGAGTCGTA+TGG - chr7.2:19124125-19124144 None:intergenic 50.0%
!!! CCGTTTTGGGAGGAGAAAGA+GGG + chr7.2:19124647-19124666 MS.gene017699:CDS 50.0%
!!! CGTTTTGGGAGGAGAAAGAG+GGG + chr7.2:19124648-19124667 MS.gene017699:CDS 50.0%
ACGGGGACGCCTATTCAGAA+TGG + chr7.2:19125184-19125203 MS.gene017699:CDS 55.0%
AGGGATATTTGCGGACGGGA+TGG + chr7.2:19124730-19124749 MS.gene017699:CDS 55.0%
CAATCCGAAACCTACCTCGC+CGG + chr7.2:19124008-19124027 MS.gene017699:CDS 55.0%
CATTACGAAACCGGCGAGGT+AGG - chr7.2:19124021-19124040 None:intergenic 55.0%
CGCGTTCGATGTAACCGACT+TGG - chr7.2:19124169-19124188 None:intergenic 55.0%
GAAACCGGCGAGGTAGGTTT+CGG - chr7.2:19124015-19124034 None:intergenic 55.0%
GAGGTAGGGGTAGTGTAGAG+AGG + chr7.2:19124825-19124844 MS.gene017699:CDS 55.0%
GCAAATATCCCTCCCGAGAG+AGG - chr7.2:19124722-19124741 None:intergenic 55.0%
GCGTGTTTCTTTAACGGCGG+CGG - chr7.2:19124411-19124430 None:intergenic 55.0%
GGCGAGGTAGGTTTCGGATT+GGG - chr7.2:19124009-19124028 None:intergenic 55.0%
GGGATATTTGCGGACGGGAT+GGG + chr7.2:19124731-19124750 MS.gene017699:CDS 55.0%
TAGGGGTAGTGTAGAGAGGG+TGG + chr7.2:19124829-19124848 MS.gene017699:CDS 55.0%
TCGGGAGGGATATTTGCGGA+CGG + chr7.2:19124725-19124744 MS.gene017699:CDS 55.0%
TTGCGGACGGGATGGGATTA+GGG + chr7.2:19124738-19124757 MS.gene017699:CDS 55.0%
TTTGCGGACGGGATGGGATT+AGG + chr7.2:19124737-19124756 MS.gene017699:CDS 55.0%
! AAGGTTCGCGGATTAAGCCG+AGG - chr7.2:19124097-19124116 None:intergenic 55.0%
! CGTGGATGATATGAGCGTCG+AGG - chr7.2:19124217-19124236 None:intergenic 55.0%
! GGGGTGGAGGAGGATTGTTT+TGG + chr7.2:19125078-19125097 MS.gene017699:CDS 55.0%
!! AGAGGAGATGGGCTGTTACG+GGG + chr7.2:19125167-19125186 MS.gene017699:CDS 55.0%
!! CTACCTCGCCGGTTTCGTAA+TGG + chr7.2:19124019-19124038 MS.gene017699:CDS 55.0%
!!! GCCGTTTTGGGAGGAGAAAG+AGG + chr7.2:19124646-19124665 MS.gene017699:CDS 55.0%
ATCACCGGCCGCGAAATCCT+CGG + chr7.2:19124077-19124096 MS.gene017699:CDS 60.0%
CACTACTCCGGCACCATCAC+CGG + chr7.2:19124062-19124081 MS.gene017699:CDS 60.0%
CGGCGAGGTAGGTTTCGGAT+TGG - chr7.2:19124010-19124029 None:intergenic 60.0%
CGGGAGGGATATTTGCGGAC+GGG + chr7.2:19124726-19124745 MS.gene017699:CDS 60.0%
GACCGGGATGAGTCGATTGC+AGG + chr7.2:19125312-19125331 MS.gene017699:CDS 60.0%
GGGAGCTGAGTTGCGGTGTT+CGG + chr7.2:19125036-19125055 MS.gene017699:CDS 60.0%
TAAGCCGAGGATTTCGCGGC+CGG - chr7.2:19124084-19124103 None:intergenic 60.0%
TGGCCATTACGAAACCGGCG+AGG - chr7.2:19124025-19124044 None:intergenic 60.0%
ATCCCTCCCGAGAGAGGCTC+CGG - chr7.2:19124716-19124735 None:intergenic 65.0%
GAGGATTTCGCGGCCGGTGA+TGG - chr7.2:19124078-19124097 None:intergenic 65.0%
GTCGAGGAGAGGAGCGAGAG+CGG - chr7.2:19124201-19124220 None:intergenic 65.0%
GAGGAGAGGAGCGAGAGCGG+CGG - chr7.2:19124198-19124217 None:intergenic 70.0%
! TTCGCGGCCGGTGATGGTGC+CGG - chr7.2:19124072-19124091 None:intergenic 70.0%
GGCCGGAGCCTCTCTCGGGA+GGG + chr7.2:19124711-19124730 MS.gene017699:CDS 75.0%
TGCGCGGCCGGAGCCTCTCT+CGG + chr7.2:19124706-19124725 MS.gene017699:CDS 75.0%
! CGGCCGGAGCCTCTCTCGGG+AGG + chr7.2:19124710-19124729 MS.gene017699:CDS 80.0%
! GCGCGGCCGGAGCCTCTCTC+GGG + chr7.2:19124707-19124726 MS.gene017699:CDS 80.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 19123975 19125384 19123975 ID=MS.gene017699
chr7.2 mRNA 19123975 19125384 19123975 ID=MS.gene017699.t1;Parent=MS.gene017699
chr7.2 exon 19123975 19125384 19123975 ID=MS.gene017699.t1.exon1;Parent=MS.gene017699.t1
chr7.2 CDS 19123975 19125384 19123975 ID=cds.MS.gene017699.t1;Parent=MS.gene017699.t1
Gene Sequence

>MS.gene017699

ATGGAAGACTCACAAGAACCCTTCTCCCAATCCCAATCCGAAACCTACCTCGCCGGTTTCGTAATGGCCAACATCGTCGGCATTAAACACTACTCCGGCACCATCACCGGCCGCGAAATCCTCGGCTTAATCCGCGAACCTTTAAACCCATACGACTCCAACGCCATCAAAGTCCTCAACACTCAAACACTCCAAGTCGGTTACATCGAACGCGACGTCGCCGCCGCTCTCGCTCCTCTCCTCGACGCTCATATCATCCACGTCGAAGCCATCGTTCAAACCAACACTCGCTCTAACAATAACAAATTCCGTATCCCTTGTCAGATTCATATCTTCGCTCATCAATCTGCTTTTGACGCTGTTCATGACGCTTTTAACGGTTCCAATGTTCATTTCATTTCGTATAGTGATCCTTCGTTTACTCTTTCTCATTCCGCCGCCGTTAAAGAAACACGCGCTGACACCGTCAGCGCTGATTCTGTAACAACCGGTAATAATAATAGTAAAAACCTAGATCAAATTTTCAAACTCGTTCGAGAAAACCTAGCTTCCAAAACCCTAGTTTCTGAACCGTTAAACCCTCCTTCTAGCATTATTAAATCAGAGCTTCTGCAGCACCAAAAAGAAGCACTAGGATGGCTTTATCGTCGTGAAAGTGCTGATGATTTACCGCCGTTTTGGGAGGAGAAAGAGGGGAATTTTGTTAATGTGTTGACGAATTATCAAACTAATGCGCGGCCGGAGCCTCTCTCGGGAGGGATATTTGCGGACGGGATGGGATTAGGGAAGACGTTAACGTTGCTATCGTTGATTGGTTATGATAAAATGAAAATGAAAAGTGGGAAGAAAAGAGGTAGGGGTAGTGTAGAGAGGGTGGAGAGTGAGACAAATGGTACATTGATTGTTTGTCCTCCTTCTGTGATTTCGACATGGATAACTCAATTGGAGGAACATACTAACCGTGGCACGTTGAAGGTTTATATGTATTATGGTGATAGGAGGACTCAAGATGCTGAGGAGTTGAGGAAGTATGATATTGTGTTGACTACTTATGCTACATTGGGAGCTGAGTTGCGGTGTTCGGATACTCCTGTGAAGAAATTGGGGTGGAGGAGGATTGTTTTGGATGAGGCGCATACGATCAAGAATGTTAATGCGGGACAGAGTCAGGCTGTGATTGCTTTGAATGCGAAGAGGAGATGGGCTGTTACGGGGACGCCTATTCAGAATGGTTCTTATGACTTGTTTTCTCTTATGGCTTTTTTGCATTTTGAGCCGTTTTCTATTAAGAGTTATTGGCAAAGCTTGGTTCAGCGACCTCTTAATCAAGGAAAACAGACCGGGATGAGTCGATTGCAGGTACAATTCGATAACAATGTATTGATTGTCATTTTTTACTTGCATGTGTAG
Protein sequence

>MS.gene017699.t1

MEDSQEPFSQSQSETYLAGFVMANIVGIKHYSGTITGREILGLIREPLNPYDSNAIKVLNTQTLQVGYIERDVAAALAPLLDAHIIHVEAIVQTNTRSNNNKFRIPCQIHIFAHQSAFDAVHDAFNGSNVHFISYSDPSFTLSHSAAVKETRADTVSADSVTTGNNNSKNLDQIFKLVRENLASKTLVSEPLNPPSSIIKSELLQHQKEALGWLYRRESADDLPPFWEEKEGNFVNVLTNYQTNARPEPLSGGIFADGMGLGKTLTLLSLIGYDKMKMKSGKKRGRGSVERVESETNGTLIVCPPSVISTWITQLEEHTNRGTLKVYMYYGDRRTQDAEELRKYDIVLTTYATLGAELRCSDTPVKKLGWRRIVLDEAHTIKNVNAGQSQAVIALNAKRRWAVTGTPIQNGSYDLFSLMAFLHFEPFSIKSYWQSLVQRPLNQGKQTGMSRLQVQFDNNVLIVIFYLHV