AlfalfaGEDB | MS.gene017720

M. sativa cultivar XinJiangDaYe / MS.gene017720

KEGG: K11644
GO: GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02671.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017720.t1	XP_003613438.2	80.2	116	12	1	4	108	110	225	3.90E-39	170.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017720.t1	Q9XIE1	44.9	118	50	3	4	108	112	227	1.8e-16	86.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017720.t1	G7K6W4	80.2	116	12	1	4	108	110	225	2.8e-39	170.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene017720.t1	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017720.t1	MTR_5g036670	80.172	116	12	1	4	108	110	225	8.77e-54	180
MS.gene017720.t1	MTR_8g062700	52.991	117	43	2	4	108	109	225	1.60e-27	105
MS.gene017720.t1	MTR_8g062700	52.991	117	43	2	4	108	109	225	1.63e-27	105
MS.gene017720.t1	MTR_1g023820	73.438	64	3	1	45	108	64	113	7.98e-25	90.9
MS.gene017720.t1	MTR_6g008970	48.333	120	47	4	4	108	130	249	9.55e-25	97.4
MS.gene017720.t1	MTR_6g008995	50.562	89	31	3	8	84	107	194	3.55e-17	75.9
MS.gene017720.t1	MTR_6g008995	50.588	85	30	2	8	81	50	133	4.00e-17	75.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017720.t1	AT1G59890	44.915	118	50	3	4	108	112	227	2.45e-22	90.5
MS.gene017720.t1	AT1G59890	44.915	118	50	3	4	108	112	227	2.50e-22	90.5
MS.gene017720.t1	AT1G59890	44.915	118	50	3	4	108	112	227	2.50e-22	90.5
MS.gene017720.t1	AT1G59890	44.915	118	50	3	4	108	112	227	2.55e-22	90.5
MS.gene017720.t1	AT1G70060	45.763	118	50	4	4	108	81	197	9.61e-22	89.0
MS.gene017720.t1	AT1G70060	45.763	118	50	4	4	108	81	197	9.61e-22	89.0
MS.gene017720.t1	AT1G24190	52.809	89	31	1	4	81	81	169	4.42e-21	87.0
MS.gene017720.t1	AT1G24190	52.809	89	31	1	4	81	81	169	4.64e-21	87.0
MS.gene017720.t1	AT1G24190	52.809	89	31	1	4	81	81	169	4.64e-21	87.0
MS.gene017720.t1	AT3G01320	42.857	119	52	4	4	108	124	240	2.86e-19	82.0
MS.gene017720.t1	AT3G01320	42.857	119	52	4	4	108	124	240	3.03e-19	81.6
MS.gene017720.t1	AT5G15020	44.340	106	46	2	16	108	129	234	3.03e-19	81.6
MS.gene017720.t1	AT5G15020	44.340	106	46	2	16	108	129	234	3.41e-19	81.6
MS.gene017720.t1	AT1G10450	36.697	109	53	3	4	101	100	203	1.32e-14	68.6
MS.gene017720.t1	AT1G10450	36.697	109	53	3	4	101	100	203	1.32e-14	68.6
MS.gene017720.t1	AT1G10450	38.095	105	49	3	4	97	151	250	1.52e-14	68.6
MS.gene017720.t1	AT1G24200	40.196	102	46	2	11	97	85	186	2.10e-13	63.5
MS.gene017720.t1	AT1G70030	44.156	77	33	1	8	74	81	157	4.11e-11	56.6

Find 24 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCGATGTTCATAGTCATTTC+TGG	0.250122	7.3:-20032017	MS.gene017720:intron
GATGCAGCTGTGGTGGCTTC+TGG	0.261753	7.3:+20031765	None:intergenic
CAAGAAGATCCGGGTGGTTT+TGG	0.291474	7.3:+20031808	None:intergenic
GGAATTCCATGTTTCGTGAT+AGG	0.385326	7.3:-20031725	MS.gene017720:CDS
GAGTTATGTGAACACGATAA+AGG	0.424039	7.3:-20032154	MS.gene017720:intron
ATAAACCATTCAAGAAGATC+CGG	0.456502	7.3:+20031798	None:intergenic
ACCACCCGGATCTTCTTGAA+TGG	0.456644	7.3:-20031803	MS.gene017720:CDS
ACGGCGAGTGCATGTTGAAA+AGG	0.478594	7.3:-20031682	None:intergenic
ATTACTTACCCTTTCGTCTG+AGG	0.548940	7.3:-20032223	MS.gene017720:CDS
TTGCTCATCCTCAGACGAAA+GGG	0.552798	7.3:+20032215	None:intergenic
CATATTGAATACATACAGAA+AGG	0.566288	7.3:-20031993	MS.gene017720:CDS
AACATACCTCTTTGTAGACA+AGG	0.567177	7.3:+20031954	None:intergenic
ACATGCACTCGCCGTAGTGT+TGG	0.571378	7.3:+20031690	None:intergenic
CGAAAGGGTAAGTAATCTCA+TGG	0.573775	7.3:+20032230	None:intergenic
GTTGCTCATCCTCAGACGAA+AGG	0.601293	7.3:+20032214	None:intergenic
TAAACCATTCAAGAAGATCC+GGG	0.611295	7.3:+20031799	None:intergenic
ATGAGCAGATGCAGCTGTGG+TGG	0.620473	7.3:+20031758	None:intergenic
TAACACCCTTGTCTACAAAG+AGG	0.636791	7.3:-20031960	MS.gene017720:intron
AGAGCTCCTATCACGAAACA+TGG	0.647442	7.3:+20031719	None:intergenic
TGCACTCTACCAAAACCACC+CGG	0.648841	7.3:-20031817	MS.gene017720:CDS
AGCATGAGCAGATGCAGCTG+TGG	0.654964	7.3:+20031755	None:intergenic
ACATACCTCTTTGTAGACAA+GGG	0.669671	7.3:+20031955	None:intergenic
GCTCTGCAATGCCAACACTA+CGG	0.674753	7.3:-20031701	MS.gene017720:CDS
ACCATTCAAGAAGATCCGGG+TGG	0.747614	7.3:+20031802	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	CATATTGAATACATACAGAA+AGG	-	chr7.3:20031918-20031937	MS.gene017720:intron	25.0%
!	TAGAATGTTTACGAGTTTAT+TGG	-	chr7.3:20031781-20031800	MS.gene017720:CDS	25.0%
!	TGTTTGCAATGTAGAATTAA+CGG	-	chr7.3:20031822-20031841	MS.gene017720:CDS	25.0%
	ATAAACCATTCAAGAAGATC+CGG	+	chr7.3:20032116-20032135	None:intergenic	30.0%
	AACATACCTCTTTGTAGACA+AGG	+	chr7.3:20031960-20031979	None:intergenic	35.0%
	ACATACCTCTTTGTAGACAA+GGG	+	chr7.3:20031959-20031978	None:intergenic	35.0%
	AGGTATGTTCTGTTATAGTC+AGG	-	chr7.3:20031971-20031990	MS.gene017720:CDS	35.0%
	GAAACCAAGTCTGCATAAAA+AGG	+	chr7.3:20031871-20031890	None:intergenic	35.0%
	GAGTTATGTGAACACGATAA+AGG	-	chr7.3:20031757-20031776	MS.gene017720:CDS	35.0%
	GTCAGGAAAATTTGTGATGT+TGG	-	chr7.3:20031988-20032007	MS.gene017720:CDS	35.0%
	TAAACCATTCAAGAAGATCC+GGG	+	chr7.3:20032115-20032134	None:intergenic	35.0%
!	TTTATGCAGACTTGGTTTCA+GGG	-	chr7.3:20031872-20031891	MS.gene017720:intron	35.0%
!!	TTTTATGCAGACTTGGTTTC+AGG	-	chr7.3:20031871-20031890	MS.gene017720:intron	35.0%
!!!	GGTTTCAGTTTTGAGCTTAA+TGG	-	chr7.3:20031843-20031862	MS.gene017720:intron	35.0%
	AATCGTAATTGCACTTCTCC+AGG	-	chr7.3:20032067-20032086	MS.gene017720:intron	40.0%
	ATTACTTACCCTTTCGTCTG+AGG	-	chr7.3:20031688-20031707	MS.gene017720:CDS	40.0%
	CACAGCAATATGTCAAGTAC+TGG	+	chr7.3:20032036-20032055	None:intergenic	40.0%
	CCTCGTCTAAAAAAGAAGGT+TGG	-	chr7.3:20031719-20031738	MS.gene017720:CDS	40.0%
	GCAACCTCGTCTAAAAAAGA+AGG	-	chr7.3:20031715-20031734	MS.gene017720:CDS	40.0%
	GCGATGTTCATAGTCATTTC+TGG	-	chr7.3:20031894-20031913	MS.gene017720:intron	40.0%
	GGAATTCCATGTTTCGTGAT+AGG	-	chr7.3:20032186-20032205	MS.gene017720:CDS	40.0%
	TAACACCCTTGTCTACAAAG+AGG	-	chr7.3:20031951-20031970	MS.gene017720:intron	40.0%
!	GGTTCCTTTTTATGCAGACT+TGG	-	chr7.3:20031864-20031883	MS.gene017720:intron	40.0%
!!	AGCAATATGTCAAGTACTGG+AGG	+	chr7.3:20032033-20032052	None:intergenic	40.0%
!!!	CCAACCTTCTTTTTTAGACG+AGG	+	chr7.3:20031722-20031741	None:intergenic	40.0%
	AGAGCTCCTATCACGAAACA+TGG	+	chr7.3:20032195-20032214	None:intergenic	45.0%
	TTGCTCATCCTCAGACGAAA+GGG	+	chr7.3:20031699-20031718	None:intergenic	45.0%
!	CATCTGCTCATGCTTTTGCT+CGG	-	chr7.3:20032165-20032184	MS.gene017720:CDS	45.0%
	ACCACCCGGATCTTCTTGAA+TGG	-	chr7.3:20032108-20032127	MS.gene017720:intron	50.0%
	ACCATTCAAGAAGATCCGGG+TGG	+	chr7.3:20032112-20032131	None:intergenic	50.0%
	GCTCTGCAATGCCAACACTA+CGG	-	chr7.3:20032210-20032229	MS.gene017720:CDS	50.0%
	GTTGCTCATCCTCAGACGAA+AGG	+	chr7.3:20031700-20031719	None:intergenic	50.0%
	TGCACTCTACCAAAACCACC+CGG	-	chr7.3:20032094-20032113	MS.gene017720:intron	50.0%
	TTTGGTAGAGTGCAGCAACC+TGG	+	chr7.3:20032088-20032107	None:intergenic	50.0%
!	CAAGAAGATCCGGGTGGTTT+TGG	+	chr7.3:20032106-20032125	None:intergenic	50.0%
	ACATGCACTCGCCGTAGTGT+TGG	+	chr7.3:20032224-20032243	None:intergenic	55.0%
	AGCATGAGCAGATGCAGCTG+TGG	+	chr7.3:20032159-20032178	None:intergenic	55.0%
	ATGAGCAGATGCAGCTGTGG+TGG	+	chr7.3:20032156-20032175	None:intergenic	55.0%
	GATGCAGCTGTGGTGGCTTC+TGG	+	chr7.3:20032149-20032168	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	20031683	20032250	20031683	ID=MS.gene017720
chr7.3	mRNA	20031683	20032250	20031683	ID=MS.gene017720.t1;Parent=MS.gene017720
chr7.3	exon	20032155	20032250	20032155	ID=MS.gene017720.t1.exon1;Parent=MS.gene017720.t1
chr7.3	CDS	20032155	20032250	20032155	ID=cds.MS.gene017720.t1;Parent=MS.gene017720.t1
chr7.3	exon	20031961	20032026	20031961	ID=MS.gene017720.t1.exon2;Parent=MS.gene017720.t1
chr7.3	CDS	20031961	20032026	20031961	ID=cds.MS.gene017720.t1;Parent=MS.gene017720.t1
chr7.3	exon	20031683	20031844	20031683	ID=MS.gene017720.t1.exon3;Parent=MS.gene017720.t1
chr7.3	CDS	20031683	20031844	20031683	ID=cds.MS.gene017720.t1;Parent=MS.gene017720.t1

Gene Sequence

>MS.gene017720

ATGAGATTACTTACCCTTTCGTCTGAGGATGAGCAACCTCGTCTAAAAAAGAAGGTTGGATTTTATGAAGCTATGAGTTATGTGAACACGATAAAGGTTAGAATGTTTACGAGTTTATTGGATTCTCTTCATGCAGATTTGTTTGCAATGTAGAATTAACGGTTTCAGTTTTGAGCTTAATGGTTCCTTTTTATGCAGACTTGGTTTCAGGGCGATGTTCATAGTCATTTCTGGACATATTGAATACATACAGAAAGGAAAGTAAGTCTAACACCCTTGTCTACAAAGAGGTATGTTCTGTTATAGTCAGGAAAATTTGTGATGTTGGTTTTTTCATTTTTTTATTTCCTCCAGTACTTGACATATTGCTGTGATACACATTTTAATCGTAATTGCACTTCTCCAGGTTGCTGCACTCTACCAAAACCACCCGGATCTTCTTGAATGGTTTATTCGTTTTCTTCCAGAAGCCACCACAGCTGCATCTGCTCATGCTTTTGCTCGGAATTCCATGTTTCGTGATAGGAGCTCTGCAATGCCAACACTACGGCGAGTGCATGTTGAAAAG

Protein sequence

>MS.gene017720.t1

MRLLTLSSEDEQPRLKKKVGFYEAMSYVNTIKSFLDILNTYRKESKSNTLVYKEVAALYQNHPDLLEWFIRFLPEATTAASAHAFARNSMFRDRSSAMPTLRRVHVEK