Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017793.t1 | XP_003625051.2 | 95 | 220 | 9 | 2 | 1 | 219 | 1 | 219 | 2.00E-106 | 395.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017793.t1 | Q9M1K1 | 43.1 | 225 | 109 | 7 | 7 | 219 | 6 | 223 | 1.1e-33 | 144.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017793.t1 | G7KRQ0 | 95.0 | 220 | 9 | 2 | 1 | 219 | 1 | 219 | 1.4e-106 | 395.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene017793.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017793.t1 | MTR_7g090410 | 95.000 | 220 | 9 | 2 | 1 | 219 | 1 | 219 | 1.76e-124 | 353 |
| MS.gene017793.t1 | MTR_1g048720 | 58.296 | 223 | 84 | 6 | 1 | 219 | 1 | 218 | 1.50e-75 | 227 |
| MS.gene017793.t1 | MTR_1g048720 | 58.515 | 229 | 74 | 6 | 1 | 219 | 1 | 218 | 2.57e-75 | 228 |
| MS.gene017793.t1 | MTR_1g048720 | 57.848 | 223 | 84 | 7 | 1 | 219 | 1 | 217 | 4.22e-73 | 221 |
| MS.gene017793.t1 | MTR_1g048720 | 58.079 | 229 | 74 | 7 | 1 | 219 | 1 | 217 | 7.38e-73 | 221 |
| MS.gene017793.t1 | MTR_1g048750 | 55.895 | 229 | 72 | 5 | 1 | 219 | 1 | 210 | 6.10e-72 | 220 |
| MS.gene017793.t1 | MTR_1g048710 | 58.296 | 223 | 75 | 8 | 1 | 219 | 1 | 209 | 1.39e-65 | 202 |
| MS.gene017793.t1 | MTR_0007s0330 | 34.211 | 152 | 91 | 4 | 67 | 216 | 6 | 150 | 7.55e-12 | 61.6 |
| MS.gene017793.t1 | MTR_0007s0330 | 34.211 | 152 | 91 | 4 | 67 | 216 | 18 | 162 | 1.08e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017793.t1 | AT3G56970 | 44.156 | 231 | 109 | 9 | 1 | 219 | 1 | 223 | 3.42e-49 | 161 |
| MS.gene017793.t1 | AT2G41240 | 41.410 | 227 | 113 | 6 | 1 | 219 | 1 | 215 | 3.35e-41 | 140 |
| MS.gene017793.t1 | AT3G56980 | 42.060 | 233 | 116 | 5 | 1 | 219 | 1 | 228 | 7.15e-40 | 137 |
| MS.gene017793.t1 | AT2G41240 | 41.410 | 227 | 112 | 7 | 1 | 219 | 1 | 214 | 8.93e-39 | 134 |
| MS.gene017793.t1 | AT5G04150 | 43.713 | 167 | 85 | 3 | 57 | 219 | 76 | 237 | 1.18e-36 | 129 |
| MS.gene017793.t1 | AT5G04150 | 45.181 | 166 | 84 | 3 | 57 | 219 | 76 | 237 | 2.66e-36 | 128 |
Find 35 sgRNAs with CRISPR-Local
Find 76 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCTCTTTATGACTAATTA+AGG | 0.198069 | 7.3:+18700067 | None:intergenic |
| AGCTTCTTAGCCATAAGTTT+AGG | 0.275147 | 7.3:+18699957 | None:intergenic |
| TCACTTCGTTCACTTCTTCC+TGG | 0.286326 | 7.3:-18699879 | MS.gene017793:CDS |
| GCTTCTTAGCCATAAGTTTA+GGG | 0.291086 | 7.3:+18699958 | None:intergenic |
| TCTTCTTCTTCTAAAACCTT+TGG | 0.334379 | 7.3:-18698788 | MS.gene017793:CDS |
| AAGACACGTGAAATTGTTAC+AGG | 0.334795 | 7.3:+18699079 | None:intergenic |
| GCCACAATCCCCTAAACTTA+TGG | 0.343588 | 7.3:-18699967 | MS.gene017793:CDS |
| TCAATCTCAACATGTTCCTT+TGG | 0.352411 | 7.3:+18699993 | None:intergenic |
| TTGATGTGTTTAGAAAATAA+TGG | 0.374175 | 7.3:-18698827 | MS.gene017793:CDS |
| AAACATGGGATGGCTCTTAG+AGG | 0.417919 | 7.3:-18700105 | MS.gene017793:CDS |
| GGTGAAGAGAATTGATGATA+AGG | 0.444850 | 7.3:+18700014 | None:intergenic |
| GGGATGGCTCTTAGAGGAGT+TGG | 0.478368 | 7.3:-18700099 | MS.gene017793:CDS |
| CTCAGATAGAGGAATCTTGT+AGG | 0.481665 | 7.3:+18698853 | None:intergenic |
| TCTTCTTCTAAAACCTTTGG+AGG | 0.482465 | 7.3:-18698785 | MS.gene017793:CDS |
| CACATCAAGATCTCAGATAG+AGG | 0.501569 | 7.3:+18698842 | None:intergenic |
| GCCATAAGTTTAGGGGATTG+TGG | 0.507590 | 7.3:+18699966 | None:intergenic |
| CTTCTAAAACCTTTGGAGGG+AGG | 0.546758 | 7.3:-18698781 | MS.gene017793:CDS |
| ACGTGTCTTAAAATACATAC+CGG | 0.551566 | 7.3:-18699065 | MS.gene017793:CDS |
| TCTTCCTGGTGAAGATCAAA+CGG | 0.553586 | 7.3:-18699865 | MS.gene017793:intron |
| ACAAAAGCAGAATTGTAACT+TGG | 0.558897 | 7.3:+18698935 | None:intergenic |
| CTTCTTAGCCATAAGTTTAG+GGG | 0.566289 | 7.3:+18699959 | None:intergenic |
| CTTCTTCTAAAACCTTTGGA+GGG | 0.566421 | 7.3:-18698784 | MS.gene017793:CDS |
| ATAGAAGAGCCTCCCTCCAA+AGG | 0.569392 | 7.3:+18698772 | None:intergenic |
| TCATCAATTCTCTTCACCAA+AGG | 0.579398 | 7.3:-18700009 | MS.gene017793:CDS |
| GAGTTCTCATACTCAAACAT+GGG | 0.591739 | 7.3:-18700119 | MS.gene017793:CDS |
| TCTCATACTCAAACATGGGA+TGG | 0.596383 | 7.3:-18700115 | MS.gene017793:CDS |
| TGAGTTCTCATACTCAAACA+TGG | 0.598383 | 7.3:-18700120 | MS.gene017793:CDS |
| GATTTACAAAAGCAAGTGCA+AGG | 0.608962 | 7.3:-18699043 | MS.gene017793:CDS |
| TTTGAGTATGAGAACTCAGG+TGG | 0.609689 | 7.3:+18700125 | None:intergenic |
| ATGTTTGAGTATGAGAACTC+AGG | 0.624831 | 7.3:+18700122 | None:intergenic |
| AGACACGTGAAATTGTTACA+GGG | 0.628066 | 7.3:+18699080 | None:intergenic |
| ATTACCGTTTGATCTTCACC+AGG | 0.645561 | 7.3:+18699861 | None:intergenic |
| CAGTTAACAAAGAATCGCAA+AGG | 0.655004 | 7.3:-18698967 | MS.gene017793:CDS |
| GATCACGTTCACTAGCATTG+TGG | 0.694395 | 7.3:+18699931 | None:intergenic |
| TTGAGTATGAGAACTCAGGT+GGG | 0.707297 | 7.3:+18700126 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TGGAATAAAATATATATTAA+TGG | + | chr7.3:18699611-18699630 | None:intergenic | 10.0% |
| !!! | AATTTTTTTTTCAAATAGAT+TGG | + | chr7.3:18699242-18699261 | None:intergenic | 10.0% |
| !! | CATACCAAATTAAATAAAAT+TGG | + | chr7.3:18698921-18698940 | None:intergenic | 15.0% |
| !!! | CAATTTTATTTAATTTGGTA+TGG | - | chr7.3:18698919-18698938 | MS.gene017793:CDS | 15.0% |
| !! | CCAAATTAAATAAAATTGGA+AGG | + | chr7.3:18698917-18698936 | None:intergenic | 20.0% |
| !! | TTGATGTGTTTAGAAAATAA+TGG | - | chr7.3:18699912-18699931 | MS.gene017793:CDS | 20.0% |
| !!! | AAGCTATTTTTTAAAATGAC+AGG | - | chr7.3:18698996-18699015 | MS.gene017793:CDS | 20.0% |
| !!! | CCTTCCAATTTTATTTAATT+TGG | - | chr7.3:18698914-18698933 | MS.gene017793:CDS | 20.0% |
| !!! | TAACCTACTTTAAGAAAAAA+GGG | - | chr7.3:18699404-18699423 | MS.gene017793:intron | 20.0% |
| !!! | TAGATTGGTTAGTTTTTTTT+TGG | + | chr7.3:18699227-18699246 | None:intergenic | 20.0% |
| ! | ACCCTAATTGTTTACTATTA+AGG | - | chr7.3:18700129-18700148 | MS.gene017793:CDS | 25.0% |
| ! | ATAGCTAATCGTTCTTTAAA+AGG | - | chr7.3:18699359-18699378 | MS.gene017793:intron | 25.0% |
| ! | ATGTGCAAAAAAATTAGTGA+TGG | - | chr7.3:18699311-18699330 | MS.gene017793:intron | 25.0% |
| ! | CCCTAATTGTTTACTATTAA+GGG | - | chr7.3:18700130-18700149 | MS.gene017793:CDS | 25.0% |
| ! | CCCTTAATAGTAAACAATTA+GGG | + | chr7.3:18700133-18700152 | None:intergenic | 25.0% |
| ! | CTTCTCTTTATGACTAATTA+AGG | + | chr7.3:18698675-18698694 | None:intergenic | 25.0% |
| ! | TCCCTTAATAGTAAACAATT+AGG | + | chr7.3:18700134-18700153 | None:intergenic | 25.0% |
| !! | GTAACCTACTTTAAGAAAAA+AGG | - | chr7.3:18699403-18699422 | MS.gene017793:intron | 25.0% |
| !!! | TTTAGCTTTATATAACTCCA+TGG | - | chr7.3:18699025-18699044 | MS.gene017793:CDS | 25.0% |
| ACAAAAGCAGAATTGTAACT+TGG | + | chr7.3:18699807-18699826 | None:intergenic | 30.0% | |
| ACGTGTCTTAAAATACATAC+CGG | - | chr7.3:18699674-18699693 | MS.gene017793:intron | 30.0% | |
| CCAATAATTGATTAATCCAG+AGG | + | chr7.3:18699181-18699200 | None:intergenic | 30.0% | |
| CCTCTGGATTAATCAATTAT+TGG | - | chr7.3:18699178-18699197 | MS.gene017793:intron | 30.0% | |
| CTAGAGATAAGCATAATCAT+AGG | + | chr7.3:18699076-18699095 | None:intergenic | 30.0% | |
| CTATGATTATGCTTATCTCT+AGG | - | chr7.3:18699074-18699093 | MS.gene017793:CDS | 30.0% | |
| TGACGTATTCATGATATGAA+TGG | - | chr7.3:18699534-18699553 | MS.gene017793:intron | 30.0% | |
| ! | CTGTTTGTTTCGTAATGTTT+TGG | - | chr7.3:18699458-18699477 | MS.gene017793:intron | 30.0% |
| !! | AAGAGTTTTGATCTGACTAT+TGG | - | chr7.3:18699125-18699144 | MS.gene017793:intron | 30.0% |
| !! | CTTCTATAACTTGCATTTTC+AGG | - | chr7.3:18699983-18700002 | MS.gene017793:CDS | 30.0% |
| !! | TCTTCTTCTTCTAAAACCTT+TGG | - | chr7.3:18699951-18699970 | MS.gene017793:CDS | 30.0% |
| !!! | ACACCCTTTTTTCTTAAAGT+AGG | + | chr7.3:18699410-18699429 | None:intergenic | 30.0% |
| !!! | TTTTTTCTGCAACATCAACA+TGG | + | chr7.3:18699631-18699650 | None:intergenic | 30.0% |
| AAGACACGTGAAATTGTTAC+AGG | + | chr7.3:18699663-18699682 | None:intergenic | 35.0% | |
| AGACACGTGAAATTGTTACA+GGG | + | chr7.3:18699662-18699681 | None:intergenic | 35.0% | |
| AGCTTCTTAGCCATAAGTTT+AGG | + | chr7.3:18698785-18698804 | None:intergenic | 35.0% | |
| ATGTTTGAGTATGAGAACTC+AGG | + | chr7.3:18698620-18698639 | None:intergenic | 35.0% | |
| CAGTTAACAAAGAATCGCAA+AGG | - | chr7.3:18699772-18699791 | MS.gene017793:intron | 35.0% | |
| CTTCTTAGCCATAAGTTTAG+GGG | + | chr7.3:18698783-18698802 | None:intergenic | 35.0% | |
| GAGTTCTCATACTCAAACAT+GGG | - | chr7.3:18698620-18698639 | MS.gene017793:intron | 35.0% | |
| GATTTACAAAAGCAAGTGCA+AGG | - | chr7.3:18699696-18699715 | MS.gene017793:intron | 35.0% | |
| GCTTCTTAGCCATAAGTTTA+GGG | + | chr7.3:18698784-18698803 | None:intergenic | 35.0% | |
| GCTTTATATAACTCCATGGT+TGG | - | chr7.3:18699029-18699048 | MS.gene017793:CDS | 35.0% | |
| TCAATCTCAACATGTTCCTT+TGG | + | chr7.3:18698749-18698768 | None:intergenic | 35.0% | |
| TCATCAATTCTCTTCACCAA+AGG | - | chr7.3:18698730-18698749 | MS.gene017793:intron | 35.0% | |
| TGAGTTCTCATACTCAAACA+TGG | - | chr7.3:18698619-18698638 | MS.gene017793:intron | 35.0% | |
| ! | CTTTTGTAGTTTCGACAAGT+AGG | - | chr7.3:18699820-18699839 | MS.gene017793:intron | 35.0% |
| ! | GGTGAAGAGAATTGATGATA+AGG | + | chr7.3:18698728-18698747 | None:intergenic | 35.0% |
| ! | TAGGTCTGATGATGAAAGTT+TGG | - | chr7.3:18699093-18699112 | MS.gene017793:CDS | 35.0% |
| !! | CTTCTTCTAAAACCTTTGGA+GGG | - | chr7.3:18699955-18699974 | MS.gene017793:CDS | 35.0% |
| !! | TCTTCTTCTAAAACCTTTGG+AGG | - | chr7.3:18699954-18699973 | MS.gene017793:CDS | 35.0% |
| !! | TGCACTTGCTTTTGTAAATC+CGG | + | chr7.3:18699696-18699715 | None:intergenic | 35.0% |
| ATTACCGTTTGATCTTCACC+AGG | + | chr7.3:18698881-18698900 | None:intergenic | 40.0% | |
| CACATCAAGATCTCAGATAG+AGG | + | chr7.3:18699900-18699919 | None:intergenic | 40.0% | |
| CTCAGATAGAGGAATCTTGT+AGG | + | chr7.3:18699889-18699908 | None:intergenic | 40.0% | |
| TCTCATACTCAAACATGGGA+TGG | - | chr7.3:18698624-18698643 | MS.gene017793:intron | 40.0% | |
| TCTTCCTGGTGAAGATCAAA+CGG | - | chr7.3:18698874-18698893 | MS.gene017793:CDS | 40.0% | |
| TTGAGTATGAGAACTCAGGT+GGG | + | chr7.3:18698616-18698635 | None:intergenic | 40.0% | |
| TTTCATCATCAGACCAACCA+TGG | + | chr7.3:18699045-18699064 | None:intergenic | 40.0% | |
| TTTGAGTATGAGAACTCAGG+TGG | + | chr7.3:18698617-18698636 | None:intergenic | 40.0% | |
| AAACATGGGATGGCTCTTAG+AGG | - | chr7.3:18698634-18698653 | MS.gene017793:intron | 45.0% | |
| GATCACGTTCACTAGCATTG+TGG | + | chr7.3:18698811-18698830 | None:intergenic | 45.0% | |
| GCCACAATCCCCTAAACTTA+TGG | - | chr7.3:18698772-18698791 | MS.gene017793:CDS | 45.0% | |
| GCCATAAGTTTAGGGGATTG+TGG | + | chr7.3:18698776-18698795 | None:intergenic | 45.0% | |
| TCACTTCGTTCACTTCTTCC+TGG | - | chr7.3:18698860-18698879 | MS.gene017793:CDS | 45.0% | |
| !! | CTTCTAAAACCTTTGGAGGG+AGG | - | chr7.3:18699958-18699977 | MS.gene017793:CDS | 45.0% |
| ATAGAAGAGCCTCCCTCCAA+AGG | + | chr7.3:18699970-18699989 | None:intergenic | 50.0% | |
| CTATTGGACCCTCGCTAGAG+TGG | - | chr7.3:18699141-18699160 | MS.gene017793:intron | 55.0% | |
| GGGATGGCTCTTAGAGGAGT+TGG | - | chr7.3:18698640-18698659 | MS.gene017793:intron | 55.0% | |
| ACCCTCGCTAGAGTGGATGG+TGG | - | chr7.3:18699148-18699167 | MS.gene017793:intron | 60.0% | |
| CCCACCATCCACTCTAGCGA+GGG | + | chr7.3:18699152-18699171 | None:intergenic | 60.0% | |
| CCCTCGCTAGAGTGGATGGT+GGG | - | chr7.3:18699149-18699168 | MS.gene017793:intron | 60.0% | |
| CCTCGCTAGAGTGGATGGTG+GGG | - | chr7.3:18699150-18699169 | MS.gene017793:intron | 60.0% | |
| TGGACCCTCGCTAGAGTGGA+TGG | - | chr7.3:18699145-18699164 | MS.gene017793:intron | 60.0% | |
| !! | GCTAGAGTGGATGGTGGGGT+CGG | - | chr7.3:18699154-18699173 | MS.gene017793:intron | 60.0% |
| CCCCACCATCCACTCTAGCG+AGG | + | chr7.3:18699153-18699172 | None:intergenic | 65.0% | |
| !! | GGATGGTGGGGTCGGTCCTC+TGG | - | chr7.3:18699162-18699181 | MS.gene017793:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 18698599 | 18700162 | 18698599 | ID=MS.gene017793 |
| chr7.3 | mRNA | 18698599 | 18700162 | 18698599 | ID=MS.gene017793.t1;Parent=MS.gene017793 |
| chr7.3 | exon | 18699866 | 18700162 | 18699866 | ID=MS.gene017793.t1.exon1;Parent=MS.gene017793.t1 |
| chr7.3 | CDS | 18699866 | 18700162 | 18699866 | ID=cds.MS.gene017793.t1;Parent=MS.gene017793.t1 |
| chr7.3 | exon | 18698757 | 18699116 | 18698757 | ID=MS.gene017793.t1.exon2;Parent=MS.gene017793.t1 |
| chr7.3 | CDS | 18698757 | 18699116 | 18698757 | ID=cds.MS.gene017793.t1;Parent=MS.gene017793.t1 |
| chr7.3 | exon | 18698599 | 18698610 | 18698599 | ID=MS.gene017793.t1.exon3;Parent=MS.gene017793.t1 |
| chr7.3 | CDS | 18698599 | 18698610 | 18698599 | ID=cds.MS.gene017793.t1;Parent=MS.gene017793.t1 |
| Gene Sequence |
| Protein sequence |
