AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene017974

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017974.t1 MTR_7g093030 95.568 361 8 2 1 359 33 387 0.0 697
MS.gene017974.t1 MTR_7g093030 96.269 268 4 1 1 268 33 294 0.0 528
MS.gene017974.t1 MTR_1g053835 61.345 357 112 8 1 345 80 422 1.11e-136 396
MS.gene017974.t1 MTR_7g098250 51.111 180 87 1 175 353 251 430 1.95e-58 197
MS.gene017974.t1 MTR_1g080330 57.042 142 60 1 177 317 166 307 3.99e-51 175
MS.gene017974.t1 MTR_7g115530 53.521 142 65 1 185 325 217 358 1.90e-47 166
MS.gene017974.t1 MTR_7g088070 50.725 138 68 0 184 321 48 185 1.90e-47 162
MS.gene017974.t1 MTR_2g027860 51.724 145 67 2 186 327 49 193 7.36e-46 159
MS.gene017974.t1 MTR_7g088070 49.296 142 68 1 184 321 48 189 3.15e-45 156
MS.gene017974.t1 MTR_1g080330 57.265 117 49 1 177 292 166 282 5.74e-41 145
MS.gene017974.t1 MTR_7g115530 56.881 109 46 1 185 292 217 325 4.80e-38 139
MS.gene017974.t1 MTR_6g032990 45.395 152 64 3 185 318 41 191 9.12e-37 137
MS.gene017974.t1 MTR_2g086450 45.087 173 69 6 169 317 27 197 6.50e-36 134
MS.gene017974.t1 MTR_1g053830 61.053 95 37 0 169 263 1 95 2.03e-35 125
MS.gene017974.t1 MTR_7g068600 47.183 142 70 3 173 310 1 141 2.53e-35 129
MS.gene017974.t1 MTR_1g053800 50.350 143 70 1 175 316 165 307 5.38e-35 130
MS.gene017974.t1 MTR_5g041350 35.032 157 89 2 185 328 14 170 2.18e-30 119
MS.gene017974.t1 MTR_2g027800 36.723 177 95 5 186 358 39 202 2.61e-30 117
MS.gene017974.t1 MTR_5g027440 41.096 146 73 3 186 318 25 170 9.79e-30 115
MS.gene017974.t1 MTR_6g444980 40.000 160 84 5 179 328 26 183 7.35e-29 115
MS.gene017974.t1 MTR_4g081710 40.000 145 75 4 185 317 16 160 1.05e-28 114
MS.gene017974.t1 MTR_2g027800 34.946 186 100 4 186 358 39 216 3.74e-28 112
MS.gene017974.t1 MTR_7g115530 56.471 85 36 1 185 268 217 301 7.70e-28 111
MS.gene017974.t1 MTR_6g444980 40.000 155 83 4 179 325 26 178 7.87e-28 112
MS.gene017974.t1 MTR_7g115530 66.667 66 22 0 185 250 217 282 6.02e-26 107
MS.gene017974.t1 MTR_1g090670 36.196 163 83 4 186 333 44 200 9.93e-26 105
MS.gene017974.t1 MTR_7g093010 43.357 143 77 2 177 317 204 344 4.93e-25 104
MS.gene017974.t1 MTR_7g115530 67.925 53 17 0 185 237 217 269 1.27e-19 87.8
MS.gene017974.t1 MTR_7g115530 67.308 52 17 0 185 236 217 268 9.50e-19 85.9
MS.gene017974.t1 MTR_3g064840 48.837 86 41 2 188 271 276 360 7.54e-18 84.7
MS.gene017974.t1 MTR_7g069660 49.367 79 39 1 182 260 67 144 9.90e-18 83.6
MS.gene017974.t1 MTR_8g066310 54.412 68 29 1 188 253 175 242 1.56e-17 83.2
MS.gene017974.t1 MTR_2g023580 59.016 61 25 0 182 242 51 111 2.23e-17 80.9
MS.gene017974.t1 MTR_2g084230 38.125 160 85 5 108 261 26 177 2.30e-17 82.0
MS.gene017974.t1 MTR_2g084230 38.125 160 85 5 108 261 26 177 3.30e-17 82.4
MS.gene017974.t1 MTR_8g086410 56.452 62 27 0 182 243 107 168 6.34e-17 82.0
MS.gene017974.t1 MTR_8g086410 56.452 62 27 0 182 243 105 166 6.40e-17 82.0
MS.gene017974.t1 MTR_6g071625 60.000 55 22 0 188 242 204 258 4.39e-16 79.0
MS.gene017974.t1 MTR_1g112370 50.000 78 35 2 169 242 1 78 4.46e-16 78.6
MS.gene017974.t1 MTR_3g450310 55.932 59 26 0 184 242 17 75 6.66e-16 76.3
MS.gene017974.t1 MTR_5g029470 60.000 55 22 0 188 242 221 275 6.95e-16 78.6
MS.gene017974.t1 MTR_1g021520 53.968 63 29 0 188 250 128 190 9.80e-16 77.0
MS.gene017974.t1 MTR_0223s0040 55.738 61 27 0 182 242 14 74 1.19e-15 76.6
MS.gene017974.t1 MTR_2g016220 54.839 62 28 0 182 243 85 146 1.20e-15 77.8
MS.gene017974.t1 MTR_2g016220 54.839 62 28 0 182 243 85 146 1.21e-15 77.8
MS.gene017974.t1 MTR_8g086410 55.932 59 26 0 182 240 107 165 2.60e-15 77.0
MS.gene017974.t1 MTR_2g016220 51.515 66 28 1 182 243 85 150 1.04e-13 72.0
MS.gene017974.t1 MTR_4g021790 51.786 56 26 1 184 239 166 220 6.82e-12 65.5
MS.gene017974.t1 MTR_4g113140 49.231 65 33 0 175 239 224 288 8.94e-12 66.2
MS.gene017974.t1 MTR_4g021855 50.000 62 30 1 178 239 160 220 9.32e-12 65.5
MS.gene017974.t1 MTR_1g093080 37.755 98 46 1 170 252 180 277 2.78e-11 64.7
MS.gene017974.t1 MTR_5g017980 53.571 56 26 0 184 239 191 246 8.00e-11 62.8
MS.gene017974.t1 MTR_4g097835 64.444 45 15 1 251 295 8 51 8.39e-11 57.4
MS.gene017974.t1 MTR_4g086835 50.877 57 28 0 183 239 271 327 9.46e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene017974.t1 AT5G06800 57.727 220 86 6 109 322 110 328 6.22e-69 221
MS.gene017974.t1 AT5G06800 56.444 225 86 7 109 322 110 333 2.55e-66 214
MS.gene017974.t1 AT5G29000 53.947 152 66 1 173 320 175 326 2.29e-52 178
MS.gene017974.t1 AT5G29000 53.947 152 66 1 173 320 175 326 2.29e-52 178
MS.gene017974.t1 AT5G29000 53.947 152 66 1 173 320 218 369 4.07e-52 178
MS.gene017974.t1 AT3G04450 45.055 182 89 3 143 320 162 336 9.61e-50 172
MS.gene017974.t1 AT3G04450 45.055 182 89 3 143 320 162 336 9.61e-50 172
MS.gene017974.t1 AT3G04450 45.055 182 89 3 143 320 202 376 2.34e-49 172
MS.gene017974.t1 AT4G28610 54.861 144 63 1 173 314 212 355 7.37e-49 169
MS.gene017974.t1 AT2G01060 50.331 151 73 2 184 332 13 163 2.54e-47 162
MS.gene017974.t1 AT3G13040 48.214 168 78 4 159 317 211 378 1.28e-44 159
MS.gene017974.t1 AT3G13040 48.214 168 78 4 159 317 211 378 1.28e-44 159
MS.gene017974.t1 AT3G13040 48.214 168 78 4 159 317 211 378 1.28e-44 159
MS.gene017974.t1 AT2G20400 53.077 130 60 1 186 314 231 360 9.40e-42 150
MS.gene017974.t1 AT2G20400 53.435 131 59 2 186 314 231 361 3.23e-40 146
MS.gene017974.t1 AT3G04030 45.963 161 69 3 175 317 32 192 7.19e-38 140
MS.gene017974.t1 AT5G06800 56.081 148 58 6 109 250 110 256 2.77e-37 135
MS.gene017974.t1 AT5G18240 44.720 161 71 3 176 318 33 193 1.16e-36 137
MS.gene017974.t1 AT5G18240 44.720 161 71 3 176 318 33 193 1.16e-36 137
MS.gene017974.t1 AT3G04030 44.720 161 70 4 175 317 32 191 6.69e-35 132
MS.gene017974.t1 AT3G24120 41.667 156 71 2 186 321 41 196 2.12e-34 128
MS.gene017974.t1 AT1G69580 42.581 155 63 3 185 317 33 183 3.66e-34 129
MS.gene017974.t1 AT5G18240 44.099 161 70 4 176 318 33 191 1.09e-33 129
MS.gene017974.t1 AT1G69580 41.667 156 64 3 185 317 33 184 4.28e-33 126
MS.gene017974.t1 AT3G04030 43.478 161 67 4 175 317 32 186 1.19e-32 125
MS.gene017974.t1 AT1G79430 43.243 148 76 4 179 319 25 171 2.33e-32 124
MS.gene017974.t1 AT3G24120 40.881 159 71 3 186 321 41 199 2.37e-32 123
MS.gene017974.t1 AT4G13640 34.737 190 108 2 186 359 37 226 2.54e-32 123
MS.gene017974.t1 AT5G45580 40.606 165 67 5 186 324 7 166 3.74e-32 122
MS.gene017974.t1 AT5G45580 40.606 165 67 5 186 324 23 182 3.98e-32 122
MS.gene017974.t1 AT5G18240 42.236 161 69 4 176 318 33 187 2.34e-31 122
MS.gene017974.t1 AT5G18240 42.236 161 69 4 176 318 33 187 2.34e-31 122
MS.gene017974.t1 AT4G13640 34.184 196 104 4 186 359 37 229 2.40e-30 117
MS.gene017974.t1 AT2G01060 49.123 114 56 2 221 332 1 114 1.44e-28 111
MS.gene017974.t1 AT5G29000 57.955 88 35 1 173 258 218 305 2.54e-28 112
MS.gene017974.t1 AT3G04450 46.154 104 49 2 143 246 202 298 3.10e-26 107
MS.gene017974.t1 AT3G12730 38.562 153 81 5 177 317 12 163 3.64e-25 102
MS.gene017974.t1 AT2G42660 55.072 69 31 0 182 250 46 114 3.08e-19 86.7
MS.gene017974.t1 AT3G04030 41.379 116 52 2 218 317 1 116 2.91e-18 84.7
MS.gene017974.t1 AT4G04605 45.263 95 48 2 182 276 10 100 5.16e-17 79.3
MS.gene017974.t1 AT2G38300 58.065 62 26 0 182 243 50 111 6.18e-17 81.3
MS.gene017974.t1 AT2G02060 63.793 58 21 0 182 239 25 82 8.17e-17 79.7
MS.gene017974.t1 AT2G02060 63.793 58 21 0 182 239 25 82 8.17e-17 79.7
MS.gene017974.t1 AT4G17695 54.412 68 29 1 188 253 166 233 8.35e-17 80.9
MS.gene017974.t1 AT1G14600 58.065 62 26 0 182 243 19 80 1.55e-16 79.0
MS.gene017974.t1 AT1G32240 54.412 68 29 1 188 253 215 282 1.81e-16 80.1
MS.gene017974.t1 AT1G32240 54.412 68 29 1 188 253 215 282 2.21e-16 80.1
MS.gene017974.t1 AT5G16560 60.000 55 22 0 188 242 221 275 5.75e-16 79.0
MS.gene017974.t1 AT1G79430 38.318 107 60 3 218 319 1 106 1.00e-15 77.0
MS.gene017974.t1 AT5G42630 51.562 64 31 0 188 251 107 170 2.14e-15 75.1
MS.gene017974.t1 AT2G06020 54.386 57 26 0 186 242 86 142 3.09e-15 75.9
MS.gene017974.t1 AT5G42630 51.562 64 31 0 188 251 107 170 3.50e-15 75.5
MS.gene017974.t1 AT2G40260 57.627 59 24 1 185 242 80 138 7.07e-15 75.9
MS.gene017974.t1 AT4G04580 50.820 61 30 0 182 242 10 70 5.04e-14 69.7
MS.gene017974.t1 AT3G04030 37.931 116 50 3 218 317 1 110 1.87e-13 70.9
MS.gene017974.t1 AT4G04555 51.471 68 28 1 180 242 3 70 3.38e-13 65.9
MS.gene017974.t1 AT4G37180 50.000 64 32 0 176 239 200 263 6.57e-13 69.3
MS.gene017974.t1 AT4G37180 50.000 64 32 0 176 239 207 270 7.97e-13 69.3
MS.gene017974.t1 AT5G18240 35.897 117 53 3 218 318 1 111 2.53e-12 67.4
MS.gene017974.t1 AT2G40970 46.053 76 38 2 166 239 83 157 8.78e-12 65.1
MS.gene017974.t1 AT2G03500 49.254 67 31 1 173 239 223 286 2.41e-11 65.1
MS.gene017974.t1 AT5G58080 51.667 60 28 1 180 239 188 246 5.11e-11 64.3

Find 51 sgRNAs with CRISPR-Local

Find 116 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCATCATTGATTGTATT+TGG 0.166617 7.1:-14547919 None:intergenic
AAGGGAAAACAATCATTTAT+TGG 0.215858 7.1:+14545952 MS.gene017974:CDS
TCCCTTTGTGTCAATCTCTA+AGG 0.220544 7.1:+14545933 MS.gene017974:CDS
AATTATGTGTTCGGAGGTTT+TGG 0.254189 7.1:-14548001 None:intergenic
CAGCAAAAGTTGTTCTTTGA+TGG 0.257577 7.1:+14546133 MS.gene017974:CDS
ATCATATAAGGTTAATGATT+TGG 0.267300 7.1:+14545907 MS.gene017974:CDS
ATCATCTTCAATATAGCTTT+TGG 0.290192 7.1:-14546845 None:intergenic
TTTGAGTTCTCAATCATATA+AGG 0.290541 7.1:+14545895 MS.gene017974:CDS
CAAGCTGCACATCATCTATA+TGG 0.344546 7.1:-14547356 None:intergenic
TAATGGAAATCATGACTATA+TGG 0.366206 7.1:+14546192 MS.gene017974:CDS
AAACTTCCTGCTGAAATTGT+TGG 0.389210 7.1:-14546606 None:intergenic
TTGAGTGTGTGAATCGCCTT+GGG 0.396796 7.1:+14546721 MS.gene017974:CDS
GGAAGTTTATCAACTTCTCT+TGG 0.397953 7.1:+14546621 MS.gene017974:CDS
GTGTTAGACAGTTCAAAGTT+GGG 0.442345 7.1:-14545995 None:intergenic
ATAGATGATGTGCAGCTTGT+TGG 0.451741 7.1:+14547360 MS.gene017974:CDS
GTCTAACACTTTGCAAGCAT+TGG 0.457506 7.1:+14546009 MS.gene017974:CDS
GTTGAGTGTGTGAATCGCCT+TGG 0.458117 7.1:+14546720 MS.gene017974:CDS
AGTGTTAGACAGTTCAAAGT+TGG 0.458507 7.1:-14545996 None:intergenic
CAAGTAAAACAAGAATTAGA+TGG 0.463217 7.1:+14546673 MS.gene017974:CDS
TTGCAGTGGCTTTCAAATCA+AGG 0.468098 7.1:+14547533 MS.gene017974:intron
TAACTATCAAATTATGTGTT+CGG 0.471768 7.1:-14548010 None:intergenic
AAAAGCTATATTGAAGATGA+TGG 0.473225 7.1:+14546847 MS.gene017974:CDS
CCCTTAGAGATTGACACAAA+GGG 0.473466 7.1:-14545934 None:intergenic
AAGATTGATTATTATCAACT+TGG 0.481452 7.1:-14545874 None:intergenic
AACAAGAATTAGATGGACTA+AGG 0.489147 7.1:+14546680 MS.gene017974:CDS
TTGGTGAAATCTCAATTGAA+TGG 0.505462 7.1:+14546028 MS.gene017974:CDS
GTTCAAAGTTGGGGTCATGC+TGG 0.509873 7.1:-14545985 None:intergenic
CCCTTTGTGTCAATCTCTAA+GGG 0.510278 7.1:+14545934 MS.gene017974:CDS
CTTGGAAGCACTTCTTCTAG+TGG 0.515481 7.1:+14546639 MS.gene017974:CDS
AAAGATGTTTCTATTTCTGA+AGG 0.518549 7.1:+14547955 MS.gene017974:CDS
TTGTCTCCAACAATTTCAGC+AGG 0.521373 7.1:+14546600 MS.gene017974:CDS
AAATTACCTTGAGCTGACTC+TGG 0.535434 7.1:-14546938 None:intergenic
ATTGGAAAAGGCTTGCTCCC+AGG 0.542042 7.1:-14546106 None:intergenic
CAGAAGCTGGTGACTCGAAA+CGG 0.549320 7.1:-14545805 None:intergenic
AACATTACAATTGCGACTTG+AGG 0.551481 7.1:+14547828 MS.gene017974:CDS
TTGGAAGCACTTCTTCTAGT+GGG 0.558294 7.1:+14546640 MS.gene017974:CDS
TATGCAACAGAAAACTGCAT+GGG 0.559407 7.1:+14545833 MS.gene017974:CDS
ATCCGTTTGCAGGTTGCTCG+TGG 0.560326 7.1:+14546525 MS.gene017974:intron
TCCCTTAGAGATTGACACAA+AGG 0.575040 7.1:-14545935 None:intergenic
CTATCAAATTATGTGTTCGG+AGG 0.576552 7.1:-14548007 None:intergenic
TTATGCAACAGAAAACTGCA+TGG 0.589899 7.1:+14545832 MS.gene017974:CDS
TTGTATTTGGAGTATTGAAG+AGG 0.600647 7.1:-14547906 None:intergenic
TGTTAGACAGTTCAAAGTTG+GGG 0.605516 7.1:-14545994 None:intergenic
GCGTCTTCATGAACAACTAG+AGG 0.618964 7.1:+14547587 MS.gene017974:CDS
TTGAAGATGATGGACTCTGA+AGG 0.627815 7.1:+14546857 MS.gene017974:CDS
TTCATGCCAGAGTCAGCTCA+AGG 0.639842 7.1:+14546932 MS.gene017974:CDS
CAATTGCGACTTGAGGAACA+AGG 0.643962 7.1:+14547835 MS.gene017974:CDS
AACTTCAATTAGATGCACAA+AGG 0.668848 7.1:+14547565 MS.gene017974:CDS
TGATGAGCTGTTGCTAACTG+AGG 0.672832 7.1:-14546161 None:intergenic
GAGTGTGTGAATCGCCTTGG+GGG 0.715113 7.1:+14546723 MS.gene017974:CDS
TGAGTGTGTGAATCGCCTTG+GGG 0.716876 7.1:+14546722 MS.gene017974:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACCTTATTTTTATTTTATAT+GGG + chr7.1:14547745-14547764 MS.gene017974:intron 10.0%
!! AATGATGATACAAAAAATAT+TGG + chr7.1:14547931-14547950 MS.gene017974:CDS 15.0%
!! TCCCATATAAAATAAAAATA+AGG - chr7.1:14547749-14547768 None:intergenic 15.0%
!!! AAAATTTATTCAAGTTTTCA+TGG - chr7.1:14546072-14546091 None:intergenic 15.0%
!!! GACCTTATTTTTATTTTATA+TGG + chr7.1:14547744-14547763 MS.gene017974:intron 15.0%
!! AAGATTGATTATTATCAACT+TGG - chr7.1:14545877-14545896 None:intergenic 20.0%
!! AAGTTCATAAAGAAAATGAA+TGG - chr7.1:14547623-14547642 None:intergenic 20.0%
!! AGTATAAATATGTTATGACA+CGG - chr7.1:14546337-14546356 None:intergenic 20.0%
!! ATCATATAAGGTTAATGATT+TGG + chr7.1:14545907-14545926 MS.gene017974:CDS 20.0%
!! ATCATTAAAATCAGTTTGTA+AGG + chr7.1:14547689-14547708 MS.gene017974:intron 20.0%
!! ATGAAATTAATCAATCAACT+TGG - chr7.1:14547456-14547475 None:intergenic 20.0%
!! TTAGAGTCATATAACATATA+AGG - chr7.1:14547484-14547503 None:intergenic 20.0%
!!! AACTTGAATAAATTTTCATG+TGG + chr7.1:14546076-14546095 MS.gene017974:CDS 20.0%
!!! ACATTAGTTTGTTCAATATT+AGG - chr7.1:14547305-14547324 None:intergenic 20.0%
!!! ACTGATTTTAATGATGATTT+AGG - chr7.1:14547684-14547703 None:intergenic 20.0%
!!! ACTTGAATAAATTTTCATGT+GGG + chr7.1:14546077-14546096 MS.gene017974:CDS 20.0%
!!! TAAGAGTATAAAAGTAGTTT+AGG - chr7.1:14547416-14547435 None:intergenic 20.0%
! AAAAGCTATATTGAAGATGA+TGG + chr7.1:14546847-14546866 MS.gene017974:CDS 25.0%
! AAATTAATCAATCAACTTGG+TGG - chr7.1:14547453-14547472 None:intergenic 25.0%
! AAGGGAAAACAATCATTTAT+TGG + chr7.1:14545952-14545971 MS.gene017974:CDS 25.0%
! ACATTATTCACTATGAACAA+TGG - chr7.1:14547133-14547152 None:intergenic 25.0%
! ATAAATCATCGTATCACATT+AGG + chr7.1:14546469-14546488 MS.gene017974:intron 25.0%
! CAAGTAAAACAAGAATTAGA+TGG + chr7.1:14546673-14546692 MS.gene017974:CDS 25.0%
! CATTATTCACTATGAACAAT+GGG - chr7.1:14547132-14547151 None:intergenic 25.0%
! CTCAACATGAAAAATAAACT+AGG - chr7.1:14547006-14547025 None:intergenic 25.0%
! GAAAGAAAATAAGCACATTT+GGG - chr7.1:14547261-14547280 None:intergenic 25.0%
! TAAAATCAGTTTGTAAGGTA+AGG + chr7.1:14547694-14547713 MS.gene017974:intron 25.0%
! TAATGGAAATCATGACTATA+TGG + chr7.1:14546192-14546211 MS.gene017974:CDS 25.0%
! TGAAAGAAAATAAGCACATT+TGG - chr7.1:14547262-14547281 None:intergenic 25.0%
! TGAATAATGTCTGGTTAAAT+AGG + chr7.1:14547143-14547162 MS.gene017974:intron 25.0%
! TGTATTTATATCCACTAGAA+TGG + chr7.1:14547199-14547218 MS.gene017974:intron 25.0%
!! AGCTCATCATTTTGTAATAA+TGG + chr7.1:14546175-14546194 MS.gene017974:CDS 25.0%
!! ATCATCTTCAATATAGCTTT+TGG - chr7.1:14546848-14546867 None:intergenic 25.0%
!! ATGACTTTTCACATGAAATA+TGG - chr7.1:14546888-14546907 None:intergenic 25.0%
!! GTATCATCATTGATTGTATT+TGG - chr7.1:14547922-14547941 None:intergenic 25.0%
!! TATCTGATAGTCTTTTCTAT+TGG + chr7.1:14546800-14546819 MS.gene017974:intron 25.0%
!! TTTGAGTTCTCAATCATATA+AGG + chr7.1:14545895-14545914 MS.gene017974:CDS 25.0%
!! TTTGTTATAGTGTTTGTCAA+TGG + chr7.1:14547083-14547102 MS.gene017974:intron 25.0%
!!! AAAGATGTTTCTATTTCTGA+AGG + chr7.1:14547955-14547974 MS.gene017974:CDS 25.0%
!!! TAATCATGTTGTTACTTTCT+TGG + chr7.1:14547792-14547811 MS.gene017974:intron 25.0%
AAACTAATGTGTCCAATTTC+AGG + chr7.1:14547315-14547334 MS.gene017974:intron 30.0%
AACTAATGTGTCCAATTTCA+GGG + chr7.1:14547316-14547335 MS.gene017974:intron 30.0%
AACTTCAATTAGATGCACAA+AGG + chr7.1:14547565-14547584 MS.gene017974:CDS 30.0%
CATAAAGAAAATGAATGGCA+AGG - chr7.1:14547618-14547637 None:intergenic 30.0%
TATAGTGTTTGTCAATGGAT+AGG + chr7.1:14547088-14547107 MS.gene017974:intron 30.0%
TGTTCATAGTGAATAATGTC+TGG + chr7.1:14547134-14547153 MS.gene017974:intron 30.0%
TTGGTGAAATCTCAATTGAA+TGG + chr7.1:14546028-14546047 MS.gene017974:CDS 30.0%
TTTGCAAAATGATTCTTCTG+CGG + chr7.1:14546223-14546242 MS.gene017974:intron 30.0%
TTTGTCAATGGATAGGATAT+AGG + chr7.1:14547095-14547114 MS.gene017974:intron 30.0%
! TCAAGTTTTCATGGAATCTA+AGG - chr7.1:14546063-14546082 None:intergenic 30.0%
!! AACAAGAATTAGATGGACTA+AGG + chr7.1:14546680-14546699 MS.gene017974:CDS 30.0%
!! TATTGGTTTCTTGCTTATAG+AGG + chr7.1:14546817-14546836 MS.gene017974:intron 30.0%
!! TTGTATTTGGAGTATTGAAG+AGG - chr7.1:14547909-14547928 None:intergenic 30.0%
!!! AACAACTTTTGCTGTTCAAT+TGG - chr7.1:14546127-14546146 None:intergenic 30.0%
!!! TTTTGCTGTTCAATTGGAAA+AGG - chr7.1:14546121-14546140 None:intergenic 30.0%
AAACTTCCTGCTGAAATTGT+TGG - chr7.1:14546609-14546628 None:intergenic 35.0%
AACATTACAATTGCGACTTG+AGG + chr7.1:14547828-14547847 MS.gene017974:CDS 35.0%
AGTGTTAGACAGTTCAAAGT+TGG - chr7.1:14545999-14546018 None:intergenic 35.0%
CAGCAAAAGTTGTTCTTTGA+TGG + chr7.1:14546133-14546152 MS.gene017974:CDS 35.0%
GGAAGTTTATCAACTTCTCT+TGG + chr7.1:14546621-14546640 MS.gene017974:CDS 35.0%
GTGTTAGACAGTTCAAAGTT+GGG - chr7.1:14545998-14546017 None:intergenic 35.0%
TAATTATAGCATCCGTTTGC+AGG + chr7.1:14546515-14546534 MS.gene017974:intron 35.0%
TATGCAACAGAAAACTGCAT+GGG + chr7.1:14545833-14545852 MS.gene017974:CDS 35.0%
TCAAACTTCTACCATTCTAG+TGG - chr7.1:14547213-14547232 None:intergenic 35.0%
TGTTAGACAGTTCAAAGTTG+GGG - chr7.1:14545997-14546016 None:intergenic 35.0%
TTATGCAACAGAAAACTGCA+TGG + chr7.1:14545832-14545851 MS.gene017974:CDS 35.0%
TTGTCAGATTTCCCTGAAAT+TGG - chr7.1:14547330-14547349 None:intergenic 35.0%
! AATTATGTGTTCGGAGGTTT+TGG - chr7.1:14548004-14548023 None:intergenic 35.0%
! TTTCATGTGGGAATTTTCCT+GGG + chr7.1:14546089-14546108 MS.gene017974:CDS 35.0%
! TTTTCATGTGGGAATTTTCC+TGG + chr7.1:14546088-14546107 MS.gene017974:CDS 35.0%
AACGATCAAGATACTACTGG+AGG + chr7.1:14546289-14546308 MS.gene017974:intron 40.0%
ATAGATGATGTGCAGCTTGT+TGG + chr7.1:14547360-14547379 MS.gene017974:CDS 40.0%
CAAGCTGCACATCATCTATA+TGG - chr7.1:14547359-14547378 None:intergenic 40.0%
CCCTTAGAGATTGACACAAA+GGG - chr7.1:14545937-14545956 None:intergenic 40.0%
CCCTTTGTGTCAATCTCTAA+GGG + chr7.1:14545934-14545953 MS.gene017974:CDS 40.0%
GTCTAACACTTTGCAAGCAT+TGG + chr7.1:14546009-14546028 MS.gene017974:CDS 40.0%
GTTGCATAAAAAGCAGAAGC+TGG - chr7.1:14545821-14545840 None:intergenic 40.0%
TCCAACGATCAAGATACTAC+TGG + chr7.1:14546286-14546305 MS.gene017974:intron 40.0%
TCCAGTAGTATCTTGATCGT+TGG - chr7.1:14546290-14546309 None:intergenic 40.0%
TCCCTTAGAGATTGACACAA+AGG - chr7.1:14545938-14545957 None:intergenic 40.0%
TCCCTTTGTGTCAATCTCTA+AGG + chr7.1:14545933-14545952 MS.gene017974:CDS 40.0%
TTGAGAATCCTTGCATCTCA+CGG + chr7.1:14546360-14546379 MS.gene017974:intron 40.0%
TTGCAGTGGCTTTCAAATCA+AGG + chr7.1:14547533-14547552 MS.gene017974:intron 40.0%
TTGTCTCCAACAATTTCAGC+AGG + chr7.1:14546600-14546619 MS.gene017974:CDS 40.0%
! AAATTACCTTGAGCTGACTC+TGG - chr7.1:14546941-14546960 None:intergenic 40.0%
!! TTGAAGATGATGGACTCTGA+AGG + chr7.1:14546857-14546876 MS.gene017974:CDS 40.0%
!! TTGGAAGCACTTCTTCTAGT+GGG + chr7.1:14546640-14546659 MS.gene017974:CDS 40.0%
AAGCCACTGCAAATCAAGAG+TGG - chr7.1:14547525-14547544 None:intergenic 45.0%
CAATTGCGACTTGAGGAACA+AGG + chr7.1:14547835-14547854 MS.gene017974:CDS 45.0%
GATCAAGATACTACTGGAGG+TGG + chr7.1:14546292-14546311 MS.gene017974:intron 45.0%
GCGTCTTCATGAACAACTAG+AGG + chr7.1:14547587-14547606 MS.gene017974:CDS 45.0%
GTTATGACACGGATCCTCTT+CGG - chr7.1:14546326-14546345 None:intergenic 45.0%
TGAGAATCCTTGCATCTCAC+GGG + chr7.1:14546361-14546380 MS.gene017974:intron 45.0%
TGATGAGCTGTTGCTAACTG+AGG - chr7.1:14546164-14546183 None:intergenic 45.0%
TTGAGTGTGTGAATCGCCTT+GGG + chr7.1:14546721-14546740 MS.gene017974:CDS 45.0%
!! ACACCACTCTTGATTTGCAG+TGG + chr7.1:14547519-14547538 MS.gene017974:intron 45.0%
!! CTTGGAAGCACTTCTTCTAG+TGG + chr7.1:14546639-14546658 MS.gene017974:CDS 45.0%
!!! TGTGTTCGGAGGTTTTGGAT+TGG - chr7.1:14547999-14548018 None:intergenic 45.0%
ATCCACGAGCAACCTGCAAA+CGG - chr7.1:14546530-14546549 None:intergenic 50.0%
ATTGAACGACCAAGCTCTGC+TGG - chr7.1:14546263-14546282 None:intergenic 50.0%
CAAGATACTACTGGAGGTGG+TGG + chr7.1:14546295-14546314 MS.gene017974:intron 50.0%
CAGAAGCTGGTGACTCGAAA+CGG - chr7.1:14545808-14545827 None:intergenic 50.0%
GAGAATCCTTGCATCTCACG+GGG + chr7.1:14546362-14546381 MS.gene017974:intron 50.0%
GTTCAAAGTTGGGGTCATGC+TGG - chr7.1:14545988-14546007 None:intergenic 50.0%
GTTGAGTGTGTGAATCGCCT+TGG + chr7.1:14546720-14546739 MS.gene017974:CDS 50.0%
TGAGTGTGTGAATCGCCTTG+GGG + chr7.1:14546722-14546741 MS.gene017974:CDS 50.0%
TTCATGCCAGAGTCAGCTCA+AGG + chr7.1:14546932-14546951 MS.gene017974:CDS 50.0%
TTTCTTCCCCGTGAGATGCA+AGG - chr7.1:14546371-14546390 None:intergenic 50.0%
! ATTGGAAAAGGCTTGCTCCC+AGG - chr7.1:14546109-14546128 None:intergenic 50.0%
ACTTACTCTCAGCACCCCCA+AGG - chr7.1:14546740-14546759 None:intergenic 55.0%
ATCCGTTTGCAGGTTGCTCG+TGG + chr7.1:14546525-14546544 MS.gene017974:intron 55.0%
CTCTGCTGGAGAGGTAGAAG+AGG - chr7.1:14546249-14546268 None:intergenic 55.0%
GAGTGTGTGAATCGCCTTGG+GGG + chr7.1:14546723-14546742 MS.gene017974:CDS 55.0%
TCTACCTCTCCAGCAGAGCT+TGG + chr7.1:14546251-14546270 MS.gene017974:intron 55.0%
ACGACCAAGCTCTGCTGGAG+AGG - chr7.1:14546258-14546277 None:intergenic 60.0%
!! AGGTGGTGGAGAGACCGAAG+AGG + chr7.1:14546309-14546328 MS.gene017974:intron 60.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 14545800 14548026 14545800 ID=MS.gene017974
chr7.1 mRNA 14545800 14548026 14545800 ID=MS.gene017974.t1;Parent=MS.gene017974
chr7.1 exon 14545800 14546213 14545800 ID=MS.gene017974.t1.exon1;Parent=MS.gene017974.t1
chr7.1 CDS 14545800 14546213 14545800 ID=cds.MS.gene017974.t1;Parent=MS.gene017974.t1
chr7.1 exon 14546537 14546753 14546537 ID=MS.gene017974.t1.exon2;Parent=MS.gene017974.t1
chr7.1 CDS 14546537 14546753 14546537 ID=cds.MS.gene017974.t1;Parent=MS.gene017974.t1
chr7.1 exon 14546837 14546953 14546837 ID=MS.gene017974.t1.exon3;Parent=MS.gene017974.t1
chr7.1 CDS 14546837 14546953 14546837 ID=cds.MS.gene017974.t1;Parent=MS.gene017974.t1
chr7.1 exon 14547337 14547391 14547337 ID=MS.gene017974.t1.exon4;Parent=MS.gene017974.t1
chr7.1 CDS 14547337 14547391 14547337 ID=cds.MS.gene017974.t1;Parent=MS.gene017974.t1
chr7.1 exon 14547539 14547608 14547539 ID=MS.gene017974.t1.exon5;Parent=MS.gene017974.t1
chr7.1 CDS 14547539 14547608 14547539 ID=cds.MS.gene017974.t1;Parent=MS.gene017974.t1
chr7.1 exon 14547820 14548026 14547820 ID=MS.gene017974.t1.exon6;Parent=MS.gene017974.t1
chr7.1 CDS 14547820 14548026 14547820 ID=cds.MS.gene017974.t1;Parent=MS.gene017974.t1
Gene Sequence

>MS.gene017974

ATGAGCCGTTTCGAGTCACCAGCTTCTGCTTTTTATGCAACAGAAAACTGCATGGGATTTGCAGAATTTGATCACCAAGTTGATAATAATCAATCTTTGAGTTCTCAATCATATAAGGTTAATGATTTGGAATTCCCTTTGTGTCAATCTCTAAGGGAAAACAATCATTTATTGGATGCATCAAACCAGCATGACCCCAACTTTGAACTGTCTAACACTTTGCAAGCATTGGTGAAATCTCAATTGAATGGTAATCAACACCTTAGATTCCATGAAAACTTGAATAAATTTTCATGTGGGAATTTTCCTGGGAGCAAGCCTTTTCCAATTGAACAGCAAAAGTTGTTCTTTGATGGTCTTGCCTCAGTTAGCAACAGCTCATCATTTTGTAATAATGGAAATCATGACTATATGGTAAGTGATTTTGCAAAATGATTCTTCTGCGGCCTCTTCTACCTCTCCAGCAGAGCTTGGTCGTTCAATTAATCCAACGATCAAGATACTACTGGAGGTGGTGGAGAGACCGAAGAGGATCCGTGTCATAACATATTTATACTATATTGAGAATCCTTGCATCTCACGGGGAAGAAATCGTCGAAAGAATATATATATATTAGTTACGCTTTATACTTTTTTGTGTTCAATTATTGAGCATCAATAACATCTAACATAAATCATCGTATCACATTAGGCATACATCAATGAATCACATGTCTAATTATAGCATCCGTTTGCAGGTTGCTCGTGGATCTTATCATTTATCTGTTGAGCAACTGAACTTCTCTTCTCAGCATGAGAAGTTGTCTCCAACAATTTCAGCAGGAAGTTTATCAACTTCTCTTGGAAGCACTTCTTCTAGTGGGAATGTAGTCTCAAGTAAAACAAGAATTAGATGGACTAAGGATCTTCATGAGAAGTTTGTTGAGTGTGTGAATCGCCTTGGGGGTGCTGAGAGTAAGTATTGCTTTCAAAATTTGTCAATTCAAGAAACAAATGTTAATATCTGATAGTCTTTTCTATTGGTTTCTTGCTTATAGAGGCAACACCAAAAGCTATATTGAAGATGATGGACTCTGAAGGCTTGACCATATTTCATGTGAAAAGTCATTTGCAGAAATATAGAACTGCAAAATTCATGCCAGAGTCAGCTCAAGGTAATTTCTCTTTTTCTTTAGCATCATTGTAGACTTCTAGTGTAGTTTGCCTAGTTTATTTTTCATGTTGAGTATATTTTTTGTATTTTTGAATTTACTAGCATTCATGTCATAAGTGATAAGCAAATGTTTGTTATAGTGTTTGTCAATGGATAGGATATAGGTTTATCTTTTTCCCATTGTTCATAGTGAATAATGTCTGGTTAAATAGGCTTCAAATATAAGAAGTTGCAATGCAGAATAAATGTATTTATATCCACTAGAATGGTAGAAGTTTGAACTTCAATTATTCATAAACTATTGTCCCAAATGTGCTTATTTTCTTTCATATTATCTATCATATTGACTCCTAATATTGAACAAACTAATGTGTCCAATTTCAGGGAAATCTGACAAAAGAATCCATATAGATGATGTGCAGCTTGTTGGTGTTAAAACGTAATGCTATTTTTTCTTTTACCTAAACTACTTTTATACTCTTATGATTTTAGTATTTCCACCAAGTTGATTGATTAATTTCATATGAACCTTATATGTTATATGACTCTAAAAATCTACTAAATTTACACCACTCTTGATTTGCAGTGGCTTTCAAATCAAGGAAGCACTTCAACTTCAATTAGATGCACAAAGGCGTCTTCATGAACAACTAGAGGTATATCCTTGCCATTCATTTTCTTTATGAACTTTGTTGAACTCTCATACTATATTGAATTTTTATATTGAACCTAAATCATCATTAAAATCAGTTTGTAAGGTAAGGATCGTCACTCGTTATAAACTCATTTCAGACCTTATTTTTATTTTATATGGGACTTACTTTTATCTAATACTTTTCTAATCATGTTGTTACTTTCTTGGTCTAGATTCAAAGAACATTACAATTGCGACTTGAGGAACAAGGCAGACAGCTGAAAAAAATGTTTGATCAGCAGCAGAAGACATGTAGTAACCTCTTCAATACTCCAAATACAATCAATGATGATACAAAAAATATTGGCAAAGATGTTTCTATTTCTGAAGGAGCTGAAAACTCTTTATTCCAATCCAAAACCTCCGAACACATAATTTGA
Protein sequence

>MS.gene017974.t1

MSRFESPASAFYATENCMGFAEFDHQVDNNQSLSSQSYKVNDLEFPLCQSLRENNHLLDASNQHDPNFELSNTLQALVKSQLNGNQHLRFHENLNKFSCGNFPGSKPFPIEQQKLFFDGLASVSNSSSFCNNGNHDYMVARGSYHLSVEQLNFSSQHEKLSPTISAGSLSTSLGSTSSSGNVVSSKTRIRWTKDLHEKFVECVNRLGGAEKATPKAILKMMDSEGLTIFHVKSHLQKYRTAKFMPESAQGKSDKRIHIDDVQLVGVKTGFQIKEALQLQLDAQRRLHEQLEIQRTLQLRLEEQGRQLKKMFDQQQKTCSNLFNTPNTINDDTKNIGKDVSISEGAENSLFQSKTSEHII