Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017984.t1 | XP_013466755.2 | 57 | 165 | 39 | 2 | 34 | 166 | 645 | 809 | 6.90E-43 | 183.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017984.t1 | A0A396JLZ7 | 66.7 | 105 | 35 | 0 | 40 | 144 | 4 | 108 | 1.5e-34 | 155.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene017984.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene017984.t1 | MTR_1g035640 | 51.020 | 98 | 48 | 0 | 40 | 137 | 165 | 262 | 4.19e-26 | 103 |
| MS.gene017984.t1 | MTR_1g050845 | 52.174 | 92 | 40 | 3 | 50 | 137 | 158 | 249 | 2.20e-19 | 84.7 |
| MS.gene017984.t1 | MTR_1g035570 | 43.333 | 90 | 51 | 0 | 48 | 137 | 180 | 269 | 4.07e-19 | 83.6 |
| MS.gene017984.t1 | MTR_1g035620 | 49.020 | 102 | 45 | 4 | 40 | 137 | 163 | 261 | 7.71e-19 | 82.8 |
| MS.gene017984.t1 | MTR_1g035490 | 46.341 | 82 | 44 | 0 | 56 | 137 | 97 | 178 | 2.36e-17 | 77.8 |
| MS.gene017984.t1 | MTR_6g061070 | 43.750 | 112 | 54 | 4 | 35 | 137 | 193 | 304 | 1.23e-16 | 76.6 |
| MS.gene017984.t1 | MTR_5g044670 | 36.957 | 92 | 55 | 2 | 56 | 146 | 205 | 294 | 8.39e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 107 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCAATTATTGAACATCTCTT+AGG | 0.278157 | 7.1:+14419174 | None:intergenic |
| GGGACCCCTTCTACAAATTC+AGG | 0.347036 | 7.1:-14418579 | MS.gene017984:CDS |
| GAACCTCCTGAATTTGTAGA+AGG | 0.363430 | 7.1:+14418573 | None:intergenic |
| TTGACGGGATCGCAAGGATA+AGG | 0.384450 | 7.1:-14418955 | MS.gene017984:CDS |
| ACTCACGAAGTTGAATAGTT+TGG | 0.408216 | 7.1:+14420358 | None:intergenic |
| TTCCCTTAGCATCACTGCCT+TGG | 0.419148 | 7.1:+14420391 | None:intergenic |
| TGACGGGATCGCAAGGATAA+GGG | 0.470585 | 7.1:-14418954 | MS.gene017984:CDS |
| ATCACTGCCTTGGTGAATGC+AGG | 0.481007 | 7.1:+14420401 | None:intergenic |
| AATGTCAGTGTCTAGTTGAA+TGG | 0.484492 | 7.1:+14420434 | None:intergenic |
| TCACCAAGGCAGTGATGCTA+AGG | 0.490808 | 7.1:-14420394 | MS.gene017984:CDS |
| AATACTTGAACGAGTTTGAC+GGG | 0.492831 | 7.1:-14418970 | MS.gene017984:CDS |
| AAGGAAAATAACTTGCAAGT+TGG | 0.532958 | 7.1:-14418842 | MS.gene017984:CDS |
| GGGTTGATGCAGGTAGAACT+TGG | 0.542033 | 7.1:-14418934 | MS.gene017984:CDS |
| GAATACTTGAACGAGTTTGA+CGG | 0.544212 | 7.1:-14418971 | MS.gene017984:CDS |
| AACCTCCTGAATTTGTAGAA+GGG | 0.561604 | 7.1:+14418574 | None:intergenic |
| ACCTCCTGAATTTGTAGAAG+GGG | 0.563047 | 7.1:+14418575 | None:intergenic |
| ACGAGTTTGACGGGATCGCA+AGG | 0.567127 | 7.1:-14418961 | MS.gene017984:CDS |
| GCAAGGATAAGGGTTGATGC+AGG | 0.581356 | 7.1:-14418944 | MS.gene017984:CDS |
| AACTTTGCAGGTACTAACAA+AGG | 0.591292 | 7.1:-14419207 | MS.gene017984:intron |
| GAACTTGGAAAGTGAATGTG+AGG | 0.592866 | 7.1:-14418919 | MS.gene017984:CDS |
| AATTAATCATACCGCGTACG+AGG | 0.595783 | 7.1:+14418509 | None:intergenic |
| GCAGGTACTAACAAAGGAGG+AGG | 0.600379 | 7.1:-14419201 | MS.gene017984:intron |
| TGGTTGGTCTTTATTCAACA+AGG | 0.607895 | 7.1:-14418861 | MS.gene017984:CDS |
| ATGACACAAACTTATGCCCA+TGG | 0.615053 | 7.1:-14419102 | MS.gene017984:CDS |
| CACCAAGGCAGTGATGCTAA+GGG | 0.633760 | 7.1:-14420393 | MS.gene017984:CDS |
| ACCCCTTCTACAAATTCAGG+AGG | 0.639356 | 7.1:-14418576 | MS.gene017984:CDS |
| TTTGCAGGTACTAACAAAGG+AGG | 0.671084 | 7.1:-14419204 | MS.gene017984:intron |
| CAACATACCTGCATTCACCA+AGG | 0.681970 | 7.1:-14420408 | MS.gene017984:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATAATCAATAGTAAT+TGG | + | chr7.1:14419605-14419624 | None:intergenic | 10.0% |
| !!! | ATTGTTTTTATAATAATGAT+CGG | - | chr7.1:14418877-14418896 | MS.gene017984:CDS | 10.0% |
| !! | ACAAATCAAAAATATACAAA+TGG | + | chr7.1:14419063-14419082 | None:intergenic | 15.0% |
| !!! | ATTTGTATATTTTTGATTTG+TGG | - | chr7.1:14419062-14419081 | MS.gene017984:intron | 15.0% |
| !!! | TATTCTTTTGAAAACTTATT+TGG | + | chr7.1:14419967-14419986 | None:intergenic | 15.0% |
| !!! | TTGAAAATGTTTACTAATTT+TGG | - | chr7.1:14419896-14419915 | MS.gene017984:intron | 15.0% |
| !! | AAACAATAAAATGTTCATTC+AGG | + | chr7.1:14419022-14419041 | None:intergenic | 20.0% |
| !! | AATCAAAAATATACAAATGG+AGG | + | chr7.1:14419060-14419079 | None:intergenic | 20.0% |
| !! | AGTGATAAAAGGAATTAAAA+AGG | + | chr7.1:14420337-14420356 | None:intergenic | 20.0% |
| !! | ATAGAAATATTGAATGAAAG+CGG | + | chr7.1:14420171-14420190 | None:intergenic | 20.0% |
| !!! | AGTCTAAGATGATTTAAAAA+TGG | + | chr7.1:14419264-14419283 | None:intergenic | 20.0% |
| !!! | ATAATGAGTCTTGTTTATTA+AGG | + | chr7.1:14418728-14418747 | None:intergenic | 20.0% |
| !!! | CTGTATATTGATTTTGTATT+TGG | - | chr7.1:14419337-14419356 | MS.gene017984:intron | 20.0% |
| ! | AAAACCAATGAGTTTAAACT+TGG | + | chr7.1:14419407-14419426 | None:intergenic | 25.0% |
| ! | AAAGCAAACTTACACAAAAA+TGG | + | chr7.1:14419877-14419896 | None:intergenic | 25.0% |
| ! | AACTTTCACAATTGTAAGTT+TGG | - | chr7.1:14419197-14419216 | MS.gene017984:CDS | 25.0% |
| ! | ACTGACTAGGTTATAAAAAA+GGG | - | chr7.1:14420354-14420373 | MS.gene017984:intron | 25.0% |
| ! | AGAATATACATTAACTTTGC+AGG | - | chr7.1:14419734-14419753 | MS.gene017984:intron | 25.0% |
| ! | ATTGAACAAGTAATGAACTT+TGG | + | chr7.1:14419384-14419403 | None:intergenic | 25.0% |
| ! | GACTTATCATGTTAGTAATT+TGG | - | chr7.1:14419280-14419299 | MS.gene017984:intron | 25.0% |
| ! | GATGATTTAAAAATGGACAA+TGG | + | chr7.1:14419257-14419276 | None:intergenic | 25.0% |
| ! | GTATATTCTAATCATTTCCA+AGG | + | chr7.1:14419723-14419742 | None:intergenic | 25.0% |
| ! | TATCTCTCTACAAAATTCTT+TGG | + | chr7.1:14419453-14419472 | None:intergenic | 25.0% |
| ! | TCAATTATTGAACATCTCTT+AGG | + | chr7.1:14419782-14419801 | None:intergenic | 25.0% |
| ! | TTAAAAATGGACAATGGTTT+AGG | + | chr7.1:14419251-14419270 | None:intergenic | 25.0% |
| !! | AAAACTTGGATTCTCATTTT+TGG | + | chr7.1:14419822-14419841 | None:intergenic | 25.0% |
| !! | AATGGTTTAGGTTACAAAAA+TGG | + | chr7.1:14419239-14419258 | None:intergenic | 25.0% |
| !! | AGAATTTTGTAGAGAGATAT+TGG | - | chr7.1:14419454-14419473 | MS.gene017984:intron | 25.0% |
| !!! | GTCATTTTAAGTTCAAAACT+TGG | + | chr7.1:14419836-14419855 | None:intergenic | 25.0% |
| !!! | TTTTGTATTTGGTTTGAGTT+TGG | - | chr7.1:14419348-14419367 | MS.gene017984:intron | 25.0% |
| AAGAGAAACTAATGAAGTAG+AGG | + | chr7.1:14419581-14419600 | None:intergenic | 30.0% | |
| AATATTAAGTAGTCGCATAG+TGG | - | chr7.1:14418904-14418923 | MS.gene017984:CDS | 30.0% | |
| ATAACCTAGTCAGTGATAAA+AGG | + | chr7.1:14420348-14420367 | None:intergenic | 30.0% | |
| ATGATATATCATAGCTTGAG+TGG | + | chr7.1:14418809-14418828 | None:intergenic | 30.0% | |
| CAATCCAAGTTTAAACTCAT+TGG | - | chr7.1:14419400-14419419 | MS.gene017984:intron | 30.0% | |
| TATACCATGAATTGAAACAG+AGG | - | chr7.1:14419938-14419957 | MS.gene017984:intron | 30.0% | |
| TGATATATCATAGCTTGAGT+GGG | + | chr7.1:14418808-14418827 | None:intergenic | 30.0% | |
| ! | AACATCTCTAGTCGAATTTT+AGG | - | chr7.1:14418955-14418974 | MS.gene017984:CDS | 30.0% |
| ! | AAGGAAAATAACTTGCAAGT+TGG | - | chr7.1:14420111-14420130 | MS.gene017984:intron | 30.0% |
| ! | ACATCTCTAGTCGAATTTTA+GGG | - | chr7.1:14418956-14418975 | MS.gene017984:CDS | 30.0% |
| ! | CACTGACTAGGTTATAAAAA+AGG | - | chr7.1:14420353-14420372 | MS.gene017984:intron | 30.0% |
| !! | AAAAACCTTGCAACTTGTTA+GGG | + | chr7.1:14420218-14420237 | None:intergenic | 30.0% |
| !! | AATTCCTTTTATCACTGACT+AGG | - | chr7.1:14420341-14420360 | MS.gene017984:intron | 30.0% |
| !! | GTAATTTGGTTAGCTCTTTT+CGG | - | chr7.1:14419294-14419313 | MS.gene017984:intron | 30.0% |
| !!! | ACTTGCCTAAGACTTTTTTA+TGG | + | chr7.1:14419557-14419576 | None:intergenic | 30.0% |
| !!! | AGATGTTCTTTTTTGCAAGT+TGG | - | chr7.1:14420072-14420091 | MS.gene017984:intron | 30.0% |
| !!! | CTTGCCTAAGACTTTTTTAT+GGG | + | chr7.1:14419556-14419575 | None:intergenic | 30.0% |
| AACCTCCTGAATTTGTAGAA+GGG | + | chr7.1:14420382-14420401 | None:intergenic | 35.0% | |
| AACTTACACAAAAATGGCCA+TGG | + | chr7.1:14419871-14419890 | None:intergenic | 35.0% | |
| AACTTTGCAGGTACTAACAA+AGG | - | chr7.1:14419746-14419765 | MS.gene017984:intron | 35.0% | |
| AATACTTGAACGAGTTTGAC+GGG | - | chr7.1:14419983-14420002 | MS.gene017984:intron | 35.0% | |
| AATGTCAGTGTCTAGTTGAA+TGG | + | chr7.1:14418522-14418541 | None:intergenic | 35.0% | |
| ACTCACGAAGTTGAATAGTT+TGG | + | chr7.1:14418598-14418617 | None:intergenic | 35.0% | |
| ACTTACACAAAAATGGCCAT+GGG | + | chr7.1:14419870-14419889 | None:intergenic | 35.0% | |
| AGTTGTCAGGGTTGAAATAA+TGG | + | chr7.1:14418783-14418802 | None:intergenic | 35.0% | |
| ATAGTGTTCTGGTGTGATAA+AGG | + | chr7.1:14419121-14419140 | None:intergenic | 35.0% | |
| GAATACTTGAACGAGTTTGA+CGG | - | chr7.1:14419982-14420001 | MS.gene017984:intron | 35.0% | |
| GCAACCCATAAAAAAGTCTT+AGG | - | chr7.1:14419549-14419568 | MS.gene017984:intron | 35.0% | |
| GTATCCTCTGTTTCAATTCA+TGG | + | chr7.1:14419945-14419964 | None:intergenic | 35.0% | |
| GTGAACATGTAATAGTGTTC+TGG | + | chr7.1:14419132-14419151 | None:intergenic | 35.0% | |
| GTTGTCAGGGTTGAAATAAT+GGG | + | chr7.1:14418782-14418801 | None:intergenic | 35.0% | |
| TGTGAAAGTTTGTAAAAGCG+TGG | + | chr7.1:14419187-14419206 | None:intergenic | 35.0% | |
| ! | CATCTCTAGTCGAATTTTAG+GGG | - | chr7.1:14418957-14418976 | MS.gene017984:CDS | 35.0% |
| ! | GAAAAACCTTGCAACTTGTT+AGG | + | chr7.1:14420219-14420238 | None:intergenic | 35.0% |
| ! | GTGAAAGTTTGTAAAAGCGT+GGG | + | chr7.1:14419186-14419205 | None:intergenic | 35.0% |
| !! | TGGTTGGTCTTTATTCAACA+AGG | - | chr7.1:14420092-14420111 | MS.gene017984:intron | 35.0% |
| !!! | GTTCTTTTTTGCAAGTTGGT+TGG | - | chr7.1:14420076-14420095 | MS.gene017984:intron | 35.0% |
| ACCTCCTGAATTTGTAGAAG+GGG | + | chr7.1:14420381-14420400 | None:intergenic | 40.0% | |
| ATGACACAAACTTATGCCCA+TGG | - | chr7.1:14419851-14419870 | MS.gene017984:intron | 40.0% | |
| CAATATCCCTTAGACCTCAA+GGG | - | chr7.1:14419493-14419512 | MS.gene017984:intron | 40.0% | |
| GAACCTCCTGAATTTGTAGA+AGG | + | chr7.1:14420383-14420402 | None:intergenic | 40.0% | |
| GAACTTGGAAAGTGAATGTG+AGG | - | chr7.1:14420034-14420053 | MS.gene017984:intron | 40.0% | |
| TCAATATACAGATCCCTTGC+CGG | + | chr7.1:14419328-14419347 | None:intergenic | 40.0% | |
| TTGTCAATGTTTGTCCCTTG+AGG | + | chr7.1:14419510-14419529 | None:intergenic | 40.0% | |
| TTTGCAGGTACTAACAAAGG+AGG | - | chr7.1:14419749-14419768 | MS.gene017984:intron | 40.0% | |
| ! | TTTTGTGAATTCCTCGTACG+CGG | - | chr7.1:14420433-14420452 | MS.gene017984:CDS | 40.0% |
| ACCCCTTCTACAAATTCAGG+AGG | - | chr7.1:14420377-14420396 | MS.gene017984:CDS | 45.0% | |
| CAACATACCTGCATTCACCA+AGG | - | chr7.1:14418545-14418564 | MS.gene017984:CDS | 45.0% | |
| CCAATATCCCTTAGACCTCA+AGG | - | chr7.1:14419492-14419511 | MS.gene017984:intron | 45.0% | |
| CCTTGAGGTCTAAGGGATAT+TGG | + | chr7.1:14419495-14419514 | None:intergenic | 45.0% | |
| GTTTGTCCCTTGAGGTCTAA+GGG | + | chr7.1:14419502-14419521 | None:intergenic | 45.0% | |
| TGTTTGTCCCTTGAGGTCTA+AGG | + | chr7.1:14419503-14419522 | None:intergenic | 45.0% | |
| TTCTATTACCCGAGCTAGTG+AGG | - | chr7.1:14420185-14420204 | MS.gene017984:intron | 45.0% | |
| ! | GTTTGTAAAAGCGTGGGAGT+CGG | + | chr7.1:14419180-14419199 | None:intergenic | 45.0% |
| ATCACTGCCTTGGTGAATGC+AGG | + | chr7.1:14418555-14418574 | None:intergenic | 50.0% | |
| CACCAAGGCAGTGATGCTAA+GGG | - | chr7.1:14418560-14418579 | MS.gene017984:CDS | 50.0% | |
| CTAAAGCTAACTGTGCGCCT+TGG | - | chr7.1:14419703-14419722 | MS.gene017984:intron | 50.0% | |
| GCAGGTACTAACAAAGGAGG+AGG | - | chr7.1:14419752-14419771 | MS.gene017984:intron | 50.0% | |
| GGGACCCCTTCTACAAATTC+AGG | - | chr7.1:14420374-14420393 | MS.gene017984:CDS | 50.0% | |
| TCACCAAGGCAGTGATGCTA+AGG | - | chr7.1:14418559-14418578 | MS.gene017984:CDS | 50.0% | |
| TGACGGGATCGCAAGGATAA+GGG | - | chr7.1:14419999-14420018 | MS.gene017984:intron | 50.0% | |
| TTCCCTTAGCATCACTGCCT+TGG | + | chr7.1:14418565-14418584 | None:intergenic | 50.0% | |
| TTGACGGGATCGCAAGGATA+AGG | - | chr7.1:14419998-14420017 | MS.gene017984:intron | 50.0% | |
| ! | TGAGCGTGCTTACTAGCTCT+TGG | + | chr7.1:14418628-14418647 | None:intergenic | 50.0% |
| !! | CCTTGCAACTTGTTAGGGCT+CGG | + | chr7.1:14420213-14420232 | None:intergenic | 50.0% |
| !! | CTTGAGTGGGCTAGTTGTCA+GGG | + | chr7.1:14418795-14418814 | None:intergenic | 50.0% |
| !! | GCAAGGATAAGGGTTGATGC+AGG | - | chr7.1:14420009-14420028 | MS.gene017984:intron | 50.0% |
| !! | GGGTTGATGCAGGTAGAACT+TGG | - | chr7.1:14420019-14420038 | MS.gene017984:intron | 50.0% |
| ACGAGTTTGACGGGATCGCA+AGG | - | chr7.1:14419992-14420011 | MS.gene017984:intron | 55.0% | |
| ATACAGATCCCTTGCCGGTG+AGG | + | chr7.1:14419323-14419342 | None:intergenic | 55.0% | |
| CCGAGCCCTAACAAGTTGCA+AGG | - | chr7.1:14420210-14420229 | MS.gene017984:intron | 55.0% | |
| !! | GCTTGAGTGGGCTAGTTGTC+AGG | + | chr7.1:14418796-14418815 | None:intergenic | 55.0% |
| GCTCGGTTCCTCACTAGCTC+GGG | + | chr7.1:14420196-14420215 | None:intergenic | 60.0% | |
| ! | GCTCTTTTCGGCGTCCTCAC+CGG | - | chr7.1:14419306-14419325 | MS.gene017984:intron | 60.0% |
| ! | GGCTCGGTTCCTCACTAGCT+CGG | + | chr7.1:14420197-14420216 | None:intergenic | 60.0% |
| TTCGGCGTCCTCACCGGCAA+GGG | - | chr7.1:14419312-14419331 | MS.gene017984:intron | 65.0% | |
| TTTCGGCGTCCTCACCGGCA+AGG | - | chr7.1:14419311-14419330 | MS.gene017984:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 14418515 | 14420460 | 14418515 | ID=MS.gene017984 |
| chr7.1 | mRNA | 14418515 | 14420460 | 14418515 | ID=MS.gene017984.t1;Parent=MS.gene017984 |
| chr7.1 | exon | 14420364 | 14420460 | 14420364 | ID=MS.gene017984.t1.exon1;Parent=MS.gene017984.t1 |
| chr7.1 | CDS | 14420364 | 14420460 | 14420364 | ID=cds.MS.gene017984.t1;Parent=MS.gene017984.t1 |
| chr7.1 | exon | 14419092 | 14419219 | 14419092 | ID=MS.gene017984.t1.exon2;Parent=MS.gene017984.t1 |
| chr7.1 | CDS | 14419092 | 14419219 | 14419092 | ID=cds.MS.gene017984.t1;Parent=MS.gene017984.t1 |
| chr7.1 | exon | 14418834 | 14419017 | 14418834 | ID=MS.gene017984.t1.exon3;Parent=MS.gene017984.t1 |
| chr7.1 | CDS | 14418834 | 14419017 | 14418834 | ID=cds.MS.gene017984.t1;Parent=MS.gene017984.t1 |
| chr7.1 | exon | 14418515 | 14418612 | 14418515 | ID=MS.gene017984.t1.exon4;Parent=MS.gene017984.t1 |
| chr7.1 | CDS | 14418515 | 14418612 | 14418515 | ID=cds.MS.gene017984.t1;Parent=MS.gene017984.t1 |
| Gene Sequence |
| Protein sequence |
