Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018017.t1 | AES81523.1 | 70.4 | 179 | 24 | 3 | 9 | 186 | 11 | 161 | 1.50E-59 | 239.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018017.t1 | G7KWB3 | 70.4 | 179 | 24 | 3 | 9 | 186 | 11 | 161 | 1.1e-59 | 239.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018017.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018017.t1 | MTR_7g093700 | 68.254 | 189 | 29 | 4 | 1 | 186 | 1 | 161 | 1.76e-71 | 213 |
| MS.gene018017.t1 | MTR_7g093670 | 91.463 | 82 | 6 | 1 | 106 | 186 | 1 | 82 | 4.88e-47 | 149 |
| MS.gene018017.t1 | MTR_7g093690 | 56.061 | 132 | 41 | 5 | 70 | 186 | 7 | 136 | 2.90e-31 | 110 |
| MS.gene018017.t1 | MTR_3g011210 | 40.769 | 130 | 49 | 4 | 71 | 175 | 7 | 133 | 1.12e-17 | 75.5 |
| MS.gene018017.t1 | MTR_7g093660 | 54.545 | 77 | 21 | 2 | 123 | 186 | 1 | 76 | 1.01e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATAATATTGATGGATCTT+TGG | 0.189029 | 7.1:-13950388 | MS.gene018017:CDS |
| AATAATATTGATGGATCTTT+GGG | 0.232329 | 7.1:-13950387 | MS.gene018017:CDS |
| AAACTGCACAAGATCTTTCT+TGG | 0.245892 | 7.1:-13950668 | MS.gene018017:CDS |
| CGACCTTGATTTGAAGCTTT+AGG | 0.290526 | 7.1:-13950265 | None:intergenic |
| ACACAATTTGCAACATAAAT+CGG | 0.316739 | 7.1:-13950632 | MS.gene018017:CDS |
| CATGATTACAATAATATTGA+TGG | 0.344856 | 7.1:-13950396 | MS.gene018017:CDS |
| ACCGCTGGTAATTCTTCAAT+TGG | 0.376621 | 7.1:+13950315 | None:intergenic |
| TGTAAATCTTGTGATGTTGT+TGG | 0.389716 | 7.1:+13950567 | None:intergenic |
| CACAATTTGCAACATAAATC+GGG | 0.419218 | 7.1:-13950631 | MS.gene018017:CDS |
| TGTGATGAAGGTGGTGGAGA+TGG | 0.423532 | 7.1:+13950516 | None:intergenic |
| ACAATTGGAACCTCTGGTAT+AGG | 0.433089 | 7.1:+13950432 | None:intergenic |
| TGCAAATTGTGTATGAGCTT+TGG | 0.452975 | 7.1:+13950643 | None:intergenic |
| CAACCTAAAGCTTCAAATCA+AGG | 0.460309 | 7.1:+13950262 | None:intergenic |
| TGACGAGTACGAGAGACAAT+TGG | 0.479925 | 7.1:+13950417 | None:intergenic |
| CGAGAGACAATTGGAACCTC+TGG | 0.486624 | 7.1:+13950426 | None:intergenic |
| AAGAATTACCAGCGGTCTGG+TGG | 0.491691 | 7.1:-13950308 | MS.gene018017:CDS |
| TCAATTGGAGCAGAATCAAT+CGG | 0.499372 | 7.1:+13950330 | None:intergenic |
| TTGAAGAATTACCAGCGGTC+TGG | 0.500257 | 7.1:-13950311 | MS.gene018017:CDS |
| ACCATGTTGTTTCCTATGCA+TGG | 0.506943 | 7.1:-13950492 | MS.gene018017:CDS |
| AACCTCTGGTATAGGCCTCA+TGG | 0.524083 | 7.1:+13950440 | None:intergenic |
| TTACCAGCGGTCTGGTGGGA+TGG | 0.524380 | 7.1:-13950303 | MS.gene018017:CDS |
| GTTGGTATGAATGTTTGTGA+TGG | 0.529752 | 7.1:+13950585 | None:intergenic |
| TTTCCATCCCACCAGACCGC+TGG | 0.540570 | 7.1:+13950300 | None:intergenic |
| AGATCTTCTTTGTACCACAT+TGG | 0.545928 | 7.1:-13950543 | MS.gene018017:CDS |
| AACAACATGGTGTGTGATGA+AGG | 0.556950 | 7.1:+13950504 | None:intergenic |
| TGGTTGCATCGAAATTCGAT+TGG | 0.568245 | 7.1:+13950708 | None:intergenic |
| TGGATCTTTGGGGAGAACAA+TGG | 0.571321 | 7.1:-13950376 | MS.gene018017:CDS |
| TTGTTTCCTATGCATGGAAG+TGG | 0.582090 | 7.1:-13950486 | MS.gene018017:CDS |
| TAGGCCTCATGGATGGATGA+AGG | 0.586352 | 7.1:+13950451 | None:intergenic |
| TAATTACCACTTCCATGCAT+AGG | 0.590506 | 7.1:+13950480 | None:intergenic |
| GTTAGAGTAAGAACAAGAGT+AGG | 0.594186 | 7.1:+13950732 | None:intergenic |
| GATGAAGGTGGTGGAGATGG+TGG | 0.599841 | 7.1:+13950519 | None:intergenic |
| TCTGGTATAGGCCTCATGGA+TGG | 0.600026 | 7.1:+13950444 | None:intergenic |
| GTGGAGATGGTGGTCCAATG+TGG | 0.620516 | 7.1:+13950529 | None:intergenic |
| ATCCATGAGGCCTATACCAG+AGG | 0.624967 | 7.1:-13950442 | MS.gene018017:CDS |
| AGAATTACCAGCGGTCTGGT+GGG | 0.635470 | 7.1:-13950307 | MS.gene018017:CDS |
| ATAATATTGATGGATCTTTG+GGG | 0.673989 | 7.1:-13950386 | MS.gene018017:CDS |
| ATGGTGTGTGATGAAGGTGG+TGG | 0.684137 | 7.1:+13950510 | None:intergenic |
| TCCAATTGAAGAATTACCAG+CGG | 0.686028 | 7.1:-13950316 | MS.gene018017:CDS |
| GCCTCATGGATGGATGAAGG+TGG | 0.701559 | 7.1:+13950454 | None:intergenic |
| ACCACCTTCATCCATCCATG+AGG | 0.719012 | 7.1:-13950455 | MS.gene018017:CDS |
| TCCATGCATAGGAAACAACA+TGG | 0.729158 | 7.1:+13950491 | None:intergenic |
| AACATGGTGTGTGATGAAGG+TGG | 0.787924 | 7.1:+13950507 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CATGATTACAATAATATTGA+TGG | - | chr7.1:13950674-13950693 | MS.gene018017:CDS | 20.0% |
| !!! | AATAATATTGATGGATCTTT+GGG | - | chr7.1:13950683-13950702 | MS.gene018017:CDS | 20.0% |
| ! | ACACAATTTGCAACATAAAT+CGG | - | chr7.1:13950438-13950457 | MS.gene018017:CDS | 25.0% |
| !! | ATAATATTGATGGATCTTTG+GGG | - | chr7.1:13950684-13950703 | MS.gene018017:CDS | 25.0% |
| !! | CAATAATATTGATGGATCTT+TGG | - | chr7.1:13950682-13950701 | MS.gene018017:CDS | 25.0% |
| CACAATTTGCAACATAAATC+GGG | - | chr7.1:13950439-13950458 | MS.gene018017:CDS | 30.0% | |
| TGTAAATCTTGTGATGTTGT+TGG | + | chr7.1:13950506-13950525 | None:intergenic | 30.0% | |
| AAACTGCACAAGATCTTTCT+TGG | - | chr7.1:13950402-13950421 | MS.gene018017:CDS | 35.0% | |
| AGATCTTCTTTGTACCACAT+TGG | - | chr7.1:13950527-13950546 | MS.gene018017:CDS | 35.0% | |
| GTTAGAGTAAGAACAAGAGT+AGG | + | chr7.1:13950341-13950360 | None:intergenic | 35.0% | |
| GTTGGTATGAATGTTTGTGA+TGG | + | chr7.1:13950488-13950507 | None:intergenic | 35.0% | |
| TAATTACCACTTCCATGCAT+AGG | + | chr7.1:13950593-13950612 | None:intergenic | 35.0% | |
| TCAATTGGAGCAGAATCAAT+CGG | + | chr7.1:13950743-13950762 | None:intergenic | 35.0% | |
| TCCAATTGAAGAATTACCAG+CGG | - | chr7.1:13950754-13950773 | MS.gene018017:CDS | 35.0% | |
| TGCAAATTGTGTATGAGCTT+TGG | + | chr7.1:13950430-13950449 | None:intergenic | 35.0% | |
| !! | AAGATCTTGTGCAGTTTTGA+AGG | + | chr7.1:13950399-13950418 | None:intergenic | 35.0% |
| !!! | TTTTGAAGGTGTTATGGCAA+TGG | + | chr7.1:13950385-13950404 | None:intergenic | 35.0% |
| AACAACATGGTGTGTGATGA+AGG | + | chr7.1:13950569-13950588 | None:intergenic | 40.0% | |
| ACAATTGGAACCTCTGGTAT+AGG | + | chr7.1:13950641-13950660 | None:intergenic | 40.0% | |
| ACCGCTGGTAATTCTTCAAT+TGG | + | chr7.1:13950758-13950777 | None:intergenic | 40.0% | |
| TCCATGCATAGGAAACAACA+TGG | + | chr7.1:13950582-13950601 | None:intergenic | 40.0% | |
| TGGTTGCATCGAAATTCGAT+TGG | + | chr7.1:13950365-13950384 | None:intergenic | 40.0% | |
| TTGTTTCCTATGCATGGAAG+TGG | - | chr7.1:13950584-13950603 | MS.gene018017:CDS | 40.0% | |
| !! | ACCATGTTGTTTCCTATGCA+TGG | - | chr7.1:13950578-13950597 | MS.gene018017:CDS | 40.0% |
| !!! | GTGCAGTTTTGAAGGTGTTA+TGG | + | chr7.1:13950391-13950410 | None:intergenic | 40.0% |
| AACATGGTGTGTGATGAAGG+TGG | + | chr7.1:13950566-13950585 | None:intergenic | 45.0% | |
| TGACGAGTACGAGAGACAAT+TGG | + | chr7.1:13950656-13950675 | None:intergenic | 45.0% | |
| TTGAAGAATTACCAGCGGTC+TGG | - | chr7.1:13950759-13950778 | MS.gene018017:CDS | 45.0% | |
| ! | TGGATCTTTGGGGAGAACAA+TGG | - | chr7.1:13950694-13950713 | MS.gene018017:CDS | 45.0% |
| AACCTCTGGTATAGGCCTCA+TGG | + | chr7.1:13950633-13950652 | None:intergenic | 50.0% | |
| AAGAATTACCAGCGGTCTGG+TGG | - | chr7.1:13950762-13950781 | MS.gene018017:CDS | 50.0% | |
| ACCACCTTCATCCATCCATG+AGG | - | chr7.1:13950615-13950634 | MS.gene018017:CDS | 50.0% | |
| AGAATTACCAGCGGTCTGGT+GGG | - | chr7.1:13950763-13950782 | MS.gene018017:CDS | 50.0% | |
| ATCCATGAGGCCTATACCAG+AGG | - | chr7.1:13950628-13950647 | MS.gene018017:CDS | 50.0% | |
| ATGGTGTGTGATGAAGGTGG+TGG | + | chr7.1:13950563-13950582 | None:intergenic | 50.0% | |
| CGAGAGACAATTGGAACCTC+TGG | + | chr7.1:13950647-13950666 | None:intergenic | 50.0% | |
| TAGGCCTCATGGATGGATGA+AGG | + | chr7.1:13950622-13950641 | None:intergenic | 50.0% | |
| TCTGGTATAGGCCTCATGGA+TGG | + | chr7.1:13950629-13950648 | None:intergenic | 50.0% | |
| !! | TGTGATGAAGGTGGTGGAGA+TGG | + | chr7.1:13950557-13950576 | None:intergenic | 50.0% |
| GCCTCATGGATGGATGAAGG+TGG | + | chr7.1:13950619-13950638 | None:intergenic | 55.0% | |
| ! | GTGGAGATGGTGGTCCAATG+TGG | + | chr7.1:13950544-13950563 | None:intergenic | 55.0% |
| !! | GATGAAGGTGGTGGAGATGG+TGG | + | chr7.1:13950554-13950573 | None:intergenic | 55.0% |
| TTACCAGCGGTCTGGTGGGA+TGG | - | chr7.1:13950767-13950786 | MS.gene018017:CDS | 60.0% | |
| TTTCCATCCCACCAGACCGC+TGG | + | chr7.1:13950773-13950792 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 13950266 | 13950826 | 13950266 | ID=MS.gene018017 |
| chr7.1 | mRNA | 13950266 | 13950826 | 13950266 | ID=MS.gene018017.t1;Parent=MS.gene018017 |
| chr7.1 | exon | 13950266 | 13950826 | 13950266 | ID=MS.gene018017.t1.exon1;Parent=MS.gene018017.t1 |
| chr7.1 | CDS | 13950266 | 13950826 | 13950266 | ID=cds.MS.gene018017.t1;Parent=MS.gene018017.t1 |
| Gene Sequence |
| Protein sequence |
