Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018540.t1 | XP_013450702.1 | 70.2 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 4.50E-48 | 200.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018540.t1 | O23659 | 35.4 | 113 | 65 | 2 | 24 | 128 | 199 | 311 | 2.4e-13 | 76.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018540.t1 | A0A072UG20 | 70.2 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 3.3e-48 | 200.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018540.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018540.t1 | MTR_6g004120 | 70.229 | 131 | 39 | 0 | 1 | 131 | 1 | 131 | 3.51e-65 | 195 |
| MS.gene018540.t1 | MTR_1g040600 | 45.695 | 151 | 55 | 4 | 3 | 128 | 5 | 153 | 4.83e-32 | 111 |
| MS.gene018540.t1 | MTR_1g045700 | 50.893 | 112 | 51 | 3 | 23 | 130 | 111 | 222 | 9.43e-30 | 107 |
| MS.gene018540.t1 | MTR_1g046880 | 38.889 | 162 | 62 | 5 | 5 | 129 | 9 | 170 | 1.78e-26 | 97.8 |
| MS.gene018540.t1 | MTR_2g058520 | 44.348 | 115 | 56 | 2 | 23 | 129 | 62 | 176 | 1.65e-25 | 95.5 |
| MS.gene018540.t1 | MTR_1g046720 | 43.966 | 116 | 58 | 3 | 21 | 129 | 2 | 117 | 2.52e-25 | 93.2 |
| MS.gene018540.t1 | MTR_1g046550 | 45.763 | 118 | 58 | 4 | 18 | 129 | 145 | 262 | 3.31e-25 | 96.7 |
| MS.gene018540.t1 | MTR_1g045740 | 41.322 | 121 | 63 | 3 | 22 | 134 | 144 | 264 | 3.61e-24 | 94.4 |
| MS.gene018540.t1 | MTR_1g045720 | 43.966 | 116 | 57 | 3 | 22 | 129 | 144 | 259 | 6.93e-24 | 93.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018540.t1 | AT1G78640 | 41.228 | 114 | 58 | 2 | 25 | 129 | 154 | 267 | 1.50e-19 | 82.0 |
| MS.gene018540.t1 | AT2G33720 | 35.398 | 113 | 65 | 2 | 24 | 128 | 199 | 311 | 2.03e-16 | 74.3 |
| MS.gene018540.t1 | AT1G78635 | 37.705 | 122 | 65 | 4 | 26 | 139 | 121 | 239 | 5.51e-15 | 69.3 |
| MS.gene018540.t1 | AT4G02870 | 32.759 | 116 | 62 | 4 | 26 | 131 | 191 | 300 | 3.40e-11 | 59.7 |
Find 22 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGATAAGATTGGTCTTTAT+TGG | 0.198682 | 7.4:-10015227 | MS.gene018540:CDS |
| TAGGAATTCGAGCTGAAATA+TGG | 0.273058 | 7.4:-10015362 | MS.gene018540:CDS |
| GGATAAGATTGGTCTTTATT+GGG | 0.344915 | 7.4:-10015226 | MS.gene018540:CDS |
| CTCAAATTCAAGAGATTGTC+TGG | 0.348648 | 7.4:-10015312 | MS.gene018540:CDS |
| GAACAGTAAAATTGAAGAAT+CGG | 0.427975 | 7.4:+10015193 | None:intergenic |
| GACACACTCCTAGAGATAAA+TGG | 0.428020 | 7.4:+10015523 | None:intergenic |
| GTTTGTGACCATTTATCTCT+AGG | 0.448904 | 7.4:-10015531 | MS.gene018540:CDS |
| TTGGGTGGTAATGTTGAGAT+AGG | 0.471139 | 7.4:-10015381 | MS.gene018540:CDS |
| AGGAATTCGAGCTGAAATAT+GGG | 0.489554 | 7.4:-10015361 | MS.gene018540:CDS |
| GTACAAGCAACGATGACTCT+TGG | 0.507283 | 7.4:-10015497 | MS.gene018540:CDS |
| TTATGGCTTATAGCAACAGT+AGG | 0.507490 | 7.4:-10015554 | MS.gene018540:CDS |
| CTTGAATTTGAGAACGTGCA+TGG | 0.514512 | 7.4:+10015323 | None:intergenic |
| GAGTCATCGTTGCTTGTACA+AGG | 0.516173 | 7.4:+10015501 | None:intergenic |
| TTAAAGCAAGGGGATAAGAT+TGG | 0.521643 | 7.4:-10015237 | MS.gene018540:CDS |
| GTGTTGACAATGAGTGATAC+CGG | 0.535346 | 7.4:-10015462 | MS.gene018540:CDS |
| CCAACAAAAGTCTTCTCATA+CGG | 0.561709 | 7.4:+10015439 | None:intergenic |
| AAAGAAGAGGCTTAAAGCAA+GGG | 0.562567 | 7.4:-10015248 | MS.gene018540:CDS |
| AAAAGAAGAGGCTTAAAGCA+AGG | 0.562720 | 7.4:-10015249 | MS.gene018540:CDS |
| TTTGTGTTGTCTGTGTTGGG+TGG | 0.569410 | 7.4:-10015396 | MS.gene018540:CDS |
| TATGGCTTATAGCAACAGTA+GGG | 0.575545 | 7.4:-10015553 | MS.gene018540:CDS |
| CAAAAGTCTTCTCATACGGC+CGG | 0.580656 | 7.4:+10015443 | None:intergenic |
| AAGAAGAGGCTTAAAGCAAG+GGG | 0.637026 | 7.4:-10015247 | MS.gene018540:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTTGGAATAATAAAAT+TGG | + | chr7.4:10015546-10015565 | None:intergenic | 10.0% |
| ! | GAACAGTAAAATTGAAGAAT+CGG | + | chr7.4:10015517-10015536 | None:intergenic | 25.0% |
| !! | ACTTTTGTTGGGTAAAAAAT+TGG | - | chr7.4:10015280-10015299 | MS.gene018540:CDS | 25.0% |
| GACAAGTTTGTAAAAAGAAG+AGG | - | chr7.4:10015446-10015465 | MS.gene018540:CDS | 30.0% | |
| ! | ATATATTTTCACGGATGGTT+GGG | - | chr7.4:10015421-10015440 | MS.gene018540:CDS | 30.0% |
| ! | CTTCAATTTTACTGTTCTTG+AGG | - | chr7.4:10015520-10015539 | MS.gene018540:CDS | 30.0% |
| ! | GTCTGGATCATATATTTTCA+CGG | - | chr7.4:10015412-10015431 | MS.gene018540:CDS | 30.0% |
| !! | GGATAAGATTGGTCTTTATT+GGG | - | chr7.4:10015481-10015500 | MS.gene018540:CDS | 30.0% |
| AAAAGAAGAGGCTTAAAGCA+AGG | - | chr7.4:10015458-10015477 | MS.gene018540:CDS | 35.0% | |
| AAAGAAGAGGCTTAAAGCAA+GGG | - | chr7.4:10015459-10015478 | MS.gene018540:CDS | 35.0% | |
| AGGAATTCGAGCTGAAATAT+GGG | - | chr7.4:10015346-10015365 | MS.gene018540:CDS | 35.0% | |
| CCAACAAAAGTCTTCTCATA+CGG | + | chr7.4:10015271-10015290 | None:intergenic | 35.0% | |
| CTCAAATTCAAGAGATTGTC+TGG | - | chr7.4:10015395-10015414 | MS.gene018540:CDS | 35.0% | |
| GTTTGTGACCATTTATCTCT+AGG | - | chr7.4:10015176-10015195 | MS.gene018540:CDS | 35.0% | |
| TAGGAATTCGAGCTGAAATA+TGG | - | chr7.4:10015345-10015364 | MS.gene018540:CDS | 35.0% | |
| TTAAAGCAAGGGGATAAGAT+TGG | - | chr7.4:10015470-10015489 | MS.gene018540:CDS | 35.0% | |
| ! | CATATATTTTCACGGATGGT+TGG | - | chr7.4:10015420-10015439 | MS.gene018540:CDS | 35.0% |
| ! | CGTATGAGAAGACTTTTGTT+GGG | - | chr7.4:10015269-10015288 | MS.gene018540:CDS | 35.0% |
| ! | GATTTTGTGTTGTCTGTGTT+GGG | - | chr7.4:10015308-10015327 | MS.gene018540:CDS | 35.0% |
| ! | GGATCATATATTTTCACGGA+TGG | - | chr7.4:10015416-10015435 | MS.gene018540:CDS | 35.0% |
| !! | GGGATAAGATTGGTCTTTAT+TGG | - | chr7.4:10015480-10015499 | MS.gene018540:CDS | 35.0% |
| AAGAAGAGGCTTAAAGCAAG+GGG | - | chr7.4:10015460-10015479 | MS.gene018540:CDS | 40.0% | |
| CTTGAATTTGAGAACGTGCA+TGG | + | chr7.4:10015387-10015406 | None:intergenic | 40.0% | |
| GACACACTCCTAGAGATAAA+TGG | + | chr7.4:10015187-10015206 | None:intergenic | 40.0% | |
| GTTGGGTAAAAAATTGGCTG+AGG | - | chr7.4:10015286-10015305 | MS.gene018540:CDS | 40.0% | |
| ! | CCGTATGAGAAGACTTTTGT+TGG | - | chr7.4:10015268-10015287 | MS.gene018540:CDS | 40.0% |
| ! | GGATTTTGTGTTGTCTGTGT+TGG | - | chr7.4:10015307-10015326 | MS.gene018540:CDS | 40.0% |
| ! | GTGTTGACAATGAGTGATAC+CGG | - | chr7.4:10015245-10015264 | MS.gene018540:CDS | 40.0% |
| ! | TTGGGTGGTAATGTTGAGAT+AGG | - | chr7.4:10015326-10015345 | MS.gene018540:CDS | 40.0% |
| CAAAAGTCTTCTCATACGGC+CGG | + | chr7.4:10015267-10015286 | None:intergenic | 45.0% | |
| GAGTCATCGTTGCTTGTACA+AGG | + | chr7.4:10015209-10015228 | None:intergenic | 45.0% | |
| GTACAAGCAACGATGACTCT+TGG | - | chr7.4:10015210-10015229 | MS.gene018540:CDS | 45.0% | |
| TTTGTGTTGTCTGTGTTGGG+TGG | - | chr7.4:10015311-10015330 | MS.gene018540:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10015155 | 10015574 | 10015155 | ID=MS.gene018540 |
| chr7.4 | mRNA | 10015155 | 10015574 | 10015155 | ID=MS.gene018540.t1;Parent=MS.gene018540 |
| chr7.4 | exon | 10015155 | 10015574 | 10015155 | ID=MS.gene018540.t1.exon1;Parent=MS.gene018540.t1 |
| chr7.4 | CDS | 10015155 | 10015574 | 10015155 | ID=cds.MS.gene018540.t1;Parent=MS.gene018540.t1 |
| Gene Sequence |
| Protein sequence |
