Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018549.t1 | GAU37750.1 | 39.3 | 183 | 109 | 2 | 16 | 196 | 27 | 209 | 7.90E-30 | 140.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018549.t1 | A0A2Z6NMK1 | 39.3 | 183 | 109 | 2 | 16 | 196 | 27 | 209 | 5.7e-30 | 140.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018549.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018549.t1 | MTR_4g123470 | 29.560 | 159 | 112 | 0 | 23 | 181 | 52 | 210 | 1.64e-21 | 88.2 |
| MS.gene018549.t1 | MTR_1g054495 | 23.494 | 166 | 126 | 1 | 31 | 196 | 57 | 221 | 1.23e-12 | 64.7 |
| MS.gene018549.t1 | MTR_0014s0220 | 28.472 | 144 | 87 | 3 | 39 | 182 | 683 | 810 | 1.25e-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 7.4:+10250392 | MS.gene018549:CDS |
| AATGATCTTTATGAAGTTCT+TGG | 0.265359 | 7.4:+10250059 | MS.gene018549:CDS |
| CAAATGTAATGAAGGTTTCT+AGG | 0.290265 | 7.4:+10250527 | MS.gene018549:CDS |
| TCATAACCTGCAGCCATATA+AGG | 0.313221 | 7.4:-10250201 | None:intergenic |
| ATGATCTTTATGAAGTTCTT+GGG | 0.324200 | 7.4:+10250060 | MS.gene018549:CDS |
| AATGATCTTAACTCCTTATA+TGG | 0.327626 | 7.4:+10250188 | MS.gene018549:CDS |
| CTGAGGGATGGATCAACTTA+AGG | 0.360735 | 7.4:+10250010 | MS.gene018549:CDS |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 7.4:-10250095 | None:intergenic |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 7.4:+10250492 | MS.gene018549:CDS |
| TATGGCTGCAGGTTATGAAT+TGG | 0.411365 | 7.4:+10250206 | MS.gene018549:CDS |
| GAGTTAAGATCATTTCATAA+TGG | 0.420070 | 7.4:-10250179 | None:intergenic |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 7.4:+10250328 | MS.gene018549:CDS |
| TTCCAATTCACTGCATTTCG+TGG | 0.427022 | 7.4:+10250093 | MS.gene018549:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 7.4:+10250307 | MS.gene018549:intron |
| TTCTCTGCTATTAATATGTA+TGG | 0.435001 | 7.4:+10250352 | MS.gene018549:CDS |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 7.4:+10250393 | MS.gene018549:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.444993 | 7.4:+10250011 | MS.gene018549:CDS |
| TTAACTCCTTATATGGCTGC+AGG | 0.483063 | 7.4:+10250195 | MS.gene018549:CDS |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 7.4:+10250361 | MS.gene018549:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 7.4:+10250463 | MS.gene018549:CDS |
| CAACAAAGATTGGCAGTGGA+TGG | 0.512212 | 7.4:+10250431 | MS.gene018549:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 7.4:+10250149 | MS.gene018549:CDS |
| AAGTACATCAAATGTAATGA+AGG | 0.524181 | 7.4:+10250519 | MS.gene018549:CDS |
| ATGTCAACTCATTGCCATCA+TGG | 0.539340 | 7.4:+10250124 | MS.gene018549:CDS |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 7.4:+10250467 | MS.gene018549:CDS |
| ATCCATCCCTCAGTTAGAAG+TGG | 0.556411 | 7.4:-10250000 | None:intergenic |
| ATAAAATGCTCAACAAAGGA+AGG | 0.558305 | 7.4:-10249897 | None:intergenic |
| TGATCTTTATGAAGTTCTTG+GGG | 0.561506 | 7.4:+10250061 | MS.gene018549:CDS |
| AAGGCAACAAAGATTGGCAG+TGG | 0.562007 | 7.4:+10250427 | MS.gene018549:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 7.4:-10250138 | None:intergenic |
| ATCCACTTCTAACTGAGGGA+TGG | 0.574184 | 7.4:+10249998 | MS.gene018549:CDS |
| GTTGATAAAATGCTCAACAA+AGG | 0.587741 | 7.4:-10249901 | None:intergenic |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 7.4:-10250376 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 7.4:-10250386 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 7.4:+10250397 | MS.gene018549:CDS |
| TGAACATCCACTTCTAACTG+AGG | 0.624857 | 7.4:+10249993 | MS.gene018549:CDS |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 7.4:-10250480 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 7.4:+10250466 | MS.gene018549:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 7.4:+10250468 | MS.gene018549:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 7.4:+10250141 | MS.gene018549:CDS |
| GAACATCCACTTCTAACTGA+GGG | 0.652020 | 7.4:+10249994 | MS.gene018549:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AACAAATTTTGTAAAGACAA+TGG | + | chr7.4:10250454-10250473 | MS.gene018549:CDS | 20.0% |
| ! | AAGTACATCAAATGTAATGA+AGG | + | chr7.4:10250519-10250538 | MS.gene018549:CDS | 25.0% |
| ! | AATGATCTTAACTCCTTATA+TGG | + | chr7.4:10250188-10250207 | MS.gene018549:CDS | 25.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | + | chr7.4:10250059-10250078 | MS.gene018549:CDS | 25.0% |
| ! | ACAAATGTGATGTACTAATA+AGG | + | chr7.4:10250392-10250411 | MS.gene018549:CDS | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | + | chr7.4:10250060-10250079 | MS.gene018549:CDS | 25.0% |
| ! | CAAATGTGATGTACTAATAA+GGG | + | chr7.4:10250393-10250412 | MS.gene018549:CDS | 25.0% |
| ! | GAGTTAAGATCATTTCATAA+TGG | - | chr7.4:10250182-10250201 | None:intergenic | 25.0% |
| ! | TTCTCTGCTATTAATATGTA+TGG | + | chr7.4:10250352-10250371 | MS.gene018549:CDS | 25.0% |
| !! | GAACTTAACTTTTTGTCTAT+TGG | - | chr7.4:10249962-10249981 | None:intergenic | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | + | chr7.4:10250492-10250511 | MS.gene018549:CDS | 25.0% |
| !! | TAGAGATGTATCATTTTAGA+AGG | - | chr7.4:10250247-10250266 | None:intergenic | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | + | chr7.4:10250421-10250440 | MS.gene018549:CDS | 30.0% | |
| AATAAGGGATGGAAAAAAGA+AGG | + | chr7.4:10250408-10250427 | MS.gene018549:CDS | 30.0% | |
| ATAAAATGCTCAACAAAGGA+AGG | - | chr7.4:10249900-10249919 | None:intergenic | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | + | chr7.4:10250361-10250380 | MS.gene018549:CDS | 30.0% | |
| CAAATGTAATGAAGGTTTCT+AGG | + | chr7.4:10250527-10250546 | MS.gene018549:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | - | chr7.4:10250389-10250408 | None:intergenic | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | + | chr7.4:10250061-10250080 | MS.gene018549:CDS | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | + | chr7.4:10250021-10250040 | MS.gene018549:CDS | 30.0% |
| ! | GTTGATAAAATGCTCAACAA+AGG | - | chr7.4:10249904-10249923 | None:intergenic | 30.0% |
| !! | ATCATTTTAGAAGGAGTTGT+TGG | - | chr7.4:10250238-10250257 | None:intergenic | 30.0% |
| TGTGATGTACTAATAAGGGA+TGG | + | chr7.4:10250397-10250416 | MS.gene018549:CDS | 35.0% | |
| ! | TGTAAAGACAATGGATTCCA+TGG | + | chr7.4:10250463-10250482 | MS.gene018549:CDS | 35.0% |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | + | chr7.4:10250025-10250044 | MS.gene018549:CDS | 35.0% |
| ATGTCAACTCATTGCCATCA+TGG | + | chr7.4:10250124-10250143 | MS.gene018549:CDS | 40.0% | |
| CACTTACAAGCAAGGAATTG+AGG | + | chr7.4:10250149-10250168 | MS.gene018549:CDS | 40.0% | |
| GAACATCCACTTCTAACTGA+GGG | + | chr7.4:10249994-10250013 | MS.gene018549:CDS | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | + | chr7.4:10250328-10250347 | MS.gene018549:CDS | 40.0% | |
| TATGGCTGCAGGTTATGAAT+TGG | + | chr7.4:10250206-10250225 | MS.gene018549:CDS | 40.0% | |
| TCATAACCTGCAGCCATATA+AGG | - | chr7.4:10250204-10250223 | None:intergenic | 40.0% | |
| TGAACATCCACTTCTAACTG+AGG | + | chr7.4:10249993-10250012 | MS.gene018549:CDS | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | + | chr7.4:10250011-10250030 | MS.gene018549:CDS | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | + | chr7.4:10250195-10250214 | MS.gene018549:CDS | 40.0% | |
| TTCCAATTCACTGCATTTCG+TGG | + | chr7.4:10250093-10250112 | MS.gene018549:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | + | chr7.4:10250466-10250485 | MS.gene018549:CDS | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | + | chr7.4:10250467-10250486 | MS.gene018549:CDS | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | + | chr7.4:10250307-10250326 | MS.gene018549:intron | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | + | chr7.4:10250427-10250446 | MS.gene018549:CDS | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | - | chr7.4:10250483-10250502 | None:intergenic | 45.0% | |
| ATCCACTTCTAACTGAGGGA+TGG | + | chr7.4:10249998-10250017 | MS.gene018549:CDS | 45.0% | |
| ATCCATCCCTCAGTTAGAAG+TGG | - | chr7.4:10250003-10250022 | None:intergenic | 45.0% | |
| CAACAAAGATTGGCAGTGGA+TGG | + | chr7.4:10250431-10250450 | MS.gene018549:CDS | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | + | chr7.4:10250010-10250029 | MS.gene018549:CDS | 45.0% | |
| GTCCACGAAATGCAGTGAAT+TGG | - | chr7.4:10250098-10250117 | None:intergenic | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | + | chr7.4:10250141-10250160 | MS.gene018549:CDS | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | - | chr7.4:10250141-10250160 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | + | chr7.4:10250468-10250487 | MS.gene018549:CDS | 45.0% |
| !! | CATTTGTACTCGGTGCCTGT+AGG | - | chr7.4:10250379-10250398 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 10249883 | 10250555 | 10249883 | ID=MS.gene018549 |
| chr7.4 | mRNA | 10249883 | 10250555 | 10249883 | ID=MS.gene018549.t1;Parent=MS.gene018549 |
| chr7.4 | exon | 10249883 | 10250227 | 10249883 | ID=MS.gene018549.t1.exon1;Parent=MS.gene018549.t1 |
| chr7.4 | CDS | 10249883 | 10250227 | 10249883 | ID=cds.MS.gene018549.t1;Parent=MS.gene018549.t1 |
| chr7.4 | exon | 10250310 | 10250555 | 10250310 | ID=MS.gene018549.t1.exon2;Parent=MS.gene018549.t1 |
| chr7.4 | CDS | 10250310 | 10250555 | 10250310 | ID=cds.MS.gene018549.t1;Parent=MS.gene018549.t1 |
| Gene Sequence |
| Protein sequence |
