Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018614.t1 | XP_003625416.1 | 94.3 | 140 | 6 | 1 | 1 | 138 | 1 | 140 | 2.00E-64 | 255 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018614.t1 | F4JZT3 | 59.2 | 120 | 48 | 1 | 13 | 131 | 11 | 130 | 4.6e-33 | 142.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018614.t1 | G7KYH3 | 94.3 | 140 | 6 | 1 | 1 | 138 | 1 | 140 | 1.4e-64 | 255.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018614.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018614.t1 | MTR_7g098910 | 94.286 | 140 | 6 | 1 | 1 | 138 | 1 | 140 | 1.86e-91 | 261 |
| MS.gene018614.t1 | MTR_7g099500 | 60.317 | 126 | 49 | 1 | 13 | 137 | 4 | 129 | 1.57e-47 | 150 |
| MS.gene018614.t1 | MTR_8g063580 | 58.594 | 128 | 50 | 1 | 14 | 138 | 6 | 133 | 5.20e-44 | 141 |
| MS.gene018614.t1 | MTR_0022s0450 | 77.049 | 61 | 14 | 0 | 26 | 86 | 40 | 100 | 1.42e-26 | 95.9 |
| MS.gene018614.t1 | MTR_7g098860 | 48.276 | 87 | 40 | 3 | 43 | 124 | 12 | 98 | 1.15e-16 | 70.5 |
| MS.gene018614.t1 | MTR_6g008860 | 34.286 | 105 | 68 | 1 | 20 | 123 | 33 | 137 | 7.44e-16 | 69.7 |
| MS.gene018614.t1 | MTR_4g071650 | 34.286 | 105 | 68 | 1 | 20 | 123 | 33 | 137 | 7.44e-16 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018614.t1 | AT5G26594 | 59.167 | 120 | 48 | 1 | 13 | 131 | 11 | 130 | 7.06e-45 | 143 |
| MS.gene018614.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.13e-29 | 103 |
| MS.gene018614.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.13e-29 | 103 |
| MS.gene018614.t1 | AT3G04280 | 47.934 | 121 | 56 | 3 | 20 | 133 | 22 | 142 | 6.13e-29 | 103 |
Find 29 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAGAAAAGCAAGAATTTA+TGG | 0.203520 | 7.3:+13362787 | MS.gene018614:CDS |
| ATAATGGAACCTCAAAATTT+AGG | 0.298816 | 7.3:+13362329 | MS.gene018614:CDS |
| TAACATCATTCTCATGGATA+AGG | 0.382325 | 7.3:+13362496 | MS.gene018614:CDS |
| AGGGAAAGGGAAAGGGATAA+TGG | 0.389184 | 7.3:+13362313 | MS.gene018614:CDS |
| ACCCATCGATCGCAGTTTCT+TGG | 0.391435 | 7.3:-13362726 | None:intergenic |
| AAGGAAATGCCTATCATGAA+TGG | 0.455972 | 7.3:+13362515 | MS.gene018614:CDS |
| AAATGGAAGATCAAGGGAAA+GGG | 0.458192 | 7.3:+13362300 | None:intergenic |
| AAGCAAGAATTTATGGAAGC+AGG | 0.462808 | 7.3:+13362794 | MS.gene018614:CDS |
| AAGATTTAACATCATTCTCA+TGG | 0.473978 | 7.3:+13362490 | MS.gene018614:CDS |
| ACCTCAATCCCATTCATGAT+AGG | 0.485829 | 7.3:-13362524 | None:intergenic |
| TTAAGCACACCAATGGTCAA+TGG | 0.495806 | 7.3:-13362839 | None:intergenic |
| TTGTACAAAATGGAAGATCA+AGG | 0.500428 | 7.3:+13362293 | None:intergenic |
| AAGATCAAGGGAAAGGGAAA+GGG | 0.503589 | 7.3:+13362306 | MS.gene018614:CDS |
| AGGAAATGCCTATCATGAAT+GGG | 0.507526 | 7.3:+13362516 | MS.gene018614:CDS |
| TAAATCTTTGACCATTGCAA+TGG | 0.518192 | 7.3:-13362475 | None:intergenic |
| AGCAAGAATTTATGGAAGCA+GGG | 0.519897 | 7.3:+13362795 | MS.gene018614:CDS |
| GCCTATCATGAATGGGATTG+AGG | 0.520152 | 7.3:+13362523 | MS.gene018614:CDS |
| GAAGATCAAGGGAAAGGGAA+AGG | 0.522711 | 7.3:+13362305 | MS.gene018614:CDS |
| AACCAAGAAACTGCGATCGA+TGG | 0.533042 | 7.3:+13362724 | MS.gene018614:CDS |
| CTTGTTCGAATGATTCATCA+AGG | 0.541897 | 7.3:+13362389 | MS.gene018614:CDS |
| CAAGGTCTATTGAAAAGAGC+TGG | 0.545224 | 7.3:+13362407 | MS.gene018614:CDS |
| ACCAAGAAACTGCGATCGAT+GGG | 0.549417 | 7.3:+13362725 | MS.gene018614:CDS |
| CAAGTGAAACCATTGACCAT+TGG | 0.555088 | 7.3:+13362830 | MS.gene018614:CDS |
| GGTATCCGTAGCATGATTGT+TGG | 0.563539 | 7.3:+13362746 | MS.gene018614:CDS |
| AAAATGGAAGATCAAGGGAA+AGG | 0.568166 | 7.3:+13362299 | None:intergenic |
| ACACACCAACAATCATGCTA+CGG | 0.608934 | 7.3:-13362751 | None:intergenic |
| TGTACAAAATGGAAGATCAA+GGG | 0.616219 | 7.3:+13362294 | None:intergenic |
| AATCGAATTAAGCACACCAA+TGG | 0.617947 | 7.3:-13362846 | None:intergenic |
| GCTGTAGATATCCATTGCAA+TGG | 0.620859 | 7.3:+13362464 | MS.gene018614:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATACATAACAAAGTTAAT+AGG | - | chr7.3:13362618-13362637 | None:intergenic | 15.0% |
| !!! | ATTTTAAATATAATTGCACA+TGG | - | chr7.3:13362671-13362690 | None:intergenic | 15.0% |
| ! | AAAAGTGAAGCTGTAAAAAA+TGG | + | chr7.3:13362434-13362453 | MS.gene018614:CDS | 25.0% |
| ! | AAGATTTAACATCATTCTCA+TGG | + | chr7.3:13362490-13362509 | MS.gene018614:CDS | 25.0% |
| ! | TTAAAATGAGATTCACTTGT+TGG | + | chr7.3:13362684-13362703 | MS.gene018614:intron | 25.0% |
| !! | AATTCAGTTCCTAAATTTTG+AGG | - | chr7.3:13362341-13362360 | None:intergenic | 25.0% |
| !! | ATAATGGAACCTCAAAATTT+AGG | + | chr7.3:13362329-13362348 | MS.gene018614:CDS | 25.0% |
| !!! | TTGTTGGATTTTTTTAATGC+AGG | + | chr7.3:13362700-13362719 | MS.gene018614:intron | 25.0% |
| AGCAGAAAAGCAAGAATTTA+TGG | + | chr7.3:13362787-13362806 | MS.gene018614:CDS | 30.0% | |
| TAAATCTTTGACCATTGCAA+TGG | - | chr7.3:13362478-13362497 | None:intergenic | 30.0% | |
| TAACATCATTCTCATGGATA+AGG | + | chr7.3:13362496-13362515 | MS.gene018614:CDS | 30.0% | |
| AAGCAAGAATTTATGGAAGC+AGG | + | chr7.3:13362794-13362813 | MS.gene018614:CDS | 35.0% | |
| AAGGAAATGCCTATCATGAA+TGG | + | chr7.3:13362515-13362534 | MS.gene018614:CDS | 35.0% | |
| AATCGAATTAAGCACACCAA+TGG | - | chr7.3:13362849-13362868 | None:intergenic | 35.0% | |
| AGCAAGAATTTATGGAAGCA+GGG | + | chr7.3:13362795-13362814 | MS.gene018614:CDS | 35.0% | |
| AGGAAATGCCTATCATGAAT+GGG | + | chr7.3:13362516-13362535 | MS.gene018614:CDS | 35.0% | |
| CTTGTTCGAATGATTCATCA+AGG | + | chr7.3:13362389-13362408 | MS.gene018614:CDS | 35.0% | |
| AAGATCAAGGGAAAGGGAAA+GGG | + | chr7.3:13362306-13362325 | MS.gene018614:CDS | 40.0% | |
| ACACACCAACAATCATGCTA+CGG | - | chr7.3:13362754-13362773 | None:intergenic | 40.0% | |
| ACCTCAATCCCATTCATGAT+AGG | - | chr7.3:13362527-13362546 | None:intergenic | 40.0% | |
| CAAGGTCTATTGAAAAGAGC+TGG | + | chr7.3:13362407-13362426 | MS.gene018614:CDS | 40.0% | |
| CAAGTGAAACCATTGACCAT+TGG | + | chr7.3:13362830-13362849 | MS.gene018614:CDS | 40.0% | |
| GCTGTAGATATCCATTGCAA+TGG | + | chr7.3:13362464-13362483 | MS.gene018614:CDS | 40.0% | |
| TTAAGCACACCAATGGTCAA+TGG | - | chr7.3:13362842-13362861 | None:intergenic | 40.0% | |
| AACCAAGAAACTGCGATCGA+TGG | + | chr7.3:13362724-13362743 | MS.gene018614:CDS | 45.0% | |
| ACCAAGAAACTGCGATCGAT+GGG | + | chr7.3:13362725-13362744 | MS.gene018614:CDS | 45.0% | |
| AGGGAAAGGGAAAGGGATAA+TGG | + | chr7.3:13362313-13362332 | MS.gene018614:CDS | 45.0% | |
| GAAGATCAAGGGAAAGGGAA+AGG | + | chr7.3:13362305-13362324 | MS.gene018614:CDS | 45.0% | |
| GCCTATCATGAATGGGATTG+AGG | + | chr7.3:13362523-13362542 | MS.gene018614:CDS | 45.0% | |
| GGTATCCGTAGCATGATTGT+TGG | + | chr7.3:13362746-13362765 | MS.gene018614:CDS | 45.0% | |
| ACCCATCGATCGCAGTTTCT+TGG | - | chr7.3:13362729-13362748 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 13362302 | 13362895 | 13362302 | ID=MS.gene018614 |
| chr7.3 | mRNA | 13362302 | 13362895 | 13362302 | ID=MS.gene018614.t1;Parent=MS.gene018614 |
| chr7.3 | exon | 13362302 | 13362544 | 13362302 | ID=MS.gene018614.t1.exon1;Parent=MS.gene018614.t1 |
| chr7.3 | CDS | 13362302 | 13362544 | 13362302 | ID=cds.MS.gene018614.t1;Parent=MS.gene018614.t1 |
| chr7.3 | exon | 13362722 | 13362895 | 13362722 | ID=MS.gene018614.t1.exon2;Parent=MS.gene018614.t1 |
| chr7.3 | CDS | 13362722 | 13362895 | 13362722 | ID=cds.MS.gene018614.t1;Parent=MS.gene018614.t1 |
| Gene Sequence |
| Protein sequence |
