Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018619.t1 | AES81629.1 | 93 | 86 | 6 | 0 | 38 | 123 | 11 | 96 | 8.00E-37 | 163.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018619.t1 | F4JZT3 | 39.2 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 4.8e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018619.t1 | G7KYG8 | 93.0 | 86 | 6 | 0 | 38 | 123 | 11 | 96 | 5.8e-37 | 163.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018619.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018619.t1 | MTR_7g098860 | 93.023 | 86 | 6 | 0 | 38 | 123 | 11 | 96 | 1.10e-53 | 164 |
| MS.gene018619.t1 | MTR_7g098910 | 47.500 | 120 | 58 | 3 | 16 | 135 | 22 | 136 | 2.75e-25 | 94.0 |
| MS.gene018619.t1 | MTR_7g099500 | 43.902 | 123 | 65 | 2 | 16 | 138 | 11 | 129 | 7.98e-25 | 92.4 |
| MS.gene018619.t1 | MTR_8g063580 | 45.545 | 101 | 52 | 2 | 16 | 114 | 12 | 111 | 8.40e-18 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018619.t1 | AT5G26594 | 39.167 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 2.70e-19 | 78.6 |
| MS.gene018619.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.40e-15 | 67.4 |
| MS.gene018619.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.40e-15 | 67.4 |
| MS.gene018619.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.40e-15 | 67.4 |
Find 32 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGGGACTCTGTGATGATA+TGG | 0.319136 | 7.3:-13463647 | None:intergenic |
| GCCCGTCATGGATGGAATTA+CGG | 0.406314 | 7.3:+13463841 | MS.gene018619:CDS |
| CTGCATATTCATCATTAGTA+AGG | 0.410681 | 7.3:-13464137 | None:intergenic |
| AGAGATATCTTGACATTACT+TGG | 0.437895 | 7.3:+13463722 | MS.gene018619:CDS |
| GAATACCCTGGCCAGATTGT+TGG | 0.444431 | 7.3:+13464102 | MS.gene018619:CDS |
| AACCGTGCCAATGAGGTCTA+AGG | 0.458884 | 7.3:-13463780 | None:intergenic |
| TATGCAGAATTTCGCAATGC+AGG | 0.461823 | 7.3:+13464153 | MS.gene018619:CDS |
| GACGTGAGACACCAACAATC+TGG | 0.490889 | 7.3:-13464113 | None:intergenic |
| GATGATCTACATCAAGAAGA+TGG | 0.493143 | 7.3:+13464180 | MS.gene018619:CDS |
| CCCGCGTGGATTAAAAGTAC+TGG | 0.511605 | 7.3:+13463664 | MS.gene018619:CDS |
| GAGACACCAACAATCTGGCC+AGG | 0.513873 | 7.3:-13464108 | None:intergenic |
| AAAGAAGCCTTAGACCTCAT+TGG | 0.516268 | 7.3:+13463773 | MS.gene018619:CDS |
| AGGATCATGCCCGTCATGGA+TGG | 0.527719 | 7.3:+13463833 | MS.gene018619:CDS |
| TATCTTGACATTACTTGGTG+TGG | 0.530817 | 7.3:+13463727 | MS.gene018619:CDS |
| TTTCAACATATGATAGTGCA+AGG | 0.532595 | 7.3:-13464208 | None:intergenic |
| AAAAGGTCATGCTAGTTGAC+AGG | 0.534345 | 7.3:-13464252 | None:intergenic |
| GGCTAGGATCATGCCCGTCA+TGG | 0.548604 | 7.3:+13463829 | MS.gene018619:CDS |
| ACCGTAATTCCATCCATGAC+GGG | 0.549235 | 7.3:-13463842 | None:intergenic |
| TGGATTAAAAGTACTGGTTG+TGG | 0.556379 | 7.3:+13463670 | MS.gene018619:CDS |
| ATGCAGAATTTCGCAATGCA+GGG | 0.581025 | 7.3:+13464154 | MS.gene018619:CDS |
| TGATCCTAGCCATGAGAATG+AGG | 0.586084 | 7.3:-13463817 | None:intergenic |
| TAAATTCGACCTCATTCTCA+TGG | 0.588335 | 7.3:+13463808 | MS.gene018619:CDS |
| TCGACCTCATTCTCATGGCT+AGG | 0.592585 | 7.3:+13463813 | MS.gene018619:CDS |
| AACCGTAATTCCATCCATGA+CGG | 0.593130 | 7.3:-13463843 | None:intergenic |
| TACGTTCAACCGTGCCAATG+AGG | 0.601575 | 7.3:-13463787 | None:intergenic |
| AGCCTTAGACCTCATTGGCA+CGG | 0.608494 | 7.3:+13463778 | MS.gene018619:CDS |
| AGATGATGATCTTGTTCGAA+TGG | 0.623757 | 7.3:+13463694 | MS.gene018619:CDS |
| TTCAACATATGATAGTGCAA+GGG | 0.630612 | 7.3:-13464207 | None:intergenic |
| AGACACCAACAATCTGGCCA+GGG | 0.657108 | 7.3:-13464107 | None:intergenic |
| GGGACTCTGTGATGATATGG+AGG | 0.663830 | 7.3:-13463644 | None:intergenic |
| CTGCGCGATTTAGAATACCC+TGG | 0.695519 | 7.3:+13464090 | MS.gene018619:CDS |
| TATCATCACAGAGTCCCGCG+TGG | 0.741838 | 7.3:+13463650 | MS.gene018619:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATGAATGATTATATGCT+AGG | - | chr7.3:13464019-13464038 | None:intergenic | 20.0% |
| !! | TAATTACATACATACATACA+CGG | + | chr7.3:13463877-13463896 | MS.gene018619:intron | 20.0% |
| !!! | AATTGCTTTAAACTAATTCA+TGG | - | chr7.3:13463915-13463934 | None:intergenic | 20.0% |
| !!! | ATTGCTTTAAACTAATTCAT+GGG | - | chr7.3:13463914-13463933 | None:intergenic | 20.0% |
| !!! | GTTGAAACTAATTTTGAAAA+GGG | + | chr7.3:13464224-13464243 | MS.gene018619:CDS | 20.0% |
| !!! | TGTTGAAACTAATTTTGAAA+AGG | + | chr7.3:13464223-13464242 | MS.gene018619:CDS | 20.0% |
| AGAGATATCTTGACATTACT+TGG | + | chr7.3:13463722-13463741 | MS.gene018619:CDS | 30.0% | |
| ATGAACAGAAAGAAAATGCA+TGG | + | chr7.3:13463977-13463996 | MS.gene018619:intron | 30.0% | |
| CTGCATATTCATCATTAGTA+AGG | - | chr7.3:13464140-13464159 | None:intergenic | 30.0% | |
| TGAACAGAAAGAAAATGCAT+GGG | + | chr7.3:13463978-13463997 | MS.gene018619:intron | 30.0% | |
| ! | TTCAACATATGATAGTGCAA+GGG | - | chr7.3:13464210-13464229 | None:intergenic | 30.0% |
| ! | TTTCAACATATGATAGTGCA+AGG | - | chr7.3:13464211-13464230 | None:intergenic | 30.0% |
| AGATGATGATCTTGTTCGAA+TGG | + | chr7.3:13463694-13463713 | MS.gene018619:CDS | 35.0% | |
| GAACAGAAAGAAAATGCATG+GGG | + | chr7.3:13463979-13463998 | MS.gene018619:intron | 35.0% | |
| GATGATCTACATCAAGAAGA+TGG | + | chr7.3:13464180-13464199 | MS.gene018619:CDS | 35.0% | |
| TAAATTCGACCTCATTCTCA+TGG | + | chr7.3:13463808-13463827 | MS.gene018619:CDS | 35.0% | |
| ! | TATCTTGACATTACTTGGTG+TGG | + | chr7.3:13463727-13463746 | MS.gene018619:CDS | 35.0% |
| !! | TGGATTAAAAGTACTGGTTG+TGG | + | chr7.3:13463670-13463689 | MS.gene018619:CDS | 35.0% |
| AAAGAAGCCTTAGACCTCAT+TGG | + | chr7.3:13463773-13463792 | MS.gene018619:CDS | 40.0% | |
| AACCGTAATTCCATCCATGA+CGG | - | chr7.3:13463846-13463865 | None:intergenic | 40.0% | |
| ATGCAGAATTTCGCAATGCA+GGG | + | chr7.3:13464154-13464173 | MS.gene018619:CDS | 40.0% | |
| GAAACTCACGCAGTCAAAAA+TGG | + | chr7.3:13463749-13463768 | MS.gene018619:CDS | 40.0% | |
| TATGCAGAATTTCGCAATGC+AGG | + | chr7.3:13464153-13464172 | MS.gene018619:CDS | 40.0% | |
| ! | ACCAGTACTTTTAATCCACG+CGG | - | chr7.3:13463668-13463687 | None:intergenic | 40.0% |
| ACCGTAATTCCATCCATGAC+GGG | - | chr7.3:13463845-13463864 | None:intergenic | 45.0% | |
| CATTCATATGTGTGCACTGC+AGG | + | chr7.3:13464056-13464075 | MS.gene018619:intron | 45.0% | |
| GTCATGGATGGAATTACGGT+TGG | + | chr7.3:13463845-13463864 | MS.gene018619:intron | 45.0% | |
| TGATCCTAGCCATGAGAATG+AGG | - | chr7.3:13463820-13463839 | None:intergenic | 45.0% | |
| ! | CCAGTACTTTTAATCCACGC+GGG | - | chr7.3:13463667-13463686 | None:intergenic | 45.0% |
| AACCGTGCCAATGAGGTCTA+AGG | - | chr7.3:13463783-13463802 | None:intergenic | 50.0% | |
| AGACACCAACAATCTGGCCA+GGG | - | chr7.3:13464110-13464129 | None:intergenic | 50.0% | |
| AGCCTTAGACCTCATTGGCA+CGG | + | chr7.3:13463778-13463797 | MS.gene018619:CDS | 50.0% | |
| GAATACCCTGGCCAGATTGT+TGG | + | chr7.3:13464102-13464121 | MS.gene018619:CDS | 50.0% | |
| GACGTGAGACACCAACAATC+TGG | - | chr7.3:13464116-13464135 | None:intergenic | 50.0% | |
| GCCCGTCATGGATGGAATTA+CGG | + | chr7.3:13463841-13463860 | MS.gene018619:CDS | 50.0% | |
| GGGACTCTGTGATGATATGG+AGG | - | chr7.3:13463647-13463666 | None:intergenic | 50.0% | |
| TACGTTCAACCGTGCCAATG+AGG | - | chr7.3:13463790-13463809 | None:intergenic | 50.0% | |
| TCGACCTCATTCTCATGGCT+AGG | + | chr7.3:13463813-13463832 | MS.gene018619:CDS | 50.0% | |
| ! | CCCGCGTGGATTAAAAGTAC+TGG | + | chr7.3:13463664-13463683 | MS.gene018619:CDS | 50.0% |
| ! | CTGCGCGATTTAGAATACCC+TGG | + | chr7.3:13464090-13464109 | MS.gene018619:CDS | 50.0% |
| AGGATCATGCCCGTCATGGA+TGG | + | chr7.3:13463833-13463852 | MS.gene018619:CDS | 55.0% | |
| CGCGGGACTCTGTGATGATA+TGG | - | chr7.3:13463650-13463669 | None:intergenic | 55.0% | |
| GAGACACCAACAATCTGGCC+AGG | - | chr7.3:13464111-13464130 | None:intergenic | 55.0% | |
| TATCATCACAGAGTCCCGCG+TGG | + | chr7.3:13463650-13463669 | MS.gene018619:CDS | 55.0% | |
| GGCTAGGATCATGCCCGTCA+TGG | + | chr7.3:13463829-13463848 | MS.gene018619:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 13463629 | 13464263 | 13463629 | ID=MS.gene018619 |
| chr7.3 | mRNA | 13463629 | 13464263 | 13463629 | ID=MS.gene018619.t1;Parent=MS.gene018619 |
| chr7.3 | exon | 13463629 | 13463862 | 13463629 | ID=MS.gene018619.t1.exon1;Parent=MS.gene018619.t1 |
| chr7.3 | CDS | 13463629 | 13463862 | 13463629 | ID=cds.MS.gene018619.t1;Parent=MS.gene018619.t1 |
| chr7.3 | exon | 13464078 | 13464263 | 13464078 | ID=MS.gene018619.t1.exon2;Parent=MS.gene018619.t1 |
| chr7.3 | CDS | 13464078 | 13464263 | 13464078 | ID=cds.MS.gene018619.t1;Parent=MS.gene018619.t1 |
| Gene Sequence |
| Protein sequence |
