AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene01894

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01894.t1 MTR_6g051425 72.961 233 59 1 1 229 1 233 6.09e-123 365
MS.gene01894.t1 MTR_2g039370 60.526 114 44 1 9 122 59 171 1.69e-35 135
MS.gene01894.t1 MTR_3g465760 72.059 68 15 1 42 105 3 70 6.40e-26 101
MS.gene01894.t1 MTR_1g013060 27.536 207 140 4 19 218 26 229 2.27e-19 87.8
MS.gene01894.t1 MTR_4g035820 26.882 279 182 10 18 283 76 345 1.95e-18 89.4
MS.gene01894.t1 MTR_1g070355 27.885 208 140 6 27 229 84 286 1.10e-17 86.7
MS.gene01894.t1 MTR_1g083640 25.652 230 152 4 22 232 56 285 1.23e-17 84.0
MS.gene01894.t1 MTR_0558s0020 26.244 221 144 4 28 229 47 267 2.56e-17 83.2
MS.gene01894.t1 MTR_7g446520 26.840 231 143 4 2 229 77 284 4.25e-15 77.0
MS.gene01894.t1 MTR_8g446020 25.806 186 126 2 56 229 42 227 2.53e-14 73.6
MS.gene01894.t1 MTR_0402s0040 29.412 170 106 6 29 191 38 200 6.24e-13 71.6
MS.gene01894.t1 MTR_7g446480 29.412 136 96 0 101 236 149 284 1.06e-12 69.7
MS.gene01894.t1 MTR_0337s0040 27.778 144 103 1 83 225 81 224 1.08e-12 68.2
MS.gene01894.t1 MTR_6g090505 24.107 224 127 4 64 244 21 244 1.56e-12 69.7
MS.gene01894.t1 MTR_7g446510 27.647 170 122 1 67 236 131 299 5.18e-12 67.8
MS.gene01894.t1 MTR_1g008290 26.316 114 83 1 101 214 32 144 5.48e-12 64.3
MS.gene01894.t1 MTR_5g076070 27.397 146 99 3 87 229 124 265 1.03e-11 66.2
MS.gene01894.t1 MTR_3g450850 24.583 240 166 7 17 244 47 283 1.36e-11 66.6
MS.gene01894.t1 MTR_2g098880 31.818 110 65 3 18 121 62 167 2.79e-11 64.3
MS.gene01894.t1 MTR_2g098880 31.818 110 65 3 18 121 74 179 3.45e-11 64.3
MS.gene01894.t1 MTR_2g014100 25.789 190 122 4 22 192 49 238 9.24e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene01894.t1 AT4G38180 38.596 171 100 3 62 228 117 286 4.43e-27 116
MS.gene01894.t1 AT4G38180 26.667 225 142 4 231 433 420 643 1.51e-19 93.2
MS.gene01894.t1 AT2G27110 27.857 280 174 7 234 488 225 501 3.11e-24 107
MS.gene01894.t1 AT2G27110 27.500 280 175 7 234 488 370 646 3.74e-24 107
MS.gene01894.t1 AT2G27110 29.496 139 86 2 90 228 107 233 5.00e-11 66.2
MS.gene01894.t1 AT2G27110 27.500 280 175 7 234 488 370 646 3.74e-24 107
MS.gene01894.t1 AT2G27110 29.496 139 86 2 90 228 107 233 5.00e-11 66.2
MS.gene01894.t1 AT4G38170 23.394 218 141 5 231 425 185 399 3.95e-18 88.2
MS.gene01894.t1 AT4G15090 27.982 218 134 5 232 428 397 612 5.47e-17 85.1
MS.gene01894.t1 AT4G15090 26.484 219 143 5 20 229 54 263 1.32e-15 80.9
MS.gene01894.t1 AT4G15090 27.982 218 134 5 232 428 397 612 5.47e-17 85.1
MS.gene01894.t1 AT4G15090 26.484 219 143 5 20 229 54 263 1.32e-15 80.9
MS.gene01894.t1 AT4G15090 27.982 218 134 5 232 428 397 612 5.47e-17 85.1
MS.gene01894.t1 AT4G15090 26.484 219 143 5 20 229 54 263 1.32e-15 80.9
MS.gene01894.t1 AT4G15090 27.982 218 134 5 232 428 397 612 5.47e-17 85.1
MS.gene01894.t1 AT4G15090 26.484 219 143 5 20 229 54 263 1.32e-15 80.9
MS.gene01894.t1 AT4G15090 27.982 218 134 5 232 428 397 612 5.47e-17 85.1
MS.gene01894.t1 AT4G15090 26.484 219 143 5 20 229 54 263 1.32e-15 80.9
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 287 503 7.11e-17 84.7
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 344 560 7.92e-17 84.7
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 344 560 7.92e-17 84.7
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 358 574 8.39e-17 84.3
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 344 560 8.64e-17 84.3
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 344 560 1.21e-16 84.0
MS.gene01894.t1 AT4G19990 27.854 219 134 5 230 426 344 560 1.21e-16 84.0
MS.gene01894.t1 AT1G76320 26.977 215 132 4 232 425 334 544 2.16e-16 83.2
MS.gene01894.t1 AT1G76320 27.317 205 136 5 27 229 7 200 4.14e-12 69.7
MS.gene01894.t1 AT1G76320 26.977 215 132 4 232 425 334 544 2.16e-16 83.2
MS.gene01894.t1 AT1G76320 27.317 205 136 5 27 229 7 200 4.14e-12 69.7
MS.gene01894.t1 AT1G76320 26.977 215 132 4 232 425 334 544 2.24e-16 83.2
MS.gene01894.t1 AT1G76320 27.317 205 136 5 27 229 7 200 6.32e-12 68.9
MS.gene01894.t1 AT1G76320 26.977 215 132 4 232 425 334 544 2.29e-16 83.2
MS.gene01894.t1 AT1G76320 27.317 205 136 5 27 229 7 200 4.10e-12 69.7
MS.gene01894.t1 AT3G06250 28.767 219 115 6 235 424 497 703 2.47e-16 83.2
MS.gene01894.t1 AT3G06250 26.540 211 116 4 18 228 191 362 5.87e-13 72.4
MS.gene01894.t1 AT3G06250 28.767 219 115 6 235 424 497 703 2.47e-16 83.2
MS.gene01894.t1 AT3G06250 26.540 211 116 4 18 228 191 362 5.87e-13 72.4
MS.gene01894.t1 AT3G06250 28.767 219 115 6 235 424 497 703 2.47e-16 83.2
MS.gene01894.t1 AT3G06250 26.540 211 116 4 18 228 191 362 5.87e-13 72.4
MS.gene01894.t1 AT5G18960 27.397 219 118 5 235 424 521 727 1.03e-15 81.3
MS.gene01894.t1 AT5G18960 27.397 219 118 5 235 424 521 727 1.03e-15 81.3
MS.gene01894.t1 AT3G22170 28.440 218 127 5 233 426 407 619 2.34e-15 80.1
MS.gene01894.t1 AT3G22170 28.440 218 127 5 233 426 407 619 2.34e-15 80.1
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 300 576 2.81e-14 76.6
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 300 576 2.91e-14 76.3
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 341 617 3.19e-14 76.3
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 341 617 3.19e-14 76.3
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 341 617 3.28e-14 76.3
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 341 617 3.52e-14 76.3
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 341 617 3.52e-14 76.3
MS.gene01894.t1 AT2G32250 23.793 290 184 7 232 497 341 617 3.52e-14 76.3
MS.gene01894.t1 AT1G10240 28.372 215 147 4 18 226 49 262 1.63e-13 73.9
MS.gene01894.t1 AT1G10240 25.818 275 163 10 231 484 398 652 2.89e-12 70.1
MS.gene01894.t1 AT1G10240 28.372 215 147 4 18 226 49 262 1.63e-13 73.9
MS.gene01894.t1 AT1G10240 25.818 275 163 10 231 484 398 652 2.89e-12 70.1
MS.gene01894.t1 AT5G28530 25.991 227 151 4 18 229 56 280 1.86e-13 73.9
MS.gene01894.t1 AT5G28530 25.991 227 151 4 18 229 56 280 1.86e-13 73.9
MS.gene01894.t1 AT5G28530 25.991 227 151 4 18 229 56 280 1.90e-13 73.6
MS.gene01894.t1 AT5G28530 28.729 181 120 4 18 192 56 233 1.19e-11 67.8
MS.gene01894.t1 AT5G28530 28.729 181 120 4 18 192 56 233 1.28e-11 67.8
MS.gene01894.t1 AT1G52520 27.358 212 140 6 19 229 87 285 9.41e-11 65.1

Find 94 sgRNAs with CRISPR-Local

Find 158 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAAATATTTGGAATTATTT+AGG 0.090273 2.2:-62518636 MS.gene01894:CDS
TTGCAGGAATCTTGTAATTT+TGG 0.216335 2.2:-62516501 MS.gene01894:intron
GTATACGAGTTACAAATATT+TGG 0.231098 2.2:-62518647 MS.gene01894:CDS
TTGCGGATTTACAATATTAA+AGG 0.235308 2.2:-62517437 MS.gene01894:CDS
CAAATATTTGGAATTATTTA+GGG 0.245589 2.2:-62518635 MS.gene01894:CDS
CTCGTTTGACTAATGTCTTT+TGG 0.253639 2.2:-62518434 MS.gene01894:CDS
TTGTCACAAGTTCGAATCTA+TGG 0.257274 2.2:-62517480 MS.gene01894:CDS
ATTATTCTTAAACCCTTCTT+TGG 0.257903 2.2:+62518890 None:intergenic
TTTGGACTCATCCACTTCTA+TGG 0.267599 2.2:-62517513 MS.gene01894:CDS
GTCTTTATACAATTTAGTTT+TGG 0.305862 2.2:-62517252 MS.gene01894:CDS
ATGTTAGCAAGGAAGGAGTT+TGG 0.356508 2.2:-62518797 MS.gene01894:CDS
GAAGGATAAACATTAGTATA+AGG 0.359805 2.2:+62516807 None:intergenic
AACATCTTGTTAAGATGTAT+TGG 0.361271 2.2:+62517396 None:intergenic
GGGTTTAAGAATAATGAATA+TGG 0.371754 2.2:-62518882 MS.gene01894:CDS
TTCATTTGATGCATCTCAAC+AGG 0.378264 2.2:-62516787 MS.gene01894:intron
GCAACAACATCCTTCAAGTT+GGG 0.389027 2.2:-62516682 MS.gene01894:CDS
ACGGGACCTCATAAAGTCTA+TGG 0.389034 2.2:-62518679 MS.gene01894:CDS
AACAGTAAAACTCTTTAAAA+CGG 0.389800 2.2:+62518913 None:intergenic
ATCACCATCAGTCATTGAAT+TGG 0.389903 2.2:+62517310 None:intergenic
GAAAAGGTGGCGACAATTAG+AGG 0.395021 2.2:-62517765 MS.gene01894:CDS
ATGAATACATATCAACTTCA+TGG 0.400065 2.2:-62517970 MS.gene01894:CDS
ATGACTGATGGTGATGATTC+CGG 0.408403 2.2:-62517302 MS.gene01894:CDS
TTTAGTGGCATTTGAGAAGA+TGG 0.409481 2.2:-62517789 MS.gene01894:intron
TTCTTTGCAGTCATGCTTTG+CGG 0.424200 2.2:-62517454 MS.gene01894:CDS
CAAGATTTAGTGTAAGAAAA+GGG 0.432600 2.2:-62518971 MS.gene01894:CDS
AAGTTGAAATTGATTGAAGC+TGG 0.434164 2.2:-62518543 MS.gene01894:CDS
TGTCAATCAGTTGAAATTGA+TGG 0.438803 2.2:-62519074 MS.gene01894:CDS
ACAAGATTTAGTGTAAGAAA+AGG 0.440176 2.2:-62518972 MS.gene01894:CDS
AATTCACCATAGACTTTATG+AGG 0.446268 2.2:+62518673 None:intergenic
ACAGTAAAACTCTTTAAAAC+GGG 0.452807 2.2:+62518914 None:intergenic
GACATTTGACATATTTCTCA+TGG 0.454279 2.2:+62518704 None:intergenic
CTACGTTGATAATTGCTACT+TGG 0.456826 2.2:+62516468 None:intergenic
CCTGGCTGAAATAATTGAAC+GGG 0.458030 2.2:+62516610 None:intergenic
GCATTTAAACCCCAACTTGA+AGG 0.462159 2.2:+62516672 None:intergenic
AAATATGTCAAATGTCAAAC+GGG 0.464386 2.2:-62518697 MS.gene01894:CDS
AGTTGAAATTGATTGAAGCT+GGG 0.466350 2.2:-62518542 MS.gene01894:CDS
TGTATGCAATTCGCAGCAAA+TGG 0.471481 2.2:-62517927 MS.gene01894:CDS
TCATTTAGTAACTTGCTACA+TGG 0.476165 2.2:-62516639 MS.gene01894:CDS
GTCGGTGGATATAACAATCT+TGG 0.477476 2.2:-62518606 MS.gene01894:CDS
CTTATGAAAGAGCTGATTCA+AGG 0.480001 2.2:-62518848 MS.gene01894:CDS
CGCAACAAATGTCTTTGCTC+AGG 0.482031 2.2:+62518729 None:intergenic
CTTGTTAAGATGTATTGGTC+AGG 0.483267 2.2:+62517401 None:intergenic
CCCGTTCAATTATTTCAGCC+AGG 0.484637 2.2:-62516610 MS.gene01894:intron
TTGGAATTATTTAGGGGAAG+AGG 0.485109 2.2:-62518628 MS.gene01894:CDS
GGAAGCACTTCCGTGGAGAT+TGG 0.486127 2.2:-62517605 MS.gene01894:CDS
GAAATATGTCAAATGTCAAA+CGG 0.486335 2.2:-62518698 MS.gene01894:CDS
TTACAGTGTGCAGCTAGATC+AGG 0.487131 2.2:-62518460 MS.gene01894:CDS
AATGAGTATCTTAATGGGGC+AGG 0.495180 2.2:-62517629 MS.gene01894:CDS
ATCAACTTCATGGTCACAAA+TGG 0.496700 2.2:-62517960 MS.gene01894:CDS
AGCAACAACATCCTTCAAGT+TGG 0.497300 2.2:-62516683 MS.gene01894:CDS
ACCATCAGTCATTGAATTGG+AGG 0.504486 2.2:+62517313 None:intergenic
CGTTGATAATTGCTACTTGG+TGG 0.516800 2.2:+62516471 None:intergenic
AGGTTTAATGTTAGCAAGGA+AGG 0.523844 2.2:-62518804 MS.gene01894:CDS
AAGAAGAATTTCAAACCACT+TGG 0.525111 2.2:-62518002 MS.gene01894:CDS
TGTTGAGATGCATCAAATGA+AGG 0.531567 2.2:+62516789 None:intergenic
CAACAACATCCTTCAAGTTG+GGG 0.535019 2.2:-62516681 MS.gene01894:CDS
AAGGACAAATTGCAAAGCTA+TGG 0.542290 2.2:-62518829 MS.gene01894:CDS
ACCTGGCTGAAATAATTGAA+CGG 0.555074 2.2:+62516609 None:intergenic
ATTATGATCTAATACAAACT+TGG 0.563904 2.2:+62518767 None:intergenic
AGCGTTGCGGTAAAGAAGAC+CGG 0.568368 2.2:+62517283 None:intergenic
ATTTAGTGTAAGAAAAGGGA+GGG 0.568911 2.2:-62518967 MS.gene01894:CDS
GGCGAGGTTTAATGTTAGCA+AGG 0.569412 2.2:-62518808 MS.gene01894:CDS
TGTCACAAGTTCGAATCTAT+GGG 0.570742 2.2:-62517479 MS.gene01894:CDS
TAATGAATATGGAGGTGAAG+TGG 0.572461 2.2:-62518871 MS.gene01894:CDS
GAGTATCTTAATGGGGCAGG+TGG 0.572748 2.2:-62517626 MS.gene01894:CDS
ACCTCCAATTCAATGACTGA+TGG 0.575513 2.2:-62517314 MS.gene01894:CDS
GATTTAGTGTAAGAAAAGGG+AGG 0.580368 2.2:-62518968 MS.gene01894:CDS
ACTGCAGCTAACCATAGAAG+TGG 0.582272 2.2:+62517502 None:intergenic
CTGGTATCAAATCTTCTCAA+AGG 0.583873 2.2:-62517873 MS.gene01894:CDS
TATTTAGGGGAAGAGGTCGG+TGG 0.586341 2.2:-62518621 MS.gene01894:CDS
GGCAGGTGGAAGCACTTCCG+TGG 0.590405 2.2:-62517612 MS.gene01894:CDS
ATAAAGTCTATGGTGAATTG+TGG 0.590947 2.2:-62518669 MS.gene01894:CDS
AATTATTTAGGGGAAGAGGT+CGG 0.592715 2.2:-62518624 MS.gene01894:CDS
GTTGAAATTGATTGAAGCTG+GGG 0.593382 2.2:-62518541 MS.gene01894:CDS
AGAAGAATTTCAAACCACTT+GGG 0.599091 2.2:-62518001 MS.gene01894:CDS
TAATTCTCAAGTGGGTGGAG+TGG 0.599522 2.2:-62516856 MS.gene01894:CDS
CATTTGAGAAGATGGTGAAA+AGG 0.603784 2.2:-62517781 MS.gene01894:CDS
TTTAAGAATAATGAATATGG+AGG 0.604513 2.2:-62518879 MS.gene01894:CDS
CTATGCAAGTAATTCTCAAG+TGG 0.611045 2.2:-62516865 MS.gene01894:intron
CTGAGCAAAGACATTTGTTG+CGG 0.611498 2.2:-62518728 MS.gene01894:CDS
GCAAGTAATTCTCAAGTGGG+TGG 0.619861 2.2:-62516861 MS.gene01894:intron
TTGAGAAGATGGTGAAAAGG+TGG 0.621585 2.2:-62517778 MS.gene01894:CDS
AATTCGCAGCAAATGGAGCA+CGG 0.624767 2.2:-62517920 MS.gene01894:CDS
TATGCAAGTAATTCTCAAGT+GGG 0.627225 2.2:-62516864 MS.gene01894:intron
CAAATTGCAAAGCTATGGCG+AGG 0.632194 2.2:-62518824 MS.gene01894:CDS
TTTAAATGAGTATCTTAATG+GGG 0.632939 2.2:-62517633 MS.gene01894:CDS
AAATATTTGGAATTATTTAG+GGG 0.632966 2.2:-62518634 MS.gene01894:CDS
TCACTACTGTTTATAACACG+AGG 0.639447 2.2:+62517707 None:intergenic
AACAAATGTCTTTGCTCAGG+AGG 0.641459 2.2:+62518732 None:intergenic
AACTCTACGACATGAATCTG+TGG 0.642020 2.2:-62517684 MS.gene01894:CDS
ATTCATCATCTCATCCCAAG+TGG 0.657667 2.2:+62517987 None:intergenic
ATCACATCGACCAATCTCCA+CGG 0.672242 2.2:+62517595 None:intergenic
CTACTGTTTATAACACGAGG+AGG 0.687262 2.2:+62517710 None:intergenic
AAGACTTCAGAATAGCGTTG+CGG 0.703840 2.2:+62517270 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACAAAAGAAAAAAAAATAA+AGG - chr2.2:62518562-62518581 MS.gene01894:CDS 10.0%
!! TAAATTATAATCAAAATACC+TGG + chr2.2:62518961-62518980 None:intergenic 15.0%
!!! AAACTTCAATTTCTTTTAAT+TGG - chr2.2:62517299-62517318 MS.gene01894:CDS 15.0%
!!! AAATATTTGGAATTATTTAG+GGG - chr2.2:62516916-62516935 MS.gene01894:intron 15.0%
!!! ACAAATATTTGGAATTATTT+AGG - chr2.2:62516914-62516933 MS.gene01894:intron 15.0%
!!! CAAATATTTGGAATTATTTA+GGG - chr2.2:62516915-62516934 MS.gene01894:intron 15.0%
!!! TTTTAAATGAGTATCTTAAT+GGG - chr2.2:62517916-62517935 MS.gene01894:CDS 15.0%
!!! TTTTTAAATGAGTATCTTAA+TGG - chr2.2:62517915-62517934 MS.gene01894:CDS 15.0%
!! ATTATGATCTAATACAAACT+TGG + chr2.2:62516786-62516805 None:intergenic 20.0%
!! TTATTTAATAAATGCATGAC+AGG - chr2.2:62517514-62517533 MS.gene01894:CDS 20.0%
!! TTTAAATGAGTATCTTAATG+GGG - chr2.2:62517917-62517936 MS.gene01894:CDS 20.0%
!! TTTAAGAATAATGAATATGG+AGG - chr2.2:62516671-62516690 MS.gene01894:CDS 20.0%
!!! AACAGTAAAACTCTTTAAAA+CGG + chr2.2:62516640-62516659 None:intergenic 20.0%
!!! CTGAAGTATTGTTATTTTAT+CGG - chr2.2:62519008-62519027 MS.gene01894:CDS 20.0%
!!! GTCTTTATACAATTTAGTTT+TGG - chr2.2:62518298-62518317 MS.gene01894:intron 20.0%
!!! TGATCTGTAAATATTGTTTT+TGG + chr2.2:62517364-62517383 None:intergenic 20.0%
! AAATATGTCAAATGTCAAAC+GGG - chr2.2:62516853-62516872 MS.gene01894:CDS 25.0%
! AACATCTTGTTAAGATGTAT+TGG + chr2.2:62518157-62518176 None:intergenic 25.0%
! ACAAATCATGTCATACTTAT+TGG + chr2.2:62517212-62517231 None:intergenic 25.0%
! ACAAGATTTAGTGTAAGAAA+AGG - chr2.2:62516578-62516597 MS.gene01894:intron 25.0%
! ATGAATACATATCAACTTCA+TGG - chr2.2:62517580-62517599 MS.gene01894:CDS 25.0%
! ATTATTCTTAAACCCTTCTT+TGG + chr2.2:62516663-62516682 None:intergenic 25.0%
! CAAGATTTAGTGTAAGAAAA+GGG - chr2.2:62516579-62516598 MS.gene01894:intron 25.0%
! GAAATATGTCAAATGTCAAA+CGG - chr2.2:62516852-62516871 MS.gene01894:CDS 25.0%
! GAAGGATAAACATTAGTATA+AGG + chr2.2:62518746-62518765 None:intergenic 25.0%
! GGGTTTAAGAATAATGAATA+TGG - chr2.2:62516668-62516687 MS.gene01894:CDS 25.0%
! GTATACGAGTTACAAATATT+TGG - chr2.2:62516903-62516922 MS.gene01894:intron 25.0%
! TTGCGGATTTACAATATTAA+AGG - chr2.2:62518113-62518132 MS.gene01894:intron 25.0%
!! AAAGTTGATTATGATTGCTT+TGG - chr2.2:62517154-62517173 MS.gene01894:intron 25.0%
!! AAGTTGATTATGATTGCTTT+GGG - chr2.2:62517155-62517174 MS.gene01894:intron 25.0%
!! TAAAAAGTATATCGTGACTT+TGG - chr2.2:62518019-62518038 MS.gene01894:intron 25.0%
!! TCATTGAATTTTATTGTTGC+AGG - chr2.2:62519033-62519052 MS.gene01894:CDS 25.0%
!!! AAAGCATTTTTAGAGAGTAT+GGG - chr2.2:62517328-62517347 MS.gene01894:CDS 25.0%
!!! ACAGTAAAACTCTTTAAAAC+GGG + chr2.2:62516639-62516658 None:intergenic 25.0%
!!! AGTCACGATATACTTTTTAT+TGG + chr2.2:62518018-62518037 None:intergenic 25.0%
!!! GTCACGATATACTTTTTATT+GGG + chr2.2:62518017-62518036 None:intergenic 25.0%
!!! TAAAGCATTTTTAGAGAGTA+TGG - chr2.2:62517327-62517346 MS.gene01894:CDS 25.0%
!!! TACACTAAATCTTGTTTTGA+AGG + chr2.2:62516573-62516592 None:intergenic 25.0%
!!! TCACGATATACTTTTTATTG+GGG + chr2.2:62518016-62518035 None:intergenic 25.0%
AAAAAATGCTCCTTCACATT+TGG - chr2.2:62517459-62517478 MS.gene01894:CDS 30.0%
AAAAATGCTCCTTCACATTT+GGG - chr2.2:62517460-62517479 MS.gene01894:CDS 30.0%
AAGAAGAATTTCAAACCACT+TGG - chr2.2:62517548-62517567 MS.gene01894:CDS 30.0%
AAGTTGAAATTGATTGAAGC+TGG - chr2.2:62517007-62517026 MS.gene01894:intron 30.0%
AATTCACCATAGACTTTATG+AGG + chr2.2:62516880-62516899 None:intergenic 30.0%
AGAAGAATTTCAAACCACTT+GGG - chr2.2:62517549-62517568 MS.gene01894:CDS 30.0%
AGTTGAAATTGATTGAAGCT+GGG - chr2.2:62517008-62517027 MS.gene01894:intron 30.0%
AGTTGATTATGATTGCTTTG+GGG - chr2.2:62517156-62517175 MS.gene01894:intron 30.0%
ATAAAGTCTATGGTGAATTG+TGG - chr2.2:62516881-62516900 MS.gene01894:intron 30.0%
ATGATTTATCCCAACAAATG+GGG + chr2.2:62517236-62517255 None:intergenic 30.0%
ATGTGAGAATTGCTTGATAT+TGG - chr2.2:62518353-62518372 MS.gene01894:intron 30.0%
ATTTAGTGTAAGAAAAGGGA+GGG - chr2.2:62516583-62516602 MS.gene01894:intron 30.0%
CAAGATGTTCAAAAAATGCA+AGG - chr2.2:62518168-62518187 MS.gene01894:intron 30.0%
GAACAATTTCTCACAAAATG+AGG - chr2.2:62518439-62518458 MS.gene01894:CDS 30.0%
GACATTTGACATATTTCTCA+TGG + chr2.2:62516849-62516868 None:intergenic 30.0%
GATGATTTATCCCAACAAAT+GGG + chr2.2:62517237-62517256 None:intergenic 30.0%
TATGCAAGTAATTCTCAAGT+GGG - chr2.2:62518686-62518705 MS.gene01894:CDS 30.0%
TCTTCAGTTGAATTTAAGTC+AGG + chr2.2:62518410-62518429 None:intergenic 30.0%
TGATGATTTATCCCAACAAA+TGG + chr2.2:62517238-62517257 None:intergenic 30.0%
TGTCAATCAGTTGAAATTGA+TGG - chr2.2:62516476-62516495 MS.gene01894:CDS 30.0%
TTGTTGGGATAAATCATCAT+TGG - chr2.2:62517239-62517258 MS.gene01894:CDS 30.0%
! AGTAATCATGTATTTTCTGC+TGG - chr2.2:62517658-62517677 MS.gene01894:CDS 30.0%
! AGTTTTACTGTTCCAAAGAA+GGG - chr2.2:62516648-62516667 MS.gene01894:CDS 30.0%
! GGATGCAAAGATATTTTTCT+TGG - chr2.2:62516497-62516516 MS.gene01894:CDS 30.0%
! TTGCAGGAATCTTGTAATTT+TGG - chr2.2:62519049-62519068 MS.gene01894:CDS 30.0%
! TTTTCTCAAAATGTCCTTTC+TGG + chr2.2:62518529-62518548 None:intergenic 30.0%
!! AGGAATCTTGTAATTTTGGT+CGG - chr2.2:62519053-62519072 MS.gene01894:CDS 30.0%
!! ATGTCTCTCACAATTTTTAG+TGG - chr2.2:62517746-62517765 MS.gene01894:CDS 30.0%
!! TCATTTAGTAACTTGCTACA+TGG - chr2.2:62518911-62518930 MS.gene01894:CDS 30.0%
AAGGACAAATTGCAAAGCTA+TGG - chr2.2:62516721-62516740 MS.gene01894:intron 35.0%
AATTATTTAGGGGAAGAGGT+CGG - chr2.2:62516926-62516945 MS.gene01894:intron 35.0%
ACCTGGCTGAAATAATTGAA+CGG + chr2.2:62518944-62518963 None:intergenic 35.0%
AGGTTTAATGTTAGCAAGGA+AGG - chr2.2:62516746-62516765 MS.gene01894:intron 35.0%
ATCAACTTCATGGTCACAAA+TGG - chr2.2:62517590-62517609 MS.gene01894:CDS 35.0%
ATCACCATCAGTCATTGAAT+TGG + chr2.2:62518243-62518262 None:intergenic 35.0%
CATTGGCAAAATGTCATGTT+TGG - chr2.2:62517256-62517275 MS.gene01894:CDS 35.0%
CATTTGAGAAGATGGTGAAA+AGG - chr2.2:62517769-62517788 MS.gene01894:CDS 35.0%
CTATGCAAGTAATTCTCAAG+TGG - chr2.2:62518685-62518704 MS.gene01894:CDS 35.0%
CTGGTATCAAATCTTCTCAA+AGG - chr2.2:62517677-62517696 MS.gene01894:CDS 35.0%
GATTTAGTGTAAGAAAAGGG+AGG - chr2.2:62516582-62516601 MS.gene01894:intron 35.0%
GCTTGATATTGGAGTTAAGA+GGG - chr2.2:62518364-62518383 MS.gene01894:intron 35.0%
GTTGAAATTGATTGAAGCTG+GGG - chr2.2:62517009-62517028 MS.gene01894:intron 35.0%
TAATGAATATGGAGGTGAAG+TGG - chr2.2:62516679-62516698 MS.gene01894:CDS 35.0%
TCACTACTGTTTATAACACG+AGG + chr2.2:62517846-62517865 None:intergenic 35.0%
TGCTTGATATTGGAGTTAAG+AGG - chr2.2:62518363-62518382 MS.gene01894:intron 35.0%
TGTCACAAGTTCGAATCTAT+GGG - chr2.2:62518071-62518090 MS.gene01894:intron 35.0%
TGTTGAGATGCATCAAATGA+AGG + chr2.2:62518764-62518783 None:intergenic 35.0%
TTCATTTGATGCATCTCAAC+AGG - chr2.2:62518763-62518782 MS.gene01894:CDS 35.0%
TTGGAATTATTTAGGGGAAG+AGG - chr2.2:62516922-62516941 MS.gene01894:intron 35.0%
TTGTCACAAGTTCGAATCTA+TGG - chr2.2:62518070-62518089 MS.gene01894:intron 35.0%
TTTAGTGGCATTTGAGAAGA+TGG - chr2.2:62517761-62517780 MS.gene01894:CDS 35.0%
! CTCGTTTGACTAATGTCTTT+TGG - chr2.2:62517116-62517135 MS.gene01894:intron 35.0%
! CTTATGAAAGAGCTGATTCA+AGG - chr2.2:62516702-62516721 MS.gene01894:intron 35.0%
! CTTGTTAAGATGTATTGGTC+AGG + chr2.2:62518152-62518171 None:intergenic 35.0%
! GAGTTTTACTGTTCCAAAGA+AGG - chr2.2:62516647-62516666 MS.gene01894:CDS 35.0%
! TGAGAGTACAATAGTGCTTT+GGG - chr2.2:62517702-62517721 MS.gene01894:CDS 35.0%
!! ACTAATGTCTTTTGGAGAGA+TGG - chr2.2:62517124-62517143 MS.gene01894:intron 35.0%
!! CTACGTTGATAATTGCTACT+TGG + chr2.2:62519085-62519104 None:intergenic 35.0%
!!! CAATTTAGTTTTGGAATCCC+AGG - chr2.2:62518307-62518326 MS.gene01894:intron 35.0%
AACAAATGTCTTTGCTCAGG+AGG + chr2.2:62516821-62516840 None:intergenic 40.0%
AACTCTACGACATGAATCTG+TGG - chr2.2:62517866-62517885 MS.gene01894:intron 40.0%
AAGACTTCAGAATAGCGTTG+CGG + chr2.2:62518283-62518302 None:intergenic 40.0%
AATGAGTATCTTAATGGGGC+AGG - chr2.2:62517921-62517940 MS.gene01894:CDS 40.0%
ACAGATCAAGATCCAGCTAT+GGG - chr2.2:62517376-62517395 MS.gene01894:CDS 40.0%
ACCATCAGTCATTGAATTGG+AGG + chr2.2:62518240-62518259 None:intergenic 40.0%
ACCTCCAATTCAATGACTGA+TGG - chr2.2:62518236-62518255 MS.gene01894:intron 40.0%
AGCAACAACATCCTTCAAGT+TGG - chr2.2:62518867-62518886 MS.gene01894:CDS 40.0%
ATGTTAGCAAGGAAGGAGTT+TGG - chr2.2:62516753-62516772 MS.gene01894:intron 40.0%
ATTCATCATCTCATCCCAAG+TGG + chr2.2:62517566-62517585 None:intergenic 40.0%
CCTGGCTGAAATAATTGAAC+GGG + chr2.2:62518943-62518962 None:intergenic 40.0%
CTACTGTTTATAACACGAGG+AGG + chr2.2:62517843-62517862 None:intergenic 40.0%
GCATTTAAACCCCAACTTGA+AGG + chr2.2:62518881-62518900 None:intergenic 40.0%
GTCGGTGGATATAACAATCT+TGG - chr2.2:62516944-62516963 MS.gene01894:intron 40.0%
TACAGATCAAGATCCAGCTA+TGG - chr2.2:62517375-62517394 MS.gene01894:CDS 40.0%
TGTATGCAATTCGCAGCAAA+TGG - chr2.2:62517623-62517642 MS.gene01894:CDS 40.0%
TTCTTTGCAGTCATGCTTTG+CGG - chr2.2:62518096-62518115 MS.gene01894:intron 40.0%
TTGAGAAGATGGTGAAAAGG+TGG - chr2.2:62517772-62517791 MS.gene01894:CDS 40.0%
TTGAGAGATCCCAAATGTGA+AGG + chr2.2:62517472-62517491 None:intergenic 40.0%
TTTGGACTCATCCACTTCTA+TGG - chr2.2:62518037-62518056 MS.gene01894:intron 40.0%
! ATGACTGATGGTGATGATTC+CGG - chr2.2:62518248-62518267 MS.gene01894:intron 40.0%
! ATGATTTGTGCCCCATTTGT+TGG - chr2.2:62517223-62517242 MS.gene01894:CDS 40.0%
! CTGAGCAAAGACATTTGTTG+CGG - chr2.2:62516822-62516841 MS.gene01894:CDS 40.0%
! GAAGGATGTTGTTGCTGTTA+AGG + chr2.2:62518863-62518882 None:intergenic 40.0%
! GCAACAACATCCTTCAAGTT+GGG - chr2.2:62518868-62518887 MS.gene01894:CDS 40.0%
! GTGAGAGTACAATAGTGCTT+TGG - chr2.2:62517701-62517720 MS.gene01894:CDS 40.0%
! GTTTGGTTGTGCTTTGTTGT+TGG - chr2.2:62517273-62517292 MS.gene01894:CDS 40.0%
! TGATTTGTGCCCCATTTGTT+GGG - chr2.2:62517224-62517243 MS.gene01894:CDS 40.0%
!! CAACAACATCCTTCAAGTTG+GGG - chr2.2:62518869-62518888 MS.gene01894:CDS 40.0%
!! CGTTGATAATTGCTACTTGG+TGG + chr2.2:62519082-62519101 None:intergenic 40.0%
!!! TTGTGCTTTTTTGTCCTCCT+GGG + chr2.2:62518327-62518346 None:intergenic 40.0%
!!! TTTAGTTTTGGAATCCCAGG+AGG - chr2.2:62518310-62518329 MS.gene01894:intron 40.0%
!!! TTTGTGCTTTTTTGTCCTCC+TGG + chr2.2:62518328-62518347 None:intergenic 40.0%
AATTCGCAGCAAATGGAGCA+CGG - chr2.2:62517630-62517649 MS.gene01894:CDS 45.0%
ACGGGACCTCATAAAGTCTA+TGG - chr2.2:62516871-62516890 MS.gene01894:intron 45.0%
ACTGCAGCTAACCATAGAAG+TGG + chr2.2:62518051-62518070 None:intergenic 45.0%
ATCACATCGACCAATCTCCA+CGG + chr2.2:62517958-62517977 None:intergenic 45.0%
CAAATTGCAAAGCTATGGCG+AGG - chr2.2:62516726-62516745 MS.gene01894:intron 45.0%
CACAGTCGATGACGTGTATT+TGG + chr2.2:62517424-62517443 None:intergenic 45.0%
CCCGTTCAATTATTTCAGCC+AGG - chr2.2:62518940-62518959 MS.gene01894:CDS 45.0%
CGCAACAAATGTCTTTGCTC+AGG + chr2.2:62516824-62516843 None:intergenic 45.0%
GAAAAGGTGGCGACAATTAG+AGG - chr2.2:62517785-62517804 MS.gene01894:CDS 45.0%
GCAAGTAATTCTCAAGTGGG+TGG - chr2.2:62518689-62518708 MS.gene01894:CDS 45.0%
GGCGAGGTTTAATGTTAGCA+AGG - chr2.2:62516742-62516761 MS.gene01894:intron 45.0%
GTTTCGCAAAGACGAAAGGA+AGG - chr2.2:62518492-62518511 MS.gene01894:CDS 45.0%
TAATTCTCAAGTGGGTGGAG+TGG - chr2.2:62518694-62518713 MS.gene01894:CDS 45.0%
TTACAGTGTGCAGCTAGATC+AGG - chr2.2:62517090-62517109 MS.gene01894:intron 45.0%
!! AGTGCTTTGGGAGATATTGC+AGG - chr2.2:62517714-62517733 MS.gene01894:CDS 45.0%
!! AAATATTAAATTTATAATGA+TGG + chr2.2:62518803-62518822 None:intergenic 5.0%
AGCGTTGCGGTAAAGAAGAC+CGG + chr2.2:62518270-62518289 None:intergenic 50.0%
AGGTCTAGTGTTCGCCAGAA+AGG - chr2.2:62518512-62518531 MS.gene01894:CDS 50.0%
CCAATTGCTTTGCCCATAGC+TGG + chr2.2:62517391-62517410 None:intergenic 50.0%
CCAGCTATGGGCAAAGCAAT+TGG - chr2.2:62517388-62517407 MS.gene01894:CDS 50.0%
CCTCGTTTCGCAAAGACGAA+AGG - chr2.2:62518488-62518507 MS.gene01894:CDS 50.0%
CCTTTCGTCTTTGCGAAACG+AGG + chr2.2:62518491-62518510 None:intergenic 50.0%
GAGTATCTTAATGGGGCAGG+TGG - chr2.2:62517924-62517943 MS.gene01894:CDS 50.0%
TATTTAGGGGAAGAGGTCGG+TGG - chr2.2:62516929-62516948 MS.gene01894:intron 50.0%
! CACGTCATCGACTGTGTTTG+TGG - chr2.2:62517428-62517447 MS.gene01894:CDS 50.0%
!! GGAAGCACTTCCGTGGAGAT+TGG - chr2.2:62517945-62517964 MS.gene01894:CDS 55.0%
!! GGCAGGTGGAAGCACTTCCG+TGG - chr2.2:62517938-62517957 MS.gene01894:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 62516461 62519111 62516461 ID=MS.gene01894
chr2.2 mRNA 62516461 62519111 62516461 ID=MS.gene01894.t1;Parent=MS.gene01894
chr2.2 exon 62518427 62519111 62518427 ID=MS.gene01894.t1.exon1;Parent=MS.gene01894.t1
chr2.2 CDS 62518427 62519111 62518427 ID=cds.MS.gene01894.t1;Parent=MS.gene01894.t1
chr2.2 exon 62517872 62518036 62517872 ID=MS.gene01894.t1.exon2;Parent=MS.gene01894.t1
chr2.2 CDS 62517872 62518036 62517872 ID=cds.MS.gene01894.t1;Parent=MS.gene01894.t1
chr2.2 exon 62517218 62517806 62517218 ID=MS.gene01894.t1.exon3;Parent=MS.gene01894.t1
chr2.2 CDS 62517218 62517806 62517218 ID=cds.MS.gene01894.t1;Parent=MS.gene01894.t1
chr2.2 exon 62516788 62516878 62516788 ID=MS.gene01894.t1.exon4;Parent=MS.gene01894.t1
chr2.2 CDS 62516788 62516878 62516788 ID=cds.MS.gene01894.t1;Parent=MS.gene01894.t1
chr2.2 exon 62516611 62516703 62516611 ID=MS.gene01894.t1.exon5;Parent=MS.gene01894.t1
chr2.2 CDS 62516611 62516703 62516611 ID=cds.MS.gene01894.t1;Parent=MS.gene01894.t1
chr2.2 exon 62516461 62516517 62516461 ID=MS.gene01894.t1.exon6;Parent=MS.gene01894.t1
chr2.2 CDS 62516461 62516517 62516461 ID=cds.MS.gene01894.t1;Parent=MS.gene01894.t1
Gene Sequence

>MS.gene01894

ATGGAGATAAATGAATGTCAATCAGTTGAAATTGATGGATGCAAAGATATTTTTCTTGGTCAAGTTGTGAATAACAAAGATGAAGCTTATAATTTATATCAAGCACATGCCTTCAAAACAAGATTTAGTGTAAGAAAAGGGAGGGAGTTATATTATGATAATGAGAGAAAAATCACCCGTTTTAAAGAGTTTTACTGTTCCAAAGAAGGGTTTAAGAATAATGAATATGGAGGTGAAGTGGCTTATGAAAGAGCTGATTCAAGGACAAATTGCAAAGCTATGGCGAGGTTTAATGTTAGCAAGGAAGGAGTTTGGAAAGTTACCAAGTTTGTATTAGATCATAATCATCAACTTGTACCTCCTGAGCAAAGACATTTGTTGCGGTCCATGAGAAATATGTCAAATGTCAAACGGGACCTCATAAAGTCTATGGTGAATTGTGGTATACGAGTTACAAATATTTGGAATTATTTAGGGGAAGAGGTCGGTGGATATAACAATCTTGGTATAACATTGAAAGATATGCATAATTATGTCTACACTGAAAAGTTGAAATTGATTGAAGCTGGGGATGCTCAATCTTTAGTAAATAACTTACAAAATAGACAAGCAAAAGATTCTATGTTCTATTACAGTGTGCAGCTAGATCAGGAATCTCGTTTGACTAATGTCTTTTGGAGAGATGGTAAATCTAAAGTTGATTATGATTGCTTTGGGGATGTTGTGATATTTGACACTACTTATCGTACCAATAAGTATGACATGATTTGTGCCCCATTTGTTGGGATAAATCATCATTGGCAAAATGTCATGTTTGGTTGTGCTTTGTTGTTGGATGAAACTTCAATTTCTTTTAATTGGTTGTTTAAAGCATTTTTAGAGAGTATGGGAAACAAACAACCAAAAACAATATTTACAGATCAAGATCCAGCTATGGGCAAAGCAATTGGAGAAGTAATGCCAAATACACGTCATCGACTGTGTTTGTGGCATATATCAAAAAATGCTCCTTCACATTTGGGATCTCTCAATTCTAATAACAAGTTTCAAAGCTTATTTAATAAATGCATGACAGGTTGTGACTCAGAAGAAGAATTTCAAACCACTTGGGATGAGATGATGAATACATATCAACTTCATGGTCACAAATGGTTAAGAAGCATGTATGCAATTCGCAGCAAATGGAGCACGGCATATAGTAATCATGTATTTTCTGCTGGTATCAAATCTTCTCAAAGGAGTGAGAGTACAATAGTGCTTTGGGAGATATTGCAGGAAAAACAACATGTCTCTCACAATTTTTAGTGGCATTTGAGAAGATGGTGAAAAGGTGGCGACAATTAGAGGTTGAGAACGAATTCAAAAATAGTCAGAGTGCGCCTCCTCGTGTTATAAACAGTAGTGAAACTCTACGACATGAATCTGTGGTATATACTCACAAAATCTTTACACTTTTTTTAAATGAGTATCTTAATGGGGCAGGTGGAAGCACTTCCGTGGAGATTGGTCGATGTGATAATGTGTCATATCATGAAGTTGTGTTGAACATCATCCCCAATAAAAAGTATATCGTGACTTTGGACTCATCCACTTCTATGGTTAGCTGCAGTTGTCACAAGTTCGAATCTATGGGCATTCTTTGCAGTCATGCTTTGCGGATTTACAATATTAAAGGTATTTTAAAAATACCTGACCAATACATCTTAACAAGATGTTCAAAAAATGCAAGGTCTGTGATTTATGAGCATGTTAATAAGAGTTCAGAAGAAGATTCTACCTCCAATTCAATGACTGATGGTGATGATTCCGGTCTTCTTTACCGCAACGCTATTCTGAAGTCTTTATACAATTTAGTTTTGGAATCCCAGGAGGACAAAAAAGCACAAAAAATCATGTGAGAATTGCTTGATATTGGAGTTAAGAGGGTTCGAGAATGTGTCAGCAAACCTGACTTAAATTCAACTGAAGAAAACATTGAGAACAATTTCTCACAAAATGAGGATAATGAAAGCTGTAAAGTTTTGAATCCTCGTTTCGCAAAGACGAAAGGAAGGTCTAGTGTTCGCCAGAAAGGACATTTTGAGAAAAGAAAATGATCAACTACAAAAGAAAAAAAAATAAAGGTGAAAAATTAAACATATTTTTTATAGAGTACATTATAGTTATATATTTAATGTAAGAACTATTTAATATCTGCTTTATTTTCAGAGAAACGTAATGAAGACTATGCAAGTAATTCTCAAGTGGGTGGAGTGGCAAATGTTCAAAATTGCACAAATGAACCTTATACTAATGTTTATCCTTCATTTGATGCATCTCAACAGGTTTATCACATTCTACCATCATTATAAATTTAATATTTCTTACAATTGTATTTTAGCTGACAATATTTTTGATATCCTTAACAGCAACAACATCCTTCAAGTTGGGGTTTAAATGCTACATCAACTTCATTTAGTAACTTGCTACATGGAATGAACCCGTTCAATTATTTCAGCCAGGTATTTTGATTATAATTTATTCTTGTTTTTTAAATGATATATTTGACTGAAGTATTGTTATTTTATCGGTATCATTGAATTTTATTGTTGCAGGAATCTTGTAATTTTGGTCGGATACCACCAAGTAGCAATTATCAACGTAGACAATGA
Protein sequence

>MS.gene01894.t1

MEINECQSVEIDGCKDIFLGQVVNNKDEAYNLYQAHAFKTRFSVRKGRELYYDNERKITRFKEFYCSKEGFKNNEYGGEVAYERADSRTNCKAMARFNVSKEGVWKVTKFVLDHNHQLVPPEQRHLLRSMRNMSNVKRDLIKSMVNCGIRVTNIWNYLGEEVGGYNNLGITLKDMHNYVYTEKLKLIEAGDAQSLVNNLQNRQAKDSMFYYSVQLDQESRLTNVFWRDGCDSEEEFQTTWDEMMNTYQLHGHKWLRSMYAIRSKWSTAYSNHVFSAGIKSSQRMAFEKMVKRWRQLEVENEFKNSQSAPPRVINSSETLRHESVVYTHKIFTLFLNEYLNGAGGSTSVEIGRCDNVSYHEVVLNIIPNKKYIVTLDSSTSMVSCSCHKFESMGILCSHALRIYNIKGILKIPDQYILTRCSKNARSVIYEHVNKSSEEDSTSNSMTDGDDSGLLYRNAILKSLYNLVLESQEDKKAQKIINSQVGGVANVQNCTNEPYTNVYPSFDASQQQQHPSSWGLNATSTSFSNLLHGMNPFNYFSQESCNFGRIPPSSNYQRRQ