Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018941.t1 | XP_027339360.1 | 90 | 90 | 9 | 0 | 1 | 90 | 1 | 90 | 6.60E-40 | 172.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018941.t1 | Q2Q493 | 77.3 | 88 | 16 | 2 | 1 | 87 | 1 | 85 | 2.8e-31 | 135.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018941.t1 | A0A151RXL6 | 90.0 | 90 | 9 | 0 | 1 | 90 | 1 | 90 | 1.1e-39 | 171.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018941.t1 | TF | zf-HD |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018941.t1 | MTR_3g039810 | 92.222 | 90 | 2 | 1 | 1 | 90 | 1 | 85 | 9.38e-56 | 167 |
| MS.gene018941.t1 | MTR_6g012220 | 67.123 | 73 | 16 | 2 | 1 | 73 | 1 | 65 | 9.84e-29 | 99.0 |
| MS.gene018941.t1 | MTR_3g100650 | 60.294 | 68 | 27 | 0 | 17 | 84 | 72 | 139 | 4.25e-24 | 92.0 |
| MS.gene018941.t1 | MTR_5g065440 | 65.517 | 58 | 20 | 0 | 23 | 80 | 56 | 113 | 7.80e-22 | 85.9 |
| MS.gene018941.t1 | MTR_1g077020 | 59.649 | 57 | 23 | 0 | 23 | 79 | 7 | 63 | 9.27e-21 | 81.6 |
| MS.gene018941.t1 | MTR_4g134580 | 55.405 | 74 | 33 | 0 | 6 | 79 | 94 | 167 | 2.80e-20 | 83.2 |
| MS.gene018941.t1 | MTR_2g076780 | 60.317 | 63 | 23 | 1 | 12 | 74 | 26 | 86 | 3.10e-20 | 81.3 |
| MS.gene018941.t1 | MTR_5g032600 | 61.404 | 57 | 22 | 0 | 24 | 80 | 77 | 133 | 2.43e-19 | 79.7 |
| MS.gene018941.t1 | MTR_7g010300 | 57.143 | 70 | 23 | 2 | 23 | 85 | 86 | 155 | 9.50e-19 | 78.6 |
| MS.gene018941.t1 | MTR_5g020510 | 57.377 | 61 | 26 | 0 | 18 | 78 | 92 | 152 | 1.64e-18 | 77.8 |
| MS.gene018941.t1 | MTR_8g069080 | 62.745 | 51 | 19 | 0 | 24 | 74 | 68 | 118 | 3.10e-18 | 76.6 |
| MS.gene018941.t1 | MTR_8g467340 | 56.667 | 60 | 24 | 1 | 23 | 80 | 62 | 121 | 7.07e-17 | 73.9 |
| MS.gene018941.t1 | MTR_6g012280 | 53.968 | 63 | 25 | 2 | 25 | 83 | 91 | 153 | 8.58e-16 | 70.5 |
| MS.gene018941.t1 | MTR_6g073013 | 52.381 | 63 | 29 | 1 | 12 | 73 | 6 | 68 | 1.89e-14 | 65.1 |
| MS.gene018941.t1 | MTR_7g070643 | 49.180 | 61 | 31 | 0 | 3 | 63 | 60 | 120 | 3.00e-12 | 59.3 |
| MS.gene018941.t1 | MTR_6g080300 | 45.902 | 61 | 33 | 0 | 3 | 63 | 32 | 92 | 4.09e-11 | 54.3 |
| MS.gene018941.t1 | MTR_8g442330 | 52.632 | 38 | 18 | 0 | 34 | 71 | 14 | 51 | 7.59e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018941.t1 | AT1G18835 | 77.273 | 88 | 16 | 2 | 1 | 87 | 1 | 85 | 3.68e-41 | 130 |
| MS.gene018941.t1 | AT1G74660 | 64.000 | 100 | 23 | 1 | 1 | 87 | 2 | 101 | 2.10e-39 | 126 |
| MS.gene018941.t1 | AT3G28917 | 63.636 | 99 | 26 | 3 | 1 | 89 | 1 | 99 | 5.42e-37 | 120 |
| MS.gene018941.t1 | AT1G74660 | 61.702 | 94 | 23 | 1 | 1 | 81 | 2 | 95 | 1.69e-33 | 111 |
| MS.gene018941.t1 | AT3G50890 | 58.824 | 68 | 28 | 0 | 8 | 75 | 43 | 110 | 1.34e-23 | 90.5 |
| MS.gene018941.t1 | AT4G24660 | 72.727 | 55 | 15 | 0 | 23 | 77 | 47 | 101 | 1.73e-23 | 89.4 |
| MS.gene018941.t1 | AT4G24660 | 72.727 | 55 | 15 | 0 | 23 | 77 | 47 | 101 | 2.56e-23 | 89.4 |
| MS.gene018941.t1 | AT2G02540 | 61.905 | 63 | 24 | 0 | 23 | 85 | 85 | 147 | 1.03e-22 | 89.0 |
| MS.gene018941.t1 | AT2G02540 | 61.905 | 63 | 24 | 0 | 23 | 85 | 85 | 147 | 1.03e-22 | 89.0 |
| MS.gene018941.t1 | AT2G18350 | 52.000 | 75 | 36 | 0 | 10 | 84 | 67 | 141 | 1.73e-21 | 85.1 |
| MS.gene018941.t1 | AT1G14440 | 62.712 | 59 | 22 | 0 | 23 | 81 | 88 | 146 | 2.60e-21 | 85.5 |
| MS.gene018941.t1 | AT1G14440 | 62.712 | 59 | 22 | 0 | 23 | 81 | 88 | 146 | 2.60e-21 | 85.5 |
| MS.gene018941.t1 | AT5G65410 | 67.925 | 53 | 17 | 0 | 23 | 75 | 73 | 125 | 4.68e-20 | 81.6 |
| MS.gene018941.t1 | AT1G75240 | 67.925 | 53 | 17 | 0 | 23 | 75 | 74 | 126 | 1.52e-19 | 80.5 |
| MS.gene018941.t1 | AT1G69600 | 54.688 | 64 | 27 | 1 | 25 | 86 | 31 | 94 | 1.94e-18 | 76.6 |
| MS.gene018941.t1 | AT5G15210 | 57.143 | 63 | 25 | 1 | 25 | 85 | 56 | 118 | 2.41e-18 | 77.0 |
| MS.gene018941.t1 | AT5G42780 | 58.333 | 60 | 23 | 1 | 19 | 78 | 58 | 115 | 2.63e-17 | 73.6 |
| MS.gene018941.t1 | AT3G28920 | 54.412 | 68 | 27 | 2 | 23 | 88 | 50 | 115 | 2.12e-16 | 72.0 |
| MS.gene018941.t1 | AT5G60480 | 47.761 | 67 | 33 | 1 | 23 | 87 | 35 | 101 | 1.70e-15 | 68.6 |
| MS.gene018941.t1 | AT5G39760 | 53.125 | 64 | 28 | 1 | 25 | 86 | 56 | 119 | 6.86e-15 | 68.2 |
| MS.gene018941.t1 | AT1G14687 | 55.102 | 49 | 17 | 2 | 25 | 73 | 7 | 50 | 2.89e-13 | 61.6 |
Find 19 sgRNAs with CRISPR-Local
Find 16 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGATATCATCGACCATAATT+AGG | 0.283610 | 3.1:+27683088 | None:intergenic |
| AAGAATCATGCAGCAAATAT+TGG | 0.303529 | 3.1:-27683256 | MS.gene018941:CDS |
| ATTACAAGAAATATTAGATA+TGG | 0.363803 | 3.1:-27683289 | MS.gene018941:CDS |
| GAGTACTCTCCACCTAATTA+TGG | 0.393626 | 3.1:-27683100 | MS.gene018941:CDS |
| GGTATGCTGTAGATGGTTGC+AGG | 0.408709 | 3.1:-27683231 | MS.gene018941:CDS |
| AGATGGTTGCAGGGAGTTCA+TGG | 0.409911 | 3.1:-27683221 | MS.gene018941:CDS |
| AGTTGTGGTGAAAAGAGATA+TGG | 0.462976 | 3.1:-27683329 | MS.gene018941:CDS |
| GTGCTGCATGTGGTTGTCAT+AGG | 0.470137 | 3.1:-27683162 | MS.gene018941:CDS |
| ATTGGAGGGTATGCTGTAGA+TGG | 0.520125 | 3.1:-27683238 | MS.gene018941:CDS |
| TTCATGGCAAGTACAGAAGA+AGG | 0.570746 | 3.1:-27683205 | MS.gene018941:CDS |
| AATCATGCAGCAAATATTGG+AGG | 0.572292 | 3.1:-27683253 | MS.gene018941:CDS |
| GATGAAGAAGAGACAAGTTG+TGG | 0.602168 | 3.1:-27683344 | MS.gene018941:CDS |
| GCTCTTACATGTGCTGCATG+TGG | 0.619232 | 3.1:-27683172 | MS.gene018941:CDS |
| ATCATGCAGCAAATATTGGA+GGG | 0.624158 | 3.1:-27683252 | MS.gene018941:CDS |
| TATCATCGACCATAATTAGG+TGG | 0.628535 | 3.1:+27683091 | None:intergenic |
| GTATGCTGTAGATGGTTGCA+GGG | 0.640365 | 3.1:-27683230 | MS.gene018941:CDS |
| AGTACAGAAGAAGGAACAAG+TGG | 0.644860 | 3.1:-27683196 | MS.gene018941:CDS |
| GTACAGAAGAAGGAACAAGT+GGG | 0.687829 | 3.1:-27683195 | MS.gene018941:CDS |
| TACAGAAGAAGGAACAAGTG+GGG | 0.696853 | 3.1:-27683194 | MS.gene018941:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTACAAGAAATATTAGATA+TGG | - | chr3.1:27683147-27683166 | MS.gene018941:CDS | 15.0% |
| AAGAATCATGCAGCAAATAT+TGG | - | chr3.1:27683180-27683199 | MS.gene018941:CDS | 30.0% | |
| AATCATGCAGCAAATATTGG+AGG | - | chr3.1:27683183-27683202 | MS.gene018941:CDS | 35.0% | |
| AGTTGTGGTGAAAAGAGATA+TGG | - | chr3.1:27683107-27683126 | MS.gene018941:CDS | 35.0% | |
| ATCATGCAGCAAATATTGGA+GGG | - | chr3.1:27683184-27683203 | MS.gene018941:CDS | 35.0% | |
| AGTACAGAAGAAGGAACAAG+TGG | - | chr3.1:27683240-27683259 | MS.gene018941:CDS | 40.0% | |
| GAGTACTCTCCACCTAATTA+TGG | - | chr3.1:27683336-27683355 | MS.gene018941:CDS | 40.0% | |
| GTACAGAAGAAGGAACAAGT+GGG | - | chr3.1:27683241-27683260 | MS.gene018941:CDS | 40.0% | |
| TACAGAAGAAGGAACAAGTG+GGG | - | chr3.1:27683242-27683261 | MS.gene018941:CDS | 40.0% | |
| TTCATGGCAAGTACAGAAGA+AGG | - | chr3.1:27683231-27683250 | MS.gene018941:CDS | 40.0% | |
| GTATGCTGTAGATGGTTGCA+GGG | - | chr3.1:27683206-27683225 | MS.gene018941:CDS | 45.0% | |
| ! | ATTGGAGGGTATGCTGTAGA+TGG | - | chr3.1:27683198-27683217 | MS.gene018941:CDS | 45.0% |
| AGATGGTTGCAGGGAGTTCA+TGG | - | chr3.1:27683215-27683234 | MS.gene018941:CDS | 50.0% | |
| GCTCTTACATGTGCTGCATG+TGG | - | chr3.1:27683264-27683283 | MS.gene018941:CDS | 50.0% | |
| GGTATGCTGTAGATGGTTGC+AGG | - | chr3.1:27683205-27683224 | MS.gene018941:CDS | 50.0% | |
| GTGCTGCATGTGGTTGTCAT+AGG | - | chr3.1:27683274-27683293 | MS.gene018941:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 27683093 | 27683365 | 27683093 | ID=MS.gene018941 |
| chr3.1 | mRNA | 27683093 | 27683365 | 27683093 | ID=MS.gene018941.t1;Parent=MS.gene018941 |
| chr3.1 | exon | 27683093 | 27683365 | 27683093 | ID=MS.gene018941.t1.exon1;Parent=MS.gene018941.t1 |
| chr3.1 | CDS | 27683093 | 27683365 | 27683093 | ID=cds.MS.gene018941.t1;Parent=MS.gene018941.t1 |
| Gene Sequence |
| Protein sequence |
