Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018978.t1 | KEH33404.1 | 78.8 | 184 | 33 | 3 | 27 | 204 | 3 | 186 | 2.00E-68 | 268.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018978.t1 | A0A072UUA3 | 78.8 | 184 | 33 | 3 | 27 | 204 | 3 | 186 | 1.4e-68 | 268.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene018978.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018978.t1 | MTR_3g437790 | 82.609 | 184 | 26 | 3 | 27 | 204 | 3 | 186 | 5.31e-98 | 282 |
| MS.gene018978.t1 | MTR_2g045020 | 62.162 | 185 | 62 | 6 | 18 | 198 | 1 | 181 | 1.53e-60 | 187 |
| MS.gene018978.t1 | MTR_8g022970 | 51.429 | 175 | 72 | 6 | 32 | 197 | 10 | 180 | 5.00e-47 | 153 |
| MS.gene018978.t1 | MTR_3g109930 | 52.318 | 151 | 68 | 3 | 48 | 196 | 16 | 164 | 1.21e-43 | 144 |
| MS.gene018978.t1 | MTR_2g105290 | 36.646 | 161 | 94 | 3 | 31 | 185 | 3 | 161 | 2.88e-24 | 94.4 |
| MS.gene018978.t1 | MTR_7g028448 | 36.842 | 133 | 74 | 2 | 53 | 185 | 31 | 153 | 2.08e-21 | 87.0 |
| MS.gene018978.t1 | MTR_4g036915 | 36.842 | 133 | 74 | 2 | 53 | 185 | 31 | 153 | 2.08e-21 | 87.0 |
| MS.gene018978.t1 | MTR_8g086290 | 34.395 | 157 | 95 | 3 | 35 | 185 | 7 | 161 | 1.06e-19 | 82.8 |
| MS.gene018978.t1 | MTR_5g053390 | 36.774 | 155 | 86 | 4 | 38 | 182 | 7 | 159 | 2.12e-18 | 80.5 |
| MS.gene018978.t1 | MTR_4g127140 | 34.507 | 142 | 83 | 3 | 53 | 185 | 31 | 171 | 5.06e-18 | 78.6 |
| MS.gene018978.t1 | MTR_3g052920 | 32.911 | 158 | 88 | 3 | 38 | 182 | 7 | 159 | 5.91e-16 | 73.9 |
| MS.gene018978.t1 | MTR_8g043650 | 30.909 | 165 | 95 | 4 | 31 | 182 | 1 | 159 | 1.07e-15 | 73.2 |
| MS.gene018978.t1 | MTR_1g012570 | 32.298 | 161 | 85 | 5 | 38 | 182 | 7 | 159 | 2.33e-15 | 72.4 |
| MS.gene018978.t1 | MTR_1g105920 | 31.646 | 158 | 90 | 4 | 38 | 182 | 7 | 159 | 2.95e-15 | 72.0 |
| MS.gene018978.t1 | MTR_1g106070 | 31.013 | 158 | 91 | 3 | 38 | 182 | 7 | 159 | 3.62e-15 | 70.9 |
| MS.gene018978.t1 | MTR_3g030770 | 33.125 | 160 | 83 | 5 | 39 | 182 | 8 | 159 | 6.10e-15 | 70.9 |
| MS.gene018978.t1 | MTR_2g085250 | 29.375 | 160 | 91 | 4 | 38 | 182 | 7 | 159 | 7.23e-15 | 70.9 |
| MS.gene018978.t1 | MTR_2g085280 | 29.375 | 160 | 91 | 4 | 38 | 182 | 7 | 159 | 7.38e-15 | 70.9 |
| MS.gene018978.t1 | MTR_3g080940 | 29.861 | 144 | 83 | 3 | 53 | 186 | 28 | 163 | 1.10e-14 | 70.1 |
| MS.gene018978.t1 | MTR_1g105910 | 31.013 | 158 | 91 | 3 | 38 | 182 | 7 | 159 | 1.21e-14 | 70.5 |
| MS.gene018978.t1 | MTR_2g030740 | 32.298 | 161 | 85 | 5 | 38 | 182 | 7 | 159 | 5.13e-14 | 68.6 |
| MS.gene018978.t1 | MTR_1g108510 | 32.500 | 160 | 84 | 5 | 39 | 182 | 8 | 159 | 5.63e-14 | 68.6 |
| MS.gene018978.t1 | MTR_8g046350 | 42.466 | 73 | 36 | 1 | 41 | 107 | 3 | 75 | 1.67e-13 | 65.9 |
| MS.gene018978.t1 | MTR_1g108580 | 32.500 | 160 | 84 | 5 | 39 | 182 | 8 | 159 | 2.12e-13 | 67.0 |
| MS.gene018978.t1 | MTR_5g055100 | 29.878 | 164 | 83 | 4 | 38 | 181 | 7 | 158 | 2.77e-13 | 66.6 |
| MS.gene018978.t1 | MTR_8g051580 | 46.552 | 58 | 31 | 0 | 53 | 110 | 21 | 78 | 8.97e-12 | 61.6 |
| MS.gene018978.t1 | MTR_3g052870 | 43.056 | 72 | 35 | 1 | 38 | 103 | 7 | 78 | 9.35e-12 | 62.0 |
| MS.gene018978.t1 | MTR_0121s0080 | 33.824 | 136 | 78 | 4 | 54 | 182 | 29 | 159 | 1.64e-11 | 61.6 |
| MS.gene018978.t1 | MTR_1g047550 | 41.096 | 73 | 37 | 1 | 41 | 107 | 3 | 75 | 1.70e-11 | 60.8 |
| MS.gene018978.t1 | MTR_1g105905 | 30.216 | 139 | 79 | 4 | 53 | 181 | 11 | 141 | 1.94e-11 | 61.2 |
| MS.gene018978.t1 | MTR_8g079502 | 42.647 | 68 | 33 | 1 | 41 | 102 | 3 | 70 | 1.97e-11 | 60.8 |
| MS.gene018978.t1 | MTR_1g077360 | 29.193 | 161 | 90 | 3 | 38 | 182 | 7 | 159 | 2.08e-11 | 61.6 |
| MS.gene018978.t1 | MTR_2g093190 | 28.931 | 159 | 91 | 3 | 39 | 182 | 8 | 159 | 4.07e-11 | 60.5 |
| MS.gene018978.t1 | MTR_7g062350 | 33.577 | 137 | 79 | 3 | 53 | 182 | 40 | 171 | 6.75e-11 | 60.1 |
| MS.gene018978.t1 | MTR_1g063160 | 42.647 | 68 | 33 | 1 | 41 | 102 | 3 | 70 | 9.67e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene018978.t1 | AT5G60440 | 31.757 | 148 | 92 | 4 | 53 | 194 | 26 | 170 | 8.78e-17 | 77.0 |
| MS.gene018978.t1 | AT2G24840 | 28.090 | 178 | 106 | 6 | 25 | 192 | 63 | 228 | 4.63e-16 | 74.7 |
| MS.gene018978.t1 | AT1G65360 | 30.345 | 145 | 82 | 5 | 38 | 169 | 5 | 143 | 5.57e-12 | 63.2 |
| MS.gene018978.t1 | AT4G36590 | 28.571 | 161 | 97 | 5 | 38 | 182 | 5 | 163 | 6.00e-12 | 63.2 |
| MS.gene018978.t1 | AT3G66656 | 51.020 | 49 | 24 | 0 | 53 | 101 | 21 | 69 | 3.30e-11 | 60.1 |
| MS.gene018978.t1 | AT2G34440 | 29.508 | 122 | 80 | 2 | 53 | 171 | 21 | 139 | 3.70e-11 | 59.7 |
Find 32 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGCTTCATATCGAAGTCTT+AGG | 0.201474 | 3.3:+29639451 | None:intergenic |
| TAACACACATTCTCTTGAAT+TGG | 0.284587 | 3.3:+29639225 | None:intergenic |
| GAATAAATCTATTGATGATA+TGG | 0.314688 | 3.3:-29639340 | MS.gene018978:CDS |
| TGATATTGAATGAAACCAAC+TGG | 0.389424 | 3.3:-29639794 | MS.gene018978:CDS |
| CGCTTCATATCGAAGTCTTA+GGG | 0.401643 | 3.3:+29639452 | None:intergenic |
| TTGTTTCTCTCGGCCGCCAT+TGG | 0.404729 | 3.3:+29639729 | None:intergenic |
| TGAATGACAGCGTCACAGTT+AGG | 0.413803 | 3.3:+29639543 | None:intergenic |
| CTTCCGTGTGTTGTTTCTCT+CGG | 0.413838 | 3.3:+29639719 | None:intergenic |
| CGATGAGTGCAGTTTCTGAT+TGG | 0.423120 | 3.3:+29639604 | None:intergenic |
| CTCATCCTCGTCCATATGTC+CGG | 0.445428 | 3.3:+29639509 | None:intergenic |
| CTTATTCATGTTGTCCCAGT+TGG | 0.447593 | 3.3:+29639779 | None:intergenic |
| CTTCTTCGCTTCGCTTGCTA+CGG | 0.451335 | 3.3:+29639260 | None:intergenic |
| CTTCGATATGAAGCGATTCA+AGG | 0.460946 | 3.3:-29639444 | MS.gene018978:CDS |
| GAATGACAGCGTCACAGTTA+GGG | 0.477256 | 3.3:+29639544 | None:intergenic |
| GAAGGATGAAATCAAGAAAG+AGG | 0.477445 | 3.3:-29639691 | MS.gene018978:CDS |
| GATTTATTCCACCAATCAGC+AGG | 0.485193 | 3.3:+29639354 | None:intergenic |
| CTCTTCTTCAAGATCATGGA+TGG | 0.520427 | 3.3:-29640049 | None:intergenic |
| CATGCATAGAACTTGTTGTT+GGG | 0.522753 | 3.3:+29639573 | None:intergenic |
| AGGAAAACTTAAAGAAGAGA+AGG | 0.524858 | 3.3:-29639424 | MS.gene018978:CDS |
| CAACACACGGAAGAGAAAGA+AGG | 0.527351 | 3.3:-29639709 | MS.gene018978:CDS |
| CCGAGTTTGAGTTTCGAGAA+GGG | 0.532852 | 3.3:+29639663 | None:intergenic |
| TTGATTTGCCTGCTGATTGG+TGG | 0.538450 | 3.3:-29639362 | MS.gene018978:CDS |
| CCCTTCTCGAAACTCAAACT+CGG | 0.552196 | 3.3:-29639663 | MS.gene018978:CDS |
| TGGGACAACATGAATAAGAA+TGG | 0.556319 | 3.3:-29639774 | MS.gene018978:CDS |
| TCCGAGTTTGAGTTTCGAGA+AGG | 0.567859 | 3.3:+29639662 | None:intergenic |
| ACATGCATAGAACTTGTTGT+TGG | 0.594378 | 3.3:+29639572 | None:intergenic |
| AACAACAAGTTCTATGCATG+TGG | 0.619639 | 3.3:-29639570 | MS.gene018978:CDS |
| GTCTAAGAAGAAAATTCCAA+TGG | 0.626985 | 3.3:-29639745 | MS.gene018978:CDS |
| TAAGAAGAAAATTCCAATGG+CGG | 0.679668 | 3.3:-29639742 | MS.gene018978:CDS |
| CGGCCGAGAGAAACAACACA+CGG | 0.685523 | 3.3:-29639722 | MS.gene018978:CDS |
| TCTGACCGGACATATGGACG+AGG | 0.696130 | 3.3:-29639514 | MS.gene018978:CDS |
| GATATTGAATGAAACCAACT+GGG | 0.707052 | 3.3:-29639793 | MS.gene018978:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAATAAATCTATTGATGATA+TGG | - | chr3.3:29639918-29639937 | MS.gene018978:intron | 20.0% |
| !!! | TTGTTTTATGTGAATAAACT+TGG | + | chr3.3:29639363-29639382 | None:intergenic | 20.0% |
| AGGAAAACTTAAAGAAGAGA+AGG | - | chr3.3:29639834-29639853 | MS.gene018978:intron | 30.0% | |
| GATATTGAATGAAACCAACT+GGG | - | chr3.3:29639465-29639484 | MS.gene018978:CDS | 30.0% | |
| GTCTAAGAAGAAAATTCCAA+TGG | - | chr3.3:29639513-29639532 | MS.gene018978:CDS | 30.0% | |
| TAACACACATTCTCTTGAAT+TGG | + | chr3.3:29640036-29640055 | None:intergenic | 30.0% | |
| TAAGAAGAAAATTCCAATGG+CGG | - | chr3.3:29639516-29639535 | MS.gene018978:CDS | 30.0% | |
| TGATATTGAATGAAACCAAC+TGG | - | chr3.3:29639464-29639483 | MS.gene018978:CDS | 30.0% | |
| TTATGTGAATAAACTTGGCT+AGG | + | chr3.3:29639358-29639377 | None:intergenic | 30.0% | |
| AACAACAAGTTCTATGCATG+TGG | - | chr3.3:29639688-29639707 | MS.gene018978:CDS | 35.0% | |
| GAAGGATGAAATCAAGAAAG+AGG | - | chr3.3:29639567-29639586 | MS.gene018978:CDS | 35.0% | |
| TGGGACAACATGAATAAGAA+TGG | - | chr3.3:29639484-29639503 | MS.gene018978:CDS | 35.0% | |
| TGTGTGATTTCAGACTTTGT+GGG | - | chr3.3:29639388-29639407 | MS.gene018978:CDS | 35.0% | |
| ! | TGATTAGGGTTTTTACCCTA+TGG | - | chr3.3:29639332-29639351 | MS.gene018978:CDS | 35.0% |
| !! | ACATGCATAGAACTTGTTGT+TGG | + | chr3.3:29639689-29639708 | None:intergenic | 35.0% |
| !! | CATGCATAGAACTTGTTGTT+GGG | + | chr3.3:29639688-29639707 | None:intergenic | 35.0% |
| !!! | ATTTTGATTTGCCTGCTGAT+TGG | - | chr3.3:29639893-29639912 | MS.gene018978:intron | 35.0% |
| AATAAACTTGGCTAGGCCAT+AGG | + | chr3.3:29639351-29639370 | None:intergenic | 40.0% | |
| ATAAACTTGGCTAGGCCATA+GGG | + | chr3.3:29639350-29639369 | None:intergenic | 40.0% | |
| CGCTTCATATCGAAGTCTTA+GGG | + | chr3.3:29639809-29639828 | None:intergenic | 40.0% | |
| CTTATTCATGTTGTCCCAGT+TGG | + | chr3.3:29639482-29639501 | None:intergenic | 40.0% | |
| CTTCGATATGAAGCGATTCA+AGG | - | chr3.3:29639814-29639833 | MS.gene018978:intron | 40.0% | |
| GATTTATTCCACCAATCAGC+AGG | + | chr3.3:29639907-29639926 | None:intergenic | 40.0% | |
| GTGTGTGATTTCAGACTTTG+TGG | - | chr3.3:29639387-29639406 | MS.gene018978:CDS | 40.0% | |
| TCGCTTCATATCGAAGTCTT+AGG | + | chr3.3:29639810-29639829 | None:intergenic | 40.0% | |
| ! | AACCAAGAAAGTGCGTGATT+AGG | - | chr3.3:29639317-29639336 | MS.gene018978:CDS | 40.0% |
| ! | ACCAAGAAAGTGCGTGATTA+GGG | - | chr3.3:29639318-29639337 | MS.gene018978:CDS | 40.0% |
| ! | GTCATTCAACGTTTTCTGAC+CGG | - | chr3.3:29639730-29639749 | MS.gene018978:CDS | 40.0% |
| ACCCTAATCACGCACTTTCT+TGG | + | chr3.3:29639322-29639341 | None:intergenic | 45.0% | |
| CAACACACGGAAGAGAAAGA+AGG | - | chr3.3:29639549-29639568 | MS.gene018978:CDS | 45.0% | |
| CCCTTCTCGAAACTCAAACT+CGG | - | chr3.3:29639595-29639614 | MS.gene018978:CDS | 45.0% | |
| CGATGAGTGCAGTTTCTGAT+TGG | + | chr3.3:29639657-29639676 | None:intergenic | 45.0% | |
| GAATGACAGCGTCACAGTTA+GGG | + | chr3.3:29639717-29639736 | None:intergenic | 45.0% | |
| TGAATGACAGCGTCACAGTT+AGG | + | chr3.3:29639718-29639737 | None:intergenic | 45.0% | |
| ! | ACGTTTTCTGACCGGACATA+TGG | - | chr3.3:29639738-29639757 | MS.gene018978:CDS | 45.0% |
| ! | CCGAGTTTGAGTTTCGAGAA+GGG | + | chr3.3:29639598-29639617 | None:intergenic | 45.0% |
| ! | TCCGAGTTTGAGTTTCGAGA+AGG | + | chr3.3:29639599-29639618 | None:intergenic | 45.0% |
| !! | CTTCCGTGTGTTGTTTCTCT+CGG | + | chr3.3:29639542-29639561 | None:intergenic | 45.0% |
| !! | TTGATTTGCCTGCTGATTGG+TGG | - | chr3.3:29639896-29639915 | MS.gene018978:intron | 45.0% |
| CTCATCCTCGTCCATATGTC+CGG | + | chr3.3:29639752-29639771 | None:intergenic | 50.0% | |
| ! | CTTCTTCGCTTCGCTTGCTA+CGG | + | chr3.3:29640001-29640020 | None:intergenic | 50.0% |
| CGGCCGAGAGAAACAACACA+CGG | - | chr3.3:29639536-29639555 | MS.gene018978:CDS | 55.0% | |
| TCTGACCGGACATATGGACG+AGG | - | chr3.3:29639744-29639763 | MS.gene018978:CDS | 55.0% | |
| ! | TTGTTTCTCTCGGCCGCCAT+TGG | + | chr3.3:29639532-29639551 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 29639224 | 29640056 | 29639224 | ID=MS.gene018978 |
| chr3.3 | mRNA | 29639224 | 29640056 | 29639224 | ID=MS.gene018978.t1;Parent=MS.gene018978 |
| chr3.3 | exon | 29640050 | 29640056 | 29640050 | ID=MS.gene018978.t1.exon1;Parent=MS.gene018978.t1 |
| chr3.3 | CDS | 29640050 | 29640056 | 29640050 | ID=cds.MS.gene018978.t1;Parent=MS.gene018978.t1 |
| chr3.3 | exon | 29639224 | 29639831 | 29639224 | ID=MS.gene018978.t1.exon2;Parent=MS.gene018978.t1 |
| chr3.3 | CDS | 29639224 | 29639831 | 29639224 | ID=cds.MS.gene018978.t1;Parent=MS.gene018978.t1 |
| Gene Sequence |
| Protein sequence |
